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Biomedical electron microscopy [[electronic resource] ] : illustrated methods and interpretations / / Arvid B. Maunsbach, Björn A. Afzelius
| Biomedical electron microscopy [[electronic resource] ] : illustrated methods and interpretations / / Arvid B. Maunsbach, Björn A. Afzelius |
| Autore | Maunsbach Arvid Bernhard |
| Pubbl/distr/stampa | San Diego, CA, : Academic Press, c1999 |
| Descrizione fisica | 1 online resource (569 p.) |
| Disciplina |
502.825
570.2825 570/.28/25 21 |
| Altri autori (Persone) | AfzeliusBjörn |
| Soggetto topico |
Electron microscopy - Technique
Medical microscopy - Technique |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-27948-X
9786611279486 0-08-052809-0 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; BIOMEDICAL ELECTRON MICROSCOPY; Copright Page; FOREWORD; PREFACE; ACKNOWLEDGMENTS; CHAPTER 1. MICROGRAPH INTERPRETATION; 1. Classical Preparation Method; 2. Low Temperature Approach; 3. A Common Test Specimen; 4. Detection of Objects; 5. Identification of Artifacts; 6. Analysis of Geometry; 7. Biological Identification; 8. Biological Diversity; 9. Analysis of Dynamics: Endocytosis; 10. Analysis of Dynamics: Synthesis; 11. Comparison of Methods; 12. Variations in Magnifications; 13. Interpretation Difficulties; 14. Diagnostic Pathology; CHAPTER 2. FIXATIVES
1. Osmium Tetroxide and Glutaraldehyde at Low Magnification2. Osmium Tetroxide and Glutaraldehyde at High Magnification; 3. Glutaraldehyde Concentration: Perfusion Fixation; 4. Glutaraldehyde Concentration: Immersion Fixation; 5. Long Fixation Times; 6. Formaldehyde-Glutaraldehyde Combinations; 7. Potassium Permanganate, Picric Acid, and Ruthenium Red; 8. Lead Salts and Tannic Acid; 9. Uranyl Acetate Postfixation; 10. Tannic Acid-Uranyl Acetate Variations; 11. Osmium Tetroxide-Potassium Ferrocyanide; 12. Osmium Tetroxide Artifacts; 13. Glutaraldehyde Artifacts; CHAPTER 3. FIXATIVE VEHICLE 1. Absence and Presence of Buffer2. Comparison of Buffers; 3. Osmolality of Perfusion Fixatives; 4. Effects of Osmolality on Cell Shape; 5. Effects of Osmolality on Cell Organelles; 6. Adjustment of Osmolality with Sucrose; 7. Colloid Osmotic Pressure: Low Magnification; 8. Colloid Osmotic Pressure: High Magnification; 9. Phosphate Buffer Precipitate; CHAPTER 4. FIXATIVE APPLICATION; 1. Perfusion-Fixation versus Immersion-Fixation; 2. Perfusion-Fixation with Pressure Control; 3. Fixation by Dripping in Vivo; 4. Immersion-Fixation; 5. Variability within the Tissue 6. Unsuccessful Perfusion-Fixation7. Superficial Tissue Damage; 8. Early Postmortal Changes; 9. Late Postmortal Changes; 10. Influence of Biopsy Method; 11. Microwave Treatment; CHAPTER 5. DEHYDRATION AND EMBEDDING; 1. Stepwise versus Direct Dehydration; 2. Prolonged Dehydration in Ethanol; 3. Prolonged Dehydration in Acetone; 4. Inert Dehydration; 5. Choice of Intermediate Solvent; 6. Epon, Araldite, and Vestopal: Unstained Sections; 7. Epon, Araldite, and Vestopal: Stained Sections; 8. Different Brands of Epoxy Resins; 9. Spurr and LR White; 10. Embedding of Isolated Cells CHAPTER 6. FREEZING AND LOW-TEMPERATURE EMBEDDING1. Plunge Freezing; 2. Contact Freezing of Unfixed Tissue; 3. Contact Freezing of Fixed Tissue; 4. High-Pressure Freezing; 5. Freeze-Substitution in Methanol/Uranyl Acetate; 6. Freeze-Substitution in Osmium Tetroxide Acetone; 7. Progressive Lowering of Temperature Embedding in Lowicryl; CHAPTER 7. SUPPORT FILMS; 1. Surface Topography; 2. Stability of Film or Section; 3. Holey Films; 4. Thick and Thin Support Films; 5. Folds in Support Film; 6. Defects in Formvar Films; 7. Common Contaminants; 8. Volatile Contamination; CHAPTER 8. ULTRAMICROTOMY 1. Correlation of Light and Electron Microscopy |
| Record Nr. | UNINA-9910457984003321 |
Maunsbach Arvid Bernhard
|
||
| San Diego, CA, : Academic Press, c1999 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biomedical electron microscopy [[electronic resource] ] : illustrated methods and interpretations / / Arvid B. Maunsbach, Björn A. Afzelius
| Biomedical electron microscopy [[electronic resource] ] : illustrated methods and interpretations / / Arvid B. Maunsbach, Björn A. Afzelius |
| Autore | Maunsbach Arvid Bernhard |
| Pubbl/distr/stampa | San Diego, CA, : Academic Press, c1999 |
| Descrizione fisica | 1 online resource (569 p.) |
| Disciplina |
502.825
570.2825 570/.28/25 21 |
| Altri autori (Persone) | AfzeliusBjörn |
| Soggetto topico |
Electron microscopy - Technique
Medical microscopy - Technique |
| ISBN |
1-281-27948-X
9786611279486 0-08-052809-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; BIOMEDICAL ELECTRON MICROSCOPY; Copright Page; FOREWORD; PREFACE; ACKNOWLEDGMENTS; CHAPTER 1. MICROGRAPH INTERPRETATION; 1. Classical Preparation Method; 2. Low Temperature Approach; 3. A Common Test Specimen; 4. Detection of Objects; 5. Identification of Artifacts; 6. Analysis of Geometry; 7. Biological Identification; 8. Biological Diversity; 9. Analysis of Dynamics: Endocytosis; 10. Analysis of Dynamics: Synthesis; 11. Comparison of Methods; 12. Variations in Magnifications; 13. Interpretation Difficulties; 14. Diagnostic Pathology; CHAPTER 2. FIXATIVES
1. Osmium Tetroxide and Glutaraldehyde at Low Magnification2. Osmium Tetroxide and Glutaraldehyde at High Magnification; 3. Glutaraldehyde Concentration: Perfusion Fixation; 4. Glutaraldehyde Concentration: Immersion Fixation; 5. Long Fixation Times; 6. Formaldehyde-Glutaraldehyde Combinations; 7. Potassium Permanganate, Picric Acid, and Ruthenium Red; 8. Lead Salts and Tannic Acid; 9. Uranyl Acetate Postfixation; 10. Tannic Acid-Uranyl Acetate Variations; 11. Osmium Tetroxide-Potassium Ferrocyanide; 12. Osmium Tetroxide Artifacts; 13. Glutaraldehyde Artifacts; CHAPTER 3. FIXATIVE VEHICLE 1. Absence and Presence of Buffer2. Comparison of Buffers; 3. Osmolality of Perfusion Fixatives; 4. Effects of Osmolality on Cell Shape; 5. Effects of Osmolality on Cell Organelles; 6. Adjustment of Osmolality with Sucrose; 7. Colloid Osmotic Pressure: Low Magnification; 8. Colloid Osmotic Pressure: High Magnification; 9. Phosphate Buffer Precipitate; CHAPTER 4. FIXATIVE APPLICATION; 1. Perfusion-Fixation versus Immersion-Fixation; 2. Perfusion-Fixation with Pressure Control; 3. Fixation by Dripping in Vivo; 4. Immersion-Fixation; 5. Variability within the Tissue 6. Unsuccessful Perfusion-Fixation7. Superficial Tissue Damage; 8. Early Postmortal Changes; 9. Late Postmortal Changes; 10. Influence of Biopsy Method; 11. Microwave Treatment; CHAPTER 5. DEHYDRATION AND EMBEDDING; 1. Stepwise versus Direct Dehydration; 2. Prolonged Dehydration in Ethanol; 3. Prolonged Dehydration in Acetone; 4. Inert Dehydration; 5. Choice of Intermediate Solvent; 6. Epon, Araldite, and Vestopal: Unstained Sections; 7. Epon, Araldite, and Vestopal: Stained Sections; 8. Different Brands of Epoxy Resins; 9. Spurr and LR White; 10. Embedding of Isolated Cells CHAPTER 6. FREEZING AND LOW-TEMPERATURE EMBEDDING1. Plunge Freezing; 2. Contact Freezing of Unfixed Tissue; 3. Contact Freezing of Fixed Tissue; 4. High-Pressure Freezing; 5. Freeze-Substitution in Methanol/Uranyl Acetate; 6. Freeze-Substitution in Osmium Tetroxide Acetone; 7. Progressive Lowering of Temperature Embedding in Lowicryl; CHAPTER 7. SUPPORT FILMS; 1. Surface Topography; 2. Stability of Film or Section; 3. Holey Films; 4. Thick and Thin Support Films; 5. Folds in Support Film; 6. Defects in Formvar Films; 7. Common Contaminants; 8. Volatile Contamination; CHAPTER 8. ULTRAMICROTOMY 1. Correlation of Light and Electron Microscopy |
| Record Nr. | UNINA-9910784528503321 |
|
Maunsbach Arvid Bernhard
|
||
| San Diego, CA, : Academic Press, c1999 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biomedical electron microscopy [[electronic resource] ] : illustrated methods and interpretations / / Arvid B. Maunsbach, Björn A. Afzelius
| Biomedical electron microscopy [[electronic resource] ] : illustrated methods and interpretations / / Arvid B. Maunsbach, Björn A. Afzelius |
| Autore | Maunsbach Arvid Bernhard |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | San Diego, CA, : Academic Press, c1999 |
| Descrizione fisica | 1 online resource (569 p.) |
| Disciplina |
502.825
570.2825 570/.28/25 21 |
| Altri autori (Persone) | AfzeliusBjörn |
| Soggetto topico |
Electron microscopy - Technique
Medical microscopy - Technique |
| ISBN |
1-281-27948-X
9786611279486 0-08-052809-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; BIOMEDICAL ELECTRON MICROSCOPY; Copright Page; FOREWORD; PREFACE; ACKNOWLEDGMENTS; CHAPTER 1. MICROGRAPH INTERPRETATION; 1. Classical Preparation Method; 2. Low Temperature Approach; 3. A Common Test Specimen; 4. Detection of Objects; 5. Identification of Artifacts; 6. Analysis of Geometry; 7. Biological Identification; 8. Biological Diversity; 9. Analysis of Dynamics: Endocytosis; 10. Analysis of Dynamics: Synthesis; 11. Comparison of Methods; 12. Variations in Magnifications; 13. Interpretation Difficulties; 14. Diagnostic Pathology; CHAPTER 2. FIXATIVES
1. Osmium Tetroxide and Glutaraldehyde at Low Magnification2. Osmium Tetroxide and Glutaraldehyde at High Magnification; 3. Glutaraldehyde Concentration: Perfusion Fixation; 4. Glutaraldehyde Concentration: Immersion Fixation; 5. Long Fixation Times; 6. Formaldehyde-Glutaraldehyde Combinations; 7. Potassium Permanganate, Picric Acid, and Ruthenium Red; 8. Lead Salts and Tannic Acid; 9. Uranyl Acetate Postfixation; 10. Tannic Acid-Uranyl Acetate Variations; 11. Osmium Tetroxide-Potassium Ferrocyanide; 12. Osmium Tetroxide Artifacts; 13. Glutaraldehyde Artifacts; CHAPTER 3. FIXATIVE VEHICLE 1. Absence and Presence of Buffer2. Comparison of Buffers; 3. Osmolality of Perfusion Fixatives; 4. Effects of Osmolality on Cell Shape; 5. Effects of Osmolality on Cell Organelles; 6. Adjustment of Osmolality with Sucrose; 7. Colloid Osmotic Pressure: Low Magnification; 8. Colloid Osmotic Pressure: High Magnification; 9. Phosphate Buffer Precipitate; CHAPTER 4. FIXATIVE APPLICATION; 1. Perfusion-Fixation versus Immersion-Fixation; 2. Perfusion-Fixation with Pressure Control; 3. Fixation by Dripping in Vivo; 4. Immersion-Fixation; 5. Variability within the Tissue 6. Unsuccessful Perfusion-Fixation7. Superficial Tissue Damage; 8. Early Postmortal Changes; 9. Late Postmortal Changes; 10. Influence of Biopsy Method; 11. Microwave Treatment; CHAPTER 5. DEHYDRATION AND EMBEDDING; 1. Stepwise versus Direct Dehydration; 2. Prolonged Dehydration in Ethanol; 3. Prolonged Dehydration in Acetone; 4. Inert Dehydration; 5. Choice of Intermediate Solvent; 6. Epon, Araldite, and Vestopal: Unstained Sections; 7. Epon, Araldite, and Vestopal: Stained Sections; 8. Different Brands of Epoxy Resins; 9. Spurr and LR White; 10. Embedding of Isolated Cells CHAPTER 6. FREEZING AND LOW-TEMPERATURE EMBEDDING1. Plunge Freezing; 2. Contact Freezing of Unfixed Tissue; 3. Contact Freezing of Fixed Tissue; 4. High-Pressure Freezing; 5. Freeze-Substitution in Methanol/Uranyl Acetate; 6. Freeze-Substitution in Osmium Tetroxide Acetone; 7. Progressive Lowering of Temperature Embedding in Lowicryl; CHAPTER 7. SUPPORT FILMS; 1. Surface Topography; 2. Stability of Film or Section; 3. Holey Films; 4. Thick and Thin Support Films; 5. Folds in Support Film; 6. Defects in Formvar Films; 7. Common Contaminants; 8. Volatile Contamination; CHAPTER 8. ULTRAMICROTOMY 1. Correlation of Light and Electron Microscopy |
| Record Nr. | UNINA-9910828688003321 |
|
Maunsbach Arvid Bernhard
|
||
| San Diego, CA, : Academic Press, c1999 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
