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RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
Autore Gjerde Douglas T
Pubbl/distr/stampa Weinheim, : Wiley-VCH
Descrizione fisica 1 online resource (209 p.)
Disciplina 572.88
572.8836
574.87/3283
Altri autori (Persone) HornbyDavid, Dr.
HoangLee
Soggetto topico RNA - Analysis
RNA - Purification
ISBN 1-282-68979-7
9786612689796
3-527-62719-7
3-527-62720-0
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography
1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism
2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction
3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration
References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection
5.3 RNA Chromatography Conditions
Record Nr. UNINA-9910139923703321
Gjerde Douglas T  
Weinheim, : Wiley-VCH
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
RNA purification and analysis [[electronic resource] ] : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
Autore Gjerde Douglas T
Pubbl/distr/stampa Weinheim, : Wiley-VCH
Descrizione fisica 1 online resource (209 p.)
Disciplina 572.88
572.8836
574.87/3283
Altri autori (Persone) HornbyDavid, Dr.
HoangLee
Soggetto topico RNA - Analysis
RNA - Purification
ISBN 1-282-68979-7
9786612689796
3-527-62719-7
3-527-62720-0
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography
1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism
2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction
3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration
References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection
5.3 RNA Chromatography Conditions
Record Nr. UNINA-9910829855103321
Gjerde Douglas T  
Weinheim, : Wiley-VCH
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
RNA purification and analysis : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
RNA purification and analysis : sample preparation, extraction, chromatography / / by Douglas T. Gjerde, Lee Hoang and David Hornby
Autore Gjerde Douglas T
Pubbl/distr/stampa Weinheim, : Wiley-VCH
Descrizione fisica 1 online resource (209 p.)
Disciplina 572.88
572.8836
574.87/3283
Altri autori (Persone) HornbyDavid, Dr.
HoangLee
Soggetto topico RNA - Analysis
RNA - Purification
ISBN 1-282-68979-7
9786612689796
3-527-62719-7
3-527-62720-0
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography
1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism
2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction
3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration
References4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection
5.3 RNA Chromatography Conditions
Record Nr. UNINA-9910876677603321
Gjerde Douglas T  
Weinheim, : Wiley-VCH
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui