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Application of diffractive lens arrays in confocal microscopy / / Zheng Li
| Application of diffractive lens arrays in confocal microscopy / / Zheng Li |
| Autore | Li Zheng |
| Pubbl/distr/stampa | Karlsruhe : , : KIT Scientific Publishing, , [2022] |
| Descrizione fisica | 1 online resource (xv, 148 pages) : illustrations |
| Disciplina | 502.82 |
| Collana | Schriftenreihe automatische Sichtprüfung und Bildverarbeitung |
| Soggetto topico |
Confocal microscopy
Fluorescence microscopy |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Abstract -- Kurzfassung -- Acknowledgements -- Notation -- Acronyms -- 1 Introduction -- 1.1 Motivation -- 1.2 Related work -- 1.3 Main contributions -- 1.4 Thesis outline -- 2 Theoretical Background -- 2.1 Diffraction simulation -- 2.1.1 Maxwell's equations -- 2.1.2 Scalar approximation and solutions -- 2.1.3 Numerical implementation -- 2.2 Theory of confocal microscopy -- 2.2.1 Focusing by a thin lens -- 2.2.2 PSF of a thin lens -- 2.2.3 PSF of a confocal scanning microscope -- 3 Design and Simulation -- 3.1 See-through DLA design -- 3.1.1 System overview -- 3.1.2 Design process -- 3.1.3 Simulation and optimization -- 3.2 Direct-imaging DLA design -- 3.2.1 System overview -- 3.2.2 Design process -- 3.2.3 Simulation and optimization -- 4 Experiment Results -- 4.1 Spot characterization -- 4.1.1 Spot measurement of See-through DLAs -- 4.1.2 Spot measurement of Direct-imaging DLAs -- 4.2 Lateral measurement -- 4.2.1 Lateral measurement by See-through DLAs -- 4.2.2 Lateral measurement by Direct-imaging DLAs -- 4.3 Axial measurement -- 4.3.1 Axial response of See-through DLAs -- 4.3.2 Axial response of Direct-imaging DLAs -- 4.3.3 Interference measurement by See-through DLAs -- 4.3.4 Measurement of a step height target -- 4.4 Fluorescence measurement -- 5 Conclusions -- 5.1 Summary of the work -- 5.2 Outlook -- Bibliography -- Publications -- Appendix -- A Kirchhoff's Diffraction Formulation. |
| Record Nr. | UNINA-9910580218603321 |
Li Zheng
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| Karlsruhe : , : KIT Scientific Publishing, , [2022] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Confocal and two-photon microscopy : foundations, applications, and advances / edited by Alberto Diaspro
| Confocal and two-photon microscopy : foundations, applications, and advances / edited by Alberto Diaspro |
| Pubbl/distr/stampa | New York : Wiley-Liss, c2002 |
| Descrizione fisica | xix, 567 p. : ill. ; 26 cm |
| Disciplina | 502.82 |
| Altri autori (Persone) | Diaspro, Alberto |
| Soggetto topico |
Confocal microscopy - methods
Fluorescence microscopy Three-dimensional imaging - Methodology |
| ISBN | 0471409200 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991001782369707536 |
| New York : Wiley-Liss, c2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
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Fluorescence microscopy : from principles to biological applications / / edited by Ulrich Kubitscheck
| Fluorescence microscopy : from principles to biological applications / / edited by Ulrich Kubitscheck |
| Autore | Kubitscheck Ulrich |
| Edizione | [2nd edition.] |
| Pubbl/distr/stampa | [Weinheim, Germany] : , : Wiley-VCH, , 2017 |
| Descrizione fisica | 1 online resource (507 pages) |
| Disciplina | 502.82 |
| Soggetto topico | Fluorescence microscopy |
| ISBN |
3-527-68774-2
1-5231-1530-0 3-527-68772-6 3-527-68773-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910270892803321 |
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Kubitscheck Ulrich
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| [Weinheim, Germany] : , : Wiley-VCH, , 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescence microscopy : from principles to biological applications / / edited by Ulrich Kubitscheck
| Fluorescence microscopy : from principles to biological applications / / edited by Ulrich Kubitscheck |
| Autore | Kubitscheck Ulrich |
| Edizione | [2nd edition.] |
| Pubbl/distr/stampa | [Weinheim, Germany] : , : Wiley-VCH, , 2017 |
| Descrizione fisica | 1 online resource (507 pages) |
| Disciplina | 502.82 |
| Soggetto topico | Fluorescence microscopy |
| ISBN |
3-527-68774-2
1-5231-1530-0 3-527-68772-6 3-527-68773-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910814764303321 |
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Kubitscheck Ulrich
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| [Weinheim, Germany] : , : Wiley-VCH, , 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescence microscopy [[electronic resource] ] : from principles to biological applications / / edited by Ulrich Kubitscheck
| Fluorescence microscopy [[electronic resource] ] : from principles to biological applications / / edited by Ulrich Kubitscheck |
| Pubbl/distr/stampa | Weinheim [Germany], : Wiley-Blackwell, 2013 |
| Descrizione fisica | 1 online resource (432 p.) |
| Disciplina | 502.82 |
| Altri autori (Persone) | KubitscheckUlrich |
| Soggetto topico |
Fluorescence microscopy
Microscopy |
| ISBN |
3-527-67159-5
3-527-67161-7 1-299-46465-3 3-527-67160-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Fluorescence Microscopy; Contents; Preface; List of Contributors; 1 Introduction to Optics and Photophysics; 1.1 Interference: Light as a Wave; 1.2 Two Effects of Interference: Diffraction and Refraction; 1.3 Optical Elements; 1.3.1 Lenses; 1.3.2 Metallic Mirror; 1.3.3 Dielectric Mirror; 1.3.4 Pinholes; 1.3.5 Filters; 1.3.6 Chromatic Reflectors; 1.4 The Far-Field, Near-Field, and Evanescent Waves; 1.5 Optical Aberrations; 1.6 Physical Background of Fluorescence; 1.7 Photons, Poisson Statistics, and AntiBunching; References; 2 Principles of Light Microscopy; 2.1 Introduction
2.2 Construction of Light Microscopes2.2.1 Components of Light Microscopes; 2.2.2 Imaging Path; 2.2.3 Magnification; 2.2.4 Angular and Numerical Aperture; 2.2.5 Field of View; 2.2.6 Illumination Beam Path; 2.3 Wave Optics and Resolution; 2.3.1 Wave Optical Description of the Imaging Process; 2.3.2 The Airy Function; 2.3.3 Point Spread Function and Optical Transfer Function; 2.3.4 Lateral and Axial Resolution; 2.3.5 Magnification and Resolution; 2.3.6 Depth of Field and Depth of Focus; 2.3.7 Over- and Under Sampling; 2.4 Apertures, Pupils, and Telecentricity; 2.5 Microscope Objectives 2.5.1 Objective Lens Design2.5.2 Light Collection Efficiency and Image Brightness; 2.5.3 Objective Lens Classes; 2.5.4 Immersion Media; 2.5.5 Special Applications; 2.6 Contrast; 2.6.1 Dark Field; 2.6.2 Phase Contrast; 2.6.3 Interference Contrast; 2.6.4 Advanced Topic: Differential Interference Contrast; 2.7 Summary; Acknowledgments; References; 3 Fluorescence Microscopy; 3.1 Features of Fluorescence Microscopy; 3.1.1 Image Contrast; 3.1.2 Specificity of Fluorescence Labeling; 3.1.3 Sensitivity of Detection; 3.2 A Fluorescence Microscope; 3.2.1 Principle of Operation 3.2.2 Sources of Exciting Light3.2.3 Optical Filters in a Fluorescence Microscope; 3.2.4 Electronic Filters; 3.2.5 Photodetectors for Fluorescence Microscopy; 3.2.6 CCD-Charge-Coupled Device; 3.2.7 Intensified CCD (ICCD); 3.2.8 Electron-Multiplying Charge-Coupled Device (EMCCD); 3.2.9 CMOS; 3.2.10 Scientific CMOS (sCMOS); 3.2.11 Features of CCD and CMOS Cameras; 3.2.12 Choosing a Digital Camera for Fluorescence Microscopy; 3.2.13 Photomultiplier Tube (PMT); 3.2.14 Avalanche Photodiode (APD); 3.3 Types of Noise in a Digital Microscopy Image; 3.4 Quantitative Fluorescence Microscopy 3.4.1 Measurements of Fluorescence Intensity and Concentration of the Labeled Target3.4.2 Ratiometric Measurements (Ca++, pH); 3.4.3 Measurements of Dimensions in 3D Fluorescence Microscopy; 3.4.4 Measurements of Exciting Light Intensity; 3.4.5 Technical Tips for Quantitative Fluorescence Microscopy; 3.5 Limitations of Fluorescence Microscopy; 3.5.1 Photobleaching; 3.5.2 Reversible Photobleaching under Oxidizing or Reducing Conditions; 3.5.3 Phototoxicity; 3.5.4 Optical Resolution; 3.5.5 Misrepresentation of Small Objects; 3.6 Current Avenues of Development; References; Further Reading Recommended Internet Resources |
| Record Nr. | UNINA-9910139007403321 |
| Weinheim [Germany], : Wiley-Blackwell, 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescence microscopy in life sciences / / Juan Carlos Stockert & Alfonso Blázquez-Castro
| Fluorescence microscopy in life sciences / / Juan Carlos Stockert & Alfonso Blázquez-Castro |
| Autore | Stockert Juan Carlos |
| Pubbl/distr/stampa | Sharjah, United Arab Emirates : , : Bentham eBooks, , 2017 |
| Descrizione fisica | 1 online resource (731 pages) : illustrations (some color) |
| Disciplina | 570.282 |
| Soggetto topico | Fluorescence microscopy |
| ISBN | 1-68108-518-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910796630403321 |
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Stockert Juan Carlos
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| Sharjah, United Arab Emirates : , : Bentham eBooks, , 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescence microscopy in life sciences / / Juan Carlos Stockert & Alfonso Blázquez-Castro
| Fluorescence microscopy in life sciences / / Juan Carlos Stockert & Alfonso Blázquez-Castro |
| Autore | Stockert Juan Carlos |
| Pubbl/distr/stampa | Sharjah, United Arab Emirates : , : Bentham eBooks, , 2017 |
| Descrizione fisica | 1 online resource (731 pages) : illustrations (some color) |
| Disciplina | 570.282 |
| Soggetto topico | Fluorescence microscopy |
| ISBN | 1-68108-518-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910816961803321 |
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Stockert Juan Carlos
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| Sharjah, United Arab Emirates : , : Bentham eBooks, , 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescence molecular tomography : principles and applications / / Huabei Jiang
| Fluorescence molecular tomography : principles and applications / / Huabei Jiang |
| Autore | Jiang Huabei |
| Pubbl/distr/stampa | Cham, Switzerland : , : Springer Nature Switzerland AG, , [2023] |
| Descrizione fisica | 1 online resource (135 pages) |
| Disciplina | 570.282 |
| Soggetto topico |
Fluorescence microscopy
Tomography Biomedical engineering |
| ISBN |
9783031100048
9783031100031 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Intro -- Preface -- Contents -- Chapter 1: Introduction -- 1.1 Fluorescence Molecular Tomography -- 1.2 Mathematic Image Reconstruction -- 1.2.1 Forward Problem: Fluorescent Photon Diffusion/Transport Model -- 1.2.2 Inverse Problem -- 1.3 Fluorescent Probes -- Chapter 2: Reconstruction Algorithms -- 2.1 Linear Reconstruction Algorithm -- 2.2 Nonlinear Reconstruction Algorithm -- 2.2.1 Algorithm Implementation -- 2.2.2 Results -- 2.2.3 Discussion -- Chapter 3: Instrumentation -- 3.1 Time-Domain FMT System -- 3.2 Frequency-Domain FMT System -- 3.3 CW-Domain FMT System -- 3.4 Contact vs. Noncontact Light Detection Scheme -- Chapter 4: Image Enhancing Schemes -- 4.1 Dual Mesh Scheme -- 4.2 RTE-Based Fluorescence Molecular Tomography -- 4.3 The Third-Order Diffusion Equations-Based FMT -- 4.4 DOT-Guided FMT -- 4.4.1 Methods and Materials -- 4.4.2 Results and Discussion -- 4.5 Fluorophore Lifetime Imaging -- 4.5.1 Methods and Materials -- 4.5.2 Results and Discussion -- 4.6 Full Density FMT -- 4.6.1 Methods -- 4.6.2 Results and Discussion -- Chapter 5: Bioluminescence Tomography -- 5.1 BLT Reconstruction Algorithm -- 5.1.1 Simulations -- 5.1.2 Experiments -- 5.1.3 Results and Discussion -- 5.2 DOT-Guided Bioluminescence Tomography -- 5.2.1 Methods -- 5.2.2 Results and Discussion -- Chapter 6: Multimodal Approaches -- 6.1 FMT/PAT -- 6.1.1 Methods -- 6.1.2 Results and Discussion -- 6.1.2.1 Comparison of Image Pattern -- 6.1.2.2 Comparison of Spatial Resolution -- 6.1.2.3 Comparison of Sensitivity -- 6.2 FMT/CT -- 6.3 FMT/Phase-Contrast CT -- 6.4 FMT/MRI -- 6.5 FMT/CT/SPECT -- Chapter 7: Miniaturized Fluorescence Molecular Tomography -- 7.1 Handheld FMT -- 7.1.1 Methods -- 7.1.2 Results and Discussion -- 7.1.2.1 Simulations -- 7.1.2.2 Phantom Experiments -- 7.1.2.3 Mouse Experiments -- 7.2 FMT Pen -- 7.2.1 Methods -- 7.2.2 Results and Discussion.
7.2.2.1 Phantom Experiments -- 7.2.2.2 Animal Experiments -- 7.3 Endoscopic FMT -- 7.3.1 Methods -- 7.3.2 Results and Discussion -- Chapter 8: Animal Studies and Clinical Applications -- 8.1 Fruit Flies -- 8.1.1 Pupa with Dye-Containing Microtube -- 8.1.2 In Vivo Pupa with DsRed Reporter -- 8.2 Tumor Imaging in Animals -- 8.2.1 Ovarian Tumor -- 8.2.1.1 Methods -- Cell Lines and HER-2/Neu-Specific Affibody Conjugation to Magnetic Iron Oxide Nanoparticles (IONPs) -- Orthotopic Human Ovarian Cancer Xenograft Model -- Fluorescence Molecular Tomography Imaging System -- Photoacoustic Tomography System -- In Vivo Imaging in Animal Tumor Models -- Histology Analysis -- 8.2.1.2 Results and Discussion -- 8.2.2 Lung Tumor -- 8.2.2.1 Methods -- 8.2.2.2 Results and Discussion -- 8.2.3 Brain Tumor -- 8.2.3.1 Methods -- 8.2.3.2 Results and Discussion -- 8.3 Breast Tumor Imaging in Humans -- 8.3.1 Methods -- 8.3.2 Results and Discussion -- 8.4 Tumor Response to Therapies -- 8.4.1 Methods -- 8.4.2 Results and Discussion -- 8.5 Quantification of Receptor Density -- 8.5.1 Methods -- 8.5.2 Results and Discussion -- 8.6 Bone Remodeling -- 8.6.1 Methods -- 8.6.2 Results and Discussion -- 8.7 Alzheimer´s Disease -- 8.7.1 Methods -- 8.7.2 Results and Discussion -- 8.8 Cardiovascular Disease -- 8.8.1 Methods -- 8.8.2 Results and Discussion -- Bibliography -- Index. |
| Record Nr. | UNINA-9910627270103321 |
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Jiang Huabei
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| Cham, Switzerland : , : Springer Nature Switzerland AG, , [2023] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Fluorescence spectroscopy and microscopy in biology / / Radek Sachl and Mariana Amaro, editors
| Fluorescence spectroscopy and microscopy in biology / / Radek Sachl and Mariana Amaro, editors |
| Edizione | [1st ed. 2023.] |
| Pubbl/distr/stampa | Cham, Switzerland : , : Springer Nature Switzerland AG, , [2023] |
| Descrizione fisica | 1 online resource (529 pages) |
| Disciplina | 502.82 |
| Collana | Springer Series on Fluorescence, Methods and Applications |
| Soggetto topico |
Fluorescence microscopy
Fluorescence spectroscopy |
| ISBN |
9783031303623
9783031303616 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Part I - Fluorescence Spectroscopy: Basics and Advanced Approaches -- Chapter 1 Choosing the right fluorescent probe -- Chapter 2 Fluorescence kinetics and time-resolved measurement -- Chapter 3 Quantitative Approach to Applications of Electronic Energy Transfer (EET) -- Chapter 4 Single-molecule FRET: principles and analysis -- Chapter 5 Principles of fluorescence correlation and dual-color cross-correlation spectroscopy -- Part II - Fluorescence Microscopy: Basics and Advanced Approaches -- Chapter 6 Introduction to Fluorescence Microscopy -- Chapter 7 Wohland STED and RESOLFT fluorescent nanoscopy -- Chapter 8 Fluorescence Correlation Spectroscopy in Space and Time -- Part C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes -- Chapter 9 Determination of Biomolecular Oligomerization in the Live Cell Plasma Membrane via Single-molecule Brightness and Co-localization Analysis -- Chapter 10 Quantitative photoactivated localization microscopy of membrane receptor oligomers -- Chapter 11 Diffusion measurements at the nanoscale with STED-FCS -- Chapter 12 Single molecule microscopy methods to study mitochondrial processes -- Chapter 13 Transient state (TRAST) spectroscopy and imaging – exploiting the rich information source of fluorophore dark state transitions in biomolecular and cellular studies -- Chapter 14 The analysis of in-membrane nanoscopic aggregation of lipids and proteins by MC-FRET -- Part D - Applications of Fluorescence Spectroscopy to protein studies -- Chapter 15 Single-molecule fluorescence spectroscopy of intrinsically disordered proteins -- Chapter 16 Insights into the conformational dynamics of potassium channels using homo-FRET approaches -- Chapter 17 Intrinsic fluorescence kinetics in proteins -- Chapter 18 Dynamics and hydration of proteins viewed by fluorescence methods: investigations famor protein engineering and synthetic biology. |
| Record Nr. | UNINA-9910720085403321 |
| Cham, Switzerland : , : Springer Nature Switzerland AG, , [2023] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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The fluorescent protein revolution / / editors, Richard N. Day, Michael W. Davidson
| The fluorescent protein revolution / / editors, Richard N. Day, Michael W. Davidson |
| Pubbl/distr/stampa | Boca Raton : , : CRC Press/Taylor & Francis Group, , [2014] |
| Descrizione fisica | 1 online resource (340 p.) |
| Disciplina | 616.07/58 |
| Collana | Series in cellular and clinical imaging |
| Soggetto topico |
Cytodiagnosis
Fluorescence microscopy |
| ISBN |
0-429-19353-X
1-4398-7508-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Contents; Series Preface; Preface; Editors; Contributors; Part I: History and perspective; Chapter 1: Introduction to fluorescent proteins; Part II: Photophysical properties of fluorescent proteins; Chapter 2 Lessons learned from structural studies of fluorescent proteins; Chapter 3: Optimization of fluorescent proteins; Chapter 4: Development of new colors from coral fluorescent proteins; Chapter 5: Red fluorescent proteins: multipurpose markers for live-cell imaging; Chapter 6: Optical highlighter photophysical properties
Chapter 7: Far-red and near-infrared fluorescent proteinsPart III: Applications; Chapter 8: Genetically encoded fluorescent proteins and FRAP; Chapter 9: Optical highlighters: Applications to cell biology; Chapter 10: Optogenetic tools derived from plant photoreceptors; Chapter 11: Fluorescent proteins for FRET : monitoring protein interactions in living cells; Chapter 12: Super resolution techniques using fluorescent protein technology; Chapter 13: In vivo imaging revolution made by fluorescent proteins; Back Cover |
| Record Nr. | UNINA-9910787846203321 |
| Boca Raton : , : CRC Press/Taylor & Francis Group, , [2014] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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