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Molecular biology techniques [[electronic resource] ] : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow
| Molecular biology techniques [[electronic resource] ] : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow |
| Autore | Carson Sue, Dr. |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 |
| Descrizione fisica | 1 online resource (xxvi, 200 pages) : color illustrations |
| Disciplina |
572.8/078
572.8078 |
| Altri autori (Persone) |
MillerHeather Bennett
WitherowD. Scott (Dennis Scott) |
| Soggetto topico |
Molecular biology
Molecular biology - Technique |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-283-31097-X
9786613310972 0-12-385545-4 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. |
| Record Nr. | UNINA-9910457771203321 |
Carson Sue, Dr.
|
||
| Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology techniques [[electronic resource] ] : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow
| Molecular biology techniques [[electronic resource] ] : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow |
| Autore | Carson Sue, Dr. |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 |
| Descrizione fisica | 1 online resource (xxvi, 200 pages) : color illustrations |
| Disciplina |
572.8/078
572.8078 |
| Altri autori (Persone) |
MillerHeather B
WitherowD. Scott (Dennis Scott) |
| Soggetto topico |
Molecular biology
Molecular biology - Technique |
| ISBN |
1-283-31097-X
9786613310972 0-12-385545-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. |
| Record Nr. | UNINA-9910781937603321 |
|
Carson Sue, Dr.
|
||
| Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology techniques : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow
| Molecular biology techniques : a classroom laboratory manual / / Susan Carson, Heather Miller, D. Scott Witherow |
| Autore | Carson Sue (Susan) |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 |
| Descrizione fisica | 1 online resource (xxvi, 200 pages) : color illustrations |
| Disciplina |
572.8/078
572.8078 |
| Altri autori (Persone) |
MillerHeather B
WitherowD. Scott |
| Soggetto topico |
Molecular biology
Molecular biology - Technique |
| ISBN |
9786613310972
9781283310970 128331097X 9780123855459 0123855454 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. |
| Record Nr. | UNINA-9910957092703321 |
|
Carson Sue (Susan)
|
||
| Amsterdam ; ; Boston, : Elsevier /Academic Press, c2012 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology techniques [[electronic resource] ] : an intensive laboratory course / / [edited by] Walt Ream and Katharine G. Field
| Molecular biology techniques [[electronic resource] ] : an intensive laboratory course / / [edited by] Walt Ream and Katharine G. Field |
| Pubbl/distr/stampa | San Diego, CA, : Academic Press, 1998 |
| Descrizione fisica | 1 online resource (245 p.) |
| Disciplina | 572.8/078 |
| Altri autori (Persone) |
FieldKatharine G
ReamWalt |
| Soggetto topico |
Molecular biology
Molecular genetics |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-01889-9
9786611018894 0-08-053682-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Biology Techniques: An Intensive Laboratory Course; Copyright Page; Contents; Preface; Course Synopsis; Introduction; Safety Precautions; Daily Schedule; Acknowledgments; Exercises I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning; A. Cesium chloride-ethidium bromide density gradient centrifugation; B. PCR to synthesize virD2 flanked with restriction sites; C. Restriction digests of plasmid pGEX2 and PCR products; D. Purification of DNA fragments from agarose; E. Ligation of PCR product to pGEX2 vector
F. Transformation of E. coli with the ligated plasmidG. Small-scale preparation of plasmid DNA by the alkaline lysis method; H. Restriction analysis; Study questions; Exercises II. Protein Expression, Purification, and Analysis; A. Expression and purification of a fusion protein; B. SDS-polyacrylamide gel electrophoresis; C. Silver stain detection of proteins; D. Western blot (immunoblot) detection of proteins; Study questions; Exercises III. Oligonucleotide-Directed Mutagenesis; A. Restriction digests of virD2 (in pCS64) and pUC119; B. Purification of DNA fragments from agarose C. Ligation of restriction fragment and vectorD. Transformation of E. coli with the ligated plasmid and recovery of clones; E. Small-scale preparation of plasmid DNA from broth cultures; F. Restriction digest of DNAs: Examination to confirm insert; G. Preparation of single-stranded DNA template; H. Phosphorylation of oligonucleotide; I. Annealing mutant oligonucleotide to template; J. In vitro DNA synthesis by primer extension; K. Transform synthesis reaction into E. coli DH5a; L. Small-scale preparation of plasmid DNA; M. Confirmation of mutants by restriction analysis; Study questions Exercises IV. DNA SequencingA. Polyacrylamide sequencing gel electrophoresis; B. Dideoxy sequencing; C. Automated sequencing; D. Introduction to databases and gene sequence analysis; Study questions; Exercises V. Southern Blot Detection of DNA; A. Preparation of genomic DNA from Agrobacterium tumefaciens; B. Restriction digestion of genomic DNA; C. Agarose gel electrophoresis of restriction fragments; D. Southern blot: Denaturation and blotting of DNA; E. Preparation of probe by nick translation; F. Hybridization and washing of Southern blots; Study questions Exercises VI. Northern Blot Detection of mRNAA. Preparation of RNA from tobacco leaves; B. Agarose-formaldehyde gel electrophoresis; C. Northern blot: Denaturation and blotting of RNA; D. Probe preparation; E. Hybridization and washing of Northern blots; Study questions; Exercises VII. Protein Interaction Analysis in Yeast; A. Yeast transformation; B. Filter ß-galactosidase assay; Index |
| Record Nr. | UNINA-9910458228103321 |
| San Diego, CA, : Academic Press, 1998 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular biology techniques [[electronic resource] ] : an intensive laboratory course / / [edited by] Walt Ream and Katharine G. Field
| Molecular biology techniques [[electronic resource] ] : an intensive laboratory course / / [edited by] Walt Ream and Katharine G. Field |
| Pubbl/distr/stampa | San Diego, CA, : Academic Press, 1998 |
| Descrizione fisica | 1 online resource (245 p.) |
| Disciplina | 572.8/078 |
| Altri autori (Persone) |
FieldKatharine G
ReamWalt |
| Soggetto topico |
Molecular biology
Molecular genetics |
| ISBN |
1-281-01889-9
9786611018894 0-08-053682-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Biology Techniques: An Intensive Laboratory Course; Copyright Page; Contents; Preface; Course Synopsis; Introduction; Safety Precautions; Daily Schedule; Acknowledgments; Exercises I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning; A. Cesium chloride-ethidium bromide density gradient centrifugation; B. PCR to synthesize virD2 flanked with restriction sites; C. Restriction digests of plasmid pGEX2 and PCR products; D. Purification of DNA fragments from agarose; E. Ligation of PCR product to pGEX2 vector
F. Transformation of E. coli with the ligated plasmidG. Small-scale preparation of plasmid DNA by the alkaline lysis method; H. Restriction analysis; Study questions; Exercises II. Protein Expression, Purification, and Analysis; A. Expression and purification of a fusion protein; B. SDS-polyacrylamide gel electrophoresis; C. Silver stain detection of proteins; D. Western blot (immunoblot) detection of proteins; Study questions; Exercises III. Oligonucleotide-Directed Mutagenesis; A. Restriction digests of virD2 (in pCS64) and pUC119; B. Purification of DNA fragments from agarose C. Ligation of restriction fragment and vectorD. Transformation of E. coli with the ligated plasmid and recovery of clones; E. Small-scale preparation of plasmid DNA from broth cultures; F. Restriction digest of DNAs: Examination to confirm insert; G. Preparation of single-stranded DNA template; H. Phosphorylation of oligonucleotide; I. Annealing mutant oligonucleotide to template; J. In vitro DNA synthesis by primer extension; K. Transform synthesis reaction into E. coli DH5a; L. Small-scale preparation of plasmid DNA; M. Confirmation of mutants by restriction analysis; Study questions Exercises IV. DNA SequencingA. Polyacrylamide sequencing gel electrophoresis; B. Dideoxy sequencing; C. Automated sequencing; D. Introduction to databases and gene sequence analysis; Study questions; Exercises V. Southern Blot Detection of DNA; A. Preparation of genomic DNA from Agrobacterium tumefaciens; B. Restriction digestion of genomic DNA; C. Agarose gel electrophoresis of restriction fragments; D. Southern blot: Denaturation and blotting of DNA; E. Preparation of probe by nick translation; F. Hybridization and washing of Southern blots; Study questions Exercises VI. Northern Blot Detection of mRNAA. Preparation of RNA from tobacco leaves; B. Agarose-formaldehyde gel electrophoresis; C. Northern blot: Denaturation and blotting of RNA; D. Probe preparation; E. Hybridization and washing of Northern blots; Study questions; Exercises VII. Protein Interaction Analysis in Yeast; A. Yeast transformation; B. Filter ß-galactosidase assay; Index |
| Record Nr. | UNINA-9910784526803321 |
| San Diego, CA, : Academic Press, 1998 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||