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Handbook of single-cell technologies / / Tuhin Subhra Santra, Fan-Gang Tseng, editors
| Handbook of single-cell technologies / / Tuhin Subhra Santra, Fan-Gang Tseng, editors |
| Pubbl/distr/stampa | Singapore : , : Springer, , [2022] |
| Descrizione fisica | 1 online resource (1091 pages) |
| Disciplina | 571.6028 |
| Soggetto topico |
Cytology - Technique
Cells - Analysis |
| ISBN | 981-10-8953-1 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Intro -- Preface -- Contents -- About the Editors -- Contributors -- Part I: Physical Methods for Single Cell Therapy and Analysis -- 1 Light-Induced Cellular Delivery and Analysis -- Introduction -- Light-Induced Intracellular Delivery from Bulk to Single-Cell Photoporation -- Direct Laser-Cell Interaction -- Indirect Laser-Cell Interaction -- Pulsed Laser Systems for Optoporation -- Nanosecond Laser -- Femtosecond Laser -- Modifications of the Method -- Applications of Single-Cell Surgery -- Conclusion -- References -- 2 Mechanoporation: Toward Single Cell Approaches -- Introduction -- Types of Mechanoporation -- Particle Bombardment -- Microinjection -- Nanoneedle AFM Tip -- Microinjection Using Microfluidic Platforms -- Parallel Delivery -- Oscillating Nanoneedle Array -- Pressure Driven Devices -- Hydrodynamic Effect -- Sonoporation -- Shear Flow Devices -- A Cone and Plate Shearing Device -- Microchannel-Based Devices -- Mechanical Confinement -- High-Throughput Parallel Mechanical Confinement Device -- Microfluidic Devices -- Constriction Channel-Based Delivery -- Cell Squeeze Platform -- DNA Transfection Via Mechanical and Electrical Cell Membrane Disruption -- Advantages and Limitations of Mechanoporation -- Future Prospects -- Summary -- References -- 3 Single-Cell Electroporation -- Introduction -- Brief History -- Operation Mechanism -- Challenges in Macroscale Electroporation -- Landscape Transformation -- Electroporation Theories -- Intracellular Molecular Transport -- Cell Membrane Resealing Dynamics -- Microscale Electroporation -- Patch-Clamp Based Techniques -- Patch-Free Techniques -- Feedback-Driven Approaches -- Conclusion and Future Outlook -- References -- 4 Microinjection for Single-Cell Analysis and Therapy -- Introduction -- Development of Microinjection -- History of Microinjection.
Types of Cargos Used for Microinjection -- Types of Host Cells Used in Microinjection -- Instruments for Microinjection -- Microinjection for Single-Cell Analysis -- Injection for Analysis of Changes in Cells -- Transgenic Animal Production -- Modern Advancements for Increasing Efficiency -- Microinjection for Single-Cell Therapy -- Intracytoplasmic Sperm Injection for Treatment of Infertility -- Gene Therapy by Injection -- Microneedles for Therapeutic Uses -- Advantages -- Disadvantages -- Future Perspective and Conclusion -- References -- Part II: Fluidic System and Integration -- 5 Single-Cell Manipulation -- Introduction -- Flow Modeling of Hydrodynamic Cell Manipulation -- Fluidic Resistance Versus Electric Resistance -- Conservation of Mass and Energy Versus Kirchhoff´s Current Law and Voltage Law -- Division of Pressure and Flow Rate Versus Division of Voltage and Current -- Examples of Microfluidic Manipulation -- Single-Cell Trapping -- Co-culture of Single Cells -- Deforming, Sorting, and Separating Single Cells -- Integration with Other Methods -- Optical Integration -- Dielectrophoretic Integration -- Magnetic Integration -- Acoustic Integration -- Conclusion -- References -- 6 Single Cell Manipulation Using Macro-scale Actuator -- Introduction -- Transfer Function of Macro-to-Micro Manipulation (Mizoue et al. 2017) -- The Driving Mechanism -- Mechanical Model -- System Identification Through Experiments -- On-Chip Transmitter for Enhancing Manipulation Speed (Monzawa et al. 2015) -- Advantages of Using an Actuation Transmitter -- Mechanical Model and Theoretical Basis -- Experimental Validation on Manipulation with an Actuation Transmitter -- Recent Works and Applications -- Summary -- References -- 7 Inertial Microfluidics for Single-Cell Manipulation and Analysis -- Introduction -- Underlying Physics -- Inertial Migration. Dean Flow -- Viscoelasticity -- Guidelines for Designing Spiral Inertial Microfluidics -- Dimensionless Numbers -- Design Guidelines -- Cross-Sectional Dimensions -- Channel Length (Loop Number) -- Other Structural Parameters -- Other Functional Units -- Operational Parameters -- Improved Understandings on Spiral Inertial Microfluidics -- Application Guidelines for Spiral Inertial Microfluidics -- Focusing/Ordering -- Separation -- Concentration/Microfiltration -- Conclusion and Future Perspective -- References -- 8 Digital Microfluidics for Single Cell Manipulation and Analysis -- Introduction -- DMF Systems -- DMF Technology -- Electrowetting-on-Dielectric -- Electrodewetting -- Liquid Dielectrophoresis -- Dielectrophoresis -- Optoelectrowetting -- Optoelectronic Tweezers -- Magnetic Force -- Fabrication of DMF -- DMF Manipulation and Analysis of a Single Cell -- Adherent Cell -- Suspension Cell -- Long-Term Culture -- Related Applications of DMF -- Cell Sorting and Concentrating -- 3D Cell Culture -- Diagnosis and Clinical Application -- Conclusions and Future Outlook -- References -- 9 Single-Cell Separation -- Introduction -- Conventional Cell Separation Techniques -- Centrifugation -- Fluorescence-Activated Cell Sorting -- Magnetic-Activated Cell Sorting -- Laser Capture Microdissection -- Manual Cell Picking -- Microfluidic Single-Cell Separation Techniques -- Microfluidic Passive Separation Techniques -- Filter-Based Separation -- Deterministic Lateral Displacement -- Hydrodynamic Separation -- Non-inertial Hydrodynamic Separation -- Inertial Hydrodynamic Separation -- Microfluidic Active Separation Techniques -- Dielectrophoresis (DEP) -- Magnetic Separation -- Acoustophoresis -- Affinity-Based Separation -- Comparison Between Different Microfluidic Separation Techniques -- Conclusion -- References. 10 Technologies for Automated Single Cell Isolation -- Introduction -- Cell Samples -- Size and Morphology of Cells -- Sample Preparation: Single-Cell Suspensions -- Basic Considerations, Definitions, and Classifications for Single-Cell Isolation -- Automated Single-Cell Isolation Technologies -- Limiting Dilution -- Suitability for Microbial Cells -- Fluorescence-Activated Cell Sorting -- Suitability for Microbial Cells -- Single-Cell Dispensing (SCD) -- Suitability for Microbial Cells -- Microfluidic Single-Cell Isolation -- Hydrodynamic Trapping -- Vertical Trapping in Nanowells -- Suitability for Microbial Cells -- Droplet Microfluidics -- Single-Cell Manipulation (on Microfluidic Chips) by Directed External Forces -- Optical Tweezers (OT) -- Dielectrophoresis (DEP) -- Suitability for Microbial Cells -- Automated Micromanipulators -- Discussion and Conclusion -- References -- 11 Dual-Well Microfluidic Technique for Single Cell Isolation and Long-Term Clonal Culture -- Introduction -- Applications of Monoclonal Cell Culture -- Overview of Single Cell Isolation Methods for Clonal Culture -- The Design Concept of the Dual-Well Technique -- Fabrication of Dual-Well Device -- Fabrication of Master Molds by Photolithography Technology -- Molding PDMS Device with the Master Molds -- Dual-Well Device Preparation for Single Cell Isolation -- Cell Suspension Preparation for Single Cell Isolation with Dual-Well Device -- Single Cell Isolation and Clonal Culture with Dual-Well Device -- Culture Medium Replacement of the Dual-Well Device -- Discussion -- The Effect of Capture Well Depth on Single Cell Efficiency -- The Effect of Washing Flow Rate on Single Cell Efficiency -- The Effect of Cell Type on Single Cell Capture Efficiency -- The Effect of Device Flipping on Cell Transfer Efficiency. Single Cell-Derived Clonal Colonies and Stem Cell Differentiation in the Microwells of the Dual-Well Device -- Conclusion -- References -- 12 Single-Cell Cultivation Utilizing Microfluidic Systems -- Introduction -- Purpose of Single-Cell Cultivation -- Morphology -- Proliferation and Differentiation -- Migration -- Genomics, Transcriptomics, Proteomics, and Metabolomics -- Epigenomics -- Single-Cell Cultivation in Microfluidic Devices -- Cell Coculture -- Neural Coculture -- Coculture of Tumor Cells to Neighboring Cells -- Coculture of Neurons and Cancer Cells -- Molecule-Induced Cellular Behaviors -- Molecular Cue-Guided Neuron -- Molecule and Immune Cell Interaction -- Molecule and Microbe Interaction -- Regeneration -- Axotomy -- Stem Cell-Based Regeneration -- Further Limitations and Future Prospects -- Conclusion -- References -- 13 Integrated Microwell Array Technologies for Single Cell Analysis -- Introduction -- Microfabrication of a Microwell Array -- Material and Design Considerations -- Soft Photolithography for Fast Prototyping of Microwell Arrays -- Hydrophilic-in-Hydrophobic Microwells -- Polyethylene Glycol Microwells for Reduced Non-specific Adsorption -- Single Cell Docking -- Docking Strategies: Manual Administration of Cells -- Docking Strategies: Microfluidics-Assisted Administration of Cells -- Continuous Flow Microfluidics -- Digital Microfluidics -- Single Cell Manipulation -- Optical Manipulation -- Magnetic Manipulation -- Mechanical Manipulation -- Electric Manipulation -- Applications -- Single Cell Drug Screening -- Single Cell Omics -- Detection of Single Cell Secreted Products -- Other Applications -- Conclusion -- References -- 14 Micro- and Nanopore Technologies for Single-Cell Analysis -- Coulter Principle -- A History Behind the Invention of Coulter Principle -- Single-Cell Counting Mechanism. Single-Cell Analysis with Conventional Coulter Counters. |
| Record Nr. | UNINA-9910508470903321 |
| Singapore : , : Springer, , [2022] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Single cell analysis [[electronic resource] ] : technologies and applications / / edited by Dario Anselmetti
| Single cell analysis [[electronic resource] ] : technologies and applications / / edited by Dario Anselmetti |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2009 |
| Descrizione fisica | 1 online resource (286 p.) |
| Disciplina | 571.6 |
| Altri autori (Persone) | AnselmettiD |
| Soggetto topico |
Cells - Analysis
Cytology |
| ISBN |
1-282-46084-6
9786612460845 3-527-62665-4 3-527-62664-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Single Cell Analysis: Technologies and Applications; Contents; Foreword; Preface; List of Contributors; Part I Single Cell Analysis: Imaging; 1 Single Molecule Fluorescence Monitoring in Eukaryotic Cells: Intranuclear Dynamics of Splicing Factors; 1.1 Motivation; 1.2 Experimental Approach; 1.3 Single Particle Tracking within Living Cells; 1.4 Pre-Messenger RNA Splicing; 1.5 Intranuclear Splicing Factor Tracking; 1.6 Intranuclear U1 snRNP Splicing Factor Binding; 1.7 Events in Speckles; 1.8 Intranuclear U1 snRNP Mobility; 1.9 Perspectives of Single Molecule Microscopy; References
2 Gene Classification and Quantitative Analysis of Gene Regulation in Bacteria using Single Cell Atomic Force Microscopy and Single Molecule Force Spectroscopy2.1 Introduction; 2.2 AFM on Paracrystalline Cell Surface Layers of C. glutamicum: Protein Sequence Information and Morphology; 2.3 Imaging of Living C. glutamicum Cells with Molecular Resolution: Genes, Transcriptional Regulation and Morphology; 2.4 Single Molecule Force Spectroscopy on Specific Protein-DNA Complexes: Transcriptional Regulation in S. meliloti 2.5 Effector-Induced Protein-DNA Binding on the Single Molecule Level: Quorum Sensing in S. meliloti2.6 Conclusion; References; 3 Cellular Cryo-Electron Tomography (CET): Towards a Voyage to the Inner Space of Cells; 3.1 Introduction; 3.2 Tomography with the Electron Microscope - a Practical Perspective; 3.2.1 Sample Preparation; 3.2.2 Instrumental and Technical Requirements; 3.2.3 Alignment, Reconstruction and Visualization; 3.3 Molecular Interpretation of Cellular Tomograms; 3.4 Outlook: The Future is Bright; References; Part II Single Cell Analysis: Technologies; 4 Single Cell Proteomics 4.1 Introduction4.2 The Challenge; 4.3 Single Cell Proteomics: Mass Spectrometry; 4.4 Single Cell Separations; 4.5 Ultrasensitive Protein Analysis: Capillary Electrophoresis with Laser-Induced Fluorescence Detection; 4.6 Capillary Sieving Electrophoresis of Proteins from a Single Cancer Cell; 4.7 Cell Cycle-dependent Single Cell Capillary Sieving Electrophoresis; 4.8 Tentative Identification of Proteins in Single Cell Electropherograms; 4.9 Capillary Micellar and Submicellar Separation of Proteins from a Single Cell; 4.10 Two-Dimensional Capillary Electrophoresis of Proteins in a Single Cell 4.11 Single Copy Detection of Specific Proteins in Single Cells4.12 Conclusion; References; 5 Protein Analysis of Single Cells in Microfluidic Format; 5.1 Introduction; 5.2 Microfluidic Single Cell Analysis Concept; 5.2.1 Single Cell Selection and Trapping; 5.2.2 Single Cell Lysis; 5.3 Single Cell Electrophoretic Separation and Detection of Proteins; 5.3.1 Label-Based Fluorescence Detection; 5.3.2 Label-Free Fluorescence Detection; 5.3.2.1 UV-LIF in Quartz Microfluidic Devices; 5.3.2.2 UV-LIF in PDMS Microfluidic Devices; 5.3.2.3 Single Cell UV-LIF Electrophoretic Analysis 5.4 Future Directions in Single Cell Analysis |
| Record Nr. | UNINA-9910140015103321 |
| Weinheim, : Wiley-VCH, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Single cell analysis [[electronic resource] ] : technologies and applications / / edited by Dario Anselmetti
| Single cell analysis [[electronic resource] ] : technologies and applications / / edited by Dario Anselmetti |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2009 |
| Descrizione fisica | 1 online resource (286 p.) |
| Disciplina | 571.6 |
| Altri autori (Persone) | AnselmettiD |
| Soggetto topico |
Cells - Analysis
Cytology |
| ISBN |
1-282-46084-6
9786612460845 3-527-62665-4 3-527-62664-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Single Cell Analysis: Technologies and Applications; Contents; Foreword; Preface; List of Contributors; Part I Single Cell Analysis: Imaging; 1 Single Molecule Fluorescence Monitoring in Eukaryotic Cells: Intranuclear Dynamics of Splicing Factors; 1.1 Motivation; 1.2 Experimental Approach; 1.3 Single Particle Tracking within Living Cells; 1.4 Pre-Messenger RNA Splicing; 1.5 Intranuclear Splicing Factor Tracking; 1.6 Intranuclear U1 snRNP Splicing Factor Binding; 1.7 Events in Speckles; 1.8 Intranuclear U1 snRNP Mobility; 1.9 Perspectives of Single Molecule Microscopy; References
2 Gene Classification and Quantitative Analysis of Gene Regulation in Bacteria using Single Cell Atomic Force Microscopy and Single Molecule Force Spectroscopy2.1 Introduction; 2.2 AFM on Paracrystalline Cell Surface Layers of C. glutamicum: Protein Sequence Information and Morphology; 2.3 Imaging of Living C. glutamicum Cells with Molecular Resolution: Genes, Transcriptional Regulation and Morphology; 2.4 Single Molecule Force Spectroscopy on Specific Protein-DNA Complexes: Transcriptional Regulation in S. meliloti 2.5 Effector-Induced Protein-DNA Binding on the Single Molecule Level: Quorum Sensing in S. meliloti2.6 Conclusion; References; 3 Cellular Cryo-Electron Tomography (CET): Towards a Voyage to the Inner Space of Cells; 3.1 Introduction; 3.2 Tomography with the Electron Microscope - a Practical Perspective; 3.2.1 Sample Preparation; 3.2.2 Instrumental and Technical Requirements; 3.2.3 Alignment, Reconstruction and Visualization; 3.3 Molecular Interpretation of Cellular Tomograms; 3.4 Outlook: The Future is Bright; References; Part II Single Cell Analysis: Technologies; 4 Single Cell Proteomics 4.1 Introduction4.2 The Challenge; 4.3 Single Cell Proteomics: Mass Spectrometry; 4.4 Single Cell Separations; 4.5 Ultrasensitive Protein Analysis: Capillary Electrophoresis with Laser-Induced Fluorescence Detection; 4.6 Capillary Sieving Electrophoresis of Proteins from a Single Cancer Cell; 4.7 Cell Cycle-dependent Single Cell Capillary Sieving Electrophoresis; 4.8 Tentative Identification of Proteins in Single Cell Electropherograms; 4.9 Capillary Micellar and Submicellar Separation of Proteins from a Single Cell; 4.10 Two-Dimensional Capillary Electrophoresis of Proteins in a Single Cell 4.11 Single Copy Detection of Specific Proteins in Single Cells4.12 Conclusion; References; 5 Protein Analysis of Single Cells in Microfluidic Format; 5.1 Introduction; 5.2 Microfluidic Single Cell Analysis Concept; 5.2.1 Single Cell Selection and Trapping; 5.2.2 Single Cell Lysis; 5.3 Single Cell Electrophoretic Separation and Detection of Proteins; 5.3.1 Label-Based Fluorescence Detection; 5.3.2 Label-Free Fluorescence Detection; 5.3.2.1 UV-LIF in Quartz Microfluidic Devices; 5.3.2.2 UV-LIF in PDMS Microfluidic Devices; 5.3.2.3 Single Cell UV-LIF Electrophoretic Analysis 5.4 Future Directions in Single Cell Analysis |
| Record Nr. | UNINA-9910830701603321 |
| Weinheim, : Wiley-VCH, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Single cell analysis : technologies and applications / / edited by Dario Anselmetti
| Single cell analysis : technologies and applications / / edited by Dario Anselmetti |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2009 |
| Descrizione fisica | 1 online resource (286 p.) |
| Disciplina | 571.6 |
| Altri autori (Persone) | AnselmettiD |
| Soggetto topico |
Cells - Analysis
Cytology |
| ISBN |
9786612460845
9781282460843 1282460846 9783527626656 3527626654 9783527626649 3527626646 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Single Cell Analysis: Technologies and Applications; Contents; Foreword; Preface; List of Contributors; Part I Single Cell Analysis: Imaging; 1 Single Molecule Fluorescence Monitoring in Eukaryotic Cells: Intranuclear Dynamics of Splicing Factors; 1.1 Motivation; 1.2 Experimental Approach; 1.3 Single Particle Tracking within Living Cells; 1.4 Pre-Messenger RNA Splicing; 1.5 Intranuclear Splicing Factor Tracking; 1.6 Intranuclear U1 snRNP Splicing Factor Binding; 1.7 Events in Speckles; 1.8 Intranuclear U1 snRNP Mobility; 1.9 Perspectives of Single Molecule Microscopy; References
2 Gene Classification and Quantitative Analysis of Gene Regulation in Bacteria using Single Cell Atomic Force Microscopy and Single Molecule Force Spectroscopy2.1 Introduction; 2.2 AFM on Paracrystalline Cell Surface Layers of C. glutamicum: Protein Sequence Information and Morphology; 2.3 Imaging of Living C. glutamicum Cells with Molecular Resolution: Genes, Transcriptional Regulation and Morphology; 2.4 Single Molecule Force Spectroscopy on Specific Protein-DNA Complexes: Transcriptional Regulation in S. meliloti 2.5 Effector-Induced Protein-DNA Binding on the Single Molecule Level: Quorum Sensing in S. meliloti2.6 Conclusion; References; 3 Cellular Cryo-Electron Tomography (CET): Towards a Voyage to the Inner Space of Cells; 3.1 Introduction; 3.2 Tomography with the Electron Microscope - a Practical Perspective; 3.2.1 Sample Preparation; 3.2.2 Instrumental and Technical Requirements; 3.2.3 Alignment, Reconstruction and Visualization; 3.3 Molecular Interpretation of Cellular Tomograms; 3.4 Outlook: The Future is Bright; References; Part II Single Cell Analysis: Technologies; 4 Single Cell Proteomics 4.1 Introduction4.2 The Challenge; 4.3 Single Cell Proteomics: Mass Spectrometry; 4.4 Single Cell Separations; 4.5 Ultrasensitive Protein Analysis: Capillary Electrophoresis with Laser-Induced Fluorescence Detection; 4.6 Capillary Sieving Electrophoresis of Proteins from a Single Cancer Cell; 4.7 Cell Cycle-dependent Single Cell Capillary Sieving Electrophoresis; 4.8 Tentative Identification of Proteins in Single Cell Electropherograms; 4.9 Capillary Micellar and Submicellar Separation of Proteins from a Single Cell; 4.10 Two-Dimensional Capillary Electrophoresis of Proteins in a Single Cell 4.11 Single Copy Detection of Specific Proteins in Single Cells4.12 Conclusion; References; 5 Protein Analysis of Single Cells in Microfluidic Format; 5.1 Introduction; 5.2 Microfluidic Single Cell Analysis Concept; 5.2.1 Single Cell Selection and Trapping; 5.2.2 Single Cell Lysis; 5.3 Single Cell Electrophoretic Separation and Detection of Proteins; 5.3.1 Label-Based Fluorescence Detection; 5.3.2 Label-Free Fluorescence Detection; 5.3.2.1 UV-LIF in Quartz Microfluidic Devices; 5.3.2.2 UV-LIF in PDMS Microfluidic Devices; 5.3.2.3 Single Cell UV-LIF Electrophoretic Analysis 5.4 Future Directions in Single Cell Analysis |
| Record Nr. | UNINA-9911020245803321 |
| Weinheim, : Wiley-VCH, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Soggetto topico
-
Cells - Analysis
(4)
- Cytology (3)
- Cytology - Technique (1)