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Gene biotechnology / / William Wu. [et al.]
| Gene biotechnology / / William Wu. [et al.] |
| Autore | Wu William |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | Boca Raton : , : CRC Press, , 2011 |
| Descrizione fisica | 1 online resource (562 p.) |
| Disciplina | 660.6/5078 |
| Soggetto topico |
Genetic engineering
Molecular biology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
0-429-16573-0
1-4398-4832-7 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Contents; Preface; Authors; Chapter 1: Strategies for Novel Research Projects and/or Research Grant Funding; Chapter 2: Rapid Isolation of Specific cDNAs or Genes by PCR; Chapter 3: Construction and Screening of Subtracted and Complete Expression cDNA Libraries; Chapter 4: Subcloning of Genes or DNA Fragments; Chapter 5: Nonisotopic and Isotopic DNA or RNA Sequencing; Chapter 6: Bioinformation Superhighway and Computer Databases of Nucleic Acids and Proteins; Chapter 7: Characterization of DNA or Genes by Southern Blot Hybridization
Chapter 8: Gene Overexpression by Sense RNA in Mammalian SystemsChapter 9: Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies; Chapter 10: Analysis of Gene Expression at Functional Genomic Level Using Northern Blotting or PCR; Chapter 11: Analysis of Gene Expression at Proteomic Level via Western Blotting; Chapter 12: Analysis of Cellular DNA or Abundance of mRNA by Radioactivity In Situ Hybridization; Chapter 13: Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization Chapter 14: In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAsChapter 15: Isolation and Characterization of Genes from Genomic DNA Libraries; Chapter 16: Mouse Stem Cells as a Model Mammalian Cell Line for Gene Expression; Chapter 17: Strategies for Gene Double Knockout; Chapter 18: Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells; Chapter 19: Quantitative Analysis of Functional Genome by Real-Time RT-PCR; Chapter 20: High-Throughput Analysis of Gene Expression by Cutting-Edge Technology-DNA Microarrays (Gene Chips) Chapter 21: Construction and Screening of Human Antibody Libraries Using Phage Display TechnologyChapter 22: Down-Regulation of Gene Expression in Mammalian Systems via siRNA Technology; Chapter 23: Strategies for Gene Cloning, Expression, and Identification of Protein-Protein Interaction; Chapter 24: Conditional Gene Knockout; Chapter 25: How to Write a Research Manuscript for Publication in an English Journal; Chapter 26: How to Protect Your Discovery and Invention : Patent 101; Chapter 27: Determination of Transgene Copy Numbers and Practical Biocalculation; Back Cover |
| Record Nr. | UNINA-9910461354103321 |
Wu William
|
||
| Boca Raton : , : CRC Press, , 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Gene biotechnology / / William Wu. [et al.]
| Gene biotechnology / / William Wu. [et al.] |
| Autore | Wu William |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | Boca Raton : , : CRC Press, , 2011 |
| Descrizione fisica | 1 online resource (562 p.) |
| Disciplina | 660.6/5078 |
| Soggetto topico |
Genetic engineering
Molecular biology |
| ISBN |
0-429-16573-0
1-4398-4832-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Contents; Preface; Authors; Chapter 1: Strategies for Novel Research Projects and/or Research Grant Funding; Chapter 2: Rapid Isolation of Specific cDNAs or Genes by PCR; Chapter 3: Construction and Screening of Subtracted and Complete Expression cDNA Libraries; Chapter 4: Subcloning of Genes or DNA Fragments; Chapter 5: Nonisotopic and Isotopic DNA or RNA Sequencing; Chapter 6: Bioinformation Superhighway and Computer Databases of Nucleic Acids and Proteins; Chapter 7: Characterization of DNA or Genes by Southern Blot Hybridization
Chapter 8: Gene Overexpression by Sense RNA in Mammalian SystemsChapter 9: Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies; Chapter 10: Analysis of Gene Expression at Functional Genomic Level Using Northern Blotting or PCR; Chapter 11: Analysis of Gene Expression at Proteomic Level via Western Blotting; Chapter 12: Analysis of Cellular DNA or Abundance of mRNA by Radioactivity In Situ Hybridization; Chapter 13: Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization Chapter 14: In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAsChapter 15: Isolation and Characterization of Genes from Genomic DNA Libraries; Chapter 16: Mouse Stem Cells as a Model Mammalian Cell Line for Gene Expression; Chapter 17: Strategies for Gene Double Knockout; Chapter 18: Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells; Chapter 19: Quantitative Analysis of Functional Genome by Real-Time RT-PCR; Chapter 20: High-Throughput Analysis of Gene Expression by Cutting-Edge Technology-DNA Microarrays (Gene Chips) Chapter 21: Construction and Screening of Human Antibody Libraries Using Phage Display TechnologyChapter 22: Down-Regulation of Gene Expression in Mammalian Systems via siRNA Technology; Chapter 23: Strategies for Gene Cloning, Expression, and Identification of Protein-Protein Interaction; Chapter 24: Conditional Gene Knockout; Chapter 25: How to Write a Research Manuscript for Publication in an English Journal; Chapter 26: How to Protect Your Discovery and Invention : Patent 101; Chapter 27: Determination of Transgene Copy Numbers and Practical Biocalculation; Back Cover |
| Record Nr. | UNINA-9910789436103321 |
|
Wu William
|
||
| Boca Raton : , : CRC Press, , 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Gene biotechnology / / William Wu. [et al.]
| Gene biotechnology / / William Wu. [et al.] |
| Autore | Wu William |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | Boca Raton, : Taylor & Francis, 2011 |
| Descrizione fisica | 1 online resource (562 p.) |
| Disciplina | 660.6/5078 |
| Altri autori (Persone) | WuWilliam |
| Soggetto topico |
Genetic engineering
Molecular biology |
| ISBN |
1-04-022192-0
0-429-16573-0 1-4398-4832-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Contents; Preface; Authors; Chapter 1: Strategies for Novel Research Projects and/or Research Grant Funding; Chapter 2: Rapid Isolation of Specific cDNAs or Genes by PCR; Chapter 3: Construction and Screening of Subtracted and Complete Expression cDNA Libraries; Chapter 4: Subcloning of Genes or DNA Fragments; Chapter 5: Nonisotopic and Isotopic DNA or RNA Sequencing; Chapter 6: Bioinformation Superhighway and Computer Databases of Nucleic Acids and Proteins; Chapter 7: Characterization of DNA or Genes by Southern Blot Hybridization
Chapter 8: Gene Overexpression by Sense RNA in Mammalian SystemsChapter 9: Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies; Chapter 10: Analysis of Gene Expression at Functional Genomic Level Using Northern Blotting or PCR; Chapter 11: Analysis of Gene Expression at Proteomic Level via Western Blotting; Chapter 12: Analysis of Cellular DNA or Abundance of mRNA by Radioactivity In Situ Hybridization; Chapter 13: Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization Chapter 14: In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAsChapter 15: Isolation and Characterization of Genes from Genomic DNA Libraries; Chapter 16: Mouse Stem Cells as a Model Mammalian Cell Line for Gene Expression; Chapter 17: Strategies for Gene Double Knockout; Chapter 18: Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells; Chapter 19: Quantitative Analysis of Functional Genome by Real-Time RT-PCR; Chapter 20: High-Throughput Analysis of Gene Expression by Cutting-Edge Technology-DNA Microarrays (Gene Chips) Chapter 21: Construction and Screening of Human Antibody Libraries Using Phage Display TechnologyChapter 22: Down-Regulation of Gene Expression in Mammalian Systems via siRNA Technology; Chapter 23: Strategies for Gene Cloning, Expression, and Identification of Protein-Protein Interaction; Chapter 24: Conditional Gene Knockout; Chapter 25: How to Write a Research Manuscript for Publication in an English Journal; Chapter 26: How to Protect Your Discovery and Invention : Patent 101; Chapter 27: Determination of Transgene Copy Numbers and Practical Biocalculation; Back Cover |
| Record Nr. | UNINA-9910970759303321 |
|
Wu William
|
||
| Boca Raton, : Taylor & Francis, 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||