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Human molecular biology laboratory manual [[electronic resource] /] / Stefan Surzycki
| Human molecular biology laboratory manual [[electronic resource] /] / Stefan Surzycki |
| Autore | Surzycki Stefan <1936-> |
| Pubbl/distr/stampa | Malden, MA, : Blackwell Pub., 2003 |
| Descrizione fisica | 1 online resource (246 p.) |
| Disciplina |
611.01816
611/.01816 |
| Soggetto topico |
Molecular biology
Human genetics |
| ISBN |
1-281-31269-X
9786611312695 0-470-99925-X 0-470-99924-1 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Human Molecular Biology Laboratory; Contents; Preface; Chapter 1 Preparation of Human Genomic DNA; Introduction; Background; Cell Breakage; Removal of Protein; Deproteinization using organic solvents; Deproteinization using enzymes; Removal of RNA; Concentrating the DNA; Determination of the Purity and Quantity of DNA; First Laboratory Period; Safety precautions; Technical tips; Protocol; Second Laboratory Period; Safety precautions; Protocol; Technical tips; Expected results; References; Chapter 2 DNA Fingerprinting: Multi-locus Analysis; Introduction; Background; First Laboratory Period
Experiment 1: Restriction Enzyme DigestionIntroduction; Background; Technical tips; Protocol; References; Experiment 2: agarose gel electrophoresis; Introduction; Background; Safety precautions; Protocol; References; Second Laboratory Period; Experiment 3: Southern blotting; Introduction; Background; Safety precautions; Technical tips; Protocol; References; Third Laboratory Period; Experiment 4: preparation of probe and hybridization; Introduction; Background; Technical tips; Protocol; References; Fourth Laboratory Period; Protocol; Fifth Laboratory Period; Protocol; Data analysis Chapter 3 DNA Fingerprinting: Single-locus AnalysisIntroduction; Background; First Laboratory Period; Protocol; Second Laboratory Period; Protocol; Data analysis; Expected results; References; General reading; Chapter 4 Out of Africa: Origin of Modern Humans; Introduction; Background; Origin of humans; PCR; First Laboratory Period; Technical tips; Protocol; Second Laboratory Period; Safety precautions; Protocol; Data analysis; Expected results; References; Chapter 5 DNA Sequencing; Introduction; Background; DNA sequencing methods; Sequencing strategies; References; First Laboratory Period Experiment 1: nebulization shearing of DNAIntroduction; Background; Safety precautions; Technical tips; Protocol; Expected results; Experiment 2: repair of the ends of sheared DNA; Introduction; Safety precautions; Technical tips; Protocol; References; Second Laboratory Period; Experiment 3: ligation to sequencing vector; Introduction; Background; Technical tips; Protocol; References; Experiment 4: transformation of bacteria by electroporation; Introduction; Background; Technical tips; Protocol; Expected results; References; Third Laboratory Period Experiment 5: preparation of plasmid for DNA sequencingIntroduction; Background; Technical tips; Protocol; Experiment 6: sequencing reactions for an ABI 3700 sequencer; Introduction; Technical tips; Protocol; Fourth Laboratory Period; Experiment 7: removing dideoxy terminators; Protocol; References; Chapter 6 Computer Analysis of Sequencing Data; Introduction; Background; Databases and sequence formats; Sequence alignments; BLAST; FASTA; BLAST versus FASTA; Single-sequence analysis; Dot matrix analysis; Technical tips; Protocol; References; Sequence Alignment with BLAST Search of ""nr"" database |
| Record Nr. | UNISA-996205987903316 |
Surzycki Stefan <1936->
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| Malden, MA, : Blackwell Pub., 2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
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Industrial scale suspension culture of living cells / / edited by Hans-Peter Meyer and Diego R. Schmidhalter
| Industrial scale suspension culture of living cells / / edited by Hans-Peter Meyer and Diego R. Schmidhalter |
| Pubbl/distr/stampa | Weinheim, Germany : , : Wiley-Blackwell, , 2014 |
| Descrizione fisica | 1 online resource (644 p.) |
| Disciplina |
611
611/.01816 |
| Soggetto topico |
Industrial microbiology - Methodology
Cell culture - Industrial applications Biotechnology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
3-527-68335-6
3-527-68332-1 3-527-68334-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Industrial Scale Suspension Culture of Living Cells; Forword; Contents; Preface; List of Contributors; The History and Economic Relevance of Industrial Scale Suspension Culture of Living Cells; 1 Introduction; 2 A Short History of Suspension Culture (Fermentation); 2.1 Ethanol, Organic Acids, and Solvents, the Beginning; 2.2 Vitamins Fermentation Takes a Long Time to Develop; 2.3 Steroids, the First Large-Scale Biocatalysis Processes; 2.4 Antibiotics, a US-Lead Turning Point in Fermentation Technology; 2.5 Amino Acids, a Japanese Fermentation Success Story
2.6 Enzymes, a European Fermentation Success Story2.7 Single Cell Proteins, an Economic Flop; 2.8 Biofuels are Controversial Story; 2.9 Recombinant DNA Technology Based Products (Monoclonal Antibodies and Other Recombinant Proteins), Setting off an Avalanche of New Products; 3 The Contemporary Situation; 3.1 How Long Can the USA Keep its Leading Role?; 3.2 China and India Become Global Forces in Fermentation; 4 The Future of Suspension Culture; 4.1 New Frontiers; 4.2 Yet "Uncultured" Cells and Organisms?; 5 Economic and Market Considerations; 5.1 The Pharmaceutical Market 5.2 Personal Care Products5.3 Chemicals, Industrial and Technical Enzymes; 5.4 Food, Dietary Supplements (Functional Food, Nutraceuticals), and Feed Products; 6 Conclusions; References; Part I: Suspension Culture of Bacteria, Yeasts, and Filamentous Fungi; 1 Bacterial Suspension Cultures; 1.1 Introduction; 1.2 Organisms, Cells, and their Products; 1.2.1 Bacteria as Production Platform for Various Products; 1.2.2 Historical Outline for Escherichia coli; 1.2.3 Industrial Aspects of Bacterial Expression Systems; 1.3 Bioprocess Design Aspects for Recombinant Products 1.3.1 Bacterial Cultivation Processes1.3.2 Gram Negative Cell Factory: Cellular Compartments and Transport across Membranes; 1.3.3 Industrial Strategies: Quality, Folding State, and Location of Recombinant Protein Products; 1.3.4 Approaches towards Bioprocess Design, Optimization, and Manufacturing; 1.3.5 Bacterial Bioprocess Design; 1.3.5.1 Technical and Physiological Constraints for Bacterial Bioprocess Design; 1.3.5.2 Media Design; 1.3.5.3 Product Titer is Determined by the Biomass Concentration and the Specific Productivity qp; 1.3.6 Industrial Production Strategy by Two-Step Cultivation 1.3.6.1 Batch Phase for the Accumulation of Biomass1.3.6.2 Structured Approach Towards Batch Design; 1.3.6.3 Fed-Batch Phase Process Design from Scratch; 1.3.6.4 Induction Phase: Product Formation Characteristics; 1.3.6.5 Process Parameters Impacting Recombinant Product Formation; 1.3.6.6 Concept of Time-Space Yield; 1.4 Basic Bioreactor Design Aspects; 1.4.1 Introduction; 1.4.2 Vessel Design and Construction; 1.4.3 Dimensioning; 1.4.3.1 Materials of Construction; 1.4.3.2 Surface Quality and Welding; 1.4.3.3 Nozzles and Ports; 1.4.4 Mass Transfer; 1.4.5 Cleaning in Place 1.4.6 Steaming in Place |
| Record Nr. | UNINA-9910132190303321 |
| Weinheim, Germany : , : Wiley-Blackwell, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Industrial scale suspension culture of living cells / / edited by Hans-Peter Meyer and Diego R. Schmidhalter
| Industrial scale suspension culture of living cells / / edited by Hans-Peter Meyer and Diego R. Schmidhalter |
| Pubbl/distr/stampa | Weinheim, Germany : , : Wiley-Blackwell, , 2014 |
| Descrizione fisica | 1 online resource (644 p.) |
| Disciplina |
611
611/.01816 |
| Soggetto topico |
Industrial microbiology - Methodology
Cell culture - Industrial applications Biotechnology |
| ISBN |
3-527-68335-6
3-527-68332-1 3-527-68334-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Industrial Scale Suspension Culture of Living Cells; Forword; Contents; Preface; List of Contributors; The History and Economic Relevance of Industrial Scale Suspension Culture of Living Cells; 1 Introduction; 2 A Short History of Suspension Culture (Fermentation); 2.1 Ethanol, Organic Acids, and Solvents, the Beginning; 2.2 Vitamins Fermentation Takes a Long Time to Develop; 2.3 Steroids, the First Large-Scale Biocatalysis Processes; 2.4 Antibiotics, a US-Lead Turning Point in Fermentation Technology; 2.5 Amino Acids, a Japanese Fermentation Success Story
2.6 Enzymes, a European Fermentation Success Story2.7 Single Cell Proteins, an Economic Flop; 2.8 Biofuels are Controversial Story; 2.9 Recombinant DNA Technology Based Products (Monoclonal Antibodies and Other Recombinant Proteins), Setting off an Avalanche of New Products; 3 The Contemporary Situation; 3.1 How Long Can the USA Keep its Leading Role?; 3.2 China and India Become Global Forces in Fermentation; 4 The Future of Suspension Culture; 4.1 New Frontiers; 4.2 Yet "Uncultured" Cells and Organisms?; 5 Economic and Market Considerations; 5.1 The Pharmaceutical Market 5.2 Personal Care Products5.3 Chemicals, Industrial and Technical Enzymes; 5.4 Food, Dietary Supplements (Functional Food, Nutraceuticals), and Feed Products; 6 Conclusions; References; Part I: Suspension Culture of Bacteria, Yeasts, and Filamentous Fungi; 1 Bacterial Suspension Cultures; 1.1 Introduction; 1.2 Organisms, Cells, and their Products; 1.2.1 Bacteria as Production Platform for Various Products; 1.2.2 Historical Outline for Escherichia coli; 1.2.3 Industrial Aspects of Bacterial Expression Systems; 1.3 Bioprocess Design Aspects for Recombinant Products 1.3.1 Bacterial Cultivation Processes1.3.2 Gram Negative Cell Factory: Cellular Compartments and Transport across Membranes; 1.3.3 Industrial Strategies: Quality, Folding State, and Location of Recombinant Protein Products; 1.3.4 Approaches towards Bioprocess Design, Optimization, and Manufacturing; 1.3.5 Bacterial Bioprocess Design; 1.3.5.1 Technical and Physiological Constraints for Bacterial Bioprocess Design; 1.3.5.2 Media Design; 1.3.5.3 Product Titer is Determined by the Biomass Concentration and the Specific Productivity qp; 1.3.6 Industrial Production Strategy by Two-Step Cultivation 1.3.6.1 Batch Phase for the Accumulation of Biomass1.3.6.2 Structured Approach Towards Batch Design; 1.3.6.3 Fed-Batch Phase Process Design from Scratch; 1.3.6.4 Induction Phase: Product Formation Characteristics; 1.3.6.5 Process Parameters Impacting Recombinant Product Formation; 1.3.6.6 Concept of Time-Space Yield; 1.4 Basic Bioreactor Design Aspects; 1.4.1 Introduction; 1.4.2 Vessel Design and Construction; 1.4.3 Dimensioning; 1.4.3.1 Materials of Construction; 1.4.3.2 Surface Quality and Welding; 1.4.3.3 Nozzles and Ports; 1.4.4 Mass Transfer; 1.4.5 Cleaning in Place 1.4.6 Steaming in Place |
| Record Nr. | UNINA-9910830860803321 |
| Weinheim, Germany : , : Wiley-Blackwell, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular polymorphism of man [[electronic resource] ] : structural and functional individual multiformity of biomacromolecules / / Sergei D. Varfolomyev and Gennady E. Zaikov, editors
| Molecular polymorphism of man [[electronic resource] ] : structural and functional individual multiformity of biomacromolecules / / Sergei D. Varfolomyev and Gennady E. Zaikov, editors |
| Pubbl/distr/stampa | New York, : Nova Science Publishers, c2011 |
| Descrizione fisica | 1 online resource (352 p.) |
| Disciplina | 611/.01816 |
| Altri autori (Persone) |
VarfolomeevSergeĭ Dmitrievich
ZaikovG. E <1935-> (Gennadiĭ Efremovich) |
| Collana | Genetics--research and issues |
| Soggetto topico |
Genetic polymorphisms
Phenotypic plasticity |
| Soggetto genere / forma | Electronic books. |
| ISBN | 1-61324-929-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Human enzymes : genetic, proteomic, and catalytic polymorphism / S.D. Varfolomeev, I.N. Kurochkin, and I.A. Gariev -- Polymorphism of tumor-suppressor genes and genetic control of carcinogenesis / M.M. Aslanyan ... [et al.] -- Association of candidate genes polymorphism with asthma in Bashkortostan republic of Russia / E.K. Khusnutdinova ... [et al.] -- Genes and languages : is there correlations between MTDNA data and geography of Altay and Ural languages / E. Khusnutdinova and I. Kutuev -- Common and special features of the human ribosomal DNA / Natalia S. Kupriyanova and Alexei P. Ryskov -- Ethnic genomics of the East European human populations / S.A. Limborska ... [et al.] -- Retroelement insertion polymorphism and modulation of human gene activity / I.Z. Mamedov ... [et al.] -- Biomedical aspects in investigations of biochemical polymorphism of actins and some actin-binding proteins / S.S. Shishkin ... [et al.] -- Molecular mechanisms of adaptation : stress and aggression / A.G. Tonevitsky ... [et al.] -- Ethnogenomics : the genetic history of humans written in chromosomal DNA markers / L.A. Zhivotovsky and E.K. Khusnutdinova. |
| Record Nr. | UNINA-9910456666803321 |
| New York, : Nova Science Publishers, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular polymorphism of man [[electronic resource] ] : structural and functional individual multiformity of biomacromolecules / / Sergei D. Varfolomyev and Gennady E. Zaikov, editors
| Molecular polymorphism of man [[electronic resource] ] : structural and functional individual multiformity of biomacromolecules / / Sergei D. Varfolomyev and Gennady E. Zaikov, editors |
| Pubbl/distr/stampa | New York, : Nova Science Publishers, c2011 |
| Descrizione fisica | 1 online resource (352 p.) |
| Disciplina | 611/.01816 |
| Altri autori (Persone) |
VarfolomeevSergeĭ Dmitrievich
ZaikovG. E <1935-> (Gennadiĭ Efremovich) |
| Collana | Genetics--research and issues |
| Soggetto topico |
Genetic polymorphisms
Phenotypic plasticity |
| ISBN | 1-61324-929-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Human enzymes : genetic, proteomic, and catalytic polymorphism / S.D. Varfolomeev, I.N. Kurochkin, and I.A. Gariev -- Polymorphism of tumor-suppressor genes and genetic control of carcinogenesis / M.M. Aslanyan ... [et al.] -- Association of candidate genes polymorphism with asthma in Bashkortostan republic of Russia / E.K. Khusnutdinova ... [et al.] -- Genes and languages : is there correlations between MTDNA data and geography of Altay and Ural languages / E. Khusnutdinova and I. Kutuev -- Common and special features of the human ribosomal DNA / Natalia S. Kupriyanova and Alexei P. Ryskov -- Ethnic genomics of the East European human populations / S.A. Limborska ... [et al.] -- Retroelement insertion polymorphism and modulation of human gene activity / I.Z. Mamedov ... [et al.] -- Biomedical aspects in investigations of biochemical polymorphism of actins and some actin-binding proteins / S.S. Shishkin ... [et al.] -- Molecular mechanisms of adaptation : stress and aggression / A.G. Tonevitsky ... [et al.] -- Ethnogenomics : the genetic history of humans written in chromosomal DNA markers / L.A. Zhivotovsky and E.K. Khusnutdinova. |
| Record Nr. | UNINA-9910781772103321 |
| New York, : Nova Science Publishers, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular polymorphism of man : structural and functional individual multiformity of biomacromolecules / / Sergei D. Varfolomyev and Gennady E. Zaikov, editors
| Molecular polymorphism of man : structural and functional individual multiformity of biomacromolecules / / Sergei D. Varfolomyev and Gennady E. Zaikov, editors |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | New York, : Nova Science Publishers, c2011 |
| Descrizione fisica | 1 online resource (352 p.) |
| Disciplina | 611/.01816 |
| Altri autori (Persone) |
VarfolomeevSergeĭ Dmitrievich
ZaikovG. E <1935-> (Gennadiĭ Efremovich) |
| Collana | Genetics--research and issues |
| Soggetto topico |
Genetic polymorphisms
Phenotypic plasticity |
| ISBN | 1-61324-929-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Human enzymes : genetic, proteomic, and catalytic polymorphism / S.D. Varfolomeev, I.N. Kurochkin, and I.A. Gariev -- Polymorphism of tumor-suppressor genes and genetic control of carcinogenesis / M.M. Aslanyan ... [et al.] -- Association of candidate genes polymorphism with asthma in Bashkortostan republic of Russia / E.K. Khusnutdinova ... [et al.] -- Genes and languages : is there correlations between MTDNA data and geography of Altay and Ural languages / E. Khusnutdinova and I. Kutuev -- Common and special features of the human ribosomal DNA / Natalia S. Kupriyanova and Alexei P. Ryskov -- Ethnic genomics of the East European human populations / S.A. Limborska ... [et al.] -- Retroelement insertion polymorphism and modulation of human gene activity / I.Z. Mamedov ... [et al.] -- Biomedical aspects in investigations of biochemical polymorphism of actins and some actin-binding proteins / S.S. Shishkin ... [et al.] -- Molecular mechanisms of adaptation : stress and aggression / A.G. Tonevitsky ... [et al.] -- Ethnogenomics : the genetic history of humans written in chromosomal DNA markers / L.A. Zhivotovsky and E.K. Khusnutdinova. |
| Record Nr. | UNINA-9910973151303321 |
| New York, : Nova Science Publishers, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
The X in sex [[electronic resource] ] : how the X chromosome controls our lives / / David Bainbridge
| The X in sex [[electronic resource] ] : how the X chromosome controls our lives / / David Bainbridge |
| Autore | Bainbridge David |
| Pubbl/distr/stampa | Cambridge, Mass., : Harvard University Press, 2003 |
| Descrizione fisica | 1 online resource (216 p.) |
| Disciplina | 611/.01816 |
| Soggetto topico |
X chromosome
Sex determination, Genetic |
| Soggetto genere / forma | Electronic books. |
| ISBN | 0-674-02041-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Frontmatter -- CONTENTS -- PROLOGUE -- 1 . MAKING A DIFFERENCE -- 2 . THE DUKE OF KENT’S TESTICLES -- 3 . THE DOUBLE LIFE OF WOMEN -- FURTHER READING -- GLOSSARY -- INDEX |
| Record Nr. | UNINA-9910455132603321 |
|
Bainbridge David
|
||
| Cambridge, Mass., : Harvard University Press, 2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
The X in sex [[electronic resource] ] : how the X chromosome controls our lives / / David Bainbridge
| The X in sex [[electronic resource] ] : how the X chromosome controls our lives / / David Bainbridge |
| Autore | Bainbridge David |
| Pubbl/distr/stampa | Cambridge, Mass., : Harvard University Press, 2003 |
| Descrizione fisica | 1 online resource (216 p.) |
| Disciplina | 611/.01816 |
| Soggetto topico |
X chromosome
Sex determination, Genetic |
| ISBN | 0-674-02041-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Frontmatter -- CONTENTS -- PROLOGUE -- 1 . MAKING A DIFFERENCE -- 2 . THE DUKE OF KENT’S TESTICLES -- 3 . THE DOUBLE LIFE OF WOMEN -- FURTHER READING -- GLOSSARY -- INDEX |
| Record Nr. | UNINA-9910778455303321 |
|
Bainbridge David
|
||
| Cambridge, Mass., : Harvard University Press, 2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
The X in sex : how the X chromosome controls our lives / / David Bainbridge
| The X in sex : how the X chromosome controls our lives / / David Bainbridge |
| Autore | Bainbridge David |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Cambridge, Mass., : Harvard University Press, 2003 |
| Descrizione fisica | 1 online resource (216 p.) |
| Disciplina | 611/.01816 |
| Soggetto topico |
X chromosome
Genetic sex determination |
| ISBN |
9780674020412
0674020413 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Frontmatter -- CONTENTS -- PROLOGUE -- 1 . MAKING A DIFFERENCE -- 2 . THE DUKE OF KENT’S TESTICLES -- 3 . THE DOUBLE LIFE OF WOMEN -- FURTHER READING -- GLOSSARY -- INDEX |
| Record Nr. | UNINA-9910959596103321 |
|
Bainbridge David
|
||
| Cambridge, Mass., : Harvard University Press, 2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||