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Directed molecular evolution of proteins : or how to improve enzymes for biocatalysis
| Directed molecular evolution of proteins : or how to improve enzymes for biocatalysis |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Descrizione fisica | 1 online resource (359 pages) |
| Disciplina | 660.6/34 |
| Soggetto topico |
Enzymes - Biotechnology
Biocatalysis - Evolution Proteins - Chemical modification Proteins Molecular evolution Combinatorial chemistry Directed Molecular Evolution Protein Engineering Genetic Engineering Genetic Techniques Investigative Techniques Analytical, Diagnostic and Therapeutic Techniques and Equipment Biomedical Engineering Health & Biological Sciences |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-55837-7
9786610558377 3-527-60064-7 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910146244503321 |
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Directed molecular evolution of proteins : or how to improve enzymes for biocatalysis
| Directed molecular evolution of proteins : or how to improve enzymes for biocatalysis |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Descrizione fisica | 1 online resource (359 pages) |
| Disciplina | 660.6/34 |
| Soggetto topico |
Enzymes - Biotechnology
Biocatalysis - Evolution Proteins - Chemical modification Proteins Molecular evolution Combinatorial chemistry Directed Molecular Evolution Protein Engineering Genetic Engineering Genetic Techniques Investigative Techniques Analytical, Diagnostic and Therapeutic Techniques and Equipment Biomedical Engineering Health & Biological Sciences |
| ISBN |
1-280-55837-7
9786610558377 3-527-60064-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910830354403321 |
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Directed molecular evolution of proteins : or how to improve enzymes for biocatalysis
| Directed molecular evolution of proteins : or how to improve enzymes for biocatalysis |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Descrizione fisica | 1 online resource (359 pages) |
| Disciplina | 660.6/34 |
| Soggetto topico |
Enzymes - Biotechnology
Biocatalysis - Evolution Proteins - Chemical modification Proteins Molecular evolution Combinatorial chemistry Directed Molecular Evolution Protein Engineering Genetic Engineering Genetic Techniques Investigative Techniques Analytical, Diagnostic and Therapeutic Techniques and Equipment Biomedical Engineering Health & Biological Sciences |
| ISBN |
1-280-55837-7
9786610558377 3-527-60064-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910840878503321 |
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Enzyme catalysis in organic synthesis : a comprehensive handbook
| Enzyme catalysis in organic synthesis : a comprehensive handbook |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Disciplina | 660.6/34 |
| Soggetto topico |
Enzymes - Biotechnology
Organic compounds - Synthesis Enzymes - Industrial applications Catalysis Chemistry, Organic Enzymes Physicochemical Processes Chemical Processes Chemistry Enzymes and Coenzymes Natural Science Disciplines Physicochemical Phenomena Chemicals and Drugs Chemical Phenomena Phenomena and Processes Disciplines and Occupations Biomedical Engineering Health & Biological Sciences |
| ISBN | 3-527-61826-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISA-996197429503316 |
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Enzyme technologies [[electronic resource] ] : metagenomics, evolution, biocatalysis, and biosynthesis / / edited by Wu-Kuang Yeh, Hsiu-Chiung Yang, James R. McCarthy
| Enzyme technologies [[electronic resource] ] : metagenomics, evolution, biocatalysis, and biosynthesis / / edited by Wu-Kuang Yeh, Hsiu-Chiung Yang, James R. McCarthy |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, c2010 |
| Descrizione fisica | 1 online resource (390 p.) |
| Disciplina | 660.6/34 |
| Altri autori (Persone) |
YehWu-Kuang <1942->
YangHsiu-Chiung McCarthyJames R. <1943-> |
| Collana | Chemical biology of enzymes for biotechnology and pharmaceutical applications |
| Soggetto topico |
Enzymes - Biotechnology
Pharmaceutical biotechnology |
| ISBN |
1-283-30028-1
9786613300287 0-470-62729-8 0-470-62730-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
ENZYME TECHNOLOGIES; CONTENTS; Contributors; Preface; PART A NEW APPROACHES TO FINDING AND MODIFYING ENZYMES; 1 Functional Metagenomics as a Technique for the Discovery of Novel Enzymes and Natural Products; 2 Directed Enzyme and Pathway Evolution; 3 Combining Natural Biodiversity and Molecular-Directed Evolution to Develop New Industrial Biocatalysts and Drugs; 4 Principles of Enzyme Optimization for the Rapid Creation of Industrial Biocatalysts; PART B BIOCATALYTIC APPLICATIONS; 5 Enzyme Catalysis in the Synthesis of Active Pharmaceutical Ingredients
6 Enzymatic Processes for the Production of Pharmaceutical Intermediates7 Novel Developments Employing Redox Enzymes: Old Enzymes in New Clothes; PART C BIOSYNTHETIC APPLICATIONS; 8 Drug Discovery and Development by Combinatorial Biosynthesis; 9 Reprogramming Daptomycin and A54145 Biosynthesis to Produce Novel Lipopeptide Antibiotics; 10 Pathway and Enzyme Engineering and Applications for Glycodiversification; Index |
| Record Nr. | UNINA-9910132377603321 |
| Hoboken, N.J., : Wiley, c2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Enzyme technologies [[electronic resource] ] : metagenomics, evolution, biocatalysis, and biosynthesis / / edited by Wu-Kuang Yeh, Hsiu-Chiung Yang, James R. McCarthy
| Enzyme technologies [[electronic resource] ] : metagenomics, evolution, biocatalysis, and biosynthesis / / edited by Wu-Kuang Yeh, Hsiu-Chiung Yang, James R. McCarthy |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, c2010 |
| Descrizione fisica | 1 online resource (390 p.) |
| Disciplina | 660.6/34 |
| Altri autori (Persone) |
YehWu-Kuang <1942->
YangHsiu-Chiung McCarthyJames R. <1943-> |
| Collana | Chemical biology of enzymes for biotechnology and pharmaceutical applications |
| Soggetto topico |
Enzymes - Biotechnology
Pharmaceutical biotechnology |
| ISBN |
1-283-30028-1
9786613300287 0-470-62729-8 0-470-62730-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
ENZYME TECHNOLOGIES; CONTENTS; Contributors; Preface; PART A NEW APPROACHES TO FINDING AND MODIFYING ENZYMES; 1 Functional Metagenomics as a Technique for the Discovery of Novel Enzymes and Natural Products; 2 Directed Enzyme and Pathway Evolution; 3 Combining Natural Biodiversity and Molecular-Directed Evolution to Develop New Industrial Biocatalysts and Drugs; 4 Principles of Enzyme Optimization for the Rapid Creation of Industrial Biocatalysts; PART B BIOCATALYTIC APPLICATIONS; 5 Enzyme Catalysis in the Synthesis of Active Pharmaceutical Ingredients
6 Enzymatic Processes for the Production of Pharmaceutical Intermediates7 Novel Developments Employing Redox Enzymes: Old Enzymes in New Clothes; PART C BIOSYNTHETIC APPLICATIONS; 8 Drug Discovery and Development by Combinatorial Biosynthesis; 9 Reprogramming Daptomycin and A54145 Biosynthesis to Produce Novel Lipopeptide Antibiotics; 10 Pathway and Enzyme Engineering and Applications for Glycodiversification; Index |
| Record Nr. | UNINA-9910823246203321 |
| Hoboken, N.J., : Wiley, c2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Enzymes in industry : production and applications / / edited by Wolfgang Aehle
| Enzymes in industry : production and applications / / edited by Wolfgang Aehle |
| Edizione | [Third, completely revised edition.] |
| Pubbl/distr/stampa | Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2007 |
| Descrizione fisica | 1 online resource (518 p.) |
| Disciplina |
660.6/34
660.634 |
| Soggetto topico |
Enzymes - Biotechnology
Enzymes - Industrial applications |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-31178-2
9786611311780 3-527-61709-4 3-527-61710-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Enzymes in Industry; Contents; Preface to the Third Edition; List of Contributors; Abbreviations; 1 Introduction; 1.1 History; 1.2 Enzyme Nomenclature; 1.2.1 General Principles of Nomenclature; 1.2.2 Classification and Numbering of Enzymes; 1.3 Structure of Enzymes; 1.3.1 Primary Structure; 1.3.2 Three-Dimensional Structure; 1.3.3 Quaternary Structure, Folding, and Domains; 1.3.4 The Ribozyme; 1.4 Enzymes and Molecular Biology; 1.4.1 Biosynthesis of Enzymes; 1.4.2 Enzymes and DNA; 2 Catalytic Activity of Enzymes [57, 58, 60]; 2.1 Factors Governing Catalytic Activity [63]; 2.1.1 Temperature
2.1.2 Value of pH2.1.3 Activation; 2.1.4 Inhibition [65]; 2.1.5 Allostery [66]; 2.1.6 Biogenic Regulation of Activity; 2.2 Enzyme Assays; 2.2.1 Reaction Rate as a Measure of Catalytic Activity; 2.2.2 Definition of Units; 2.2.3 Absorption Photometry [71]; 2.2.4 Fluorometry [78]; 2.2.5 Luminometry [80]; 2.2.6 Radiometry; 2.2.7 Potentiometry [81]; 2.2.8 Conductometry; 2.2.9 Calorimetry; 2.2.10 Polarimetry; 2.2.11 Manometry; 2.2.12 Viscosimetry; 2.2.13 Turbidimetry; 2.2.14 Immobilized Enzymes [91]; 2.2.15 Electrophoresis; 2.3 Quality Evaluation of Enzyme Preparations; 2.3.1 Quality Criteria 2.3.2 Specific Activity2.3.3 Protein Determination; 2.3.4 Contaminating Activities; 2.3.5 Electrophoretic Purity; 2.3.6 High-Performance Liquid Chromatography [111]; 2.3.7 Performance Test; 2.3.8 Amino Acid Analysis and Protein Sequence Analysis; 2.3.9 Stability [93]; 2.3.10 Formulation of Enzyme Preparations; 3 General Production Methods; 3.1 Microbial Production; 3.1.1 Organism and Enzyme Synthesis; 3.1.2 Strain Improvement; 3.1.3 Physiological Optimization; 3.1.4 The Fermentor and its Limitations; 3.1.5 Process Design; 3.1.6 Modeling and Optimization; 3.1.7 Instrumentation and Control 3.2 Isolation and Purification [172-187]3.2.1 Preparation of Biological Starting Materials; 3.2.1.1 Cell Disruption by Mechanical Methods; 3.2.1.2 Cell Disruption by Nonmechanical Methods; 3.2.2. Separation of Solid Matter; 3.2.2.1 Filtration; 3.2.2.2 Centrifugation; 3.2.2.3 Extraction; 3.2.2.4 Flocculation and Flotation; 3.2.3 Concentration; 3.2.3.1 Thermal Methods; 3.2.3.2 Precipitation; 3.2.3.3 Ultrafiltration; 3.2.4 Purification; 3.2.4.1 Crystallization; 3.2.4.2 Electrophoresis; 3.2.4.3 Chromatography; 3.2.5 Product Formulation; 3.2.6 Waste Disposal; 3.3 Immobilization; 3.3.1 Definitions 3.3.2 History3.3.3 Methods; 3.3.3.1 Carrier Binding; 3.3.3.2 Cross-linking; 3.3.3.3 Entrapment; 3.3.4 Characterization; 3.3.5 Application; 4 Discovery and Development of Enzymes; 4.1 Enzyme Screening; 4.1.1 Overview; 4.1.2 Natural Isolate Screening; 4.1.3 Molecular Screening; 4.1.4 Environmental Gene Screening; 4.1.5 Genomic Screening; 4.1.6 Proteomic Screening; 4.2 Protein Engineering; 4.2.1 Introduction; 4.2.2 Application of Protein Engineering in Academia and Industry; 4.2.3 Outlook; 5 Industrial Enzymes; 5.1 Enzymes in Food Applications; 5.1.1 Enzymes in Baking; 5.1.1.1 Introduction 5.1.1.2 Amylases |
| Record Nr. | UNINA-9910144278703321 |
| Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Enzymes in industry : production and applications / / edited by Wolfgang Aehle
| Enzymes in industry : production and applications / / edited by Wolfgang Aehle |
| Edizione | [Third, completely revised edition.] |
| Pubbl/distr/stampa | Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2007 |
| Descrizione fisica | 1 online resource (518 p.) |
| Disciplina |
660.6/34
660.634 |
| Soggetto topico |
Enzymes - Biotechnology
Enzymes - Industrial applications |
| ISBN |
1-281-31178-2
9786611311780 3-527-61709-4 3-527-61710-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Enzymes in Industry; Contents; Preface to the Third Edition; List of Contributors; Abbreviations; 1 Introduction; 1.1 History; 1.2 Enzyme Nomenclature; 1.2.1 General Principles of Nomenclature; 1.2.2 Classification and Numbering of Enzymes; 1.3 Structure of Enzymes; 1.3.1 Primary Structure; 1.3.2 Three-Dimensional Structure; 1.3.3 Quaternary Structure, Folding, and Domains; 1.3.4 The Ribozyme; 1.4 Enzymes and Molecular Biology; 1.4.1 Biosynthesis of Enzymes; 1.4.2 Enzymes and DNA; 2 Catalytic Activity of Enzymes [57, 58, 60]; 2.1 Factors Governing Catalytic Activity [63]; 2.1.1 Temperature
2.1.2 Value of pH2.1.3 Activation; 2.1.4 Inhibition [65]; 2.1.5 Allostery [66]; 2.1.6 Biogenic Regulation of Activity; 2.2 Enzyme Assays; 2.2.1 Reaction Rate as a Measure of Catalytic Activity; 2.2.2 Definition of Units; 2.2.3 Absorption Photometry [71]; 2.2.4 Fluorometry [78]; 2.2.5 Luminometry [80]; 2.2.6 Radiometry; 2.2.7 Potentiometry [81]; 2.2.8 Conductometry; 2.2.9 Calorimetry; 2.2.10 Polarimetry; 2.2.11 Manometry; 2.2.12 Viscosimetry; 2.2.13 Turbidimetry; 2.2.14 Immobilized Enzymes [91]; 2.2.15 Electrophoresis; 2.3 Quality Evaluation of Enzyme Preparations; 2.3.1 Quality Criteria 2.3.2 Specific Activity2.3.3 Protein Determination; 2.3.4 Contaminating Activities; 2.3.5 Electrophoretic Purity; 2.3.6 High-Performance Liquid Chromatography [111]; 2.3.7 Performance Test; 2.3.8 Amino Acid Analysis and Protein Sequence Analysis; 2.3.9 Stability [93]; 2.3.10 Formulation of Enzyme Preparations; 3 General Production Methods; 3.1 Microbial Production; 3.1.1 Organism and Enzyme Synthesis; 3.1.2 Strain Improvement; 3.1.3 Physiological Optimization; 3.1.4 The Fermentor and its Limitations; 3.1.5 Process Design; 3.1.6 Modeling and Optimization; 3.1.7 Instrumentation and Control 3.2 Isolation and Purification [172-187]3.2.1 Preparation of Biological Starting Materials; 3.2.1.1 Cell Disruption by Mechanical Methods; 3.2.1.2 Cell Disruption by Nonmechanical Methods; 3.2.2. Separation of Solid Matter; 3.2.2.1 Filtration; 3.2.2.2 Centrifugation; 3.2.2.3 Extraction; 3.2.2.4 Flocculation and Flotation; 3.2.3 Concentration; 3.2.3.1 Thermal Methods; 3.2.3.2 Precipitation; 3.2.3.3 Ultrafiltration; 3.2.4 Purification; 3.2.4.1 Crystallization; 3.2.4.2 Electrophoresis; 3.2.4.3 Chromatography; 3.2.5 Product Formulation; 3.2.6 Waste Disposal; 3.3 Immobilization; 3.3.1 Definitions 3.3.2 History3.3.3 Methods; 3.3.3.1 Carrier Binding; 3.3.3.2 Cross-linking; 3.3.3.3 Entrapment; 3.3.4 Characterization; 3.3.5 Application; 4 Discovery and Development of Enzymes; 4.1 Enzyme Screening; 4.1.1 Overview; 4.1.2 Natural Isolate Screening; 4.1.3 Molecular Screening; 4.1.4 Environmental Gene Screening; 4.1.5 Genomic Screening; 4.1.6 Proteomic Screening; 4.2 Protein Engineering; 4.2.1 Introduction; 4.2.2 Application of Protein Engineering in Academia and Industry; 4.2.3 Outlook; 5 Industrial Enzymes; 5.1 Enzymes in Food Applications; 5.1.1 Enzymes in Baking; 5.1.1.1 Introduction 5.1.1.2 Amylases |
| Record Nr. | UNINA-9910830115203321 |
| Weinheim, [Germany] : , : Wiley-VCH Verlag GmbH & Co. KGaA, , 2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Perspectives on lipase enzyme technology [[electronic resource] /] / J. Geraldine Sandana Mala and Satoru Takeuchi
| Perspectives on lipase enzyme technology [[electronic resource] /] / J. Geraldine Sandana Mala and Satoru Takeuchi |
| Autore | Mala J. Geraldine Sandana |
| Pubbl/distr/stampa | New York, : Nova Science Publishers, Inc., c2009 |
| Descrizione fisica | 1 online resource (161 p.) |
| Disciplina | 660.6/34 |
| Altri autori (Persone) | TakeuchiSatoru |
| Collana | Biotechnology in agriculture, industry and medicine series |
| Soggetto topico | Lipase - Biotechnology |
| Soggetto genere / forma | Electronic books. |
| ISBN | 1-61728-560-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Biotechnology : the science and origin of lipase -- Isolation strategies of microbial lipases -- Lipase production : defining the media -- Lipase purification and characterization -- Lipase structure -- Molecular cloning and overexpression -- Bioinformatics -- Lipases : biocatalysts for the future. |
| Record Nr. | UNINA-9910460052803321 |
Mala J. Geraldine Sandana
|
||
| New York, : Nova Science Publishers, Inc., c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Perspectives on lipase enzyme technology [[electronic resource] /] / J. Geraldine Sandana Mala and Satoru Takeuchi
| Perspectives on lipase enzyme technology [[electronic resource] /] / J. Geraldine Sandana Mala and Satoru Takeuchi |
| Autore | Mala J. Geraldine Sandana |
| Pubbl/distr/stampa | New York, : Nova Science Publishers, Inc., c2009 |
| Descrizione fisica | 1 online resource (161 p.) |
| Disciplina | 660.6/34 |
| Altri autori (Persone) | TakeuchiSatoru |
| Collana | Biotechnology in agriculture, industry and medicine series |
| Soggetto topico | Lipase - Biotechnology |
| ISBN | 1-61728-560-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Biotechnology : the science and origin of lipase -- Isolation strategies of microbial lipases -- Lipase production : defining the media -- Lipase purification and characterization -- Lipase structure -- Molecular cloning and overexpression -- Bioinformatics -- Lipases : biocatalysts for the future. |
| Record Nr. | UNINA-9910785290703321 |
|
Mala J. Geraldine Sandana
|
||
| New York, : Nova Science Publishers, Inc., c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
