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Automation and basic techniques in medical microbiology / / Santi M. Mandal and Debarati Paul
| Automation and basic techniques in medical microbiology / / Santi M. Mandal and Debarati Paul |
| Autore | Mandal Santi M. |
| Pubbl/distr/stampa | Gateway East, Singapore : , : Springer, , [2022] |
| Descrizione fisica | 1 online resource (213 pages) |
| Disciplina | 616.9041 |
| Soggetto topico |
Medical microbiology
Microbiology - Technique Microbiologia mèdica Metodologia de la ciència |
| Soggetto genere / forma | Llibres electrònics |
| ISBN | 1-0716-2372-9 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Intro -- Preface -- Acknowledgements -- Contents -- About the Authors -- 1: Good Laboratory Practices -- 1.1 Introduction -- 1.2 Basic Record and Lab Note Book -- 1.3 Laboratory Safety Equipment -- 1.4 Biosafety Levels and Practices -- 2: Automation in Medical Microbiology -- 2.1 Introduction -- 2.2 Applications of Automation -- 2.3 Advantages and Disadvantages -- 2.3.1 Advantages of Using Auto-analysers -- 2.3.2 Disadvantages of Automation -- 2.4 Types of Auto-analysers -- 2.5 History of Auto-analysers -- 2.6 Laboratory Automation and Total Laboratory Automation -- 2.7 Types and Applications of Auto-analysers in Microbiology -- 2.7.1 Microbiological Specimen Processor -- 2.7.2 Routine Biochemistry Analysers -- 2.7.3 Immunology-Based Analysers -- 2.7.4 Haematology Analysers -- 2.7.5 Cell Counter -- 2.7.6 Coagulometer(s) -- 2.7.7 Additional Instrument for Haematology-Based Methods -- 2.7.8 Other Miscellaneous Analysers -- References -- 3: Manual and Automated Characterization of Multi-antibiotic-Resistant (MAR) Bacteria -- 3.1 Introduction -- 3.2 Types of Antibiotic Sensitivity Tests -- 3.2.1 Kirby-Bauer Disc Diffusion Method -- 3.2.2 The Minimum Inhibitory Concentration (MIC) Method -- 3.2.3 RAPD PCR Analysis -- 3.2.4 Multiplex PCR -- 3.2.5 Padlock PCR and Microarray Analysis -- 3.2.6 Real-Time PCR for Quantitative Data -- References -- 4: Rapid Microbial Genome Sequencing Techniques and Applications -- 4.1 Introduction -- 4.2 WGS Techniques -- 4.3 Data Analysis -- Protocol for WGS (adapted from Gautam et al. 2019) -- 4.4 Applications -- 4.5 Challenges -- References -- 5: Spectroscopy: Principle, Types and Microbiological Applications -- 5.1 Introduction -- 5.2 General Types of Spectra -- 5.2.1 Continuous Spectra -- 5.2.2 Discrete Spectra -- 5.2.2.1 Emission Line Spectra -- 5.2.2.2 Absorption Line Spectra -- 5.3 Principle of Spectroscopy.
5.4 Optical Instruments in Spectroscopy -- 5.5 Is Spectroscopy Different from Spectrometry? -- 5.6 Uses of Spectroscopy -- 5.7 Types of Spectroscopy -- 5.7.1 Ultraviolet and Visible Spectroscopy -- 5.7.1.1 Background -- 5.7.1.2 Principle -- 5.7.1.3 Applications of UV-Vis Spectroscopy -- 5.7.1.3.1 Spectroscopy in Environmental Analysis -- 5.7.1.3.2 UV-Vis Spectroscopy for Water Analysis and Environmental Applications -- 5.7.1.3.3 Spectrophotometric Analysis of Bacterial Water Contaminants -- 5.7.1.3.4 Spectrophotometers for Chlorine and Flouride Quantification -- 5.7.1.3.5 UV-Vis Spectroscopy for Geological Studies Linked to Water Contamination -- 5.7.1.3.6 Other Applications -- 5.7.2 Infrared Spectroscopy -- 5.7.2.1 Introduction -- 5.7.2.1.1 Molecular Vibrations and Vibrational Frequency -- 5.7.2.1.1.1 Vibration of Diatomic Molecules -- 5.7.2.1.1.2 Vibrational Transitions -- 5.7.2.1.1.3 Types of Vibrations (Sharma 2007) -- 5.7.2.2 Instrumentation -- 5.7.2.2.1 Source -- 5.7.2.2.2 Sample Types and Preparation -- 5.7.2.2.3 Various Types of Detectors Used -- 5.7.2.3 FTIR (Fourier Transform IR Spectrometers) -- 5.7.2.4 Advantages of FTIR -- 5.7.2.5 Applications of IR Spectroscopy -- 5.7.3 Mass Spectrometry -- 5.7.3.1 The Mass Spectrometer -- 5.7.3.2 The Nature of Mass Spectra -- 5.7.3.3 The Working Principle of a Mass Spectrometer -- 5.7.3.4 Applications of Mass Spectrometry -- 5.7.3.4.1 Analysis of Biomolecules -- 5.7.3.4.2 Analysis of Glycans -- 5.7.3.4.3 Analysis of Lipids -- 5.7.3.4.4 Analysis of Proteins and Peptides -- 5.7.3.4.5 Analysis of Oligonucleotides -- 5.7.4 Nuclear Magnetic Resonance (NMR) Spectroscopy -- 5.7.4.1 NMR Spectrum -- 5.7.4.2 NMR Spectrometers -- 5.7.4.3 Applications of NMR -- 5.8 Applications of Spectroscopy in Microbiology -- References -- 6: MALDI-TOF MS for Bacterial Identification -- 6.1 Introduction. 6.2 MALDI: Sample Preparation and Analysis -- 6.2.1 Sample Preparation -- 6.2.2 Protein Digestion -- 6.2.3 MALDI/MS Analysis -- 6.3 Uses of MALDI-TOF -- 6.4 MALDI-TOF MS-Based Antimicrobial Susceptibility Testing -- 6.4.1 Detection of Antibiotic Degradation -- 6.4.2 Identification of Biomarker for Detecting Antibiotic-Resistant Strains -- 6.4.3 Phenotypic Antibiotic Resistance Analysis of Bacterial Strains -- 6.5 Advantages and Limitations -- 6.6 Challenges -- References -- 7: Enzyme-Linked Immunosorbent Assay (ELISA) -- 7.1 Introduction -- 7.2 Indirect ELISA -- 7.2.1 Steps of Indirect ELISA -- 7.3 Direct or Sandwich ELISA -- 7.3.1 Steps of Double Antibody Sandwich (DAS) ELISA -- 7.3.2 Steps of Triple Antibody Sandwich (TAS) ELISA -- 7.4 Competitive ELISA -- 7.5 Radioimmunoassay (RIA) -- 7.5.1 Steps of RIA -- 7.6 Automated ELISA -- References -- 8: Isolation of Normal Microbiota from the Human Body and Microbial Identification -- 8.1 Introduction -- 8.2 Collection of Samples from Various Parts of the Body -- 8.3 Biochemical Tests for Identification of Bacteria -- 8.3.1 Carbohydrate Fermentation -- 8.3.2 Indole Production Test -- 8.3.3 Methyl Red Test -- 8.3.4 Voges-Proskauer Test -- 8.3.5 Citrate Utilization -- 8.3.6 Urease Test -- 8.3.7 Catalase Test -- 8.3.8 Coagulase Test -- 8.3.9 Lactophenol Cotton Blue -- 8.4 Rapid Multitest Systems -- 8.4.1 Automated Validation of Every Result (VITEK) System for Microbial Identification -- 8.4.2 Biolog: Phenotype Microarrays -- 8.4.3 Electromigration Techniques -- 8.4.4 MIDI Sherlock System for FAME Analysis -- 8.5 Computer-Aided Gene Analysis for Identification of Microbes -- 8.5.1 Ribosomal RNA Gene Sequencing -- 8.5.2 Phylogenetic Analysis -- 8.5.3 Generating Multiple Sequence Alignments -- 8.6 Conclusion -- References -- 9: Microarrays and Its Application in Medical Microbiology -- 9.1 Introduction. 9.2 Basic Principle -- 9.3 Immobilization Strategies Used for Preparing Microarrays -- 9.4 Manufacture of the Different Components of Microarrays -- 9.4.1 Oligonucleotide Synthesis -- 9.5 Properties of Fluorescence and Fluorophores -- 9.6 Measuring Fluorescence -- 9.7 Labelling Samples for Analysis of Gene Expressions -- 9.8 Labelling Strategies -- 9.8.1 Labelling Bacterial Transcripts -- 9.9 Labelling Samples for Gene Expression Microarray -- 9.10 Calculating Label Density in Probe -- 9.11 Steps for Microarray Hybridization -- 9.12 Different Slide Types for Microarray -- 9.13 Comparing Automated and Manual Hybridization (Table 9.2) -- 9.14 Imaging for Microarray System -- 9.15 Optical System for Imaging in Microarray -- 9.16 Detector System, Amplifier System and Digital Resolution for Imaging in Microarray -- 9.17 Scanners and Excitation Light System for Microarray -- 9.18 Data Analysis in Microarray -- 9.19 Normalization of Data for Correcting Experimental Variation Between Slides -- 9.20 Visualizing of Data and Clustering -- 9.21 Troubleshooting During Microarray-Based Experiments -- 9.22 Applications of Microarrays -- 9.23 Limitations of Microarray Technique -- 9.24 Conclusion and Future Direction -- References -- 10: Immunotechnology -- 10.1 Introduction -- 10.1.1 Monoclonal Antibodies: Purification and Concentrate -- 10.1.1.1 Principle -- 10.1.1.2 Method -- 10.1.2 Concentrate the Purified Antibody -- 10.1.3 Analysis and Quality Assurance -- 10.1.4 Preparation of Separation Gel -- 10.1.5 Preparation of Protein Sample and Loading -- 10.1.6 Staining and Distaining of the Gel -- 10.1.7 Quality Assurance -- 10.2 Immunoelectrophoresis -- 10.2.1 Protocol -- 10.3 Western Blotting -- 10.3.1 Required Material -- 10.3.2 Protocol -- 10.3.3 Blocking of Membrane -- 10.3.4 Binding of Primary Antibody -- 10.3.5 Binding of Secondary Antibody. 10.4 Determination of Cell Number -- 10.4.1 Required Material -- 10.4.2 Method -- 10.5 Immunofluorescence Assay -- 10.5.1 Principle -- 10.5.2 Immunofluorescence Technique -- 10.5.3 Labelling of Antibodies with Fluorochromes -- 10.5.4 Detection of Fluorochrome-Labelled Reagent -- 10.5.5 Selection of Fluorochrome -- 10.5.6 Materials -- 10.5.7 Blocking Buffer -- 10.5.8 Dilution Buffer -- 10.5.9 Fixative Solution -- 10.5.10 Immunostaining -- 10.5.11 Immunofluorescence Staining Method -- 10.5.12 Uses -- References -- 11: Advances in Microscopy -- 11.1 Introduction -- 11.2 Light Microscopy -- 11.2.1 Physical Properties of Light -- 11.2.2 Reflection -- 11.2.3 Transmission -- 11.2.4 Absorption -- 11.2.5 Refraction -- 11.2.6 Diffraction -- 11.2.7 The Human Eye -- 11.2.8 Polarization -- 11.2.9 Fluorescence -- 11.2.10 Important Concepts in Microscopy -- 11.2.11 Contrast -- 11.2.12 Magnification -- 11.2.13 Sensitivity -- 11.2.14 Simple Theory of Microscopy -- 11.2.15 Metric Units Used in Microscopy -- 11.2.16 Light Microscopes -- 11.2.16.1 The Compound Light Microscope -- 11.2.16.2 Inverted Microscope -- 11.3 Dark Field Microscope -- 11.4 Phase Contrast Microscopy -- 11.5 Differential Interference Contrast Microscopy (DIC) -- 11.6 Fluorescence Microscopy -- 11.6.1 Fluorescent Antibody Technique or Immunofluorescence -- 11.6.2 Identification of Chromosome -- 11.6.2.1 Fluorescence In Situ Hybridization (FISH) -- 11.7 Polarization Microscopy -- 11.8 Confocal Microscopy -- 11.9 Electron Microscopy -- 11.9.1 Introduction -- 11.9.2 Transmission Electron Microscope (TEM) -- 11.9.3 Scanning Electron Microscope -- 11.9.4 Scanning Tunneling Microscope (STM) -- 11.9.5 Atomic Force Microscope -- 11.9.6 Sample Preparation for Light Microscope -- 11.9.6.1 Wet Mount Method -- 11.9.7 Histological Techniques -- 11.9.8 Sample Preparation for Electron Microscope. 11.9.9 Sample Preparation of TEM and SEM. |
| Record Nr. | UNINA-9910561300603321 |
Mandal Santi M.
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| Gateway East, Singapore : , : Springer, , [2022] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Avoiding Questionable Research Practices in Applied Psychology [[electronic resource] /] / edited by William O'Donohue, Akihiko Masuda, Scott Lilienfeld
| Avoiding Questionable Research Practices in Applied Psychology [[electronic resource] /] / edited by William O'Donohue, Akihiko Masuda, Scott Lilienfeld |
| Edizione | [1st ed. 2022.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 |
| Descrizione fisica | 1 online resource (439 pages) |
| Disciplina | 615.5028 |
| Soggetto topico |
Psychometrics
Psychology Science - Philosophy Behavioral Sciences and Psychology Philosophy of Science Psicologia aplicada Investigació psicològica Metodologia de la ciència |
| Soggetto genere / forma | Llibres electrònics |
| ISBN | 3-031-04968-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | 1. What are Questionable Research Practices? -- 2. The Logic of Research and Questionable Research Methods -- 3. Heuristics and Biases, Intellectual Humility, and Research Design -- 4. History of Replication Failures in Psychology -- 5. P Hacking -- 6. Methods of Detecting QRPs, Such as p curve Analysis -- 7. Controversies Regarding Null Hypothesis Testing -- 8. HARKING and Confirmatory vs. Exploratory Analyses -- 9. History of Statistical Controversies -- 10. Publication Bias and Negative Results -- 11. Power Analysis and Underpowered Studies -- 12. Controversies in Single Subject Designs -- 13. Ignoring Problematic Psychometrics -- 14. Honest and Transparent Report Writing -- 15. Effect Size Measurement -- 16. Pre-registration Models -- 17. Adversarial Research -- 18. Open Data -- 19. Replication Project -- 20. Summary: Designing, Conducting, and Reporting Research. |
| Record Nr. | UNINA-9910590050903321 |
| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Experimental Landscape Ecology [[electronic resource] /] / by Yolanda F. Wiersma
| Experimental Landscape Ecology [[electronic resource] /] / by Yolanda F. Wiersma |
| Autore | Wiersma Yolanda F (Yolanda Francine), <1973-> |
| Edizione | [1st ed. 2022.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 |
| Descrizione fisica | 1 online resource (229 pages) |
| Disciplina | 574.5072 |
| Collana | Landscape Series |
| Soggetto topico |
Landscape ecology
Applied ecology Research - Methodology Ecology Ecologia del paisatge Investigació Metodologia de la ciència |
| Soggetto genere / forma | Llibres electrònics |
| ISBN |
9783030951894
9783030951887 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Part I. Introduction -- Part II. Philosophy and Concepts of Experimentation -- Part III. Approaches to Experimentation -- Part IV. Conclusion -- Part V. Resources. . |
| Record Nr. | UNINA-9910559385603321 |
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Wiersma Yolanda F (Yolanda Francine), <1973->
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| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Mad knowledges and user-led research / / Diana Susan Rose
| Mad knowledges and user-led research / / Diana Susan Rose |
| Autore | Rose Diana Susan |
| Edizione | [1st ed. 2022.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Palgrave Macmillan, , 2022 |
| Descrizione fisica | 1 online resource (336 pages) |
| Disciplina | 121 |
| Collana | The Politics of Mental Health and Illness |
| Soggetto topico |
Knowledge, Theory of
Psychiatry - Research Research - Methodology Teoria del coneixement Psiquiatria Investigació mèdica Metodologia de la ciència |
| Soggetto genere / forma | Llibres electrònics |
| ISBN |
9783031075513
9783031075506 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Part I Setting the Scene -- 1 What Does Madness Articulate? -- Background -- When Madness Begins to Speak What Does ‘It’ Say? -- Mobilising as Speaking Back? -- Non-uniformity -- Who Speaks? -- The Underground -- The Field -- The ‘Mainstreams’ of Survivor Voices—Psychiatry -- The Academy and ‘Psy’ Research -- Speaking Back to Austerity -- Conclusion -- References -- 2 Mental Challenges as Constitutive of Marginalisation? -- Background -- Collectives -- The Clinical Encounter -- Out of the Hospital -- Can Communities be Developed? -- Psychiatric Facilities and Policies as Obstacles to Forming Groups -- Survivors as a ‘Marginalised Community’ -- Counter-Narratives from the Mainstream -- Activism -- What Is to be Done? -- Conclusion -- References -- Part II User Involvement in Research—England as a Case Study -- 3 History of Patient and Public Involvement in England -- Background and Summary -- Language and Representation -- Who Is the Public? -- Can I Apply? -- What Is ‘Meaningful’ Involvement? -- Power -- Changes in Structures -- Research by ‘Lay’ People Outside Official Structures -- Hidden from PPI—Lay Research -- Conclusion -- References -- 4 Research and Practice or What About the Wild? -- The Problems -- Scope -- Impact: The Beginning -- The Question of ‘Impact’ in Involvement Activities -- What Is Meant by the Term ‘Impact’? Two Approaches -- Evidence-Based Medicine (EbM) -- Science and Technology Studies -- Survivor Research and Change -- The Fundamental Difference -- Where Does Madness Sit in the PPI Terrain? -- Back to ‘Impact’ -- What Happens When PPI Happens? -- The Secret of Process -- Process Evaluations -- Going Beyond PPI -- Conclusion -- References -- 5 Working with Others and ‘Coproduction’ -- Background -- Does Everybody Agree? -- Addressing the Field -- Relevant Themes -- Settings, Partners and Mental Health -- Historical Aspects of Coproduction -- Examples of Coproduction? -- The Costs of Coproduction? -- Partners and Allies -- Power Again -- Conclusion -- References -- Part III Foundational Categories and User-Led Research -- 6 Experience as a Foundation of Knowledge-Making: What’s in a Name? -- Background -- Process -- Naming -- Terminology, Identity and ‘Who Counts’ -- Lived Experience -- Disability Under Erasure -- From Soft to Strong and Back Again -- Survivordom -- A Third Way? -- Implications of Names -- Experts and How Should We Talk to Them? -- The Move to Lived Experience -- Activism and Knowledge-Making -- Conclusion -- References -- 7 Experience: What’s in a Foundational Category? -- Background -- Examples from the Literature -- Whose Experiences Does Research Need? -- Mental Health Specifically -- Diversity -- Lived Experience: What Is It? -- Liminal Identities in Liminal Spaces -- Individual and Collective Experience -- Conclusion: Experience in Context -- References -- 8 Specific Projects Led by Service Users -- Background -- Being a User/Survivor Researcher -- Example 1: Consumer/Patient-Centred Systematic Reviews -- Analysis from the Present -- Example 2: Participatory Research -- User-Generated Outcome Measures -- Outcome Measures -- VOICE -- Delving into the Conversations -- Participatory Research and Power -- Ethics -- Community Validation -- Modifying the Model -- Is There a ‘Community’ of Mental Health Service Users/Survivors? -- Conclusion -- References -- Part IV Guiding Principles -- 9 Intersectionality and Mental Life Disturbed -- Background -- Approach -- Women and Madness -- Practice -- Mad Politics -- Feminist Therapy and Structural Violence -- Racialisation and Madness in the West -- The Sharp End of Psychiatry -- A White Survivor Movement -- Problems -- Conclusion -- References -- 10 Conclusion -- Theory -- Conditions for Silencing Madness -- Mental Health and Meaning in the West -- War -- Trauma -- Not All Meaning Is Therapeutic -- If Madness Is Meaningful What Are Its Conditions? -- The Routledge International Handbook of Mad Studies -- The Social Model of Disability -- Psychosocial Disability -- Reasonable Accommodations -- What Is Madness? -- Activism and Last Words -- Joining up Conditions -- Implications for Research -- Activism as Knowledge—A Provocation -- Conclusion -- References -- Index. |
| Record Nr. | UNINA-9910595042203321 |
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Rose Diana Susan
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| Cham : , : Springer International Publishing : , : Imprint : Palgrave Macmillan, , 2022 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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