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Alternative pre-mRNA splicing [[electronic resource] ] : theory and protocols / / edited by Stefan Stamm, Chris Smith, and Reinhard Lührmann
| Alternative pre-mRNA splicing [[electronic resource] ] : theory and protocols / / edited by Stefan Stamm, Chris Smith, and Reinhard Lührmann |
| Pubbl/distr/stampa | Weinheim, Germany, : Wiley-Blackwell, c2012 |
| Descrizione fisica | 1 online resource (662 p.) |
| Disciplina | 572.88 |
| Altri autori (Persone) |
StammStefan
SmithChris LührmannReinhard |
| Soggetto topico | Messenger RNA |
| ISBN |
3-527-64798-8
1-283-43213-7 9786613432131 3-527-63677-3 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt. 1. Theory -- pt. 2. Basic methods -- pt. 3. Detection of splicing events -- pt. 4. Analysis of splicing in vitro -- pt. 5. Analysis of splicing in vivo -- pt. 6. Manipulation of splicing events -- pt. 7. Bioinformatic analysis of splicing. |
| Record Nr. | UNINA-9910139724303321 |
| Weinheim, Germany, : Wiley-Blackwell, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Alternative pre-mRNA splicing : theory and protocols / / edited by Stefan Stamm, Chris Smith, and Reinhard Lührmann
| Alternative pre-mRNA splicing : theory and protocols / / edited by Stefan Stamm, Chris Smith, and Reinhard Lührmann |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Weinheim, Germany, : Wiley-Blackwell, c2012 |
| Descrizione fisica | 1 online resource (662 p.) |
| Disciplina | 572.88 |
| Altri autori (Persone) |
StammStefan
SmithChris LührmannReinhard |
| Soggetto topico | Messenger RNA |
| ISBN |
9786613432131
9783527647989 3527647988 9781283432139 1283432137 9783527636778 3527636773 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt. 1. Theory -- pt. 2. Basic methods -- pt. 3. Detection of splicing events -- pt. 4. Analysis of splicing in vitro -- pt. 5. Analysis of splicing in vivo -- pt. 6. Manipulation of splicing events -- pt. 7. Bioinformatic analysis of splicing. |
| Record Nr. | UNINA-9910821507803321 |
| Weinheim, Germany, : Wiley-Blackwell, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Antisense and ribozyme methodology / / Ian Gibson, editor
| Antisense and ribozyme methodology / / Ian Gibson, editor |
| Pubbl/distr/stampa | London, [England] : , : Chapman & Hall, , 1997 |
| Descrizione fisica | 1 online resource (96 p.) |
| Disciplina |
572.88
615.36 |
| Collana | Laboratory Companion Series |
| Soggetto topico |
Antisense nucleic acids
Catalytic RNA |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-84239-7
9786611842390 3-527-61253-X 3-527-61252-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Antisense and Ribozyme Methodology; Contents; CHAPTER 1. Antisense and Ribozyme Methodology; 1.1 The Potential; 1.2 Antisense Technology; 1.2.1 Problems; 1.2.2 Resistance to Nucleases; 1.2.3 Entry into Cells; 1.2.4 How Antisense Works; 1.2.5 Success; 1.3 Ribozymes; 1.3.1 What Are They?; 1.3.2 Problems; 1.3.3 Stable Ribozymes; 1.3.4 Designing Ribozymes; 1.4 Ribozymes or Antisense DNAs?; 1.5 The Choice Today!!; CHAPTER 2. Design and Synthesis of Antisense DNA Molecules; 2.1 Introduction; 2.2 Synthesis of Methylphosphonodiester-Phosphodiester Chimeric Oligodeoxynucleotides
2.2.1 Materials and Chemicals2.2.2 Solutions; 2.2.3 Maximizing Product Purity; 2.2.4 Deprotection of Chimeric Oligodeoxynucleotides; 2.2.5 Failed Sequences; 2.3 Primary Purification by Reversed-Phase, Solid-Phase Extraction on C18 SEP-PAK Cartridges; 2.3.1 Equipment; 2.3.2 Method; 2.3.3 Purification of the Oligodeoxynucleotide; 2.3.4 Further Purification; 2.4 Analysis and Purification by HPLC; 2.4.1 Analysis of Chimeric Oligodeoxynucleotides by HPLC; 2.4.2 Purification of Chimeric Oligodeoxynucleotides by HPLC; 2.4.3 Re-Use of Columns 2.5 Synthesis of Chimeric Oligodeoxynucleotides with Fluorescein Attached2.6 Summary; CHAPTER 3. The Design and Synthesis of Hammerhead Ribozymes; 3.1 Introduction; 3.2 The Design of Hammerhead Ribozymes; 3.3 Improving the Reactions; 3.3.1 Accessibility of the Target - Substrate Binding; 3.3.2 Finding the Target; 3.3.3 Theoretical Considerations; 3.3.4 Experimental Approaches; 3.3.5 Kinetic Studies; 3.4 Length of Arms; 3.4.1 Choosing Antisense Arms of Hammerhead Ribozymes; 3.4.2 Arms of Different Lengths; 3.5 Cleavage of the Target Motif; 3.6 Synthesis of Ribozymes 3.6.1 Chemical Synthesis of Short Hammerhead Ribozymes3.6.2 Enzymatic Transcription in Vitro; 3.7 Endogenous Expression of Ribozyme Genes; CHAPTER 4. Delivery of Ribozymes and Antisense DNA Molecules into Mammalian Cells; 4.1 Introduction; 4.2 Exogenous Application; 4.2.1 lntracytoplasmic Delivery of Antisense Oligodeoxynucleotides by Reversible Plasma Membrane Permeabilization with Streptolysin O; 4.3 Microinjection; 4.4 Other Methods Used in Nucleic Acid Transfection; 4.5 Electroporation; 4.5.1 Method; 4.5.2 Transfection: Optimization of Conditions 4.5.3 Mechanism of Uptake Following Electroporation4.5.4 Benefits and Drawbacks of Electrophoretic-Mediated Uptake; 4.6 Diethylaminoethyl-Dextran (DEAE- Dextran) and DNA Transfection; 4.6.1 Methods for Transfection of Adherent Cells; 4.6.2 Possible Alterations of the Above Protocol; 4.6.3 Transfection of Cells Growing in Suspension; 4.6.4 Transfection Optimization; 4.6.5 Distribution Mechanism of DEAE-Dextran Uptake and lntracellular; 4.6.6 Benefits and Drawbacks; 4.7 Calcium Phosphate Transfection; 4.7.1 Method; 4.7.2 Possible Alterations to Above Method; 4.7.3 Method Optimization 4.7.4 Calcium Phosphate-Mediated Uptake and lntracellular Distribution |
| Record Nr. | UNINA-9910144117903321 |
| London, [England] : , : Chapman & Hall, , 1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Antisense and ribozyme methodology / / Ian Gibson, editor
| Antisense and ribozyme methodology / / Ian Gibson, editor |
| Pubbl/distr/stampa | London, [England] : , : Chapman & Hall, , 1997 |
| Descrizione fisica | 1 online resource (96 p.) |
| Disciplina |
572.88
615.36 |
| Collana | Laboratory Companion Series |
| Soggetto topico |
Antisense nucleic acids
Catalytic RNA |
| ISBN |
1-281-84239-7
9786611842390 3-527-61253-X 3-527-61252-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Antisense and Ribozyme Methodology; Contents; CHAPTER 1. Antisense and Ribozyme Methodology; 1.1 The Potential; 1.2 Antisense Technology; 1.2.1 Problems; 1.2.2 Resistance to Nucleases; 1.2.3 Entry into Cells; 1.2.4 How Antisense Works; 1.2.5 Success; 1.3 Ribozymes; 1.3.1 What Are They?; 1.3.2 Problems; 1.3.3 Stable Ribozymes; 1.3.4 Designing Ribozymes; 1.4 Ribozymes or Antisense DNAs?; 1.5 The Choice Today!!; CHAPTER 2. Design and Synthesis of Antisense DNA Molecules; 2.1 Introduction; 2.2 Synthesis of Methylphosphonodiester-Phosphodiester Chimeric Oligodeoxynucleotides
2.2.1 Materials and Chemicals2.2.2 Solutions; 2.2.3 Maximizing Product Purity; 2.2.4 Deprotection of Chimeric Oligodeoxynucleotides; 2.2.5 Failed Sequences; 2.3 Primary Purification by Reversed-Phase, Solid-Phase Extraction on C18 SEP-PAK Cartridges; 2.3.1 Equipment; 2.3.2 Method; 2.3.3 Purification of the Oligodeoxynucleotide; 2.3.4 Further Purification; 2.4 Analysis and Purification by HPLC; 2.4.1 Analysis of Chimeric Oligodeoxynucleotides by HPLC; 2.4.2 Purification of Chimeric Oligodeoxynucleotides by HPLC; 2.4.3 Re-Use of Columns 2.5 Synthesis of Chimeric Oligodeoxynucleotides with Fluorescein Attached2.6 Summary; CHAPTER 3. The Design and Synthesis of Hammerhead Ribozymes; 3.1 Introduction; 3.2 The Design of Hammerhead Ribozymes; 3.3 Improving the Reactions; 3.3.1 Accessibility of the Target - Substrate Binding; 3.3.2 Finding the Target; 3.3.3 Theoretical Considerations; 3.3.4 Experimental Approaches; 3.3.5 Kinetic Studies; 3.4 Length of Arms; 3.4.1 Choosing Antisense Arms of Hammerhead Ribozymes; 3.4.2 Arms of Different Lengths; 3.5 Cleavage of the Target Motif; 3.6 Synthesis of Ribozymes 3.6.1 Chemical Synthesis of Short Hammerhead Ribozymes3.6.2 Enzymatic Transcription in Vitro; 3.7 Endogenous Expression of Ribozyme Genes; CHAPTER 4. Delivery of Ribozymes and Antisense DNA Molecules into Mammalian Cells; 4.1 Introduction; 4.2 Exogenous Application; 4.2.1 lntracytoplasmic Delivery of Antisense Oligodeoxynucleotides by Reversible Plasma Membrane Permeabilization with Streptolysin O; 4.3 Microinjection; 4.4 Other Methods Used in Nucleic Acid Transfection; 4.5 Electroporation; 4.5.1 Method; 4.5.2 Transfection: Optimization of Conditions 4.5.3 Mechanism of Uptake Following Electroporation4.5.4 Benefits and Drawbacks of Electrophoretic-Mediated Uptake; 4.6 Diethylaminoethyl-Dextran (DEAE- Dextran) and DNA Transfection; 4.6.1 Methods for Transfection of Adherent Cells; 4.6.2 Possible Alterations of the Above Protocol; 4.6.3 Transfection of Cells Growing in Suspension; 4.6.4 Transfection Optimization; 4.6.5 Distribution Mechanism of DEAE-Dextran Uptake and lntracellular; 4.6.6 Benefits and Drawbacks; 4.7 Calcium Phosphate Transfection; 4.7.1 Method; 4.7.2 Possible Alterations to Above Method; 4.7.3 Method Optimization 4.7.4 Calcium Phosphate-Mediated Uptake and lntracellular Distribution |
| Record Nr. | UNISA-996203927503316 |
| London, [England] : , : Chapman & Hall, , 1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Antisense and ribozyme methodology / / Ian Gibson, editor
| Antisense and ribozyme methodology / / Ian Gibson, editor |
| Pubbl/distr/stampa | London, [England] : , : Chapman & Hall, , 1997 |
| Descrizione fisica | 1 online resource (96 p.) |
| Disciplina |
572.88
615.36 |
| Collana | Laboratory Companion Series |
| Soggetto topico |
Antisense nucleic acids
Catalytic RNA |
| ISBN |
1-281-84239-7
9786611842390 3-527-61253-X 3-527-61252-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Antisense and Ribozyme Methodology; Contents; CHAPTER 1. Antisense and Ribozyme Methodology; 1.1 The Potential; 1.2 Antisense Technology; 1.2.1 Problems; 1.2.2 Resistance to Nucleases; 1.2.3 Entry into Cells; 1.2.4 How Antisense Works; 1.2.5 Success; 1.3 Ribozymes; 1.3.1 What Are They?; 1.3.2 Problems; 1.3.3 Stable Ribozymes; 1.3.4 Designing Ribozymes; 1.4 Ribozymes or Antisense DNAs?; 1.5 The Choice Today!!; CHAPTER 2. Design and Synthesis of Antisense DNA Molecules; 2.1 Introduction; 2.2 Synthesis of Methylphosphonodiester-Phosphodiester Chimeric Oligodeoxynucleotides
2.2.1 Materials and Chemicals2.2.2 Solutions; 2.2.3 Maximizing Product Purity; 2.2.4 Deprotection of Chimeric Oligodeoxynucleotides; 2.2.5 Failed Sequences; 2.3 Primary Purification by Reversed-Phase, Solid-Phase Extraction on C18 SEP-PAK Cartridges; 2.3.1 Equipment; 2.3.2 Method; 2.3.3 Purification of the Oligodeoxynucleotide; 2.3.4 Further Purification; 2.4 Analysis and Purification by HPLC; 2.4.1 Analysis of Chimeric Oligodeoxynucleotides by HPLC; 2.4.2 Purification of Chimeric Oligodeoxynucleotides by HPLC; 2.4.3 Re-Use of Columns 2.5 Synthesis of Chimeric Oligodeoxynucleotides with Fluorescein Attached2.6 Summary; CHAPTER 3. The Design and Synthesis of Hammerhead Ribozymes; 3.1 Introduction; 3.2 The Design of Hammerhead Ribozymes; 3.3 Improving the Reactions; 3.3.1 Accessibility of the Target - Substrate Binding; 3.3.2 Finding the Target; 3.3.3 Theoretical Considerations; 3.3.4 Experimental Approaches; 3.3.5 Kinetic Studies; 3.4 Length of Arms; 3.4.1 Choosing Antisense Arms of Hammerhead Ribozymes; 3.4.2 Arms of Different Lengths; 3.5 Cleavage of the Target Motif; 3.6 Synthesis of Ribozymes 3.6.1 Chemical Synthesis of Short Hammerhead Ribozymes3.6.2 Enzymatic Transcription in Vitro; 3.7 Endogenous Expression of Ribozyme Genes; CHAPTER 4. Delivery of Ribozymes and Antisense DNA Molecules into Mammalian Cells; 4.1 Introduction; 4.2 Exogenous Application; 4.2.1 lntracytoplasmic Delivery of Antisense Oligodeoxynucleotides by Reversible Plasma Membrane Permeabilization with Streptolysin O; 4.3 Microinjection; 4.4 Other Methods Used in Nucleic Acid Transfection; 4.5 Electroporation; 4.5.1 Method; 4.5.2 Transfection: Optimization of Conditions 4.5.3 Mechanism of Uptake Following Electroporation4.5.4 Benefits and Drawbacks of Electrophoretic-Mediated Uptake; 4.6 Diethylaminoethyl-Dextran (DEAE- Dextran) and DNA Transfection; 4.6.1 Methods for Transfection of Adherent Cells; 4.6.2 Possible Alterations of the Above Protocol; 4.6.3 Transfection of Cells Growing in Suspension; 4.6.4 Transfection Optimization; 4.6.5 Distribution Mechanism of DEAE-Dextran Uptake and lntracellular; 4.6.6 Benefits and Drawbacks; 4.7 Calcium Phosphate Transfection; 4.7.1 Method; 4.7.2 Possible Alterations to Above Method; 4.7.3 Method Optimization 4.7.4 Calcium Phosphate-Mediated Uptake and lntracellular Distribution |
| Record Nr. | UNINA-9910830630503321 |
| London, [England] : , : Chapman & Hall, , 1997 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Applications of RNA-Seq and Omics Strategies : From Microorganisms to Human Health / / edited by Fabio A. Marchi, Priscila D.R. Cirillo and Elvis C. Mateo
| Applications of RNA-Seq and Omics Strategies : From Microorganisms to Human Health / / edited by Fabio A. Marchi, Priscila D.R. Cirillo and Elvis C. Mateo |
| Autore | Elvis C. Mateo |
| Pubbl/distr/stampa | IntechOpen, 2017 |
| Descrizione fisica | 1 online resource (vii, 328 pages) : illustrations(some color) |
| Disciplina | 572.88 |
| Soggetto topico |
Genetics
RNA |
| Soggetto non controllato |
Life Sciences
Microbiology Genetics and Molecular Biology Biochemistry Microbial Genetics |
| ISBN |
953-51-4652-1
953-51-3504-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Altri titoli varianti | Applications of RNA-Seq and omics strategies |
| Record Nr. | UNINA-9910251415403321 |
Elvis C. Mateo
|
||
| IntechOpen, 2017 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Applied RNA Bioscience / / edited by Seiji Masuda, Shingo Izawa
| Applied RNA Bioscience / / edited by Seiji Masuda, Shingo Izawa |
| Edizione | [1st ed. 2018.] |
| Pubbl/distr/stampa | Singapore : , : Springer Singapore : , : Imprint : Springer, , 2018 |
| Descrizione fisica | 1 online resource (286 pages) |
| Disciplina | 572.88 |
| Soggetto topico |
Nucleic acids
Human genetics Biotechnology Cytology Molecular biology Nucleic Acid Chemistry Human Genetics Cell Biology Molecular Medicine |
| ISBN | 981-10-8372-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910298439203321 |
| Singapore : , : Springer Singapore : , : Imprint : Springer, , 2018 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biological relevance & therapeutic applications of DNA- & RNA-quadruplexes / / editor David Monchaud
| Biological relevance & therapeutic applications of DNA- & RNA-quadruplexes / / editor David Monchaud |
| Pubbl/distr/stampa | London, England : , : Future Science Ltd, , 2015 |
| Descrizione fisica | 1 online resource (248 pages) : illustrations |
| Disciplina | 572.88 |
| Collana | Future Science |
| Soggetto topico |
RNA - Research
DNA - Analysis |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-910419-69-9
1-910419-68-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910467825803321 |
| London, England : , : Future Science Ltd, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biological relevance & therapeutic applications of DNA- & RNA-quadruplexes / / editor David Monchaud
| Biological relevance & therapeutic applications of DNA- & RNA-quadruplexes / / editor David Monchaud |
| Pubbl/distr/stampa | London, England : , : Future Science Ltd, , 2015 |
| Descrizione fisica | 1 online resource (248 pages) : illustrations |
| Disciplina | 572.88 |
| Collana | Future Science |
| Soggetto topico |
RNA - Research
DNA - Analysis |
| ISBN |
1-910419-69-9
1-910419-68-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910794873403321 |
| London, England : , : Future Science Ltd, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biological relevance & therapeutic applications of DNA- & RNA-quadruplexes / / editor David Monchaud
| Biological relevance & therapeutic applications of DNA- & RNA-quadruplexes / / editor David Monchaud |
| Pubbl/distr/stampa | London, England : , : Future Science Ltd, , 2015 |
| Descrizione fisica | 1 online resource (248 pages) : illustrations |
| Disciplina | 572.88 |
| Collana | Future Science |
| Soggetto topico |
RNA - Research
DNA - Analysis |
| ISBN |
1-910419-69-9
1-910419-68-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910820796803321 |
| London, England : , : Future Science Ltd, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
