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Analysing gene expression [[electronic resource] ] : a handbook of methods: possibilities and pitfalls / / edited by Stefan Lorkowski and Paul Cullen
| Analysing gene expression [[electronic resource] ] : a handbook of methods: possibilities and pitfalls / / edited by Stefan Lorkowski and Paul Cullen |
| Pubbl/distr/stampa | Weinheim ; ; New York, : Wiley-VCH, c2003 |
| Descrizione fisica | 1 online resource (984 p.) |
| Disciplina |
572.8/65
572.865 |
| Altri autori (Persone) |
LorkowskiStefan
CullenPaul, Dr. |
| Soggetto topico | Gene expression |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-52042-6
9786610520428 3-527-60533-9 3-527-60149-X |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Analysing Gene Expression A Handbook of Methods Possibilities and Pitfalls; Foreword; Preface; Contents; List of Contributors; Volume 1; 1 Basic concepts of gene expression; 1.1 Introduction; 1.2 Basics of transcription and translation in the cell; 1.2.1 Introduction; 1.2.2 Transcription; 1.2.3 Translation; 1.2.4 Summary; 1.3 Regulation of transcription; 1.3.1 Introduction; 1.3.2 mRNA expression profiles - the transcriptome; 1.3.3 Protein expression profiles - the proteome; 1.3.4 Interaction between genes and proteins - the interactome; 1.3.5 The transcription machinery and core promoters
1.3.6 Regulatory promoters1.3.7 Enhancers; 1.3.8 Locus control regions; 1.3.9 Matrix attachment regions; 1.3.10 Insulators; 1.3.11 RIDGEs - Regions of increased gene expression; 1.3.12 Enhanceosomes; 1.3.13 Chromatin; 1.3.14 Silencer elements; 1.3.15 Transcription factors, repressors and co-repressors; 1.3.16 Epigenetics; 1.3.17 Summary and conclusions; 1.4 Post-transcriptional regulation; 1.4.1 Introduction; 1.4.2 Regulation of RNA stability and degradation; 1.4.3 Regulation of transcription elongation; 1.4.3.1 Introduction; 1.4.3.2 Regulation of transcription elongation in prokaryotes 1.4.3.3 Regulation of transcription elongation in eukaryotes1.4.3.4 Conclusions; 1.4.4 Differential/alternative pre-mRNA splicing; 1.4.5 Trans-RNA splicing; 1.4.6 Regulation of mRNA transport; 1.4.7 Directed intracellular mRNA localisation; 1.4.8 Regulation of mRNA polyadenylation; 1.4.9 Antisense RNA; 1.4.10 RNA editing; 1.4.11 Summary and conclusions; 1.5 Post-translational modification of proteins; 1.5.1 Introduction; 1.5.2 Proteolytic cleavage of proteins; 1.5.3 Acylation; 1.5.4 Prenylation; 1.5.5 Methylation; 1.5.6 Sulphation; 1.5.7 Phosphorylation; 1.5.8 Ubiquitination 1.5.9 Glycosylation1.5.10 Conclusions; 1.6 Correlation of mRNA and protein expression; 1.6.1 Introduction; 1.6.2 Levels of mRNA and protein expression: correlations and discrepancies; 1.6.3 Conclusions; 1.7 Housekeeping genes, internal and external standards; 1.7.1 What are housekeeping genes?; 1.7.2 Survey of the most important housekeeping genes; 1.7.2.1 Glyceraldehyde-3-phosphate dehydrogenase; 1.7.2.2 β-Actin; 1.7.3 Other commonly used housekeeping genes; 1.7.3.1 Ribosomal RNA (rRNA); 1.7.4 New identified 'maintenance genes'; 1.7.5 Methods of quantification 1.7.5.1 Normalisation (via internal standards)1.7.5.2 Standard curves (via external standards); 1.7.5.3 Alternative methods (via internal standards); 1.7.6 Summary; 1.8 Classification of differential gene expression technologies; 1.8.1 Introduction; 1.8.2 From single genes to transcriptomes; 1.8.3 Methods of classification; 1.8.4 Conclusions; 1.9 Summary; 1.10 References; 2 Sample preparation and supplementary tools; 2.1 Introduction; 2.2 Preparation of cells and tissues; 2.2.1 Immunopurification of cells; 2.2.2 Differential-velocity centrifugation/countercurrent elutriation 2.2.3 Surface affinity chromatography |
| Record Nr. | UNINA-9910146242103321 |
| Weinheim ; ; New York, : Wiley-VCH, c2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Analysing gene expression [[electronic resource] ] : a handbook of methods: possibilities and pitfalls / / edited by Stefan Lorkowski and Paul Cullen
| Analysing gene expression [[electronic resource] ] : a handbook of methods: possibilities and pitfalls / / edited by Stefan Lorkowski and Paul Cullen |
| Pubbl/distr/stampa | Weinheim ; ; New York, : Wiley-VCH, c2003 |
| Descrizione fisica | 1 online resource (984 p.) |
| Disciplina |
572.8/65
572.865 |
| Altri autori (Persone) |
LorkowskiStefan
CullenPaul, Dr. |
| Soggetto topico | Gene expression |
| ISBN |
1-280-52042-6
9786610520428 3-527-60533-9 3-527-60149-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Analysing Gene Expression A Handbook of Methods Possibilities and Pitfalls; Foreword; Preface; Contents; List of Contributors; Volume 1; 1 Basic concepts of gene expression; 1.1 Introduction; 1.2 Basics of transcription and translation in the cell; 1.2.1 Introduction; 1.2.2 Transcription; 1.2.3 Translation; 1.2.4 Summary; 1.3 Regulation of transcription; 1.3.1 Introduction; 1.3.2 mRNA expression profiles - the transcriptome; 1.3.3 Protein expression profiles - the proteome; 1.3.4 Interaction between genes and proteins - the interactome; 1.3.5 The transcription machinery and core promoters
1.3.6 Regulatory promoters1.3.7 Enhancers; 1.3.8 Locus control regions; 1.3.9 Matrix attachment regions; 1.3.10 Insulators; 1.3.11 RIDGEs - Regions of increased gene expression; 1.3.12 Enhanceosomes; 1.3.13 Chromatin; 1.3.14 Silencer elements; 1.3.15 Transcription factors, repressors and co-repressors; 1.3.16 Epigenetics; 1.3.17 Summary and conclusions; 1.4 Post-transcriptional regulation; 1.4.1 Introduction; 1.4.2 Regulation of RNA stability and degradation; 1.4.3 Regulation of transcription elongation; 1.4.3.1 Introduction; 1.4.3.2 Regulation of transcription elongation in prokaryotes 1.4.3.3 Regulation of transcription elongation in eukaryotes1.4.3.4 Conclusions; 1.4.4 Differential/alternative pre-mRNA splicing; 1.4.5 Trans-RNA splicing; 1.4.6 Regulation of mRNA transport; 1.4.7 Directed intracellular mRNA localisation; 1.4.8 Regulation of mRNA polyadenylation; 1.4.9 Antisense RNA; 1.4.10 RNA editing; 1.4.11 Summary and conclusions; 1.5 Post-translational modification of proteins; 1.5.1 Introduction; 1.5.2 Proteolytic cleavage of proteins; 1.5.3 Acylation; 1.5.4 Prenylation; 1.5.5 Methylation; 1.5.6 Sulphation; 1.5.7 Phosphorylation; 1.5.8 Ubiquitination 1.5.9 Glycosylation1.5.10 Conclusions; 1.6 Correlation of mRNA and protein expression; 1.6.1 Introduction; 1.6.2 Levels of mRNA and protein expression: correlations and discrepancies; 1.6.3 Conclusions; 1.7 Housekeeping genes, internal and external standards; 1.7.1 What are housekeeping genes?; 1.7.2 Survey of the most important housekeeping genes; 1.7.2.1 Glyceraldehyde-3-phosphate dehydrogenase; 1.7.2.2 β-Actin; 1.7.3 Other commonly used housekeeping genes; 1.7.3.1 Ribosomal RNA (rRNA); 1.7.4 New identified 'maintenance genes'; 1.7.5 Methods of quantification 1.7.5.1 Normalisation (via internal standards)1.7.5.2 Standard curves (via external standards); 1.7.5.3 Alternative methods (via internal standards); 1.7.6 Summary; 1.8 Classification of differential gene expression technologies; 1.8.1 Introduction; 1.8.2 From single genes to transcriptomes; 1.8.3 Methods of classification; 1.8.4 Conclusions; 1.9 Summary; 1.10 References; 2 Sample preparation and supplementary tools; 2.1 Introduction; 2.2 Preparation of cells and tissues; 2.2.1 Immunopurification of cells; 2.2.2 Differential-velocity centrifugation/countercurrent elutriation 2.2.3 Surface affinity chromatography |
| Record Nr. | UNINA-9910830158703321 |
| Weinheim ; ; New York, : Wiley-VCH, c2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Analysis and Design of Delayed Genetic Regulatory Networks / / by Xian Zhang, Yantao Wang, Ligang Wu
| Analysis and Design of Delayed Genetic Regulatory Networks / / by Xian Zhang, Yantao Wang, Ligang Wu |
| Autore | Zhang Xian |
| Edizione | [1st ed. 2019.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2019 |
| Descrizione fisica | 1 online resource (275 pages) |
| Disciplina | 572.865 |
| Collana | Studies in Systems, Decision and Control |
| Soggetto topico |
Vibration
Dynamical systems Dynamics Computational intelligence Vibration, Dynamical Systems, Control Computational Intelligence |
| ISBN | 3-030-17098-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Stability Analysis for GRNs with Mixed Delays -- Stability Analysis of Delayed GRNs -- Stability Analysis for Delayed Switching GRNs -- Stability Analysis for Delayed Stochastic GRNs -- Stability Analysis for Delayed Reaction-Diffusion GRNs -- State Estimation for Delayed GRNs -- Guaranteed Cost Control for Delayed GRNs -- State Estimation for Delayed Reaction-Diffusion GRNs -- H State Estimation for Delayed Stochastic GRNs -- H State Estimation for Delayed Discrete-Time GRNs. |
| Record Nr. | UNINA-9910337614403321 |
Zhang Xian
|
||
| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2019 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Analyzing microarray gene expression data [[electronic resource] /] / Geoffrey J. McLachlan, Kim-Anh Do, Christopher Ambroise
| Analyzing microarray gene expression data [[electronic resource] /] / Geoffrey J. McLachlan, Kim-Anh Do, Christopher Ambroise |
| Autore | McLachlan Geoffrey J. <1946-> |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2004 |
| Descrizione fisica | 1 online resource (366 p.) |
| Disciplina |
572.8636
572.865 |
| Altri autori (Persone) |
DoKim-Anh <1960->
AmbroiseChristophe <1969-> |
| Collana | Wiley series in probability and statistics |
| Soggetto topico |
DNA microarrays - Statistical methods
Gene expression - Statistical methods |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-25332-0
9786610253326 0-470-35030-X 0-471-72612-5 0-471-72842-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Analyzing Microarray Gene Expression Data; Contents; Preface; 1 Microarrays in Gene Expression Studies; 1.1 Introduction; 1.2 Background Biology; 1.2.1 Genome, Genotype, and Gene Expression; 1.2.2 Of Wild-Types and Other Alleles; 1.2.3 Aspects of Underlying Biology and Physiochemistry; 1.3 Polymerase Chain Reaction; 1.4 cDNA; 1.4.1 Expressed Sequence Tag; 1.5 Microarray Technology and Application; 1.5.1 History of Microarray Development; 1.5.2 Tools of Microarray Technology; 1.5.3 Limitations of Microarray Technology; 1.5.4 Oligonucleotides versus cDNA Arrays
1.5.5 SAGE: Another Method for Detecting and Measuring Gene Expression Levels1.5.6 Emerging Technologies; 1.6 Sampling of Relevant Research Entities and Public Resources; 2 Cleaning and Normalization; 2.1 Introduction; 2.2 Cleaning Procedures; 2.2.1 Image Processing to Extract Information; 2.2.2 Missing Value Estimation; 2.2.3 Sources of Nonlinearity; 2.3 Normalization and Plotting Procedures for Oligonucleotide Arrays; 2.3.1 Global Approaches for Oligonucleotide Array Data; 2.3.2 Spiked Standard Approaches; 2.3.3 Geometric Mean and Linear Regression Normalization for Multiple Arrays 2.3.4 Nonlinear Normalization for Multiple Arrays Using Smooth Curves2.4 Normalization Methods for cDNA Microarray Data; 2.4.1 Single-Array Normalization; 2.4.2 Multiple Slides Normalization; 2.4.3 ANOVA and Related Methods for Normalization; 2.4.4 Mixed-Model Method for Normalization; 2.4.5 SNOMAD; 2.5 Transformations and Replication; 2.5.1 Importance of Replication; 2.5.2 Transformations; 2.6 Analysis of the Alon Data Set; 2.7 Comparison of Normalization Strategies and Discussion; 3 Some Cluster Analysis Methods; 3.1 Introduction; 3.2 Reduction in the Dimension of the Feature Space 3.3 Cluster Analysis3.4 Some Hierarchical Agglomerative Techniques; 3.5 k-Means Clustering; 3.6 Cluster Analysis with No A Priori Metric; 3.7 Clustering via Finite Mixture Models; 3.7.1 Definition; 3.7.2 Advantages of Model-Based Clustering; 3.8 Fitting Mixture Models Via the EM Algorithm; 3.8.1 E-Step; 3.8.2 M-Step; 3.8.3 Choice of Starting Values for the EM Algorithm; 3.9 Clustering Via Normal Mixtures; 3.9.1 Heteroscedastic Components; 3.9.2 Homoscedastic Components; 3.9.3 Spherical Components; 3.9.4 Choice of Root; 3.9.5 Available Software; 3.10 Mixtures of t Distributions 3.11 Mixtures of Factor Analyzers3.12 Choice of Clustering Solution; 3.13 Classification ML Approach; 3.14 Mixture Models for Clinical and Microarray Data; 3.14.1 Unconditional Approach; 3.14.2 Conditional Approach; 3.15 Choice of the Number of Components in a Mixture Model; 3.15.1 Order of a Mixture Model; 3.15.2 Approaches for Assessing Mixture Order; 3.15.3 Bayesian Information Criterion; 3.15.4 Integrated Classification Likelihood Criterion; 3.16 Resampling Approach; 3.17 Other Resampling Approaches for Number of Clusters; 3.17.1 The Gap Statistic 3.17.2 The Clest Method for the Number of Clusters |
| Record Nr. | UNINA-9910146082203321 |
|
McLachlan Geoffrey J. <1946->
|
||
| Hoboken, N.J., : Wiley-Interscience, c2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Analyzing microarray gene expression data [[electronic resource] /] / Geoffrey J. McLachlan, Kim-Anh Do, Christopher Ambroise
| Analyzing microarray gene expression data [[electronic resource] /] / Geoffrey J. McLachlan, Kim-Anh Do, Christopher Ambroise |
| Autore | McLachlan Geoffrey J. <1946-> |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Interscience, c2004 |
| Descrizione fisica | 1 online resource (366 p.) |
| Disciplina |
572.8636
572.865 |
| Altri autori (Persone) |
DoKim-Anh <1960->
AmbroiseChristophe <1969-> |
| Collana | Wiley series in probability and statistics |
| Soggetto topico |
DNA microarrays - Statistical methods
Gene expression - Statistical methods |
| ISBN |
1-280-25332-0
9786610253326 0-470-35030-X 0-471-72612-5 0-471-72842-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Analyzing Microarray Gene Expression Data; Contents; Preface; 1 Microarrays in Gene Expression Studies; 1.1 Introduction; 1.2 Background Biology; 1.2.1 Genome, Genotype, and Gene Expression; 1.2.2 Of Wild-Types and Other Alleles; 1.2.3 Aspects of Underlying Biology and Physiochemistry; 1.3 Polymerase Chain Reaction; 1.4 cDNA; 1.4.1 Expressed Sequence Tag; 1.5 Microarray Technology and Application; 1.5.1 History of Microarray Development; 1.5.2 Tools of Microarray Technology; 1.5.3 Limitations of Microarray Technology; 1.5.4 Oligonucleotides versus cDNA Arrays
1.5.5 SAGE: Another Method for Detecting and Measuring Gene Expression Levels1.5.6 Emerging Technologies; 1.6 Sampling of Relevant Research Entities and Public Resources; 2 Cleaning and Normalization; 2.1 Introduction; 2.2 Cleaning Procedures; 2.2.1 Image Processing to Extract Information; 2.2.2 Missing Value Estimation; 2.2.3 Sources of Nonlinearity; 2.3 Normalization and Plotting Procedures for Oligonucleotide Arrays; 2.3.1 Global Approaches for Oligonucleotide Array Data; 2.3.2 Spiked Standard Approaches; 2.3.3 Geometric Mean and Linear Regression Normalization for Multiple Arrays 2.3.4 Nonlinear Normalization for Multiple Arrays Using Smooth Curves2.4 Normalization Methods for cDNA Microarray Data; 2.4.1 Single-Array Normalization; 2.4.2 Multiple Slides Normalization; 2.4.3 ANOVA and Related Methods for Normalization; 2.4.4 Mixed-Model Method for Normalization; 2.4.5 SNOMAD; 2.5 Transformations and Replication; 2.5.1 Importance of Replication; 2.5.2 Transformations; 2.6 Analysis of the Alon Data Set; 2.7 Comparison of Normalization Strategies and Discussion; 3 Some Cluster Analysis Methods; 3.1 Introduction; 3.2 Reduction in the Dimension of the Feature Space 3.3 Cluster Analysis3.4 Some Hierarchical Agglomerative Techniques; 3.5 k-Means Clustering; 3.6 Cluster Analysis with No A Priori Metric; 3.7 Clustering via Finite Mixture Models; 3.7.1 Definition; 3.7.2 Advantages of Model-Based Clustering; 3.8 Fitting Mixture Models Via the EM Algorithm; 3.8.1 E-Step; 3.8.2 M-Step; 3.8.3 Choice of Starting Values for the EM Algorithm; 3.9 Clustering Via Normal Mixtures; 3.9.1 Heteroscedastic Components; 3.9.2 Homoscedastic Components; 3.9.3 Spherical Components; 3.9.4 Choice of Root; 3.9.5 Available Software; 3.10 Mixtures of t Distributions 3.11 Mixtures of Factor Analyzers3.12 Choice of Clustering Solution; 3.13 Classification ML Approach; 3.14 Mixture Models for Clinical and Microarray Data; 3.14.1 Unconditional Approach; 3.14.2 Conditional Approach; 3.15 Choice of the Number of Components in a Mixture Model; 3.15.1 Order of a Mixture Model; 3.15.2 Approaches for Assessing Mixture Order; 3.15.3 Bayesian Information Criterion; 3.15.4 Integrated Classification Likelihood Criterion; 3.16 Resampling Approach; 3.17 Other Resampling Approaches for Number of Clusters; 3.17.1 The Gap Statistic 3.17.2 The Clest Method for the Number of Clusters |
| Record Nr. | UNINA-9910830637803321 |
|
McLachlan Geoffrey J. <1946->
|
||
| Hoboken, N.J., : Wiley-Interscience, c2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Applied RNAi : from fundamental research to therapeutic applications / / Edited by Patrick Arbuthnot and Marc S. Weinberg
| Applied RNAi : from fundamental research to therapeutic applications / / Edited by Patrick Arbuthnot and Marc S. Weinberg |
| Pubbl/distr/stampa | Norfolk, England : , : Caister Academic Press, , [2014] |
| Descrizione fisica | 1 online resource (266 p.) |
| Disciplina | 572.865 |
| Soggetto topico |
Gene silencing
RNA |
| Soggetto genere / forma | Electronic books. |
| ISBN | 1-908230-67-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Contents; Contributors; Preface; Current books of interest; 1: Overview of Biogenesis and Applications of MicroRNA; Introduction; Mammalian microRNA biogenesis; Regulation during normal and potentially pathogenic microRNA biogenesis; Harnessing the RNAi pathway for gene silencing; Augmenting and antagonizing microRNA function; MicroRNA targets as gene regulators and sensors; MicroRNAs as prognostic and diagnostic markers; Conclusion; 2: Non-canonical MicroRNA Biogenesis and Function; Canonical microRNA biogenesis and function; Other small RNA biogenesis pathways
Non-canonical microRNA biogenesisOther RNA modifications; Non-canonical microRNA function; Concluding remarks; 3: Non-coding RNAs and the Epigenetic Control of Gene Expression; Long non-coding RNAs epigenetic regulators in human cells; Disruption of long antisense ncRNA networks; The therapeutic potential of targeting antisense ncRNAs; Issues with using small antisense ncRNAs therapeutically; 4: From Mice to Men: Towards the Clinical Translation of MicroRNA Technologies for Somatic Cell Reprogramming; Introduction; General evidence for a role of miRNAs in pluripotency and differentiation Strategies for the identification of pluripotency-associated miRNAsDelivery or expression of miRNAs as novel tools to improve reprogramming; MiRNA-based systems to monitor or control pluripotency and differentiation; Outlook: the road towards clinical translation of human iPSC technologies; 5: Systems Biology Tools to Understand the Role of Host MicroRNAs in Infection: a Closer Look at HIV; Background information; Systems biology tools to investigate differential miRNA expression in HIV infection; A high-content screening approach to evaluate the role of host miRNAs in HIV infection Conclusions and future prospects6: Synthetic MicroRNA Blocking Agents; Introduction; Anti-miR chemistries; Mechanisms of action and delivery of anti-miRs into cells; Validation of anti-miR activity in cells and assays; Therapeutic anti-miRs and in vivo delivery; MicroRNA sponges for suppression of microRNA activity; Target protectors for inhibition of microRNA-induced mRNA degradation; MicroRNA mimics; Prospects; 7: Exploiting MicroRNAs to Regulate Transgene Expression; Introduction; Using microRNAs avoid immune response to transgene product after gene transfer Using microRNA to improve cell specificity and escape side-effect of gene transferNovel type of expression system using microRNAs; Conclusion; 8: Use of Artificial MicroRNAs for Gene Silencing; Introduction; Evolution of artificial miRNA as therapeutic agents; Application of amiRNA; Challenges using amiRNAs as therapeutic agents; amiRNA progress to clinic; Conclusions; 9: Harnessing RNAi for the Treatment of Viral Infections; Introduction; RNAi to treat viral hepatitis; RNAi for respiratory viruses; RNAi for haemorrhagic fever viruses; Other viruses targeted by RNAi RNAi for the treatment of non-viral infections |
| Record Nr. | UNINA-9910464383103321 |
| Norfolk, England : , : Caister Academic Press, , [2014] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Applied RNAi : from fundamental research to therapeutic applications / / Edited by Patrick Arbuthnot and Marc S. Weinberg
| Applied RNAi : from fundamental research to therapeutic applications / / Edited by Patrick Arbuthnot and Marc S. Weinberg |
| Pubbl/distr/stampa | Norfolk, England : , : Caister Academic Press, , [2014] |
| Descrizione fisica | 1 online resource (266 p.) |
| Disciplina | 572.865 |
| Soggetto topico |
Gene silencing
RNA |
| ISBN | 1-908230-67-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Contents; Contributors; Preface; Current books of interest; 1: Overview of Biogenesis and Applications of MicroRNA; Introduction; Mammalian microRNA biogenesis; Regulation during normal and potentially pathogenic microRNA biogenesis; Harnessing the RNAi pathway for gene silencing; Augmenting and antagonizing microRNA function; MicroRNA targets as gene regulators and sensors; MicroRNAs as prognostic and diagnostic markers; Conclusion; 2: Non-canonical MicroRNA Biogenesis and Function; Canonical microRNA biogenesis and function; Other small RNA biogenesis pathways
Non-canonical microRNA biogenesisOther RNA modifications; Non-canonical microRNA function; Concluding remarks; 3: Non-coding RNAs and the Epigenetic Control of Gene Expression; Long non-coding RNAs epigenetic regulators in human cells; Disruption of long antisense ncRNA networks; The therapeutic potential of targeting antisense ncRNAs; Issues with using small antisense ncRNAs therapeutically; 4: From Mice to Men: Towards the Clinical Translation of MicroRNA Technologies for Somatic Cell Reprogramming; Introduction; General evidence for a role of miRNAs in pluripotency and differentiation Strategies for the identification of pluripotency-associated miRNAsDelivery or expression of miRNAs as novel tools to improve reprogramming; MiRNA-based systems to monitor or control pluripotency and differentiation; Outlook: the road towards clinical translation of human iPSC technologies; 5: Systems Biology Tools to Understand the Role of Host MicroRNAs in Infection: a Closer Look at HIV; Background information; Systems biology tools to investigate differential miRNA expression in HIV infection; A high-content screening approach to evaluate the role of host miRNAs in HIV infection Conclusions and future prospects6: Synthetic MicroRNA Blocking Agents; Introduction; Anti-miR chemistries; Mechanisms of action and delivery of anti-miRs into cells; Validation of anti-miR activity in cells and assays; Therapeutic anti-miRs and in vivo delivery; MicroRNA sponges for suppression of microRNA activity; Target protectors for inhibition of microRNA-induced mRNA degradation; MicroRNA mimics; Prospects; 7: Exploiting MicroRNAs to Regulate Transgene Expression; Introduction; Using microRNAs avoid immune response to transgene product after gene transfer Using microRNA to improve cell specificity and escape side-effect of gene transferNovel type of expression system using microRNAs; Conclusion; 8: Use of Artificial MicroRNAs for Gene Silencing; Introduction; Evolution of artificial miRNA as therapeutic agents; Application of amiRNA; Challenges using amiRNAs as therapeutic agents; amiRNA progress to clinic; Conclusions; 9: Harnessing RNAi for the Treatment of Viral Infections; Introduction; RNAi to treat viral hepatitis; RNAi for respiratory viruses; RNAi for haemorrhagic fever viruses; Other viruses targeted by RNAi RNAi for the treatment of non-viral infections |
| Record Nr. | UNINA-9910786928003321 |
| Norfolk, England : , : Caister Academic Press, , [2014] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Applied RNAi : from fundamental research to therapeutic applications / / Edited by Patrick Arbuthnot and Marc S. Weinberg
| Applied RNAi : from fundamental research to therapeutic applications / / Edited by Patrick Arbuthnot and Marc S. Weinberg |
| Pubbl/distr/stampa | Norfolk, England : , : Caister Academic Press, , [2014] |
| Descrizione fisica | 1 online resource (266 p.) |
| Disciplina | 572.865 |
| Soggetto topico |
Gene silencing
RNA |
| ISBN | 1-908230-67-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Contents; Contributors; Preface; Current books of interest; 1: Overview of Biogenesis and Applications of MicroRNA; Introduction; Mammalian microRNA biogenesis; Regulation during normal and potentially pathogenic microRNA biogenesis; Harnessing the RNAi pathway for gene silencing; Augmenting and antagonizing microRNA function; MicroRNA targets as gene regulators and sensors; MicroRNAs as prognostic and diagnostic markers; Conclusion; 2: Non-canonical MicroRNA Biogenesis and Function; Canonical microRNA biogenesis and function; Other small RNA biogenesis pathways
Non-canonical microRNA biogenesisOther RNA modifications; Non-canonical microRNA function; Concluding remarks; 3: Non-coding RNAs and the Epigenetic Control of Gene Expression; Long non-coding RNAs epigenetic regulators in human cells; Disruption of long antisense ncRNA networks; The therapeutic potential of targeting antisense ncRNAs; Issues with using small antisense ncRNAs therapeutically; 4: From Mice to Men: Towards the Clinical Translation of MicroRNA Technologies for Somatic Cell Reprogramming; Introduction; General evidence for a role of miRNAs in pluripotency and differentiation Strategies for the identification of pluripotency-associated miRNAsDelivery or expression of miRNAs as novel tools to improve reprogramming; MiRNA-based systems to monitor or control pluripotency and differentiation; Outlook: the road towards clinical translation of human iPSC technologies; 5: Systems Biology Tools to Understand the Role of Host MicroRNAs in Infection: a Closer Look at HIV; Background information; Systems biology tools to investigate differential miRNA expression in HIV infection; A high-content screening approach to evaluate the role of host miRNAs in HIV infection Conclusions and future prospects6: Synthetic MicroRNA Blocking Agents; Introduction; Anti-miR chemistries; Mechanisms of action and delivery of anti-miRs into cells; Validation of anti-miR activity in cells and assays; Therapeutic anti-miRs and in vivo delivery; MicroRNA sponges for suppression of microRNA activity; Target protectors for inhibition of microRNA-induced mRNA degradation; MicroRNA mimics; Prospects; 7: Exploiting MicroRNAs to Regulate Transgene Expression; Introduction; Using microRNAs avoid immune response to transgene product after gene transfer Using microRNA to improve cell specificity and escape side-effect of gene transferNovel type of expression system using microRNAs; Conclusion; 8: Use of Artificial MicroRNAs for Gene Silencing; Introduction; Evolution of artificial miRNA as therapeutic agents; Application of amiRNA; Challenges using amiRNAs as therapeutic agents; amiRNA progress to clinic; Conclusions; 9: Harnessing RNAi for the Treatment of Viral Infections; Introduction; RNAi to treat viral hepatitis; RNAi for respiratory viruses; RNAi for haemorrhagic fever viruses; Other viruses targeted by RNAi RNAi for the treatment of non-viral infections |
| Record Nr. | UNINA-9910817216603321 |
| Norfolk, England : , : Caister Academic Press, , [2014] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Chemical Epigenetics / / edited by Antonello Mai
| Chemical Epigenetics / / edited by Antonello Mai |
| Edizione | [1st ed. 2020.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2020 |
| Descrizione fisica | 1 online resource (IX, 567 p. 80 illus., 50 illus. in color.) |
| Disciplina |
572.86
572.865 |
| Collana | Topics in Medicinal Chemistry |
| Soggetto topico |
Medicinal chemistry
Genetics Enzymology Chemical engineering Medicinal Chemistry Genetics and Genomics Industrial Chemistry/Chemical Engineering |
| ISBN | 3-030-42982-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Targeting the Zinc-Dependent Histone Deacetylases (HDACs) for Drug Discovery -- Hydroxamic Acid-Containing Peptides in the Study of Histone Deacetylases -- Sirtuin Inhibitors and Activators -- Histone Acetyltransferase Enzymes: From Biological Implications to Most Relevant Inhibitors -- Lysine Methyltransferases and Their Inhibitors -- PRMT Inhibitors -- Lysine-Specific Histone Demethylases 1/2 (LSD1/2) and Their Inhibitors -- Inhibitors of JmjC-Containing Histone Demethylases -- Chemical Compounds Targeting DNA Methylation and Hydroxymethylation -- Applied Biophysics for Bromodomain Drug Discovery -- Methyl-Readers and Inhibitors -- Altered Long Non-coding RNA Expression in Cancer: Potential Biomarkers and Therapeutic Targets? - Acetylation and Methylation in Asthma, COPD, and Lung Cancer -- Structure-Based Design ofEpigenetic Inhibitors -- Experimental Methodologies for Detection and Mapping of Epigenetic DNA Marks -- Advanced Assays in Epigenetics. |
| Record Nr. | UNINA-9910410051303321 |
| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2020 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Chromatin and gene regulation [[electronic resource] ] : mechanisms in epigenetics / / Bryan M. Turner
| Chromatin and gene regulation [[electronic resource] ] : mechanisms in epigenetics / / Bryan M. Turner |
| Autore | Turner Bryan M |
| Pubbl/distr/stampa | Oxford ; ; Malden, MA, : Blackwell Science, 2001 |
| Descrizione fisica | 1 online resource (298 p.) |
| Disciplina | 572.865 |
| Soggetto topico |
Chromatin
Genetic regulation |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-32244-X
9786611322441 0-470-75062-6 0-470-75167-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Chromatin and Gene Regulation; Contents; Preface; Prologue; Chapter 1 Controlling Transcription: Shared Aims and Common Mechanisms; Introduction; Some general principles; Transcription in prokaryotes; Genetic switches in bacteria; Chapter 2 Transcription in Eukaryotes: The Problems of Complexity; Introduction; The emergence of eukaryotes; The transcription machinery in eukaryotes; General transcription factors, TAFs and the PolII pre-initiation complex; Transcription by PolI and PolIII; The elongation stage; Experimental considerations; Large genome problems: why are things so complicated?
Chapter 3 The Nucleosome: Chromatin's Structural UnitIntroduction; Exploring how DNA is packaged in the nucleus; The structure of the nucleosome; Chapter 4 Histone Tails: Modifications and Epigenetic Information; Introduction; The histone tails; Histone modifications; Histone variants; Chapter 5 Higher-Order Chromatin Structures and Nuclear Organization; Introduction; The 30 nm fibre; DNA loops; The nuclear matrix and chromosome scaffolds; Scaffold/matrix associated regions (SARs and MARs); Chromosome bands and functional domains; Nuclear domains and structure in the interphase nucleus Chapter 6 Transcription in a Chromatin EnvironmentIntroduction; Genes are packaged into nucleosomes, even when they are being transcribed; Genetic experiments in yeast show the importance of histones for gene regulation; Changes in chromatin structure precede gene activation; Increased histone acetylation can precede or accompany the onset of transcription; DNaseI hypersensitive sites; Nucleosome positioning in vitro and in vivo; Chromatin domains; Chapter 7 How the Transcription Machinery Deals with Chromatin; Introduction; In vitro studies of transcription factor binding A crowded nucleosome: Mouse Mammary Tumour Virus nucleosome BThe opportunities presented by DNA replication; Chromatin and the elongation stage of transcription; Chapter 8 Chromatin Remodelling Machines; Introduction; Nucleosome remodelling enzymes; Histone acetyltransferases (HATs); Histone deacetylases; The nuclear receptors; Chromatin and cancer; Chapter 9 Heterochromatin; Introduction; α and β heterochromatin in Drosophila; Facultative and constitutive heterochromatin; Heterochromatin DNA; Heterochromatin genes; Heterochromatin proteins; Position effect variegation Heterochromatin and gene expression in mammalsChapter 10 Long-term Silencing of Gene Expression; Introduction; DNA methylation; Silencing at telomeres and mating type loci in yeast; Chapter 11 Cellular Memory and Imprinting; Introduction; Maintenance of transcriptional states; Imprinted genes; Chapter 12 Mechanisms of Dosage Compensation; Introduction; Methods of sex determination; Dosage compensation in mammals; Dosage compensation in Drosophila; Dosage compensation in C. elegans; Lessons from dosage compensation; Index |
| Record Nr. | UNINA-9910145414903321 |
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Turner Bryan M
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| Oxford ; ; Malden, MA, : Blackwell Science, 2001 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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