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Fluorescence Probes for Sensing / / Sheshanath Vishwanath Bhosale
| Fluorescence Probes for Sensing / / Sheshanath Vishwanath Bhosale |
| Autore | Bhosale Sheshanath Vishwanath |
| Pubbl/distr/stampa | Basel : , : MDPI, , 2018 |
| Descrizione fisica | 1 online resource (vii, 303 pages) |
| Disciplina | 610/.28 |
| Soggetto topico |
Biosensors
Fluorescent probes Fluorescence |
| ISBN |
9783038429289
3038429287 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910765751603321 |
Bhosale Sheshanath Vishwanath
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| Basel : , : MDPI, , 2018 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescent and luminescent probes for biological activity [[electronic resource] ] : a practical guide to technology for quantitative real-time analysis / / edited by WT Mason
| Fluorescent and luminescent probes for biological activity [[electronic resource] ] : a practical guide to technology for quantitative real-time analysis / / edited by WT Mason |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | San Diego, Calif., : Academic Press, c1999 |
| Descrizione fisica | 1 online resource (697 p.) |
| Disciplina | 570/.28 |
| Altri autori (Persone) | MasonW. T |
| Collana | Biological techniques |
| Soggetto topico |
Fluorescent probes
Fluorescence |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-05700-2
9786611057008 0-08-053177-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide to Technology for Quantitative Real-Time Analysis; Copyright Page; Series Preface; Preface; Contributors; Contents; Part I: Introduction to Fluorescence Microscopy; Chapter One. Fluorescence Microscopy; 1.1 Introduction; 1.2 Microscope design; 1.3 Types of illumination; 1.4 Light sources; 1.5 Filters; 1.6 Objectives and eyepieces; References; Part II: Optical Probes and Their Applications; Chapter Two. Introduction to Fluorescent Probes: Properties, History and Applications; 2.1 Introduction
2.2 Nature of fluorescence and properties of fluorescent probes2.3 Historical developments; 2.4 Applications of fluorochromes in histology and microbiology; 2.5 Introduction of acridine orange into cell physiology, cytology and cytochemistry; 2.6 General applications of fluorescent probes; Acknowledgements; References; Chapter Three. Intracellular Ion Indicators; 3.1 Introduction; 3.2 General properties of intracellular ion indicators; 3.3 Examples of intracellular ion indicators; 3.4 Conclusions; Acknowledgements; References Chapter Four. Fluorescent Imaging of Nucleic Acids and Proteins in Gels4.1 Introduction; 4.2 General properties of fluorescent nucleic acid stains; 4.3 Examples of fluorescent nucleic acid gel stains; 4.4 General properties of fluorophore labels used to detect nucleic acids; 4.5 General properties of fluorescent protein gel stains; 4.6 Examples of fluorescent protein gel stains; 4.7 Protein labelling; 4.8 Conclusions; Acknowledgements; References; Part III: Using Optical Probes in Cells - Practicalities, Problems and Pitfalls Chapter Five. Introducing and Calibrating Fluorescent Probes in Cells and Organelles5.1 Introduction; 5.2 General principles of the loading process; 5.3 General principles of the calibration process; 5.4 Putting principles into practice; Acknowledgements; References; Chapter Six. Electroporation: A Method for Introduction of Non-permeable Molecular Probes; 6.1 Introduction; 6.2 Basic concept of electroporation; 6.3 Electric field generation and monitoring; 6.4 Polarization of the outer membrane; 6.5 Electropore formation and resealing; 6.6 Transmembrane transport 6.7 Practical considerations of electroporation6.8 Experimental evidence; 6.9 Summary; Acknowledgements; References; Chapter Seven. Imaging Reality: Understanding Maps of Physiological Cell Signals Measured by Fluorescence Microscopy and Digital Imaging; 7.1 Introduction; 7.2 Generic considerations for the use of fluorescent indicators; 7.3 Optimization of fluorescent light detection and background light correction; 7.4 3-D spatial maps of fluorescent signals; Acknowledgements; References; Chapter Eight. Fluorescent Probes in Practice - Potential Artifacts; 8.1 Introduction 8.2 Photobleaching |
| Record Nr. | UNINA-9910458576203321 |
| San Diego, Calif., : Academic Press, c1999 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Fluorescent and luminescent probes for biological activity [[electronic resource] ] : a practical guide to technology for quantitative real-time analysis / / edited by WT Mason
| Fluorescent and luminescent probes for biological activity [[electronic resource] ] : a practical guide to technology for quantitative real-time analysis / / edited by WT Mason |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | San Diego, Calif., : Academic Press, c1999 |
| Descrizione fisica | 1 online resource (697 p.) |
| Disciplina | 570/.28 |
| Altri autori (Persone) | MasonW. T |
| Collana | Biological techniques |
| Soggetto topico |
Fluorescent probes
Fluorescence |
| ISBN |
1-281-05700-2
9786611057008 0-08-053177-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide to Technology for Quantitative Real-Time Analysis; Copyright Page; Series Preface; Preface; Contributors; Contents; Part I: Introduction to Fluorescence Microscopy; Chapter One. Fluorescence Microscopy; 1.1 Introduction; 1.2 Microscope design; 1.3 Types of illumination; 1.4 Light sources; 1.5 Filters; 1.6 Objectives and eyepieces; References; Part II: Optical Probes and Their Applications; Chapter Two. Introduction to Fluorescent Probes: Properties, History and Applications; 2.1 Introduction
2.2 Nature of fluorescence and properties of fluorescent probes2.3 Historical developments; 2.4 Applications of fluorochromes in histology and microbiology; 2.5 Introduction of acridine orange into cell physiology, cytology and cytochemistry; 2.6 General applications of fluorescent probes; Acknowledgements; References; Chapter Three. Intracellular Ion Indicators; 3.1 Introduction; 3.2 General properties of intracellular ion indicators; 3.3 Examples of intracellular ion indicators; 3.4 Conclusions; Acknowledgements; References Chapter Four. Fluorescent Imaging of Nucleic Acids and Proteins in Gels4.1 Introduction; 4.2 General properties of fluorescent nucleic acid stains; 4.3 Examples of fluorescent nucleic acid gel stains; 4.4 General properties of fluorophore labels used to detect nucleic acids; 4.5 General properties of fluorescent protein gel stains; 4.6 Examples of fluorescent protein gel stains; 4.7 Protein labelling; 4.8 Conclusions; Acknowledgements; References; Part III: Using Optical Probes in Cells - Practicalities, Problems and Pitfalls Chapter Five. Introducing and Calibrating Fluorescent Probes in Cells and Organelles5.1 Introduction; 5.2 General principles of the loading process; 5.3 General principles of the calibration process; 5.4 Putting principles into practice; Acknowledgements; References; Chapter Six. Electroporation: A Method for Introduction of Non-permeable Molecular Probes; 6.1 Introduction; 6.2 Basic concept of electroporation; 6.3 Electric field generation and monitoring; 6.4 Polarization of the outer membrane; 6.5 Electropore formation and resealing; 6.6 Transmembrane transport 6.7 Practical considerations of electroporation6.8 Experimental evidence; 6.9 Summary; Acknowledgements; References; Chapter Seven. Imaging Reality: Understanding Maps of Physiological Cell Signals Measured by Fluorescence Microscopy and Digital Imaging; 7.1 Introduction; 7.2 Generic considerations for the use of fluorescent indicators; 7.3 Optimization of fluorescent light detection and background light correction; 7.4 3-D spatial maps of fluorescent signals; Acknowledgements; References; Chapter Eight. Fluorescent Probes in Practice - Potential Artifacts; 8.1 Introduction 8.2 Photobleaching |
| Record Nr. | UNINA-9910784529303321 |
| San Diego, Calif., : Academic Press, c1999 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescent dye labels and stains : a database of photophysical properties / / Tarso B. Ledur Kist
| Fluorescent dye labels and stains : a database of photophysical properties / / Tarso B. Ledur Kist |
| Autore | Ledur Kist Tarso B. <1962-> |
| Pubbl/distr/stampa | Hoboken, NJ : , : John Wiley & Sons, Inc., , [2023] |
| Descrizione fisica | 1 online resource (498 pages) |
| Disciplina | 570.282 |
| Soggetto topico |
Fluorescent labeling
Fluorescent probes Fluorescence microscopy - Technique |
| ISBN |
1-119-83516-X
1-119-83514-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910831190803321 |
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Ledur Kist Tarso B. <1962->
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| Hoboken, NJ : , : John Wiley & Sons, Inc., , [2023] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescent probes / edited by G. S. Beddard, M. A. West
| Fluorescent probes / edited by G. S. Beddard, M. A. West |
| Autore | Beddard, G. S. |
| Pubbl/distr/stampa | London : Academic Press, 1981 |
| Descrizione fisica | x, 235 p. : ill. ; 24 cm. |
| Disciplina | 571.64 |
| Altri autori (Persone) | West, Michael A. |
| Soggetto topico |
Biology - Technique
Cell membranes Cytology - Technique Fluorescent probes |
| ISBN | 0120846802 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991003475819707536 |
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Beddard, G. S.
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| London : Academic Press, 1981 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
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Handbook of fluorescent dyes and probes / / R.W. Sabnis
| Handbook of fluorescent dyes and probes / / R.W. Sabnis |
| Autore | Sabnis R. W (Ram Wasudeo) |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : Wiley, , 2015 |
| Descrizione fisica | 1 online resource (467 p.) |
| Disciplina | 667/.2 |
| Soggetto topico |
Optical brighteners
Dyes and dyeing Stains and staining Dyes and dyeing - Chemistry Fluorescent probes |
| ISBN |
1-119-00710-0
1-119-00709-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Title Page; Copyright; Dedication; Contents; Preface; About the Author; Chapter 1 Acridine Homodimer; References; Chapter 2 Acridine Orange (AO); References; Chapter 3 Acridine Orange 10-Dodecyl Bromide (Dodecyl-Acridine Orange (DAO)); References; Chapter 4 Acridine Orange 10-Nonyl Bromide (Nonyl-Acridine Orange (NAO)); References; Chapter 5 Alexa Fluor 350 Carboxylic Acid Succinimidyl Ester (AMCA-S); References; Chapter 6 Alexa Fluor 430 Carboxylic Acid Succinimidyl Ester; References; Chapter 7 Alexa Fluor 488 Carboxylic Acid Succinimidyl Ester; References
Chapter 8 Alexa Fluor 514 Carboxylic Acid Succinimidyl EsterReferences; Chapter 9 Alexa Fluor 532 Carboxylic Acid Succinimidyl Ester; References; Chapter 10 Alexa Fluor 555 Carboxylic Acid Succinimidyl Ester; References; Chapter 11 Alexa Fluor 594 Carboxylic Acid Succinimidyl Ester; References; Chapter 12 Alexa Fluor 633 Carboxylic Acid Succinimidyl Ester; References; Chapter 13 Alexa Fluor 660 Carboxylic Acid Succinimidyl Ester; References; Chapter 14 Alexa Fluor 680 Carboxylic Acid Succinimidyl Ester; References; Chapter 15 Alexa Fluor 700 Carboxylic Acid Succinimidyl Ester; References Chapter 16 Alexa Fluor 750 Carboxylic Acid Succinimidyl EsterReferences; Chapter 17 Alexa Fluor 790 Carboxylic Acid Succinimidyl Ester; References; Chapter 18 9-Amino-6-Chloro-2-Methoxyacridine (ACMA); References; Chapter 19 4-Amino-5-Methylamino-2',7'-Difluorofluorescein (DAFFM); References; Chapter 20 4-Amino-5-Methylamino-2',7'-Difluorofluorescein Diacetate (DAF-FM DA); References; Chapter 21 7-Amino-4-Methylcoumarin (AMC) (Coumarin 120) (Coumarin 440); References; Chapter 22 8-Aminopyrene-1,3,6-Trisulfonic Acid Trisodium Salt (APTS); References Chapter 23 7-Anilinocoumarin-4-Acetic Acid (ACAA)References; Chapter 24 9-Anthroylnitrile; References; Chapter 25 BOBO 1; References; Chapter 26 BOBO 3; References; Chapter 27 BODIPY FL C5-Ceramide (C5-DMB-ceramide); References; Chapter 28 BODIPY FL C5-Ganglioside GM1; References; Chapter 29 BODIPY FL C5-Lactosylceramide; References; Chapter 30 BODIPY FL C5-Sphingomyelin (C5-DMB-Sphingomyelin); References; Chapter 31 BODIPY FL C5-Succinimidyl Ester; References; Chapter 32 BODIPY TR Ceramide; References; Chapter 33 BO-PRO 1; References; Chapter 34 BO-PRO 3; References Chapter 35 4-Bromomethyl-6,7-DimethoxycoumarinReferences; Chapter 36 5-(Bromomethyl)Fluorescein; References; Chapter 37 4-Bromomethyl-7-Methoxycoumarin; References; Chapter 38 Calcein Blue AM; References; Chapter 39 5-Carboxy-2',7'-Dichlorofluorescein; References; Chapter 40 6-Carboxy-2',7'-Dichlorofluorescein; References; Chapter 41 5-Carboxy-2',7'-Dichlorofluorescein Diacetate; References; Chapter 42 6-Carboxy-2',7'-Dichlorofluorescein Diacetate; References; Chapter 43 5-Carboxyfluorescein; References; Chapter 44 6-Carboxyfluorescein; References; Chapter 45 5-Carboxyfluorescein Diacetate References |
| Record Nr. | UNINA-9910131264603321 |
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Sabnis R. W (Ram Wasudeo)
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| Hoboken, New Jersey : , : Wiley, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Intracellular Thermometry with Fluorescent Molecular Thermometers
| Intracellular Thermometry with Fluorescent Molecular Thermometers |
| Autore | Uchiyama Seiichi |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Newark : , : John Wiley & Sons, Incorporated, , 2024 |
| Descrizione fisica | 1 online resource (348 pages) |
| Disciplina | 536.50287 |
| Soggetto topico |
Fluorescent probes
Temperature measurements |
| ISBN |
9783527836840
3527836845 9783527836864 3527836861 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Intro -- Intracellular Thermometry with Fluorescent Molecular Thermometers -- Preface -- Contents -- 1 Temperature for Living Things -- 1.1 Temperature of Individuals -- 1.2 Responses to Temperature Variation at the Cellular Level -- 1.3 Significance of Intracellular Thermometry -- References -- 2 Fluorescent Molecular Thermometers -- 2.1 The Basics of Fluorescence -- 2.2 Responses of Fluorescent Molecular Thermometers -- 2.3 Small Organic Molecules Involving Intersystem Crossing -- 2.4 Small Organic Molecules with a Rotating Substituent Group -- 2.5 Reactive Small Organic Molecules -- 2.6 Viscosity-Sensitive Small Organic Molecules -- 2.7 Other Small Organic Molecules -- 2.8 Organometallic Complexes -- 2.9 Excimers and Exciplexes -- 2.10 Host-guest Interactions -- 2.11 Synthetic Polymers -- 2.12 Nucleic Acids (DNA and RNA) -- 2.13 Peptides -- 2.14 Fluorescent Proteins -- 2.15 Non-covalent Systems Based on Thermo-responsive Self-assembly and an Environment-sensitive Fluorophore -- 2.16 Inorganic Nanomaterials -- 2.17 Hybrid Nanomaterials -- References -- 3 Intracellular Thermometry with Fluorescent Molecular Thermometers -- 3.1 Early Attempts for Cellular Thermometry -- 3.2 Introduction of Fluorescent Molecular Thermometers into a Living Cell -- 3.3 Cytotoxicity Assessment -- 3.4 Practice of Intracellular Thermometry with Fluorescent Molecular Thermometers -- 3.5 Intracellular Thermometry with Organelle-targeted Fluorescent Molecular Thermometers -- 3.6 Intracellular Thermometry of Brown Adipocytes -- 3.7 Application of Intracellular Thermometry in Various Biological Fields -- References -- 4 Cellular Thermometry Based on Non-fluorometric Principles -- 4.1 Infrared Thermometry -- 4.2 Photoacoustic Thermometry -- 4.3 Raman Thermometry -- 4.4 Use of Transmission Spectroscopy -- 4.5 Thermocouple -- 4.6 Resonant Thermal Sensor.
4.7 Bimaterial Microcantilever -- 4.8 Thermistor -- References -- 5 Reliability Issue in Intracellular Thermometry -- 5.1 Sensitivity and Temperature Resolution -- 5.2 Functional Independency of Fluorescent Molecular Thermometers -- 5.3 Preparation of a Calibration Curve of Fluorescent Molecular Thermometers -- 5.4 Objection to Endogenous Thermogenesis -- 5.5 Possible Artifacts in Near-infrared Luminescent Thermometry and Proposal for Reliable Thermometry -- References -- 6 Applications of Intracellular Thermometry -- 6.1 Creation of New Biological Concepts -- 6.2 In vivo Temperature Measurements -- 6.3 Thermal Medicine at a Single-cell Level -- 6.4 Utilization of Nanoheater/Fluorescent Thermometer Hybrids in Biotechnology -- 6.5 Accurate Measurements of Absolute Intracellular Temperature -- 6.6 Simultaneous Monitoring of Intracellular Temperature and the Concentration of a Chemical Species Related to a Temperature Variation Inside a Living Cell -- 6.7 Intracellular Thermometry of Plant Cells -- References -- Appendix 1 Review and Feature Articles on Fluorescent Molecular Thermometers and Intracellular Thermometry in General -- Appendix 2 Comprehensive Collection of Fluorescent Polymeric Thermometers Based on the Combination of a Thermoresponsive Polymer and an Environment-sensitive Fluorophore -- Appendix 3 Comprehensive Collection of Fluorescent Nanogel Thermometers Based on the Combination of a Thermo-responsive Polymer and an Environment-sensitive Fluorophore -- Appendix 4 Comprehensive Collection of Non-covalent Fluorescent Temperature Sensing Systems Based on the Combination of a Thermo-responsive Polymer and an Environment-sensitive Fluorescent Compound -- Appendix 5 Comprehensive Collection of Fluorescent Polymeric Logic Gates Based on the Combination of a Thermo-responsive Polymer and an Environment-sensitive Fluorophore. Appendix 6 Solid Inorganic Nanostructures Showing Temperaturedependent Emission Properties -- Appendix 7 Tips for Intracellular Thermometry with Fluorescent Polymeric Thermometers Developed in the Author's Laboratory -- Index -- EULA. |
| Record Nr. | UNINA-9911020210403321 |
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Uchiyama Seiichi
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| Newark : , : John Wiley & Sons, Incorporated, , 2024 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Molecular fluorescent sensors for cellular studies / / edited by Elizabeth J. New
| Molecular fluorescent sensors for cellular studies / / edited by Elizabeth J. New |
| Pubbl/distr/stampa | Chichester, West Sussex : , : John Wiley & Sons, Incorporated, , 2022 |
| Descrizione fisica | 1 online resource (307 pages) |
| Soggetto topico |
Fluorescent probes
Biosensors Fluorescence spectroscopy |
| ISBN |
9781119749844
1-119-74984-0 1-119-74980-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover -- Title Page -- Copyright Page -- Contents -- List of Contributors -- Chapter 1 An Introduction to Small Molecule Fluorescent Sensors -- 1.1 What is Fluorescence? -- 1.2 Why Is Fluorescence Useful? -- 1.3 What Is a Fluorescent Sensor? -- 1.4 General Types of Fluorescent Sensors -- 1.5 Important Parameters -- 1.5.1 Excitation Maxima -- 1.5.2 Emission Maxima -- 1.5.3 Stokes Shift -- 1.5.4 Quantum Yield -- 1.5.5 Molar Extinction Coefficient -- 1.5.6 Brightness -- 1.5.7 Lifetime -- 1.5.8 Photobleaching -- 1.5.9 Anisotropy -- 1.5.10 Quenching -- 1.6 Fluorescence Mechanisms Used in Fluorescent Sensors -- 1.6.1 Photoinduced Electron Transfer -- 1.6.2 Internal Charge Transfer -- 1.6.3 Förster Resonance Energy Transfer -- 1.6.4 Through Bond Energy Transfer -- 1.6.5 Excited-State Intramolecular Proton Transfer -- 1.6.6 Aggregation-Induced Emission -- 1.6.7 Excimer Formation -- 1.7 Commonly Used Fluorophores -- 1.7.1 Fluorescein -- 1.7.2 Rhodamine -- 1.7.3 Coumarin -- 1.7.4 Naphthalimide -- 1.7.5 BODIPY (4,4-Difluoro4-bora-3a,4a-diaza-s-indacene) -- 1.7.6 Cyanine -- 1.8 Summary -- References -- Chapter 2 The Applications of Responsive Fluorescent Sensors to Biological Systems -- 2.1 Criteria for Biologically Relevant Fluorescent Sensors -- 2.2 Microscopy for Visualising Fluorescent Sensors -- 2.2.1 Important Considerations in Microscopy -- 2.2.2 Common Microscopy Techniques -- 2.3 Other Instrumental Techniques for Studying Cells Treated with Fluorescent Sensors -- 2.3.1 Flow Cytometry -- 2.3.2 Fluorescence Plate-readers -- 2.4 Biological Samples to Which Fluorescent Sensors Can Be Applied -- 2.4.1 Cultured Mammalian Cells -- 2.4.2 Bacteria -- 2.4.3 Plants -- 2.4.4 Multi-cellular Organisms -- 2.4.5 Towards In Vivo Imaging -- 2.5 Common Challenges and Misconceptions in the Applications of Fluorescent Sensors.
2.5.1 Important Considerations in Applying Sensors -- 2.5.2 Common Misconceptions About the Use of Sensors - The Bridge Between Multiple Disciplines -- 2.6 Conclusions -- References -- Chapter 3 Methods to Control the Subcellular Localisation of Fluorescent Sensors -- 3.1 Introduction -- 3.2 Targeting the Nucleus -- 3.3 Targeting Mitochondria -- 3.4 Targeting Lysosomes -- 3.5 Targeting Endosomes -- 3.6 Targeting Autophagic Compartments -- 3.7 Targeting Peroxisomes -- 3.8 Targeting the Endoplasmic Reticulum -- 3.9 Targeting the Golgi Apparatus -- 3.10 Targeting Lipid Droplets -- 3.11 Targeting the Plasma Membrane -- 3.12 Targeting the Cytoskeleton -- 3.13 Targeting the Cytosol -- 3.14 Trapping and Accumulation of Sensors -- References -- Chapter 4 Recognition-based Sensors for Cellular Imaging -- 4.1 Considerations for Recognition-based Sensing -- 4.1.1 Receptor-Analyte Recognition and Binding Affinity -- 4.1.2 Key Considerations to Enhance Selective Receptor to Analyte Recognition -- 4.2 Recognition-based Cation Sensing -- 4.2.1 Group I and II Metal Sensing -- 4.2.2 Essential Transition Metal Sensing -- 4.2.3 Toxic Metal Sensing -- 4.3 Recognition-based Anion Sensing -- 4.3.1 Anion Sensing Approaches -- 4.3.2 Halogen Ions Sensing -- 4.3.3 Inorganic Phosphates and Pyrophosphates -- 4.3.4 Bicarbonate, Hydrogen Sulfate, and Nitrate -- 4.4 Conclusions -- References -- Chapter 5 Activity-based Fluorescent Sensors and Their Applications in Biological Studies -- 5.1 Introduction -- 5.1.1 Design Principles -- 5.2 Oxidation Reactions for Sensing Oxidative Species -- 5.2.1 Fluorescent Sensors for Hydrogen Peroxide -- 5.2.2 Fluorescent Sensors for Peroxynitrite -- 5.2.3 Fluorescent Sensors for Hypochlorous Acid -- 5.2.4 Fluorescent Sensors for Nitric Oxide -- 5.2.5 Fluorescent Sensors for Singlet Oxygen -- 5.3 Reduction Reactions for Sensing Reductive Species. 5.3.1 Fluorescent Sensors for Hydrogen Sulfides -- 5.3.2 Fluorescent Sensors for Glutathione, Cysteine, and Homocysteine -- 5.3.3 Fluorescent Sensors for Selenocysteine -- 5.4 Reactions for Sensing Carbonyl Species -- 5.4.1 Fluorescent Sensors for Formaldehyde -- 5.4.2 Fluorescent Sensors for Methylglyoxal -- 5.5 Metal-mediated Reactions -- 5.6 Metal-sensing Reactions -- 5.7 Enzymatic Reactions -- 5.8 Reversible Reactions -- 5.8.1 Nucleophilic Conjugate Additions -- 5.8.2 Nucleophilic Addition -- 5.8.3 Imine Formation -- 5.8.4 Oxidation-Reduction Reactions -- 5.9 Analyte Regeneration -- 5.10 Summary -- References -- Chapter 6 Fluorescent Sensors of the Cellular Environment -- 6.1 Fluorescent Sensors for Polarity and Viscosity -- 6.1.1 The Biological Significance of Polarity and Viscosity -- 6.1.2 Twisted Intramolecular Charge Transfer as a Mechanism for Polarity and Viscosity Sensing -- 6.1.3 Polarity Sensors Based on Other Mechanisms -- 6.2 Fluorescent Sensors for pH -- 6.2.1 The Regulation of pH in Health and Disease -- 6.2.2 Considerations and Design Strategies for the Preparation of pH Sensors -- 6.2.3 Examples of pH Sensors -- 6.3 Fluorescent Redox Sensors for Biological Studies -- 6.3.1 The Regulation of Redox State in Health and Disease -- 6.3.2 Design Strategies of Fluorescent Redox Sensors and Key Examples -- 6.4 Conclusions -- References -- Chapter 7 Labelling Proteins and Biomolecules with Small Fluorescent Sensors -- 7.1 Labelling Biomolecules in Cells with Fluorescent Sensors -- 7.2 Small-molecule Modifications and Bioorthogonal Reactions -- 7.2.1 Polar Ketone and Aldehyde Condensations -- 7.2.2 Azide Bioorthogonal Chemistry -- 7.2.3 Tetrazine Ligation -- 7.2.4 Commercial Fluorescent Labels -- 7.3 Short peptide Recognition Sequences -- 7.4 Fusion Protein Tagging Systems -- 7.4.1 FKBP Tag -- 7.4.2 eDHFR Tag -- 7.4.3 PYP Tag. 7.4.4 SNAP-Tag and CLIP-Tag -- 7.4.5 HaloTag -- 7.5 Enzymatic Modifications for Labelling Proteins -- 7.5.1 The LAP-tag System -- 7.5.2 Protein Trans-splicing -- 7.6 Future Developments -- References -- Chapter 8 Future Directions of Fluorescence Sensors for Cellular Studies -- 8.1 Fluorescence Lifetime Imaging Microscopy -- 8.1.1 Introduction -- 8.1.2 Advantages of Fluorescence Lifetime Imaging Microscopy -- 8.1.3 Examples of Sensors for FLIM -- 8.1.4 Future Directions -- 8.2 Near-infrared Sensors -- 8.2.1 Strategies to Make NIR Sensors -- 8.2.2 NIR Fluorophore Scaffolds -- 8.2.3 Future Directions -- 8.3 Dual-analyte Sensing -- 8.3.1 Introduction -- 8.3.2 Recognition-Based Dual-analyte Sensors -- 8.3.3 Activity-based Dual-analyte Sensors -- 8.3.4 Mixed Dual-analyte Sensors -- 8.3.5 Sequence-specific Reactions -- 8.3.6 Conclusions and Future Directions -- 8.4 Super-resolution Microscopy -- 8.4.1 Introduction -- 8.4.2 Super-resolution Microscopy Techniques -- 8.4.3 Considerations for Use of Super Resolution Microscopy -- 8.4.4 Fluorescent Sensors for Super-resolution Microscopy -- 8.4.5 Future Directions -- 8.5 Multimodality -- 8.5.1 Introduction -- 8.5.2 Radioisotope Techniques -- 8.5.3 Computed Tomography -- 8.5.4 Magnetic Resonance Imaging -- 8.5.5 Photoacoustic Imaging -- 8.5.6 Vibrational Spectroscopy -- 8.5.7 Synchrotron X-ray Techniques -- 8.5.8 Mass Spectrometry -- 8.5.9 Electron Microscopy -- 8.5.10 Three or More Modalities -- 8.5.11 Future Directions -- References -- Index -- EULA. |
| Record Nr. | UNINA-9910590101003321 |
| Chichester, West Sussex : , : John Wiley & Sons, Incorporated, , 2022 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Synthese von fluoreszierenden calcium-sensoren und biomolekülen zur untersuchung physiologischer prozesse / / Julia Graf
| Synthese von fluoreszierenden calcium-sensoren und biomolekülen zur untersuchung physiologischer prozesse / / Julia Graf |
| Autore | Graf Julia |
| Pubbl/distr/stampa | Göttingen, [Germany] : , : Cuvillier Verlag, , 2017 |
| Descrizione fisica | 1 online resource (207 pages) : illustrations (some color) |
| Disciplina | 570.28 |
| Soggetto topico | Fluorescent probes |
| ISBN | 3-7369-8567-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | ger |
| Record Nr. | UNINA-9910794943303321 |
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Graf Julia
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| Göttingen, [Germany] : , : Cuvillier Verlag, , 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Synthese von fluoreszierenden calcium-sensoren und biomolekülen zur untersuchung physiologischer prozesse / / Julia Graf
| Synthese von fluoreszierenden calcium-sensoren und biomolekülen zur untersuchung physiologischer prozesse / / Julia Graf |
| Autore | Graf Julia |
| Pubbl/distr/stampa | Göttingen, [Germany] : , : Cuvillier Verlag, , 2017 |
| Descrizione fisica | 1 online resource (207 pages) : illustrations (some color) |
| Disciplina | 570.28 |
| Soggetto topico | Fluorescent probes |
| ISBN | 3-7369-8567-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | ger |
| Record Nr. | UNINA-9910822449803321 |
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Graf Julia
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| Göttingen, [Germany] : , : Cuvillier Verlag, , 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Soggetto topico
-
Fluorescent probes
(10)
- Fluorescence (3)
- Biosensors (2)
- Biology - Technique (1)
- Cell membranes (1)