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Essential molecular biology : a practical approach / edited by T. A. Brown
| Essential molecular biology : a practical approach / edited by T. A. Brown |
| Pubbl/distr/stampa | Oxford ; New York, : IRL Press at Oxford University Press, c1991 |
| Descrizione fisica | 2 v. (XIX, 299; 296 p.) 23 cm : ill. ; 24 cm |
| Disciplina |
574.88
572.8 |
| Collana | The practical approach series |
| Soggetto non controllato | Biologia molecolare |
| ISBN |
0-19-963111-5
0-19-963113-1 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-990002224690403321 |
| Oxford ; New York, : IRL Press at Oxford University Press, c1991 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Gene probes : a practical approach / edited by B. D. Hames, S.J. Higgins
| Gene probes : a practical approach / edited by B. D. Hames, S.J. Higgins |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Oxford ; New York : IRL Press at Oxford University Press, c1995 |
| Descrizione fisica | 2 v. : ill. ; 24 cm |
| Disciplina | 572.86 |
| Altri autori (Persone) |
Hames, B. D.
Higgins, S. J. (Steve J.) |
| Collana | The practical approach series |
| Soggetto topico | DNA probes |
| ISBN |
0199634009 (v.1)
0199634149 (v.2) |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991001135049707536 |
| Oxford ; New York : IRL Press at Oxford University Press, c1995 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
| ||
Immunocyto-chemistry : a practical approach / edited by Julian E. Beesley
| Immunocyto-chemistry : a practical approach / edited by Julian E. Beesley |
| Pubbl/distr/stampa | Oxford ; New York ; Tokyo : IRL Press at Oxford University Press, c1993 |
| Descrizione fisica | XVIII, 248 p. : ill. ; 24 cm |
| Collana | The practical approach |
| Soggetto non controllato | Chimica |
| ISBN | 0199632693 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-990008852130403321 |
| Oxford ; New York ; Tokyo : IRL Press at Oxford University Press, c1993 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
The Macrophage / edited by Claire E. Lewis and James O'D. McGee
| The Macrophage / edited by Claire E. Lewis and James O'D. McGee |
| Pubbl/distr/stampa | Oxford ; New York : IRL Press at Oxford University Press, c1992 |
| Descrizione fisica | xxii, 423 p. : ill. ; 24 cm |
| Disciplina | 616.079 |
| Altri autori (Persone) |
Lewis, Claire E.
McGee, James O'Donnell |
| Collana | The Natural immune system |
| Soggetto topico |
Bacterial Infections - Immunology
Hematopoiesis - Immunology Immunity, Natural Macrophages - Immunology Parasitic Diseases - Immunology Virus Diseases - Immunology |
| ISBN | 0199632340 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991000700899707536 |
| Oxford ; New York : IRL Press at Oxford University Press, c1992 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
| ||
Microcomputers in biology [Risorsa elettronica] : BASIC software for the IBM PC / ed. by C.R. Ireland, S.P. Long
| Microcomputers in biology [Risorsa elettronica] : BASIC software for the IBM PC / ed. by C.R. Ireland, S.P. Long |
| Pubbl/distr/stampa | Oxford : IRL Press at Oxford University Press, c1985 |
| Descrizione fisica | 2 dischi in cofanetto ; 5 1/4 pollici |
| Soggetto non controllato |
Microcomputer
Biologia |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-990009416100403321 |
| Oxford : IRL Press at Oxford University Press, c1985 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Mutation detection : a practical approach / / edited by R.G.H. Cotton, E. Edkins and S. Forrest [[electronic resource]]
| Mutation detection : a practical approach / / edited by R.G.H. Cotton, E. Edkins and S. Forrest [[electronic resource]] |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Oxford : , : IRL Press at Oxford University Press, , 2023 |
| Descrizione fisica | 1 online resource (263 p.) |
| Disciplina | 576.5/49 |
| Collana |
Practical approach series
Oxford scholarship online Practical approach series |
| Soggetto topico |
Mutation (Biology)
Molecular genetics Chromosome abnormalities |
| ISBN |
1-383-04935-1
0-19-156569-5 1-280-37548-5 9786610375486 0-585-48413-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Contents; List of Contributors; Abbreviations; Introduction; References; 1. Single-strand conformation polymorphism analysis; 1. Introduction; 2. PCR-SSCP using polyacrylamide slab gel; PCR Optimization and primer design; Pre-amplification and isolation by agarose gel electrophoresis; PCR using [[Sup(32)]P]deoxynucleotide triphosphate; Removal of 3' appendage; SSCP gel electrophoresis; Interpretation of autoradiogram; Re-amplification and direct sequencing; Gel matrices other than polyacrylamide; Restriction endonuclease fingerprinting and dideoxy fingerprinting
3. Fluorescent SSCP in an automated DNA sequencerPrimer design in post-PCR fluorescent labelling; Fluorescent labelling by 3' exchange reaction; SSCP in capillary electrophoresis (CE-SSCP); Data processing; Acknowledgements; References; 2. Single-stranded conformation polymorphism and heteroduplex analysis; 1. Introduction; 2. Optimization of the PCR reaction; 3. SSCP sample prepration; 4. Optimization of SSCP/HA detection; 5. Multiplexing; 6. Interpretation of results; 7. Applications; 8. Other methods; References 3. Comprehensive mutation detection with denaturing gradient gel electrophoresis1. Introduction; The scope of DGGE, its distinctive capabilities, and the nature of results; 2. Background; 3. Basic principle, the physical properties of DNA; 4. Overview of the procedures in searching for mutants; Defining segments for scrutiny; Sample preparation; Gradient and velocity separations; Features of the gel patterns; Discrimination of zygozygosity; Comments; 5. Use of the psoralen cross-link as a clamp; The psoralen protocol; 6. Computational tools; What is a meltmap?; Meltmap protocol Predicting electrophoretic separationsComputer operations for MUTRAV; 7. Other members of the DGGE family; Gel separations in a uniform, partially denaturing environment; Capillary electrophoresis; The thermal gradient; The temperature ramp; 2D length and gradient separations; 8. End notes; Acknowledgments; References; 4. Cleavage using RNase to detect mutations; 1. Introduction; 2. RNase protection assay for mutation detection; Evaluation of the sensitivity; Source material; PCR for RNase protection assay; RNA probe preparation; RNase protection; Detection of digested probe Mutation detection by sequencing of the PCR productsOther modified methodologies for mutation detection; Acknowledgements; References; 5. Cleavage of mismatched bases using chemical reagents; 1. Introduction; 2. Basic procedures; Comments on the basic procedures; 3. Ultra fast chemical mismatch detection; Labelling; Solid phase; Comments; References; 6. Mutation detection using T4 endonuclease VII; 1. Introduction; 2. The biology of Endo VII; The role of Endo VII in vivo; Characterization of Endo VII; Action of Endo VII on heteroduplex DNA; 3. Use of Endo VII for mutation detection Enzyme mismatch cleavage |
| Record Nr. | UNINA-9910815297603321 |
| Oxford : , : IRL Press at Oxford University Press, , 2023 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Mutation detection [[electronic resource] ] : a practical approach / / edited by R.G.H. Cotton, E. Edkins, and S. Forrest
| Mutation detection [[electronic resource] ] : a practical approach / / edited by R.G.H. Cotton, E. Edkins, and S. Forrest |
| Pubbl/distr/stampa | Oxford ; ; New York, : IRL Press at Oxford University Press, 1998 |
| Descrizione fisica | 1 online resource (263 p.) |
| Disciplina | 576.5/49 |
| Altri autori (Persone) |
CottonRichard G. H
EdkinsE (Edward) ForrestS (Sue) |
| Collana | Practical approach series |
| Soggetto topico |
Mutation (Biology)
Molecular genetics Chromosome abnormalities |
| Soggetto genere / forma | Electronic books. |
| ISBN |
0-19-156569-5
1-280-37548-5 9786610375486 0-585-48413-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Contents; List of Contributors; Abbreviations; Introduction; References; 1. Single-strand conformation polymorphism analysis; 1. Introduction; 2. PCR-SSCP using polyacrylamide slab gel; PCR Optimization and primer design; Pre-amplification and isolation by agarose gel electrophoresis; PCR using [[Sup(32)]P]deoxynucleotide triphosphate; Removal of 3' appendage; SSCP gel electrophoresis; Interpretation of autoradiogram; Re-amplification and direct sequencing; Gel matrices other than polyacrylamide; Restriction endonuclease fingerprinting and dideoxy fingerprinting
3. Fluorescent SSCP in an automated DNA sequencerPrimer design in post-PCR fluorescent labelling; Fluorescent labelling by 3' exchange reaction; SSCP in capillary electrophoresis (CE-SSCP); Data processing; Acknowledgements; References; 2. Single-stranded conformation polymorphism and heteroduplex analysis; 1. Introduction; 2. Optimization of the PCR reaction; 3. SSCP sample prepration; 4. Optimization of SSCP/HA detection; 5. Multiplexing; 6. Interpretation of results; 7. Applications; 8. Other methods; References 3. Comprehensive mutation detection with denaturing gradient gel electrophoresis1. Introduction; The scope of DGGE, its distinctive capabilities, and the nature of results; 2. Background; 3. Basic principle, the physical properties of DNA; 4. Overview of the procedures in searching for mutants; Defining segments for scrutiny; Sample preparation; Gradient and velocity separations; Features of the gel patterns; Discrimination of zygozygosity; Comments; 5. Use of the psoralen cross-link as a clamp; The psoralen protocol; 6. Computational tools; What is a meltmap?; Meltmap protocol Predicting electrophoretic separationsComputer operations for MUTRAV; 7. Other members of the DGGE family; Gel separations in a uniform, partially denaturing environment; Capillary electrophoresis; The thermal gradient; The temperature ramp; 2D length and gradient separations; 8. End notes; Acknowledgments; References; 4. Cleavage using RNase to detect mutations; 1. Introduction; 2. RNase protection assay for mutation detection; Evaluation of the sensitivity; Source material; PCR for RNase protection assay; RNA probe preparation; RNase protection; Detection of digested probe Mutation detection by sequencing of the PCR productsOther modified methodologies for mutation detection; Acknowledgements; References; 5. Cleavage of mismatched bases using chemical reagents; 1. Introduction; 2. Basic procedures; Comments on the basic procedures; 3. Ultra fast chemical mismatch detection; Labelling; Solid phase; Comments; References; 6. Mutation detection using T4 endonuclease VII; 1. Introduction; 2. The biology of Endo VII; The role of Endo VII in vivo; Characterization of Endo VII; Action of Endo VII on heteroduplex DNA; 3. Use of Endo VII for mutation detection Enzyme mismatch cleavage |
| Record Nr. | UNINA-9910455737203321 |
| Oxford ; ; New York, : IRL Press at Oxford University Press, 1998 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Mutation detection [[electronic resource] ] : a practical approach / / edited by R.G.H. Cotton, E. Edkins, and S. Forrest
| Mutation detection [[electronic resource] ] : a practical approach / / edited by R.G.H. Cotton, E. Edkins, and S. Forrest |
| Pubbl/distr/stampa | Oxford ; ; New York, : IRL Press at Oxford University Press, 1998 |
| Descrizione fisica | 1 online resource (263 p.) |
| Disciplina | 576.5/49 |
| Altri autori (Persone) |
CottonRichard G. H
EdkinsE (Edward) ForrestS (Sue) |
| Collana | Practical approach series |
| Soggetto topico |
Mutation (Biology)
Molecular genetics Chromosome abnormalities |
| ISBN |
1-383-04935-1
0-19-156569-5 1-280-37548-5 9786610375486 0-585-48413-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Contents; List of Contributors; Abbreviations; Introduction; References; 1. Single-strand conformation polymorphism analysis; 1. Introduction; 2. PCR-SSCP using polyacrylamide slab gel; PCR Optimization and primer design; Pre-amplification and isolation by agarose gel electrophoresis; PCR using [[Sup(32)]P]deoxynucleotide triphosphate; Removal of 3' appendage; SSCP gel electrophoresis; Interpretation of autoradiogram; Re-amplification and direct sequencing; Gel matrices other than polyacrylamide; Restriction endonuclease fingerprinting and dideoxy fingerprinting
3. Fluorescent SSCP in an automated DNA sequencerPrimer design in post-PCR fluorescent labelling; Fluorescent labelling by 3' exchange reaction; SSCP in capillary electrophoresis (CE-SSCP); Data processing; Acknowledgements; References; 2. Single-stranded conformation polymorphism and heteroduplex analysis; 1. Introduction; 2. Optimization of the PCR reaction; 3. SSCP sample prepration; 4. Optimization of SSCP/HA detection; 5. Multiplexing; 6. Interpretation of results; 7. Applications; 8. Other methods; References 3. Comprehensive mutation detection with denaturing gradient gel electrophoresis1. Introduction; The scope of DGGE, its distinctive capabilities, and the nature of results; 2. Background; 3. Basic principle, the physical properties of DNA; 4. Overview of the procedures in searching for mutants; Defining segments for scrutiny; Sample preparation; Gradient and velocity separations; Features of the gel patterns; Discrimination of zygozygosity; Comments; 5. Use of the psoralen cross-link as a clamp; The psoralen protocol; 6. Computational tools; What is a meltmap?; Meltmap protocol Predicting electrophoretic separationsComputer operations for MUTRAV; 7. Other members of the DGGE family; Gel separations in a uniform, partially denaturing environment; Capillary electrophoresis; The thermal gradient; The temperature ramp; 2D length and gradient separations; 8. End notes; Acknowledgments; References; 4. Cleavage using RNase to detect mutations; 1. Introduction; 2. RNase protection assay for mutation detection; Evaluation of the sensitivity; Source material; PCR for RNase protection assay; RNA probe preparation; RNase protection; Detection of digested probe Mutation detection by sequencing of the PCR productsOther modified methodologies for mutation detection; Acknowledgements; References; 5. Cleavage of mismatched bases using chemical reagents; 1. Introduction; 2. Basic procedures; Comments on the basic procedures; 3. Ultra fast chemical mismatch detection; Labelling; Solid phase; Comments; References; 6. Mutation detection using T4 endonuclease VII; 1. Introduction; 2. The biology of Endo VII; The role of Endo VII in vivo; Characterization of Endo VII; Action of Endo VII on heteroduplex DNA; 3. Use of Endo VII for mutation detection Enzyme mismatch cleavage |
| Record Nr. | UNINA-9910780115803321 |
| Oxford ; ; New York, : IRL Press at Oxford University Press, 1998 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Subcellular fractionation [[electronic resource] ] : a practical approach / / edited by J.M. Graham and D. Rickwood
| Subcellular fractionation [[electronic resource] ] : a practical approach / / edited by J.M. Graham and D. Rickwood |
| Pubbl/distr/stampa | Oxford ; ; New York, : IRL Press at Oxford University Press, c1997 |
| Descrizione fisica | 1 online resource (360 p.) |
| Disciplina | 571.6/5 |
| Altri autori (Persone) |
GrahamJ. M <1943-> (John M.)
RickwoodD (David) |
| Collana | The practical approach series |
| Soggetto topico | Subcellular fractionation |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-283-66474-7
0-19-159161-0 0-585-48392-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Contents; List of contributors; Abbreviations; 1. Homogenization of tissues and cells; 1. Introduction; 2. Aims of the homogenization procedure; 3. Influence of sample type; 4. Homogenization media; 5. Methods of homogenization; Type 1 homogenizers; Type 2 homogenizers; 6. Homogenization of tissues and cells; Mammalian liver; Brain; Muscle; Mammalian tissue culture cells; Plant organelles; Yeast; Other fungi and algae; Trypanosomes; Bacteria; References; 2. Isolation of subcellular fractions; 1. Introduction; 2. Composition of a tissue homogenate; 3. Properties of cell organelles
Factors affecting organelle density and size4. Centrifugal methods for the separation of organelles; Separation by size; Separation by density; Density perturbation; 5. Non-centrifugal procedures; Immunoisolation; Separation by electrophoresis; 6. Identification of separated material; Marker enzymes; Introduced markers for endocytic and exocytic pathways; Characteristic non-enzymatic proteins; 7. Assessment of the purity of fractions; Purity and purification; Problems from cell heterogeneity within tissues; Problems arising from organelle fragmentation Missorting in the exocytic and endocytic pathways8. Fractionation problems; No separation; Aggregation following resuspension of a fraction; Poor recovery of markers; Damage to cell structures; 9. A systematic approach to cell fractionation; Preliminary studies; Determination of the properties of components of the homogenate; Method development; Simplification of the separation; References; 3. Isolation and characterization of nuclei and nuclear subfractions; 1. Introduction; 2. Methods of preparing purified nuclei; Types of cells and tissue samples; Homogenization media Homogenization methodsCentrifugation conditions; Assays of nuclear purity; 3. Methods for purifying metaphase chromosomes; 4. Isolation of nuclear subfractions; Preparation of nucleoli; Preparation of nuclear membranes; Isolation of nuclear matrix; Preparation of nucleoids; 5. Isolation of nucleoprotein complexes; Isolation of polynucleosomes of chromatin; Ribonucleoproteins; 6. Isolation of nuclear macromolecules; Isolation of nuclear proteins; Isolation of nuclear RNA; Isolation of DNA; 7. Functional assays of nuclei; Analysis of DNA-binding proteins; Transcription assays; References 4. Subcellular fractionation of mitochondria1. Introduction; 2. Purification of mitochondria from various eukaryotic sources; Introduction; Protocols for purification of mitochondria from several eukaryotic sources; Further purification of mitochondrial fractions; 3. Determination of mitochondrial purity; Introduction; Use of the oxygen electrode to determine mitochondrial integrity; Determination of the integrity of the mitochondrial outer membrane; Glucose hexokinase trap method for estimation of P:O ratios; 4. Subfractionation of mitochondria Preparation of submitochondrial particles by sonication |
| Record Nr. | UNINA-9910455468303321 |
| Oxford ; ; New York, : IRL Press at Oxford University Press, c1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Subcellular fractionation [[electronic resource] ] : a practical approach / / edited by J.M. Graham and D. Rickwood
| Subcellular fractionation [[electronic resource] ] : a practical approach / / edited by J.M. Graham and D. Rickwood |
| Pubbl/distr/stampa | Oxford ; ; New York, : IRL Press at Oxford University Press, c1997 |
| Descrizione fisica | 1 online resource (360 p.) |
| Disciplina | 571.6/5 |
| Altri autori (Persone) |
GrahamJ. M <1943-> (John M.)
RickwoodD (David) |
| Collana | The practical approach series |
| Soggetto topico | Subcellular fractionation |
| ISBN |
1-283-66474-7
0-19-159161-0 0-585-48392-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Contents; List of contributors; Abbreviations; 1. Homogenization of tissues and cells; 1. Introduction; 2. Aims of the homogenization procedure; 3. Influence of sample type; 4. Homogenization media; 5. Methods of homogenization; Type 1 homogenizers; Type 2 homogenizers; 6. Homogenization of tissues and cells; Mammalian liver; Brain; Muscle; Mammalian tissue culture cells; Plant organelles; Yeast; Other fungi and algae; Trypanosomes; Bacteria; References; 2. Isolation of subcellular fractions; 1. Introduction; 2. Composition of a tissue homogenate; 3. Properties of cell organelles
Factors affecting organelle density and size4. Centrifugal methods for the separation of organelles; Separation by size; Separation by density; Density perturbation; 5. Non-centrifugal procedures; Immunoisolation; Separation by electrophoresis; 6. Identification of separated material; Marker enzymes; Introduced markers for endocytic and exocytic pathways; Characteristic non-enzymatic proteins; 7. Assessment of the purity of fractions; Purity and purification; Problems from cell heterogeneity within tissues; Problems arising from organelle fragmentation Missorting in the exocytic and endocytic pathways8. Fractionation problems; No separation; Aggregation following resuspension of a fraction; Poor recovery of markers; Damage to cell structures; 9. A systematic approach to cell fractionation; Preliminary studies; Determination of the properties of components of the homogenate; Method development; Simplification of the separation; References; 3. Isolation and characterization of nuclei and nuclear subfractions; 1. Introduction; 2. Methods of preparing purified nuclei; Types of cells and tissue samples; Homogenization media Homogenization methodsCentrifugation conditions; Assays of nuclear purity; 3. Methods for purifying metaphase chromosomes; 4. Isolation of nuclear subfractions; Preparation of nucleoli; Preparation of nuclear membranes; Isolation of nuclear matrix; Preparation of nucleoids; 5. Isolation of nucleoprotein complexes; Isolation of polynucleosomes of chromatin; Ribonucleoproteins; 6. Isolation of nuclear macromolecules; Isolation of nuclear proteins; Isolation of nuclear RNA; Isolation of DNA; 7. Functional assays of nuclei; Analysis of DNA-binding proteins; Transcription assays; References 4. Subcellular fractionation of mitochondria1. Introduction; 2. Purification of mitochondria from various eukaryotic sources; Introduction; Protocols for purification of mitochondria from several eukaryotic sources; Further purification of mitochondrial fractions; 3. Determination of mitochondrial purity; Introduction; Use of the oxygen electrode to determine mitochondrial integrity; Determination of the integrity of the mitochondrial outer membrane; Glucose hexokinase trap method for estimation of P:O ratios; 4. Subfractionation of mitochondria Preparation of submitochondrial particles by sonication |
| Record Nr. | UNINA-9910780114603321 |
| Oxford ; ; New York, : IRL Press at Oxford University Press, c1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
