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Methods in animal proteomics / / editors, P. David Eckersall, Phillip D. Whitfield
| Methods in animal proteomics / / editors, P. David Eckersall, Phillip D. Whitfield |
| Pubbl/distr/stampa | Chichester, West Sussex ; ; Ames, Iowa, : Wiley-Blackwell, 2011 |
| Descrizione fisica | 1 online resource (910 p.) |
| Disciplina |
636.089
636.0892398 |
| Altri autori (Persone) |
EckersallP. D
WhitfieldPhillip D |
| Soggetto topico |
Proteomics - Methodology
Veterinary medicine - Methodology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
0-470-96064-7
0-470-96063-9 0-470-96066-3 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Title page; Copyright page; Contributors; Acknowledgments; Section 1: Exploring Animal Proteomes; 1 An Introduction to Animal Proteomics; 1.1 Proteomics and Animal Systems; 1.2 Exploring Animal Proteomes; 1.3 Applications of Proteomics in Animal Systems; 2 Types of Sample and Experimental Planning; 2.1 Introduction; 2.2 Types of Samples and Their Collection; 2.3 General Preparation of Body Fluids, Cultured Cells in Suspension, and Blood; 2.4 Homogenization, Clarification, and Simplification; 2.5 Determination of Protein Concentration in Proteomic Samples; 2.6 Enzymatic Digestion
2.7 Experimental Design: Strategies for Simplification and Enrichment2.8 Specialized Techniques for Sample Preparation; 2.9 Experimental Planning and Design: General Guidelines; 2.10 Biomarker Discovery: Special Considerations for Experimental Planning; 2.11 Concluding Remarks; 3 Protein Separation Strategies; 3.1 Introduction; 3.2 "Classical" 2DE; 3.3 Other Varieties of 2DE; 3.4 Sample Complexity; 3.5 The Influence of Staining; 3.6 "Post-detection"; 3.7 Future Trends; 4 Methods and Approaches to Mass Spectroscopy-Based Protein Identification; 4.1 Introduction; 4.2 MS 4.3 Sample Preparation4.4 Protein Identification; 4.5 Linking Mass Spectra with Proteins; 4.6 Validation of MS Data; 4.7 Conclusions; 5 Bioinformatics in Animal Proteomics; 5.1 Introduction; 5.2 Methods; 5.3 Future Trends; 5.4 Sources of Further Information; 6 Comparative Proteomic Approaches; 6.1 Global Study of Proteins; 6.2 Approaches to Characterize the Proteome; 6.3 Quantitative Proteomics; 6.4 Bioinformatics and Biostatistics; 6.5 Future Prospects; 7 Advancing Technologies for Spatial and Temporal Proteomics; 7.1 Introduction; 7.2 Investigating the Spatial Proteome 7.3 Temporal Proteomics7.4 Case Study: Spatial and Temporal Profiling in the Chicken; 7.5 Combining Spatial and Temporal Proteomics; 7.6 Recent Developments in Animal Proteomics; 7.7 Future Trends; 7.8 Conclusions; 7.9 Sources of Further Information; Section 2: Applications of Proteomics in Animal Biology; 8 Proteomic Strategies to Investigate Adaptive Processes; 8.1 Introduction; 8.2 Hibernation Physiology and Behavior; 8.3 Sampling Strategy; 8.4 Sample Collection and Storage; 8.5 Design and Execution of a Quantitative Proteomic Experiment; 8.6 Conclusion 9 Investigation of Animal Venoms and Toxins9.1 Introduction; 9.2 Natural Bioresources; 9.3 Pharmaceutical Success Stories; 9.4 The Methods in Toxin Sequencing; 9.5 Reducing Complexity by Chromatography; 9.6 Bioassay Considerations; 9.7 Classical Edman; 9.8 Overview of MS Methods for Chemical Prospecting; 9.9 Soft Ionization Methods; 9.10 Common Mass Analyzers for Peptide Analysis; 9.11 Information Obtained from Different MS Systems; 9.12 Cloning Studies; 9.13 New Developments and Future Trends; 9.14 Conclusions; 9.15 Other Sources of Information; 10 Proteomics in Animal Health and Disease 10.1 Introduction |
| Record Nr. | UNINA-9910133839703321 |
| Chichester, West Sussex ; ; Ames, Iowa, : Wiley-Blackwell, 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Methods in animal proteomics / / editors, P. David Eckersall, Phillip D. Whitfield
| Methods in animal proteomics / / editors, P. David Eckersall, Phillip D. Whitfield |
| Pubbl/distr/stampa | Chichester, West Sussex ; ; Ames, Iowa, : Wiley-Blackwell, 2011 |
| Descrizione fisica | 1 online resource (910 p.) |
| Disciplina |
636.089
636.0892398 |
| Altri autori (Persone) |
EckersallP. D
WhitfieldPhillip D |
| Soggetto topico |
Proteomics - Methodology
Veterinary medicine - Methodology |
| ISBN |
0-470-96064-7
0-470-96063-9 0-470-96066-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover; Title page; Copyright page; Contributors; Acknowledgments; Section 1: Exploring Animal Proteomes; 1 An Introduction to Animal Proteomics; 1.1 Proteomics and Animal Systems; 1.2 Exploring Animal Proteomes; 1.3 Applications of Proteomics in Animal Systems; 2 Types of Sample and Experimental Planning; 2.1 Introduction; 2.2 Types of Samples and Their Collection; 2.3 General Preparation of Body Fluids, Cultured Cells in Suspension, and Blood; 2.4 Homogenization, Clarification, and Simplification; 2.5 Determination of Protein Concentration in Proteomic Samples; 2.6 Enzymatic Digestion
2.7 Experimental Design: Strategies for Simplification and Enrichment2.8 Specialized Techniques for Sample Preparation; 2.9 Experimental Planning and Design: General Guidelines; 2.10 Biomarker Discovery: Special Considerations for Experimental Planning; 2.11 Concluding Remarks; 3 Protein Separation Strategies; 3.1 Introduction; 3.2 "Classical" 2DE; 3.3 Other Varieties of 2DE; 3.4 Sample Complexity; 3.5 The Influence of Staining; 3.6 "Post-detection"; 3.7 Future Trends; 4 Methods and Approaches to Mass Spectroscopy-Based Protein Identification; 4.1 Introduction; 4.2 MS 4.3 Sample Preparation4.4 Protein Identification; 4.5 Linking Mass Spectra with Proteins; 4.6 Validation of MS Data; 4.7 Conclusions; 5 Bioinformatics in Animal Proteomics; 5.1 Introduction; 5.2 Methods; 5.3 Future Trends; 5.4 Sources of Further Information; 6 Comparative Proteomic Approaches; 6.1 Global Study of Proteins; 6.2 Approaches to Characterize the Proteome; 6.3 Quantitative Proteomics; 6.4 Bioinformatics and Biostatistics; 6.5 Future Prospects; 7 Advancing Technologies for Spatial and Temporal Proteomics; 7.1 Introduction; 7.2 Investigating the Spatial Proteome 7.3 Temporal Proteomics7.4 Case Study: Spatial and Temporal Profiling in the Chicken; 7.5 Combining Spatial and Temporal Proteomics; 7.6 Recent Developments in Animal Proteomics; 7.7 Future Trends; 7.8 Conclusions; 7.9 Sources of Further Information; Section 2: Applications of Proteomics in Animal Biology; 8 Proteomic Strategies to Investigate Adaptive Processes; 8.1 Introduction; 8.2 Hibernation Physiology and Behavior; 8.3 Sampling Strategy; 8.4 Sample Collection and Storage; 8.5 Design and Execution of a Quantitative Proteomic Experiment; 8.6 Conclusion 9 Investigation of Animal Venoms and Toxins9.1 Introduction; 9.2 Natural Bioresources; 9.3 Pharmaceutical Success Stories; 9.4 The Methods in Toxin Sequencing; 9.5 Reducing Complexity by Chromatography; 9.6 Bioassay Considerations; 9.7 Classical Edman; 9.8 Overview of MS Methods for Chemical Prospecting; 9.9 Soft Ionization Methods; 9.10 Common Mass Analyzers for Peptide Analysis; 9.11 Information Obtained from Different MS Systems; 9.12 Cloning Studies; 9.13 New Developments and Future Trends; 9.14 Conclusions; 9.15 Other Sources of Information; 10 Proteomics in Animal Health and Disease 10.1 Introduction |
| Record Nr. | UNINA-9910830213203321 |
| Chichester, West Sussex ; ; Ames, Iowa, : Wiley-Blackwell, 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||