Edizione	[3. ed.]
Pubbl/distr/stampa	London : Edward Arnold, 1986
Descrizione fisica	xix, 396 p. : ill. ; 23 cm
Disciplina	572
Altri autori (Persone)	Wilson, Keith Goulding, Kenneth H.
Collana	Contemporary biology
Soggetto topico	Biochemistry - Technique
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Record Nr.	UNISALENTO-991000236229707536

Autore	Fischer, Lave
Edizione	[2nd. ed.]
Pubbl/distr/stampa	Amsterdam ; New York : Elsevier/North-Holland Biomedical Press, 1980
Descrizione fisica	viii, 269 p. : ill. ; 20 cm
Disciplina	572
Collana	Laboratory techniques in biochemistry and molecular biology ; 1 Pt.2
Soggetto topico	Gel permeation chromatography Biochemistry - Technique Molecular biology - Technique Chromatography - laboratory manuals Electrophoresis Gels - laboratory manuals
ISBN	044480191X
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Record Nr.	UNISALENTO-991004051489707536

Pubbl/distr/stampa	[New York], : Elsevier
Soggetto topico	Biochemistry - Technique Biophysics - Technique Molecular biology - Technique Biochemistry - instrumentation Biochemistry - methods Biophysics - instrumentation Biophysics - methods Biochimie - Technique Biophysique - Technique Biologie moléculaire - Technique
Soggetto genere / forma	Periodicals.
Soggetto non controllato	Biophysics
ISSN	1872-857X
Formato	Materiale a stampa
Livello bibliografico	Periodico
Lingua di pubblicazione	eng
Record Nr.	UNISA-996218131103316

Pubbl/distr/stampa	Weinheim, : Wiley-VCH, c2006
Descrizione fisica	1 online resource (258 p.)
Disciplina	572.435
Altri autori (Persone)	DaunertSylvia DeoSapna K
Soggetto topico	Proteins Biomolecules - Analysis Biochemistry - Technique Photoluminescence Bioluminescence
Soggetto genere / forma	Electronic books.
ISBN	1-280-72271-1 9786610722716 3-527-60914-8 3-527-60869-9
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Nota di contenuto	Photoproteins in Bioanalysis; Contents; Preface; List of Contributors; 1 The Photoproteins; 1.1 Discovery of Photoprotein; 1.2 Various Types of Photoproteins Presently Known; 1.2.1 Radiolarian (Protozoa) Photoproteins; 1.2.2 Coelenterate Photoproteins; 1.2.3 Ctenophore Photoproteins; 1.2.4 Pholasin (Pholas Luciferin); 1.2.5 Chaetopterus Photoprotein; 1.2.6 Polynoidin; 1.2.7 Symplectin; 1.2.8 Luminodesmus Photoprotein; 1.2.9 Ophiopsila Photoprotein; 1.3 Basic Strategy of Extracting and Purifying Photoproteins; 1.4 The Photoprotein Aequorin; 1.4.1 Extraction and Purification of Aequorin 1.4.1.1 Hydrophobic Interaction Chromatography1.4.2 Properties of Aequorin; 1.4.2.1 Stability; 1.4.2.2 Freeze-drying; 1.4.3 Specificity to Ca(2+); 1.4.4 Luminescence of Aequorin by Substances Other Than Divalent Cations; 1.4.5 Mechanism of Aequorin Luminescence and Regeneration of Aequorin; 1.4.5.1 Structure of Aequorin; 1.4.5.2 Luminescence Reaction; 1.4.5.3 Regeneration; 1.4.6 Inhibitors of Aequorin Luminescence; 1.4.7 Recombinant Aequorin; 1.4.8 Semi-synthetic Aequorins; 1.4.8.1 e-Aequorins; References; 2 Luminous Marine Organisms; 2.1 Introduction; 2.1.1 Non-luminous Taxa 2.1.2 Luminous Taxa2.2 Taxonomic Distribution of Bioluminescence; 2.2.1 Bacterial Luminescence; 2.2.2 Dinoflagellate Luciferin; 2.2.3 Cypridina (Vargula) Luciferin; 2.2.4 Coelenterazine; 2.2.5 Other Luciferins: Known and Unknown; 2.3 Functions; 2.3.1 Startle or Distract; 2.3.2 Burglar Alarm; 2.3.3 Counterillumination; 2.3.4 Mating Displays; 2.3.5 Prey Attraction; References; 3 Beetle Luciferases: Colorful Lights on Biological Processes and Diseases; 3.1 Introduction; 3.2 Beetle Luciferases; 3.3 Bioanalytical Assays of ATP; 3.3.1 Biomass Estimation and Microbiological Contamination 3.3.2 Cytotoxicity and Cell Viability Tests3.3.3 Enzymatic Assays; 3.4 Luciferases as Reporter Genes; 3.4.1 Dual and Multiple Reporter Assays; 3.5 Biophotonic Imaging in Animals: A Living Light on Diseases; 3.5.1 Pathogen Infection in Living Models; 3.5.2 Drug Screening; 3.5.3 Tumor Proliferation and Regression Studies; 3.5.4 Gene Delivery and Gene Therapy; 3.5.5 Luciferase as Biomarkers for Cell Trafficking Studies; 3.5.6 Immunoassays; 3.6 Biophotonic Imaging in Plants; 3.7 Biosensors: Sensing the Environment; 3.8 Novel Luciferases: Different Colors for Different Occasions; References 4 Split Luciferase Systems for Detecting Protein-Protein Interactions in Mammalian Cells Based on Protein Splicing and Protein Complementation4.1 Introduction; 4.2 Protein Splicing-based Split Firefly Luciferase System [23]; 4.2.1 Split Luciferase Works as a Probe for Protein Interaction; 4.3 Split Renilla Luciferase Complementation System [33]; 4.3.1 Time Course of the Interaction Between Y941 and SH2n; 4.3.2 Location of the Interaction Between Y941 and SH2n; References; 5 Photoproteins in Nucleic Acid Analysis; 5.1 Hybridization Assays; 5.2 Quantitative Polymerase Chain Reaction 5.3 Genotyping of Single-nucleotide Polymorphisms
Record Nr.	UNINA-9910144144403321

Pubbl/distr/stampa	New York : Academic press, 1955
Descrizione fisica	2 v : ill. ; 24 cm
Disciplina	572
Altri autori (Persone)	Kaplan, Nathan Oram Colowick, Sidney P.
Collana	Methods in enzymology ; 001 Methods in enzymology ; 002
Soggetto topico	Enzymes Biochemistry - Technique
Formato	Materiale a stampa
Livello bibliografico	Monografia
Lingua di pubblicazione	eng
Record Nr.	UNISALENTO-991003266629707536