Info
- Utilizzare la checkbox di selezione a fianco di ciascun documento per attivare le funzionalità di stampa, invio email, download nei formati disponibili del (i) record.
Molecular biology of RNA : new perspectives / / edited by Masayori Inouye, Bernard S. Dudock
| Molecular biology of RNA : new perspectives / / edited by Masayori Inouye, Bernard S. Dudock |
| Pubbl/distr/stampa | San Diego : , : Academic Press, , [1987] |
| Descrizione fisica | 1 online resource (464 p.) |
| Disciplina | 574.87/3283 |
| Soggetto topico |
RNA
Molecular biology Molecular genetics |
| ISBN | 1-4832-7390-3 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Biology of RNA: New Perspectives; Copyright Page; Table of Contents; Preface; PART I: RNA as an Enzyme; Chapter 1. Cleavage of RNA by RNAse P from Escherichia coli; I. Introduction; II. Aspects of the RNase P Reaction; III. Studies of Enzyme-Substrate Interactions; IV. Structure-Function Relationships in Ml RNA; V. Studies of the Protein Subunit of RNase P; VI. Hybrid Enzymes; VII. Conclusion; References; Chapter 2. Bacillus subtilis RNase P; I. The RNase P Components; II. Mechanism of RNase P Cleavage; III. Toward the Higher-Order Structure of RNase P RNA
IV. Structure-Function Relationships in the RNase P RNAV. Why Is the Catalytic Element of RNase P Composed of RNA?; References; Chapter 3. Multiple Enzymatic Activities of an Intervening Sequence RNA from Tetrahymena; I. Introduction; II. Self-Splicing RNA; III. The IVS RNA Enzyme; References; Chapter 4. Processing and Genetic Characterization of Self-Splicing RNAs of Bacteriophage T4; I. Introduction; II. Group I Splicing Mechanism for T4 td RNA; III. Nondirected Mutagenesis and Delineation of Two Functional Domains for Splicing in the td Intron; IV. Multiple Self-Splicing Introns in T4 V. ConclusionsReferences; PART II: RNA Splicing; Chapter 5. The Mammalian Pre-Messenger RNA Splicing Apparatus: A Ribosome in Pieces?; I. Introduction; II. The Discovery of snRNPs; III. The snRNPs-and-Splicing Hypothesis; IV. snRNP Components and Structure; V. Is Eukaryotic RNase P an Sm snRNP?; VI. Ul snRNPs Bind 5' Splice Sites; VII. U2, U5, and U4/U6 snRNPs Also Participate in Splicing; VIII. The Spliceosome-Ribosome Analogy; References; Chapter 6. Exon Sequences and Splice Site Proximity Play a Role in Splice Site Selection; I. Introduction II. Exon Sequences and Splice Site Proximity Play a Role in Splice Site SelectionIII. The Pattern of Splice Site Selection Is Altered in Different Extract Preparations and in Diluted Extracts; IV. Splice Site Selection Can Be Altered by Competition in Trans; V. Discussion; References; Chapter 7. Factors That Influence Alternative Splice Site Selection in Vitro; I. Introduction; II. Materials and Methods; III. Results; IV. Discussion; References; Chapter 8. Messenger RNA Splicing in Yeast; I. An Overview of Nuclear mRNA Splicing II. Preliminary in Vitro and in Vivo Characterization of Yeast mRNA SplicingIII. Characterization of Mutations in the Splicing Process; IV. The RNA Gene Products and the Spliceosome; V. Speculation; References; Chapter 9. Architecture of Fungal Introns: Implications for Spliceosome Assembly; I. Introduction; II. Branch Site-3' Splice Junction Relationship; III. Branch Site-5' Splice Junction Relationship; IV. Perspectives; References; Chapter 10. RNA Joining and Trypanosome Gene Expression; I. Introduction; II. Materials and Methods; III. Results; IV. Discussion; V. Summary; References PART III: RNA Viruses |
| Record Nr. | UNINA-9910789454303321 |
| San Diego : , : Academic Press, , [1987] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology of RNA : new perspectives / / edited by Masayori Inouye, Bernard S. Dudock
| Molecular biology of RNA : new perspectives / / edited by Masayori Inouye, Bernard S. Dudock |
| Pubbl/distr/stampa | San Diego : , : Academic Press, , [1987] |
| Descrizione fisica | 1 online resource (464 p.) |
| Disciplina | 574.87/3283 |
| Soggetto topico |
RNA
Molecular biology Molecular genetics |
| ISBN | 1-4832-7390-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Biology of RNA: New Perspectives; Copyright Page; Table of Contents; Preface; PART I: RNA as an Enzyme; Chapter 1. Cleavage of RNA by RNAse P from Escherichia coli; I. Introduction; II. Aspects of the RNase P Reaction; III. Studies of Enzyme-Substrate Interactions; IV. Structure-Function Relationships in Ml RNA; V. Studies of the Protein Subunit of RNase P; VI. Hybrid Enzymes; VII. Conclusion; References; Chapter 2. Bacillus subtilis RNase P; I. The RNase P Components; II. Mechanism of RNase P Cleavage; III. Toward the Higher-Order Structure of RNase P RNA
IV. Structure-Function Relationships in the RNase P RNAV. Why Is the Catalytic Element of RNase P Composed of RNA?; References; Chapter 3. Multiple Enzymatic Activities of an Intervening Sequence RNA from Tetrahymena; I. Introduction; II. Self-Splicing RNA; III. The IVS RNA Enzyme; References; Chapter 4. Processing and Genetic Characterization of Self-Splicing RNAs of Bacteriophage T4; I. Introduction; II. Group I Splicing Mechanism for T4 td RNA; III. Nondirected Mutagenesis and Delineation of Two Functional Domains for Splicing in the td Intron; IV. Multiple Self-Splicing Introns in T4 V. ConclusionsReferences; PART II: RNA Splicing; Chapter 5. The Mammalian Pre-Messenger RNA Splicing Apparatus: A Ribosome in Pieces?; I. Introduction; II. The Discovery of snRNPs; III. The snRNPs-and-Splicing Hypothesis; IV. snRNP Components and Structure; V. Is Eukaryotic RNase P an Sm snRNP?; VI. Ul snRNPs Bind 5' Splice Sites; VII. U2, U5, and U4/U6 snRNPs Also Participate in Splicing; VIII. The Spliceosome-Ribosome Analogy; References; Chapter 6. Exon Sequences and Splice Site Proximity Play a Role in Splice Site Selection; I. Introduction II. Exon Sequences and Splice Site Proximity Play a Role in Splice Site SelectionIII. The Pattern of Splice Site Selection Is Altered in Different Extract Preparations and in Diluted Extracts; IV. Splice Site Selection Can Be Altered by Competition in Trans; V. Discussion; References; Chapter 7. Factors That Influence Alternative Splice Site Selection in Vitro; I. Introduction; II. Materials and Methods; III. Results; IV. Discussion; References; Chapter 8. Messenger RNA Splicing in Yeast; I. An Overview of Nuclear mRNA Splicing II. Preliminary in Vitro and in Vivo Characterization of Yeast mRNA SplicingIII. Characterization of Mutations in the Splicing Process; IV. The RNA Gene Products and the Spliceosome; V. Speculation; References; Chapter 9. Architecture of Fungal Introns: Implications for Spliceosome Assembly; I. Introduction; II. Branch Site-3' Splice Junction Relationship; III. Branch Site-5' Splice Junction Relationship; IV. Perspectives; References; Chapter 10. RNA Joining and Trypanosome Gene Expression; I. Introduction; II. Materials and Methods; III. Results; IV. Discussion; V. Summary; References PART III: RNA Viruses |
| Record Nr. | UNINA-9910807753803321 |
| San Diego : , : Academic Press, , [1987] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
| RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby |
| Autore | Gjerde Douglas T |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (209 p.) |
| Disciplina |
572.88
572.8836 574.87/3283 |
| Altri autori (Persone) |
HornbyDavid, Dr.
HoangLee |
| Soggetto topico |
RNA - Analysis
RNA - Purification |
| ISBN |
1-282-68979-7
9786612689796 3-527-62719-7 3-527-62720-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography
1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism 2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction 3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection 5.3 RNA Chromatography Conditions |
| Record Nr. | UNINA-9910139923703321 |
Gjerde Douglas T
|
||
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
| RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby |
| Autore | Gjerde Douglas T |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (209 p.) |
| Disciplina |
572.88
572.8836 574.87/3283 |
| Altri autori (Persone) |
HornbyDavid, Dr.
HoangLee |
| Soggetto topico |
RNA - Analysis
RNA - Purification |
| ISBN |
1-282-68979-7
9786612689796 3-527-62719-7 3-527-62720-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography
1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism 2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction 3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection 5.3 RNA Chromatography Conditions |
| Record Nr. | UNINA-9910829855103321 |
|
Gjerde Douglas T
|
||
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
RNA purification and analysis : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
| RNA purification and analysis : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby |
| Autore | Gjerde Douglas T |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (209 p.) |
| Disciplina |
572.88
572.8836 574.87/3283 |
| Altri autori (Persone) |
HornbyDavid, Dr.
HoangLee |
| Soggetto topico |
RNA - Analysis
RNA - Purification |
| ISBN |
9786612689796
9781282689794 1282689797 9783527627196 3527627197 9783527627202 3527627200 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography
1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism 2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction 3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection 5.3 RNA Chromatography Conditions |
| Record Nr. | UNINA-9911018933803321 |
|
Gjerde Douglas T
|
||
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||