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Fundamentals of light microscopy and electronic imaging / / Douglas B. Murphy, Michael W. Davidson
Fundamentals of light microscopy and electronic imaging / / Douglas B. Murphy, Michael W. Davidson
Autore Murphy Douglas B
Edizione [2nd ed.]
Pubbl/distr/stampa Hoboken, N.J., : Wiley-Blackwell, 2012
Descrizione fisica 1 online resource (xiii, 538 p.)
Disciplina 502.8/2
Altri autori (Persone) DavidsonMichael W <1950-> (Michael Wesley)
Soggetto topico Microscòpia
Imatges - Processament - Tècniques digitals
Microscopy
Image processing - Digital techniques
ISBN 1-283-64427-4
1-118-38290-0
1-118-38291-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Title page; Copyright page; Contents; Preface; Acknowledgments; CHAPTER 1: Fundamentals of Light Microscopy; Overview; Optical Components of the Light Microscope; Aperture and Image Planes in a Focused, Adjusted Microscope; Koehler Illumination; Adjusting the Microscope for Koehler Illumination; Fixed Tube Length versus Infinity Optical Systems; Precautions for Handling Optical Equipment; Care and Maintenance of the Microscope; CHAPTER 2: Light and Color; Overview; Light as a Probe of Matter; The Dual Particle- and Wave-Like Nature of Light; The Quality of Light
Properties of Light Perceived by the EyePhysical Basis for Visual Perception and Color; Addition and Subtraction Colors; CHAPTER 3: Illuminators, Filters, and the Isolation of Specific Wavelengths; Overview; Illuminators and Their Spectra; Illuminator Alignment and Bulb Replacement; Filters for Adjusting the Intensity and Wavelength of Illumination; Neutral Density Filters; Colored Glass Filters; Interference Filters; Effects of Light on Living Cells; CHAPTER 4: Lenses and Geometrical Optics; Overview; Reflection and Refraction of Light; Image Formation by a Simple Lens
Rules of Ray Tracing for a Simple LensObject-Image Math; The Principal Aberrations of Lenses; Designs and Specifications of Objectives; Special Objective Designs; Markings on the Barrel of an Objective; Image Brightness; Objective Parfocality; Condensers; Oculars; Microscope Slides and Coverslips; The Care and Cleaning of Optics; Dust; Immersion Oil; Scratches and Abrasions; Mechanical Force; CHAPTER 5: Diffraction and Interference in Image Formation; Overview; Diffraction and Interference; The Diffraction Image of a Point Source of Light
The Constancy of Optical Path Length between Object and ImageEffect of Aperture Angle on Diffraction Spot Size; Diffraction by a Grating and Calculation of Its Line Spacing, D; Abbé's Theory for Image Formation in the Microscope; A Diffraction Pattern Is Formed in the Rear Aperture of the Objective; Preservation of Coherence: Essential Requirement for Image Formation; CHAPTER 6: Diffraction and Spatial Resolution; Overview; Numerical Aperture; Spatial Resolution; Depth of Field and Depth of Focus; Optimizing the Microscope Image: A Compromise between Spatial Resolution and Contrast
CHAPTER 7: Phase Contrast Microscopy and Darkfield MicroscopyOverview; Phase Contrast Microscopy; The Behavior of Waves from Phase Objects in Brightfield Microscopy; Wave Terminology and the Importance of Coherence; Depiction of Wave Interactions with Sine Wave and Vector Diagrams; The Role of Differences in Optical Path Lengths; The Optical Design of the Phase Contrast Microscope; Alignment; Interpreting the Phase Contrast Image; Darkfield Microscopy; Theory and Optics; Image Interpretation; CHAPTER 8: Properties of Polarized Light; Overview; The Generation of Polarized Light
Polarization by Reflection and Scattering
Record Nr. UNINA-9910141379403321
Murphy Douglas B  
Hoboken, N.J., : Wiley-Blackwell, 2012
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Invertebrate biology
Invertebrate biology
Pubbl/distr/stampa Lawrence, Kan., : Allen Press, 1995-
Descrizione fisica 1 online resource
Disciplina 592.005
Soggetto topico Invertebrates
Microscopy
Invertébrés
Microscopie
Invertebrats
Zoologia
Microscòpia
Soggetto genere / forma Periodicals.
Revistes electròniques.
ISSN 1744-7410
Formato Materiale a stampa
Livello bibliografico Periodico
Lingua di pubblicazione eng
Record Nr. UNINA-9910144572903321
Lawrence, Kan., : Allen Press, 1995-
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Microscopy research and technique
Microscopy research and technique
Pubbl/distr/stampa New York, NY, : Wiley-Liss
Disciplina 502
Soggetto topico Electron microscopy - Technique
Microscopy
Microscopy - Technique
Microscopy - Technique - Periodicals
Diagnostic imaging
Electron microscopy
Diagnostic Imaging
Microscopy, Electron
Microscopie
Microscopie - Technique - Périodiques
Microscopie électronique - Technique
Microscopie - Technique
Imagerie pour le diagnostic
Microscopie électronique
electron microscopy
Microscòpia electrònica
Microscòpia
Soggetto genere / forma Fulltext
Internet Resources.
Periodicals.
Revistes electròniques.
ISSN 1097-0029
Formato Materiale a stampa
Livello bibliografico Periodico
Lingua di pubblicazione eng
Altri titoli varianti Microscopy research & technique
Record Nr. UNINA-9910144004003321
New York, NY, : Wiley-Liss
Materiale a stampa
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Principles of Light Microscopy: From Basic to Advanced / / edited by Volodymyr Nechyporuk-Zloy
Principles of Light Microscopy: From Basic to Advanced / / edited by Volodymyr Nechyporuk-Zloy
Edizione [1st ed. 2022.]
Pubbl/distr/stampa Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022
Descrizione fisica 1 online resource (327 pages)
Disciplina 578
570.282
Soggetto topico Medicine—Research
Biology—Research
Life sciences
Cytology
Spectrum analysis
Biomedical Research
Life Sciences
Cell Biology
Spectroscopy
Microscòpia
Soggetto genere / forma Llibres electrònics
ISBN 9783031044779
9783031044762
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Chapter 1. The physical principles of light propagation and light-matter interactions -- Chapter 2. Design, alignment and usage of infinity-corrected microscope -- Chapter 3. Epifluorescence Microscopy -- Chapter 4. Basic digital image design, processing, analysis, management, and presentation -- Chapter 5. Confocal Microscopy -- Chapter 6. Live-cell Imaging: A Balancing Act Between Speed, Sensitivity and Resolution -- Chapter 7. Structured Illumination Microscopy -- Chapter 8. Stimulated Emission Depletion Microscopy -- Chapter 9. Two-Photon Imaging -- Chapter 10. Modern microscopy image analysis: quantifying colocalization on a mobile device -- Chapter 11. Spinning Disk Microscopy -- Chapter 12. Light Sheet Fluorescence Microscopy -- Chapter 13. Localization microscopy: a review of the progress in methods and applications.
Record Nr. UNINA-9910633919403321
Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022
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