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Molecular biology techniques [[electronic resource] ] : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow
| Molecular biology techniques [[electronic resource] ] : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow |
| Autore | Carson Sue, Dr. |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 |
| Descrizione fisica | 1 online resource (xxvi, 200 pages) : color illustrations |
| Disciplina |
572.8/078
572.8078 |
| Altri autori (Persone) |
MillerHeather Bennett
WitherowD. Scott (Dennis Scott) |
| Soggetto topico |
Molecular biology
Molecular biology - Technique |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-283-31097-X
9786613310972 0-12-385545-4 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. |
| Record Nr. | UNINA-9910457771203321 |
Carson Sue, Dr.
|
||
| Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology techniques [[electronic resource] ] : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow
| Molecular biology techniques [[electronic resource] ] : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow |
| Autore | Carson Sue, Dr. |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 |
| Descrizione fisica | 1 online resource (xxvi, 200 pages) : color illustrations |
| Disciplina |
572.8/078
572.8078 |
| Altri autori (Persone) |
MillerHeather B
WitherowD. Scott (Dennis Scott) |
| Soggetto topico |
Molecular biology
Molecular biology - Technique |
| ISBN |
1-283-31097-X
9786613310972 0-12-385545-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. |
| Record Nr. | UNINA-9910781937603321 |
|
Carson Sue, Dr.
|
||
| Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology techniques : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow
| Molecular biology techniques : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow |
| Autore | Carson Sue, Dr. |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 |
| Descrizione fisica | 1 online resource (xxvi, 200 pages) : color illustrations |
| Disciplina |
572.8/078
572.8078 |
| Altri autori (Persone) |
MillerHeather B
WitherowD. Scott |
| Soggetto topico |
Molecular biology
Molecular biology - Technique |
| ISBN |
9786613310972
9781283310970 128331097X 9780123855459 0123855454 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. |
| Record Nr. | UNINA-9910811007703321 |
|
Carson Sue, Dr.
|
||
| Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
A wavelet tour of signal processing [[electronic resource] ] : the Sparse way / / Stephane Mallat
| A wavelet tour of signal processing [[electronic resource] ] : the Sparse way / / Stephane Mallat |
| Autore | Mallat S. G (Stéphane G.) |
| Edizione | [Sparse ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Elsevier /Academic Press, c2009 |
| Descrizione fisica | 1 online resource (829 p.) |
| Disciplina |
621.382/2015152433
621.3822015152433 |
| Soggetto topico |
Signal processing - Mathematics
Wavelets (Mathematics) |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-98216-4
9786611982164 0-08-092202-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; A Wavelet Tour of Signal Processing; Copyright Page; Dedication Page; Table of Contents; Preface to the Sparse Edition; Notations; Chapter 1. Sparse Representations; 1.1 Computational Harmonic Analysis; 1.1.1 The Fourier Kingdom; 1.1.2 Wavelet Bases; 1.2 Approximation and Processing in Bases; 1.2.1 Sampling with Linear Approximations; 1.2.2 Sparse Nonlinear Approximations; 1.2.3 Compression; 1.2.4 Denoising; 1.3 Time-Frequency Dictionaries; 1.3.1 Heisenberg Uncertainty; 1.3.2 Windowed Fourier Transform; 1.3.3 Continuous Wavelet Transform; 1.3.4 Time-Frequency Orthonormal Bases
1.4 Sparsity in Redundant Dictionaries1.4.1 Frame Analysis and Synthesis; 1.4.2 Ideal Dictionary Approximations; 1.4.3 Pursuit in Dictionaries; 1.5 Inverse Problems; 1.5.1 Diagonal Inverse Estimation; 1.5.2 Super-resolution and Compressive Sensing; 1.6 Travel Guide; 1.6.1 Reproducible Computational Science; 1.6.2 Book Road Map; Chapter 2. The Fourier Kingdom; 2.1 Linear Time-Invariant Filtering; 2.1.1 Impulse Response; 2.1.2 Transfer Functions; 2.2 Fourier Integrals; 2.2.1 Fourier Transform in L1(R); 2.2.2 Fourier Transform in L2(R); 2.2.3 Examples; 2.3 Properties; 2.3.1 Regularity and Decay 2.3.2 Uncertainty Principle2.3.3 Total Variation; 2.4 Two-Dimensional Fourier Transform; 2.5 Exercises; Chapter 3. Discrete Revolution; 3.1 Sampling Analog Signals; 3.1.1 Shannon-Whittaker Sampling Theorem; 3.1.2 Aliasing; 3.1.3 General Sampling and Linear Analog Conversions; 3.2 Discrete Time-Invariant Filters; 3.2.1 Impulse Response and Transfer Function; 3.2.2 Fourier Series; 3.3 Finite Signals; 3.3.1 Circular Convolutions; 3.3.2 Discrete Fourier Transform; 3.3.3 Fast Fourier Transform; 3.3.4 Fast Convolutions; 3.4 Discrete Image Processing; 3.4.1 Two-Dimensional Sampling Theorems 3.4.2 Discrete Image Filtering3.4.3 Circular Convolutions and Fourier Basis; 3.5 Exercises; Chapter 4. Time Meets Frequency; 4.1 Time-Frequency Atoms; 4.2 Windowed Fourier Transform; 4.2.1 Completeness and Stability; 4.2.2 Choice of Window; 4.2.3 Discrete Windowed Fourier Transform; 4.3 Wavelet Transforms; 4.3.1 Real Wavelets; 4.3.2 Analytic Wavelets; 4.3.3 Discrete Wavelets; 4.4 Time-Frequency Geometry of Instantaneous Frequencies; 4.4.1 Analytic Instantaneous Frequency; 4.4.2 Windowed Fourier Ridges; 4.4.3 Wavelet Ridges; 4.5 Quadratic Time-Frequency Energy; 4.5.1 Wigner-Ville Distribution 4.5.2 Interferences and Positivity4.5.3 Cohen's Class; 4.5.4 Discrete Wigner-Ville Computations; 4.6 Exercises; Chapter 5. Frames; 5.1 Frames and Riesz Bases; 5.1.1 Stable Analysis and Synthesis Operators; 5.1.2 Dual Frame and Pseudo Inverse; 5.1.3 Dual-Frame Analysis and Synthesis Computations; 5.1.4 Frame Projector and Reproducing Kernel; 5.1.5 Translation-Invariant Frames; 5.2 Translation-Invariant Dyadic Wavelet Transform; 5.2.1 Dyadic Wavelet Design; 5.2.2 Algorithme à Trous; 5.3 Subsampled Wavelet Frames; 5.4 Windowed Fourier Frames; 5.4.1 Tight Frames; 5.4.2 General Frames 5.5 Multiscale Directional Frames For Images |
| Record Nr. | UNINA-9910454413803321 |
|
Mallat S. G (Stéphane G.)
|
||
| Amsterdam ; ; Boston, : Elsevier /Academic Press, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
A wavelet tour of signal processing [[electronic resource] ] : the Sparse way / / Stephane Mallat
| A wavelet tour of signal processing [[electronic resource] ] : the Sparse way / / Stephane Mallat |
| Autore | Mallat S. G (Stéphane G.) |
| Edizione | [Sparse ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Elsevier /Academic Press, c2009 |
| Descrizione fisica | 1 online resource (829 p.) |
| Disciplina |
621.382/2015152433
621.3822015152433 |
| Soggetto topico |
Signal processing - Mathematics
Wavelets (Mathematics) |
| ISBN |
1-281-98216-4
9786611982164 0-08-092202-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; A Wavelet Tour of Signal Processing; Copyright Page; Dedication Page; Table of Contents; Preface to the Sparse Edition; Notations; Chapter 1. Sparse Representations; 1.1 Computational Harmonic Analysis; 1.1.1 The Fourier Kingdom; 1.1.2 Wavelet Bases; 1.2 Approximation and Processing in Bases; 1.2.1 Sampling with Linear Approximations; 1.2.2 Sparse Nonlinear Approximations; 1.2.3 Compression; 1.2.4 Denoising; 1.3 Time-Frequency Dictionaries; 1.3.1 Heisenberg Uncertainty; 1.3.2 Windowed Fourier Transform; 1.3.3 Continuous Wavelet Transform; 1.3.4 Time-Frequency Orthonormal Bases
1.4 Sparsity in Redundant Dictionaries1.4.1 Frame Analysis and Synthesis; 1.4.2 Ideal Dictionary Approximations; 1.4.3 Pursuit in Dictionaries; 1.5 Inverse Problems; 1.5.1 Diagonal Inverse Estimation; 1.5.2 Super-resolution and Compressive Sensing; 1.6 Travel Guide; 1.6.1 Reproducible Computational Science; 1.6.2 Book Road Map; Chapter 2. The Fourier Kingdom; 2.1 Linear Time-Invariant Filtering; 2.1.1 Impulse Response; 2.1.2 Transfer Functions; 2.2 Fourier Integrals; 2.2.1 Fourier Transform in L1(R); 2.2.2 Fourier Transform in L2(R); 2.2.3 Examples; 2.3 Properties; 2.3.1 Regularity and Decay 2.3.2 Uncertainty Principle2.3.3 Total Variation; 2.4 Two-Dimensional Fourier Transform; 2.5 Exercises; Chapter 3. Discrete Revolution; 3.1 Sampling Analog Signals; 3.1.1 Shannon-Whittaker Sampling Theorem; 3.1.2 Aliasing; 3.1.3 General Sampling and Linear Analog Conversions; 3.2 Discrete Time-Invariant Filters; 3.2.1 Impulse Response and Transfer Function; 3.2.2 Fourier Series; 3.3 Finite Signals; 3.3.1 Circular Convolutions; 3.3.2 Discrete Fourier Transform; 3.3.3 Fast Fourier Transform; 3.3.4 Fast Convolutions; 3.4 Discrete Image Processing; 3.4.1 Two-Dimensional Sampling Theorems 3.4.2 Discrete Image Filtering3.4.3 Circular Convolutions and Fourier Basis; 3.5 Exercises; Chapter 4. Time Meets Frequency; 4.1 Time-Frequency Atoms; 4.2 Windowed Fourier Transform; 4.2.1 Completeness and Stability; 4.2.2 Choice of Window; 4.2.3 Discrete Windowed Fourier Transform; 4.3 Wavelet Transforms; 4.3.1 Real Wavelets; 4.3.2 Analytic Wavelets; 4.3.3 Discrete Wavelets; 4.4 Time-Frequency Geometry of Instantaneous Frequencies; 4.4.1 Analytic Instantaneous Frequency; 4.4.2 Windowed Fourier Ridges; 4.4.3 Wavelet Ridges; 4.5 Quadratic Time-Frequency Energy; 4.5.1 Wigner-Ville Distribution 4.5.2 Interferences and Positivity4.5.3 Cohen's Class; 4.5.4 Discrete Wigner-Ville Computations; 4.6 Exercises; Chapter 5. Frames; 5.1 Frames and Riesz Bases; 5.1.1 Stable Analysis and Synthesis Operators; 5.1.2 Dual Frame and Pseudo Inverse; 5.1.3 Dual-Frame Analysis and Synthesis Computations; 5.1.4 Frame Projector and Reproducing Kernel; 5.1.5 Translation-Invariant Frames; 5.2 Translation-Invariant Dyadic Wavelet Transform; 5.2.1 Dyadic Wavelet Design; 5.2.2 Algorithme à Trous; 5.3 Subsampled Wavelet Frames; 5.4 Windowed Fourier Frames; 5.4.1 Tight Frames; 5.4.2 General Frames 5.5 Multiscale Directional Frames For Images |
| Record Nr. | UNINA-9910782686003321 |
|
Mallat S. G (Stéphane G.)
|
||
| Amsterdam ; ; Boston, : Elsevier /Academic Press, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
A wavelet tour of signal processing : the Sparse way / / Stephane Mallat
| A wavelet tour of signal processing : the Sparse way / / Stephane Mallat |
| Autore | Mallat S. G (Stéphane G.) |
| Edizione | [Sparse ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Elsevier /Academic Press, c2009 |
| Descrizione fisica | 1 online resource (829 p.) |
| Disciplina |
621.382/2015152433
621.3822015152433 |
| Soggetto topico |
Signal processing - Mathematics
Wavelets (Mathematics) |
| ISBN |
9786611982164
9781281982162 1281982164 9780080922027 0080922023 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; A Wavelet Tour of Signal Processing; Copyright Page; Dedication Page; Table of Contents; Preface to the Sparse Edition; Notations; Chapter 1. Sparse Representations; 1.1 Computational Harmonic Analysis; 1.1.1 The Fourier Kingdom; 1.1.2 Wavelet Bases; 1.2 Approximation and Processing in Bases; 1.2.1 Sampling with Linear Approximations; 1.2.2 Sparse Nonlinear Approximations; 1.2.3 Compression; 1.2.4 Denoising; 1.3 Time-Frequency Dictionaries; 1.3.1 Heisenberg Uncertainty; 1.3.2 Windowed Fourier Transform; 1.3.3 Continuous Wavelet Transform; 1.3.4 Time-Frequency Orthonormal Bases
1.4 Sparsity in Redundant Dictionaries1.4.1 Frame Analysis and Synthesis; 1.4.2 Ideal Dictionary Approximations; 1.4.3 Pursuit in Dictionaries; 1.5 Inverse Problems; 1.5.1 Diagonal Inverse Estimation; 1.5.2 Super-resolution and Compressive Sensing; 1.6 Travel Guide; 1.6.1 Reproducible Computational Science; 1.6.2 Book Road Map; Chapter 2. The Fourier Kingdom; 2.1 Linear Time-Invariant Filtering; 2.1.1 Impulse Response; 2.1.2 Transfer Functions; 2.2 Fourier Integrals; 2.2.1 Fourier Transform in L1(R); 2.2.2 Fourier Transform in L2(R); 2.2.3 Examples; 2.3 Properties; 2.3.1 Regularity and Decay 2.3.2 Uncertainty Principle2.3.3 Total Variation; 2.4 Two-Dimensional Fourier Transform; 2.5 Exercises; Chapter 3. Discrete Revolution; 3.1 Sampling Analog Signals; 3.1.1 Shannon-Whittaker Sampling Theorem; 3.1.2 Aliasing; 3.1.3 General Sampling and Linear Analog Conversions; 3.2 Discrete Time-Invariant Filters; 3.2.1 Impulse Response and Transfer Function; 3.2.2 Fourier Series; 3.3 Finite Signals; 3.3.1 Circular Convolutions; 3.3.2 Discrete Fourier Transform; 3.3.3 Fast Fourier Transform; 3.3.4 Fast Convolutions; 3.4 Discrete Image Processing; 3.4.1 Two-Dimensional Sampling Theorems 3.4.2 Discrete Image Filtering3.4.3 Circular Convolutions and Fourier Basis; 3.5 Exercises; Chapter 4. Time Meets Frequency; 4.1 Time-Frequency Atoms; 4.2 Windowed Fourier Transform; 4.2.1 Completeness and Stability; 4.2.2 Choice of Window; 4.2.3 Discrete Windowed Fourier Transform; 4.3 Wavelet Transforms; 4.3.1 Real Wavelets; 4.3.2 Analytic Wavelets; 4.3.3 Discrete Wavelets; 4.4 Time-Frequency Geometry of Instantaneous Frequencies; 4.4.1 Analytic Instantaneous Frequency; 4.4.2 Windowed Fourier Ridges; 4.4.3 Wavelet Ridges; 4.5 Quadratic Time-Frequency Energy; 4.5.1 Wigner-Ville Distribution 4.5.2 Interferences and Positivity4.5.3 Cohen's Class; 4.5.4 Discrete Wigner-Ville Computations; 4.6 Exercises; Chapter 5. Frames; 5.1 Frames and Riesz Bases; 5.1.1 Stable Analysis and Synthesis Operators; 5.1.2 Dual Frame and Pseudo Inverse; 5.1.3 Dual-Frame Analysis and Synthesis Computations; 5.1.4 Frame Projector and Reproducing Kernel; 5.1.5 Translation-Invariant Frames; 5.2 Translation-Invariant Dyadic Wavelet Transform; 5.2.1 Dyadic Wavelet Design; 5.2.2 Algorithme à Trous; 5.3 Subsampled Wavelet Frames; 5.4 Windowed Fourier Frames; 5.4.1 Tight Frames; 5.4.2 General Frames 5.5 Multiscale Directional Frames For Images |
| Record Nr. | UNINA-9910824696403321 |
|
Mallat S. G (Stéphane G.)
|
||
| Amsterdam ; ; Boston, : Elsevier /Academic Press, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
