Fundamentals of light microscopy and electronic imaging / / Douglas B. Murphy, Michael W. Davidson
| Fundamentals of light microscopy and electronic imaging / / Douglas B. Murphy, Michael W. Davidson |
| Autore | Murphy Douglas B |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Blackwell, 2012 |
| Descrizione fisica | 1 online resource (xiii, 538 p.) |
| Disciplina | 502.8/2 |
| Altri autori (Persone) | DavidsonMichael W <1950-> (Michael Wesley) |
| Soggetto topico |
Microscòpia
Imatges - Processament - Tècniques digitals Microscopy Image processing - Digital techniques |
| ISBN |
1-283-64427-4
1-118-38290-0 1-118-38291-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Title page; Copyright page; Contents; Preface; Acknowledgments; CHAPTER 1: Fundamentals of Light Microscopy; Overview; Optical Components of the Light Microscope; Aperture and Image Planes in a Focused, Adjusted Microscope; Koehler Illumination; Adjusting the Microscope for Koehler Illumination; Fixed Tube Length versus Infinity Optical Systems; Precautions for Handling Optical Equipment; Care and Maintenance of the Microscope; CHAPTER 2: Light and Color; Overview; Light as a Probe of Matter; The Dual Particle- and Wave-Like Nature of Light; The Quality of Light
Properties of Light Perceived by the EyePhysical Basis for Visual Perception and Color; Addition and Subtraction Colors; CHAPTER 3: Illuminators, Filters, and the Isolation of Specific Wavelengths; Overview; Illuminators and Their Spectra; Illuminator Alignment and Bulb Replacement; Filters for Adjusting the Intensity and Wavelength of Illumination; Neutral Density Filters; Colored Glass Filters; Interference Filters; Effects of Light on Living Cells; CHAPTER 4: Lenses and Geometrical Optics; Overview; Reflection and Refraction of Light; Image Formation by a Simple Lens Rules of Ray Tracing for a Simple LensObject-Image Math; The Principal Aberrations of Lenses; Designs and Specifications of Objectives; Special Objective Designs; Markings on the Barrel of an Objective; Image Brightness; Objective Parfocality; Condensers; Oculars; Microscope Slides and Coverslips; The Care and Cleaning of Optics; Dust; Immersion Oil; Scratches and Abrasions; Mechanical Force; CHAPTER 5: Diffraction and Interference in Image Formation; Overview; Diffraction and Interference; The Diffraction Image of a Point Source of Light The Constancy of Optical Path Length between Object and ImageEffect of Aperture Angle on Diffraction Spot Size; Diffraction by a Grating and Calculation of Its Line Spacing, D; Abbé's Theory for Image Formation in the Microscope; A Diffraction Pattern Is Formed in the Rear Aperture of the Objective; Preservation of Coherence: Essential Requirement for Image Formation; CHAPTER 6: Diffraction and Spatial Resolution; Overview; Numerical Aperture; Spatial Resolution; Depth of Field and Depth of Focus; Optimizing the Microscope Image: A Compromise between Spatial Resolution and Contrast CHAPTER 7: Phase Contrast Microscopy and Darkfield MicroscopyOverview; Phase Contrast Microscopy; The Behavior of Waves from Phase Objects in Brightfield Microscopy; Wave Terminology and the Importance of Coherence; Depiction of Wave Interactions with Sine Wave and Vector Diagrams; The Role of Differences in Optical Path Lengths; The Optical Design of the Phase Contrast Microscope; Alignment; Interpreting the Phase Contrast Image; Darkfield Microscopy; Theory and Optics; Image Interpretation; CHAPTER 8: Properties of Polarized Light; Overview; The Generation of Polarized Light Polarization by Reflection and Scattering |
| Record Nr. | UNINA-9910141379403321 |
Murphy Douglas B
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| Hoboken, N.J., : Wiley-Blackwell, 2012 | ||
| Lo trovi qui: Univ. Federico II | ||
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Invertebrate biology
| Invertebrate biology |
| Pubbl/distr/stampa | Lawrence, Kan., : Allen Press, 1995- |
| Descrizione fisica | 1 online resource |
| Disciplina | 592.005 |
| Soggetto topico |
Invertebrates
Microscopy Invertébrés Microscopie Invertebrats Zoologia Microscòpia |
| Soggetto genere / forma |
Periodicals.
Revistes electròniques. |
| ISSN | 1744-7410 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910144572903321 |
| Lawrence, Kan., : Allen Press, 1995- | ||
| Lo trovi qui: Univ. Federico II | ||
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Microscopy research and technique
| Microscopy research and technique |
| Pubbl/distr/stampa | New York, NY, : Wiley-Liss |
| Disciplina | 502 |
| Soggetto topico |
Electron microscopy - Technique
Microscopy Microscopy - Technique Microscopy - Technique - Periodicals Diagnostic imaging Electron microscopy Diagnostic Imaging Microscopy, Electron Microscopie Microscopie - Technique - Périodiques Microscopie électronique - Technique Microscopie - Technique Imagerie pour le diagnostic Microscopie électronique electron microscopy Microscòpia electrònica Microscòpia |
| Soggetto genere / forma |
Fulltext
Internet Resources. Periodicals. Revistes electròniques. |
| ISSN | 1097-0029 |
| Formato | Materiale a stampa |
| Livello bibliografico | Periodico |
| Lingua di pubblicazione | eng |
| Altri titoli varianti | Microscopy research & technique |
| Record Nr. | UNINA-9910144004003321 |
| New York, NY, : Wiley-Liss | ||
| Lo trovi qui: Univ. Federico II | ||
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Principles of Light Microscopy: From Basic to Advanced / / edited by Volodymyr Nechyporuk-Zloy
| Principles of Light Microscopy: From Basic to Advanced / / edited by Volodymyr Nechyporuk-Zloy |
| Edizione | [1st ed. 2022.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 |
| Descrizione fisica | 1 online resource (327 pages) |
| Disciplina |
578
570.282 |
| Soggetto topico |
Medicine—Research
Biology—Research Life sciences Cytology Spectrum analysis Biomedical Research Life Sciences Cell Biology Spectroscopy Microscòpia |
| Soggetto genere / forma | Llibres electrònics |
| ISBN |
9783031044779
9783031044762 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Chapter 1. The physical principles of light propagation and light-matter interactions -- Chapter 2. Design, alignment and usage of infinity-corrected microscope -- Chapter 3. Epifluorescence Microscopy -- Chapter 4. Basic digital image design, processing, analysis, management, and presentation -- Chapter 5. Confocal Microscopy -- Chapter 6. Live-cell Imaging: A Balancing Act Between Speed, Sensitivity and Resolution -- Chapter 7. Structured Illumination Microscopy -- Chapter 8. Stimulated Emission Depletion Microscopy -- Chapter 9. Two-Photon Imaging -- Chapter 10. Modern microscopy image analysis: quantifying colocalization on a mobile device -- Chapter 11. Spinning Disk Microscopy -- Chapter 12. Light Sheet Fluorescence Microscopy -- Chapter 13. Localization microscopy: a review of the progress in methods and applications. |
| Record Nr. | UNINA-9910633919403321 |
| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 | ||
| Lo trovi qui: Univ. Federico II | ||
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