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Assay development [[electronic resource] ] : fundamentals and practices / / Ge Wu
| Assay development [[electronic resource] ] : fundamentals and practices / / Ge Wu |
| Autore | Wu Ge <1962-> |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, c2010 |
| Descrizione fisica | 1 online resource (425 p.) |
| Disciplina | 612/.015 |
| Soggetto topico |
Biological assay
Aquatic biology |
| ISBN |
1-282-65651-1
9786612656514 0-470-58312-6 0-470-58311-8 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910140739303321 |
Wu Ge <1962->
|
||
| Hoboken, N.J., : Wiley, c2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Assay development : fundamentals and practices / / Ge Wu
| Assay development : fundamentals and practices / / Ge Wu |
| Autore | Wu Ge <1962-> |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, c2010 |
| Descrizione fisica | 1 online resource (425 p.) |
| Disciplina | 612/.015 |
| Soggetto topico |
Biological assay
Aquatic biology |
| ISBN |
9786612656514
9781282656512 1282656511 9780470583128 0470583126 9780470583111 0470583118 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Intro -- ASSAY DEVELOPMENT -- CONTENTS -- FOREWORD -- PREFACE -- ACKNOWLEDGMENTS -- ABBREVIATIONS -- 1 INTRODUCTION TO ASSAY DEVELOPMENT -- 1.1 Assay and Bioassay -- 1.2 Drug Discovery Process and Role of Assays in the Process -- 1.3 Bioassay Development -- 1.4 Bioassay Classifications -- Useful Websites -- Bibliography -- 2 MEASUREMENT AND INSTRUMENTATION -- 2.1 Measurement and Perturbation -- 2.2 Common Instrumental Methods and Instrument Components -- 2.3 Molecular Absorption Measurements -- 2.4 Molecular Luminescence Measurements -- 2.5 Luminescence Lifetime Measurement and Time-Resolved Fluorescence Measurement -- 2.6 Fluorescence Resonance Energy Transfer (FRET) and Time-Resolved-FRET -- 2.7 Fluorescence Quenching -- 2.8 Fluorescence Polarization (FP) -- 2.9 Radioactivity Measurement -- 2.10 Evaluating and Selecting an Instrumental Method for Bioassay -- Useful Websites -- Bibliography -- 3 FUNDAMENTAL PRINCIPLES OF ASSAY DEVELOPMENT WITH ISOLATED PROTEINS -- 3.1 Chemical Potential, Equilibrium, and Kinetics -- 3.2 Protein Binding Studies at Equilibrium -- 3.3 Kinetic Studies of the Protein Binding Process -- 3.4 Enzyme Kinetics -- 3.5 Inhibition of Protein Function -- 3.6 Assay Development with Isolated Proteins -- Useful Websites -- Bibliography -- 4 SEPARATION-BASED TECHNIQUES IN BIOASSAYS -- 4.1 Washing Solid Supports to Remove Impurities -- 4.2 Organic Solvent Extraction of Hydrophobic Molecules -- 4.3 Centrifugation to Remove Dense Particles -- 4.4 Membrane Filtration -- 4.5 Liquid Chromatography -- 4.6 Electrophoresis -- Useful Websites -- Bibliography -- 5 GENERAL PROTEIN BINDING ASSAY FORMATS -- 5.1 Equilibrium Dialysis -- 5.2 Competitive Binding Assays with Radioactive or Other Labeled Ligands -- 5.3 Application of SPA and FlashPlate in Binding Studies -- 5.4 Application of Fluorescence Polarization (FP) in Binding Studies.
5.5 Application of FRET Assays in Binding Studies -- 5.6 Application of ELISA in Binding Studies -- 5.7 Surface Plasmon Resonance (SPR) Technology and Its Application in Binding Studies -- 5.8 Application of Label-Free Technologies in Binding Studies -- Useful Websites -- Bibliography -- 6 FUNCTIONAL ASSAYS WITH ISOLATED PROTEASES -- 6.1 Introduction to Proteases and Their Substrates -- 6.2 Function of Proteases and Their Role in Drug Discovery -- 6.3 Protease Assays -- 6.4 Protease Substrate Profiling -- 6.5 Protease Inhibitors -- 6.6 Assay Development for Caspases with a Fluorogenic Substrate -- 6.7 Assay Development for Carboxypeptidase U (EC 3.4.17.20) -- Useful Websites for Proteases -- Bibliography -- 7 FUNCTIONAL ASSAYS FOR PROTEIN KINASES -- 7.1 Introduction to Protein Kinases -- 7.2 Substrates for In Vitro Kinase Assays -- 7.3 Kinase Assay Development Strategies -- 7.4 Kinase Assay Based on Detection of Phosphorylated Product -- 7.5 Kinase Assays by Measuring the Generation of ADP -- 7.6 Kinase Assays by Measuring the Depletion of ATP -- 7.7 Kinase Assays by Measuring the Depletion of Peptide Substrate -- 7.8 Kinase Assays by Simultaneous Measurement of Both Product and Substrate -- 7.9 Example of a Kinase Assay Development in HTRF Format -- Useful Websites -- Bibliography -- 8 FUNDAMENTAL PRINCIPLES OF CELL-BASED ASSAYS -- 8.1 Cell Signaling, Signal Transduction, and Cellular Responses -- 8.2 General Approaches in Cell-Based Assays -- 8.3 Concept of Affinity and Efficacy in Cell-Based Assays -- 8.4 Development of Cell-Based Assays -- Useful Websites -- Bibliography -- 9 FUNCTIONAL ION CHANNEL ASSAYS -- 9.1 Introduction to Ion Channels -- 9.2 Strategies for Ion Channel Assays -- 9.3 Electrophysiological Methods -- 9.4 Ion Flux Methods -- 9.5 Membrane Potential Sensing Methods -- 9.6 Selecting Suitable Assays for Ion Channel Studies. Useful Websites and Vendors -- Bibliography -- 10 ASSAYS WITH GPCRs -- 10.1 Introduction to GPCRs and G Proteins -- 10.2 G Protein-Coupled Receptor Activation and Signal Transduction -- 10.3 Strategies of GPCR Assay Development -- 10.4 G Protein-Coupled Receptor Assays by Measuring the Extent of GTP Binding to G(α) -- 10.5 G Protein-Coupled Receptor Assays Based on Measurement of cAMP -- 10.6 G Protein-Coupled Receptor Assays Based on Measurement of Intracellular Inositol Phospholipids -- 10.7 G Protein-Coupled Receptor Assays Based on Measurement of Intracellular Ca(2+) -- 10.8 G Protein-Coupled Receptor Assays Based on Measurement of MAPK Activity -- 10.9 G Protein-Coupled Receptor Assays with Reporter Gene -- 10.10 G Protein-Coupled Receptor Assays by Monitoring Events Leading to GPCR Internalization -- Useful Websites -- Bibliography -- 11 ASSAYS BASED ON INTEGRATED CELL SYSTEM PROPERTIES -- 11.1 Cell Viability, Proliferation, and Cytotoxicity Assays -- 11.2 Measurement of Extracellular Indicators of Cellular Metabolism -- 11.3 Measurement of Cell's Effect on Electrical Impedance -- 11.4 Measurement of Protein Secretion from Cells -- 11.5 Measurement of Discoloration of Melanophore Cells -- 11.6 Measurement of Cell Motility -- Useful Websites -- Bibliography -- 12 HIGH-CONTENT CELL-BASED ASSAY WITH OPTICAL IMAGING TECHNIQUES -- 12.1 Sample Preparation -- 12.2 Cellular Image Collection -- 12.3 Image Abstraction, Analysis, and Data Management -- 12.4 Applications of iCHCS -- Useful Websites -- Bibliography -- 13 HIGH-THROUGHPUT SCREENING -- 13.1 Introduction -- 13.2 Molecular or Cellular Targets and Assay Development -- 13.3 Compound Library Management -- 13.4 Hardware Module -- 13.5 Software Module -- 13.6 HTS Operation Management -- 13.7 Building an HTS Operation for Biopharmaceutical Discovery. 13.8 Quality Control and Data Analysis in Primary Screening -- Useful Websites -- Bibliography -- 14 CASE STUDY: DEVELOPMENT OF A MICROFLUIDIC-BASED KINASE ASSAY PLATFORM -- 14.1 Background of Microfluidic Technology and Its Application in Bioassays -- 14.2 The Original Mobility Shift Kinase Assay Format -- 14.3 Realizing the Flaws in the Original Kinase Assay Format -- 14.4 Searching for Alternative Kinase Assay Methods -- 14.5 Development of the Off-Chip Kinase Assay Format -- 14.6 Current Stage of Microfluidic Technology in Bioassays -- 14.7 Appendix: Poster Presented at the 2002 Society for Biomolecular Screening Annual Meeting: Analysis of Mobility Shift Data Obtained from Labchip in Kinase Assay -- Useful Websites -- Bibliography -- INDEX. |
| Record Nr. | UNINA-9910817368703321 |
|
Wu Ge <1962->
|
||
| Hoboken, N.J., : Wiley, c2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biological aspects of human health and well-being [[electronic resource] /] / Tsisana Shartava, editor
| Biological aspects of human health and well-being [[electronic resource] /] / Tsisana Shartava, editor |
| Pubbl/distr/stampa | New York, : Nova Science Publishers, c2011 |
| Descrizione fisica | 1 online resource (291 p.) |
| Disciplina | 612/.015 |
| Altri autori (Persone) | ShartavaTsisana |
| Collana | Medicine and biology research developments |
| Soggetto topico |
Biochemistry
Clinical biochemistry |
| Soggetto genere / forma | Electronic books. |
| ISBN | 1-61470-792-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910457673503321 |
| New York, : Nova Science Publishers, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biological aspects of human health and well-being [[electronic resource] /] / Tsisana Shartava, editor
| Biological aspects of human health and well-being [[electronic resource] /] / Tsisana Shartava, editor |
| Pubbl/distr/stampa | New York, : Nova Science Publishers, c2011 |
| Descrizione fisica | 1 online resource (291 p.) |
| Disciplina | 612/.015 |
| Altri autori (Persone) | ShartavaTsisana |
| Collana | Medicine and biology research developments |
| Soggetto topico |
Biochemistry
Clinical biochemistry |
| ISBN | 1-61470-792-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910781953703321 |
| New York, : Nova Science Publishers, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biological aspects of human health and well-being / / Tsisana Shartava, editor
| Biological aspects of human health and well-being / / Tsisana Shartava, editor |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | New York, : Nova Science Publishers, c2011 |
| Descrizione fisica | 1 online resource (291 p.) |
| Disciplina | 612/.015 |
| Altri autori (Persone) | ShartavaTsisana |
| Collana | Medicine and biology research developments |
| Soggetto topico |
Biochemistry
Clinical biochemistry |
| ISBN | 1-61470-792-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Intro -- BIOLOGICAL ASPECTS OF HUMAN HEALTH AND WELL-BEING -- BIOLOGICAL ASPECTS OF HUMAN HEALTH AND WELL-BEING -- CONTENTS -- PREFACE -- Chapter I CONTROL OF EMERGING INFECTIOUS AGENTS CAUSING NOSOCOMIAL AND COMMUNITY-ACQUIRED CROSS INFECTIONS IN IMMUNOCOMPROMISED HOSTS. -- COMMENTARY -- REFERENCES -- Chapter II GENETIC TOOLS APPLICATIONS TO BIOTECHNOLOGY OF CYANOBACTERIA -- ABSTRACT -- INTRODUCTION -- CYANOBACTERIA ARE SOURCE OF A LARGE VARIETY OF BIOCOMPOUNDS -- CYANOBACTERIA ARE PROMISING PRODUCERS OF MOLECULAR HYDROGEN - FUTURE ECOLOGICALY PURE FUEL -- CYANOBACTERIA AND ENVIRONMENT -- CYANOBACTERIA AND NANOTECHNOLOGY -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter III WHY GLUCOSE IS THE PRINCIPAL SOURCE OF ENERGY FOR LIVING BEINGS? AND THE EXPLANATION OF HUMAN DISEASES -- DEDICATION -- INTRODUCTION -- THE HYPOTHESIS OF THE BIOLOGICAL COMPETITION AND ITS CONSEQUENCES -- EXPLANATION OF HUMAN DISEASES BASED ON THE BIOLOGICAL COMPETITION -- DISCUSSION -- CONCLUSION -- REFERENCES -- Chapter IV THE EVOLUTION BIOLOGY OF HEALTH AND DISEASE CLINICAL MEDICINE AS SEEN FROM A DARWINIAN PERSPECTIVE -- ABSTRACT -- INTRODUCTION -- AUGMENTING MEDICINE WITH EVOLUTIONARY INSIGHTS -- WHY NATURAL SELECTION HAS LEFT US VULNERABLE TO DISEASE -- ALLERGY AND CANCER: A COMPLEX RELATIONSHIP -- GENE/ENVIRONMENT INTERACTION IN ATHEROSCLEROSIS -- ECOLOGY OF ANTIBIOTIC RESISTANCE -- EXPERIMENTAL EVOLUTION -- THE POLYMORPHIC MAJOR HISTOCOMPATIBILITY COMPLEX -- "RECENT" EVOLUTION IN HOMO SAPIENS -- CONCLUSION -- REFERENCES -- Chapter V A QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP FOR THE GASTROPROTECTIVE EFFECT OF FLAVONOIDS EVALUATED IN HUMAN COLON ADENOCARCINOMA HT-29 CELLS -- ABSTRACT -- INTRODUCTION -- CHEMICAL STRUCTURE OF FLAVONOIDS -- PHYSICOCHEMICAL PROPERTIES OF FLAVONOIDS -- Bond Dissociation Energy (BDE) -- Lipophilicity (log P).
CYTOPROTECTION ASSAY -- MATERIALS AND METHODS -- Materials -- Assessment of Cell Viability -- Culture and Treatment of HT-29 Cells -- Annexin V Staining and Flow Cytometric Analysis -- CALCULATION OF RESULTS -- Cell Death Index (CDI) -- Calculation of 50% Reduction in Cell Death (EC50) -- QUANTUM CHEMICAL CALCULATIONS -- Calculation of Heat of Formation -- Calculation of Log P -- RESULTS AND DISCUSSION -- Cytotoxic effects of H2O2 -- Cytotoxicity of Flavonoids -- The Influence of Trolox on Cytotoxic Effects of H2O2 -- STRUCTURAL RELATED CYTOPROTECTIVE ACTIVITY OF FLAVONOIDS -- Effects of Hydroxyl Groups in the B Ring -- Effect of the 3-OH Group, 2,3-Double Bond and 4-Keto Group -- Effect of the Carbohydrate Moieties -- CYTOPROTECTIVE AND PHYSICOCHEMICAL PROPERTIES OF FLAVONOIDS -- Correlation between O-H Bond Dissociation Enthalpy (BDE) and Cytoprotective Activity (EC50) of Flavonoids -- Correlation between Partition Coefficient (Log P) and Cytoprotective Activity (EC50) of Flavonoids -- Quantitative Structure-Activity Relationship (QSAR) Model -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter VI SIALYLATION MECHANISM IN BACTERIA: FOCUSED ON CMP-N-ACETYLNEURAMINIC ACID SYNTHETASES AND SIALYLTRANSFERASES -- ABSTRACT -- INTRODUCTION -- BIOSYNTHESIS OF SIALIC ACIDS AND SIALYL-GLYCOCONJUGATES -- CMP-Neu5Ac Synthetase -- Origin of Bacterial CMP-Neu5Ac Synthetase -- Features of Bacterial CMP-Neu5Ac Synthetases -- SIALYLTRANSFERASES -- Origin and Classification of the Bacterial Sialyltransferases -- Tertiary Structure of Bacterial Sialyltransferases -- Reaction of GT Family 80 Sialyltransferases -- Sialyltransferases Belong to GT Family 80 -- α2,6-SialyltransferaseObtained from P. damselae -- α2,6-SialyltransferaseObtained from P. leiognathi -- α/β-Galactoside α2,3-sialyltransferase from Vibrio sp. Multi-Functional α2,3 Sialyltransferase from P. multocida -- CONCLUSION -- REFERENCES -- Chapter VII THE HEPATOCELLULAR DYSFUNCTION CRITERIA: HEPATOCYTE CARBOHYDRATE METABOLIZING ENZYMES AND KUPFFER CELL LYSOSOMAL ENZYMES IN 2'NITROIMIDAZOLE EFFECT ON AMOEBIC LIVER ABSCESS (ELECTRON MICROSCOPIC - ENZYME APPROACH) -- ABSTRACT -- INTRODUCTION -- METHODS AND MATERIALS -- RESULTS -- DISCUSSION -- CHALLENGES, LIMITATIONS AND FUTURISTIC APPROACHES -- CONCLUSION -- ACKNOWLEDGMENT -- REFERENCES -- Chapter VIII THE EFFECT OF NITROIMIDAZOLE ON GLUCOKINASE ENZYME REGULATORY PROPERTIES: GLUCOKINASE AS BIOSENSOR# -- ABSTRACT -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS -- DISCUSSION -- REFERENCES -- Chapter IX POST-TRANSCRIPTIONAL EFFECTS OF ESTROGENS ON GENE EXPRESSION: MESSENGER RNA STABILITY AND TRANSLATION REGULATED BY MICRORNAS AND OTHER FACTORS -- ABSTRACT -- INTRODUCTION -- ESTROGENS STABILIZE AND DESTABILIZE SPECIFIC MRNAS -- ESTROGENS AFFECT MRNA TRANSLATION -- ESTROGEN ACTIONS AND MICRORNAS -- MicroRNAs - Biogenesis and Regulation of Gene Expression -- Estrogens Regulate Expression of MicroRNA Genes in Responsive Tissues During Normal Physiology and Disease -- MicroRNAs Regulate ER Gene Expression and Estrogen Actions -- FUTURE THERAPEUTIC APPROACHES TO REGULATING POST-TRANSCRIPTIONAL ESTROGEN ACTIONS -- CONCLUSION -- REFERENCES -- Chapter X EPIGENETICS OF GESTATIONAL TROPHOBLASTIC DISEASE: GENOMIC IMPRINTING AND X CHROMOSOME INACTIVATION -- ABSTRACT -- INTRODUCTION -- PLACENTA DEVELOPMENT AND TROPHOBLASTIC CELLS -- GENOMIC IMPRINTING AND PLACENTAL DEVELOPMENT -- GESTATIONAL TROPHOBLASTIC DISEASES: ABNORMAL GENOMES AND GENOMIC IMPRINTING -- X CHROMOSOME INACTIVATION AND PLACENTAL DEVELOPMENT -- PATERNAL X CHROMOSOME IN THE PATHOGENESIS OF HUMAN TROPHOBLASTIC DISEASES -- CONCLUSION -- REFERENCES. Chapter XI THE ROLE OF SUPRASPINAL GABA AND GLUTAMATE IN THE MEDIATION AND MODULATION OF PAIN -- ABSTRACT -- ABBREVIATIONS -- INTRODUCTION -- THE GABAERGIC AND GLUTAMATERGIC SYSTEMS -- GABA RECEPTORS -- GABAA Receptor Subunit Composition -- GABAB Receptor Subunit Composition -- IONOTROPIC GLUTAMATE RECEPTORS -- NMDA Receptor Subunit Composition -- AMPA Receptor Subunit Composition -- Kainate Receptor Subunit Composition -- METABOTROPIC GLUTAMATE RECEPTORS -- SOME IMPORTANT CONSIDERATIONS -- THE ROLE OF SUPRASPINAL GABAERGIC AND GLUTAMATERGIC SYSTEMS DURING PAIN -- PLASTICITY OF SUPRASPINAL GABAERGIC AND GLUTAMATERGIC SYSTEMS DURING PAIN -- Changes in Activity of Supraspinal GABA and Glutamate Neurons in Animal Models of Acute Pain -- Changes in Activity of Supraspinal GABA and Glutamate Neurons in Animal Models of Inflammatory and Neuropathic Pain -- Brain Regional Alterations in Levels of GABA and Glutamate or Their Receptors in Animal Models of Inflammatory and Neuropathic Pain -- PHARMACOLOGICAL ELUCIDATION OF THE ROLES OF SUPRASPINAL IONOTROPIC AND METABOTROPIPC GABA AND GLUTAMATE RECEPTOR SUBTYPES IN ANIMAL MODELS OF ACUTE PAIN -- Effects of Supraspinal Administration of Agents Acting at GABAA Receptors in Animal Models of Acute Pain -- Effects of Supraspinal Administration of Agents Acting at GABAB Receptors in Animal Models of Acute Pain -- Effects of Supraspinal Administration of Agents Acting at Ionotropic Glutamate Receptors in Animal Models of Acute Pain -- Effects of Supraspinal Administration of Agents Acting at Metabotropic Glutamate Receptors in Animal Models of Acute Pain -- PHARMACOLOGICAL ELUCIDATION OF THE ROLES OF SUPRASPINAL IONOTROPIC AND METABOTROPIPC GABA AND GLUTAMATE RECEPTOR SUBTYPES IN ANIMAL MODELS OF INFLAMMATORY AND NEUROPATHIC PAIN. Effects of Supraspinal Administration of Agents Acting at GABAA Receptors in Animal Models of Inflammatory and Neuropathic Pain -- Effects of Supraspinal Administration of Agents Acting at GABAB Receptors in Animal Models of Inflammatory and Neuropathic Pain -- Effects of Supraspinal Administration of Agents Acting at Ionotropic Glutamate Receptors in Animal Models of Inflammatory and Neuropathic Pain -- Effects of Supraspinal Administration of Agents Acting at Metabotropic Glutamate Receptors in Animal Models of Inflammatory and Neuropathic Pain -- THE ROLE OF CENTRAL GABA AND GLUTAMATE IN THE ANTINOCICEPTIVE EFFECTS OF ANALGESICS -- NSAIDs and Paracetamol -- Opioids -- Cannabinoids -- Anticonvulsants and Antidepressants -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter XII MULTIPHOTON MICROSCOPY OF INTRAVITAL DEEP OCULAR TISSUES -- ABSTRACT -- 1. INTRODUCTION -- 2. MULTIPHOTON IMAGING SYSTEM -- 3. SUBJECTS AND EXAMINATIONS -- 3.1. Corneal and Sclera Examinations -- 3.2. Retinal Examinations -- 4. CORNEAL MULTIPHOTON IMAGING -- 4.1. Optical Overview of Corneal Architecture with Subcellular Resolution Based on Multiphoton Excitation Microscopy/Tomography -- 4.1.1. Multiphoton Autofluorescence Tomography of Corneal Epithelial Layers (At Depths of 10, 25, and 41 Microns, Respectively) -- 4.1.2. Revealing of Stromal Intact Cellular and Collagenous Networks Based on Multiphoton Excitation Imaging -- (i) Selective Displaying of Intratissue Keratocyte Networks in Stroma with Intracellular Spatial Resolution -- (ii) SHG Signal Imaging of Collagen Lamellas in Corneal Stroma -- (iii) Microstructural Co-localization and Topography of Keratocytes and their Hosting Collagen Lamellas in Corneal Stroma Based on 2PF and SHG -- 4.1.3. Multiphoton Excitation Detection of Endothelial Cells at a Corneal Depth of 369 Microns with Living Animals. 4.2. Optical Determination of Bowman's Layer with In-tandem Combination Assistance of 2PF and SHG Signal Imaging. |
| Record Nr. | UNINA-9910960676403321 |
| New York, : Nova Science Publishers, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Chemical biology [[electronic resource] ] : applications and techniques / / [edited by] Banafshé Larijani, Colin A. Rosser, Rudiger Woscholski
| Chemical biology [[electronic resource] ] : applications and techniques / / [edited by] Banafshé Larijani, Colin A. Rosser, Rudiger Woscholski |
| Pubbl/distr/stampa | Chichester, England ; ; Hoboken, NJ, : John Wiley & Sons, c2006 |
| Descrizione fisica | 1 online resource (274 p.) |
| Disciplina | 612/.015 |
| Altri autori (Persone) |
LarijaniBanafshé
RosserColin A WoscholskiRudiger |
| Soggetto topico |
Chemical biology
Microscopy |
| ISBN |
0-470-31925-9
1-280-60617-7 9786610606177 0-470-09066-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Cryomicroscopy -- Atomic force microscopy : applications in biology -- Differential scanning calorimetry in the study of lipid structures -- Membrane potentials and membrane probes -- Identification and quantification of lipids using mass spectrometry -- Liquid-state NMR -- Solid-state NMR in biomembranes -- Molecular dynamics -- Two-dimensional infrared studies of biomolecules -- Biological applications of single- and two-photon fluorescence -- Optical tweezers -- PET imaging in chemical biology -- Chemical genetics. |
| Record Nr. | UNINA-9910143697103321 |
| Chichester, England ; ; Hoboken, NJ, : John Wiley & Sons, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Chemical biology [[electronic resource] ] : applications and techniques / / [edited by] Banafshé Larijani, Colin A. Rosser, Rudiger Woscholski
| Chemical biology [[electronic resource] ] : applications and techniques / / [edited by] Banafshé Larijani, Colin A. Rosser, Rudiger Woscholski |
| Pubbl/distr/stampa | Chichester, England ; ; Hoboken, NJ, : John Wiley & Sons, c2006 |
| Descrizione fisica | 1 online resource (274 p.) |
| Disciplina | 612/.015 |
| Altri autori (Persone) |
LarijaniBanafshé
RosserColin A WoscholskiRudiger |
| Soggetto topico |
Chemical biology
Microscopy |
| ISBN |
0-470-31925-9
1-280-60617-7 9786610606177 0-470-09066-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Cryomicroscopy -- Atomic force microscopy : applications in biology -- Differential scanning calorimetry in the study of lipid structures -- Membrane potentials and membrane probes -- Identification and quantification of lipids using mass spectrometry -- Liquid-state NMR -- Solid-state NMR in biomembranes -- Molecular dynamics -- Two-dimensional infrared studies of biomolecules -- Biological applications of single- and two-photon fluorescence -- Optical tweezers -- PET imaging in chemical biology -- Chemical genetics. |
| Record Nr. | UNINA-9910830213603321 |
| Chichester, England ; ; Hoboken, NJ, : John Wiley & Sons, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Chemical biology : applications and techniques / / [edited by] Banafshe Larijani, Colin A. Rosser, Rudiger Woscholski
| Chemical biology : applications and techniques / / [edited by] Banafshe Larijani, Colin A. Rosser, Rudiger Woscholski |
| Pubbl/distr/stampa | Chichester, England ; ; Hoboken, NJ, : John Wiley & Sons, c2006 |
| Descrizione fisica | 1 online resource (274 p.) |
| Disciplina | 612/.015 |
| Altri autori (Persone) |
LarijaniBanafshé
RosserColin A WoscholskiRudiger |
| Soggetto topico |
Biochemistry
Biology Microscopy |
| ISBN |
9786610606177
9780470319253 0470319259 9781280606175 1280606177 9780470090664 0470090669 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Cryomicroscopy -- Atomic force microscopy : applications in biology -- Differential scanning calorimetry in the study of lipid structures -- Membrane potentials and membrane probes -- Identification and quantification of lipids using mass spectrometry -- Liquid-state NMR -- Solid-state NMR in biomembranes -- Molecular dynamics -- Two-dimensional infrared studies of biomolecules -- Biological applications of single- and two-photon fluorescence -- Optical tweezers -- PET imaging in chemical biology -- Chemical genetics. |
| Record Nr. | UNINA-9911019407903321 |
| Chichester, England ; ; Hoboken, NJ, : John Wiley & Sons, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Ligands, polymers, and amino acids [[electronic resource] /] / Tsisana Shartava, editor
| Ligands, polymers, and amino acids [[electronic resource] /] / Tsisana Shartava, editor |
| Pubbl/distr/stampa | New York, : Nova Science Publishers, Inc., c2011 |
| Descrizione fisica | 1 online resource (176 p.) |
| Disciplina | 612/.015 |
| Altri autori (Persone) | ShartavaTsisana |
| Collana | Biochemistry research trends |
| Soggetto topico |
Biochemistry
Nucleic acids - Metabolism |
| Soggetto genere / forma | Electronic books. |
| ISBN | 1-61209-498-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910461199603321 |
| New York, : Nova Science Publishers, Inc., c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Ligands, polymers, and amino acids [[electronic resource] /] / Tsisana Shartava, editor
| Ligands, polymers, and amino acids [[electronic resource] /] / Tsisana Shartava, editor |
| Pubbl/distr/stampa | New York, : Nova Science Publishers, Inc., c2011 |
| Descrizione fisica | 1 online resource (176 p.) |
| Disciplina | 612/.015 |
| Altri autori (Persone) | ShartavaTsisana |
| Collana | Biochemistry research trends |
| Soggetto topico |
Biochemistry
Nucleic acids - Metabolism |
| ISBN | 1-61209-498-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910789542503321 |
| New York, : Nova Science Publishers, Inc., c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
