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Enzimologia / Michele Perella ; con contributi di Oreste Brenna, Mario Pace
| Enzimologia / Michele Perella ; con contributi di Oreste Brenna, Mario Pace |
| Autore | Perrella, Michele |
| Pubbl/distr/stampa | Milano : Clesav, 1981 |
| Descrizione fisica | 238 p. ; 24 cm |
| Disciplina | 572.7 |
| Altri autori (Persone) |
Pace, Mario
Brenna, Oreste |
| Soggetto topico | Enzymes - Analysis |
| ISBN | 8870640264 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | ita |
| Record Nr. | UNISALENTO-991003466449707536 |
Perrella, Michele
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| Milano : Clesav, 1981 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
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Enzyme assays [[electronic resource] ] : high-throughput screening, genetic selection, and fingerprinting / / edited by Jean-Louis Reymond
| Enzyme assays [[electronic resource] ] : high-throughput screening, genetic selection, and fingerprinting / / edited by Jean-Louis Reymond |
| Pubbl/distr/stampa | Weinheim, Germany, : Wiley-VCH, c2006 |
| Descrizione fisica | 1 online resource (388 p.) |
| Disciplina | 572.7 |
| Altri autori (Persone) | ReymondJean-Louis |
| Soggetto topico | Enzymes - Analysis |
| ISBN |
1-280-85428-6
9786610854288 3-527-60784-6 3-527-60721-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Enzyme Assays; Contents; Preface; List of Contributors; Introduction; Enzyme Assays; Part I: High-throughput Screening; Part II: Genetic Selection; Part III: Enzyme Fingerprinting; Enzyme Assays in Other Areas; How to Use this Book; Part I High-throughput Screening; 1 Quantitative Assay of Hydrolases for Activity and Selectivity Using Color Changes; 1.1 Overview; 1.2 Direct Assays Using Chromogenic Substrates; 1.3 Indirect Assays Using Coupled Reactions - pH Indicators; 1.3.1 Overview of Quantitative Use of pH Indicator Assay; 1.3.2 Applications
1.3.2.1 Searching for an Active Hydrolase (Testing Many Hydrolases Toward One Substrate)1.3.2.2 Substrate Mapping of New Hydrolases (Testing Many Substrates Toward Hydrolase); 1.3.3 Comparison with Other Methods; 1.4 Estimating and Measuring Selectivity; 1.4.1 Estimating Selectivity without a Reference Compound; 1.4.2 Quantitative Measure of Selectivity Using a Reference Compound (Quick E and Related Methods); 1.4.2.1 Chromogenic Substrate; 1.4.2.2 pH Indicators; 1.4.3 Application; 1.4.3.1 Substrate Mapping of Hydrolases; 1.4.3.2 Screening of Mutants in Directed Evolution 1.4.4 Advantages and DisadvantagesReferences; 2 High-throughput Screening Systems for Assaying the Enantioselectivity of Enzymes; 2.1 Introduction; 2.2 UV/Vis Spectroscopy-based Assays; 2.2.1 Assay for Screening Lipases or Esterases in the Kinetic Resolution of Chiral p-Nitrophenyl Esters; 2.2.2 Enzyme-coupled UV/Vis-based Assay for Lipases and Esterases; 2.2.3 Enzymatic Method for Determining Enantiomeric Excess (EMDee); 2.2.4 UV/Vis-based Enzyme Immunoassay as a Means to Measure Enantiomeric Excess; 2.2.5 Other UV/Vis-based ee-Assays; 2.3 Assays Using Fluorescence 2.3.1 Umbelliferone-based Systems2.3.2 Fluorescence-based Assay Using DNA Microarrays; 2.3.3 Other Fluorescence-based ee-Assays; 2.4 Assays Based on Mass Spectrometry (MS); 2.4.1 MS-based Assay Using Isotope Labeling; 2.5 Assays Based on Nuclear Magnetic Resonance Spectroscopy; 2.6 Assay Based on Fourier Transform Infrared Spectroscopy for Assaying Lipases or Esterases; 2.7 Assays Based on Gas Chromatography; 2.8 Assays Based on HPLC; 2.9 Assays Based on Capillary Array Electrophoresis; 2.10 Assays Based on Circular Dichroism (CD) 2.11 Assay Based on Surface-enhanced Resonance Raman Scattering2.12 Conclusions; References; 3 High-throughput Screening Methods Developed for Oxidoreductases; 3.1 Introduction; 3.2 High-throughput Methods for Various Oxidoreductases; 3.2.1 Dehydrogenases; 3.2.1.1 Colorimetric Screen Based on NAD(P)H Generation; 3.2.1.2 Screens Based on NAD(P)H Depletion; 3.2.2 Oxidases; 3.2.2.1 Galactose Oxidase; 3.2.2.2 D-Amino Acid Oxidase; 3.2.2.3 Peroxidases; 3.2.3 Oxygenases; 3.2.3.1 Assays Based on Optical Properties of Substrates and Products 3.2.3.2 Assays Based on Gibbs' Reagent and 4-Aminoantipyrine |
| Record Nr. | UNINA-9910143962403321 |
| Weinheim, Germany, : Wiley-VCH, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Enzyme assays [[electronic resource] ] : high-throughput screening, genetic selection, and fingerprinting / / edited by Jean-Louis Reymond
| Enzyme assays [[electronic resource] ] : high-throughput screening, genetic selection, and fingerprinting / / edited by Jean-Louis Reymond |
| Pubbl/distr/stampa | Weinheim, Germany, : Wiley-VCH, c2006 |
| Descrizione fisica | 1 online resource (388 p.) |
| Disciplina | 572.7 |
| Altri autori (Persone) | ReymondJean-Louis |
| Soggetto topico | Enzymes - Analysis |
| ISBN |
1-280-85428-6
9786610854288 3-527-60784-6 3-527-60721-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Enzyme Assays; Contents; Preface; List of Contributors; Introduction; Enzyme Assays; Part I: High-throughput Screening; Part II: Genetic Selection; Part III: Enzyme Fingerprinting; Enzyme Assays in Other Areas; How to Use this Book; Part I High-throughput Screening; 1 Quantitative Assay of Hydrolases for Activity and Selectivity Using Color Changes; 1.1 Overview; 1.2 Direct Assays Using Chromogenic Substrates; 1.3 Indirect Assays Using Coupled Reactions - pH Indicators; 1.3.1 Overview of Quantitative Use of pH Indicator Assay; 1.3.2 Applications
1.3.2.1 Searching for an Active Hydrolase (Testing Many Hydrolases Toward One Substrate)1.3.2.2 Substrate Mapping of New Hydrolases (Testing Many Substrates Toward Hydrolase); 1.3.3 Comparison with Other Methods; 1.4 Estimating and Measuring Selectivity; 1.4.1 Estimating Selectivity without a Reference Compound; 1.4.2 Quantitative Measure of Selectivity Using a Reference Compound (Quick E and Related Methods); 1.4.2.1 Chromogenic Substrate; 1.4.2.2 pH Indicators; 1.4.3 Application; 1.4.3.1 Substrate Mapping of Hydrolases; 1.4.3.2 Screening of Mutants in Directed Evolution 1.4.4 Advantages and DisadvantagesReferences; 2 High-throughput Screening Systems for Assaying the Enantioselectivity of Enzymes; 2.1 Introduction; 2.2 UV/Vis Spectroscopy-based Assays; 2.2.1 Assay for Screening Lipases or Esterases in the Kinetic Resolution of Chiral p-Nitrophenyl Esters; 2.2.2 Enzyme-coupled UV/Vis-based Assay for Lipases and Esterases; 2.2.3 Enzymatic Method for Determining Enantiomeric Excess (EMDee); 2.2.4 UV/Vis-based Enzyme Immunoassay as a Means to Measure Enantiomeric Excess; 2.2.5 Other UV/Vis-based ee-Assays; 2.3 Assays Using Fluorescence 2.3.1 Umbelliferone-based Systems2.3.2 Fluorescence-based Assay Using DNA Microarrays; 2.3.3 Other Fluorescence-based ee-Assays; 2.4 Assays Based on Mass Spectrometry (MS); 2.4.1 MS-based Assay Using Isotope Labeling; 2.5 Assays Based on Nuclear Magnetic Resonance Spectroscopy; 2.6 Assay Based on Fourier Transform Infrared Spectroscopy for Assaying Lipases or Esterases; 2.7 Assays Based on Gas Chromatography; 2.8 Assays Based on HPLC; 2.9 Assays Based on Capillary Array Electrophoresis; 2.10 Assays Based on Circular Dichroism (CD) 2.11 Assay Based on Surface-enhanced Resonance Raman Scattering2.12 Conclusions; References; 3 High-throughput Screening Methods Developed for Oxidoreductases; 3.1 Introduction; 3.2 High-throughput Methods for Various Oxidoreductases; 3.2.1 Dehydrogenases; 3.2.1.1 Colorimetric Screen Based on NAD(P)H Generation; 3.2.1.2 Screens Based on NAD(P)H Depletion; 3.2.2 Oxidases; 3.2.2.1 Galactose Oxidase; 3.2.2.2 D-Amino Acid Oxidase; 3.2.2.3 Peroxidases; 3.2.3 Oxygenases; 3.2.3.1 Assays Based on Optical Properties of Substrates and Products 3.2.3.2 Assays Based on Gibbs' Reagent and 4-Aminoantipyrine |
| Record Nr. | UNINA-9910830562203321 |
| Weinheim, Germany, : Wiley-VCH, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Enzyme assays : high-throughput screening, genetic selection, and fingerprinting / / edited by Jean-Louis Reymond
| Enzyme assays : high-throughput screening, genetic selection, and fingerprinting / / edited by Jean-Louis Reymond |
| Pubbl/distr/stampa | Weinheim, Germany, : Wiley-VCH, c2006 |
| Descrizione fisica | 1 online resource (388 p.) |
| Disciplina | 572.76 |
| Altri autori (Persone) | ReymondJean-Louis |
| Soggetto topico | Enzymes - Analysis |
| ISBN |
1-280-85428-6
9786610854288 3-527-60784-6 3-527-60721-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Enzyme Assays; Contents; Preface; List of Contributors; Introduction; Enzyme Assays; Part I: High-throughput Screening; Part II: Genetic Selection; Part III: Enzyme Fingerprinting; Enzyme Assays in Other Areas; How to Use this Book; Part I High-throughput Screening; 1 Quantitative Assay of Hydrolases for Activity and Selectivity Using Color Changes; 1.1 Overview; 1.2 Direct Assays Using Chromogenic Substrates; 1.3 Indirect Assays Using Coupled Reactions - pH Indicators; 1.3.1 Overview of Quantitative Use of pH Indicator Assay; 1.3.2 Applications
1.3.2.1 Searching for an Active Hydrolase (Testing Many Hydrolases Toward One Substrate)1.3.2.2 Substrate Mapping of New Hydrolases (Testing Many Substrates Toward Hydrolase); 1.3.3 Comparison with Other Methods; 1.4 Estimating and Measuring Selectivity; 1.4.1 Estimating Selectivity without a Reference Compound; 1.4.2 Quantitative Measure of Selectivity Using a Reference Compound (Quick E and Related Methods); 1.4.2.1 Chromogenic Substrate; 1.4.2.2 pH Indicators; 1.4.3 Application; 1.4.3.1 Substrate Mapping of Hydrolases; 1.4.3.2 Screening of Mutants in Directed Evolution 1.4.4 Advantages and DisadvantagesReferences; 2 High-throughput Screening Systems for Assaying the Enantioselectivity of Enzymes; 2.1 Introduction; 2.2 UV/Vis Spectroscopy-based Assays; 2.2.1 Assay for Screening Lipases or Esterases in the Kinetic Resolution of Chiral p-Nitrophenyl Esters; 2.2.2 Enzyme-coupled UV/Vis-based Assay for Lipases and Esterases; 2.2.3 Enzymatic Method for Determining Enantiomeric Excess (EMDee); 2.2.4 UV/Vis-based Enzyme Immunoassay as a Means to Measure Enantiomeric Excess; 2.2.5 Other UV/Vis-based ee-Assays; 2.3 Assays Using Fluorescence 2.3.1 Umbelliferone-based Systems2.3.2 Fluorescence-based Assay Using DNA Microarrays; 2.3.3 Other Fluorescence-based ee-Assays; 2.4 Assays Based on Mass Spectrometry (MS); 2.4.1 MS-based Assay Using Isotope Labeling; 2.5 Assays Based on Nuclear Magnetic Resonance Spectroscopy; 2.6 Assay Based on Fourier Transform Infrared Spectroscopy for Assaying Lipases or Esterases; 2.7 Assays Based on Gas Chromatography; 2.8 Assays Based on HPLC; 2.9 Assays Based on Capillary Array Electrophoresis; 2.10 Assays Based on Circular Dichroism (CD) 2.11 Assay Based on Surface-enhanced Resonance Raman Scattering2.12 Conclusions; References; 3 High-throughput Screening Methods Developed for Oxidoreductases; 3.1 Introduction; 3.2 High-throughput Methods for Various Oxidoreductases; 3.2.1 Dehydrogenases; 3.2.1.1 Colorimetric Screen Based on NAD(P)H Generation; 3.2.1.2 Screens Based on NAD(P)H Depletion; 3.2.2 Oxidases; 3.2.2.1 Galactose Oxidase; 3.2.2.2 D-Amino Acid Oxidase; 3.2.2.3 Peroxidases; 3.2.3 Oxygenases; 3.2.3.1 Assays Based on Optical Properties of Substrates and Products 3.2.3.2 Assays Based on Gibbs' Reagent and 4-Aminoantipyrine |
| Record Nr. | UNINA-9910877202003321 |
| Weinheim, Germany, : Wiley-VCH, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Enzyme assays : a practical approach / edited by Robert Eisenthal and Michael J. Danson
| Enzyme assays : a practical approach / edited by Robert Eisenthal and Michael J. Danson |
| Pubbl/distr/stampa | Oxford [England] : IRL Press, c1992 |
| Descrizione fisica | xxiv, 351 p. : ill. ; 25 cm |
| Disciplina |
574.1925
572.7 |
| Altri autori (Persone) |
Danson, Michael J.
Eisenthal, Robert |
| Collana | The Practical approach series |
| Soggetto topico |
Enzymes - Analysis
Enzymes - Purification |
| ISBN | 0199631425 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991003466519707536 |
| Oxford [England] : IRL Press, c1992 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
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Preparation and assay of enzymes : preparation and assay of substrates : special techniques / edited by Sidney P. Colowick and Nathan O. Kaplan
| Preparation and assay of enzymes : preparation and assay of substrates : special techniques / edited by Sidney P. Colowick and Nathan O. Kaplan |
| Pubbl/distr/stampa | New York [etc.] : Academic press, 1963 |
| Descrizione fisica | xxiii, 1054 p. : ill. ; 24 cm |
| Disciplina | 572 |
| Altri autori (Persone) |
Kaplan, Nathan Oram
Colowick, Sidney P. |
| Collana | Methods in enzymology ; 006 |
| Soggetto topico |
Enzimi
Enzymes - Analysis |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991003266819707536 |
| New York [etc.] : Academic press, 1963 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
| ||
Principi di analisi enzimatica / Hans U. Bergmeyer, Karlfried Gaven
| Principi di analisi enzimatica / Hans U. Bergmeyer, Karlfried Gaven |
| Autore | Bergmeyer, Hans U. |
| Pubbl/distr/stampa | Padova : Piccin, c1982 |
| Descrizione fisica | 260 p. : ill. ; 25 cm |
| Disciplina | 572.7 |
| Altri autori (Persone) | Gaven, Karlfriedauthor |
| Soggetto topico | Enzymes - Analysis |
| ISBN | 8821209474 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | ita |
| Record Nr. | UNISALENTO-991003546739707536 |
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Bergmeyer, Hans U.
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| Padova : Piccin, c1982 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
| ||
Trends in enzyme histochemistry and cytochemistry
| Trends in enzyme histochemistry and cytochemistry |
| Pubbl/distr/stampa | Amsterdam ; ; New York : , : Excerpta Medica |
| Descrizione fisica | 1 online resource (324 pages) : illustrations |
| Disciplina | 574.19/25 |
| Collana | Ciba Foundation symposium |
| Soggetto topico |
Enzymes - Analysis
Histochemistry Cytochemistry |
| ISBN |
1-280-78385-0
9786613694249 0-470-72056-5 0-470-71824-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Trends in Enzyme Histochemistry and Cytochemistry; Contents; Preface; Introduction; Criteria for the validation of quantitative histochemical enzyme techniques; Discussion; Qualitative cytological criteria for the validation of enzyme histochemical techniques; Discussion; General Discussion I: Validation; Conventional techniques for membrane-bound enzymes; Discussion; Tissue stabilizer methods in histochemistry; Discussion; Interim summary I; Semipermeable membrane techniques in quantitative enzyme histochemistry; Discussion
Microphotometric determination of enzyme activities in cryostat sections by the gel film technique; Discussion; Assessment of immunocytochemical techniques with particular reference to the mixed-aggregation immunocytochemical technique; Discussion; Quantitative cytochemical analysis of (single) cultured cells; Discussion; Microdensitometry; Discussion; General Discussion II; Interim summary II; Microscopic cytochemistry as matrix chemistry; Discussion; The constant proportion enzyme group concept in the selection of reference enzymes in metabolism; Discussion Use of enzyme activities as indices of maximum rates of fuel utilization; The place of histochemical techniques in toxicology, pharmacology and pathology; Discussion; Appropriate technology for the quantitative assessment of the final reaction product of histochemical techniques; Discussion; Chairman's concluding remarks; Index of contributors; Subject index |
| Record Nr. | UNINA-9910144657303321 |
| Amsterdam ; ; New York : , : Excerpta Medica | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Trends in enzyme histochemistry and cytochemistry
| Trends in enzyme histochemistry and cytochemistry |
| Pubbl/distr/stampa | Amsterdam ; ; New York : , : Excerpta Medica |
| Descrizione fisica | 1 online resource (324 pages) : illustrations |
| Disciplina | 574.19/25 |
| Collana | Ciba Foundation symposium |
| Soggetto topico |
Enzymes - Analysis
Histochemistry Cytochemistry |
| ISBN |
1-280-78385-0
9786613694249 0-470-72056-5 0-470-71824-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Trends in Enzyme Histochemistry and Cytochemistry; Contents; Preface; Introduction; Criteria for the validation of quantitative histochemical enzyme techniques; Discussion; Qualitative cytological criteria for the validation of enzyme histochemical techniques; Discussion; General Discussion I: Validation; Conventional techniques for membrane-bound enzymes; Discussion; Tissue stabilizer methods in histochemistry; Discussion; Interim summary I; Semipermeable membrane techniques in quantitative enzyme histochemistry; Discussion
Microphotometric determination of enzyme activities in cryostat sections by the gel film technique; Discussion; Assessment of immunocytochemical techniques with particular reference to the mixed-aggregation immunocytochemical technique; Discussion; Quantitative cytochemical analysis of (single) cultured cells; Discussion; Microdensitometry; Discussion; General Discussion II; Interim summary II; Microscopic cytochemistry as matrix chemistry; Discussion; The constant proportion enzyme group concept in the selection of reference enzymes in metabolism; Discussion Use of enzyme activities as indices of maximum rates of fuel utilization; The place of histochemical techniques in toxicology, pharmacology and pathology; Discussion; Appropriate technology for the quantitative assessment of the final reaction product of histochemical techniques; Discussion; Chairman's concluding remarks; Index of contributors; Subject index |
| Record Nr. | UNISA-996206964603316 |
| Amsterdam ; ; New York : , : Excerpta Medica | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Trends in enzyme histochemistry and cytochemistry
| Trends in enzyme histochemistry and cytochemistry |
| Pubbl/distr/stampa | Amsterdam ; ; New York : , : Excerpta Medica |
| Descrizione fisica | 1 online resource (324 pages) : illustrations |
| Disciplina | 574.19/25 |
| Collana | Ciba Foundation symposium |
| Soggetto topico |
Enzymes - Analysis
Histochemistry Cytochemistry |
| ISBN |
1-280-78385-0
9786613694249 0-470-72056-5 0-470-71824-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Trends in Enzyme Histochemistry and Cytochemistry; Contents; Preface; Introduction; Criteria for the validation of quantitative histochemical enzyme techniques; Discussion; Qualitative cytological criteria for the validation of enzyme histochemical techniques; Discussion; General Discussion I: Validation; Conventional techniques for membrane-bound enzymes; Discussion; Tissue stabilizer methods in histochemistry; Discussion; Interim summary I; Semipermeable membrane techniques in quantitative enzyme histochemistry; Discussion
Microphotometric determination of enzyme activities in cryostat sections by the gel film technique; Discussion; Assessment of immunocytochemical techniques with particular reference to the mixed-aggregation immunocytochemical technique; Discussion; Quantitative cytochemical analysis of (single) cultured cells; Discussion; Microdensitometry; Discussion; General Discussion II; Interim summary II; Microscopic cytochemistry as matrix chemistry; Discussion; The constant proportion enzyme group concept in the selection of reference enzymes in metabolism; Discussion Use of enzyme activities as indices of maximum rates of fuel utilization; The place of histochemical techniques in toxicology, pharmacology and pathology; Discussion; Appropriate technology for the quantitative assessment of the final reaction product of histochemical techniques; Discussion; Chairman's concluding remarks; Index of contributors; Subject index |
| Record Nr. | UNINA-9910811998703321 |
| Amsterdam ; ; New York : , : Excerpta Medica | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Soggetto topico
-
Enzymes - Analysis
(12)
- Cytochemistry (3)
- Histochemistry (3)
- Biomass energy (1)
- Enzimi (1)