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Electrophoresis in practice : a guide to methods and applications of DNA and protein separations / / Reiner Westermeier
| Electrophoresis in practice : a guide to methods and applications of DNA and protein separations / / Reiner Westermeier |
| Autore | Westermeier Reiner |
| Edizione | [Fifth edition.] |
| Pubbl/distr/stampa | Weinheim, Germany : , : Wiley-VCH Verlag, , 2016 |
| Descrizione fisica | 1 online resource (532 pages) |
| Disciplina | 543/.4 |
| Soggetto topico |
Electrophoresis
DNA - Separation Proteins - Separation |
| ISBN |
3-527-69516-8
3-527-69518-4 |
| Classificazione |
433.4
547.7/04572 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Title Page; Copyright; Table of Contents; Foreword; Abbreviations, Symbols, Units; Preface; German Version; English Version, Fifth Edition; Part I: Fundamentals; Chapter 1: Electrophoresis; 1.1 General; 1.2 Electrophoresis in Nonrestrictive Gels; 1.3 Electrophoresis in Restrictive Gels; References; Chapter 2: Isotachophoresis; 2.1 Migration with the Same Speed; 2.2 "Ion Train" Separation; 2.3 Zone Sharpening Effect; 2.4 Concentration Regulation Effect; 2.5 Quantitative Analysis; References; Chapter 3: Isoelectric Focusing; 3.1 Principles; 3.2 Gels for IEF; 3.3 Temperature
3.4 Controlling the pH Gradient; 3.5 Kinds of pH Gradients; 3.6 Protein Detection in IEF Gels; 3.7 Preparative Isoelectric Focusing; 3.8 Titration Curve Analysis; References; Chapter 4: High-Resolution Two-Dimensional Electrophoresis; 4.1 IEF in Immobilized pH Gradient Strips; 4.2 SDS-PAGE; 4.3 Proteomics; References; Chapter 5: Protein Sample Preparation; 5.1 Protein Quantification Methods; 5.2 Preparation of Native Samples; 5.3 Samples for SDS Electrophoresis; 5.4 Samples for High-Resolution 2D PAGE; References; Chapter 6: Protein Detection; 6.1 Fixation; 6.2 Poststaining Methods 6.3 Prelabeling; 6.4 Difference Gel Electrophoresis (DIGE); 6.5 Imaging, Image Analysis, Spot Picking; References; Chapter 7: Blotting; 7.1 Transfer Methods; 7.2 Blotting Membranes; 7.3 Buffers for Electrophoretic Transfers; 7.4 General Staining; 7.5 Blocking; 7.6 Specific Detection; 7.7 Protein Sequencing; 7.8 Transfer Issues; 7.9 Electro-Elution of Proteins from Gels; References; Part II: Equipment and Methods; Chapter 8: Special Laboratory Equipment; Chapter 9: Consumables; Chapter 10: Chemicals; 10.1 Reagents; Method 1: PAGE of Dyes; M1.1 Sample Preparation; M1.2 Stock Solutions M1.3 Preparing the Casting Cassette; M1.4 Casting Ultra-Thin-Layer Gels; M1.5 Electrophoretic Separation; Method 2: Agarose and Immunoelectrophoresis; M2.1 Sample Preparation; M2.2 Stock Solutions; M2.3 Preparing the Gels; M2.4 Electrophoresis; M2.5 Protein Detection; References; Method 3: Titration Curve Analysis; M3.1 Sample Preparation; M3.2 Stock Solutions; M3.3 Preparing the Blank Gels; M3.4 Titration Curve Analysis; M3.5 Coomassie and Silver Staining; M3.6 Interpreting the Curves; References; Method 4: Native PAGE in Amphoteric-Buffers; M4.1 Sample Preparation; M4.2 Stock Solutions M4.3 Preparing the Empty Gels; M4.4 Electrophoresis; M4.5 Coomassie and Silver Staining; References; Method 5: Agarose IEF; M5.1 Sample Preparation; M5.2 Preparing the Agarose Gel; M5.3 Isoelectric Focusing; M5.4 Protein Detection; References; Method 6: PAGIEF in Rehydrated Gels; M6.1 Sample Preparation; M6.2 Stock Solutions; M6.3 Preparing the Blank Gels; M6.4 Isoelectric Focusing; M6.5 Coomassie and Silver Staining; M6.6 Perspectives; References; Method 7: Horizontal SDS-PAGE; M7.1 Sample Preparation; M7.2 Prelabeling with Fluorescent Dye; M7.3 Stock Solutions for Gel Preparation; M7.4 Preparing the Casting Cassette |
| Record Nr. | UNINA-9910134864103321 |
Westermeier Reiner
|
||
| Weinheim, Germany : , : Wiley-VCH Verlag, , 2016 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Electrophoresis in practice : a guide to methods and applications of DNA and protein separations / / Reiner Westermeier
| Electrophoresis in practice : a guide to methods and applications of DNA and protein separations / / Reiner Westermeier |
| Autore | Westermeier Reiner |
| Edizione | [Fifth edition.] |
| Pubbl/distr/stampa | Weinheim, Germany : , : Wiley-VCH Verlag, , 2016 |
| Descrizione fisica | 1 online resource (532 pages) |
| Disciplina | 543/.4 |
| Soggetto topico |
Electrophoresis
DNA - Separation Proteins - Separation |
| ISBN |
3-527-69516-8
3-527-69518-4 |
| Classificazione |
433.4
547.7/04572 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Title Page; Copyright; Table of Contents; Foreword; Abbreviations, Symbols, Units; Preface; German Version; English Version, Fifth Edition; Part I: Fundamentals; Chapter 1: Electrophoresis; 1.1 General; 1.2 Electrophoresis in Nonrestrictive Gels; 1.3 Electrophoresis in Restrictive Gels; References; Chapter 2: Isotachophoresis; 2.1 Migration with the Same Speed; 2.2 "Ion Train" Separation; 2.3 Zone Sharpening Effect; 2.4 Concentration Regulation Effect; 2.5 Quantitative Analysis; References; Chapter 3: Isoelectric Focusing; 3.1 Principles; 3.2 Gels for IEF; 3.3 Temperature
3.4 Controlling the pH Gradient; 3.5 Kinds of pH Gradients; 3.6 Protein Detection in IEF Gels; 3.7 Preparative Isoelectric Focusing; 3.8 Titration Curve Analysis; References; Chapter 4: High-Resolution Two-Dimensional Electrophoresis; 4.1 IEF in Immobilized pH Gradient Strips; 4.2 SDS-PAGE; 4.3 Proteomics; References; Chapter 5: Protein Sample Preparation; 5.1 Protein Quantification Methods; 5.2 Preparation of Native Samples; 5.3 Samples for SDS Electrophoresis; 5.4 Samples for High-Resolution 2D PAGE; References; Chapter 6: Protein Detection; 6.1 Fixation; 6.2 Poststaining Methods 6.3 Prelabeling; 6.4 Difference Gel Electrophoresis (DIGE); 6.5 Imaging, Image Analysis, Spot Picking; References; Chapter 7: Blotting; 7.1 Transfer Methods; 7.2 Blotting Membranes; 7.3 Buffers for Electrophoretic Transfers; 7.4 General Staining; 7.5 Blocking; 7.6 Specific Detection; 7.7 Protein Sequencing; 7.8 Transfer Issues; 7.9 Electro-Elution of Proteins from Gels; References; Part II: Equipment and Methods; Chapter 8: Special Laboratory Equipment; Chapter 9: Consumables; Chapter 10: Chemicals; 10.1 Reagents; Method 1: PAGE of Dyes; M1.1 Sample Preparation; M1.2 Stock Solutions M1.3 Preparing the Casting Cassette; M1.4 Casting Ultra-Thin-Layer Gels; M1.5 Electrophoretic Separation; Method 2: Agarose and Immunoelectrophoresis; M2.1 Sample Preparation; M2.2 Stock Solutions; M2.3 Preparing the Gels; M2.4 Electrophoresis; M2.5 Protein Detection; References; Method 3: Titration Curve Analysis; M3.1 Sample Preparation; M3.2 Stock Solutions; M3.3 Preparing the Blank Gels; M3.4 Titration Curve Analysis; M3.5 Coomassie and Silver Staining; M3.6 Interpreting the Curves; References; Method 4: Native PAGE in Amphoteric-Buffers; M4.1 Sample Preparation; M4.2 Stock Solutions M4.3 Preparing the Empty Gels; M4.4 Electrophoresis; M4.5 Coomassie and Silver Staining; References; Method 5: Agarose IEF; M5.1 Sample Preparation; M5.2 Preparing the Agarose Gel; M5.3 Isoelectric Focusing; M5.4 Protein Detection; References; Method 6: PAGIEF in Rehydrated Gels; M6.1 Sample Preparation; M6.2 Stock Solutions; M6.3 Preparing the Blank Gels; M6.4 Isoelectric Focusing; M6.5 Coomassie and Silver Staining; M6.6 Perspectives; References; Method 7: Horizontal SDS-PAGE; M7.1 Sample Preparation; M7.2 Prelabeling with Fluorescent Dye; M7.3 Stock Solutions for Gel Preparation; M7.4 Preparing the Casting Cassette |
| Record Nr. | UNINA-9910829015203321 |
|
Westermeier Reiner
|
||
| Weinheim, Germany : , : Wiley-VCH Verlag, , 2016 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
