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HPLC made to measure [[electronic resource] ] : a practical handbook for optimization / / edited by Stavros Kromidas
| HPLC made to measure [[electronic resource] ] : a practical handbook for optimization / / edited by Stavros Kromidas |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (789 p.) |
| Disciplina |
543.0894
543.84 |
| Altri autori (Persone) | KromidasStavros |
| Soggetto topico |
High performance liquid chromatography - Methodology
Liquid chromatography |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-31169-3
9786611311698 3-527-61197-5 3-527-61199-1 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
HPLC Made to Measure; Foreword; Preface; Contents; List of Contributors; Structure of the Book; 1 Fundamentals of Optimization; 1.1 Principles of the Optimization of HPLC Illustrated by RP-Chromatography; 1.1.1 Before the First Steps of Optimization; 1.1.2 What Exactly Do We Mean By "Optimization"?; 1.1.3 Improvement of Resolution ("Separate Better"); 1.1.3.1 Principal Possibilities for Improving Resolution; 1.1.3.2 What has the Greatest Effect on Resolution?; 1.1.3.3 Which Sequence of Steps is Most Logical When Attempting an Optimization?; 1.1.3.4 How to Change k, α, and N
1.1.3.4.1 Isocratic Mode1.1.3.4.2 Gradient Mode; 1.1.3.4.3 Acetonitrile or Methanol?; 1.1.4 Testing of the Peak Homogeneity; 1.1.5 Unknown Samples: "How Can I Start?"; Strategies and Concepts; 1.1.5.1 The "Two Days Method"; 1.1.5.2 "The 5-Step Model"; 1.1.6 Shortening of the Run Time ("Faster Separation"); 1.1.7 Improvement of the Sensitivity ("To See More", i.e. Lowering of the Detection Limit); 1.1.8 Economics in HPLC ("Cheaper Separation"); 1.1.9 Final Remarks and Outlook; References; 1.2 Fast Gradient Separations; 1.2.1 Introduction; 1.2.2 Main Part; 1.2.2.1 Theory; 1.2.2.2 Results 1.2.2.2.1 General Relationships1.2.2.2.2 Short Columns, Small Particles; 1.2.2.2.3 An Actual Example; 1.2.2.3 Optimal Operating Conditions and Limits of Currently Available Technology; 1.2.2.4 Problems and Solutions; 1.2.2.4.1 Gradient Delay Volume; 1.2.2.4.2 Detector Sampling Rate and Time Constant; 1.2.2.4.3 Ion Suppression in Mass Spectrometry; References; 1.3 pH and Selectivity in RP-Chromatography; 1.3.1 Introduction; 1.3.2 Main Section; 1.3.2.1 Ionization and pH; 1.3.2.2 Mobile Phase and pH; 1.3.2.2.1 Buffer Capacity 1.3.2.2.2 Changes of pK and pH Value in the Presence of an Organic Solvent1.3.2.3 Buffers; 1.3.2.3.1 Classical HPLC Buffers; 1.3.2.3.2 MS-Compatible pH Control; 1.3.2.4 Influence of the Samples; 1.3.2.4.1 The Sample Type: Acids, Bases, Zwitterions; 1.3.2.4.2 Influence of the Organic Solvent on the Ionization of the Analytes; 1.3.3 Application Example; 1.3.4 Troubleshooting; 1.3.4.1 Reproducibility Problems; 1.3.4.2 Buffer Strength and Solubility; 1.3.4.3 Constant Buffer Concentration; 1.3.5 Summary; References; 1.4 Selecting the Correct pH Value for HPLC; 1.4.1 Introduction 1.4.2 Typical Approaches to pH Selection1.4.3 Initial pH Selection; 1.4.4 Basis of pK(a) Prediction; 1.4.5 Correction of pH Based on Organic Content; 1.4.6 Optimization of Mobile Phase pH Without Chemical Structures; 1.4.7 A Systematic Approach to pH Selection; 1.4.8 An Example - Separation of 1,4-Bis[(2-pyridin-2-ylethyl)thio]butane-2,3-diol from its Impurities; 1.4.9 Troubleshooting Mobile Phase pH; 1.4.10 The Future; 1.4.11 Conclusion; References; 1.5 Optimization of the Evaluation in Chromatography; 1.5.1 Evaluation of Chromatographic Data - An Introduction; 1.5.2 Working Range 1.5.3 Internal Standard |
| Record Nr. | UNINA-9910146269003321 |
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
HPLC made to measure [[electronic resource] ] : a practical handbook for optimization / / edited by Stavros Kromidas
| HPLC made to measure [[electronic resource] ] : a practical handbook for optimization / / edited by Stavros Kromidas |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (789 p.) |
| Disciplina |
543.0894
543.84 |
| Altri autori (Persone) | KromidasStavros |
| Soggetto topico |
High performance liquid chromatography - Methodology
Liquid chromatography |
| ISBN |
1-281-31169-3
9786611311698 3-527-61197-5 3-527-61199-1 9783527313778 9783527611973 9783527611997 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
HPLC Made to Measure; Foreword; Preface; Contents; List of Contributors; Structure of the Book; 1 Fundamentals of Optimization; 1.1 Principles of the Optimization of HPLC Illustrated by RP-Chromatography; 1.1.1 Before the First Steps of Optimization; 1.1.2 What Exactly Do We Mean By "Optimization"?; 1.1.3 Improvement of Resolution ("Separate Better"); 1.1.3.1 Principal Possibilities for Improving Resolution; 1.1.3.2 What has the Greatest Effect on Resolution?; 1.1.3.3 Which Sequence of Steps is Most Logical When Attempting an Optimization?; 1.1.3.4 How to Change k, α, and N
1.1.3.4.1 Isocratic Mode1.1.3.4.2 Gradient Mode; 1.1.3.4.3 Acetonitrile or Methanol?; 1.1.4 Testing of the Peak Homogeneity; 1.1.5 Unknown Samples: "How Can I Start?"; Strategies and Concepts; 1.1.5.1 The "Two Days Method"; 1.1.5.2 "The 5-Step Model"; 1.1.6 Shortening of the Run Time ("Faster Separation"); 1.1.7 Improvement of the Sensitivity ("To See More", i.e. Lowering of the Detection Limit); 1.1.8 Economics in HPLC ("Cheaper Separation"); 1.1.9 Final Remarks and Outlook; References; 1.2 Fast Gradient Separations; 1.2.1 Introduction; 1.2.2 Main Part; 1.2.2.1 Theory; 1.2.2.2 Results 1.2.2.2.1 General Relationships1.2.2.2.2 Short Columns, Small Particles; 1.2.2.2.3 An Actual Example; 1.2.2.3 Optimal Operating Conditions and Limits of Currently Available Technology; 1.2.2.4 Problems and Solutions; 1.2.2.4.1 Gradient Delay Volume; 1.2.2.4.2 Detector Sampling Rate and Time Constant; 1.2.2.4.3 Ion Suppression in Mass Spectrometry; References; 1.3 pH and Selectivity in RP-Chromatography; 1.3.1 Introduction; 1.3.2 Main Section; 1.3.2.1 Ionization and pH; 1.3.2.2 Mobile Phase and pH; 1.3.2.2.1 Buffer Capacity 1.3.2.2.2 Changes of pK and pH Value in the Presence of an Organic Solvent1.3.2.3 Buffers; 1.3.2.3.1 Classical HPLC Buffers; 1.3.2.3.2 MS-Compatible pH Control; 1.3.2.4 Influence of the Samples; 1.3.2.4.1 The Sample Type: Acids, Bases, Zwitterions; 1.3.2.4.2 Influence of the Organic Solvent on the Ionization of the Analytes; 1.3.3 Application Example; 1.3.4 Troubleshooting; 1.3.4.1 Reproducibility Problems; 1.3.4.2 Buffer Strength and Solubility; 1.3.4.3 Constant Buffer Concentration; 1.3.5 Summary; References; 1.4 Selecting the Correct pH Value for HPLC; 1.4.1 Introduction 1.4.2 Typical Approaches to pH Selection1.4.3 Initial pH Selection; 1.4.4 Basis of pK(a) Prediction; 1.4.5 Correction of pH Based on Organic Content; 1.4.6 Optimization of Mobile Phase pH Without Chemical Structures; 1.4.7 A Systematic Approach to pH Selection; 1.4.8 An Example - Separation of 1,4-Bis[(2-pyridin-2-ylethyl)thio]butane-2,3-diol from its Impurities; 1.4.9 Troubleshooting Mobile Phase pH; 1.4.10 The Future; 1.4.11 Conclusion; References; 1.5 Optimization of the Evaluation in Chromatography; 1.5.1 Evaluation of Chromatographic Data - An Introduction; 1.5.2 Working Range 1.5.3 Internal Standard |
| Record Nr. | UNINA-9910830685003321 |
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
HPLC made to measure : a practical handbook for optimization / / edited by Stavros Kromidas
| HPLC made to measure : a practical handbook for optimization / / edited by Stavros Kromidas |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (789 p.) |
| Disciplina |
543.0894
543.84 |
| Altri autori (Persone) | KromidasStavros |
| Soggetto topico |
High performance liquid chromatography - Methodology
Liquid chromatography |
| ISBN |
9786611311698
9783527313778 9781281311696 1281311693 9783527611973 3527611975 9783527611997 3527611991 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
HPLC Made to Measure; Foreword; Preface; Contents; List of Contributors; Structure of the Book; 1 Fundamentals of Optimization; 1.1 Principles of the Optimization of HPLC Illustrated by RP-Chromatography; 1.1.1 Before the First Steps of Optimization; 1.1.2 What Exactly Do We Mean By "Optimization"?; 1.1.3 Improvement of Resolution ("Separate Better"); 1.1.3.1 Principal Possibilities for Improving Resolution; 1.1.3.2 What has the Greatest Effect on Resolution?; 1.1.3.3 Which Sequence of Steps is Most Logical When Attempting an Optimization?; 1.1.3.4 How to Change k, α, and N
1.1.3.4.1 Isocratic Mode1.1.3.4.2 Gradient Mode; 1.1.3.4.3 Acetonitrile or Methanol?; 1.1.4 Testing of the Peak Homogeneity; 1.1.5 Unknown Samples: "How Can I Start?"; Strategies and Concepts; 1.1.5.1 The "Two Days Method"; 1.1.5.2 "The 5-Step Model"; 1.1.6 Shortening of the Run Time ("Faster Separation"); 1.1.7 Improvement of the Sensitivity ("To See More", i.e. Lowering of the Detection Limit); 1.1.8 Economics in HPLC ("Cheaper Separation"); 1.1.9 Final Remarks and Outlook; References; 1.2 Fast Gradient Separations; 1.2.1 Introduction; 1.2.2 Main Part; 1.2.2.1 Theory; 1.2.2.2 Results 1.2.2.2.1 General Relationships1.2.2.2.2 Short Columns, Small Particles; 1.2.2.2.3 An Actual Example; 1.2.2.3 Optimal Operating Conditions and Limits of Currently Available Technology; 1.2.2.4 Problems and Solutions; 1.2.2.4.1 Gradient Delay Volume; 1.2.2.4.2 Detector Sampling Rate and Time Constant; 1.2.2.4.3 Ion Suppression in Mass Spectrometry; References; 1.3 pH and Selectivity in RP-Chromatography; 1.3.1 Introduction; 1.3.2 Main Section; 1.3.2.1 Ionization and pH; 1.3.2.2 Mobile Phase and pH; 1.3.2.2.1 Buffer Capacity 1.3.2.2.2 Changes of pK and pH Value in the Presence of an Organic Solvent1.3.2.3 Buffers; 1.3.2.3.1 Classical HPLC Buffers; 1.3.2.3.2 MS-Compatible pH Control; 1.3.2.4 Influence of the Samples; 1.3.2.4.1 The Sample Type: Acids, Bases, Zwitterions; 1.3.2.4.2 Influence of the Organic Solvent on the Ionization of the Analytes; 1.3.3 Application Example; 1.3.4 Troubleshooting; 1.3.4.1 Reproducibility Problems; 1.3.4.2 Buffer Strength and Solubility; 1.3.4.3 Constant Buffer Concentration; 1.3.5 Summary; References; 1.4 Selecting the Correct pH Value for HPLC; 1.4.1 Introduction 1.4.2 Typical Approaches to pH Selection1.4.3 Initial pH Selection; 1.4.4 Basis of pK(a) Prediction; 1.4.5 Correction of pH Based on Organic Content; 1.4.6 Optimization of Mobile Phase pH Without Chemical Structures; 1.4.7 A Systematic Approach to pH Selection; 1.4.8 An Example - Separation of 1,4-Bis[(2-pyridin-2-ylethyl)thio]butane-2,3-diol from its Impurities; 1.4.9 Troubleshooting Mobile Phase pH; 1.4.10 The Future; 1.4.11 Conclusion; References; 1.5 Optimization of the Evaluation in Chromatography; 1.5.1 Evaluation of Chromatographic Data - An Introduction; 1.5.2 Working Range 1.5.3 Internal Standard |
| Record Nr. | UNINA-9911019591903321 |
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Practical HPLC method development / / Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch
| Practical HPLC method development / / Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch |
| Autore | Snyder Lloyd R. |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : John Wiley & Sons, Inc., , 1997 |
| Descrizione fisica | 1 online resource (xxvi, 765 p. ) : ill |
| Disciplina | 543.0894 |
| Soggetto topico | High performance liquid chromatography - Methodology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
0-585-30111-5
0-471-67265-3 1-118-59201-8 1-118-59151-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910208836803321 |
Snyder Lloyd R.
|
||
| Hoboken, New Jersey : , : John Wiley & Sons, Inc., , 1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Practical HPLC method development / / Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch
| Practical HPLC method development / / Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch |
| Autore | Snyder Lloyd R. |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : John Wiley & Sons, Inc., , 1997 |
| Descrizione fisica | 1 online resource (xxvi, 765 p. ) : ill |
| Disciplina | 543.0894 |
| Soggetto topico | High performance liquid chromatography - Methodology |
| ISBN |
0-585-30111-5
0-471-67265-3 1-118-59201-8 1-118-59151-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910830367203321 |
|
Snyder Lloyd R.
|
||
| Hoboken, New Jersey : , : John Wiley & Sons, Inc., , 1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Selection of the HPLC method in chemical analysis / / Serban Moldoveanu, Victor David
| Selection of the HPLC method in chemical analysis / / Serban Moldoveanu, Victor David |
| Autore | Moldoveanu Șerban |
| Edizione | [First edition.] |
| Pubbl/distr/stampa | Waltham, MA : , : Elsevier, , [2017] |
| Descrizione fisica | 1 online resource (600 pages) : illustrations |
| Disciplina | 543/.84 |
| Soggetto topico | High performance liquid chromatography - Methodology |
| ISBN | 0-12-803711-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Start of the implementation of a new HPLC method -- Short overviews of analystical techniques not containing an independent separation step -- Short overviews of the main analytical techniques containing a separation step -- Basic information regarding HPLC techniques -- Properties of analytes and matrices determining HPLC selection -- General aspects regarding the HPLC analytical column -- RP-HPLC analytical columns -- Polar analytical columns -- Stationary phase and columns for ion exchange, ion-moderated, and ligand exchange chromatography -- Stationary phases and columns for chiral chromatography -- Stationary phases and columns for size exclusion -- Solvents, buffers, and additives used in the mobile phase -- Gradient elution -- The practice of HPLC. |
| Record Nr. | UNINA-9910149207003321 |
|
Moldoveanu Șerban
|
||
| Waltham, MA : , : Elsevier, , [2017] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
