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Calculations in molecular biology and biotechnology [[electronic resource] ] : a guide to mathematics in the laboratory / / Frank H. Stephenson
| Calculations in molecular biology and biotechnology [[electronic resource] ] : a guide to mathematics in the laboratory / / Frank H. Stephenson |
| Autore | Stephenson Frank Harold |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Academic Press, c2003 |
| Descrizione fisica | 1 online resource (319 p.) |
| Disciplina | 572.8/01/51 |
| Soggetto topico |
Molecular biology - Computer simulation
Molecular biology - Mathematics Biotechnology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-02727-8
9786611027278 0-08-050061-7 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Calculations in Molecular Biology and Biotechnology; Copyright Page; Contents; Foreword; Chapter 1. Scientific Notation and Metric Prefixes; Introduction; Significant Digits; Rounding Off Significant Digits in Calculations; Exponents and Scientific Notation; Expressing Numbers in Scientific Notation; Converting Numbers from Scientific Notation to Decimal Notation; Adding and Subtracting Numbers Written in Scientific Notation; Multiplying and Dividing Numbers Written in Scientific Notation; Metric Prefixes; Conversion Factors and Canceling Terms
Chapter 2. Solutions Mixtures and Media Introduction; Calculating Dilutions: A General Approach; Concentrations by a Factor of X; Preparing Percent Solutions; Diluting Percent Solutions; Moles and Molecular Weight: Definitions; Molarity; Diluting Molar Solutions; Converting Molarity to Percent; Converting Percent to Molarity; Normality; PH; pKa and the Henderson-Hasselbalch Equation; Chapter 3. Cell Growth; The Bacterial Growth Curve; Manipulating Cell Concentration; Plotting OD550 vs. Time on a Linear Graph; Plotting the Logarithm of OD550 vs. Time on a Linear Graph Plotting the Log of Cell Concentration vs. Time Calculating Generation Time; Plotting Cell Growth Data on a Semilog Graph; Determining Generation Time Directly from a Semilog Plot of Cell Concentration vs. Time; Plotting Cell Density versus OD550 on a Semilog Graph; The Fluctuation Test; Fluctuation Test Example; Variance; Measuring Mutation Rate; Measuring Cell Concentration on a Hemocytometer; Chapter 4. Working with Bacteriophage; Introduction; Multiplicity of Infection; Probabilities and Multiplicity of Infection; Measuring Phage Titer; Diluting Bacteriophage; Measuring Burst Size Chapter 5. Quantitation of Nucleic Acids Quantitation of Nucleic Acids by Ultraviolet Spectroscopy; Determining the Concentration of Double-Stranded DNA; Using Absorbance and an Extinction Coefficient to Calculate Double-Stranded DNA Concentration; Calculating DNA Concentration as a Millimolar (mM) Amount; Determining the Concentration of Single-Stranded DNA Molecules; Oligonucleotide Quantitation; Measuring RNA Concentration; Molecular Weight, Molarity, and Nucleic Acid Length; Estimating DNA Concentration on an Ethidium Bromide-Stained Gel Chapter 6. Labeling Nucleic Acids with Radioisotopes Introduction; Using Radioactivity: The Curie; Estimating Plasmid Copy Number; Labeling DNA by Nick Translation; Random Primer Labeling of DNA; Labeling 3' Termini with Terminal Transferase; cDNA Synthesis; Homopolymeric Tailing; In Vitro Transcription; Chapter 7. Oligonucleotide Synthesis; Introduction; Synthesis Yield; Measuring Stepwise and Overall Yield by the DMT Cation Assay; Overall Yield; Stepwise Yield; Calculating Micromoles of Nucleoside Added at Each Base Addition Step; Chapter 8. The Polymerase Chain Reaction; Introduction Template and Amplification |
| Record Nr. | UNINA-9910458136503321 |
Stephenson Frank Harold
|
||
| Amsterdam ; ; Boston, : Academic Press, c2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Calculations in molecular biology and biotechnology [[electronic resource] ] : a guide to mathematics in the laboratory / / Frank H. Stephenson
| Calculations in molecular biology and biotechnology [[electronic resource] ] : a guide to mathematics in the laboratory / / Frank H. Stephenson |
| Autore | Stephenson Frank Harold |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Academic Press, c2003 |
| Descrizione fisica | 1 online resource (319 p.) |
| Disciplina | 572.8/01/51 |
| Soggetto topico |
Molecular biology - Computer simulation
Molecular biology - Mathematics Biotechnology |
| ISBN |
1-281-02727-8
9786611027278 0-08-050061-7 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Calculations in Molecular Biology and Biotechnology; Copyright Page; Contents; Foreword; Chapter 1. Scientific Notation and Metric Prefixes; Introduction; Significant Digits; Rounding Off Significant Digits in Calculations; Exponents and Scientific Notation; Expressing Numbers in Scientific Notation; Converting Numbers from Scientific Notation to Decimal Notation; Adding and Subtracting Numbers Written in Scientific Notation; Multiplying and Dividing Numbers Written in Scientific Notation; Metric Prefixes; Conversion Factors and Canceling Terms
Chapter 2. Solutions Mixtures and Media Introduction; Calculating Dilutions: A General Approach; Concentrations by a Factor of X; Preparing Percent Solutions; Diluting Percent Solutions; Moles and Molecular Weight: Definitions; Molarity; Diluting Molar Solutions; Converting Molarity to Percent; Converting Percent to Molarity; Normality; PH; pKa and the Henderson-Hasselbalch Equation; Chapter 3. Cell Growth; The Bacterial Growth Curve; Manipulating Cell Concentration; Plotting OD550 vs. Time on a Linear Graph; Plotting the Logarithm of OD550 vs. Time on a Linear Graph Plotting the Log of Cell Concentration vs. Time Calculating Generation Time; Plotting Cell Growth Data on a Semilog Graph; Determining Generation Time Directly from a Semilog Plot of Cell Concentration vs. Time; Plotting Cell Density versus OD550 on a Semilog Graph; The Fluctuation Test; Fluctuation Test Example; Variance; Measuring Mutation Rate; Measuring Cell Concentration on a Hemocytometer; Chapter 4. Working with Bacteriophage; Introduction; Multiplicity of Infection; Probabilities and Multiplicity of Infection; Measuring Phage Titer; Diluting Bacteriophage; Measuring Burst Size Chapter 5. Quantitation of Nucleic Acids Quantitation of Nucleic Acids by Ultraviolet Spectroscopy; Determining the Concentration of Double-Stranded DNA; Using Absorbance and an Extinction Coefficient to Calculate Double-Stranded DNA Concentration; Calculating DNA Concentration as a Millimolar (mM) Amount; Determining the Concentration of Single-Stranded DNA Molecules; Oligonucleotide Quantitation; Measuring RNA Concentration; Molecular Weight, Molarity, and Nucleic Acid Length; Estimating DNA Concentration on an Ethidium Bromide-Stained Gel Chapter 6. Labeling Nucleic Acids with Radioisotopes Introduction; Using Radioactivity: The Curie; Estimating Plasmid Copy Number; Labeling DNA by Nick Translation; Random Primer Labeling of DNA; Labeling 3' Termini with Terminal Transferase; cDNA Synthesis; Homopolymeric Tailing; In Vitro Transcription; Chapter 7. Oligonucleotide Synthesis; Introduction; Synthesis Yield; Measuring Stepwise and Overall Yield by the DMT Cation Assay; Overall Yield; Stepwise Yield; Calculating Micromoles of Nucleoside Added at Each Base Addition Step; Chapter 8. The Polymerase Chain Reaction; Introduction Template and Amplification |
| Record Nr. | UNINA-9910784562903321 |
|
Stephenson Frank Harold
|
||
| Amsterdam ; ; Boston, : Academic Press, c2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Computational Methods for SNPs and Haplotype Inference [[electronic resource] ] : DIMACS/RECOMB Satellite Workshop, Piscataway, NJ, USA, November 21-22, 2002, Revised Papers / / edited by Sorin Istrail, Michael Waterman, Andrew Clark
| Computational Methods for SNPs and Haplotype Inference [[electronic resource] ] : DIMACS/RECOMB Satellite Workshop, Piscataway, NJ, USA, November 21-22, 2002, Revised Papers / / edited by Sorin Istrail, Michael Waterman, Andrew Clark |
| Edizione | [1st ed. 2004.] |
| Pubbl/distr/stampa | Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2004 |
| Descrizione fisica | 1 online resource (X, 158 p.) |
| Disciplina | 572.8/01/51 |
| Collana | Lecture Notes in Bioinformatics |
| Soggetto topico |
Biochemistry
Algorithms Human genetics Computer science—Mathematics Mathematical statistics Database management Biochemistry, general Algorithm Analysis and Problem Complexity Human Genetics Discrete Mathematics in Computer Science Probability and Statistics in Computer Science Database Management |
| ISBN |
1-280-30709-9
9786610307098 3-540-24719-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Trisomic Phase Inference -- Trisomic Phase Inference -- An Overview of Combinatorial Methods for Haplotype Inference -- A Survey of Computational Methods for Determining Haplotypes -- Haplotype Inference and Its Application in Linkage Disequilibrium Mapping -- Inferring Piecewise Ancestral History from Haploid Sequences -- Haplotype Blocks in Small Populations -- Simulating a Coalescent Process with Recombination and Ascertainment -- Dynamic Programming Algorithms for Haplotype Block Partitioning and Tag SNP Selection Using Haplotype Data or Genotype Data -- Parametric Bootstrap for Assessment of Goodness of Fit of Models for Block Haplotype Structure -- A Coalescent-Based Approach for Complex Disease Mapping -- Abstracts -- Haplotyping as Perfect Phylogeny -- Exhaustive Enumeration and Bayesian Phase Inference -- How Does Choice of Polymorphism Influence Estimation of LD and Mapping? -- Haplotype Inference in Random Population Samples -- Bayesian Methods for Statistical Reconstruction of Haplotypes -- Combinatorial Approaches to Haplotype Inference -- Large Scale Recovery of Haplotypes from Genotype Data Using Imperfect Phylogeny -- Haplotype Inference and Haplotype Information -- Multi-locus Linkage Disequilibrium and Haplotype-Based Tests of Association -- The Pattern of Polymorphism on Human Chromosome 21 -- Use of a Local Approximation to the Ancestral Recombination Graph for Fine Mapping Disease Genes -- Insights into Recombination from Patterns of Linkage Disequilibrium -- Joint Bayesian Estimation of Mutation Location and Age Using Linkage Disequilibrium -- Evolutionary-Based Association Analysis Using Haplotype Data -- Inferring Piecewise Ancestral History from Haploid Sequences -- Testing for Differences in Haplotype Frequencies in Case-Control Studies -- Haplotypes, Hotspots, and a Multilocus Model for Linkage Disequilibrium -- Dynamic Programming Algorithms for Haplotype Block Partition and Applications to Association Studies -- Genome Sharing in Small Populations -- Patterns of Linkage Disequilibrium across Human Chromosomes 6, 21, AND 22 -- A Software System for Automated and Visual Analysis of Functionally Annotated Haplotypes -- Assessment of Goodness of Fit of Models for Block Haplotype Structure. |
| Record Nr. | UNISA-996465537503316 |
| Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Computational Methods for SNPs and Haplotype Inference : DIMACS/RECOMB Satellite Workshop, Piscataway, NJ, USA, November 21-22, 2002, Revised Papers / / edited by Sorin Istrail, Michael Waterman, Andrew Clark
| Computational Methods for SNPs and Haplotype Inference : DIMACS/RECOMB Satellite Workshop, Piscataway, NJ, USA, November 21-22, 2002, Revised Papers / / edited by Sorin Istrail, Michael Waterman, Andrew Clark |
| Edizione | [1st ed. 2004.] |
| Pubbl/distr/stampa | Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2004 |
| Descrizione fisica | 1 online resource (X, 158 p.) |
| Disciplina | 572.8/01/51 |
| Collana | Lecture Notes in Bioinformatics |
| Soggetto topico |
Biochemistry
Algorithms Human genetics Computer science—Mathematics Mathematical statistics Database management Biochemistry, general Algorithm Analysis and Problem Complexity Human Genetics Discrete Mathematics in Computer Science Probability and Statistics in Computer Science Database Management |
| ISBN |
1-280-30709-9
9786610307098 3-540-24719-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Trisomic Phase Inference -- Trisomic Phase Inference -- An Overview of Combinatorial Methods for Haplotype Inference -- A Survey of Computational Methods for Determining Haplotypes -- Haplotype Inference and Its Application in Linkage Disequilibrium Mapping -- Inferring Piecewise Ancestral History from Haploid Sequences -- Haplotype Blocks in Small Populations -- Simulating a Coalescent Process with Recombination and Ascertainment -- Dynamic Programming Algorithms for Haplotype Block Partitioning and Tag SNP Selection Using Haplotype Data or Genotype Data -- Parametric Bootstrap for Assessment of Goodness of Fit of Models for Block Haplotype Structure -- A Coalescent-Based Approach for Complex Disease Mapping -- Abstracts -- Haplotyping as Perfect Phylogeny -- Exhaustive Enumeration and Bayesian Phase Inference -- How Does Choice of Polymorphism Influence Estimation of LD and Mapping? -- Haplotype Inference in Random Population Samples -- Bayesian Methods for Statistical Reconstruction of Haplotypes -- Combinatorial Approaches to Haplotype Inference -- Large Scale Recovery of Haplotypes from Genotype Data Using Imperfect Phylogeny -- Haplotype Inference and Haplotype Information -- Multi-locus Linkage Disequilibrium and Haplotype-Based Tests of Association -- The Pattern of Polymorphism on Human Chromosome 21 -- Use of a Local Approximation to the Ancestral Recombination Graph for Fine Mapping Disease Genes -- Insights into Recombination from Patterns of Linkage Disequilibrium -- Joint Bayesian Estimation of Mutation Location and Age Using Linkage Disequilibrium -- Evolutionary-Based Association Analysis Using Haplotype Data -- Inferring Piecewise Ancestral History from Haploid Sequences -- Testing for Differences in Haplotype Frequencies in Case-Control Studies -- Haplotypes, Hotspots, and a Multilocus Model for Linkage Disequilibrium -- Dynamic Programming Algorithms for Haplotype Block Partition and Applications to Association Studies -- Genome Sharing in Small Populations -- Patterns of Linkage Disequilibrium across Human Chromosomes 6, 21, AND 22 -- A Software System for Automated and Visual Analysis of Functionally Annotated Haplotypes -- Assessment of Goodness of Fit of Models for Block Haplotype Structure. |
| Record Nr. | UNINA-9910144204103321 |
| Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||