Advanced optical flow cytometry [[electronic resource] ] : methods and disease diagnoses / / edited by Valery V. Tuchin |
Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2011 |
Descrizione fisica | 1 online resource (741 p.) |
Disciplina | 616.07582 |
Altri autori (Persone) | TuchinV. V (Valerii Viktorovich) |
Soggetto topico |
Flow cytometry - Diagnostic use
Cytometry |
Soggetto genere / forma | Electronic books. |
ISBN |
3-527-63429-0
3-527-63430-4 3-527-63428-2 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Advanced Optical Flow Cytometry; Contents; Preface; List of Contributors; 1 Perspectives in Cytometry; 1.1 Background; 1.2 Basics of Cytometry; 1.2.1 Flow Cytometry; 1.2.2 Slide-Based Cytometry; 1.3 Cytomics; 1.4 Cytometry - State of the Art; 1.4.1 Multiparametric Analyses; 1.5 Perspectives; 1.5.1 New Technologies and Methods; 1.5.1.1 Sequential Analyses; 1.5.1.2 Spectral Analyses; 1.5.1.3 Fluorescence Modifications for Analyses; 1.5.1.4 Label-Free Analyses; 1.5.2 Automation; 1.5.3 Cytometry - the Other Side; 1.6 Conclusion; References; 2 Novel Concepts and Requirements in Cytometry
2.1 Introduction2.2 Fluorescence Microscopy; 2.2.1 Light Dose; 2.2.2 Cell Systems; 2.2.3 Methods; 2.3 Fluorescence Reader Systems; 2.3.1 Cell-Based Fluorescence Screening; 2.3.2 TIR Fluorescence Reader; 2.4 Microfluidics Based on Optical Tweezers; 2.5 Conclusion; Acknowledgment; References; 3 Optical Imaging of Cells with Gold Nanoparticle Clusters as Light Scattering Contrast Agents: A Finite-Difference Time-Domain Approach to the Modeling of Flow Cytometry Configurations; 3.1 Introduction; 3.2 Fundamentals of the FDTD Method; 3.2.1 The Basic FDTD Numerical Scheme 3.2.2 Input Wave Excitation3.2.3 Uniaxial Perfectly Matched Layer Absorbing Boundary Conditions; 3.2.4 FDTD Formulation of the Light Scattering Properties from Single Cells; 3.2.5 FDTD Formulation of Optical Phase Contrast Microscopic (OPCM) Imaging; 3.3 FDTD Simulation Results of Light Scattering Patterns from Single Cells; 3.3.1 Effect of Extracellular Medium Absorption on the Light Scattering Patterns; 3.4 FDTD OPCM Nanobioimaging Simulation Results; 3.4.1 Cell Structure; 3.4.2 Optical Clearing Effect; 3.4.3 The Cell Imaging Effect of Gold Nanoparticles 3.4.3.1 A Cell with a Cluster of Gold Nanoparticles Located in the Cytoplasm3.4.3.2 A Cell with a Cluster of Gold Nanoparticles Randomly Distributed on the Surface of its Nucleus; 3.5 Conclusion; Acknowledgment; References; 4 Optics of White Blood Cells: Optical Models, Simulations, and Experiments; 4.1 Introduction; 4.1.1 White Blood Cells; 4.1.2 Particle Identification and Characterization; 4.1.3 Experimental Techniques; 4.2 Optical Models of White Blood Cells; 4.2.1 Confocal Imaging of White Blood Cells; 4.2.2 Optical Models of Mononuclear Cells; 4.2.3 Optical Models of Granular Cells 4.2.4 Refractive Indices of White Blood Cells and their Organelles4.3 Direct and Inverse Light-Scattering Problems for White Blood Cells; 4.3.1 Simulation of Light Scattering by Mononuclear Cells; 4.3.2 Simulation of Light Scattering by Granular Cells; 4.3.2.1 Granulocyte Model Without Nucleus; 4.3.2.2 Approximate Theories; 4.3.2.3 Neutrophil Model with Nucleus; 4.3.3 Inverse Light-Scattering Problem for Mononuclear Cells; 4.3.3.1 Global Optimization; 4.3.3.2 Errors of Parameter Estimates; 4.3.3.3 Theoretical Tests Based on More Complicated Model; 4.3.3.4 Sample Characterization 4.4 Experimental Measurement of Light Scattering by White Blood Cells |
Record Nr. | UNINA-9910131028303321 |
Weinheim, : Wiley-VCH, c2011 | ||
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Lo trovi qui: Univ. Federico II | ||
|
Advanced optical flow cytometry [[electronic resource] ] : methods and disease diagnoses / / edited by Valery V. Tuchin |
Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2011 |
Descrizione fisica | 1 online resource (741 p.) |
Disciplina | 616.07582 |
Altri autori (Persone) | TuchinV. V (Valerii Viktorovich) |
Soggetto topico |
Flow cytometry - Diagnostic use
Cytometry |
ISBN |
3-527-63429-0
3-527-63430-4 3-527-63428-2 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Advanced Optical Flow Cytometry; Contents; Preface; List of Contributors; 1 Perspectives in Cytometry; 1.1 Background; 1.2 Basics of Cytometry; 1.2.1 Flow Cytometry; 1.2.2 Slide-Based Cytometry; 1.3 Cytomics; 1.4 Cytometry - State of the Art; 1.4.1 Multiparametric Analyses; 1.5 Perspectives; 1.5.1 New Technologies and Methods; 1.5.1.1 Sequential Analyses; 1.5.1.2 Spectral Analyses; 1.5.1.3 Fluorescence Modifications for Analyses; 1.5.1.4 Label-Free Analyses; 1.5.2 Automation; 1.5.3 Cytometry - the Other Side; 1.6 Conclusion; References; 2 Novel Concepts and Requirements in Cytometry
2.1 Introduction2.2 Fluorescence Microscopy; 2.2.1 Light Dose; 2.2.2 Cell Systems; 2.2.3 Methods; 2.3 Fluorescence Reader Systems; 2.3.1 Cell-Based Fluorescence Screening; 2.3.2 TIR Fluorescence Reader; 2.4 Microfluidics Based on Optical Tweezers; 2.5 Conclusion; Acknowledgment; References; 3 Optical Imaging of Cells with Gold Nanoparticle Clusters as Light Scattering Contrast Agents: A Finite-Difference Time-Domain Approach to the Modeling of Flow Cytometry Configurations; 3.1 Introduction; 3.2 Fundamentals of the FDTD Method; 3.2.1 The Basic FDTD Numerical Scheme 3.2.2 Input Wave Excitation3.2.3 Uniaxial Perfectly Matched Layer Absorbing Boundary Conditions; 3.2.4 FDTD Formulation of the Light Scattering Properties from Single Cells; 3.2.5 FDTD Formulation of Optical Phase Contrast Microscopic (OPCM) Imaging; 3.3 FDTD Simulation Results of Light Scattering Patterns from Single Cells; 3.3.1 Effect of Extracellular Medium Absorption on the Light Scattering Patterns; 3.4 FDTD OPCM Nanobioimaging Simulation Results; 3.4.1 Cell Structure; 3.4.2 Optical Clearing Effect; 3.4.3 The Cell Imaging Effect of Gold Nanoparticles 3.4.3.1 A Cell with a Cluster of Gold Nanoparticles Located in the Cytoplasm3.4.3.2 A Cell with a Cluster of Gold Nanoparticles Randomly Distributed on the Surface of its Nucleus; 3.5 Conclusion; Acknowledgment; References; 4 Optics of White Blood Cells: Optical Models, Simulations, and Experiments; 4.1 Introduction; 4.1.1 White Blood Cells; 4.1.2 Particle Identification and Characterization; 4.1.3 Experimental Techniques; 4.2 Optical Models of White Blood Cells; 4.2.1 Confocal Imaging of White Blood Cells; 4.2.2 Optical Models of Mononuclear Cells; 4.2.3 Optical Models of Granular Cells 4.2.4 Refractive Indices of White Blood Cells and their Organelles4.3 Direct and Inverse Light-Scattering Problems for White Blood Cells; 4.3.1 Simulation of Light Scattering by Mononuclear Cells; 4.3.2 Simulation of Light Scattering by Granular Cells; 4.3.2.1 Granulocyte Model Without Nucleus; 4.3.2.2 Approximate Theories; 4.3.2.3 Neutrophil Model with Nucleus; 4.3.3 Inverse Light-Scattering Problem for Mononuclear Cells; 4.3.3.1 Global Optimization; 4.3.3.2 Errors of Parameter Estimates; 4.3.3.3 Theoretical Tests Based on More Complicated Model; 4.3.3.4 Sample Characterization 4.4 Experimental Measurement of Light Scattering by White Blood Cells |
Record Nr. | UNINA-9910830757003321 |
Weinheim, : Wiley-VCH, c2011 | ||
![]() | ||
Lo trovi qui: Univ. Federico II | ||
|
Advanced optical flow cytometry [[electronic resource] ] : methods and disease diagnoses / / edited by Valery V. Tuchin |
Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2011 |
Descrizione fisica | 1 online resource (741 p.) |
Disciplina | 616.07582 |
Altri autori (Persone) | TuchinV. V (Valerii Viktorovich) |
Soggetto topico |
Flow cytometry - Diagnostic use
Cytometry |
ISBN |
3-527-63429-0
3-527-63430-4 3-527-63428-2 |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Advanced Optical Flow Cytometry; Contents; Preface; List of Contributors; 1 Perspectives in Cytometry; 1.1 Background; 1.2 Basics of Cytometry; 1.2.1 Flow Cytometry; 1.2.2 Slide-Based Cytometry; 1.3 Cytomics; 1.4 Cytometry - State of the Art; 1.4.1 Multiparametric Analyses; 1.5 Perspectives; 1.5.1 New Technologies and Methods; 1.5.1.1 Sequential Analyses; 1.5.1.2 Spectral Analyses; 1.5.1.3 Fluorescence Modifications for Analyses; 1.5.1.4 Label-Free Analyses; 1.5.2 Automation; 1.5.3 Cytometry - the Other Side; 1.6 Conclusion; References; 2 Novel Concepts and Requirements in Cytometry
2.1 Introduction2.2 Fluorescence Microscopy; 2.2.1 Light Dose; 2.2.2 Cell Systems; 2.2.3 Methods; 2.3 Fluorescence Reader Systems; 2.3.1 Cell-Based Fluorescence Screening; 2.3.2 TIR Fluorescence Reader; 2.4 Microfluidics Based on Optical Tweezers; 2.5 Conclusion; Acknowledgment; References; 3 Optical Imaging of Cells with Gold Nanoparticle Clusters as Light Scattering Contrast Agents: A Finite-Difference Time-Domain Approach to the Modeling of Flow Cytometry Configurations; 3.1 Introduction; 3.2 Fundamentals of the FDTD Method; 3.2.1 The Basic FDTD Numerical Scheme 3.2.2 Input Wave Excitation3.2.3 Uniaxial Perfectly Matched Layer Absorbing Boundary Conditions; 3.2.4 FDTD Formulation of the Light Scattering Properties from Single Cells; 3.2.5 FDTD Formulation of Optical Phase Contrast Microscopic (OPCM) Imaging; 3.3 FDTD Simulation Results of Light Scattering Patterns from Single Cells; 3.3.1 Effect of Extracellular Medium Absorption on the Light Scattering Patterns; 3.4 FDTD OPCM Nanobioimaging Simulation Results; 3.4.1 Cell Structure; 3.4.2 Optical Clearing Effect; 3.4.3 The Cell Imaging Effect of Gold Nanoparticles 3.4.3.1 A Cell with a Cluster of Gold Nanoparticles Located in the Cytoplasm3.4.3.2 A Cell with a Cluster of Gold Nanoparticles Randomly Distributed on the Surface of its Nucleus; 3.5 Conclusion; Acknowledgment; References; 4 Optics of White Blood Cells: Optical Models, Simulations, and Experiments; 4.1 Introduction; 4.1.1 White Blood Cells; 4.1.2 Particle Identification and Characterization; 4.1.3 Experimental Techniques; 4.2 Optical Models of White Blood Cells; 4.2.1 Confocal Imaging of White Blood Cells; 4.2.2 Optical Models of Mononuclear Cells; 4.2.3 Optical Models of Granular Cells 4.2.4 Refractive Indices of White Blood Cells and their Organelles4.3 Direct and Inverse Light-Scattering Problems for White Blood Cells; 4.3.1 Simulation of Light Scattering by Mononuclear Cells; 4.3.2 Simulation of Light Scattering by Granular Cells; 4.3.2.1 Granulocyte Model Without Nucleus; 4.3.2.2 Approximate Theories; 4.3.2.3 Neutrophil Model with Nucleus; 4.3.3 Inverse Light-Scattering Problem for Mononuclear Cells; 4.3.3.1 Global Optimization; 4.3.3.2 Errors of Parameter Estimates; 4.3.3.3 Theoretical Tests Based on More Complicated Model; 4.3.3.4 Sample Characterization 4.4 Experimental Measurement of Light Scattering by White Blood Cells |
Record Nr. | UNINA-9910841289203321 |
Weinheim, : Wiley-VCH, c2011 | ||
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Lo trovi qui: Univ. Federico II | ||
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Population enrichment and isolation with magnetic sorting / / Joshua M. Kogot [and four others] |
Autore | Kogot Joshua Michael <1979-> |
Pubbl/distr/stampa | Adelphi, MD : , : Army Research Laboratory, , 2011 |
Descrizione fisica | 1 online resource (vi, 14 pages) : color illustrations |
Collana | ARL-TN |
Soggetto topico |
Cytometry
Magnetic separation Cell separation |
Formato | Materiale a stampa ![]() |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNINA-9910701356903321 |
Kogot Joshua Michael <1979->
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Adelphi, MD : , : Army Research Laboratory, , 2011 | ||
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Lo trovi qui: Univ. Federico II | ||
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