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The aptamer handbook : functional oligonucleotides and their applications / / edited by Sven Klussmann
| The aptamer handbook : functional oligonucleotides and their applications / / edited by Sven Klussmann |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH, c2006 |
| Descrizione fisica | 1 online resource (520 p.) |
| Disciplina | 572.8/5 |
| Altri autori (Persone) | KlussmannSven |
| Soggetto topico | Oligonucleotides |
| ISBN |
1-280-72339-4
9786610723393 3-527-60819-2 3-527-60791-9 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
The Aptamer Handbook; Contents; Preface; Foreword; List of Contributors; Part 1 History and Theoretical Background; 1 In Vitro Selection of Functional Oligonucleotides and the Origins of Biochemical Activity; 1.1 Introduction; 1.2 A Brief History of In Vitro Selection; 1.3 Lessons from the Aptamers, Ribozymes, Deoxyribozymes Generated by In Vitro Selection; 1.4 Synthetic Approaches to Understanding the Natural Origins of Function; 1.5 Recent Technological Developments and Future Directions; 1.6 Conclusion; Acknowledgments; References
2 Mathematical Models on RNA Evolution, Simulations In Silico, and Concepts for In Vitro Selection2.1 From Early Experiments and Theories to the Concept of Neutral Networks; 2.1.1 Evolution in the Test Tube; 2.1.2 Kinetic Theory of the Evolution of Molecules; 2.1.3 Sequence Space and Shape Space; 2.2 RNA Structures, Thermodynamics and Kinetic Folding; 2.2.1 Secondary Structures of Minimum Free Energies; 2.2.2 Inverse Folding; 2.2.3 Suboptimal Conformations and Kinetic Folding; 2.2.4 Cofolding and DNA Parameters; 2.3 Neutral Networks and In Silico Evolution of Molecules 2.3.1 Neutral Networks in Sequence Space2.3.2 RNA Evolution In Silico; 2.3.3 Lessons from Evolution In Silico; 2.4 Designed and Natural RNA Switches; 2.5 Outlook on Future Problems in RNA Design; Acknowledgments; References; 3 Fitness Landscapes, Error Thresholds, and Cofactors in Aptamer Evolution; 3.1 Introduction; 3.2 Functionality Landscapes Inferred from Examples; 3.2.1 Fitness Landscape; 3.2.2 Damage Selection Experiments with Ribozymes; 3.2.3 Construction of the Fitness Landscape; 3.2.3.1 Compatible Structure; 3.2.3.2 Mispairs; 3.2.3.3 Critical Sites; 3.2.3.4 Predicted Structure 3.2.4 Case Study: The Fitness Landscape of the Neurospora VS Ribozyme3.2.4.1 Compatible Structure of the VS Ribozyme; 3.2.4.2 Allowed Mispairs in the VS Ribozyme; 3.2.4.3 Critical Sites in the VS Ribozyme; 3.2.4.4 Predicted Structure for the VS Ribozyme; 3.2.4.5 Properties of the Estimated Fitness Landscape for the VS Ribozyme; 3.3 Error Thresholds Inferred from Functional Landscapes: The "Realistic" Error Threshold of the Neurospora VS Ribozyme; 3.4 Looking for Catalytic Partners: Cofactors and Aptamers; 3.4.1 Co-ribozymes (cofactor-assisted ribozymes); 3.4.2 Aptazymes 3.5 The Use of Coenzymes: From the RNA World to the Protein World via Translation and the Genetic Code3.6 Outlook; Acknowledgments; References; Part 2 In Vitro Selection of Target-binding Oligonucleotides; 4 Aptamers to Small Molecules; 4.1 Introduction; 4.2 Aptamers to Nucleotides/Nucleosides/Nucleobases; 4.3 Aptamers to Cofactors; 4.4 Aptamers to Amino Acids; 4.5 Aptamers to Carbohydrates; 4.6 Aptamers to Natural Products; 4.7 Aptamers to Organic or Fluorescent Dyes; 4.8 The Chimeric Approach for Aptamer Selection; 4.9 Conclusion; Acknowledgments; References; 5 Aptamers to Antibiotics 5.1 Introduction |
| Record Nr. | UNINA-9910876887003321 |
| Weinheim, : Wiley-VCH, c2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Handbook of analysis of oligonucleotides and related products / / edited by Jose V. Bonilla, G. Susan Srivatsa
| Handbook of analysis of oligonucleotides and related products / / edited by Jose V. Bonilla, G. Susan Srivatsa |
| Pubbl/distr/stampa | Boca Raton, Fla. : , : CRC Press, , 2011 |
| Descrizione fisica | 1 online resource (502 p.) |
| Disciplina | 572.8/5 |
| Altri autori (Persone) |
BonillaJose V
SrivatsaSusan |
| Soggetto topico |
Oligonucleotides - Analysis
Biochemistry |
| Soggetto genere / forma | Electronic books. |
| ISBN |
0-429-13666-8
1-283-27446-9 9786613274465 1-4398-1994-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Contents; Preface; Editors; Contributors; Introduction; Chapter 1: Purity Analysis and Impurities Determination by Reversed-Phase High-Performance Liquid Chromatography; Chapter 2: Purity Analysis and Impurities Determination by AEX-HPLC; Chapter 3: Purity Analysis and Molecular Weight Determination by Size Exclusion HPLC; Chapter 4: Analysis of Oligonucleotides by Liquid Chromatography and Mass Spectrometry; Chapter 6: Tm Analysis of Oligonucleotides; Chapter 7: Purity and Content Analysis of Oligonucleotides by Capillary Gel Electrophoresis
Chapter 8: Bioanalysis of Therapeutic Oligonucleotides Using Hybridization-Based Immunoassay TechniquesChapter 9: Oligonucleotide Assay and Potency; Chapter 10: Microbial Analysis : Endotoxin Testing; Chapter 11: Analysis of Residual Solvents by Head Space Gas Chromatography; Chapter 12: Determination of Extinction Coefficient; Chapter 13: Structural Determination by NMR; Chapter 14: Infrared Analysis of Oligonucleotides; Chapter 15: Stability Indicating Methods for Oligonucleotide Products; Chapter 16: Analysis by Hydrophilic Interaction Chromatography Chapter 17: Determination of Base CompositionChapter 19: Regulatory Considerations for the Development of Oligonucleotide Therapeutics; Back Cover |
| Record Nr. | UNINA-9910457752203321 |
| Boca Raton, Fla. : , : CRC Press, , 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Handbook of analysis of oligonucleotides and related products / / edited by Jose V. Bonilla, G. Susan Srivatsa
| Handbook of analysis of oligonucleotides and related products / / edited by Jose V. Bonilla, G. Susan Srivatsa |
| Pubbl/distr/stampa | Boca Raton, Fla. : , : CRC Press, , 2011 |
| Descrizione fisica | 1 online resource (502 p.) |
| Disciplina | 572.8/5 |
| Altri autori (Persone) |
BonillaJose V
SrivatsaSusan |
| Soggetto topico |
Oligonucleotides - Analysis
Biochemistry |
| ISBN |
0-429-13666-8
1-283-27446-9 9786613274465 1-4398-1994-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Contents; Preface; Editors; Contributors; Introduction; Chapter 1: Purity Analysis and Impurities Determination by Reversed-Phase High-Performance Liquid Chromatography; Chapter 2: Purity Analysis and Impurities Determination by AEX-HPLC; Chapter 3: Purity Analysis and Molecular Weight Determination by Size Exclusion HPLC; Chapter 4: Analysis of Oligonucleotides by Liquid Chromatography and Mass Spectrometry; Chapter 6: Tm Analysis of Oligonucleotides; Chapter 7: Purity and Content Analysis of Oligonucleotides by Capillary Gel Electrophoresis
Chapter 8: Bioanalysis of Therapeutic Oligonucleotides Using Hybridization-Based Immunoassay TechniquesChapter 9: Oligonucleotide Assay and Potency; Chapter 10: Microbial Analysis : Endotoxin Testing; Chapter 11: Analysis of Residual Solvents by Head Space Gas Chromatography; Chapter 12: Determination of Extinction Coefficient; Chapter 13: Structural Determination by NMR; Chapter 14: Infrared Analysis of Oligonucleotides; Chapter 15: Stability Indicating Methods for Oligonucleotide Products; Chapter 16: Analysis by Hydrophilic Interaction Chromatography Chapter 17: Determination of Base CompositionChapter 19: Regulatory Considerations for the Development of Oligonucleotide Therapeutics; Back Cover |
| Record Nr. | UNINA-9910781746803321 |
| Boca Raton, Fla. : , : CRC Press, , 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Handbook of analysis of oligonucleotides and related products / / edited by Jose V. Bonilla, G. Susan Srivatsa
| Handbook of analysis of oligonucleotides and related products / / edited by Jose V. Bonilla, G. Susan Srivatsa |
| Pubbl/distr/stampa | Boca Raton, Fla. : , : CRC Press, , 2011 |
| Descrizione fisica | 1 online resource (502 p.) |
| Disciplina | 572.8/5 |
| Altri autori (Persone) |
BonillaJose V
SrivatsaSusan |
| Soggetto topico |
Oligonucleotides - Analysis
Biochemistry |
| ISBN |
0-429-13666-8
1-283-27446-9 9786613274465 1-4398-1994-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Contents; Preface; Editors; Contributors; Introduction; Chapter 1: Purity Analysis and Impurities Determination by Reversed-Phase High-Performance Liquid Chromatography; Chapter 2: Purity Analysis and Impurities Determination by AEX-HPLC; Chapter 3: Purity Analysis and Molecular Weight Determination by Size Exclusion HPLC; Chapter 4: Analysis of Oligonucleotides by Liquid Chromatography and Mass Spectrometry; Chapter 6: Tm Analysis of Oligonucleotides; Chapter 7: Purity and Content Analysis of Oligonucleotides by Capillary Gel Electrophoresis
Chapter 8: Bioanalysis of Therapeutic Oligonucleotides Using Hybridization-Based Immunoassay TechniquesChapter 9: Oligonucleotide Assay and Potency; Chapter 10: Microbial Analysis : Endotoxin Testing; Chapter 11: Analysis of Residual Solvents by Head Space Gas Chromatography; Chapter 12: Determination of Extinction Coefficient; Chapter 13: Structural Determination by NMR; Chapter 14: Infrared Analysis of Oligonucleotides; Chapter 15: Stability Indicating Methods for Oligonucleotide Products; Chapter 16: Analysis by Hydrophilic Interaction Chromatography Chapter 17: Determination of Base CompositionChapter 19: Regulatory Considerations for the Development of Oligonucleotide Therapeutics; Back Cover |
| Record Nr. | UNINA-9910800078703321 |
| Boca Raton, Fla. : , : CRC Press, , 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Handbook of analysis of oligonucleotides and related products / / edited by Jose V. Bonilla, Susan Srivatsa
| Handbook of analysis of oligonucleotides and related products / / edited by Jose V. Bonilla, Susan Srivatsa |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Boca Raton, FL, : CRC Press, 2011 |
| Descrizione fisica | 1 online resource (502 p.) |
| Disciplina | 572.8/5 |
| Altri autori (Persone) |
BonillaJose V
SrivatsaG. Susan |
| Soggetto topico |
Oligonucleotides - Analysis
Biochemistry |
| ISBN |
0-429-13666-8
1-283-27446-9 9786613274465 1-4398-1994-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Contents; Preface; Editors; Contributors; Introduction; Chapter 1: Purity Analysis and Impurities Determination by Reversed-Phase High-Performance Liquid Chromatography; Chapter 2: Purity Analysis and Impurities Determination by AEX-HPLC; Chapter 3: Purity Analysis and Molecular Weight Determination by Size Exclusion HPLC; Chapter 4: Analysis of Oligonucleotides by Liquid Chromatography and Mass Spectrometry; Chapter 6: Tm Analysis of Oligonucleotides; Chapter 7: Purity and Content Analysis of Oligonucleotides by Capillary Gel Electrophoresis
Chapter 8: Bioanalysis of Therapeutic Oligonucleotides Using Hybridization-Based Immunoassay TechniquesChapter 9: Oligonucleotide Assay and Potency; Chapter 10: Microbial Analysis : Endotoxin Testing; Chapter 11: Analysis of Residual Solvents by Head Space Gas Chromatography; Chapter 12: Determination of Extinction Coefficient; Chapter 13: Structural Determination by NMR; Chapter 14: Infrared Analysis of Oligonucleotides; Chapter 15: Stability Indicating Methods for Oligonucleotide Products; Chapter 16: Analysis by Hydrophilic Interaction Chromatography Chapter 17: Determination of Base CompositionChapter 19: Regulatory Considerations for the Development of Oligonucleotide Therapeutics; Back Cover |
| Record Nr. | UNINA-9910812821503321 |
| Boca Raton, FL, : CRC Press, 2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Oligonucleotide-based drugs and therapeutics : preclinical and clinical considerations for development / / edited by Nicolay Ferrari, Rosanne Seguin
| Oligonucleotide-based drugs and therapeutics : preclinical and clinical considerations for development / / edited by Nicolay Ferrari, Rosanne Seguin |
| Pubbl/distr/stampa | John Wiley & Sons, Inc |
| Disciplina | 572.8/5 |
| Soggetto topico |
Oligonucleotides - Therapeutic use
Antisense nucleic acids - Therapeutic use |
| ISBN |
1-119-07030-9
1-119-07029-5 1-119-07015-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Mechanisms of oligonucleotide actions -- The medicinal chemistry of antisense oligonucleotides -- Cellular pharmacology of antisense oligonucleotides -- Pharmacokinetics and pharmacodynamics of antisense oligonucleotides -- Tissue distribution, metabolism and clearance -- Hybridization-independent effects : principles and specific considerations for oligonucleotide drugs -- Hybridization-dependent effects : the prediction, evaluation and consequences of unintended target hybridization -- Class-related proinflammatory effects -- Exaggerated pharmacology -- Genotoxicity tests for novel oligonucleotide-based therapeutics -- Reproductive and developmental toxicity testing strategies for oligonucleotide-based therapeutics -- Specific considerations for preclinical development of inhaled oligonucleotides -- Lessons learned in oncology programs -- Inhaled antisense for treatment of respiratory disease -- Antisense oligonucleotides for treatment of neurology diseases -- Nucleic acids as adjuvants -- Splice-switching oligonucleotides -- CMC aspects for the clinical development of spiegelmers. |
| Record Nr. | UNINA-9910555194803321 |
| John Wiley & Sons, Inc | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Oligonucleotide-based drugs and therapeutics : preclinical and clinical considerations for development / / edited by Nicolay Ferrari, Rosanne Seguin
| Oligonucleotide-based drugs and therapeutics : preclinical and clinical considerations for development / / edited by Nicolay Ferrari, Rosanne Seguin |
| Pubbl/distr/stampa | John Wiley & Sons, Inc |
| Disciplina | 572.8/5 |
| Soggetto topico |
Oligonucleotides - Therapeutic use
Antisense nucleic acids - Therapeutic use |
| ISBN |
1-119-07030-9
1-119-07029-5 1-119-07015-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Mechanisms of oligonucleotide actions -- The medicinal chemistry of antisense oligonucleotides -- Cellular pharmacology of antisense oligonucleotides -- Pharmacokinetics and pharmacodynamics of antisense oligonucleotides -- Tissue distribution, metabolism and clearance -- Hybridization-independent effects : principles and specific considerations for oligonucleotide drugs -- Hybridization-dependent effects : the prediction, evaluation and consequences of unintended target hybridization -- Class-related proinflammatory effects -- Exaggerated pharmacology -- Genotoxicity tests for novel oligonucleotide-based therapeutics -- Reproductive and developmental toxicity testing strategies for oligonucleotide-based therapeutics -- Specific considerations for preclinical development of inhaled oligonucleotides -- Lessons learned in oncology programs -- Inhaled antisense for treatment of respiratory disease -- Antisense oligonucleotides for treatment of neurology diseases -- Nucleic acids as adjuvants -- Splice-switching oligonucleotides -- CMC aspects for the clinical development of spiegelmers. |
| Record Nr. | UNINA-9910830042603321 |
| John Wiley & Sons, Inc | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Protein sequencing and identification using tandem mass spectrometry / / Michael Kinter, Nicholas E. Sherman
| Protein sequencing and identification using tandem mass spectrometry / / Michael Kinter, Nicholas E. Sherman |
| Autore | Kinter Michael |
| Pubbl/distr/stampa | New York, : Wiley-Interscience, c2000 |
| Descrizione fisica | 1 online resource (321 p.) |
| Disciplina | 572.8/5 |
| Altri autori (Persone) | ShermanNicholas E |
| Collana | Wiley-Interscience series on mass spectrometry |
| Soggetto topico |
Nucleotide sequence
Proteins Mass spectrometry |
| ISBN |
1-280-54166-0
9786610541669 0-471-23188-6 0-471-72198-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
PROTEIN SEQUENCING AND IDENTIFICATION USING TANDEM MASS SPECTROMETRY; CONTENTS; Series Preface; Preface; Chapter 1. An Introduction to Protein Sequencing Using Tandem Mass Spectrometry; 1.1. Introduction; 1.2. References; Chapter 2. The Primary Structure of Proteins and a Historical Overview of Protein Sequencing; 2.1. Protein and Peptide Structure; 2.2. Edman Degradation; 2.2.1. The Edman Reaction; 2.2.2. Incorporation of the Edman Degradation Reaction into Automated Protein Sequenators; 2.2.3. Edman Degradation in Proteomic Research; 2.3. Tandem Mass Spectrometry
2.3.1. A Brief History of the Application of Mass Spectrometry to Protein Sequencing2.3.2. Sequence Analysis of Peptides Using Electron Ionization Mass Spectrometry; 2.3.3. The Utilization of Fast Atom Bombardment with Tandem Mass Spectrometry to Sequence Peptides; 2.3.4. Internal Sequence Analysis of Proteins Using Electrospray Ionization-Tandem Mass Spectrometry and Matrix-Assisted Laser Desorption/Ionization-Time-of- Flight Mass Spectrometry; 2.4. Summary; 2.5. References; Chapter 3. Fundamental Mass Spectrometry; 3.1. An Overview of the Instrumentation; 3.2. Ionization Methods 3.2.1. Electrospray Ionization3.2.2. Nanospray and Microspray Ionization; 3.2.3. Matrix-Assisted Laser Desorption/ Ionization; 3.3. Mass Analyzers; 3.3.1. Fundamental Parameters of Mass Analysis; 3.3.2. Quadrupole Mass Filters (3.20); 3.3.3. Ion Trap Mass Analyzers (3.21-3.23); 3.3.4. Time-of-Flight Mass Analyzers (3.5); 3.4. Tandem Mass Spectrometry; 3.4.1. Collisionally Induced Dissociation; 3.4.2. Tandem Mass Spectrometers; 3.4.3. Types of Tandem Mass Spectrometry Experiments; 3.5. Data Systems; 3.6. Summary; 3.7. References Chapter 4. Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra4.1. Introduction; 4.2. Peptide Fragmentation Chemistry; 4.2.1. Collisionally Induced Dissociation of Peptide Ions Formed by Electrospray Ionization; 4.2.2. Fragmentation of Protonated Peptide Ions Formed by Matrix-Assisted Laser Desorption/Ionization; 4.3. Interpretation of the Product Ion Spectra of Tryptic Peptides; 4.3.1. Tabulated Values Used in the Interpretation; 4.3.2. A Strategy for the Interpretation of Product Ion Spectra of Tryptic Peptides 4.3.3. Sample Interpretation Problem Number One4.3.4. Sample Interpretation Problem Number Two; 4.3.5. A Summary of Interpretation Problems One and Two; 4.3.6. Examples of More Difficult Product Ion Spectra That Cannot Be Completely Interpreted; 4.3.7. Interpretation of Product Ion Spectra from Triply Charged Ions; 4.4. Summary; 4.5. References; Chapter 5. Basic Polyacrylamide Gel Electrophoresis; 5.1. Introduction; 5.2. The Principles of Gel Electrophoresis; 5.2.1. Protein Movement and Separation; 5.2.2. Protein Detection 5.3. The Basic Steps in a Polyacrylamide Gel Electrophoresis Experiment |
| Record Nr. | UNINA-9910877595903321 |
Kinter Michael
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| New York, : Wiley-Interscience, c2000 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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