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Biophysical analysis of membrane proteins : investigating structure and function / / edited by Eva Pebay-Peyroula
| Biophysical analysis of membrane proteins : investigating structure and function / / edited by Eva Pebay-Peyroula |
| Pubbl/distr/stampa | Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2008 |
| Descrizione fisica | 1 online resource (369 p.) |
| Disciplina |
572.6
572/.69 |
| Soggetto topico |
Membrane proteins - Structure-activity relationships
Membrane proteins - Analysis Cell membranes - Physiology Physical biochemistry |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-282-78421-8
9786612784217 3-527-62122-9 3-527-62123-7 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | ger |
| Nota di contenuto |
Biophysical Analysis of Membrane Proteins; Contents; Preface; The Editor; List of Contributors; Part I Introduction; 1 High-Resolution Structures of Membrane Proteins: From X-Ray Crystallography to an Integrated Approach of Membranes; 1.1 Membranes: A Soft Medium?; 1.2 Current Knowledge on Membrane Protein Structures; 1.2.1 An Overview of the Protein Data Bank; 1.2.2 Protein Sources for Structural Studies; 1.2.3 The Diversity of Membrane Protein Topologies; 1.2.4 Genome Analyses; 1.3 X-Ray Crystallography; 1.3.1 Crystallization of Membrane Proteins; 1.3.2 General Aspects of Crystallography
1.3.3 Determining the Phases Associated with Diffracted Waves1.3.4 Structure Determination of Membrane Proteins; 1.3.4.1 Crystal Quality; 1.3.4.2 Phase Determination; 1.3.4.3 Crystal Freezing; 1.4 Recent Examples; 1.4.1 Bacterial Rhodopsins; 1.4.2 ADP/ATP Carrier; 1.4.3 Oligomerization of Membrane Proteins in their Natural Environment; 1.5 Future Developments in X-Ray Crystallography of Membrane Proteins; 1.6 Conclusions; Part II Structural Approaches; 2 Membrane Protein Structure Determination by Electron Cryo-Microscopy; 2.1 Introduction; 2.1.1 The Electron Microscope 2.2 Single-Particle Electron Microscopy2.2.1 Sample Preparation and Requirements; 2.2.1.1 Negative Staining of Specimens; 2.2.1.2 Cryo-EM of Unstained Specimens; 2.2.1.3 Choice of detergent; 2.2.2 Image Analysis; 2.2.2.1 Classification of Images; 2.2.2.2 Model Building and Refinement; 2.2.2.3 Assessing Resolution; 2.2.3 Future Perspectives; 2.3 Structure Determination from 2-Dimensional Crystals; 2.3.1 Two-Dimensional Crystallization of Membrane Proteins; 2.3.2 Image Acquisition and Structure Determination; 2.3.3 Future Perspectives; 2.4 Helical Analysis of Tubes; 2.5 Conclusions 3 Introduction to Solid-State NMR and its Application to Membrane Protein-Ligand Binding Studies3.1 Introduction; 3.1.1 Membrane Proteins: A Challenge; 3.1.2 Why Solid-State NMR?; 3.2 Solid-State NMR; 3.2.1 Sample Preparation: What is an Ideal Sample?; 3.2.1.1 Availability; 3.2.1.2 Stability; 3.2.1.3 Secondary Structure; 3.2.1.4 Sample Form: Local Order; 3.2.2 NMR Active Isotopes and Labeling; 3.2.3 Assignment and Structure Determination; 3.2.4 NMR Techniques: Solution- versus Solid-State NMR; 3.2.4.1 Isotropic Liquids; 3.2.4.2 Anisotropic Liquids; 3.2.4.3 Solids 3.3 Examples: Receptor-Ligand Studies by Solid-State NMR3.3.1 Transport Proteins; 3.3.1.1 LacS; 3.3.2 G-Protein-Coupled Receptors and Related Proteins; 3.3.2.1 Bacteriorhodopsin, Rhodopsin, and Sensory Rhodopsin (NpSRII); 3.3.2.2 Human H(1) Receptor; 3.3.2.3 Neurotensin Receptor; 3.3.3 Ion Channels; 3.3.3.1 Nicotinic Acetylcholine Receptor; 3.3.3.2 K(+) Ion Channel, KcsA; 3.3.4 P-type ATPases; 3.3.5 Membrane Protein Soluble Alternatives; Part III Molecular Interaction and Large Assemblies; 4 Analytical Ultracentrifugation: Membrane Protein Assemblies in the Presence of Detergent 4.1 Introduction |
| Record Nr. | UNINA-9910144732103321 |
| Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biophysical analysis of membrane proteins : investigating structure and function / / edited by Eva Pebay-Peyroula
| Biophysical analysis of membrane proteins : investigating structure and function / / edited by Eva Pebay-Peyroula |
| Pubbl/distr/stampa | Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2008 |
| Descrizione fisica | 1 online resource (369 p.) |
| Disciplina |
572.6
572/.69 |
| Soggetto topico |
Membrane proteins - Structure-activity relationships
Membrane proteins - Analysis Cell membranes - Physiology Physical biochemistry |
| ISBN |
1-282-78421-8
9786612784217 3-527-62122-9 3-527-62123-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | ger |
| Nota di contenuto |
Biophysical Analysis of Membrane Proteins; Contents; Preface; The Editor; List of Contributors; Part I Introduction; 1 High-Resolution Structures of Membrane Proteins: From X-Ray Crystallography to an Integrated Approach of Membranes; 1.1 Membranes: A Soft Medium?; 1.2 Current Knowledge on Membrane Protein Structures; 1.2.1 An Overview of the Protein Data Bank; 1.2.2 Protein Sources for Structural Studies; 1.2.3 The Diversity of Membrane Protein Topologies; 1.2.4 Genome Analyses; 1.3 X-Ray Crystallography; 1.3.1 Crystallization of Membrane Proteins; 1.3.2 General Aspects of Crystallography
1.3.3 Determining the Phases Associated with Diffracted Waves1.3.4 Structure Determination of Membrane Proteins; 1.3.4.1 Crystal Quality; 1.3.4.2 Phase Determination; 1.3.4.3 Crystal Freezing; 1.4 Recent Examples; 1.4.1 Bacterial Rhodopsins; 1.4.2 ADP/ATP Carrier; 1.4.3 Oligomerization of Membrane Proteins in their Natural Environment; 1.5 Future Developments in X-Ray Crystallography of Membrane Proteins; 1.6 Conclusions; Part II Structural Approaches; 2 Membrane Protein Structure Determination by Electron Cryo-Microscopy; 2.1 Introduction; 2.1.1 The Electron Microscope 2.2 Single-Particle Electron Microscopy2.2.1 Sample Preparation and Requirements; 2.2.1.1 Negative Staining of Specimens; 2.2.1.2 Cryo-EM of Unstained Specimens; 2.2.1.3 Choice of detergent; 2.2.2 Image Analysis; 2.2.2.1 Classification of Images; 2.2.2.2 Model Building and Refinement; 2.2.2.3 Assessing Resolution; 2.2.3 Future Perspectives; 2.3 Structure Determination from 2-Dimensional Crystals; 2.3.1 Two-Dimensional Crystallization of Membrane Proteins; 2.3.2 Image Acquisition and Structure Determination; 2.3.3 Future Perspectives; 2.4 Helical Analysis of Tubes; 2.5 Conclusions 3 Introduction to Solid-State NMR and its Application to Membrane Protein-Ligand Binding Studies3.1 Introduction; 3.1.1 Membrane Proteins: A Challenge; 3.1.2 Why Solid-State NMR?; 3.2 Solid-State NMR; 3.2.1 Sample Preparation: What is an Ideal Sample?; 3.2.1.1 Availability; 3.2.1.2 Stability; 3.2.1.3 Secondary Structure; 3.2.1.4 Sample Form: Local Order; 3.2.2 NMR Active Isotopes and Labeling; 3.2.3 Assignment and Structure Determination; 3.2.4 NMR Techniques: Solution- versus Solid-State NMR; 3.2.4.1 Isotropic Liquids; 3.2.4.2 Anisotropic Liquids; 3.2.4.3 Solids 3.3 Examples: Receptor-Ligand Studies by Solid-State NMR3.3.1 Transport Proteins; 3.3.1.1 LacS; 3.3.2 G-Protein-Coupled Receptors and Related Proteins; 3.3.2.1 Bacteriorhodopsin, Rhodopsin, and Sensory Rhodopsin (NpSRII); 3.3.2.2 Human H(1) Receptor; 3.3.2.3 Neurotensin Receptor; 3.3.3 Ion Channels; 3.3.3.1 Nicotinic Acetylcholine Receptor; 3.3.3.2 K(+) Ion Channel, KcsA; 3.3.4 P-type ATPases; 3.3.5 Membrane Protein Soluble Alternatives; Part III Molecular Interaction and Large Assemblies; 4 Analytical Ultracentrifugation: Membrane Protein Assemblies in the Presence of Detergent 4.1 Introduction |
| Record Nr. | UNINA-9910829991203321 |
| Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
