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Fundamental medical mycology [[electronic resource] /] / Errol Reiss, H. Jean Shadomy, G. Marshall Lyon III
| Fundamental medical mycology [[electronic resource] /] / Errol Reiss, H. Jean Shadomy, G. Marshall Lyon III |
| Autore | Reiss Errol |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Blackwell, c2012 |
| Descrizione fisica | 1 online resource (655 p.) |
| Disciplina | 616.9/6901 |
| Altri autori (Persone) |
ShadomyH. Jean
LyonG. Marshall |
| Soggetto topico |
Medical mycology
Microbiology |
| ISBN |
1-283-28271-2
9786613282712 1-118-10176-6 1-118-10177-4 1-118-10175-8 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Fundamental Medical Mycology; Fundamental Medical Mycology; Contents; Contents; Preface; Acknowledgments; Part One Introduction to Fundamental Medical Mycology, Laboratory Diagnostic Methods, and Antifungal Therapy; 1. Introduction to Fundamental Medical Mycology; 1.1 Topics not Covered, or Receiving Secondary Emphasis; 1.2 Biosafety Considerations: Before You Begin Work with Pathogenic Fungi...; 1.2.1 Biological Safety Cabinets (BSC); 1.2.2 Precautions to Take in Handling Etiologic Agents that Cause Systemic Mycoses; 1.2.3 Additional Precautions at Biosafety Level 3 (BSL 3)
1.2.4 Safety Training1.2.5 Disinfectants and Waste Disposal; 1.3 Fungi Defined: Their Ecologic Niche; 1.4 Medical Mycology; 1.5 A Brief History of Medical Mycology; 1.5.1 Ancient Greece; 1.5.2 Middle Ages; 1.5.3 Twentieth Century; 1.5.4 Endemic Mycoses in the Americas; 1.5.5 Era of Immunosuppression in the Treatment of Cancer, Maintenance of Organ Transplants, and Autoimmune Diseases; 1.5.6 Opportunistic Mycoses; 1.5.7 HIV/AIDS; 1.5.8 Twenty-first Century; 1.6 Rationale for Fungal Identification; 1.6.1 Developing the Treatment Plan; 1.6.2 Investigating Outbreaks 1.6.3 Determining the Susceptibility to Antifungal Agents1.6.4 Estimating the Significance of Fungi Generally Considered to be Opportunists or Saprobes; 1.6.5 Types of Vegetative Growth; 1.7 Sporulation; 1.8 Dimorphism; 1.8.1 Dimorphism and Pathogenesis; 1.9 Sex in Fungi; 1.9.1 Anamorph and Teleomorph Nomenclature; 1.10 Classification of Mycoses Based on the Primary Site of Pathology; 1.10.1 Superficial Mycoses; 1.10.2 Cutaneous Mycoses; 1.10.3 Systemic Opportunistic Mycoses; 1.10.4 Subcutaneous Mycoses; 1.10.5 Endemic Mycoses Caused by Dimorphic Environmental Molds 1.11 Taxonomy/Classification: Kingdom Fungi1.11.1 The Phylogenetic Species Concept for Classification; 1.11.2 The Higher Level Classification of Kingdom Fungi; 1.12 General Composition of the Fungal Cell; 1.12.1 Yeast Cell Cycle; 1.12.2 Hyphal Morphogenesis; 1.12.3 Cell Wall; 1.13 Primary Pathogens; 1.13.1 Susceptibility to Primary Pathogens; 1.14 Endemic Versus Worldwide Presence; 1.15 Opportunistic Fungal Pathogens; 1.15.1 Susceptibility to Opportunistic Fungal Pathogens: Host Factors; 1.16 Determinants of Pathogenicity; General References in Medical Mycology Selected References for Introduction to Fundamental Medical MycologyWebsites Cited; Questions; 2. Laboratory Diagnostic Methods in Medical Mycology; 2.1 Who Is Responsible for Identifying Pathogenic Fungi?; 2.1.1 Role of the Clinical Laboratorian; 2.1.2 Role of the Physician; 2.2 What Methods are Used to Identify Pathogenic Fungi?; 2.2.1 Culture and Identification; 2.3 Laboratory Detection, Recovery, and Identification of Fungi in the Clinical Microbiology Laboratory; 2.3.1 The Laboratory Manual; 2.3.2 Specimen Collection; 2.3.3 Direct Examination; 2.3.4 Histopathology; 2.3.5 Culture 2.3.6 Storage and Cryopreservation of Cultures for QA and QC in the Clinical Mycology Laboratory |
| Record Nr. | UNINA-9910141246603321 |
Reiss Errol
|
||
| Hoboken, N.J., : Wiley-Blackwell, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Fundamental medical mycology / / Errol Reiss, H. Jean Shadomy, G. Marshall Lyon III
| Fundamental medical mycology / / Errol Reiss, H. Jean Shadomy, G. Marshall Lyon III |
| Autore | Reiss Errol |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Blackwell, c2012 |
| Descrizione fisica | 1 online resource (655 p.) |
| Disciplina | 616.9/6901 |
| Altri autori (Persone) |
ShadomyH. Jean
LyonG. Marshall |
| Soggetto topico |
Medical mycology
Microbiology |
| ISBN |
1-283-28271-2
9786613282712 1-118-10176-6 1-118-10177-4 1-118-10175-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Fundamental Medical Mycology; Fundamental Medical Mycology; Contents; Contents; Preface; Acknowledgments; Part One Introduction to Fundamental Medical Mycology, Laboratory Diagnostic Methods, and Antifungal Therapy; 1. Introduction to Fundamental Medical Mycology; 1.1 Topics not Covered, or Receiving Secondary Emphasis; 1.2 Biosafety Considerations: Before You Begin Work with Pathogenic Fungi...; 1.2.1 Biological Safety Cabinets (BSC); 1.2.2 Precautions to Take in Handling Etiologic Agents that Cause Systemic Mycoses; 1.2.3 Additional Precautions at Biosafety Level 3 (BSL 3)
1.2.4 Safety Training1.2.5 Disinfectants and Waste Disposal; 1.3 Fungi Defined: Their Ecologic Niche; 1.4 Medical Mycology; 1.5 A Brief History of Medical Mycology; 1.5.1 Ancient Greece; 1.5.2 Middle Ages; 1.5.3 Twentieth Century; 1.5.4 Endemic Mycoses in the Americas; 1.5.5 Era of Immunosuppression in the Treatment of Cancer, Maintenance of Organ Transplants, and Autoimmune Diseases; 1.5.6 Opportunistic Mycoses; 1.5.7 HIV/AIDS; 1.5.8 Twenty-first Century; 1.6 Rationale for Fungal Identification; 1.6.1 Developing the Treatment Plan; 1.6.2 Investigating Outbreaks 1.6.3 Determining the Susceptibility to Antifungal Agents1.6.4 Estimating the Significance of Fungi Generally Considered to be Opportunists or Saprobes; 1.6.5 Types of Vegetative Growth; 1.7 Sporulation; 1.8 Dimorphism; 1.8.1 Dimorphism and Pathogenesis; 1.9 Sex in Fungi; 1.9.1 Anamorph and Teleomorph Nomenclature; 1.10 Classification of Mycoses Based on the Primary Site of Pathology; 1.10.1 Superficial Mycoses; 1.10.2 Cutaneous Mycoses; 1.10.3 Systemic Opportunistic Mycoses; 1.10.4 Subcutaneous Mycoses; 1.10.5 Endemic Mycoses Caused by Dimorphic Environmental Molds 1.11 Taxonomy/Classification: Kingdom Fungi1.11.1 The Phylogenetic Species Concept for Classification; 1.11.2 The Higher Level Classification of Kingdom Fungi; 1.12 General Composition of the Fungal Cell; 1.12.1 Yeast Cell Cycle; 1.12.2 Hyphal Morphogenesis; 1.12.3 Cell Wall; 1.13 Primary Pathogens; 1.13.1 Susceptibility to Primary Pathogens; 1.14 Endemic Versus Worldwide Presence; 1.15 Opportunistic Fungal Pathogens; 1.15.1 Susceptibility to Opportunistic Fungal Pathogens: Host Factors; 1.16 Determinants of Pathogenicity; General References in Medical Mycology Selected References for Introduction to Fundamental Medical MycologyWebsites Cited; Questions; 2. Laboratory Diagnostic Methods in Medical Mycology; 2.1 Who Is Responsible for Identifying Pathogenic Fungi?; 2.1.1 Role of the Clinical Laboratorian; 2.1.2 Role of the Physician; 2.2 What Methods are Used to Identify Pathogenic Fungi?; 2.2.1 Culture and Identification; 2.3 Laboratory Detection, Recovery, and Identification of Fungi in the Clinical Microbiology Laboratory; 2.3.1 The Laboratory Manual; 2.3.2 Specimen Collection; 2.3.3 Direct Examination; 2.3.4 Histopathology; 2.3.5 Culture 2.3.6 Storage and Cryopreservation of Cultures for QA and QC in the Clinical Mycology Laboratory |
| Record Nr. | UNINA-9910814256903321 |
|
Reiss Errol
|
||
| Hoboken, N.J., : Wiley-Blackwell, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Larone's medically important fungi : a guide to identification / / Thomas J. Walsh, MD, PhD (hon), FIDSA, FAAM, FECMM, Randall T. Hayden, M.D., Davise H. Larone, MT(ASCP), Ph.D., F(AAM) ; illustrated by Davise H. Larone
| Larone's medically important fungi : a guide to identification / / Thomas J. Walsh, MD, PhD (hon), FIDSA, FAAM, FECMM, Randall T. Hayden, M.D., Davise H. Larone, MT(ASCP), Ph.D., F(AAM) ; illustrated by Davise H. Larone |
| Autore | Walsh Thommie |
| Edizione | [Sixth edition.] |
| Pubbl/distr/stampa | Washington, DC : , : ASM Press, , [2018] |
| Descrizione fisica | 1 online resource (553 pages) |
| Disciplina | 616.9/6901 |
| Soggetto topico |
Pathogenic fungi
Fungi - Cultures and culture media Medical mycology |
| ISBN |
1-68367-296-8
1-68367-053-1 1-55581-988-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910555290803321 |
|
Walsh Thommie
|
||
| Washington, DC : , : ASM Press, , [2018] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Larone's medically important fungi : a guide to identification / / Thomas J. Walsh, MD, PhD (hon), FIDSA, FAAM, FECMM, Randall T. Hayden, M.D., Davise H. Larone, MT(ASCP), Ph.D., F(AAM) ; illustrated by Davise H. Larone
| Larone's medically important fungi : a guide to identification / / Thomas J. Walsh, MD, PhD (hon), FIDSA, FAAM, FECMM, Randall T. Hayden, M.D., Davise H. Larone, MT(ASCP), Ph.D., F(AAM) ; illustrated by Davise H. Larone |
| Autore | Walsh Thommie |
| Edizione | [Sixth edition.] |
| Pubbl/distr/stampa | Washington, DC : , : ASM Press, , [2018] |
| Descrizione fisica | 1 online resource (553 pages) |
| Disciplina | 616.9/6901 |
| Soggetto topico |
Pathogenic fungi
Fungi - Cultures and culture media Medical mycology |
| ISBN |
1-68367-296-8
1-68367-053-1 1-55581-988-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910809867803321 |
|
Walsh Thommie
|
||
| Washington, DC : , : ASM Press, , [2018] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Larone's medically important fungi : a guide to identification / / Lars F. Westblade [and three others]
| Larone's medically important fungi : a guide to identification / / Lars F. Westblade [and three others] |
| Autore | Westblade Lars F (Lars Frederick), <1976-> |
| Edizione | [Seventh edition.] |
| Pubbl/distr/stampa | Hoboken, NJ : , : Wiley-ASM Press, , [2023] |
| Descrizione fisica | 1 online resource (555 pages) |
| Disciplina | 616.9/6901 |
| Soggetto topico |
Fungi - Cultures and culture media
Fungi - Cytochemistry |
| ISBN |
1-68367-443-X
1-68367-441-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Cover -- Title Page -- Copyright -- Contents -- List of Tables -- Preface to the Seventh Edition -- Preface to the First Edition -- Acknowledgments -- About the Authors -- Basics -- How To Use the Guide -- Use of Reference Laboratories and Regulations for Transport -- Safety Precautions -- Taxonomy and Nomenclature -- PART I Direct Microscopic Examination of Clinical Specimens -- Introduction -- Histological Terminology -- Tissue Reactions to Fungal Infection -- Stains -- TABLE 1.1 Histochemical stains for fungi and/or filamentous bacteria in tissue -- Guide to Interpretation of Direct Microscopic Examination -- Detailed Descriptions -- Actinomycosis -- Mycetoma (Actinomycotic or Eumycotic) -- Nocardiosis -- Mucormycosis (Zygomycosis) -- Aspergillosis -- Miscellaneous Hyalohyphomycoses (Other than Aspergillosis) -- Dermatophytosis (Tinea, Ringworm) -- Tinea versicolor -- Tinea nigra -- Phaeohyphomycosis -- Chromoblastomycosis -- Sporotrichosis -- Histoplasmosis -- Emergomycosis -- Talaromycosis (Penicilliosis) -- Blastomycosis -- Paracoccidioidomycosis -- Lobomycosis -- Candidiasis -- Trichosporonosis -- Cryptococcosis -- Pneumocystosis -- Protothecosis -- Coccidioidomycosis -- Rhinosporidiosis -- Adiaspiromycosis -- PART II Identification of Fungi in Culture -- Guide to Identification of Fungi in Culture -- Detailed Descriptions -- Filamentous Bacteria -- Introduction to Filamentous Bacteria -- TABLE 2.1 Differentiation of filamentous aerobic actinomycetes encountered in clinical specimens -- Nocardia spp. -- Streptomyces spp. -- Actinomadura spp. -- Nocardiopsis dassonvillei -- Yeasts and Yeastlike Organisms -- Introduction to Yeasts and Yeastlike Organisms -- Candida albicans -- TABLE 2.2 Characteristics of the genera of clinically encountered yeasts and yeastlike organisms -- Candida dubliniensis.
TABLE 2.3 Characteristics of Candida spp. most commonly encountered in the clinical laboratory -- TABLE 2.4 Characteristics that assist in differentiating Candida dubliniensis from -- Candida tropicalis -- Candida parapsilosis species complex -- Candida lusitaniae -- Candida krusei -- TABLE 2.5 Differentiating characteristics of Magnusiomyces capitatus (formerly Blastoschizomyces capitatus) versus Candida krusei -- TABLE 2.6 Differentiating characteristics of Candida krusei, Candida inconspicua, and Candida norvegensis -- Candida kefyr -- Candida rugosa species complex -- Candida guilliermondii species complex -- TABLE 2.7 Differentiating characteristics of Candida guilliermondii versus Candida famata -- Candida lipolytica -- Candida zeylanoides -- Candida glabrata species complex -- Candida auris -- Candida haemulonii species complex -- Candida pelliculosa -- Cryptococcus neoformans species complex -- Cryptococcus gattii species complex -- TABLE 2.8 Characteristics of Cryptococcus spp. and former members of the genus -- TABLE 2.9 Characteristics of yeasts and yeastlike organisms other than Candida spp. and Cryptococcus spp. -- Rhodotorula and Cystobasidium spp. -- Sporobolomyces salmonicolor -- Saccharomyces cerevisiae -- Malassezia spp. -- Malassezia pachydermatis -- Ustilago spp. -- Prototheca spp. -- Trichosporon and Cutaneotrichosporon spp. -- TABLE 2.10 Key characteristics of the most common clinically encountered Trichosporon spp. and Cutaneotrichosporon spp. -- Magnusiomyces capitatus -- Geotrichum candidum -- Thermally Dimorphic and/or Endemic Fungi -- Introduction to Thermally Dimorphic and/or Endemic Fungi -- Histoplasma capsulatum -- Emergomyces spp. -- Blastomyces dermatitidis/gilchristii -- Coccidioides immitis/posadasii -- Paracoccidioides brasiliensis -- Talaromyces marneffei -- Sporothrix schenckii species complex. Emmonsia crescens -- Thermally Monomorphic Moulds -- Mucormycetes -- Introduction to Mucormycetes -- TABLE 2.11 Differential characteristics of similar organisms in the class Mucormycetes -- TABLE 2.12 Differential characteristics of the clinically encountered Rhizopus spp. -- Rhizopus spp. -- Mucor spp. -- Rhizomucor spp. -- Lichtheimia corymbifera species complex -- Apophysomyces elegans species complex -- Saksenaea vasiformis -- Cokeromyces recurvatus -- Cunninghamella bertholletiae -- Syncephalastrum racemosum -- Basidiobolus spp. -- Conidiobolus coronatus -- Dematiaceous Fungi -- Introduction to Dematiaceous Fungi -- Fonsecaea spp. -- Myrmecridium schulzeri -- Rhinocladiella mackenziei -- Phialophora verrucosa -- TABLE 2.13 Characteristics of Phialophora, Pleurostoma (formerly Pleurostomophora), Phaeoacremonium, Acremonium and Sarocladium, Phialemonium, and Coniochaeta (formerly Lecythophora) -- Pleurostoma richardsiae -- Phaeoacremonium parasiticum -- Phialemonium spp. -- Cladosporium spp. -- TABLE 2.14 Characteristics of Cladosporium spp. and Cladophialophora spp. -- Cladophialophora carrionii -- Cladophialophora boppii -- Cladophialophora bantiana -- Scedosporium apiospermum species complex -- TABLE 2.15 Differentiating phenotypic characteristics of the clinically encountered members of Scedosporium spp. and Lomentospora prolificans -- Lomentospora prolificans -- Verruconis gallopava -- TABLE 2.16 Characteristics of some of the "black yeasts" 246 -- Exophiala jeanselmei species complex -- Exophiala dermatitidis -- Hortaea werneckii -- Madurella mycetomatis -- Trematosphaeria grisea -- Piedraia hortae -- Aureobasidium pullulans -- TABLE 2.17 Differential characteristics of Aureobasidium pullulans versus Hormonema dematioides -- Hormonema dematioides -- Neoscytalidium dimidiatum -- Botrytis cinerea -- Stachybotrys chartarum. Thermothelomyces thermophilus -- Curvularia spp. -- TABLE 2.18 Characteristics of Curvularia spp. and Exserohilum rostratum -- Exserohilum rostratum -- Helminthosporium spp. -- Alternaria spp. -- Stemphylium spp. -- Pseudopithomyces spp. -- Epicoccum spp. -- Nigrospora spp. -- Chaetomium spp. -- Phoma spp. -- Dermatophytes -- Introduction to Dermatophytes -- Latin Terms for Dermatophyte Infections -- Microsporum audouinii -- Microsporum canis -- Paraphyton cookei species complex -- Nannizzia gypsea species complex -- Lophophyton gallinae -- Nannizzia nana -- Microsporum ferrugineum -- Trichophyton mentagrophytes species complex -- TABLE 2.19 Differentiation of similar conidia-producing Trichophyton spp. and Arthroderma spp. -- Trichophyton rubrum -- Trichophyton tonsurans -- Arthroderma terrestre species complex -- Trichophyton megninii -- Trichophyton soudanense -- TABLE 2.20 Growth patterns of Trichophyton spp. and Arthroderma spp. on nutritional test media -- Trichophyton schoenleinii -- Trichophyton verrucosum -- Trichophyton violaceum -- Arthroderma uncinatum -- Epidermophyton floccosum -- Hyaline Hyphomycetes -- Introduction to Hyaline Hyphomycetes -- Fungi in Which Arthroconidia Predominate -- TABLE 2.21 Differential characteristics of fungi in which arthroconidia predominate -- Malbranchea spp. -- Pseudogymnoascus pannorum -- Arthrographis kalrae -- Hormographiella aspergillata -- Common Species of Aspergillus -- The genus Aspergillus -- TABLE 2.22 Differentiating characteristics of the most common Aspergillus spp. -- Aspergillus fumigatus species complex -- Aspergillus niger species complex -- Aspergillus flavus species complex -- Aspergillus versicolor species complex -- Aspergillus ustus species complex -- Aspergillus tanneri -- Aspergillus nidulans species complex -- Aspergillus glaucus -- Aspergillus terreus species complex. Aspergillus clavatus -- Penicillium spp. -- Paecilomyces variotii -- Rasamsonia argillacea species complex -- Purpureocillium lilacinum -- TABLE 2.23 Differential characteristics of Paecilomyces variotii, Rasamsonia argillacea, and Purpureocillium lilacinum -- Scopulariopsis spp. -- TABLE 2.24 Differential characteristics of Scopulariopsis brevicaulis versus Scopulariopsis brumptii -- Gliocladium spp. -- Trichoderma spp. -- Metarhizium anisopliae species complex -- Beauveria bassiana -- Verticillium spp. -- Acremonium and Sarocladium spp. -- Fusarium spp. -- Coniochaeta spp. -- Trichothecium roseum -- Chrysosporium spp. -- TABLE 2.25 Differential characteristics of Chrysosporium versus Sporotrichum -- Sporotrichum pruinosum -- Sepedonium spp. -- Chrysonilia sitophila -- Schizophyllum commune -- PART III Basics of Molecular Methods for Fungal dentification -- Introduction -- Fungal Targets -- TABLE 3.1 Frequently used fungal molecular targets and primers for sequence-based species identification -- TABLE 3.2 Examples of fungal molecular targets and primers for multilocus sequence- based species identification -- Classic Molecular Identification Methods -- Polymerase Chain Reaction -- Non-Sequencing-Based Identification Methods -- MALDI-TOF Mass Spectrometry -- Signal Amplification Methods -- PNA FISH -- Nucleic Acid Amplification Methods -- T2 Magnetic Resonance -- Broad-Panel Molecular Testing and Other Emerging Sample-to-Answer Technologies -- Sequencing-Based Identification Methods -- Sanger Sequencing -- TABLE.3.3 Lane construction for traditional bidirectional Sanger sequencing -- Massive Parallel or Next-Generation Sequencing -- Applications of DNA Sequencing -- Accurate Molecular Identification -- TABLE.3.4 Commonly used databases for identification of medically important fungi -- Phylogenetic Analysis -- Organism Typing. Detection of Genetic Determinants of Resistance. |
| Record Nr. | UNINA-9910830946903321 |
|
Westblade Lars F (Lars Frederick), <1976->
|
||
| Hoboken, NJ : , : Wiley-ASM Press, , [2023] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Medical mycology : cellular and molecular techniques / / edited by Kevin Kavanagh
| Medical mycology : cellular and molecular techniques / / edited by Kevin Kavanagh |
| Pubbl/distr/stampa | Chichester, England ; ; Hoboken, NJ, : Wiley, c2007 |
| Descrizione fisica | 1 online resource (350 p.) |
| Disciplina | 616.9/6901 |
| Altri autori (Persone) | KavanaghKevin |
| Soggetto topico |
Medical mycology
Mycoses - Molecular aspects |
| ISBN |
1-282-34592-3
9786612345920 0-470-05741-6 0-470-05740-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Medical Mycology; Contents; Preface; List of Contributors; 1 Diagnosis of Candida Infection in Tissue by Immunohistochemistry; 1.1 Introduction; 1.2 Specificity of monoclonal antibody 3H8 for C. albicans; Protocol 1.1 Testing of specificity of monoclonal antibody 3H8; 1.3 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms; Protocol 1.2 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms; 1.4 Application of immunohistochemistry in the diagnosis of Candida periodontal disease
Protocol 1.3 Use of monoclonal antibody 3H8 in the detection of C. albicans in tissue1.5 References; 2 Transmission Electron Microscopy of Pathogenic Fungi; 2.1 Introduction; 2.2 Glutaraldehyde-potassium-permanganate or glutaraldehyde-osmiumtetroxide fixation for ultrastructural analysis; Protocol 2.1 Glutaraldehyde-osmium tetroxide (#) or glutaraldehyde-potassium permanganate (*) fixation for ultrastructural analysis; 2.3 Identification of the different compartments of the secretory pathway in yeasts Protocol 2.2 Identification of the different compartments of the secretory pathway in yeasts2.4 Cytochemical localization of acid phosphatase in yeasts; Protocol 2.3 Cytochemical localization of acid phosphatase in yeasts; 2.5 Detection of anionic sites; Protocol 2.4 Detection of anionic sites; 2.6 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg); Protocol 2.5 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg) 2.7 Enzyme-gold approach for the detection of polysaccharides in the cell wallProtocol 2.6 Enzyme-gold approach for the detection of polysaccharides in the cell wall; 2.8 Detection of glycoconjugates by the lectin-gold technique; Protocol 2.7 Detection of glycoconjugates by the lectin-gold technique; 2.9 Immunogold detection of antigens on ultrathin sections of acrylic resin; Protocol 2.8 Immunogold detection of antigens on ultrathin sections of acrylic resin; 2.10 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection Protocol 2.9 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection2.11 Overview; 2.12 References; 3 Evaluation of Molecular Responses and Antifungal Activity of Phagocytes to Opportunistic Fungi; 3.1 Introduction; 3.2 Preparation of conidia and hyphae of opportunistic fungi; Protocol 3.1 Preparation of conidia and hyphae of opportunistic fungi; Protocol 3.2 Preparation of hyphal fragments; 3.3 Isolation of human monocytes from whole blood; Protocol 3.3 Isolation of human MNCs from whole blood; 3.4 Analysis of human MNC function in response to fungal infection Protocol 3.4 XTT microassay |
| Record Nr. | UNINA-9910876940203321 |
| Chichester, England ; ; Hoboken, NJ, : Wiley, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||