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Biblioteca

MARC Lista (tabellare)
Advances and challenges in the delivery of nucleic acid therapeutics . Volume 1 / / editors, Olivia Merkel, Mansoor Amiji
Advances and challenges in the delivery of nucleic acid therapeutics . Volume 1 / / editors, Olivia Merkel, Mansoor Amiji
Pubbl/distr/stampa London, England : , : Future Science Ltd, , 2015
Descrizione fisica 1 online resource (136 pages) : illustrations (some color)
Disciplina 572.8
Soggetto topico Antisense nucleic acids
Nucleic acids
Soggetto genere / forma Electronic books.
ISBN 1-910419-93-1
1-910419-92-3
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910464376103321
London, England : , : Future Science Ltd, , 2015
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Advances and challenges in the delivery of nucleic acid therapeutics . Volume 2. / / editors, Olivia Merkel, Mansoor Amiji
Advances and challenges in the delivery of nucleic acid therapeutics . Volume 2. / / editors, Olivia Merkel, Mansoor Amiji
Pubbl/distr/stampa London, England : , : Future Science Ltd, , 2015
Descrizione fisica 1 online resource (129 pages) : illustrations (some color)
Disciplina 572.8
Soggetto topico Antisense nucleic acids
Nucleic acids
Soggetto genere / forma Electronic books.
ISBN 1-910419-96-6
1-910419-95-8
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910464375403321
London, England : , : Future Science Ltd, , 2015
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Advances and challenges in the delivery of nucleic acid therapeutics . Volume 2. / / editors, Olivia Merkel, Mansoor Amiji
Advances and challenges in the delivery of nucleic acid therapeutics . Volume 2. / / editors, Olivia Merkel, Mansoor Amiji
Pubbl/distr/stampa London, England : , : Future Science Ltd, , 2015
Descrizione fisica 1 online resource (129 pages) : illustrations (some color)
Disciplina 572.8
Soggetto topico Antisense nucleic acids
Nucleic acids
ISBN 1-910419-96-6
1-910419-95-8
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910789501003321
London, England : , : Future Science Ltd, , 2015
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Advances and challenges in the delivery of nucleic acid therapeutics . Volume 1 / / editors, Olivia Merkel, Mansoor Amiji
Advances and challenges in the delivery of nucleic acid therapeutics . Volume 1 / / editors, Olivia Merkel, Mansoor Amiji
Pubbl/distr/stampa London, England : , : Future Science Ltd, , 2015
Descrizione fisica 1 online resource (136 pages) : illustrations (some color)
Disciplina 572.8
Soggetto topico Antisense nucleic acids
Nucleic acids
ISBN 1-910419-93-1
1-910419-92-3
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910789499403321
London, England : , : Future Science Ltd, , 2015
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Advances and challenges in the delivery of nucleic acid therapeutics . Volume 1 / / editors, Olivia Merkel, Mansoor Amiji
Advances and challenges in the delivery of nucleic acid therapeutics . Volume 1 / / editors, Olivia Merkel, Mansoor Amiji
Pubbl/distr/stampa London, England : , : Future Science Ltd, , 2015
Descrizione fisica 1 online resource (136 pages) : illustrations (some color)
Disciplina 572.8
Soggetto topico Antisense nucleic acids
Nucleic acids
ISBN 1-910419-93-1
1-910419-92-3
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910827771803321
London, England : , : Future Science Ltd, , 2015
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Advances and challenges in the delivery of nucleic acid therapeutics . Volume 2. / / editors, Olivia Merkel, Mansoor Amiji
Advances and challenges in the delivery of nucleic acid therapeutics . Volume 2. / / editors, Olivia Merkel, Mansoor Amiji
Pubbl/distr/stampa London, England : , : Future Science Ltd, , 2015
Descrizione fisica 1 online resource (129 pages) : illustrations (some color)
Disciplina 572.8
Soggetto topico Antisense nucleic acids
Nucleic acids
ISBN 1-910419-96-6
1-910419-95-8
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910827671003321
London, England : , : Future Science Ltd, , 2015
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Antisense and ribozyme methodology / / Ian Gibson, editor
Antisense and ribozyme methodology / / Ian Gibson, editor
Pubbl/distr/stampa London, [England] : , : Chapman & Hall, , 1997
Descrizione fisica 1 online resource (96 p.)
Disciplina 572.88
615.36
Collana Laboratory Companion Series
Soggetto topico Antisense nucleic acids
Catalytic RNA
Soggetto genere / forma Electronic books.
ISBN 1-281-84239-7
9786611842390
3-527-61253-X
3-527-61252-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Antisense and Ribozyme Methodology; Contents; CHAPTER 1. Antisense and Ribozyme Methodology; 1.1 The Potential; 1.2 Antisense Technology; 1.2.1 Problems; 1.2.2 Resistance to Nucleases; 1.2.3 Entry into Cells; 1.2.4 How Antisense Works; 1.2.5 Success; 1.3 Ribozymes; 1.3.1 What Are They?; 1.3.2 Problems; 1.3.3 Stable Ribozymes; 1.3.4 Designing Ribozymes; 1.4 Ribozymes or Antisense DNAs?; 1.5 The Choice Today!!; CHAPTER 2. Design and Synthesis of Antisense DNA Molecules; 2.1 Introduction; 2.2 Synthesis of Methylphosphonodiester-Phosphodiester Chimeric Oligodeoxynucleotides
2.2.1 Materials and Chemicals2.2.2 Solutions; 2.2.3 Maximizing Product Purity; 2.2.4 Deprotection of Chimeric Oligodeoxynucleotides; 2.2.5 Failed Sequences; 2.3 Primary Purification by Reversed-Phase, Solid-Phase Extraction on C18 SEP-PAK Cartridges; 2.3.1 Equipment; 2.3.2 Method; 2.3.3 Purification of the Oligodeoxynucleotide; 2.3.4 Further Purification; 2.4 Analysis and Purification by HPLC; 2.4.1 Analysis of Chimeric Oligodeoxynucleotides by HPLC; 2.4.2 Purification of Chimeric Oligodeoxynucleotides by HPLC; 2.4.3 Re-Use of Columns
2.5 Synthesis of Chimeric Oligodeoxynucleotides with Fluorescein Attached2.6 Summary; CHAPTER 3. The Design and Synthesis of Hammerhead Ribozymes; 3.1 Introduction; 3.2 The Design of Hammerhead Ribozymes; 3.3 Improving the Reactions; 3.3.1 Accessibility of the Target - Substrate Binding; 3.3.2 Finding the Target; 3.3.3 Theoretical Considerations; 3.3.4 Experimental Approaches; 3.3.5 Kinetic Studies; 3.4 Length of Arms; 3.4.1 Choosing Antisense Arms of Hammerhead Ribozymes; 3.4.2 Arms of Different Lengths; 3.5 Cleavage of the Target Motif; 3.6 Synthesis of Ribozymes
3.6.1 Chemical Synthesis of Short Hammerhead Ribozymes3.6.2 Enzymatic Transcription in Vitro; 3.7 Endogenous Expression of Ribozyme Genes; CHAPTER 4. Delivery of Ribozymes and Antisense DNA Molecules into Mammalian Cells; 4.1 Introduction; 4.2 Exogenous Application; 4.2.1 lntracytoplasmic Delivery of Antisense Oligodeoxynucleotides by Reversible Plasma Membrane Permeabilization with Streptolysin O; 4.3 Microinjection; 4.4 Other Methods Used in Nucleic Acid Transfection; 4.5 Electroporation; 4.5.1 Method; 4.5.2 Transfection: Optimization of Conditions
4.5.3 Mechanism of Uptake Following Electroporation4.5.4 Benefits and Drawbacks of Electrophoretic-Mediated Uptake; 4.6 Diethylaminoethyl-Dextran (DEAE- Dextran) and DNA Transfection; 4.6.1 Methods for Transfection of Adherent Cells; 4.6.2 Possible Alterations of the Above Protocol; 4.6.3 Transfection of Cells Growing in Suspension; 4.6.4 Transfection Optimization; 4.6.5 Distribution Mechanism of DEAE-Dextran Uptake and lntracellular; 4.6.6 Benefits and Drawbacks; 4.7 Calcium Phosphate Transfection; 4.7.1 Method; 4.7.2 Possible Alterations to Above Method; 4.7.3 Method Optimization
4.7.4 Calcium Phosphate-Mediated Uptake and lntracellular Distribution
Record Nr. UNINA-9910144117903321
London, [England] : , : Chapman & Hall, , 1997
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Antisense and ribozyme methodology / / Ian Gibson, editor
Antisense and ribozyme methodology / / Ian Gibson, editor
Pubbl/distr/stampa London, [England] : , : Chapman & Hall, , 1997
Descrizione fisica 1 online resource (96 p.)
Disciplina 572.88
615.36
Collana Laboratory Companion Series
Soggetto topico Antisense nucleic acids
Catalytic RNA
ISBN 1-281-84239-7
9786611842390
3-527-61253-X
3-527-61252-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Antisense and Ribozyme Methodology; Contents; CHAPTER 1. Antisense and Ribozyme Methodology; 1.1 The Potential; 1.2 Antisense Technology; 1.2.1 Problems; 1.2.2 Resistance to Nucleases; 1.2.3 Entry into Cells; 1.2.4 How Antisense Works; 1.2.5 Success; 1.3 Ribozymes; 1.3.1 What Are They?; 1.3.2 Problems; 1.3.3 Stable Ribozymes; 1.3.4 Designing Ribozymes; 1.4 Ribozymes or Antisense DNAs?; 1.5 The Choice Today!!; CHAPTER 2. Design and Synthesis of Antisense DNA Molecules; 2.1 Introduction; 2.2 Synthesis of Methylphosphonodiester-Phosphodiester Chimeric Oligodeoxynucleotides
2.2.1 Materials and Chemicals2.2.2 Solutions; 2.2.3 Maximizing Product Purity; 2.2.4 Deprotection of Chimeric Oligodeoxynucleotides; 2.2.5 Failed Sequences; 2.3 Primary Purification by Reversed-Phase, Solid-Phase Extraction on C18 SEP-PAK Cartridges; 2.3.1 Equipment; 2.3.2 Method; 2.3.3 Purification of the Oligodeoxynucleotide; 2.3.4 Further Purification; 2.4 Analysis and Purification by HPLC; 2.4.1 Analysis of Chimeric Oligodeoxynucleotides by HPLC; 2.4.2 Purification of Chimeric Oligodeoxynucleotides by HPLC; 2.4.3 Re-Use of Columns
2.5 Synthesis of Chimeric Oligodeoxynucleotides with Fluorescein Attached2.6 Summary; CHAPTER 3. The Design and Synthesis of Hammerhead Ribozymes; 3.1 Introduction; 3.2 The Design of Hammerhead Ribozymes; 3.3 Improving the Reactions; 3.3.1 Accessibility of the Target - Substrate Binding; 3.3.2 Finding the Target; 3.3.3 Theoretical Considerations; 3.3.4 Experimental Approaches; 3.3.5 Kinetic Studies; 3.4 Length of Arms; 3.4.1 Choosing Antisense Arms of Hammerhead Ribozymes; 3.4.2 Arms of Different Lengths; 3.5 Cleavage of the Target Motif; 3.6 Synthesis of Ribozymes
3.6.1 Chemical Synthesis of Short Hammerhead Ribozymes3.6.2 Enzymatic Transcription in Vitro; 3.7 Endogenous Expression of Ribozyme Genes; CHAPTER 4. Delivery of Ribozymes and Antisense DNA Molecules into Mammalian Cells; 4.1 Introduction; 4.2 Exogenous Application; 4.2.1 lntracytoplasmic Delivery of Antisense Oligodeoxynucleotides by Reversible Plasma Membrane Permeabilization with Streptolysin O; 4.3 Microinjection; 4.4 Other Methods Used in Nucleic Acid Transfection; 4.5 Electroporation; 4.5.1 Method; 4.5.2 Transfection: Optimization of Conditions
4.5.3 Mechanism of Uptake Following Electroporation4.5.4 Benefits and Drawbacks of Electrophoretic-Mediated Uptake; 4.6 Diethylaminoethyl-Dextran (DEAE- Dextran) and DNA Transfection; 4.6.1 Methods for Transfection of Adherent Cells; 4.6.2 Possible Alterations of the Above Protocol; 4.6.3 Transfection of Cells Growing in Suspension; 4.6.4 Transfection Optimization; 4.6.5 Distribution Mechanism of DEAE-Dextran Uptake and lntracellular; 4.6.6 Benefits and Drawbacks; 4.7 Calcium Phosphate Transfection; 4.7.1 Method; 4.7.2 Possible Alterations to Above Method; 4.7.3 Method Optimization
4.7.4 Calcium Phosphate-Mediated Uptake and lntracellular Distribution
Record Nr. UNISA-996203927503316
London, [England] : , : Chapman & Hall, , 1997
Materiale a stampa
Lo trovi qui: Univ. di Salerno
Opac: Controlla la disponibilità qui
Antisense and ribozyme methodology / / Ian Gibson, editor
Antisense and ribozyme methodology / / Ian Gibson, editor
Pubbl/distr/stampa London, [England] : , : Chapman & Hall, , 1997
Descrizione fisica 1 online resource (96 p.)
Disciplina 572.88
615.36
Collana Laboratory Companion Series
Soggetto topico Antisense nucleic acids
Catalytic RNA
ISBN 1-281-84239-7
9786611842390
3-527-61253-X
3-527-61252-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Antisense and Ribozyme Methodology; Contents; CHAPTER 1. Antisense and Ribozyme Methodology; 1.1 The Potential; 1.2 Antisense Technology; 1.2.1 Problems; 1.2.2 Resistance to Nucleases; 1.2.3 Entry into Cells; 1.2.4 How Antisense Works; 1.2.5 Success; 1.3 Ribozymes; 1.3.1 What Are They?; 1.3.2 Problems; 1.3.3 Stable Ribozymes; 1.3.4 Designing Ribozymes; 1.4 Ribozymes or Antisense DNAs?; 1.5 The Choice Today!!; CHAPTER 2. Design and Synthesis of Antisense DNA Molecules; 2.1 Introduction; 2.2 Synthesis of Methylphosphonodiester-Phosphodiester Chimeric Oligodeoxynucleotides
2.2.1 Materials and Chemicals2.2.2 Solutions; 2.2.3 Maximizing Product Purity; 2.2.4 Deprotection of Chimeric Oligodeoxynucleotides; 2.2.5 Failed Sequences; 2.3 Primary Purification by Reversed-Phase, Solid-Phase Extraction on C18 SEP-PAK Cartridges; 2.3.1 Equipment; 2.3.2 Method; 2.3.3 Purification of the Oligodeoxynucleotide; 2.3.4 Further Purification; 2.4 Analysis and Purification by HPLC; 2.4.1 Analysis of Chimeric Oligodeoxynucleotides by HPLC; 2.4.2 Purification of Chimeric Oligodeoxynucleotides by HPLC; 2.4.3 Re-Use of Columns
2.5 Synthesis of Chimeric Oligodeoxynucleotides with Fluorescein Attached2.6 Summary; CHAPTER 3. The Design and Synthesis of Hammerhead Ribozymes; 3.1 Introduction; 3.2 The Design of Hammerhead Ribozymes; 3.3 Improving the Reactions; 3.3.1 Accessibility of the Target - Substrate Binding; 3.3.2 Finding the Target; 3.3.3 Theoretical Considerations; 3.3.4 Experimental Approaches; 3.3.5 Kinetic Studies; 3.4 Length of Arms; 3.4.1 Choosing Antisense Arms of Hammerhead Ribozymes; 3.4.2 Arms of Different Lengths; 3.5 Cleavage of the Target Motif; 3.6 Synthesis of Ribozymes
3.6.1 Chemical Synthesis of Short Hammerhead Ribozymes3.6.2 Enzymatic Transcription in Vitro; 3.7 Endogenous Expression of Ribozyme Genes; CHAPTER 4. Delivery of Ribozymes and Antisense DNA Molecules into Mammalian Cells; 4.1 Introduction; 4.2 Exogenous Application; 4.2.1 lntracytoplasmic Delivery of Antisense Oligodeoxynucleotides by Reversible Plasma Membrane Permeabilization with Streptolysin O; 4.3 Microinjection; 4.4 Other Methods Used in Nucleic Acid Transfection; 4.5 Electroporation; 4.5.1 Method; 4.5.2 Transfection: Optimization of Conditions
4.5.3 Mechanism of Uptake Following Electroporation4.5.4 Benefits and Drawbacks of Electrophoretic-Mediated Uptake; 4.6 Diethylaminoethyl-Dextran (DEAE- Dextran) and DNA Transfection; 4.6.1 Methods for Transfection of Adherent Cells; 4.6.2 Possible Alterations of the Above Protocol; 4.6.3 Transfection of Cells Growing in Suspension; 4.6.4 Transfection Optimization; 4.6.5 Distribution Mechanism of DEAE-Dextran Uptake and lntracellular; 4.6.6 Benefits and Drawbacks; 4.7 Calcium Phosphate Transfection; 4.7.1 Method; 4.7.2 Possible Alterations to Above Method; 4.7.3 Method Optimization
4.7.4 Calcium Phosphate-Mediated Uptake and lntracellular Distribution
Record Nr. UNINA-9910830630503321
London, [England] : , : Chapman & Hall, , 1997
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Pharmaceutical innovation
Pharmaceutical innovation
Pubbl/distr/stampa Montclair, N.J. : , : Adis International, Inc.
Disciplina 338
Soggetto topico Pharmaceutical technology
Pharmaceutical industry
Gene therapy
Antisense nucleic acids
Soggetto genere / forma Periodicals.
ISSN 2377-6625
Formato Materiale a stampa
Livello bibliografico Periodico
Lingua di pubblicazione eng
Record Nr. UNISA-996204782703316
Montclair, N.J. : , : Adis International, Inc.
Materiale a stampa
Lo trovi qui: Univ. di Salerno
Opac: Controlla la disponibilità qui