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Foreign DNA in mammalian systems [[electronic resource] /] / Walter Doerfler
| Foreign DNA in mammalian systems [[electronic resource] /] / Walter Doerfler |
| Autore | Doerfler Walter <1933-> |
| Pubbl/distr/stampa | Weinheim ; ; New York, : Wiley-VCH, c2000 |
| Descrizione fisica | 1 online resource (197 p.) |
| Disciplina |
572.819
572.8619 |
| Soggetto topico |
Genetic transformation
Mammals - Genetics Adenoviruses DNA DNA - Methylation |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-76415-9
9786611764159 3-527-61346-3 3-527-61347-1 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Foreign DNA in Mammalian Systems; Preface; Contents; 1 Introduction; 1.1 Further reading; 2 ForeignDNA; 2.1 Foreign DNA in the environment; 2.2 Uptake and modes of persistence of foreign DNA in mammalian cells; 2.3 Further reading; 3 Methods to detect integrated foreign DNA; 3.1 Parameters of foreign DNA integration; 3.2 The fluorescent in situ hybridization method; 3.3 Detection of foreign DNA by Southern DNA ti DNA hybridization; 3.4 Detection of foreign DNA sequences by the pol reaction (PCR); 3.5 Recloning of junction fragments and the detern nucleotide sequences
3.6 Equilibirum sedimentation in alkaline CsCl gral DNA hybridization3.7 Further reading; 4 The adenovirus paradigm; 4.1 An introduction to the adenovirus system; 4.1.1 Clinical background; 4.1.2 Virion structure; 4.1.3 Classification; 4.1.4 Multiplication during productive infection; 4.1.5 Abortive infection; 4.2 An example: Ad12 DNA integration in the Ad12 hamster cell line T637; 4.3 The state of the viral DNA in different cell systems; 4.3.1 Productive infection; 4.3.2 Abortive infection; 4.3.3 Transformation of cells in culture; 4.3.4 Tumor induction by Ad12 in newborn hamsters 4.3.5 Tumor cells in culture4.3.6 Loss of previously integrated Ad12 DNA sequences; 4.4 Adenovirus infection, DNA transfection or DNA microinjection; 4.5 Integration of adenovirus DNA in human cells - significance human somatic gene therapy; 4.6 Studies on integrative recombination of adenovirus DNA in a cell-free system; 4.7 Further reading; 5 Conclusions derived from a survey of junction sites; 5.1 On the characteristics of junction sequences; 5.2 Persistence of integrated foreign DNA - a novel functional type of insertional mutagenesis 5.3 Adenovirus DNA: chromosomal association - covalent genc integration5.4 Further reading; 6 Adenovirus-induced tumor cells and revertants; 6.1 Clonal origin of Ad12-induced tumors; 6.2 Stability - instability; 6.2.1 Hit-and-run mechanism of viral oncogenesis?; 6.2.2 General implications of a hit-and-run mechanism; 6.3 Further reading; 7 Comparisons with other viral systems; 7.1 Integration of viral DNA; 7.2 Transcription of integrated viral genomes; Color plates; 7.3 Virus-induced tumors; 7.4 Replication and integration of the retroviral genome; 7.5 Endogenous retroviral genomes 7.6 The viral archetype: integration of bacteriophage λ DNA7.6.1 The most important regulatory functions in the phage λ genome; 7.6.2 Control of transcription at the right operator OR of phage λ DNA; 7.6.3 A closer look at the integration and excision of the bacteriophage λ genome; 7.7 Further reading; 8 Non-viral systems; 8.1 Exchange of genetic information with extracellular DNA in pneumococci; 8.2 IS elements and transposons; 8.3 Thoughts on the mechanism of foreign DNA integration; 8.4 Expression of integrated foreign DNA; 8.5 Fixation of foreign DNA in transgenic animals 8.6 Critical evaluation of the results obtained with transgenic animals |
| Record Nr. | UNINA-9910144734503321 |
Doerfler Walter <1933->
|
||
| Weinheim ; ; New York, : Wiley-VCH, c2000 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Foreign DNA in mammalian systems [[electronic resource] /] / Walter Doerfler
| Foreign DNA in mammalian systems [[electronic resource] /] / Walter Doerfler |
| Autore | Doerfler Walter <1933-> |
| Pubbl/distr/stampa | Weinheim ; ; New York, : Wiley-VCH, c2000 |
| Descrizione fisica | 1 online resource (197 p.) |
| Disciplina |
572.819
572.8619 |
| Soggetto topico |
Genetic transformation
Mammals - Genetics Adenoviruses DNA DNA - Methylation |
| ISBN |
1-281-76415-9
9786611764159 3-527-61346-3 3-527-61347-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Foreign DNA in Mammalian Systems; Preface; Contents; 1 Introduction; 1.1 Further reading; 2 ForeignDNA; 2.1 Foreign DNA in the environment; 2.2 Uptake and modes of persistence of foreign DNA in mammalian cells; 2.3 Further reading; 3 Methods to detect integrated foreign DNA; 3.1 Parameters of foreign DNA integration; 3.2 The fluorescent in situ hybridization method; 3.3 Detection of foreign DNA by Southern DNA ti DNA hybridization; 3.4 Detection of foreign DNA sequences by the pol reaction (PCR); 3.5 Recloning of junction fragments and the detern nucleotide sequences
3.6 Equilibirum sedimentation in alkaline CsCl gral DNA hybridization3.7 Further reading; 4 The adenovirus paradigm; 4.1 An introduction to the adenovirus system; 4.1.1 Clinical background; 4.1.2 Virion structure; 4.1.3 Classification; 4.1.4 Multiplication during productive infection; 4.1.5 Abortive infection; 4.2 An example: Ad12 DNA integration in the Ad12 hamster cell line T637; 4.3 The state of the viral DNA in different cell systems; 4.3.1 Productive infection; 4.3.2 Abortive infection; 4.3.3 Transformation of cells in culture; 4.3.4 Tumor induction by Ad12 in newborn hamsters 4.3.5 Tumor cells in culture4.3.6 Loss of previously integrated Ad12 DNA sequences; 4.4 Adenovirus infection, DNA transfection or DNA microinjection; 4.5 Integration of adenovirus DNA in human cells - significance human somatic gene therapy; 4.6 Studies on integrative recombination of adenovirus DNA in a cell-free system; 4.7 Further reading; 5 Conclusions derived from a survey of junction sites; 5.1 On the characteristics of junction sequences; 5.2 Persistence of integrated foreign DNA - a novel functional type of insertional mutagenesis 5.3 Adenovirus DNA: chromosomal association - covalent genc integration5.4 Further reading; 6 Adenovirus-induced tumor cells and revertants; 6.1 Clonal origin of Ad12-induced tumors; 6.2 Stability - instability; 6.2.1 Hit-and-run mechanism of viral oncogenesis?; 6.2.2 General implications of a hit-and-run mechanism; 6.3 Further reading; 7 Comparisons with other viral systems; 7.1 Integration of viral DNA; 7.2 Transcription of integrated viral genomes; Color plates; 7.3 Virus-induced tumors; 7.4 Replication and integration of the retroviral genome; 7.5 Endogenous retroviral genomes 7.6 The viral archetype: integration of bacteriophage λ DNA7.6.1 The most important regulatory functions in the phage λ genome; 7.6.2 Control of transcription at the right operator OR of phage λ DNA; 7.6.3 A closer look at the integration and excision of the bacteriophage λ genome; 7.7 Further reading; 8 Non-viral systems; 8.1 Exchange of genetic information with extracellular DNA in pneumococci; 8.2 IS elements and transposons; 8.3 Thoughts on the mechanism of foreign DNA integration; 8.4 Expression of integrated foreign DNA; 8.5 Fixation of foreign DNA in transgenic animals 8.6 Critical evaluation of the results obtained with transgenic animals |
| Record Nr. | UNINA-9910830019203321 |
|
Doerfler Walter <1933->
|
||
| Weinheim ; ; New York, : Wiley-VCH, c2000 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Foreign DNA in mammalian systems / / Walter Doerfler
| Foreign DNA in mammalian systems / / Walter Doerfler |
| Autore | Doerfler Walter <1933-> |
| Pubbl/distr/stampa | Weinheim ; ; New York, : Wiley-VCH, c2000 |
| Descrizione fisica | 1 online resource (197 p.) |
| Disciplina |
572.819
572.8619 |
| Soggetto topico |
Genetic transformation
Mammals - Genetics Adenoviruses DNA DNA - Methylation |
| ISBN |
1-281-76415-9
9786611764159 3-527-61346-3 3-527-61347-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Foreign DNA in Mammalian Systems; Preface; Contents; 1 Introduction; 1.1 Further reading; 2 ForeignDNA; 2.1 Foreign DNA in the environment; 2.2 Uptake and modes of persistence of foreign DNA in mammalian cells; 2.3 Further reading; 3 Methods to detect integrated foreign DNA; 3.1 Parameters of foreign DNA integration; 3.2 The fluorescent in situ hybridization method; 3.3 Detection of foreign DNA by Southern DNA ti DNA hybridization; 3.4 Detection of foreign DNA sequences by the pol reaction (PCR); 3.5 Recloning of junction fragments and the detern nucleotide sequences
3.6 Equilibirum sedimentation in alkaline CsCl gral DNA hybridization3.7 Further reading; 4 The adenovirus paradigm; 4.1 An introduction to the adenovirus system; 4.1.1 Clinical background; 4.1.2 Virion structure; 4.1.3 Classification; 4.1.4 Multiplication during productive infection; 4.1.5 Abortive infection; 4.2 An example: Ad12 DNA integration in the Ad12 hamster cell line T637; 4.3 The state of the viral DNA in different cell systems; 4.3.1 Productive infection; 4.3.2 Abortive infection; 4.3.3 Transformation of cells in culture; 4.3.4 Tumor induction by Ad12 in newborn hamsters 4.3.5 Tumor cells in culture4.3.6 Loss of previously integrated Ad12 DNA sequences; 4.4 Adenovirus infection, DNA transfection or DNA microinjection; 4.5 Integration of adenovirus DNA in human cells - significance human somatic gene therapy; 4.6 Studies on integrative recombination of adenovirus DNA in a cell-free system; 4.7 Further reading; 5 Conclusions derived from a survey of junction sites; 5.1 On the characteristics of junction sequences; 5.2 Persistence of integrated foreign DNA - a novel functional type of insertional mutagenesis 5.3 Adenovirus DNA: chromosomal association - covalent genc integration5.4 Further reading; 6 Adenovirus-induced tumor cells and revertants; 6.1 Clonal origin of Ad12-induced tumors; 6.2 Stability - instability; 6.2.1 Hit-and-run mechanism of viral oncogenesis?; 6.2.2 General implications of a hit-and-run mechanism; 6.3 Further reading; 7 Comparisons with other viral systems; 7.1 Integration of viral DNA; 7.2 Transcription of integrated viral genomes; Color plates; 7.3 Virus-induced tumors; 7.4 Replication and integration of the retroviral genome; 7.5 Endogenous retroviral genomes 7.6 The viral archetype: integration of bacteriophage λ DNA7.6.1 The most important regulatory functions in the phage λ genome; 7.6.2 Control of transcription at the right operator OR of phage λ DNA; 7.6.3 A closer look at the integration and excision of the bacteriophage λ genome; 7.7 Further reading; 8 Non-viral systems; 8.1 Exchange of genetic information with extracellular DNA in pneumococci; 8.2 IS elements and transposons; 8.3 Thoughts on the mechanism of foreign DNA integration; 8.4 Expression of integrated foreign DNA; 8.5 Fixation of foreign DNA in transgenic animals 8.6 Critical evaluation of the results obtained with transgenic animals |
| Record Nr. | UNINA-9910876774203321 |
|
Doerfler Walter <1933->
|
||
| Weinheim ; ; New York, : Wiley-VCH, c2000 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Genome organization and function in the cell nucleus [[electronic resource] /] / edited by Karsten Rippe
| Genome organization and function in the cell nucleus [[electronic resource] /] / edited by Karsten Rippe |
| Pubbl/distr/stampa | Heidelberg, Germany, : Wiley-VCH, c2012 |
| Descrizione fisica | 1 online resource (596 p.) |
| Disciplina | 572.8619 |
| Altri autori (Persone) | RippeKarsten |
| Soggetto topico |
Mammals - Genetics
Genomes Molecular genetics |
| Soggetto genere / forma | Aufsatzsammlung |
| ISBN |
3-527-64000-2
1-283-86990-X 3-527-64001-0 3-527-63999-3 |
| Classificazione |
WE 4000
570 QU 350 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Genome Organization and Function in the Cell Nucleus; Contents; Preface; List of Contributors; 1: Deciphering DNA Sequence Information; 1.1: Introduction; 1.2: Genes and Transcribed Regions; 1.2.1: Open Reading Frames; 1.2.2: Mapping Transcriptional Start Sites; 1.2.3: Mapping Untranslated Regions on mRNA; 1.3: Non-Coding Genomic Elements; 1.3.1: Pseudogenes; 1.3.2: Repeats; 1.3.3: Structural Variants; 1.3.4: Methods for SV Detection; 1.3.5: Transposons and Retrotransposons; 1.4: Regulatory Information; 1.4.1: Classes of Regulatory Elements; 1.4.2: Transcription Factor Binding Motifs
1.4.3: Allele-Specific Expression1.5: Individual Genetic Polymorphisms and Their Effect on Gene Expression; 1.6: Conclusion; 2: DNA Methylation; 2.1: Introduction; 2.1.1: Discovery of 5-Methylcytosine in DNA; 2.1.2: Epigenetic Control of Gene Expression; 2.2: Eukaryotic DNA Methyltransferases; 2.2.1: Dnmt1; 2.2.2: The Dnmt3 Family; 2.2.3: Cooperative Function of Dnmts; 2.3: Distribution of 5-Methylcytosine in the Mammalian Genome; 2.3.1: Spatial Distribution of 5-Methylcytosine; 2.3.2: CpG Islands and Promoter Regulation; 2.3.3: Repetitive DNA Sequences 2.3.4: Temporal Distribution of 5-Methylcytosine2.4: Control of Gene Expression by DNA Methylation; 2.4.1: Loss of Transcription Factor Binding; 2.4.2: Methyl-CpG Binding Proteins; 2.4.3: Interconnection of DNA Methylation with Other Epigenetic Pathways; 2.4.4: DNA Methylation, Higher Order Chromatin Structure, and Nuclear Architecture; 2.5: DNA Demethylation; 3: Nucleosomes as Control Elements for Accessing the Genome; 3.1: Introduction and Basic Terminology; 3.2: Nucleosomes are the Building Blocks of Chromatin; 3.2.1: Histones; 3.2.2: Protein DNA Interactions in the Nucleosome 3.2.3: The Structure of Nucleosomal DNA3.3: Nucleosomes Are Dynamic Macromolecular Assemblies; 3.3.1: Mechanisms to Promote Histone Exchange In Vivo; 3.3.2: In Vitro Determination of Nucleosome Stability; 3.4: Histone Variants and Their Effect on Nucleosome Structure and Dynamics; 3.4.1: Variant Nucleosome Structures; 3.5: Histone Modifications in Nucleosome and Chromatin Structure; 3.6: DNA Sequence and Nucleosome Positioning; 3.6.1: Mechanisms for Nucleosome Exclusion; 3.6.2: Features that Promote Nucleosome Formation; 3.7: Histone Chaperones and Chromatin Dynamics; 3.7.1: Transcription 3.7.2: DNA Replication3.7.3: DNA Repair; 3.7.4: Mechanism of Chaperone-Mediated Nucleosome Assembly and Disassembly; 3.8: Outlook and Concluding Remarks; 4: Histone Modifications and Their Role as Epigenetic Marks; 4.1: The Complexity of Histone Modifications; 4.2: Regulating Histone Modifications in Chromatin; 4.3: The ""Histone Code"" Hypothesis; 4.3.1: Defining Histone ""Marks."" Why Is Histone Modification so Complex?; 4.3.2: Recognizing Histone Modifications: ""Reader"" Domains; 4.4: Exploiting the Complexity of the Histone Code: ""Crosstalk"" Between Different Modifications 4.4.1: Histone ""Crosstalk"": Increased Code Complexity and Signal Integration |
| Record Nr. | UNINA-9910133840103321 |
| Heidelberg, Germany, : Wiley-VCH, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Genome organization and function in the cell nucleus / / edited by Karsten Rippe
| Genome organization and function in the cell nucleus / / edited by Karsten Rippe |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Heidelberg, Germany, : Wiley-VCH, c2012 |
| Descrizione fisica | 1 online resource (596 p.) |
| Disciplina | 572.8619 |
| Altri autori (Persone) | RippeKarsten |
| Soggetto topico |
Mammals - Genetics
Genomes Molecular genetics |
| Soggetto genere / forma | Aufsatzsammlung |
| ISBN |
3-527-64000-2
1-283-86990-X 3-527-64001-0 3-527-63999-3 |
| Classificazione |
WE 4000
570 QU 350 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Genome Organization and Function in the Cell Nucleus; Contents; Preface; List of Contributors; 1: Deciphering DNA Sequence Information; 1.1: Introduction; 1.2: Genes and Transcribed Regions; 1.2.1: Open Reading Frames; 1.2.2: Mapping Transcriptional Start Sites; 1.2.3: Mapping Untranslated Regions on mRNA; 1.3: Non-Coding Genomic Elements; 1.3.1: Pseudogenes; 1.3.2: Repeats; 1.3.3: Structural Variants; 1.3.4: Methods for SV Detection; 1.3.5: Transposons and Retrotransposons; 1.4: Regulatory Information; 1.4.1: Classes of Regulatory Elements; 1.4.2: Transcription Factor Binding Motifs
1.4.3: Allele-Specific Expression1.5: Individual Genetic Polymorphisms and Their Effect on Gene Expression; 1.6: Conclusion; 2: DNA Methylation; 2.1: Introduction; 2.1.1: Discovery of 5-Methylcytosine in DNA; 2.1.2: Epigenetic Control of Gene Expression; 2.2: Eukaryotic DNA Methyltransferases; 2.2.1: Dnmt1; 2.2.2: The Dnmt3 Family; 2.2.3: Cooperative Function of Dnmts; 2.3: Distribution of 5-Methylcytosine in the Mammalian Genome; 2.3.1: Spatial Distribution of 5-Methylcytosine; 2.3.2: CpG Islands and Promoter Regulation; 2.3.3: Repetitive DNA Sequences 2.3.4: Temporal Distribution of 5-Methylcytosine2.4: Control of Gene Expression by DNA Methylation; 2.4.1: Loss of Transcription Factor Binding; 2.4.2: Methyl-CpG Binding Proteins; 2.4.3: Interconnection of DNA Methylation with Other Epigenetic Pathways; 2.4.4: DNA Methylation, Higher Order Chromatin Structure, and Nuclear Architecture; 2.5: DNA Demethylation; 3: Nucleosomes as Control Elements for Accessing the Genome; 3.1: Introduction and Basic Terminology; 3.2: Nucleosomes are the Building Blocks of Chromatin; 3.2.1: Histones; 3.2.2: Protein DNA Interactions in the Nucleosome 3.2.3: The Structure of Nucleosomal DNA3.3: Nucleosomes Are Dynamic Macromolecular Assemblies; 3.3.1: Mechanisms to Promote Histone Exchange In Vivo; 3.3.2: In Vitro Determination of Nucleosome Stability; 3.4: Histone Variants and Their Effect on Nucleosome Structure and Dynamics; 3.4.1: Variant Nucleosome Structures; 3.5: Histone Modifications in Nucleosome and Chromatin Structure; 3.6: DNA Sequence and Nucleosome Positioning; 3.6.1: Mechanisms for Nucleosome Exclusion; 3.6.2: Features that Promote Nucleosome Formation; 3.7: Histone Chaperones and Chromatin Dynamics; 3.7.1: Transcription 3.7.2: DNA Replication3.7.3: DNA Repair; 3.7.4: Mechanism of Chaperone-Mediated Nucleosome Assembly and Disassembly; 3.8: Outlook and Concluding Remarks; 4: Histone Modifications and Their Role as Epigenetic Marks; 4.1: The Complexity of Histone Modifications; 4.2: Regulating Histone Modifications in Chromatin; 4.3: The ""Histone Code"" Hypothesis; 4.3.1: Defining Histone ""Marks."" Why Is Histone Modification so Complex?; 4.3.2: Recognizing Histone Modifications: ""Reader"" Domains; 4.4: Exploiting the Complexity of the Histone Code: ""Crosstalk"" Between Different Modifications 4.4.1: Histone ""Crosstalk"": Increased Code Complexity and Signal Integration |
| Record Nr. | UNINA-9910813135703321 |
| Heidelberg, Germany, : Wiley-VCH, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||