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Immunogenetics [[electronic resource] ] : Methods and Protocols / / edited by Anton W. Langerak
| Immunogenetics [[electronic resource] ] : Methods and Protocols / / edited by Anton W. Langerak |
| Autore | Langerak Anton W |
| Edizione | [1st ed. 2022.] |
| Pubbl/distr/stampa | New York, NY, : Springer Nature, 2022 |
| Descrizione fisica | 1 online resource (XVI, 607 p. 132 illus., 121 illus. in color.) |
| Disciplina |
571.9648
616.0796 |
| Collana | Methods in Molecular Biology |
| Soggetto topico |
Immunogenetics
Immunospecificity Adaptive Immunity Immunogenètica Receptors cel·lulars Manuals de laboratori |
| Soggetto genere / forma | Llibres electrònics |
| Soggetto non controllato |
The immunome
Adaptive immune cells Antigen receptors Immunoinformatics T-cell receptors Lymphocytes |
| ISBN | 1-0716-2115-7 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | The Advent of Precision Immunology: Immunogenetics at the Center of Immune Cell Analysis in Health and Disease -- Next-Generation Sequencing-Based Clonality Detection of Immunoglobulin Gene Rearrangements in B-Cell Lymphoma -- One-Step Next Generation Sequencing of Immunoglobulin and T-Cell Receptor Gene Recombinations for MRD Marker Identification in Acute Lymphoblastic Leukemia -- Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq -- Minimal Residual Disease Analysis by Monitoring Immunoglobulin and T-Cell Receptor Gene Rearrangements by Quantitative PCR and Droplet Digital PCR -- Quality Control for IG/TR Marker Identification and MRD Analysis -- cfDNA-Based NGS IG Analysis in Lymphoma -- Targeted Locus Amplification as Marker Screening Approach to Detect Immunoglobulin (IG) Translocations in B-Cell Non-Hodgkin Lymphomas -- Immunoglobulin/T-Cell Receptor Capture Strategy for Comprehensive Immunogenetics -- Immunoglobulin Gene Mutational Status Assessment by Next Generation Sequencing in Chronic Lymphocytic Leukemia -- NGS-Based B-Cell Receptor Repertoire Analysis in the Context of Inborn Errors of Immunity -- Generic Multiplex Digital PCR for Accurate Quantification of T-Cells in Copy Number Stable and Unstable DNA Samples -- Gene Engineering T-Cells with T-Cell Receptor for Adoptive Therapy -- Combined Analysis of Transcriptome and T-Cell Receptor Alpha Beta (TRA/TRB) Repertoire in Paucicellular Samples at the Single Cell Level -- AIRR Community Guide to Planning and Performing AIRR-Seq Experiments -- Adaptive Immune Receptor Repertoires (AIRR) Community Guide to TR and IG Gene Annotation -- Adaptive Immune Receptor Repertoires (AIRR) Community Guide to Repertoire Analysis -- Bulk gDNA Sequencing of Antibody Heavy Chain Gene Rearrangements for Detection and Analysis of B-Cell Clone Distribution: A Method by the AIRR Community -- Bulk Sequencing from mRNA with UMI for Evaluation of B-Cell Isotype and Clonal Evolution: A Method by the AIRR Community -- Single Cell Analysis and Tracking of Antigen-Specific T-Cells: Integrating Paired-Chain AIRR-Seq and Transcriptome Sequencing: A Method by the AIRR Community -- Quality Control: Chain Pairing Precision and Monitoring of Cross-Sample Contamination: A Method by the AIRR Community -- Immune Repertoire Analysis on High-Performance Computing Using VDJServer V1: A Method by the AIRR Community -- Data Sharing and Re-Use: A Method by the AIRR Community -- IMGT® Immunoinformatics Tools for Standardized V-DOMAIN Analysis -- IMGT/3Dstructure-DB: T-Cell Receptor TR Paratope and Peptide/Major Histocompatibility pMH Contact Sites and Epitope -- ARResT/Interrogate Immunoprofiling Platform: Concepts, Workflows, and Insights -- Purpose-Built Immunoinformatics for BcR IG/TR Repertoire Data Analysis. |
| Record Nr. | UNINA-9910576888603321 |
Langerak Anton W
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| New York, NY, : Springer Nature, 2022 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Immunology : overview and laboratory manual / / Tobili Y. Sam-Yellowe
| Immunology : overview and laboratory manual / / Tobili Y. Sam-Yellowe |
| Autore | Sam-Yellowe Tobili Y. |
| Edizione | [1st ed. 2021.] |
| Pubbl/distr/stampa | Cham, Switzerland : , : Springer, , [2021] |
| Descrizione fisica | 1 online resource (xx, 471 pages) : colour illustrations |
| Disciplina | 616.079 |
| Soggetto topico |
Immunology
Immunologia Manuals de laboratori |
| Soggetto genere / forma | Llibres electrònics |
| ISBN | 3-030-64686-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Part I: Overview of Immunity -- Ch 1: Immunity -- Ch 2: Components of immunity -- Ch 3: Hematopoiesis -- Ch 4: The Immune system: pathogen sensing and detection -- Ch 5: Lymphoid organs -- Ch 6: Innate and Adaptive immunity -- Ch 7: Leukocyte homing, migration and recirculation -- Ch 8: Apoptosis -- Ch 9: Antigens -- Ch 10: B cell development, activation and immunoglobulin structure -- Ch 11: Immunoglobulin and T cell receptor gene rearrangements -- Ch 12: Major histocompatibility complex proteins -- Ch 13: T cell development and T cell receptor structure -- Ch 14: Cytokines -- Ch 15: Lymphocyte signals -- Ch 16: Complement fixation -- Ch 17: Cell mediated immunity -- Ch 18: Hypersensitivities -- Ch 19: Parasite Immunity -- Ch 20: Vaccines -- Ch 21: Transplantation -- Ch 22: Immune regulation and autoimmunity -- Ch 23: Immunodeficiencies -- Ch 24: Cancer -- Ch 25: Practice questions -- Ch 26: Study guide for exam. Part II. Laboratory Manual --Ch 27. Immunology laboratory policies -- Ch 28. Instructions for notebook entries -- Ch 29. Instructions for writing lab reports -- Ch 30. Lab report topics -- Ch 31. Exercise 1: Using a compound microscope -- Ch 32. Exercise 2: Pipetting and dilution technique, identification of mouse lymphoid organs -- Ch 33. Exercise 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing -- Ch 34. Exercise 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens -- Ch 35. Exercise 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells -- Ch 36. Exercise 6: Clearance of bacteria from mouse blood by the RES -- Ch 37. Exercise 7: Hemolytic Plaque assay (The Jeme Plaque Assay) -- Ch 38. EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays -- Ch 39. EXERCISE 9: Determination of Antibody Titer: Screening Techniques -- Ch 40. EXERCISE 10: Immunodiffusion and Immunoelectrophoresis -- Ch 41. EXERCISE 11: Protein Affinity Purification of Rabbit IgG -- Ch 42. EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) -- Ch 43. EXERCISE 13: Immunoblotting (Western Blotting) -- Ch 44. Exercise 14: Protein Assay -- Ch 45. Exercise 15: Enzyme-linked Immunosorbent Assay (ELISA) -- Ch 46. EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT) -- Ch 47. EXERCISE 17: Immunofluorescence assay (IFA) -- Ch 48. EXERCISE 18: Flow Cytometry -- Ch 49.EXERCISE 19: Complement Fixation Assays -- Ch 50. Tissue Cell Culture Techniques -- Ch 51. EXERCISE 20: Mitogen Induced Response of Lymphocytes: Detection of Band T cell Lymphocytes Using Immunofluorescence -- Ch 52. Exercise 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells -- Ch 53. EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production -- Ch 54. EXERCISE 23: Macrophage Migration Inhibition -- Ch 55. EXERCISE 24: Monoclonal Antibody Preparation -- Ch 56. EXERCISE 25: Immunization of mice with a recombinant protein -- Ch 57. EXERCISE 26: Polarization of T helper cells into TH1 subset -- Ch 58. Exercise 27: Immunoprecipitation -- Ch 59. EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers -- Ch 60. EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies. |
| Record Nr. | UNINA-9910495233203321 |
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Sam-Yellowe Tobili Y.
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| Cham, Switzerland : , : Springer, , [2021] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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In Vitro Diagnostic Industry in China / / edited by Haibo Song, Jian'er Yao
| In Vitro Diagnostic Industry in China / / edited by Haibo Song, Jian'er Yao |
| Edizione | [1st ed. 2021.] |
| Pubbl/distr/stampa | Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2021 |
| Descrizione fisica | 1 online resource (IX, 299 p. 33 illus., 31 illus. in color.) |
| Disciplina | 616 |
| Soggetto topico |
Diseases
Oncology Medicine—Research Biology—Research Biomedical Research Manuals de laboratori Diagnòstic de laboratori |
| Soggetto genere / forma | Llibres electrònics |
| ISBN | 981-16-2316-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Policy and Environment -- Current Situation and Progress -- Opportunities and Challenges -- Opinions and Suggestions -- Chemiluminescence Immunoassay -- Introduction of Hot Technologies and Products for Blood Coagulation Detection -- Development and Prospect of Laboratory Automation -- Clinical Application and Prospect of Automatic Examination Technology -- Biochemical Diagnosis -- Immune Diagnosis -- Molecular Diagnosis -- Clinical Testing -- Microbiological Detection -- Point-of-care Testing -- Mergers and Acquisitions of China's IVD Industry -- Overseas Mergers and Acquisitions. |
| Record Nr. | UNINA-9910495216303321 |
| Singapore : , : Springer Nature Singapore : , : Imprint : Springer, , 2021 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Laboratory Protocols in Fungal Biology : Current Methods in Fungal Biology / / edited by Vijai Kumar Gupta, Maria Tuohy
| Laboratory Protocols in Fungal Biology : Current Methods in Fungal Biology / / edited by Vijai Kumar Gupta, Maria Tuohy |
| Edizione | [2nd ed. 2022.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 |
| Descrizione fisica | 1 online resource (265 pages) |
| Disciplina |
571.29
579.5078 |
| Collana | Fungal Biology |
| Soggetto topico |
Fungi
Mycology Microbiology Botanical chemistry Biotechnology Plants - Development Plant Biochemistry Plant Development Fongs Manuals de laboratori |
| Soggetto genere / forma | Llibres electrònics |
| ISBN | 3-030-83749-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Preface -- Long Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results -- Confocal Laser and Epifluorescence Microscopy -- Diagnose of Chytid Parasites of Algae in The Plankton -- Sabouraud Agar for Fungal Growth -- FISH, Fluorescence in Situ Hybridization of Uncultured Zoosporic Fungi -- Real-time Quantitative PCR Assay for Counting Uncultured Zoosporic Fungi -- Real-Time PCR Assay in Fungi -- Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes -- Extraction and Characterization of Taxol: An Anticancer Drug from an Endophytic And Pathogenic Fungi -- Recent Advances in Applications of Machine Learning in Fungal Biology -- Fungal Conservation -- TBD -- Loop-Mediated Isothermal Amplification of the Entomopathogenic Fungi, Beauveria bassiana -- Molecular Taxonomy and Multi-Gene Phylogeny of Fungi -- Yeast Isolation Methods from Specialized Habitats -- Assays for the Quantification of Antioxidant Enzymes in Fungi -- Index. |
| Record Nr. | UNINA-9910522565203321 |
| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Microscopic Techniques for the Non-Expert / / edited by Sathish-Kumar Kamaraj, Arun Thirumurugan, Shanmuga Sundar Dhanabalan, Samuel A. Hevia
| Microscopic Techniques for the Non-Expert / / edited by Sathish-Kumar Kamaraj, Arun Thirumurugan, Shanmuga Sundar Dhanabalan, Samuel A. Hevia |
| Edizione | [1st ed. 2022.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 |
| Descrizione fisica | 1 online resource (251 pages) |
| Disciplina | 578 |
| Soggetto topico |
Medicine—Research
Biology—Research Materials—Microscopy Crystallography Nanotechnology Materials—Analysis Imaging systems Biomedical Research Microscopy Crystallography and Scattering Methods Characterization and Analytical Technique Imaging Techniques Microscòpia Manuals de laboratori |
| Soggetto genere / forma | Llibres electrònics |
| ISBN | 3-030-99542-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | A Beginner’s Guide to Different Types of Microscopes -- Principles of Light and Fluorescence Microscopy -- Confocal and Multiphoton Microscopy -- Scanning Probe Microscopy: tipping the path towards atomic visions -- Atomic Force Microscopy - An Advanced Imaging Technique: From Molecules to Morphologies -- Exploring the microcosm at atomic precision using Atomic Force Microscopy -- Scanning Electron Microscopy (SEM): Learning to generate and interpret the topographical aspects of materials -- Recent updates on methods, applications, and practical uses of scanning electron microscopy in various life sciences -- Transmission electron microscopy - A powerful and novel scientific technique with nanoscale resolution for characterization of materials -- Preparation of Biological Samples for SEM: Techniques and Procedures -- Index. |
| Record Nr. | UNINA-9910735397703321 |
| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2022 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Models and techniques in stroke biology / / Amit Kumar Tripathi, Abhishek Kumar Singh, editors
| Models and techniques in stroke biology / / Amit Kumar Tripathi, Abhishek Kumar Singh, editors |
| Pubbl/distr/stampa | Singapore : , : Springer, , [2021] |
| Descrizione fisica | 1 online resource (vii, 115 pages) : illustrations |
| Disciplina | 574.028 |
| Soggetto topico |
Biology - Technique
Malalties cerebrovasculars Models biològics Manuals de laboratori |
| Soggetto genere / forma | Llibres electrònics |
| ISBN | 981-336-679-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Intro -- Contents -- About the Editors -- Chapter 1: Rodent Stroke Model Guidelines: An Update -- 1.1 Introduction -- 1.2 The General Guideline for a Rodent Model for Stroke -- 1.3 STAIR Committee, Recommendations, and Guidelines -- 1.4 Intraluminal Model -- 1.5 Different Types of the Occluder and their Success Rate -- 1.6 PLL-Coated Occluders -- 1.7 Flame Blunted Occluders -- 1.8 Silicone-Rubber Coated Occluders -- 1.9 An Optimization Technique for Intraluminal Inserting Occluders -- 1.10 Optimization of Surgical Procedure -- 1.11 The Tamura Method of a Stroke Model -- 1.12 Infarction Area Estimation -- 1.13 Methods for Infarction Visualization -- 1.14 Direct Image of Infarction in Fresh Brain Sections Stained with TTC Solution -- 1.15 Digital Method for Defining the Infarction -- 1.16 Calculation of Infarction Volume -- 1.17 Preparation of Stroke Surgical Procedure -- 1.18 Koizumi´s Suture Method -- 1.19 Longa´s MCAO Method -- 1.20 Neurobehaviour Assessment of Functional Recovery -- 1.21 Assessment of Neurological Functional Outcomes -- 1.21.1 Composite Score -- 1.21.1.1 Bederson Scale and Neurological Deficit Scoring -- 1.21.1.2 Modified Neurological Severity Score -- 1.21.2 Motor Tests -- 1.21.2.1 Cylinder Test -- 1.21.2.2 Ledged Tapered Beam Test -- 1.21.2.3 Pellet Retrieval Task -- 1.21.3 Sensorimotor Test -- 1.21.3.1 Forelimb Flexion -- 1.21.3.2 Forelimb Placing -- 1.21.3.3 Accelerated Rotarod Test -- 1.21.3.4 Adhesive Removal Test -- 1.22 Anesthetics -- 1.23 Monitoring and Maintaining the Core Brain Temperature -- 1.24 Mechanical Ventilation, Blood Gases, Glucose, and Blood Pressure Monitoring -- 1.25 Pilot Study Plan and Implementation of a Preclinical Stroke Trial -- 1.26 Application of the Suitable Statistical Method for Data Analysis -- 1.27 Standard Operating Procedures for Creating a Stroke Model.
1.28 Conclusion and Future Direction -- References -- Chapter 2: Bilateral Common Carotid Artery Occlusion: Stroke Model -- 2.1 Introduction -- 2.2 Importance of BCCAO -- 2.3 Permanent BCCAO -- 2.4 Transient BCCAO -- 2.5 BCCAO Sham Surgery -- 2.6 Factors Affecting the BCCAO -- 2.6.1 Effect of Ischemic Preconditioning -- 2.7 Effect of ROS -- 2.8 Effect of Mitochondrial Dysfunction -- 2.9 Role of the Apoptotic Pathway -- 2.10 Effect of BCCAO Duration and Animal Model Sex -- 2.11 Effect on Blood-Brain Barrier -- 2.12 Effect of Oxygen Free Radicals -- 2.13 Conclusion -- References -- Chapter 3: Cerebral Venous Sinus Thrombosis Rodent Model -- 3.1 Introduction -- 3.2 Clinical Manifestation, Diagnosis, and Prognosis of CVST -- 3.3 Clinical Recommendation for Management of CVST Patients -- 3.4 Experimental CVST Model Protocol -- 3.5 Precautions -- 3.6 TTC Staining Technique -- 3.7 Neurological Evaluation -- 3.8 CVST as a Preclinical Model for Evaluation of Neuroprotective Agents -- References -- Chapter 4: A Non-human Primate Model for Cerebral Stroke -- 4.1 Introduction -- 4.2 Ischemia Model in NHPs -- 4.3 Major Advantages of NHPs Used as Stroke Model -- 4.4 Ethical Challenges -- 4.5 Permanent MCA Occlusion -- 4.6 Transient MCA Occlusion -- 4.7 Thrombus MCA Occlusion in NHPs -- References -- Chapter 5: Laser Doppler Flowmetry Recording for Rodent Stroke Model Confirmation -- 5.1 Introduction -- 5.2 Laser Doppler Flowmetry -- 5.3 Theory and Basic Principal Laser Doppler Flowmetry -- 5.4 Experimental Applications of Laser Doppler Flowmetry (LDF) -- 5.5 Advantages and Limitations of Laser Doppler Flow Meters -- 5.6 Conclusion -- References -- Chapter 6: Laser Speckle Imaging for Cerebral Ischemia and Reperfusion Injury -- 6.1 Introduction -- 6.2 Basic Principles of Laser Speckle Contrast Imaging -- 6.3 Application in Clinical and Biomedical Research. 6.3.1 Preclinical Application of LSCI in the Rodent Model of Cerebral Ischemic Stroke -- 6.3.2 Applications of LSCI in Stroke Induced Language Impairment -- 6.4 Limitations of the LSCI Technique -- 6.5 Conclusion and Recommendations -- References -- Chapter 7: Cerebrovascular Imaging in a Rodent Stroke Model -- 7.1 Introduction -- 7.2 Cerebrovascular Imaging -- 7.3 Cerebral Vessels Staining with a Combination of Carbon Black Inks -- 7.4 Assessment of Cerebral Vascular Network -- 7.5 Alternative Methods for Cerebral Vascular Anatomy -- 7.6 Current Challenges and Future Prospective -- References -- Chapter 8: Photothrombotic Stroke Model -- 8.1 Introduction -- 8.2 Induction of Photothrombotic Lesion -- 8.2.1 Surgery for Illumination of the Region of Interest -- 8.2.2 Injection and Activation of Rose Bengal -- 8.3 Assessment of Photothrombotic Lesion -- 8.4 Merits and Demerits of the Photothrombotic Stroke Model -- 8.5 Conclusion -- References. |
| Record Nr. | UNINA-9910484098603321 |
| Singapore : , : Springer, , [2021] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Molecular mechanisms in spermatogenesis / / C. Yan Cheng, Fei Sun, editors
| Molecular mechanisms in spermatogenesis / / C. Yan Cheng, Fei Sun, editors |
| Edizione | [Second edition.] |
| Pubbl/distr/stampa | Cham, Switzerland : , : Springer, , [2022] |
| Descrizione fisica | 1 online resource (322 pages) |
| Disciplina | 612.61 |
| Collana | Advances in experimental medicine and biology |
| Soggetto topico |
Spermatogenesis
Espermatogènesi Testicle Biologia molecular Manuals de laboratori |
| Soggetto genere / forma | Llibres electrònics |
| ISBN | 3-030-77779-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910495172703321 |
| Cham, Switzerland : , : Springer, , [2022] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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