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Biblioteca

MARC Lista (tabellare)
Capillary electrochromatography [[electronic resource] /] / edited by Keith D. Bartle, Peter Myers
Capillary electrochromatography [[electronic resource] /] / edited by Keith D. Bartle, Peter Myers
Pubbl/distr/stampa Cambridge, : Royal Society of Chemistry, 2001
Descrizione fisica 1 online resource (165 p.)
Disciplina 543.0894
Altri autori (Persone) BartleKeith D
MyersPeter
Collana RSC chromatography monographs
Soggetto topico Capillary electrophoresis
Capillary liquid chromatography
Soggetto genere / forma Electronic books.
ISBN 1-84755-030-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto BK9780854045303-FX001; 85ba00; 85ba00a; 85ba00b; 85ba00c; 85ba01; 85ba02; 85ba03; 85ba04; 85ba05; 85ba06; 85ba07; 85ba08; 85ba-ind
Record Nr. UNINA-9910455251403321
Cambridge, : Royal Society of Chemistry, 2001
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrochromatography [[electronic resource] /] / edited by Keith D. Bartle, Peter Myers
Capillary electrochromatography [[electronic resource] /] / edited by Keith D. Bartle, Peter Myers
Pubbl/distr/stampa Cambridge, : Royal Society of Chemistry, 2001
Descrizione fisica 1 online resource (165 p.)
Disciplina 543.0894
Altri autori (Persone) BartleKeith D
MyersPeter
Collana RSC chromatography monographs
Soggetto topico Capillary electrophoresis
Capillary liquid chromatography
ISBN 1-84755-030-4
Classificazione JE 92
VG 7500
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto BK9780854045303-FX001; 85ba00; 85ba00a; 85ba00b; 85ba00c; 85ba01; 85ba02; 85ba03; 85ba04; 85ba05; 85ba06; 85ba07; 85ba08; 85ba-ind
Record Nr. UNINA-9910778306003321
Cambridge, : Royal Society of Chemistry, 2001
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrochromatography [[electronic resource] /] / edited by Keith D. Bartle, Peter Myers
Capillary electrochromatography [[electronic resource] /] / edited by Keith D. Bartle, Peter Myers
Edizione [1st ed.]
Pubbl/distr/stampa Cambridge, : Royal Society of Chemistry, 2001
Descrizione fisica 1 online resource (165 p.)
Disciplina 543.0894
Altri autori (Persone) BartleKeith D
MyersPeter
Collana RSC chromatography monographs
Soggetto topico Capillary electrophoresis
Capillary liquid chromatography
ISBN 1-84755-030-4
Classificazione JE 92
VG 7500
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto BK9780854045303-FX001; 85ba00; 85ba00a; 85ba00b; 85ba00c; 85ba01; 85ba02; 85ba03; 85ba04; 85ba05; 85ba06; 85ba07; 85ba08; 85ba-ind
Record Nr. UNINA-9910826960803321
Cambridge, : Royal Society of Chemistry, 2001
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrophoresis - mass spectrometry (CE-MS) : principles and applications / / edited by Gerhardus de Jong
Capillary electrophoresis - mass spectrometry (CE-MS) : principles and applications / / edited by Gerhardus de Jong
Pubbl/distr/stampa Weinheim, Germany : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2016
Descrizione fisica 1 online resource (421 p.)
Disciplina 543.0871
Soggetto topico Capillary electrophoresis
Mass spectrometry
ISBN 3-527-69383-1
3-527-69381-5
3-527-69380-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910134854103321
Weinheim, Germany : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2016
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrophoresis -- mass spectrometry for proteomics and metabolomics : principles and applications / / edited by Rawi Ramautar and David D. Y. Chen
Capillary electrophoresis -- mass spectrometry for proteomics and metabolomics : principles and applications / / edited by Rawi Ramautar and David D. Y. Chen
Pubbl/distr/stampa Hoboken, New Jersey : , : John Wiley & Sons, , [2023]
Descrizione fisica 1 online resource (403 pages)
Disciplina 541.372
Soggetto topico Capillary electrophoresis
ISBN 3-527-83309-9
3-527-83308-0
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover -- Title Page -- Copyright -- Contents -- Preface -- Chapter 1 Capillary Electrophoresis-Mass Spectrometry Interfacing: Principles and Recent Developments -- 1.1 Introduction -- 1.2 General Considerations of CE-ESI-MS -- 1.2.1 Electrospray Ionization -- 1.2.2 Electrical Circuit in CE-ESI-MS -- 1.2.3 CE Modes and Conditions in CE-MS -- 1.3 Sheath Liquid Interfaces -- 1.3.1 Coaxial Sheath‐Flow ESI-MS Interface -- 1.3.2 Nanoflow Sheath Liquid ESI-MS Interface -- 1.4 Sheathless Interfaces -- 1.4.1 Porous‐Tip Interface -- 1.4.2 Other Sheathless Interfaces -- 1.5 Other CE-ESI-MS Interfaces -- 1.5.1 Liquid Junction -- 1.5.2 Interface‐Free CE-MS -- 1.6 Microchip Electrophoresis-MS Interfaces -- 1.7 Alternative Ionization Techniques for CE-MS -- 1.7.1 CE and MCE Combined with MALDI-MS -- 1.7.2 CE-ICP-MS -- 1.8 Concluding Remarks and Outlook -- List of Abbreviations -- References -- Chapter 2 Data Analysis Strategies in CE-MS for Metabolomics -- 2.1 Introduction -- 2.2 The Annotation Challenge in CE-MS‐Based Untargeted Metabolomics -- 2.2.1 Peak Picking in CE-MS Metabolomics -- 2.2.2 Alignment Approaches -- 2.2.3 μeff Transformation -- 2.2.3.1 Historical Evolution and Current State -- 2.2.3.2 Quantitative Aspects of the Use of Mobilograms -- 2.2.3.3 Technical Considerations of the μeff Transformation -- 2.2.4 Reproducibility and Exchange of μeff Information Using Libraries -- 2.2.5 Interlaboratory Reproducibility -- 2.2.6 ROMANCE -- 2.2.7 Ion Mobility -- 2.3 Data Pretreatment -- 2.3.1 Area Normalization -- 2.3.2 Analytical Quality Monitoring -- 2.3.3 Data Filtering -- 2.4 Data Treatment -- 2.5 Concluding Remarks -- Acknowledgment -- References -- Chapter 3 Data‐Processing Workflow for Relative Quantification from Label‐Free and Isobaric Labeling‐Based Untargeted Shotgun Proteomics: From Database Search to Differential Expression Analysis.
3.1 Introduction -- 3.2 Spectra Acquisition and Database Search -- 3.2.1 Parameters Affecting Sequence Database Search -- 3.2.1.1 Database Selection -- 3.2.1.2 Enzyme Specificity and Missed Cleavages -- 3.2.1.3 Posttranslational Modifications -- 3.2.1.4 Precursor Mass Tolerance -- 3.2.2 Target-Decoy Search Strategy for False Discovery Rate Estimation -- 3.2.3 Database Search Engines -- 3.2.3.1 SEQUEST -- 3.2.3.2 Mascot -- 3.2.3.3 Multiple Search Engines -- 3.2.4 Protein Inference -- 3.3 Relative Protein Quantification -- 3.3.1 Filtering -- 3.3.2 Missing Value Imputation -- 3.3.2.1 Imputation Methods -- 3.3.2.2 Comparative Studies -- 3.3.2.3 Normalization -- 3.3.3 Summarization -- 3.3.4 Differential Expression Analysis -- 3.4 Conclusions -- References -- Chapter 4 Data Processing in Metabolomics Capillary Electrophoresis-Mass Spectrometry -- 4.1 Data Extraction and the Interpretation of the Extracted Data -- 4.2 Data Preprocessing -- 4.2.1 Handling Missing Values -- 4.2.2 Normalization -- 4.2.3 Transformation -- 4.2.4 Scaling -- 4.3 Statistical Analysis -- 4.3.1 Two‐Sample T‐Test -- 4.3.2 Principal Component Analysis (PCA) -- 4.3.3 Partial Least‐Squares Discriminant Analysis (PLS-DA) -- 4.3.4 Support Vector Machine -- 4.3.5 Logistic Regression -- 4.3.6 Random Forest Model -- 4.3.7 Evaluation of Classification Models -- 4.4 Metabolite Identification -- References -- Chapter 5 Utility and Advances of Capillary Electrophoresis-Mass Spectrometry for Metabolomics -- 5.1 Introduction -- 5.2 Technological Developments -- 5.2.1 Improving Sensitivity -- 5.2.2 Increasing Metabolome Coverage -- 5.2.3 Increasing Annotation Capacity -- 5.2.4 Tackling Anionic Profiling -- 5.2.5 Increasing Sample Throughput -- 5.3 Applications -- 5.3.1 Biomedical Samples -- 5.3.2 Microbial Extracts -- 5.3.3 Plants -- 5.4 Concluding Remarks -- Acknowledgment -- Conflict of Interest.
References -- Chapter 6 Comprehensive Lipid Profiling by Multisegment Injection-Nonaqueous Capillary Electrophoresis-Mass Spectrometry: Expanding Coverage Beyond Hydrophilic Metabolites -- 6.1 The Early Origins of Lipidomics -- 6.2 Major Instrumental Platforms in Lipidomics: The Role of Separation Science -- 6.3 NACE-MS: An Emerging Separation Platform for Lipidomics? -- 6.4 Multiplexed Separations for Fatty Acids by MSI-NACE-MS -- 6.5 Comprehensive Lipid Profiling Strategies by MSI-NACE-MS -- 6.6 Future Perspectives and Summary -- References -- Chapter 7 Strategies for Identification of Modified Amino Acids with CE-MS in Metabolomics -- 7.1 Introduction -- 7.1.1 Post‐Translational Modifications and Modified Amino Acids -- 7.1.2 Modified Amino Acids as Biomarkers of Pathologies -- 7.2 Methods for the Detection of Modified Amino Acids -- 7.3 Capillary Electrophoresis Coupled to Mass Spectrometry to Analyze Modified Amino Acids -- 7.3.1 Applications of CE-MS for Analysis of Modified Amino Acids -- 7.4 Recent Developments to Enhance and Facilitate the Annotation Process in CE-MS -- Acknowledgments -- References -- Chapter 8 CE-MS Approaches for Single‐Cell Metabolomics -- 8.1 Introduction -- 8.2 Techniques for Single‐Cell Metabolome Analysis -- 8.2.1 Highly Sensitive CE-MS Interfacing -- 8.2.2 Online Sample Preconcentration Techniques -- 8.2.3 Isolation of Single Cells -- 8.3 Application to Single‐Cell Metabolome Analysis -- 8.3.1 Metabolome Analysis of Large Single Cells with Highly Sensitive Interface -- 8.3.2 Single‐Cell Metabolome Analysis with Highly Sensitive Interface and OSP Method -- 8.3.3 Single‐Cell Metabolome Analysis by Online Sampling and Highly Sensitive Interface -- 8.4 Conclusions -- References -- Chapter 9 CE-MS Approaches for Peptidomics -- 9.1 Introduction -- 9.2 Sample Preparation -- 9.3 CE-MS -- 9.3.1 Basic Characterization.
9.3.2 Peptide Identification -- 9.3.3 Peptide Quantitation -- 9.4 Applications -- 9.4.1 Search, Identification, and Determination of Biomarkers -- 9.4.2 Food Peptidomics -- 9.4.3 Other Applications -- 9.5 Conclusions -- Acknowledgments -- List of Abbreviations -- References -- Chapter 10 Capillary Zone Electrophoresis-Mass Spectrometry for Top‐Down Proteomics: Technological Development and Biological Applications -- 10.1 Introduction -- 10.2 Technological Development -- 10.2.1 CE-MS Interface -- 10.2.2 Capillary Coating -- 10.2.3 Sample Loading Capacity and Separation Window -- 10.2.4 Coupling Novel Gas‐Phase Fragmentation Techniques to CZE-MS/MS for TDP -- 10.2.5 Electrophoretic Mobility Prediction of Proteoforms -- 10.3 Applications of CZE-MS‐Based TDP -- 10.3.1 Delineation of Proteoforms of Complex Proteomes, Disease Biomarkers, and Biopharmaceuticals -- 10.3.2 CZE-MS for Native TDP -- 10.4 Conclusions and Perspectives -- Acknowledgments -- References -- Chapter 11 CE-MS Methods for the Characterization of Monoclonal Antibodies -- 11.1 Introduction -- 11.2 mAb Characterization Approaches -- 11.3 Applications -- 11.3.1 Primary Structure Characterization of Monoclonal Antibodies -- 11.3.1.1 Analytical Workflow -- 11.3.1.2 Amino Acid Sequence Characterization Using CE-MS(/MS) -- 11.3.1.3 PTMs Characterization and Relative Quantification Using CE-MS(/MS) -- 11.3.1.4 Glycosylation Determination Using CE-MS(/MS) -- 11.3.2 Middle‐Up/Middle‐Down Analysis -- 11.3.2.1 Analytical Workflow -- 11.3.2.2 mAb Analysis Using Middle‐Up Strategy -- 11.3.2.3 mAb Analysis Using Middle-Down Strategy -- 11.3.3 Intact Analysis -- 11.3.3.1 Analytical Workflow -- 11.3.3.2 mAb Analysis in Denaturing Conditions Using CE-MS -- 11.3.3.3 mAb Analysis in Native Conditions Using CE-MS -- 11.3.4 Automation: Future of CE-MS for mAb Analysis -- 11.4 Conclusion -- References.
Chapter 12 CE and CE-MS Approaches for Glycan Analysis -- 12.1 The Importance of Glycosylation -- 12.2 Capillary Electrophoresis in Analytical Glycomics -- 12.2.1 Sample Preparation for CE‐Based Glycan Analysis -- 12.2.2 Analysis of Oligosaccharides from Biological Samples -- 12.2.3 Structural Elucidation of Carbohydrates: The GU Value Approach -- 12.2.4 LIF Sensitivity and Quantification -- 12.3 CE-MS of Oligosaccharides -- 12.3.1 Major Parameters to Set the ESI for CE-MS of Derivatized Oligosaccharides -- 12.3.2 CE-MS for Quantitative Carbohydrate Analysis -- 12.3.3 CE-MS for Glycomic Studies -- 12.4 Conclusions and Future Prospective -- Acknowledgment -- References -- Chapter 13 CE-MS Approaches for Glyco(proteo)mic Analysis -- 13.1 Introduction -- 13.1.1 N‐Linked Glycosylation -- 13.1.2 O‐Linked Glycosylation -- 13.1.3 Glycosylation Workflows -- 13.1.4 Analytical Approaches -- 13.1.5 CE-MS Interfaces -- 13.2 Glycan Analysis by CE-MS -- 13.2.1 Glycan Derivatization Strategies -- 13.3 Glycopeptide Analysis -- 13.3.1 Sample Treatment -- 13.3.2 Separation Conditions -- 13.3.3 Applications -- 13.4 Protein Analysis -- 13.4.1 Sample Treatment -- 13.4.2 Separation Conditions -- 13.4.3 Applications -- 13.5 Conclusions and Outlook -- Acknowledgments -- List of Abbreviations -- References -- Index -- EULA.
Record Nr. UNINA-9910644087903321
Hoboken, New Jersey : , : John Wiley & Sons, , [2023]
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrophoresis -- mass spectrometry for proteomics and metabolomics : principles and applications / / edited by Rawi Ramautar and David D. Y. Chen
Capillary electrophoresis -- mass spectrometry for proteomics and metabolomics : principles and applications / / edited by Rawi Ramautar and David D. Y. Chen
Pubbl/distr/stampa Hoboken, New Jersey : , : John Wiley & Sons, , [2023]
Descrizione fisica 1 online resource (403 pages)
Disciplina 541.372
Soggetto topico Capillary electrophoresis
ISBN 3-527-83309-9
3-527-83308-0
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover -- Title Page -- Copyright -- Contents -- Preface -- Chapter 1 Capillary Electrophoresis-Mass Spectrometry Interfacing: Principles and Recent Developments -- 1.1 Introduction -- 1.2 General Considerations of CE-ESI-MS -- 1.2.1 Electrospray Ionization -- 1.2.2 Electrical Circuit in CE-ESI-MS -- 1.2.3 CE Modes and Conditions in CE-MS -- 1.3 Sheath Liquid Interfaces -- 1.3.1 Coaxial Sheath‐Flow ESI-MS Interface -- 1.3.2 Nanoflow Sheath Liquid ESI-MS Interface -- 1.4 Sheathless Interfaces -- 1.4.1 Porous‐Tip Interface -- 1.4.2 Other Sheathless Interfaces -- 1.5 Other CE-ESI-MS Interfaces -- 1.5.1 Liquid Junction -- 1.5.2 Interface‐Free CE-MS -- 1.6 Microchip Electrophoresis-MS Interfaces -- 1.7 Alternative Ionization Techniques for CE-MS -- 1.7.1 CE and MCE Combined with MALDI-MS -- 1.7.2 CE-ICP-MS -- 1.8 Concluding Remarks and Outlook -- List of Abbreviations -- References -- Chapter 2 Data Analysis Strategies in CE-MS for Metabolomics -- 2.1 Introduction -- 2.2 The Annotation Challenge in CE-MS‐Based Untargeted Metabolomics -- 2.2.1 Peak Picking in CE-MS Metabolomics -- 2.2.2 Alignment Approaches -- 2.2.3 μeff Transformation -- 2.2.3.1 Historical Evolution and Current State -- 2.2.3.2 Quantitative Aspects of the Use of Mobilograms -- 2.2.3.3 Technical Considerations of the μeff Transformation -- 2.2.4 Reproducibility and Exchange of μeff Information Using Libraries -- 2.2.5 Interlaboratory Reproducibility -- 2.2.6 ROMANCE -- 2.2.7 Ion Mobility -- 2.3 Data Pretreatment -- 2.3.1 Area Normalization -- 2.3.2 Analytical Quality Monitoring -- 2.3.3 Data Filtering -- 2.4 Data Treatment -- 2.5 Concluding Remarks -- Acknowledgment -- References -- Chapter 3 Data‐Processing Workflow for Relative Quantification from Label‐Free and Isobaric Labeling‐Based Untargeted Shotgun Proteomics: From Database Search to Differential Expression Analysis.
3.1 Introduction -- 3.2 Spectra Acquisition and Database Search -- 3.2.1 Parameters Affecting Sequence Database Search -- 3.2.1.1 Database Selection -- 3.2.1.2 Enzyme Specificity and Missed Cleavages -- 3.2.1.3 Posttranslational Modifications -- 3.2.1.4 Precursor Mass Tolerance -- 3.2.2 Target-Decoy Search Strategy for False Discovery Rate Estimation -- 3.2.3 Database Search Engines -- 3.2.3.1 SEQUEST -- 3.2.3.2 Mascot -- 3.2.3.3 Multiple Search Engines -- 3.2.4 Protein Inference -- 3.3 Relative Protein Quantification -- 3.3.1 Filtering -- 3.3.2 Missing Value Imputation -- 3.3.2.1 Imputation Methods -- 3.3.2.2 Comparative Studies -- 3.3.2.3 Normalization -- 3.3.3 Summarization -- 3.3.4 Differential Expression Analysis -- 3.4 Conclusions -- References -- Chapter 4 Data Processing in Metabolomics Capillary Electrophoresis-Mass Spectrometry -- 4.1 Data Extraction and the Interpretation of the Extracted Data -- 4.2 Data Preprocessing -- 4.2.1 Handling Missing Values -- 4.2.2 Normalization -- 4.2.3 Transformation -- 4.2.4 Scaling -- 4.3 Statistical Analysis -- 4.3.1 Two‐Sample T‐Test -- 4.3.2 Principal Component Analysis (PCA) -- 4.3.3 Partial Least‐Squares Discriminant Analysis (PLS-DA) -- 4.3.4 Support Vector Machine -- 4.3.5 Logistic Regression -- 4.3.6 Random Forest Model -- 4.3.7 Evaluation of Classification Models -- 4.4 Metabolite Identification -- References -- Chapter 5 Utility and Advances of Capillary Electrophoresis-Mass Spectrometry for Metabolomics -- 5.1 Introduction -- 5.2 Technological Developments -- 5.2.1 Improving Sensitivity -- 5.2.2 Increasing Metabolome Coverage -- 5.2.3 Increasing Annotation Capacity -- 5.2.4 Tackling Anionic Profiling -- 5.2.5 Increasing Sample Throughput -- 5.3 Applications -- 5.3.1 Biomedical Samples -- 5.3.2 Microbial Extracts -- 5.3.3 Plants -- 5.4 Concluding Remarks -- Acknowledgment -- Conflict of Interest.
References -- Chapter 6 Comprehensive Lipid Profiling by Multisegment Injection-Nonaqueous Capillary Electrophoresis-Mass Spectrometry: Expanding Coverage Beyond Hydrophilic Metabolites -- 6.1 The Early Origins of Lipidomics -- 6.2 Major Instrumental Platforms in Lipidomics: The Role of Separation Science -- 6.3 NACE-MS: An Emerging Separation Platform for Lipidomics? -- 6.4 Multiplexed Separations for Fatty Acids by MSI-NACE-MS -- 6.5 Comprehensive Lipid Profiling Strategies by MSI-NACE-MS -- 6.6 Future Perspectives and Summary -- References -- Chapter 7 Strategies for Identification of Modified Amino Acids with CE-MS in Metabolomics -- 7.1 Introduction -- 7.1.1 Post‐Translational Modifications and Modified Amino Acids -- 7.1.2 Modified Amino Acids as Biomarkers of Pathologies -- 7.2 Methods for the Detection of Modified Amino Acids -- 7.3 Capillary Electrophoresis Coupled to Mass Spectrometry to Analyze Modified Amino Acids -- 7.3.1 Applications of CE-MS for Analysis of Modified Amino Acids -- 7.4 Recent Developments to Enhance and Facilitate the Annotation Process in CE-MS -- Acknowledgments -- References -- Chapter 8 CE-MS Approaches for Single‐Cell Metabolomics -- 8.1 Introduction -- 8.2 Techniques for Single‐Cell Metabolome Analysis -- 8.2.1 Highly Sensitive CE-MS Interfacing -- 8.2.2 Online Sample Preconcentration Techniques -- 8.2.3 Isolation of Single Cells -- 8.3 Application to Single‐Cell Metabolome Analysis -- 8.3.1 Metabolome Analysis of Large Single Cells with Highly Sensitive Interface -- 8.3.2 Single‐Cell Metabolome Analysis with Highly Sensitive Interface and OSP Method -- 8.3.3 Single‐Cell Metabolome Analysis by Online Sampling and Highly Sensitive Interface -- 8.4 Conclusions -- References -- Chapter 9 CE-MS Approaches for Peptidomics -- 9.1 Introduction -- 9.2 Sample Preparation -- 9.3 CE-MS -- 9.3.1 Basic Characterization.
9.3.2 Peptide Identification -- 9.3.3 Peptide Quantitation -- 9.4 Applications -- 9.4.1 Search, Identification, and Determination of Biomarkers -- 9.4.2 Food Peptidomics -- 9.4.3 Other Applications -- 9.5 Conclusions -- Acknowledgments -- List of Abbreviations -- References -- Chapter 10 Capillary Zone Electrophoresis-Mass Spectrometry for Top‐Down Proteomics: Technological Development and Biological Applications -- 10.1 Introduction -- 10.2 Technological Development -- 10.2.1 CE-MS Interface -- 10.2.2 Capillary Coating -- 10.2.3 Sample Loading Capacity and Separation Window -- 10.2.4 Coupling Novel Gas‐Phase Fragmentation Techniques to CZE-MS/MS for TDP -- 10.2.5 Electrophoretic Mobility Prediction of Proteoforms -- 10.3 Applications of CZE-MS‐Based TDP -- 10.3.1 Delineation of Proteoforms of Complex Proteomes, Disease Biomarkers, and Biopharmaceuticals -- 10.3.2 CZE-MS for Native TDP -- 10.4 Conclusions and Perspectives -- Acknowledgments -- References -- Chapter 11 CE-MS Methods for the Characterization of Monoclonal Antibodies -- 11.1 Introduction -- 11.2 mAb Characterization Approaches -- 11.3 Applications -- 11.3.1 Primary Structure Characterization of Monoclonal Antibodies -- 11.3.1.1 Analytical Workflow -- 11.3.1.2 Amino Acid Sequence Characterization Using CE-MS(/MS) -- 11.3.1.3 PTMs Characterization and Relative Quantification Using CE-MS(/MS) -- 11.3.1.4 Glycosylation Determination Using CE-MS(/MS) -- 11.3.2 Middle‐Up/Middle‐Down Analysis -- 11.3.2.1 Analytical Workflow -- 11.3.2.2 mAb Analysis Using Middle‐Up Strategy -- 11.3.2.3 mAb Analysis Using Middle-Down Strategy -- 11.3.3 Intact Analysis -- 11.3.3.1 Analytical Workflow -- 11.3.3.2 mAb Analysis in Denaturing Conditions Using CE-MS -- 11.3.3.3 mAb Analysis in Native Conditions Using CE-MS -- 11.3.4 Automation: Future of CE-MS for mAb Analysis -- 11.4 Conclusion -- References.
Chapter 12 CE and CE-MS Approaches for Glycan Analysis -- 12.1 The Importance of Glycosylation -- 12.2 Capillary Electrophoresis in Analytical Glycomics -- 12.2.1 Sample Preparation for CE‐Based Glycan Analysis -- 12.2.2 Analysis of Oligosaccharides from Biological Samples -- 12.2.3 Structural Elucidation of Carbohydrates: The GU Value Approach -- 12.2.4 LIF Sensitivity and Quantification -- 12.3 CE-MS of Oligosaccharides -- 12.3.1 Major Parameters to Set the ESI for CE-MS of Derivatized Oligosaccharides -- 12.3.2 CE-MS for Quantitative Carbohydrate Analysis -- 12.3.3 CE-MS for Glycomic Studies -- 12.4 Conclusions and Future Prospective -- Acknowledgment -- References -- Chapter 13 CE-MS Approaches for Glyco(proteo)mic Analysis -- 13.1 Introduction -- 13.1.1 N‐Linked Glycosylation -- 13.1.2 O‐Linked Glycosylation -- 13.1.3 Glycosylation Workflows -- 13.1.4 Analytical Approaches -- 13.1.5 CE-MS Interfaces -- 13.2 Glycan Analysis by CE-MS -- 13.2.1 Glycan Derivatization Strategies -- 13.3 Glycopeptide Analysis -- 13.3.1 Sample Treatment -- 13.3.2 Separation Conditions -- 13.3.3 Applications -- 13.4 Protein Analysis -- 13.4.1 Sample Treatment -- 13.4.2 Separation Conditions -- 13.4.3 Applications -- 13.5 Conclusions and Outlook -- Acknowledgments -- List of Abbreviations -- References -- Index -- EULA.
Record Nr. UNINA-9910830168203321
Hoboken, New Jersey : , : John Wiley & Sons, , [2023]
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrophoresis and microchip capillary electrophoresis [[electronic resource] ] : principles, applications, and limitations / / edited by Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho
Capillary electrophoresis and microchip capillary electrophoresis [[electronic resource] ] : principles, applications, and limitations / / edited by Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho
Pubbl/distr/stampa Hoboken, N.J., : Wiley, 2013
Descrizione fisica xxii, 394 p. : ill
Disciplina 502.8/2
Altri autori (Persone) GarciaCarlos D. <1972->
Chumbimuni-TorresKarin Y
CarrilhoEmanuel <1965->
Soggetto topico Capillary electrophoresis
Microtechnique
ISBN 1-118-52998-7
1-118-53000-4
1-299-24217-0
1-118-52999-5
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910265226203321
Hoboken, N.J., : Wiley, 2013
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrophoresis and microchip capillary electrophoresis [[electronic resource] ] : principles, applications, and limitations / / edited by Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho
Capillary electrophoresis and microchip capillary electrophoresis [[electronic resource] ] : principles, applications, and limitations / / edited by Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho
Edizione [1st ed.]
Pubbl/distr/stampa Hoboken, N.J., : Wiley, 2013
Descrizione fisica xxii, 394 p. : ill
Disciplina 502.8/2
Altri autori (Persone) GarciaCarlos D. <1972->
Chumbimuni-TorresKarin Y
CarrilhoEmanuel <1965->
Soggetto topico Capillary electrophoresis
Microtechnique
ISBN 1-118-52998-7
1-118-53000-4
1-299-24217-0
1-118-52999-5
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Capillary Electrophoresis and Microchip Capillary Electrophoresis: Principles, Applications, and Limitations -- Contents -- Preface -- Acknowledgments -- Contributors -- 1 Critical Evaluation of the Use of Surfactants in Capillary Electrophoresis -- 1.1 Introduction -- 1.2 Surfactants for Wall Coatings -- 1.2.1 Controlling the Electroosmotic Flow -- 1.2.2 Preventing Adsorption to the Capillary -- 1.3 Surfactants as Buffer Additives -- 1.3.1 Micellar Electrokinetic Chromatography -- 1.3.2 Microemulsion Electrokinetic Chromatography -- 1.3.3 Nonaqueous Capillary Electrophoresis with Added Surfactants -- 1.4 Surfactants for Analyte Preconcentration -- 1.4.1 Sweeping -- 1.4.2 Transient Trapping -- 1.4.3 Analyte Focusing by Micelle Collapse -- 1.4.4 Micelle to Solvent Stacking -- 1.4.5 Combinations of Preconcentration Methods -- 1.4.6 Cloud Point Extraction -- 1.5 Surfactants and Detection in CE -- 1.5.1 Mass Spectrometry -- 1.5.2 Electrochemical Detection -- 1.6 Conclusions -- References -- 2 Sample Stacking: A Versatile Approach for Analyte Enrichment in CE and Microchip-CE -- 2.1 Introduction -- 2.2 Isotachophoresis -- 2.3 Chromatography-Based Sample Stacking -- 2.4 Methods Based on Electrophoretic Mobility and Velocity Manipulation (Electrophoretic Methods) -- 2.4.1 Field-Enhanced Sample Stacking (FESS) -- 2.4.2 Field-Enhanced Sample Injection (FESI) -- 2.4.3 Large-Volume Sample Stacking (LVSS) -- 2.4.4 Dynamic pH Junction -- 2.5 Sample Stacking in Pseudo-Stationary Phases -- 2.5.1 Field-Enhanced Sample Stacking -- 2.5.2 Hydrodynamic Injection Techniques -- 2.5.2.1 Normal Stacking Mode (NSM) -- 2.5.2.2 Reverse Electrode Polarity Stacking Mode (REPSM) -- 2.5.2.3 Stacking with Reverse Migrating Micelles (SRMM) -- 2.5.2.4 Stacking Using Reverse Migrating Micelles and a Water Plug (SRW) -- 2.5.2.5 High-Conductivity Sample Stacking (HCSS).
2.5.3 Electrokinetic Injection Techniques -- 2.5.3.1 Field-Enhanced Sample Injection (FESI-MEKC) -- 2.5.3.2 Field-Enhanced Sample Injection with Reverse Migrating Micelles (FESI-RMM) -- 2.5.4 Sweeping -- 2.5.5 Combined Techniques -- 2.5.5.1 Dynamic pH Junction: Sweeping -- 2.5.5.2 Selective Exhaustive Injection (SEI) -- 2.5.6 New Techniques -- 2.6 Stacking Techniques in Microchips -- 2.7 Concluding Remarks -- References -- 3 Sampling and Quantitative Analysis in Capillary Electrophoresis -- 3.1 Introduction -- 3.2 Injection Techniques in CE -- 3.2.1 Hydrodynamic Sample Injection -- 3.2.1.1 Principle -- 3.2.1.2 Advantages and Performance -- 3.2.1.3 Disadvantages -- 3.2.2 Electrokinetic Sample Injection -- 3.2.2.1 Principle -- 3.2.2.2 Advantages and Performance -- 3.2.2.3 Disadvantages -- 3.2.3 Bias-Free Electrokinetic Injection -- 3.2.4 Extraneous Sample Introduction Accompanying Injections in CE -- 3.2.5 Sample Stacking -- 3.2.5.1 Principle -- 3.2.5.2 Advantages and Performance -- 3.2.5.3 Disadvantages -- 3.2.6 Alternative Batch Sample Injection Techniques -- 3.2.6.1 Rotary-Type Injectors for CE -- 3.2.6.2 Hydrodynamic Sample Splitting as Injection Method for CE -- 3.2.6.3 Electrokinetic Sample Splitting as Injection Method for CE -- 3.2.6.4 Dual-Opposite End Injection in CE -- 3.3 Micromachined/Microchip Injection Devices -- 3.3.1 Droplet Sampler Based on Digital Microfluidics -- 3.3.2 Wire Loop Injection -- 3.4 Automated Flow Sample Injection and Hyphenated Systems -- 3.4.1 Introduction -- 3.4.2 Advantages and Performance -- 3.4.3 Disadvantages -- 3.5 Computerized Sampling and Data Analysis -- 3.6 Sampling in Portable CE Instrumentation -- 3.7 Quantitative Analysis in CE -- 3.7.1 Introduction -- 3.7.2 Quantitative Analysis with HD Injection -- 3.7.3 Quantitative Analysis with EK Injection -- 3.7.4 Validation of the Developed CE Methods.
3.7.5 Computer Data Treatment in Quantitative Analysis -- 3.8 Conclusions -- References -- 4 Practical Considerations for the Design and Implementation of High-Voltage Power Supplies for Capillary and Microchip Capillary Electrophoresis -- 4.1 Introduction -- 4.1.1 High-Voltage Fundamentals -- 4.1.2 Electroosmotic Flow Control -- 4.1.3 Technical Aspects -- 4.1.4 Construction of Bipolar HVPS from Unipolar HVPS -- 4.1.5 Safety Considerations -- 4.1.6 HVPS Commercially Available -- 4.1.7 Practical Considerations -- 4.1.8 Alternative Sources of HV -- 4.1.9 HVPS Controllers for MCE -- 4.2 High-Voltage Measurement -- 4.3 Concluding Remarks -- References -- 5 Artificial Neural Networks in Capillary Electrophoresis -- 5.1 Introduction -- 5.2 Optimization in CE: From Single Variable Approach Toward Artificial Neural Networks -- 5.2.1 Limitations of "Traditional" Single Variable Approach -- 5.2.2 Multivariate Approach with Experimental Design and Response Surface Modeling -- 5.2.2.1 Experimental Design -- 5.2.2.2 Response Surface Modeling -- 5.3 Artificial Neural Networks in Electromigration Methods -- 5.3.1 Introduction-Basic Principles of ANN -- 5.3.2 Optimization Using a Combination of ED and ANN -- 5.3.2.1 Testing of ED-ANN Algorithm -- 5.3.2.2 Practical Applications of ED-ANN -- 5.3.3 Quantitative CE Analysis and Determination from Overlapped Peaks -- 5.3.3.1 Evaluation of Calibration Plots in CE Using ANN to Increase Precision of Analysis -- 5.3.3.2 ANN in Quantitative CE Analysis from Overlapped Peaks -- 5.3.4 ANN in CEC and MEKC -- 5.3.5 ANN for Peptides Modeling -- 5.3.6 Classification and Fingerprinting -- 5.3.7 Other Applications -- 5.4 Conclusions -- Acknowledgments -- References -- 6 Improving the Separation in Microchip Electrophoresis by Surface Modification -- 6.1 Introduction -- 6.2 Strategies for Improving Separation.
6.2.1 Selection of an Adequate Technique: ME -- 6.2.2 Microchannel Design -- 6.2.3 Selection of an Appropriate ME Material -- 6.2.4 Optimization of the Working Conditions -- 6.2.5 Surface Modification -- 6.2.5.1 Surface Micro- and Nanostructuring -- 6.2.5.2 Employment of Energy Sources -- 6.2.5.3 Chemical Surface Modification -- 6.3 Chemical Modifiers -- 6.3.1 Surfactants -- 6.3.2 Ionic Liquids -- 6.3.3 Nanoparticles -- 6.3.4 Polymers -- 6.4 Conclusions -- Acknowledgments -- References -- 7 Capillary Electrophoretic Reactor and Microchip Capillary Electrophoretic Reactor: Dissociation Kinetic Analysis Method for "Complexes" Using Capillary Electrophoretic Separation Process -- 7.1 Introduction -- 7.2 Basic Concept of CER -- 7.3 Dissociation Kinetic Analysis of Metal Complexes Using a CER -- 7.3.1 Determination of the Rate Constants of Dissociation of 1:2 Complexes of Al3+ and Ga3+ with an Azo Dye Ligand 2,2'-Dihydroxyazobenzene-5,5'-Disulfonate in a CER -- 7.4 Expanding the Scope of the CER to Measurements of Fast Dissociation Kinetics with a Half-Life from Seconds to Dozens of Seconds: Dissociation Kinetic Analysis of Metal Complexes Using a Microchip Capillary Electrophoretic Reactor (µCER) -- 7.5 Expanding the Scope of the CER to the Measurement of Slow Dissociation Kinetics with a Half-Life of Hours -- 7.5.1 Principle of LS-CER -- 7.5.2 Application of LS-CER to the Ti(IV)-Catechin Complex -- 7.5.3 Application of LS-CER to the Ti(IV)-Tiron Complex -- 7.6 Expanding the Scope of CER to Measurement of the Dissociation Kinetics of Biomolecular Complexes -- 7.6.1 Dissociation Kinetic Analysis of [SSB-ssDNA] Using CER -- 7.7 Conclusions -- References -- 8 Capacitively Coupled Contactless Conductivity Detection (C4D) Applied to Capillary Electrophoresis (CE) and Microchip Electrophoresis (MCE) -- 8.1 Introduction -- 8.2 Theory of C4D.
8.2.1 Basic Principles of C4D -- 8.2.2 Simulation -- 8.2.3 Basic Equation for Sensitivity -- 8.2.4 Equivalent Circuit of a CE-C4D System -- 8.2.5 Practical Guidelines -- 8.3 C4D Applied to Capillary Electrophoresis -- 8.3.1 Instrumental Aspects in CE -- 8.3.2 Coupling C4D with UV-Vis Photometric Detectors in CE -- 8.3.3 Fundamental Studies in Capillary Electrophoresis Using C4D -- 8.3.4 Fundamental Studies on C4D -- 8.3.5 Applications -- 8.4 C4D Applied to Microchip Capillary Electrophoresis -- 8.4.1 Geometry of the Detection Electrodes -- 8.4.1.1 Embedded Electrodes -- 8.4.1.2 Attached Electrodes -- 8.4.1.3 External Electrodes -- 8.4.2 Applications -- 8.4.2.1 Bioanalytical Applications -- 8.4.2.2 On-Chip Enzymatic Reactions -- 8.4.2.3 Food Analysis -- 8.4.2.4 Explosives and Chemical Warfare Agents -- 8.4.2.5 Other Applications -- 8.5 Concluding Remarks -- Acknowledgments -- References -- 9 Capillary Electrophoresis with Electrochemical Detection -- 9.1 Principles of Electrochemical Detection -- 9.1.1 Amperometric Detection -- 9.1.2 Potentiometric Detection -- 9.1.3 Conductivity Detection -- 9.2 Interfacing Amperometric Detection to Capillary Electrophoresis -- 9.2.1 Off-Column Detection -- 9.2.2 End-Column Detection -- 9.2.3 Use of Multiple Detection Electrodes -- 9.2.4 Pulsed Amperometric Detection -- 9.2.5 Nonaqueous EC Detection -- 9.2.6 Electrode Material -- 9.2.7 Dual Conductivity and Amperometric Detection -- 9.3 Interfacing Electrochemical Detection to Microfluidic Capillary Electrophoresis -- 9.3.1 End-Column Detection -- 9.3.2 Pulsed Amperometric Detection -- 9.3.3 Off-Channel Detection -- 9.3.4 Electrode Material -- 9.3.5 Portable CE and MCE Systems -- 9.3.6 Applications of CE-MCE with AD -- 9.3.7 Future Directions for CE-MCE with EC Detection -- References.
10 Overcoming Challenges in Using Microchip Electrophoresis for Extended Monitoring Applications.
Record Nr. UNINA-9910812130103321
Hoboken, N.J., : Wiley, 2013
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Capillary electrophoresis for food analysis [[electronic resource] ] : method development / / Richard A. Frazier, Jennifer M. Ames and Harry E. Nursten
Capillary electrophoresis for food analysis [[electronic resource] ] : method development / / Richard A. Frazier, Jennifer M. Ames and Harry E. Nursten
Autore Frazier Richard A
Pubbl/distr/stampa Cambridge, : Royal Society of Chemistry, 2000
Descrizione fisica 1 online resource (143 p.)
Disciplina 664.07
Altri autori (Persone) AmesJennifer M
NurstenHarry E
Soggetto topico Capillary electrophoresis
Food - Analysis
Food adulteration and inspection
Soggetto genere / forma Electronic books.
ISBN 1-84755-031-2
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto OFC; BK9780854044924-FP001; BK9780854044924-FP005; BK9780854044924-FP007; BK9780854044924-FP011; BK9780854044924-00001; BK9780854044924-00008; BK9780854044924-00016; BK9780854044924-00025; BK9780854044924-00032; BK9780854044924-00047; BK9780854044924-00057; BK9780854044924-00061; BK9780854044924-00067; BK9780854044924-00088; BK9780854044924-00089; BK9780854044924-00095; BK9780854044924-00104; BK9780854044924-00113; BK9780854044924-00118
Record Nr. UNINA-9910455246603321
Frazier Richard A  
Cambridge, : Royal Society of Chemistry, 2000
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrophoresis for food analysis [[electronic resource] ] : method development / / Richard A. Frazier, Jennifer M. Ames and Harry E. Nursten
Capillary electrophoresis for food analysis [[electronic resource] ] : method development / / Richard A. Frazier, Jennifer M. Ames and Harry E. Nursten
Autore Frazier Richard A
Pubbl/distr/stampa Cambridge, : Royal Society of Chemistry, 2000
Descrizione fisica 1 online resource (143 p.)
Disciplina 664.07
Altri autori (Persone) AmesJennifer M
NurstenHarry E
Soggetto topico Capillary electrophoresis
Food - Analysis
Food adulteration and inspection
ISBN 1-84755-031-2
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto OFC; BK9780854044924-FP001; BK9780854044924-FP005; BK9780854044924-FP007; BK9780854044924-FP011; BK9780854044924-00001; BK9780854044924-00008; BK9780854044924-00016; BK9780854044924-00025; BK9780854044924-00032; BK9780854044924-00047; BK9780854044924-00057; BK9780854044924-00061; BK9780854044924-00067; BK9780854044924-00088; BK9780854044924-00089; BK9780854044924-00095; BK9780854044924-00104; BK9780854044924-00113; BK9780854044924-00118
Record Nr. UNINA-9910778311403321
Frazier Richard A  
Cambridge, : Royal Society of Chemistry, 2000
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui