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Antimicrobial Resistance : A One Health Perspective
| Antimicrobial Resistance : A One Health Perspective |
| Autore | Mareș Mihai |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | London : , : IntechOpen, , 2021 |
| Descrizione fisica | 1 online resource (210 pages) |
| Disciplina | 579.165 |
| Altri autori (Persone) |
ErinSwee Hua
LaiKok-Song CristinaRomeo-Teodor |
| Soggetto topico | Pathogenic microorganisms |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Altri titoli varianti | Antimicrobial Resistance |
| Record Nr. | UNINA-9910688273003321 |
Mareș Mihai
|
||
| London : , : IntechOpen, , 2021 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
BSL3 and BSL4 agents [[electronic resource] ] : epidemiology, microbiology, and practical guidelines / / edited by Mandy C. Elschner ... [et al.]
| BSL3 and BSL4 agents [[electronic resource] ] : epidemiology, microbiology, and practical guidelines / / edited by Mandy C. Elschner ... [et al.] |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Weinheim, Germany, : Wiley-Blackwell, c2012 |
| Descrizione fisica | 1 online resource (401 p.) |
| Disciplina |
579.165
599.0292 599/.0292 |
| Altri autori (Persone) | ElschnerMandy C |
| Soggetto topico |
Pathogenic microorganisms - Analysis
Pathogenic microorganisms - Detection Bacterial diseases - Epidemiology Bacterial diseases - Microbiology Molecular epidemiology Biotechnology - Safety measures Biological decontamination |
| ISBN |
3-527-64509-8
1-280-78270-6 9786613693099 3-527-64510-1 3-527-64511-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt. A. Pathogens -- pt. B. Practical guidelines. |
| Record Nr. | UNINA-9910137629303321 |
| Weinheim, Germany, : Wiley-Blackwell, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
BSL3 and BSL4 agents : epidemiology, microbiology, and practical guidelines / / edited by Mandy C. Elschner ... [et al.]
| BSL3 and BSL4 agents : epidemiology, microbiology, and practical guidelines / / edited by Mandy C. Elschner ... [et al.] |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Weinheim, Germany, : Wiley-Blackwell, c2012 |
| Descrizione fisica | 1 online resource (401 p.) |
| Disciplina |
579.165
599.0292 599/.0292 |
| Altri autori (Persone) | ElschnerMandy C |
| Soggetto topico |
Pathogenic microorganisms - Analysis
Pathogenic microorganisms - Detection Bacterial diseases - Epidemiology Bacterial diseases - Microbiology Molecular epidemiology Biotechnology - Safety measures Biological decontamination |
| ISBN |
9786613693099
9783527645091 3527645098 9781280782701 1280782706 9783527645107 3527645101 9783527645114 352764511X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt. A. Pathogens -- pt. B. Practical guidelines. |
| Record Nr. | UNINA-9910821610303321 |
| Weinheim, Germany, : Wiley-Blackwell, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
BSL3 and BSL4 agents [[electronic resource] ] : proteomics, glycomics, and antigenicity / / edited by Jiri Stulik ... [et al.]
| BSL3 and BSL4 agents [[electronic resource] ] : proteomics, glycomics, and antigenicity / / edited by Jiri Stulik ... [et al.] |
| Pubbl/distr/stampa | Weinheim, : Wiley-Blackwell, c2011 |
| Descrizione fisica | 1 online resource (258 p.) |
| Disciplina | 579.165 |
| Altri autori (Persone) | ŠtulikJ̌irí |
| Soggetto topico |
Pathogenic microorganisms - Analysis
Proteomics Glycomics Antigens |
| ISBN |
1-283-83535-5
3-527-63820-2 3-527-63821-0 3-527-63819-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
BSL3 and BSL4 Agents: Proteomics, Glycomics, and Antigenicity; Contents; Preface; List of Contributors; 1: Introduction: Application of Proteomic Technologies for the Analysis of Microbial Infections; 1.1 Introduction; 1.2 Search for New Factors of Virulence and Potential Diagnostic Markers; 1.3 Search for New Vaccine Candidates; 1.4 Analysis of Post-Translational Modifications of Bacterial Proteins and Protein-Protein Interactions; 1.5 Conclusions; References; Part One: Basic Proteomic Methods; 2: Separation of Proteins and Peptides; 2.1 Introduction; 2.1.1 Gel-Based Separation
2.1.1.1 One-Dimensional Electrophoresis2.1.1.2 Two-Dimensional Electrophoresis; 2.1.1.3 Protein Staining and Image Analysis; 2.1.1.4 2-DE Limitations; 2.1.2 In Solution-"Gel Free" Proteomics; 2.1.3 Column Chromatography; 2.1.3.1 Size Exclusion Chromatography; 2.1.3.2 Reversed-Phase Liquid Chromatography; 2.1.3.3 Hydrophilic Interaction Liquid Chromatography; 2.1.3.4 Ion Exchanger Chromatography; 2.1.3.5 Affinity Chromatography; 2.1.3.6 Multidimensional Chromatography; 2.1.4 Liquid Phase IEF and Electrophoresis; 2.1.5 Alternative Separation Technologies; Acknowledgment; References 3: Basic Mass Spectrometric Approaches3.1 Introduction; 3.2 Ionization; 3.2.1 Matrix-Assisted Laser Desorption/Ionization; 3.2.2 Electrospray Ionization; 3.3 Mass Analyzers; 3.3.1 Time of Flight; 3.3.2 Reflectron TOF; 3.3.3 Quadrupole and Ion Trap; 3.3.4 Fourier Transformation Ion Cyclotron; 3.3.5 Tandem Mass Analyzers; 3.3.6 Ion Detection; 3.4 Protein Identification; 3.4.1 Combination of 2-DE and MS; 3.4.2 Peptide Mass Fingerprinting; 3.4.3 Peptide Sequencing (PMF); 3.4.4 Shotgun Proteomics; 3.5 Conclusion; Acknowledgments; References; 4: Quantitative Mass Spectrometric Approaches 4.1 Introduction4.1.1 Gel-Based Quantitative Proteomic Methods; 4.1.2 Shotgun Quantitative Proteomic Methods; 4.1.3 Labeling Methods; 4.1.3.1 Metabolic Incorporation of Stable Isotopes; 4.1.3.2 Enzymatic Incorporation of Stable Isotopes; 4.1.3.3 Chemical Incorporation of Stable Isotopes; 4.2 iTRAQ Analysis of Bacterial Pathogens; 4.2.1 Bacterial Cell Disruption and Protein Extraction; 4.2.2 Determination of Protein Concentration; 4.2.3 Protein Digestion; 4.2.4 Peptide Labeling with iTRAQ Tags; 4.2.5 Protocol for iTRAQ Analysis of Bacterial Proteins; References 5: BN-PAGE of Microbial Protein Complexes5.1 Introduction; 5.2 Methods for Studying Protein-Protein Interactions; 5.3 Blue Native Polyacrylamide Gel Electophoresis; 5.3.1 Sample Preparation; 5.3.1.1 Non-Denaturing Conditions; 5.3.1.2 Selection of Detergent and Its Optimal Concentration; 5.3.1.3 Membrane and Cytosolic Fraction Separation; 5.3.2 1D BN-PAGE; 5.3.3 2D BN/SDS-PAGE; 5.4 Evaluation of BN-PAGE-Staining, MS, Western Blotting; 5.4.1 Staining; 5.4.1.1 Silver Staining; 5.4.1.2 Fluorescent Staining; 5.4.1.3 Coomassie Staining; 5.4.2 Mass Spectrometry; 5.4.3 Western Blotting 5.4.4 Other Methods of Visualization |
| Record Nr. | UNINA-9910133840603321 |
| Weinheim, : Wiley-Blackwell, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
BSL3 and BSL4 agents : proteomics, glycomics, and antigenicity / / edited by Jiri Stulik ... [et al.]
| BSL3 and BSL4 agents : proteomics, glycomics, and antigenicity / / edited by Jiri Stulik ... [et al.] |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Weinheim, : Wiley-Blackwell, c2011 |
| Descrizione fisica | 1 online resource (258 p.) |
| Disciplina | 579.165 |
| Altri autori (Persone) | StulikJiri |
| Soggetto topico |
Pathogenic microorganisms - Analysis
Proteomics Glycomics Antigens |
| ISBN |
9781283835350
1283835355 9783527638208 3527638202 9783527638215 3527638210 9783527638192 3527638199 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
BSL3 and BSL4 Agents: Proteomics, Glycomics, and Antigenicity; Contents; Preface; List of Contributors; 1: Introduction: Application of Proteomic Technologies for the Analysis of Microbial Infections; 1.1 Introduction; 1.2 Search for New Factors of Virulence and Potential Diagnostic Markers; 1.3 Search for New Vaccine Candidates; 1.4 Analysis of Post-Translational Modifications of Bacterial Proteins and Protein-Protein Interactions; 1.5 Conclusions; References; Part One: Basic Proteomic Methods; 2: Separation of Proteins and Peptides; 2.1 Introduction; 2.1.1 Gel-Based Separation
2.1.1.1 One-Dimensional Electrophoresis2.1.1.2 Two-Dimensional Electrophoresis; 2.1.1.3 Protein Staining and Image Analysis; 2.1.1.4 2-DE Limitations; 2.1.2 In Solution-"Gel Free" Proteomics; 2.1.3 Column Chromatography; 2.1.3.1 Size Exclusion Chromatography; 2.1.3.2 Reversed-Phase Liquid Chromatography; 2.1.3.3 Hydrophilic Interaction Liquid Chromatography; 2.1.3.4 Ion Exchanger Chromatography; 2.1.3.5 Affinity Chromatography; 2.1.3.6 Multidimensional Chromatography; 2.1.4 Liquid Phase IEF and Electrophoresis; 2.1.5 Alternative Separation Technologies; Acknowledgment; References 3: Basic Mass Spectrometric Approaches3.1 Introduction; 3.2 Ionization; 3.2.1 Matrix-Assisted Laser Desorption/Ionization; 3.2.2 Electrospray Ionization; 3.3 Mass Analyzers; 3.3.1 Time of Flight; 3.3.2 Reflectron TOF; 3.3.3 Quadrupole and Ion Trap; 3.3.4 Fourier Transformation Ion Cyclotron; 3.3.5 Tandem Mass Analyzers; 3.3.6 Ion Detection; 3.4 Protein Identification; 3.4.1 Combination of 2-DE and MS; 3.4.2 Peptide Mass Fingerprinting; 3.4.3 Peptide Sequencing (PMF); 3.4.4 Shotgun Proteomics; 3.5 Conclusion; Acknowledgments; References; 4: Quantitative Mass Spectrometric Approaches 4.1 Introduction4.1.1 Gel-Based Quantitative Proteomic Methods; 4.1.2 Shotgun Quantitative Proteomic Methods; 4.1.3 Labeling Methods; 4.1.3.1 Metabolic Incorporation of Stable Isotopes; 4.1.3.2 Enzymatic Incorporation of Stable Isotopes; 4.1.3.3 Chemical Incorporation of Stable Isotopes; 4.2 iTRAQ Analysis of Bacterial Pathogens; 4.2.1 Bacterial Cell Disruption and Protein Extraction; 4.2.2 Determination of Protein Concentration; 4.2.3 Protein Digestion; 4.2.4 Peptide Labeling with iTRAQ Tags; 4.2.5 Protocol for iTRAQ Analysis of Bacterial Proteins; References 5: BN-PAGE of Microbial Protein Complexes5.1 Introduction; 5.2 Methods for Studying Protein-Protein Interactions; 5.3 Blue Native Polyacrylamide Gel Electophoresis; 5.3.1 Sample Preparation; 5.3.1.1 Non-Denaturing Conditions; 5.3.1.2 Selection of Detergent and Its Optimal Concentration; 5.3.1.3 Membrane and Cytosolic Fraction Separation; 5.3.2 1D BN-PAGE; 5.3.3 2D BN/SDS-PAGE; 5.4 Evaluation of BN-PAGE-Staining, MS, Western Blotting; 5.4.1 Staining; 5.4.1.1 Silver Staining; 5.4.1.2 Fluorescent Staining; 5.4.1.3 Coomassie Staining; 5.4.2 Mass Spectrometry; 5.4.3 Western Blotting 5.4.4 Other Methods of Visualization |
| Record Nr. | UNINA-9910828394903321 |
| Weinheim, : Wiley-Blackwell, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
A Clinician's Dictionary of Pathogenic Microorganisms / / James H. Jorgensen
| A Clinician's Dictionary of Pathogenic Microorganisms / / James H. Jorgensen |
| Autore | Jorgensen James H. |
| Pubbl/distr/stampa | Washington, D.C. : , : John Wiley & Sons, Inc., , 2014 |
| Descrizione fisica | 1 online resource (317 pages) |
| Disciplina | 579.165 |
| Soggetto topico | Pathogenic microorganisms |
| ISBN | 1-68367-214-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910830184003321 |
|
Jorgensen James H.
|
||
| Washington, D.C. : , : John Wiley & Sons, Inc., , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Comprehensive analysis of parasite biology : from metabolism to drug discovery / / edited by Sylke Müller, Rachel Cerdan, and Ovidiu Radulescu
| Comprehensive analysis of parasite biology : from metabolism to drug discovery / / edited by Sylke Müller, Rachel Cerdan, and Ovidiu Radulescu |
| Pubbl/distr/stampa | Weinheim, Germany : , : Wiley-VCH Verlag GmbH & Co. KgaA, , 2016 |
| Descrizione fisica | 1 online resource (643 p.) |
| Disciplina | 579.165 |
| Collana | Drug Discovery in Infectious Diseases |
| Soggetto topico |
Pathogenic microorganisms
Parasitic diseases - Immunological aspects |
| ISBN |
3-527-69409-9
3-527-69411-0 3-527-69408-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910134853903321 |
| Weinheim, Germany : , : Wiley-VCH Verlag GmbH & Co. KgaA, , 2016 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Comprehensive analysis of parasite biology : from metabolism to drug discovery / / edited by Sylke Müller, Rachel Cerdan, and Ovidiu Radulescu
| Comprehensive analysis of parasite biology : from metabolism to drug discovery / / edited by Sylke Müller, Rachel Cerdan, and Ovidiu Radulescu |
| Pubbl/distr/stampa | Weinheim, Germany : , : Wiley-VCH Verlag GmbH & Co. KgaA, , 2016 |
| Descrizione fisica | 1 online resource (643 p.) |
| Disciplina | 579.165 |
| Collana | Drug Discovery in Infectious Diseases |
| Soggetto topico |
Pathogenic microorganisms
Parasitic diseases - Immunological aspects |
| ISBN |
3-527-69409-9
3-527-69411-0 3-527-69408-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910830556003321 |
| Weinheim, Germany : , : Wiley-VCH Verlag GmbH & Co. KgaA, , 2016 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel
| Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, 2009 |
| Descrizione fisica | 1 online resource (193 p.) |
| Disciplina | 579.165 |
| Altri autori (Persone) |
KosticTanja
ButayePatrick SchrenzelJacques |
| Soggetto topico |
Pathogenic microorganisms - Detection
DNA microarrays |
| ISBN |
1-282-29205-6
9786612292057 3-527-62668-9 3-527-62669-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Detection of Highly Dangerous Pathogens: Microarray Methods for the Detection of BSL 3 and BSL 4 Agents; Contents; This Publication is Supported by COST; Preface; List of Contributors; 1 Introduction to Microarray-Based Detection Methods; 1.1 Introduction to Microarray Technology; 1.2 Technical Aspects of Microarray Technology; 1.2.1 Probes; 1.2.1.1 Genome Fragments; 1.2.1.2 PCR Products; 1.2.1.3 Oligonucleotide Probes; 1.2.2 Substrates for Printing; 1.2.2.1 Slides with Poly-L-lysine Coating; 1.2.2.2 Slides with Amino Silane Coating; 1.2.2.3 Slides with Aldehyde Coating
1.2.2.4 Slides with Epoxy Coating1.2.2.5 Proprietary Surface Chemistries; 1.2.2.6 Probe Spacers; 1.2.3 Targets for Microarray Analysis; 1.2.3.1 Target Amplifications and Sensitivity Issues; 1.2.3.2 Labeling of the Targets; 1.2.3.3 Hybridization and Wash Conditions; 1.2.4 Classical Commercially Available Microarray Formats; 1.2.4.1 Spotting Approaches; 1.2.4.2 In Situ Synthesis; 1.2.5 Alternative Methods for Improving Microarray-Based Detection Sensitivity; 1.2.5.1 Resonance-Light Scattering (RLS); 1.2.5.2 Planar-Waveguide Technology (PWT); 1.2.5.3 Liquid Arrays 1.2.5.4 Three-Dimensional Microarray Formats1.2.6 Marker Genes Used on MDMs; 1.3 Analysis and Quality Control Aspects; 1.4 Applications of Microarray Technology in Microbial Diagnostics; 1.4.1 Gene Expression Studies; 1.4.2 Comparative Genomic Hybridization (CGH); 1.4.3 Generic or Universal Microarrays; 1.4.4 Microarrays for Sequence Analysis; 1.4.5 Microbial Diagnostic Microarrays (MDMs); 1.5 Further Developments and New Perspectives Regarding Array Sensitivity and Specificity; 1.6 Conclusions; References; Part I: Methods; 2 Long Oligonucleotide Microarray-Based Microbial Detection 2.1 Introduction2.2 Method; 2.2.1 DNA Extraction; 2.2.2 Ф29 Amplification; 2.2.3 Klenow Amplification/Labeling; 2.2.4 Probe and Slide Preparation; 2.2.5 Slide Processing Protocol (for Amino Surfaces); 2.2.6 Hybridization and Slide Washing; 2.2.7 Comments; 2.3 Our Test System and Results; 2.4 Conclusions; References; 3 Sequence-Specific End-Labeling of Oligonucleotides; 3.1 Introduction; 3.2 Probe Design; 3.3 Slide Preparation (Spotting); 3.4 Slide Processing Protocol ( for Aldehyde Surfaces); 3.5 DNA Extraction and PCR Amplification of the Targeted Gene 3.6 Shrimp Alkaline Phosphatase Treatment3.7 Labeling; 3.8 Hybridization and Slide Washing; 3.9 Data Analysis; 3.10 Costs; 3.11 Microarray for Detection of Pathogenic Bacteria; References; 4 Non-Cognate Approaches for Pathogen Detection on Microarrays; 4.1 Introduction; 4.2 Non-Cognate Hybridization System; 4.2.1 Concept; 4.2.2 Definition of the Optimal Probe Length; 4.2.3 Virtual Assessment of Array Performances (in Silico Experiments); 4.2.4 Array Manufacturing and Hybridization (Wet-Lab Experiments); 4.2.5 Analysis; 4.3 Perspectives; References; 5 Patterning Techniques for Array Platforms 5.1 Introduction |
| Record Nr. | UNINA-9910143129503321 |
| Weinheim, : Wiley-VCH, 2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel
| Detection of highly dangerous pathogens [[electronic resource] ] : microarray methods for BSL 3 and BSL 4 agents / / edited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, 2009 |
| Descrizione fisica | 1 online resource (193 p.) |
| Disciplina | 579.165 |
| Altri autori (Persone) |
KosticTanja
ButayePatrick SchrenzelJacques |
| Soggetto topico |
Pathogenic microorganisms - Detection
DNA microarrays |
| ISBN |
1-282-29205-6
9786612292057 3-527-62668-9 3-527-62669-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Detection of Highly Dangerous Pathogens: Microarray Methods for the Detection of BSL 3 and BSL 4 Agents; Contents; This Publication is Supported by COST; Preface; List of Contributors; 1 Introduction to Microarray-Based Detection Methods; 1.1 Introduction to Microarray Technology; 1.2 Technical Aspects of Microarray Technology; 1.2.1 Probes; 1.2.1.1 Genome Fragments; 1.2.1.2 PCR Products; 1.2.1.3 Oligonucleotide Probes; 1.2.2 Substrates for Printing; 1.2.2.1 Slides with Poly-L-lysine Coating; 1.2.2.2 Slides with Amino Silane Coating; 1.2.2.3 Slides with Aldehyde Coating
1.2.2.4 Slides with Epoxy Coating1.2.2.5 Proprietary Surface Chemistries; 1.2.2.6 Probe Spacers; 1.2.3 Targets for Microarray Analysis; 1.2.3.1 Target Amplifications and Sensitivity Issues; 1.2.3.2 Labeling of the Targets; 1.2.3.3 Hybridization and Wash Conditions; 1.2.4 Classical Commercially Available Microarray Formats; 1.2.4.1 Spotting Approaches; 1.2.4.2 In Situ Synthesis; 1.2.5 Alternative Methods for Improving Microarray-Based Detection Sensitivity; 1.2.5.1 Resonance-Light Scattering (RLS); 1.2.5.2 Planar-Waveguide Technology (PWT); 1.2.5.3 Liquid Arrays 1.2.5.4 Three-Dimensional Microarray Formats1.2.6 Marker Genes Used on MDMs; 1.3 Analysis and Quality Control Aspects; 1.4 Applications of Microarray Technology in Microbial Diagnostics; 1.4.1 Gene Expression Studies; 1.4.2 Comparative Genomic Hybridization (CGH); 1.4.3 Generic or Universal Microarrays; 1.4.4 Microarrays for Sequence Analysis; 1.4.5 Microbial Diagnostic Microarrays (MDMs); 1.5 Further Developments and New Perspectives Regarding Array Sensitivity and Specificity; 1.6 Conclusions; References; Part I: Methods; 2 Long Oligonucleotide Microarray-Based Microbial Detection 2.1 Introduction2.2 Method; 2.2.1 DNA Extraction; 2.2.2 Ф29 Amplification; 2.2.3 Klenow Amplification/Labeling; 2.2.4 Probe and Slide Preparation; 2.2.5 Slide Processing Protocol (for Amino Surfaces); 2.2.6 Hybridization and Slide Washing; 2.2.7 Comments; 2.3 Our Test System and Results; 2.4 Conclusions; References; 3 Sequence-Specific End-Labeling of Oligonucleotides; 3.1 Introduction; 3.2 Probe Design; 3.3 Slide Preparation (Spotting); 3.4 Slide Processing Protocol ( for Aldehyde Surfaces); 3.5 DNA Extraction and PCR Amplification of the Targeted Gene 3.6 Shrimp Alkaline Phosphatase Treatment3.7 Labeling; 3.8 Hybridization and Slide Washing; 3.9 Data Analysis; 3.10 Costs; 3.11 Microarray for Detection of Pathogenic Bacteria; References; 4 Non-Cognate Approaches for Pathogen Detection on Microarrays; 4.1 Introduction; 4.2 Non-Cognate Hybridization System; 4.2.1 Concept; 4.2.2 Definition of the Optimal Probe Length; 4.2.3 Virtual Assessment of Array Performances (in Silico Experiments); 4.2.4 Array Manufacturing and Hybridization (Wet-Lab Experiments); 4.2.5 Analysis; 4.3 Perspectives; References; 5 Patterning Techniques for Array Platforms 5.1 Introduction |
| Record Nr. | UNINA-9910831037603321 |
| Weinheim, : Wiley-VCH, 2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
