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Aptamers in bioanalysis [[electronic resource] /] / edited by Marco Mascini
| Aptamers in bioanalysis [[electronic resource] /] / edited by Marco Mascini |
| Pubbl/distr/stampa | Hoboken, NJ, : Wiley, c2009 |
| Descrizione fisica | 1 online resource (346 p.) |
| Disciplina | 572.85 |
| Altri autori (Persone) | MasciniMarco |
| Soggetto topico |
Oligonucleotides
Biochemistry |
| ISBN |
1-282-11267-8
9786612112676 0-470-38077-2 0-470-38076-4 |
| Classificazione |
CHE 808f
CHE 860f WC 4150 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
APTAMERS IN BIOANALYSIS; CONTENTS; PREFACE; CONTRIBUTORS; I INTRODUCTION; 1 APTAMERS: LIGANDS FOR ALL REASONS; 1.1 Introduction; 1.2 The Power of Selection and Aptamer Refinement; 1.3 The Chemistry Drives the Shape; 1.4 Aptaregulators; 1.5 Aptasensors; 1.6 Prospects; References; 2 SELEX AND ITS RECENT OPTIMIZATIONS; 2.1 Introduction; 2.2 Aptamers and Their Selection by SELEX; 2.3 Modifications of SELEX Technology; 2.4 Advantages and Limitations of Aptamers and Their Selection Technology; 2.5 Applications of Aptamers Being Developed for the Market; 2.6 Future Perspectives; References
II BIOSENSORS3 ELECTROCHEMICAL APTASENSORS; 3.1 Introduction; 3.2 Electrochemical Aptasensor Based on Redox-Active Aptamer Monolayers Linked to Electrodes; 3.3 Enzyme-Based Amplified Electrochemical Aptasensors; 3.4 Amplified Electrochemical Aptasensors Based on Nanoparticles; 3.5 Label-Free Electrochemical Aptasensors; 3.6 Field-Effect Transistor-Based Aptasensors; 3.7 Conclusions and Perspectives; References; 4 APTAMERS: HYBRIDS BETWEEN NATURE AND TECHNOLOGY; 4.1 Introduction; 4.2 Specific Features of Aptamers; 4.3 Electrochemical Detection of Nucleic Acids 4.4 Cytochrome c Binding by Aptamers4.5 DNA Machines and Aptamers; References; 5 DETECTION OF PROTEIN-APTAMER INTERACTIONS BY MEANS OF ELECTROCHEMICAL INDICATORS AND TRANSVERSE SHEAR MODE METHOD; 5.1 Introduction; 5.2 Immobilization of Aptamers on a Solid Support; 5.3 Detection of Aptamer-Ligand Interactions; 5.3.1 Electrochemical Methods; 5.3.2 Acoustic Methods; 5.4 Conclusions; References; 6 BIOSENSORS USING THE APTAMERIC ENZYME SUBUNIT: THE USE OF APTAMERS IN THE ALLOSTERIC CONTROL OF ENZYMES; 6.1 Aptamers as Molecular Recognition Elements of Biosensors 6.1.1 Comparing Aptamers to Antibodies6.1.2 Signaling Aptamers; 6.2 Homogeneous Sensing; 6.2.1 Biosensor Systems That Do Not Require Bound-Free Separation; 6.2.2 Aptameric Enzyme Subunit; 6.3 Evolution-mimicking Algorithm for the Improvement of Aptamers; References; 7 NANOMATERIAL-BASED LABEL-FREE APTASENSORS; 7.1 Introduction; 7.2 Label-Free Electrochemical Aptasensors; 7.3 Field-Effect Transistor-Based Aptasensors; 7.4 Label-Free Aptasensors Based on Localized Surface Plasmon Resonance; 7.5 Forthcoming Challenges and Concluding Remarks; References 8 APTAMER-BASED BIOANALYTICAL ASSAYS: AMPLIFICATION STRATEGIES8.1 Introduction; 8.2 Bioanalytical Assays Based on Aptamer-Functionalized Nanoparticles; 8.3 Aptamers and Quantum Dot-Based Assays; 8.4 Aptazymes and Aptamer-Based Machines; 8.5 Polymerase Chain Reaction as an Amplification Method in Aptamer-Based Assays; 8.6 Conclusions; References; III APPLICATIONS; 9 KINETIC CAPILLARY ELECTROPHORESIS FOR SELECTION, CHARACTERIZATION, AND ANALYTICAL UTILIZATION OF APTAMERS; 9.1 Introduction; 9.1.1 Kinetic Capillary Electrophoresis; 9.1.2 The Concept of NECEEM and ECEEM 9.2 Selection of Aptamers Using KCE Methods for Partitioning and Affinity Control |
| Record Nr. | UNINA-9910146399103321 |
| Hoboken, NJ, : Wiley, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Aptamers in bioanalysis / / edited by Marco Mascini
| Aptamers in bioanalysis / / edited by Marco Mascini |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Hoboken, NJ, : Wiley, c2009 |
| Descrizione fisica | 1 online resource (346 p.) |
| Disciplina | 572.85 |
| Altri autori (Persone) | MasciniMarco |
| Soggetto topico |
Oligonucleotides
Biochemistry |
| ISBN |
1-282-11267-8
9786612112676 0-470-38077-2 0-470-38076-4 |
| Classificazione |
CHE 808f
CHE 860f WC 4150 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
APTAMERS IN BIOANALYSIS; CONTENTS; PREFACE; CONTRIBUTORS; I INTRODUCTION; 1 APTAMERS: LIGANDS FOR ALL REASONS; 1.1 Introduction; 1.2 The Power of Selection and Aptamer Refinement; 1.3 The Chemistry Drives the Shape; 1.4 Aptaregulators; 1.5 Aptasensors; 1.6 Prospects; References; 2 SELEX AND ITS RECENT OPTIMIZATIONS; 2.1 Introduction; 2.2 Aptamers and Their Selection by SELEX; 2.3 Modifications of SELEX Technology; 2.4 Advantages and Limitations of Aptamers and Their Selection Technology; 2.5 Applications of Aptamers Being Developed for the Market; 2.6 Future Perspectives; References
II BIOSENSORS3 ELECTROCHEMICAL APTASENSORS; 3.1 Introduction; 3.2 Electrochemical Aptasensor Based on Redox-Active Aptamer Monolayers Linked to Electrodes; 3.3 Enzyme-Based Amplified Electrochemical Aptasensors; 3.4 Amplified Electrochemical Aptasensors Based on Nanoparticles; 3.5 Label-Free Electrochemical Aptasensors; 3.6 Field-Effect Transistor-Based Aptasensors; 3.7 Conclusions and Perspectives; References; 4 APTAMERS: HYBRIDS BETWEEN NATURE AND TECHNOLOGY; 4.1 Introduction; 4.2 Specific Features of Aptamers; 4.3 Electrochemical Detection of Nucleic Acids 4.4 Cytochrome c Binding by Aptamers4.5 DNA Machines and Aptamers; References; 5 DETECTION OF PROTEIN-APTAMER INTERACTIONS BY MEANS OF ELECTROCHEMICAL INDICATORS AND TRANSVERSE SHEAR MODE METHOD; 5.1 Introduction; 5.2 Immobilization of Aptamers on a Solid Support; 5.3 Detection of Aptamer-Ligand Interactions; 5.3.1 Electrochemical Methods; 5.3.2 Acoustic Methods; 5.4 Conclusions; References; 6 BIOSENSORS USING THE APTAMERIC ENZYME SUBUNIT: THE USE OF APTAMERS IN THE ALLOSTERIC CONTROL OF ENZYMES; 6.1 Aptamers as Molecular Recognition Elements of Biosensors 6.1.1 Comparing Aptamers to Antibodies6.1.2 Signaling Aptamers; 6.2 Homogeneous Sensing; 6.2.1 Biosensor Systems That Do Not Require Bound-Free Separation; 6.2.2 Aptameric Enzyme Subunit; 6.3 Evolution-mimicking Algorithm for the Improvement of Aptamers; References; 7 NANOMATERIAL-BASED LABEL-FREE APTASENSORS; 7.1 Introduction; 7.2 Label-Free Electrochemical Aptasensors; 7.3 Field-Effect Transistor-Based Aptasensors; 7.4 Label-Free Aptasensors Based on Localized Surface Plasmon Resonance; 7.5 Forthcoming Challenges and Concluding Remarks; References 8 APTAMER-BASED BIOANALYTICAL ASSAYS: AMPLIFICATION STRATEGIES8.1 Introduction; 8.2 Bioanalytical Assays Based on Aptamer-Functionalized Nanoparticles; 8.3 Aptamers and Quantum Dot-Based Assays; 8.4 Aptazymes and Aptamer-Based Machines; 8.5 Polymerase Chain Reaction as an Amplification Method in Aptamer-Based Assays; 8.6 Conclusions; References; III APPLICATIONS; 9 KINETIC CAPILLARY ELECTROPHORESIS FOR SELECTION, CHARACTERIZATION, AND ANALYTICAL UTILIZATION OF APTAMERS; 9.1 Introduction; 9.1.1 Kinetic Capillary Electrophoresis; 9.1.2 The Concept of NECEEM and ECEEM 9.2 Selection of Aptamers Using KCE Methods for Partitioning and Affinity Control |
| Record Nr. | UNINA-9910825105103321 |
| Hoboken, NJ, : Wiley, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Chemical cytometry : ultrasensitive analysis of single cells
| Chemical cytometry : ultrasensitive analysis of single cells |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2010 |
| Disciplina | 572 |
| Soggetto topico |
Cytochemistry
Histological Techniques Biochemistry Investigative Techniques Cytological Techniques Histology Anatomy Analytical, Diagnostic and Therapeutic Techniques and Equipment Biological Science Disciplines Chemistry Clinical Laboratory Techniques Natural Science Disciplines Disciplines and Occupations Methods Histocytochemistry Biology Health & Biological Sciences Cytology |
| ISBN | 3-527-62964-5 |
| Classificazione |
WC 4150
WC 5100 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Origin, current status, and future perspectives of chemical cytometry / Norman J. Dovichi -- Metabolic cytometry - the study of glycosphingolipid metabolism in single primary cells of the dorsal root ganglia / Colin D. Whitmore ... [et al.] -- Cell signaling studied at the single-cell level / Angela Proctor ... [et al.] -- Ultrasensitive detection of low-copy-number molecules from single cells / Kangning Ren and Hongkai Wu -- Capillary electrophoresis of nucleic acids at the single cell level / Ni Li and Wenwan Zhong -- Microfluidic technology for single-cell analysis / Yan Chen and Jiang F. Zhong -- On-chip electroporation and electrofusion for single-cell engineering / Ana Valero and Albert van den Berg -- Electroporative flow cytometry for single-cell analysis / Chang Lu ... [et al.] -- Ultrasensitive analysis of individual cells via droplet microfluidics / Robert M. Lorenz and Daniel T. Chiu -- Probing exocytosis at single cells using electrochemistry / Yan Dong ... [et al.] -- Electrochemical determination of enzyme activity in single cells / Wenrui Jin -- Single-cell mass spectrometry / Ann Knolhoff, Stanislaw Rubakhin, and Jonathan V. Sweedler -- Optical sensing arrays for single-cell analysis / Ragnhild D. Whitaker and David R. Walt. |
| Record Nr. | UNINA-9910139426603321 |
| [Place of publication not identified], : Wiley VCH, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Chemical cytometry : ultrasensitive analysis of single cells
| Chemical cytometry : ultrasensitive analysis of single cells |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2010 |
| Disciplina | 572 |
| Soggetto topico |
Cytochemistry
Histological Techniques Biochemistry Investigative Techniques Cytological Techniques Histology Anatomy Analytical, Diagnostic and Therapeutic Techniques and Equipment Biological Science Disciplines Chemistry Clinical Laboratory Techniques Natural Science Disciplines Disciplines and Occupations Methods Histocytochemistry Biology Health & Biological Sciences Cytology |
| ISBN | 3-527-62964-5 |
| Classificazione |
WC 4150
WC 5100 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Origin, current status, and future perspectives of chemical cytometry / Norman J. Dovichi -- Metabolic cytometry - the study of glycosphingolipid metabolism in single primary cells of the dorsal root ganglia / Colin D. Whitmore ... [et al.] -- Cell signaling studied at the single-cell level / Angela Proctor ... [et al.] -- Ultrasensitive detection of low-copy-number molecules from single cells / Kangning Ren and Hongkai Wu -- Capillary electrophoresis of nucleic acids at the single cell level / Ni Li and Wenwan Zhong -- Microfluidic technology for single-cell analysis / Yan Chen and Jiang F. Zhong -- On-chip electroporation and electrofusion for single-cell engineering / Ana Valero and Albert van den Berg -- Electroporative flow cytometry for single-cell analysis / Chang Lu ... [et al.] -- Ultrasensitive analysis of individual cells via droplet microfluidics / Robert M. Lorenz and Daniel T. Chiu -- Probing exocytosis at single cells using electrochemistry / Yan Dong ... [et al.] -- Electrochemical determination of enzyme activity in single cells / Wenrui Jin -- Single-cell mass spectrometry / Ann Knolhoff, Stanislaw Rubakhin, and Jonathan V. Sweedler -- Optical sensing arrays for single-cell analysis / Ragnhild D. Whitaker and David R. Walt. |
| Record Nr. | UNINA-9910830214303321 |
| [Place of publication not identified], : Wiley VCH, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Chemical cytometry : ultrasensitive analysis of single cells
| Chemical cytometry : ultrasensitive analysis of single cells |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2010 |
| Disciplina | 572 |
| Soggetto topico |
Cytochemistry
Histological Techniques Biochemistry Investigative Techniques Cytological Techniques Histology Anatomy Analytical, Diagnostic and Therapeutic Techniques and Equipment Biological Science Disciplines Chemistry Clinical Laboratory Techniques Natural Science Disciplines Disciplines and Occupations Methods Histocytochemistry Biology Health & Biological Sciences Cytology |
| ISBN | 3-527-62964-5 |
| Classificazione |
WC 4150
WC 5100 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Origin, current status, and future perspectives of chemical cytometry / Norman J. Dovichi -- Metabolic cytometry - the study of glycosphingolipid metabolism in single primary cells of the dorsal root ganglia / Colin D. Whitmore ... [et al.] -- Cell signaling studied at the single-cell level / Angela Proctor ... [et al.] -- Ultrasensitive detection of low-copy-number molecules from single cells / Kangning Ren and Hongkai Wu -- Capillary electrophoresis of nucleic acids at the single cell level / Ni Li and Wenwan Zhong -- Microfluidic technology for single-cell analysis / Yan Chen and Jiang F. Zhong -- On-chip electroporation and electrofusion for single-cell engineering / Ana Valero and Albert van den Berg -- Electroporative flow cytometry for single-cell analysis / Chang Lu ... [et al.] -- Ultrasensitive analysis of individual cells via droplet microfluidics / Robert M. Lorenz and Daniel T. Chiu -- Probing exocytosis at single cells using electrochemistry / Yan Dong ... [et al.] -- Electrochemical determination of enzyme activity in single cells / Wenrui Jin -- Single-cell mass spectrometry / Ann Knolhoff, Stanislaw Rubakhin, and Jonathan V. Sweedler -- Optical sensing arrays for single-cell analysis / Ragnhild D. Whitaker and David R. Walt. |
| Record Nr. | UNINA-9910876726103321 |
| [Place of publication not identified], : Wiley VCH, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||