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Biblioteca

MARC Lista (tabellare)
Capillary electrochromatography [[electronic resource] /] / edited by Keith D. Bartle, Peter Myers
Capillary electrochromatography [[electronic resource] /] / edited by Keith D. Bartle, Peter Myers
Pubbl/distr/stampa Cambridge, : Royal Society of Chemistry, 2001
Descrizione fisica 1 online resource (165 p.)
Disciplina 543.0894
Altri autori (Persone) BartleKeith D
MyersPeter
Collana RSC chromatography monographs
Soggetto topico Capillary electrophoresis
Capillary liquid chromatography
Soggetto genere / forma Electronic books.
ISBN 1-84755-030-4
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto BK9780854045303-FX001; 85ba00; 85ba00a; 85ba00b; 85ba00c; 85ba01; 85ba02; 85ba03; 85ba04; 85ba05; 85ba06; 85ba07; 85ba08; 85ba-ind
Record Nr. UNINA-9910455251403321
Cambridge, : Royal Society of Chemistry, 2001
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrochromatography [[electronic resource] /] / edited by Keith D. Bartle, Peter Myers
Capillary electrochromatography [[electronic resource] /] / edited by Keith D. Bartle, Peter Myers
Pubbl/distr/stampa Cambridge, : Royal Society of Chemistry, 2001
Descrizione fisica 1 online resource (165 p.)
Disciplina 543.0894
Altri autori (Persone) BartleKeith D
MyersPeter
Collana RSC chromatography monographs
Soggetto topico Capillary electrophoresis
Capillary liquid chromatography
ISBN 1-84755-030-4
Classificazione JE 92
VG 7500
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto BK9780854045303-FX001; 85ba00; 85ba00a; 85ba00b; 85ba00c; 85ba01; 85ba02; 85ba03; 85ba04; 85ba05; 85ba06; 85ba07; 85ba08; 85ba-ind
Record Nr. UNINA-9910778306003321
Cambridge, : Royal Society of Chemistry, 2001
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrophoresis - mass spectrometry (CE-MS) : principles and applications / / edited by Gerhardus de Jong
Capillary electrophoresis - mass spectrometry (CE-MS) : principles and applications / / edited by Gerhardus de Jong
Pubbl/distr/stampa Weinheim, Germany : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2016
Descrizione fisica 1 online resource (421 p.)
Disciplina 543.0871
Soggetto topico Capillary electrophoresis
Mass spectrometry
ISBN 3-527-69383-1
3-527-69381-5
3-527-69380-7
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910134854103321
Weinheim, Germany : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2016
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrophoresis -- mass spectrometry for proteomics and metabolomics : principles and applications / / edited by Rawi Ramautar and David D. Y. Chen
Capillary electrophoresis -- mass spectrometry for proteomics and metabolomics : principles and applications / / edited by Rawi Ramautar and David D. Y. Chen
Pubbl/distr/stampa Hoboken, New Jersey : , : John Wiley & Sons, , [2023]
Descrizione fisica 1 online resource (403 pages)
Disciplina 541.372
Soggetto topico Capillary electrophoresis
ISBN 3-527-83309-9
3-527-83308-0
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover -- Title Page -- Copyright -- Contents -- Preface -- Chapter 1 Capillary Electrophoresis-Mass Spectrometry Interfacing: Principles and Recent Developments -- 1.1 Introduction -- 1.2 General Considerations of CE-ESI-MS -- 1.2.1 Electrospray Ionization -- 1.2.2 Electrical Circuit in CE-ESI-MS -- 1.2.3 CE Modes and Conditions in CE-MS -- 1.3 Sheath Liquid Interfaces -- 1.3.1 Coaxial Sheath‐Flow ESI-MS Interface -- 1.3.2 Nanoflow Sheath Liquid ESI-MS Interface -- 1.4 Sheathless Interfaces -- 1.4.1 Porous‐Tip Interface -- 1.4.2 Other Sheathless Interfaces -- 1.5 Other CE-ESI-MS Interfaces -- 1.5.1 Liquid Junction -- 1.5.2 Interface‐Free CE-MS -- 1.6 Microchip Electrophoresis-MS Interfaces -- 1.7 Alternative Ionization Techniques for CE-MS -- 1.7.1 CE and MCE Combined with MALDI-MS -- 1.7.2 CE-ICP-MS -- 1.8 Concluding Remarks and Outlook -- List of Abbreviations -- References -- Chapter 2 Data Analysis Strategies in CE-MS for Metabolomics -- 2.1 Introduction -- 2.2 The Annotation Challenge in CE-MS‐Based Untargeted Metabolomics -- 2.2.1 Peak Picking in CE-MS Metabolomics -- 2.2.2 Alignment Approaches -- 2.2.3 μeff Transformation -- 2.2.3.1 Historical Evolution and Current State -- 2.2.3.2 Quantitative Aspects of the Use of Mobilograms -- 2.2.3.3 Technical Considerations of the μeff Transformation -- 2.2.4 Reproducibility and Exchange of μeff Information Using Libraries -- 2.2.5 Interlaboratory Reproducibility -- 2.2.6 ROMANCE -- 2.2.7 Ion Mobility -- 2.3 Data Pretreatment -- 2.3.1 Area Normalization -- 2.3.2 Analytical Quality Monitoring -- 2.3.3 Data Filtering -- 2.4 Data Treatment -- 2.5 Concluding Remarks -- Acknowledgment -- References -- Chapter 3 Data‐Processing Workflow for Relative Quantification from Label‐Free and Isobaric Labeling‐Based Untargeted Shotgun Proteomics: From Database Search to Differential Expression Analysis.
3.1 Introduction -- 3.2 Spectra Acquisition and Database Search -- 3.2.1 Parameters Affecting Sequence Database Search -- 3.2.1.1 Database Selection -- 3.2.1.2 Enzyme Specificity and Missed Cleavages -- 3.2.1.3 Posttranslational Modifications -- 3.2.1.4 Precursor Mass Tolerance -- 3.2.2 Target-Decoy Search Strategy for False Discovery Rate Estimation -- 3.2.3 Database Search Engines -- 3.2.3.1 SEQUEST -- 3.2.3.2 Mascot -- 3.2.3.3 Multiple Search Engines -- 3.2.4 Protein Inference -- 3.3 Relative Protein Quantification -- 3.3.1 Filtering -- 3.3.2 Missing Value Imputation -- 3.3.2.1 Imputation Methods -- 3.3.2.2 Comparative Studies -- 3.3.2.3 Normalization -- 3.3.3 Summarization -- 3.3.4 Differential Expression Analysis -- 3.4 Conclusions -- References -- Chapter 4 Data Processing in Metabolomics Capillary Electrophoresis-Mass Spectrometry -- 4.1 Data Extraction and the Interpretation of the Extracted Data -- 4.2 Data Preprocessing -- 4.2.1 Handling Missing Values -- 4.2.2 Normalization -- 4.2.3 Transformation -- 4.2.4 Scaling -- 4.3 Statistical Analysis -- 4.3.1 Two‐Sample T‐Test -- 4.3.2 Principal Component Analysis (PCA) -- 4.3.3 Partial Least‐Squares Discriminant Analysis (PLS-DA) -- 4.3.4 Support Vector Machine -- 4.3.5 Logistic Regression -- 4.3.6 Random Forest Model -- 4.3.7 Evaluation of Classification Models -- 4.4 Metabolite Identification -- References -- Chapter 5 Utility and Advances of Capillary Electrophoresis-Mass Spectrometry for Metabolomics -- 5.1 Introduction -- 5.2 Technological Developments -- 5.2.1 Improving Sensitivity -- 5.2.2 Increasing Metabolome Coverage -- 5.2.3 Increasing Annotation Capacity -- 5.2.4 Tackling Anionic Profiling -- 5.2.5 Increasing Sample Throughput -- 5.3 Applications -- 5.3.1 Biomedical Samples -- 5.3.2 Microbial Extracts -- 5.3.3 Plants -- 5.4 Concluding Remarks -- Acknowledgment -- Conflict of Interest.
References -- Chapter 6 Comprehensive Lipid Profiling by Multisegment Injection-Nonaqueous Capillary Electrophoresis-Mass Spectrometry: Expanding Coverage Beyond Hydrophilic Metabolites -- 6.1 The Early Origins of Lipidomics -- 6.2 Major Instrumental Platforms in Lipidomics: The Role of Separation Science -- 6.3 NACE-MS: An Emerging Separation Platform for Lipidomics? -- 6.4 Multiplexed Separations for Fatty Acids by MSI-NACE-MS -- 6.5 Comprehensive Lipid Profiling Strategies by MSI-NACE-MS -- 6.6 Future Perspectives and Summary -- References -- Chapter 7 Strategies for Identification of Modified Amino Acids with CE-MS in Metabolomics -- 7.1 Introduction -- 7.1.1 Post‐Translational Modifications and Modified Amino Acids -- 7.1.2 Modified Amino Acids as Biomarkers of Pathologies -- 7.2 Methods for the Detection of Modified Amino Acids -- 7.3 Capillary Electrophoresis Coupled to Mass Spectrometry to Analyze Modified Amino Acids -- 7.3.1 Applications of CE-MS for Analysis of Modified Amino Acids -- 7.4 Recent Developments to Enhance and Facilitate the Annotation Process in CE-MS -- Acknowledgments -- References -- Chapter 8 CE-MS Approaches for Single‐Cell Metabolomics -- 8.1 Introduction -- 8.2 Techniques for Single‐Cell Metabolome Analysis -- 8.2.1 Highly Sensitive CE-MS Interfacing -- 8.2.2 Online Sample Preconcentration Techniques -- 8.2.3 Isolation of Single Cells -- 8.3 Application to Single‐Cell Metabolome Analysis -- 8.3.1 Metabolome Analysis of Large Single Cells with Highly Sensitive Interface -- 8.3.2 Single‐Cell Metabolome Analysis with Highly Sensitive Interface and OSP Method -- 8.3.3 Single‐Cell Metabolome Analysis by Online Sampling and Highly Sensitive Interface -- 8.4 Conclusions -- References -- Chapter 9 CE-MS Approaches for Peptidomics -- 9.1 Introduction -- 9.2 Sample Preparation -- 9.3 CE-MS -- 9.3.1 Basic Characterization.
9.3.2 Peptide Identification -- 9.3.3 Peptide Quantitation -- 9.4 Applications -- 9.4.1 Search, Identification, and Determination of Biomarkers -- 9.4.2 Food Peptidomics -- 9.4.3 Other Applications -- 9.5 Conclusions -- Acknowledgments -- List of Abbreviations -- References -- Chapter 10 Capillary Zone Electrophoresis-Mass Spectrometry for Top‐Down Proteomics: Technological Development and Biological Applications -- 10.1 Introduction -- 10.2 Technological Development -- 10.2.1 CE-MS Interface -- 10.2.2 Capillary Coating -- 10.2.3 Sample Loading Capacity and Separation Window -- 10.2.4 Coupling Novel Gas‐Phase Fragmentation Techniques to CZE-MS/MS for TDP -- 10.2.5 Electrophoretic Mobility Prediction of Proteoforms -- 10.3 Applications of CZE-MS‐Based TDP -- 10.3.1 Delineation of Proteoforms of Complex Proteomes, Disease Biomarkers, and Biopharmaceuticals -- 10.3.2 CZE-MS for Native TDP -- 10.4 Conclusions and Perspectives -- Acknowledgments -- References -- Chapter 11 CE-MS Methods for the Characterization of Monoclonal Antibodies -- 11.1 Introduction -- 11.2 mAb Characterization Approaches -- 11.3 Applications -- 11.3.1 Primary Structure Characterization of Monoclonal Antibodies -- 11.3.1.1 Analytical Workflow -- 11.3.1.2 Amino Acid Sequence Characterization Using CE-MS(/MS) -- 11.3.1.3 PTMs Characterization and Relative Quantification Using CE-MS(/MS) -- 11.3.1.4 Glycosylation Determination Using CE-MS(/MS) -- 11.3.2 Middle‐Up/Middle‐Down Analysis -- 11.3.2.1 Analytical Workflow -- 11.3.2.2 mAb Analysis Using Middle‐Up Strategy -- 11.3.2.3 mAb Analysis Using Middle-Down Strategy -- 11.3.3 Intact Analysis -- 11.3.3.1 Analytical Workflow -- 11.3.3.2 mAb Analysis in Denaturing Conditions Using CE-MS -- 11.3.3.3 mAb Analysis in Native Conditions Using CE-MS -- 11.3.4 Automation: Future of CE-MS for mAb Analysis -- 11.4 Conclusion -- References.
Chapter 12 CE and CE-MS Approaches for Glycan Analysis -- 12.1 The Importance of Glycosylation -- 12.2 Capillary Electrophoresis in Analytical Glycomics -- 12.2.1 Sample Preparation for CE‐Based Glycan Analysis -- 12.2.2 Analysis of Oligosaccharides from Biological Samples -- 12.2.3 Structural Elucidation of Carbohydrates: The GU Value Approach -- 12.2.4 LIF Sensitivity and Quantification -- 12.3 CE-MS of Oligosaccharides -- 12.3.1 Major Parameters to Set the ESI for CE-MS of Derivatized Oligosaccharides -- 12.3.2 CE-MS for Quantitative Carbohydrate Analysis -- 12.3.3 CE-MS for Glycomic Studies -- 12.4 Conclusions and Future Prospective -- Acknowledgment -- References -- Chapter 13 CE-MS Approaches for Glyco(proteo)mic Analysis -- 13.1 Introduction -- 13.1.1 N‐Linked Glycosylation -- 13.1.2 O‐Linked Glycosylation -- 13.1.3 Glycosylation Workflows -- 13.1.4 Analytical Approaches -- 13.1.5 CE-MS Interfaces -- 13.2 Glycan Analysis by CE-MS -- 13.2.1 Glycan Derivatization Strategies -- 13.3 Glycopeptide Analysis -- 13.3.1 Sample Treatment -- 13.3.2 Separation Conditions -- 13.3.3 Applications -- 13.4 Protein Analysis -- 13.4.1 Sample Treatment -- 13.4.2 Separation Conditions -- 13.4.3 Applications -- 13.5 Conclusions and Outlook -- Acknowledgments -- List of Abbreviations -- References -- Index -- EULA.
Record Nr. UNINA-9910644087903321
Hoboken, New Jersey : , : John Wiley & Sons, , [2023]
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrophoresis -- mass spectrometry for proteomics and metabolomics : principles and applications / / edited by Rawi Ramautar and David D. Y. Chen
Capillary electrophoresis -- mass spectrometry for proteomics and metabolomics : principles and applications / / edited by Rawi Ramautar and David D. Y. Chen
Pubbl/distr/stampa Hoboken, New Jersey : , : John Wiley & Sons, , [2023]
Descrizione fisica 1 online resource (403 pages)
Disciplina 541.372
Soggetto topico Capillary electrophoresis
ISBN 3-527-83309-9
3-527-83308-0
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover -- Title Page -- Copyright -- Contents -- Preface -- Chapter 1 Capillary Electrophoresis-Mass Spectrometry Interfacing: Principles and Recent Developments -- 1.1 Introduction -- 1.2 General Considerations of CE-ESI-MS -- 1.2.1 Electrospray Ionization -- 1.2.2 Electrical Circuit in CE-ESI-MS -- 1.2.3 CE Modes and Conditions in CE-MS -- 1.3 Sheath Liquid Interfaces -- 1.3.1 Coaxial Sheath‐Flow ESI-MS Interface -- 1.3.2 Nanoflow Sheath Liquid ESI-MS Interface -- 1.4 Sheathless Interfaces -- 1.4.1 Porous‐Tip Interface -- 1.4.2 Other Sheathless Interfaces -- 1.5 Other CE-ESI-MS Interfaces -- 1.5.1 Liquid Junction -- 1.5.2 Interface‐Free CE-MS -- 1.6 Microchip Electrophoresis-MS Interfaces -- 1.7 Alternative Ionization Techniques for CE-MS -- 1.7.1 CE and MCE Combined with MALDI-MS -- 1.7.2 CE-ICP-MS -- 1.8 Concluding Remarks and Outlook -- List of Abbreviations -- References -- Chapter 2 Data Analysis Strategies in CE-MS for Metabolomics -- 2.1 Introduction -- 2.2 The Annotation Challenge in CE-MS‐Based Untargeted Metabolomics -- 2.2.1 Peak Picking in CE-MS Metabolomics -- 2.2.2 Alignment Approaches -- 2.2.3 μeff Transformation -- 2.2.3.1 Historical Evolution and Current State -- 2.2.3.2 Quantitative Aspects of the Use of Mobilograms -- 2.2.3.3 Technical Considerations of the μeff Transformation -- 2.2.4 Reproducibility and Exchange of μeff Information Using Libraries -- 2.2.5 Interlaboratory Reproducibility -- 2.2.6 ROMANCE -- 2.2.7 Ion Mobility -- 2.3 Data Pretreatment -- 2.3.1 Area Normalization -- 2.3.2 Analytical Quality Monitoring -- 2.3.3 Data Filtering -- 2.4 Data Treatment -- 2.5 Concluding Remarks -- Acknowledgment -- References -- Chapter 3 Data‐Processing Workflow for Relative Quantification from Label‐Free and Isobaric Labeling‐Based Untargeted Shotgun Proteomics: From Database Search to Differential Expression Analysis.
3.1 Introduction -- 3.2 Spectra Acquisition and Database Search -- 3.2.1 Parameters Affecting Sequence Database Search -- 3.2.1.1 Database Selection -- 3.2.1.2 Enzyme Specificity and Missed Cleavages -- 3.2.1.3 Posttranslational Modifications -- 3.2.1.4 Precursor Mass Tolerance -- 3.2.2 Target-Decoy Search Strategy for False Discovery Rate Estimation -- 3.2.3 Database Search Engines -- 3.2.3.1 SEQUEST -- 3.2.3.2 Mascot -- 3.2.3.3 Multiple Search Engines -- 3.2.4 Protein Inference -- 3.3 Relative Protein Quantification -- 3.3.1 Filtering -- 3.3.2 Missing Value Imputation -- 3.3.2.1 Imputation Methods -- 3.3.2.2 Comparative Studies -- 3.3.2.3 Normalization -- 3.3.3 Summarization -- 3.3.4 Differential Expression Analysis -- 3.4 Conclusions -- References -- Chapter 4 Data Processing in Metabolomics Capillary Electrophoresis-Mass Spectrometry -- 4.1 Data Extraction and the Interpretation of the Extracted Data -- 4.2 Data Preprocessing -- 4.2.1 Handling Missing Values -- 4.2.2 Normalization -- 4.2.3 Transformation -- 4.2.4 Scaling -- 4.3 Statistical Analysis -- 4.3.1 Two‐Sample T‐Test -- 4.3.2 Principal Component Analysis (PCA) -- 4.3.3 Partial Least‐Squares Discriminant Analysis (PLS-DA) -- 4.3.4 Support Vector Machine -- 4.3.5 Logistic Regression -- 4.3.6 Random Forest Model -- 4.3.7 Evaluation of Classification Models -- 4.4 Metabolite Identification -- References -- Chapter 5 Utility and Advances of Capillary Electrophoresis-Mass Spectrometry for Metabolomics -- 5.1 Introduction -- 5.2 Technological Developments -- 5.2.1 Improving Sensitivity -- 5.2.2 Increasing Metabolome Coverage -- 5.2.3 Increasing Annotation Capacity -- 5.2.4 Tackling Anionic Profiling -- 5.2.5 Increasing Sample Throughput -- 5.3 Applications -- 5.3.1 Biomedical Samples -- 5.3.2 Microbial Extracts -- 5.3.3 Plants -- 5.4 Concluding Remarks -- Acknowledgment -- Conflict of Interest.
References -- Chapter 6 Comprehensive Lipid Profiling by Multisegment Injection-Nonaqueous Capillary Electrophoresis-Mass Spectrometry: Expanding Coverage Beyond Hydrophilic Metabolites -- 6.1 The Early Origins of Lipidomics -- 6.2 Major Instrumental Platforms in Lipidomics: The Role of Separation Science -- 6.3 NACE-MS: An Emerging Separation Platform for Lipidomics? -- 6.4 Multiplexed Separations for Fatty Acids by MSI-NACE-MS -- 6.5 Comprehensive Lipid Profiling Strategies by MSI-NACE-MS -- 6.6 Future Perspectives and Summary -- References -- Chapter 7 Strategies for Identification of Modified Amino Acids with CE-MS in Metabolomics -- 7.1 Introduction -- 7.1.1 Post‐Translational Modifications and Modified Amino Acids -- 7.1.2 Modified Amino Acids as Biomarkers of Pathologies -- 7.2 Methods for the Detection of Modified Amino Acids -- 7.3 Capillary Electrophoresis Coupled to Mass Spectrometry to Analyze Modified Amino Acids -- 7.3.1 Applications of CE-MS for Analysis of Modified Amino Acids -- 7.4 Recent Developments to Enhance and Facilitate the Annotation Process in CE-MS -- Acknowledgments -- References -- Chapter 8 CE-MS Approaches for Single‐Cell Metabolomics -- 8.1 Introduction -- 8.2 Techniques for Single‐Cell Metabolome Analysis -- 8.2.1 Highly Sensitive CE-MS Interfacing -- 8.2.2 Online Sample Preconcentration Techniques -- 8.2.3 Isolation of Single Cells -- 8.3 Application to Single‐Cell Metabolome Analysis -- 8.3.1 Metabolome Analysis of Large Single Cells with Highly Sensitive Interface -- 8.3.2 Single‐Cell Metabolome Analysis with Highly Sensitive Interface and OSP Method -- 8.3.3 Single‐Cell Metabolome Analysis by Online Sampling and Highly Sensitive Interface -- 8.4 Conclusions -- References -- Chapter 9 CE-MS Approaches for Peptidomics -- 9.1 Introduction -- 9.2 Sample Preparation -- 9.3 CE-MS -- 9.3.1 Basic Characterization.
9.3.2 Peptide Identification -- 9.3.3 Peptide Quantitation -- 9.4 Applications -- 9.4.1 Search, Identification, and Determination of Biomarkers -- 9.4.2 Food Peptidomics -- 9.4.3 Other Applications -- 9.5 Conclusions -- Acknowledgments -- List of Abbreviations -- References -- Chapter 10 Capillary Zone Electrophoresis-Mass Spectrometry for Top‐Down Proteomics: Technological Development and Biological Applications -- 10.1 Introduction -- 10.2 Technological Development -- 10.2.1 CE-MS Interface -- 10.2.2 Capillary Coating -- 10.2.3 Sample Loading Capacity and Separation Window -- 10.2.4 Coupling Novel Gas‐Phase Fragmentation Techniques to CZE-MS/MS for TDP -- 10.2.5 Electrophoretic Mobility Prediction of Proteoforms -- 10.3 Applications of CZE-MS‐Based TDP -- 10.3.1 Delineation of Proteoforms of Complex Proteomes, Disease Biomarkers, and Biopharmaceuticals -- 10.3.2 CZE-MS for Native TDP -- 10.4 Conclusions and Perspectives -- Acknowledgments -- References -- Chapter 11 CE-MS Methods for the Characterization of Monoclonal Antibodies -- 11.1 Introduction -- 11.2 mAb Characterization Approaches -- 11.3 Applications -- 11.3.1 Primary Structure Characterization of Monoclonal Antibodies -- 11.3.1.1 Analytical Workflow -- 11.3.1.2 Amino Acid Sequence Characterization Using CE-MS(/MS) -- 11.3.1.3 PTMs Characterization and Relative Quantification Using CE-MS(/MS) -- 11.3.1.4 Glycosylation Determination Using CE-MS(/MS) -- 11.3.2 Middle‐Up/Middle‐Down Analysis -- 11.3.2.1 Analytical Workflow -- 11.3.2.2 mAb Analysis Using Middle‐Up Strategy -- 11.3.2.3 mAb Analysis Using Middle-Down Strategy -- 11.3.3 Intact Analysis -- 11.3.3.1 Analytical Workflow -- 11.3.3.2 mAb Analysis in Denaturing Conditions Using CE-MS -- 11.3.3.3 mAb Analysis in Native Conditions Using CE-MS -- 11.3.4 Automation: Future of CE-MS for mAb Analysis -- 11.4 Conclusion -- References.
Chapter 12 CE and CE-MS Approaches for Glycan Analysis -- 12.1 The Importance of Glycosylation -- 12.2 Capillary Electrophoresis in Analytical Glycomics -- 12.2.1 Sample Preparation for CE‐Based Glycan Analysis -- 12.2.2 Analysis of Oligosaccharides from Biological Samples -- 12.2.3 Structural Elucidation of Carbohydrates: The GU Value Approach -- 12.2.4 LIF Sensitivity and Quantification -- 12.3 CE-MS of Oligosaccharides -- 12.3.1 Major Parameters to Set the ESI for CE-MS of Derivatized Oligosaccharides -- 12.3.2 CE-MS for Quantitative Carbohydrate Analysis -- 12.3.3 CE-MS for Glycomic Studies -- 12.4 Conclusions and Future Prospective -- Acknowledgment -- References -- Chapter 13 CE-MS Approaches for Glyco(proteo)mic Analysis -- 13.1 Introduction -- 13.1.1 N‐Linked Glycosylation -- 13.1.2 O‐Linked Glycosylation -- 13.1.3 Glycosylation Workflows -- 13.1.4 Analytical Approaches -- 13.1.5 CE-MS Interfaces -- 13.2 Glycan Analysis by CE-MS -- 13.2.1 Glycan Derivatization Strategies -- 13.3 Glycopeptide Analysis -- 13.3.1 Sample Treatment -- 13.3.2 Separation Conditions -- 13.3.3 Applications -- 13.4 Protein Analysis -- 13.4.1 Sample Treatment -- 13.4.2 Separation Conditions -- 13.4.3 Applications -- 13.5 Conclusions and Outlook -- Acknowledgments -- List of Abbreviations -- References -- Index -- EULA.
Record Nr. UNINA-9910830168203321
Hoboken, New Jersey : , : John Wiley & Sons, , [2023]
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrophoresis and microchip capillary electrophoresis [[electronic resource] ] : principles, applications, and limitations / / edited by Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho
Capillary electrophoresis and microchip capillary electrophoresis [[electronic resource] ] : principles, applications, and limitations / / edited by Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho
Pubbl/distr/stampa Hoboken, N.J., : Wiley, 2013
Descrizione fisica xxii, 394 p. : ill
Disciplina 502.8/2
Altri autori (Persone) GarciaCarlos D. <1972->
Chumbimuni-TorresKarin Y
CarrilhoEmanuel <1965->
Soggetto topico Capillary electrophoresis
Microtechnique
ISBN 1-118-52998-7
1-118-53000-4
1-299-24217-0
1-118-52999-5
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910265226203321
Hoboken, N.J., : Wiley, 2013
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Capillary electrophoresis and microchip capillary electrophoresis : principles, applications, and limitations / / edited by Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho
Capillary electrophoresis and microchip capillary electrophoresis : principles, applications, and limitations / / edited by Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho
Edizione [1st ed.]
Pubbl/distr/stampa Hoboken, N.J., : Wiley, 2013
Descrizione fisica xxii, 394 p. : ill
Disciplina 502.8/2
Altri autori (Persone) GarciaCarlos D. <1972->
Chumbimuni-TorresKarin Y
CarrilhoEmanuel <1965->
Soggetto topico Capillary electrophoresis
Microtechnique
ISBN 9781118529980
1118529987
9781118530009
1118530004
9781299242173
1299242170
9781118529997
1118529995
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Capillary Electrophoresis and Microchip Capillary Electrophoresis: Principles, Applications, and Limitations -- Contents -- Preface -- Acknowledgments -- Contributors -- 1 Critical Evaluation of the Use of Surfactants in Capillary Electrophoresis -- 1.1 Introduction -- 1.2 Surfactants for Wall Coatings -- 1.2.1 Controlling the Electroosmotic Flow -- 1.2.2 Preventing Adsorption to the Capillary -- 1.3 Surfactants as Buffer Additives -- 1.3.1 Micellar Electrokinetic Chromatography -- 1.3.2 Microemulsion Electrokinetic Chromatography -- 1.3.3 Nonaqueous Capillary Electrophoresis with Added Surfactants -- 1.4 Surfactants for Analyte Preconcentration -- 1.4.1 Sweeping -- 1.4.2 Transient Trapping -- 1.4.3 Analyte Focusing by Micelle Collapse -- 1.4.4 Micelle to Solvent Stacking -- 1.4.5 Combinations of Preconcentration Methods -- 1.4.6 Cloud Point Extraction -- 1.5 Surfactants and Detection in CE -- 1.5.1 Mass Spectrometry -- 1.5.2 Electrochemical Detection -- 1.6 Conclusions -- References -- 2 Sample Stacking: A Versatile Approach for Analyte Enrichment in CE and Microchip-CE -- 2.1 Introduction -- 2.2 Isotachophoresis -- 2.3 Chromatography-Based Sample Stacking -- 2.4 Methods Based on Electrophoretic Mobility and Velocity Manipulation (Electrophoretic Methods) -- 2.4.1 Field-Enhanced Sample Stacking (FESS) -- 2.4.2 Field-Enhanced Sample Injection (FESI) -- 2.4.3 Large-Volume Sample Stacking (LVSS) -- 2.4.4 Dynamic pH Junction -- 2.5 Sample Stacking in Pseudo-Stationary Phases -- 2.5.1 Field-Enhanced Sample Stacking -- 2.5.2 Hydrodynamic Injection Techniques -- 2.5.2.1 Normal Stacking Mode (NSM) -- 2.5.2.2 Reverse Electrode Polarity Stacking Mode (REPSM) -- 2.5.2.3 Stacking with Reverse Migrating Micelles (SRMM) -- 2.5.2.4 Stacking Using Reverse Migrating Micelles and a Water Plug (SRW) -- 2.5.2.5 High-Conductivity Sample Stacking (HCSS).
2.5.3 Electrokinetic Injection Techniques -- 2.5.3.1 Field-Enhanced Sample Injection (FESI-MEKC) -- 2.5.3.2 Field-Enhanced Sample Injection with Reverse Migrating Micelles (FESI-RMM) -- 2.5.4 Sweeping -- 2.5.5 Combined Techniques -- 2.5.5.1 Dynamic pH Junction: Sweeping -- 2.5.5.2 Selective Exhaustive Injection (SEI) -- 2.5.6 New Techniques -- 2.6 Stacking Techniques in Microchips -- 2.7 Concluding Remarks -- References -- 3 Sampling and Quantitative Analysis in Capillary Electrophoresis -- 3.1 Introduction -- 3.2 Injection Techniques in CE -- 3.2.1 Hydrodynamic Sample Injection -- 3.2.1.1 Principle -- 3.2.1.2 Advantages and Performance -- 3.2.1.3 Disadvantages -- 3.2.2 Electrokinetic Sample Injection -- 3.2.2.1 Principle -- 3.2.2.2 Advantages and Performance -- 3.2.2.3 Disadvantages -- 3.2.3 Bias-Free Electrokinetic Injection -- 3.2.4 Extraneous Sample Introduction Accompanying Injections in CE -- 3.2.5 Sample Stacking -- 3.2.5.1 Principle -- 3.2.5.2 Advantages and Performance -- 3.2.5.3 Disadvantages -- 3.2.6 Alternative Batch Sample Injection Techniques -- 3.2.6.1 Rotary-Type Injectors for CE -- 3.2.6.2 Hydrodynamic Sample Splitting as Injection Method for CE -- 3.2.6.3 Electrokinetic Sample Splitting as Injection Method for CE -- 3.2.6.4 Dual-Opposite End Injection in CE -- 3.3 Micromachined/Microchip Injection Devices -- 3.3.1 Droplet Sampler Based on Digital Microfluidics -- 3.3.2 Wire Loop Injection -- 3.4 Automated Flow Sample Injection and Hyphenated Systems -- 3.4.1 Introduction -- 3.4.2 Advantages and Performance -- 3.4.3 Disadvantages -- 3.5 Computerized Sampling and Data Analysis -- 3.6 Sampling in Portable CE Instrumentation -- 3.7 Quantitative Analysis in CE -- 3.7.1 Introduction -- 3.7.2 Quantitative Analysis with HD Injection -- 3.7.3 Quantitative Analysis with EK Injection -- 3.7.4 Validation of the Developed CE Methods.
3.7.5 Computer Data Treatment in Quantitative Analysis -- 3.8 Conclusions -- References -- 4 Practical Considerations for the Design and Implementation of High-Voltage Power Supplies for Capillary and Microchip Capillary Electrophoresis -- 4.1 Introduction -- 4.1.1 High-Voltage Fundamentals -- 4.1.2 Electroosmotic Flow Control -- 4.1.3 Technical Aspects -- 4.1.4 Construction of Bipolar HVPS from Unipolar HVPS -- 4.1.5 Safety Considerations -- 4.1.6 HVPS Commercially Available -- 4.1.7 Practical Considerations -- 4.1.8 Alternative Sources of HV -- 4.1.9 HVPS Controllers for MCE -- 4.2 High-Voltage Measurement -- 4.3 Concluding Remarks -- References -- 5 Artificial Neural Networks in Capillary Electrophoresis -- 5.1 Introduction -- 5.2 Optimization in CE: From Single Variable Approach Toward Artificial Neural Networks -- 5.2.1 Limitations of "Traditional" Single Variable Approach -- 5.2.2 Multivariate Approach with Experimental Design and Response Surface Modeling -- 5.2.2.1 Experimental Design -- 5.2.2.2 Response Surface Modeling -- 5.3 Artificial Neural Networks in Electromigration Methods -- 5.3.1 Introduction-Basic Principles of ANN -- 5.3.2 Optimization Using a Combination of ED and ANN -- 5.3.2.1 Testing of ED-ANN Algorithm -- 5.3.2.2 Practical Applications of ED-ANN -- 5.3.3 Quantitative CE Analysis and Determination from Overlapped Peaks -- 5.3.3.1 Evaluation of Calibration Plots in CE Using ANN to Increase Precision of Analysis -- 5.3.3.2 ANN in Quantitative CE Analysis from Overlapped Peaks -- 5.3.4 ANN in CEC and MEKC -- 5.3.5 ANN for Peptides Modeling -- 5.3.6 Classification and Fingerprinting -- 5.3.7 Other Applications -- 5.4 Conclusions -- Acknowledgments -- References -- 6 Improving the Separation in Microchip Electrophoresis by Surface Modification -- 6.1 Introduction -- 6.2 Strategies for Improving Separation.
6.2.1 Selection of an Adequate Technique: ME -- 6.2.2 Microchannel Design -- 6.2.3 Selection of an Appropriate ME Material -- 6.2.4 Optimization of the Working Conditions -- 6.2.5 Surface Modification -- 6.2.5.1 Surface Micro- and Nanostructuring -- 6.2.5.2 Employment of Energy Sources -- 6.2.5.3 Chemical Surface Modification -- 6.3 Chemical Modifiers -- 6.3.1 Surfactants -- 6.3.2 Ionic Liquids -- 6.3.3 Nanoparticles -- 6.3.4 Polymers -- 6.4 Conclusions -- Acknowledgments -- References -- 7 Capillary Electrophoretic Reactor and Microchip Capillary Electrophoretic Reactor: Dissociation Kinetic Analysis Method for "Complexes" Using Capillary Electrophoretic Separation Process -- 7.1 Introduction -- 7.2 Basic Concept of CER -- 7.3 Dissociation Kinetic Analysis of Metal Complexes Using a CER -- 7.3.1 Determination of the Rate Constants of Dissociation of 1:2 Complexes of Al3+ and Ga3+ with an Azo Dye Ligand 2,2'-Dihydroxyazobenzene-5,5'-Disulfonate in a CER -- 7.4 Expanding the Scope of the CER to Measurements of Fast Dissociation Kinetics with a Half-Life from Seconds to Dozens of Seconds: Dissociation Kinetic Analysis of Metal Complexes Using a Microchip Capillary Electrophoretic Reactor (µCER) -- 7.5 Expanding the Scope of the CER to the Measurement of Slow Dissociation Kinetics with a Half-Life of Hours -- 7.5.1 Principle of LS-CER -- 7.5.2 Application of LS-CER to the Ti(IV)-Catechin Complex -- 7.5.3 Application of LS-CER to the Ti(IV)-Tiron Complex -- 7.6 Expanding the Scope of CER to Measurement of the Dissociation Kinetics of Biomolecular Complexes -- 7.6.1 Dissociation Kinetic Analysis of [SSB-ssDNA] Using CER -- 7.7 Conclusions -- References -- 8 Capacitively Coupled Contactless Conductivity Detection (C4D) Applied to Capillary Electrophoresis (CE) and Microchip Electrophoresis (MCE) -- 8.1 Introduction -- 8.2 Theory of C4D.
8.2.1 Basic Principles of C4D -- 8.2.2 Simulation -- 8.2.3 Basic Equation for Sensitivity -- 8.2.4 Equivalent Circuit of a CE-C4D System -- 8.2.5 Practical Guidelines -- 8.3 C4D Applied to Capillary Electrophoresis -- 8.3.1 Instrumental Aspects in CE -- 8.3.2 Coupling C4D with UV-Vis Photometric Detectors in CE -- 8.3.3 Fundamental Studies in Capillary Electrophoresis Using C4D -- 8.3.4 Fundamental Studies on C4D -- 8.3.5 Applications -- 8.4 C4D Applied to Microchip Capillary Electrophoresis -- 8.4.1 Geometry of the Detection Electrodes -- 8.4.1.1 Embedded Electrodes -- 8.4.1.2 Attached Electrodes -- 8.4.1.3 External Electrodes -- 8.4.2 Applications -- 8.4.2.1 Bioanalytical Applications -- 8.4.2.2 On-Chip Enzymatic Reactions -- 8.4.2.3 Food Analysis -- 8.4.2.4 Explosives and Chemical Warfare Agents -- 8.4.2.5 Other Applications -- 8.5 Concluding Remarks -- Acknowledgments -- References -- 9 Capillary Electrophoresis with Electrochemical Detection -- 9.1 Principles of Electrochemical Detection -- 9.1.1 Amperometric Detection -- 9.1.2 Potentiometric Detection -- 9.1.3 Conductivity Detection -- 9.2 Interfacing Amperometric Detection to Capillary Electrophoresis -- 9.2.1 Off-Column Detection -- 9.2.2 End-Column Detection -- 9.2.3 Use of Multiple Detection Electrodes -- 9.2.4 Pulsed Amperometric Detection -- 9.2.5 Nonaqueous EC Detection -- 9.2.6 Electrode Material -- 9.2.7 Dual Conductivity and Amperometric Detection -- 9.3 Interfacing Electrochemical Detection to Microfluidic Capillary Electrophoresis -- 9.3.1 End-Column Detection -- 9.3.2 Pulsed Amperometric Detection -- 9.3.3 Off-Channel Detection -- 9.3.4 Electrode Material -- 9.3.5 Portable CE and MCE Systems -- 9.3.6 Applications of CE-MCE with AD -- 9.3.7 Future Directions for CE-MCE with EC Detection -- References.
10 Overcoming Challenges in Using Microchip Electrophoresis for Extended Monitoring Applications.
Record Nr. UNINA-9910812130103321
Hoboken, N.J., : Wiley, 2013
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Capillary electrophoresis for food analysis [[electronic resource] ] : method development / / Richard A. Frazier, Jennifer M. Ames and Harry E. Nursten
Capillary electrophoresis for food analysis [[electronic resource] ] : method development / / Richard A. Frazier, Jennifer M. Ames and Harry E. Nursten
Autore Frazier Richard A
Pubbl/distr/stampa Cambridge, : Royal Society of Chemistry, 2000
Descrizione fisica 1 online resource (143 p.)
Disciplina 664.07
Altri autori (Persone) AmesJennifer M
NurstenHarry E
Soggetto topico Capillary electrophoresis
Food - Analysis
Food adulteration and inspection
Soggetto genere / forma Electronic books.
ISBN 1-84755-031-2
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto OFC; BK9780854044924-FP001; BK9780854044924-FP005; BK9780854044924-FP007; BK9780854044924-FP011; BK9780854044924-00001; BK9780854044924-00008; BK9780854044924-00016; BK9780854044924-00025; BK9780854044924-00032; BK9780854044924-00047; BK9780854044924-00057; BK9780854044924-00061; BK9780854044924-00067; BK9780854044924-00088; BK9780854044924-00089; BK9780854044924-00095; BK9780854044924-00104; BK9780854044924-00113; BK9780854044924-00118
Record Nr. UNINA-9910455246603321
Frazier Richard A  
Cambridge, : Royal Society of Chemistry, 2000
Materiale a stampa
Lo trovi qui: Univ. Federico II
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Capillary electrophoresis for food analysis [[electronic resource] ] : method development / / Richard A. Frazier, Jennifer M. Ames and Harry E. Nursten
Capillary electrophoresis for food analysis [[electronic resource] ] : method development / / Richard A. Frazier, Jennifer M. Ames and Harry E. Nursten
Autore Frazier Richard A
Pubbl/distr/stampa Cambridge, : Royal Society of Chemistry, 2000
Descrizione fisica 1 online resource (143 p.)
Disciplina 664.07
Altri autori (Persone) AmesJennifer M
NurstenHarry E
Soggetto topico Capillary electrophoresis
Food - Analysis
Food adulteration and inspection
ISBN 1-84755-031-2
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto OFC; BK9780854044924-FP001; BK9780854044924-FP005; BK9780854044924-FP007; BK9780854044924-FP011; BK9780854044924-00001; BK9780854044924-00008; BK9780854044924-00016; BK9780854044924-00025; BK9780854044924-00032; BK9780854044924-00047; BK9780854044924-00057; BK9780854044924-00061; BK9780854044924-00067; BK9780854044924-00088; BK9780854044924-00089; BK9780854044924-00095; BK9780854044924-00104; BK9780854044924-00113; BK9780854044924-00118
Record Nr. UNINA-9910778311403321
Frazier Richard A  
Cambridge, : Royal Society of Chemistry, 2000
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Chemometric methods in capillary electrophoresis [[electronic resource] /] / edited by Grady Hanrahan, Frank A. Gomez
Chemometric methods in capillary electrophoresis [[electronic resource] /] / edited by Grady Hanrahan, Frank A. Gomez
Pubbl/distr/stampa Hoboken, N.J., : Wiley, 2009
Descrizione fisica 1 online resource (460 p.)
Disciplina 660/.2972
Altri autori (Persone) HanrahanGrady
GomezFrank A
Soggetto topico Capillary electrophoresis
Chemometrics
ISBN 1-282-34832-9
9786612348327
0-470-53019-7
0-470-53018-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto CHEMOMETRIC METHODS IN CAPILLARY ELECTROPHORESIS; CONTENTS; PREFACE; ACKNOWLEDGMENTS; EDITOR BIOGRAPHIES; CONTRIBUTORS; KEY ACRONYMS; I EXPERIMENTAL DESIGN AND OPTIMIZATION CONSIDERATIONS; 1 INTRODUCTION; 2 EXPERIMENTAL DESIGN IN METHOD OPTIMIZATION AND ROBUSTNESS TESTING; 3 CHEMOMETRICAL EXPERIMENTAL DESIGN-BASED OPTIMIZATION STUDIES IN CAPILLARY ELECTROPHORESIS APPLICATIONS; 4 APPLICATION OF CHEMOMETRIC METHODS IN DRUG PURITY DETERMINATION BY CAPILLARY ELECTROPHORESIS; 5 OPTIMIZATION OF MICELLAR ELECTROKINETIC CHROMATOGRAPHY SEPARATION CONDITIONS BY CHEMOMETRIC METHODS
6 CHEMOMETRIC METHODS FOR THE OPTIMIZATION OF CE AND CE-MS IN PHARMACEUTICAL, ENVIRONMENTAL, AND FOOD ANALYSIS 7 OPTIMIZATION OF THE SEPARATION OF AMINO ACIDS BY CAPILLARY ELECTROPHORESIS USING ARTIFICIAL NEURAL NETWORKS; II EXPLORATORY DATA ANALYSIS, PREDICTION, AND CLASSIFICATION; 8 DEVELOPMENT OF CAPILLARY ELECTROPHORESIS FINGERPRINTS AND MULTIVARIATE STATISTICS FOR THE DIFFERENTIATION OF OPIUM AND POPPY STRAW SAMPLES; 9 MULTIVARIATE CURVE RESOLUTION BASED ON ALTERNATING LEAST SQUARES IN CAPILLARY ELECTROPHORESIS
10 APPLICATION OF CHEMOMETRICS IN CAPILLARY ELECTROPHORESIS ANALYSIS OF HERBAL MEDICINES 11 CLINICAL PATTERN RECOGNITION ANALYSIS APPLYING ARTIFICIAL NEURAL NETWORKS BASED ON PRINCIPAL COMPONENT ANALYSIS INPUT SELECTION; 12 CHEMOMETRIC METHODS APPLIED TO GENETIC ANALYSES BY CAPILLARY ELECTROPHORESIS AND ELECTROPHORESIS MICROCHIP TECHNOLOGIES; 13 EXPLORATORY DATA ANALYSIS AND CLASSIFICATION OF CAPILLARY ELECTROPHORETIC DATA; III QUANTITATIVE STRUCTURE RELATIONSHIPS; 14 CHEMOMETRICAL MODELING OF ELECTROPHORETIC MOBILITIES IN CAPILLARY ELECTROPHORESIS
15 ASSESSMENT OF SOLUTE-MICELLE INTERACTIONS IN ELECTROKINETIC CHROMATOGRAPHY USING QUANTITATIVE STRUCTURE-RETENTION RELATIONSHIPS 16 CHEMOMETRICAL ANALYSIS OF CHEESE PROTEOLYSIS PROFILES BY CAPILLARY ELECTROPHORESIS: PREDICTION OF RIPENING TIMES; IV TRANSFORMATION TECHNIQUES; 17 TRANSFORMATION TECHNIQUES FOR CAPILLARY AND MICROCHIP ELECTROPHORESIS; INDEX
Record Nr. UNINA-9910139869703321
Hoboken, N.J., : Wiley, 2009
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