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Manual of Molecular and Clinical Laboratory Immunology : 2 Volume Set
| Manual of Molecular and Clinical Laboratory Immunology : 2 Volume Set |
| Autore | Schmitz John L |
| Edizione | [9th ed.] |
| Pubbl/distr/stampa | Newark : , : ASM Press, , 2024 |
| Descrizione fisica | 1 online resource (3222 pages) |
| Disciplina | 616.07/9 |
| Altri autori (Persone) |
DetrickBarbara
O'GormanMaurice R. G |
| Collana | ASM Bks. |
| Soggetto topico |
Immunologic Techniques
Immunologic Tests - methods |
| Soggetto genere / forma | Laboratory Manual |
| ISBN |
9781683674023
1683674022 9781683674016 1683674014 9781683674009 1683674006 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover -- Volume 1 -- Title Page -- Copyright Page -- Contents -- Editorial Board -- Contributors -- Preface -- Author and Editor Conflicts of interest -- Section A Molecular Methods -- Chapter 1 Introduction -- References -- Chapter 2 Molecular Methods for Genetic Diagnosis of Inborn Errors of Immunity -- Introduction -- Sequencing Technique Progression -- Sanger Sequencing -- High-Throughput Sequencing -- Pre- and postsequencing Considerations -- Sample Selection -- Analysis of variants -- Additional Techniques -- Copy Number Variation -- Trecs -- Droplet Digital PCR -- Future Considerations -- Acknowledgments -- References -- Chapter 3 Immunoglobulin and T-cell Receptor Gene Assessment -- Introduction -- Protein Structure of immunoglobulin -- Basic Immunoglobulin Structure -- Heavy-chain Isotypes -- Structure of the B-cell Receptor -- Protein Structure of the T-cell Receptor (tcr) -- T- and B-cell Development -- T-cell Development -- B Cell Development -- Genetic Structure of Immunoglobulins and t-cell Receptor -- Immunoglobulin Genes -- T-cell Receptor Genes -- Immunoglobulin and t-cell Receptor Gene Rearrangement -- B- and T-cell Receptor Gene Rearrangement Mechanism -- CDR3 Diversity -- J (joining) Region -- C (constant) Region -- Recombination Signal Sequences -- IGHC Class Switching -- Somatic Hypermutation -- Productive/unproductive Rearrangement -- Molecular Methods for TCR and IG Rearrangement Testing -- PCR-based Assays -- NGS-based Assays -- Considerations In assay Design -- Quality Controls for NGS Clonality Assays -- Interpretation of TCR and IG Rearrangement Testing -- Clonality Diagnostics and MRD Testing -- MRD Diagnostics Using Cell-free DNA -- Conclusions -- References -- Section B Immunoglobulin Methods -- Chapter 4 Introduction -- References.
Chapter 5 Clinical Indications and applications of Serum and Urine Protein Electrophoresis -- Background on protein Structure -- Principles of Protein Electrophoresis -- Zone Electrophoresis -- Quality Control and quality Assurance -- Specimen Requirements for Serum -- Specimen Requirements for Urine -- Internal Controls -- Proteins Identified In Serum Protein Electrophoresis -- Pattern Interpretation In Serum -- Liver Disease Pattern -- Protein Loss Patterns -- Acute-phase Reaction -- Detection of M-proteins In Serum -- Quantification of Serum M-proteins -- Pattern Interpretation In Urine -- Detection of M-Proteins In the Urine -- Clinical Applications -- Avoiding False-positive Results -- Correlation of current With previous Patient Results -- References -- Chapter 6 Immunochemical Characterization of immunoglobulins In Serum, Urine, and cerebrospinal Fluid -- Background -- Laboratory Investigation -- Immunofixation -- Immunosubtraction -- Pattern Interpretations and clinical Disorders -- Polyclonal -- Endogenous Monoclonal Proteins -- Monoclonal Gammopathies -- Diseases With monoclonal Immunoglobulin Deposition -- Heavy Chain Diseases -- Plasma Cell Neoplasms -- Exogenous (therapeutic) Monoclonal Proteins and chimeric Proteins -- Oligoclonal Banding -- Hypogammaglobulinemia -- Artifacts -- Quality Control -- Urine Immunofixation -- Cerebrospinal Fluid -- Laboratory Investigation -- References -- Chapter 7 Cryoglobulins and Cryofibrinogenemia -- Cryoglobulins -- Background -- Concept -- Procedures -- Isolation, Quantitation, and characterization of cryoglobulins -- Assays for specific Antibody Activities, Antigens, or Detection of nucleic Acid -- Complement Measurements In cryoglobulinemia -- Conclusions -- Intralaboratory Quality Control and interlaboratory Proficiency Testing -- Cryofibrinogens -- Background -- Concept -- Interpretation. Procedures -- Conclusions -- Anticoagulant-related Recommendations -- References -- Chapter 8 Strategy for detecting and following Monoclonal Gammopathies -- Classification of monoclonal Gammopathies -- Malignant Plasma Cell Proliferative Disorders -- Premalignant Plasma Cell Proliferative Diseases -- Protein (or Low-tumor-burden) Diseases -- Overview of laboratory Tests for monoclonal Gammopathies -- Diagnostic Testing Strategies for M-Proteins In symptomatic Patients -- Monitoring M-proteins -- Stratification of risk -- Summary -- References -- Section C Complement -- Chapter 9 Introduction -- References -- Chapter 10 Methods of complement Analysis -- Introduction -- Preanalytical Considerations -- Diagnostic Workup -- Methods of modern Complement Analysis -- Functional Assays -- Protein Quantification of individual Components -- Analysis of complement Activation Products -- Multiplex Analysis of complement Proteins -- Monitoring of complement-targeted Drugs -- Complement Analysis In tissues -- Quality Assessment -- Outlook -- References -- Chapter 11 Assessing Complement Dysregulation In the Thrombotic Microangiopathies and ultrarare Complement-mediated Kidney Disease -- Alternative Pathway of complement -- Complement-mediated Renal Disease -- Atypical Hemolytic Uremic Syndrome -- C3 Glomerulopathy -- Conclusions -- Acknowledgements -- References -- Chapter 12 Current and future of complement Therapeutics and their Impact On the Clinical Immunology Laboratory -- C1-Inihibitor Concentrates -- Anti-C5 Antibodies -- Eculizumab and ABP959 -- Ravulizumab -- Crovalimab -- Pozelimab -- Anti-C5a Antibody -- Anti-masp-2 Antibody -- Cyclic Peptide C3 inhibitor -- Small-molecule C5a Receptor Antagonist -- Anti-C1 Antibodies -- Oral Small-molecule Inhibitors -- Pegylated RNA Aptamers -- RNA Interference -- Adeno-associated Virus (AAV) Gene therapy. References -- Section D Flow Cytometry -- Chapter 13 Introduction: Clinical Flow Cytometry Applications and quality Control -- References -- Chapter 14 Polychromatic Flow Cytometry -- Technology -- Instrumentation Overview -- Lasers -- Optical Filters -- Signal Processing and configuration of instrument Detectors -- Flow Cytometer Standardization -- Sample Preparation -- Sample Types and requirements -- Reagents -- Procedures -- Panel Design, One Approach -- Doublet Exclusion -- Quality Assurance and quality Control -- Requirements for validation of an Assay Include: -- Data Analysis and interpretation -- Future Directions -- References -- Chapter 15 High-sensitivity Detection of Paroxysmal Nocturnal Hemoglobinuria by Multiparameter Flow Cytometry -- Introduction -- Clinical Features of pnh -- Who Should Be tested for pnh? -- Complement Blockade Therapy and disease Monitoring In pnh -- Early Methods To test for pnh -- Evolution of flow Cytometric Methods To detect Pnh -- Issues With early Flow Methods -- Routine Versus High-sensitivity Flow Cytometry -- High-sensitivity Assays for pnh Red and white Blood Cells -- General Requirements -- High-sensitivity 2-c Assay for pnh In mature Rbcs -- Antibody Clone/conjugate Selection for High-sensitivity Detection of pnh MRBCs -- Instrument Setup Considerations for MRBCs -- MRBC Staining Protocol Using CD235a FITC/CD59 Pe Cocktail -- Issues With MRBC Assays -- Assays for pnh Within Immature Rbcs -- Cross-platform 3-color (3-c) Assay for pnh Irbcs and MRBCs -- Validation of 3-color Irbc/MRBC Assay To screen Pnh Samples -- High-sensitivity 4-Color (4-C) WBC assays -- Antibody Clone/conjugate Selection for 4-C High-sensitivity Pnh WBC Detection -- Instrument Setup Considerations: WBCs -- WBC Staining Procedure for optimized Neutrophil and monocyte Cocktails -- High-sensitivity 5-Color (5-C) WBC assays. Antibody Clone/conjugate Selection for 5-C High-sensitivity Pnh WBC Detection -- Instrument Setup Considerations for 5-Color WBC assay -- Staining Procedure for 5-Color High-sensitivity Combined Neutrophil/monocyte Assay -- Presence of type Ii Populations In neutrophils and monocytes -- CD157-negative Cases -- High-sensitivity 6-Color (6-C) WBC assays -- Antibody Clone/conjugate Selection for 6-C High-sensitivity Pnh WBC Detection -- IS FLAER Essential for high-sensitivity Testing of pnh WBCs? -- Cross-platform 5-, 6-, and 7-color Single Tube Assays for pnh WBCs -- Considerations for verification of high-sensitivity Pnh Assays -- Verification of instrument Setup and antibody Performance -- Internal Controls To verify Instrument Setup and antibody Conjugate Performance -- Fluorescence-minus-two (fm2) Controls To verify Instrument Settings -- Routine Control of assay Quality -- Considerations for validation of high-sensitivity Pnh Assays -- Ongoing QC and qc Monitors -- Reporting -- References -- Chapter 16 Standardized Flow Cytometry Assays for Enumerating CD34+ Hematopoietic Stem Cells -- Introduction -- Hematopoietic Stem/progenitor Cells Express CD34 Antigen -- Transplantation of hematopoietic Stem Cells -- Measuring Graft Adequacy -- Graft Assessment by CD34+ Cell Enumeration and Flow Cytometry -- Clinical Issues In enumerating Stem Cells by Flow Cytometry -- Early Methods to Measure CD34+ Cells by Flow Cytometry -- Statistical Issues In rare Event Detection -- Appropriate Controls for rare Event Detection -- Technical Issues in CD34+ Cell Enumeration by Multiparameter Flow Cytometry -- CD34 Antigen: Structural Considerations and choice of fluorochromes -- CD45 Antigen: Structural Considerations and choice of fluorochromes -- CD34+ Cell Enumeration Using Sequential Boolean Gating -- The Basic Ishage Protocol and gating Strategy. Single-platform Absolute CD34+ Cell Counting. |
| Record Nr. | UNINA-9911019317303321 |
Schmitz John L
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| Newark : , : ASM Press, , 2024 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Manual of molecular and clinical laboratory immunology / / edited by Barbara Detrick, John L. Schmitz, and Robert G. Hamilton
| Manual of molecular and clinical laboratory immunology / / edited by Barbara Detrick, John L. Schmitz, and Robert G. Hamilton |
| Edizione | [8th ed.] |
| Pubbl/distr/stampa | Washington, District of Columbia : , : ASM Press, , 2016 |
| Descrizione fisica | 1 online resource (1269 p.) |
| Disciplina | 616.079 |
| Soggetto topico |
Immunodiagnosis
Molecular immunology Immunology Immunologic Techniques Immunologic Tests - methods |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-68367-326-3
1-68367-077-9 1-55581-872-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910555160103321 |
| Washington, District of Columbia : , : ASM Press, , 2016 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Manual of molecular and clinical laboratory immunology / / edited by Barbara Detrick, John L. Schmitz, and Robert G. Hamilton
| Manual of molecular and clinical laboratory immunology / / edited by Barbara Detrick, John L. Schmitz, and Robert G. Hamilton |
| Edizione | [8th ed.] |
| Pubbl/distr/stampa | Washington, District of Columbia : , : ASM Press, , 2016 |
| Descrizione fisica | 1 online resource (1269 p.) |
| Disciplina | 616.079 |
| Soggetto topico |
Immunodiagnosis
Molecular immunology Immunology Immunologic Techniques Immunologic Tests - methods |
| ISBN |
1-68367-326-3
1-68367-077-9 1-55581-872-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910830290303321 |
| Washington, District of Columbia : , : ASM Press, , 2016 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
