The art of carbohydrate analysis / / Gerrit J. Gerwig |
Autore | Gerwig Gerrit J. |
Pubbl/distr/stampa | Cham, Switzerland : , : Springer, , [2021] |
Descrizione fisica | 1 online resource (362 pages) |
Disciplina | 572.56 |
Collana | Techniques in Life Science and Biomedicine for the Non-Expert |
Soggetto topico |
Carbohydrates - Analysis
Glúcids |
Soggetto genere / forma | Llibres electrònics |
ISBN | 3-030-77791-X |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Intro -- Key Features -- Preface -- Acknowledgments -- Disclaimer -- Contents -- About the Author -- Abbreviations -- Chapter 1: The World of Carbohydrates -- 1.1 Carbohydrates Are All Around Us -- 1.2 Carbohydrates in Food -- 1.3 Carbohydrates in Life Science and Biomedicine -- References -- Chapter 2: Basic Knowledge of Glycobiology -- 2.1 Monosaccharides -- 2.1.1 The Molecular Structure -- 2.1.2 d/l Forms of Monosaccharides -- 2.1.3 Specific Monosaccharides -- 2.2 Oligosaccharides -- 2.3 Polysaccharides -- 2.4 Glycoconjugates -- 2.4.1 Glycoproteins -- 2.4.1.1 N-Glycans -- 2.4.1.2 O-Glycans -- 2.4.1.3 Blood Group Determinants -- 2.4.1.4 Different Types of Glycosylation -- 2.4.1.5 Glycosides -- 2.4.2 Proteoglycans and Glycosaminoglycans -- 2.4.3 Glycolipids -- 2.4.4 Glycosylphosphatidyl-Inositol Membrane Anchors -- 2.5 Intrinsic Complexity of Carbohydrates -- 2.6 Working in a Laboratory -- References -- Chapter 3: Carbohydrates Involved in Diseases -- References -- Chapter 4: Detection of Carbohydrates by Colorimetric Methods -- 4.1 Introduction -- 4.2 Preparing Standard Sugar Solutions for Calibration Curves -- 4.3 General Tests -- 4.3.1 Detection of Carbohydrate by a Spot Test -- 4.3.2 Phenol-Sulfuric Acid Assay -- 4.3.3 Anthrone Test -- 4.3.4 Molisch's Test -- 4.3.5 Fehling's Test -- 4.3.6 Benedict's Test -- 4.3.7 Barfoed's Test -- 4.4 Specific Tests -- 4.4.1 Seliwanoff's Test for Ketoses -- 4.4.2 Bial's Test for Pentoses -- 4.4.3 Colorimetric Analysis of (N-Acetyl) Aminosugars -- 4.4.4 Colorimetric Analysis of Uronic Acids -- 4.4.5 Colorimetric Analysis of Sialic Acids -- 4.4.6 Iodine Test for Starch -- References -- Chapter 5: Analytical Techniques to Study Carbohydrates -- 5.1 Structural Parameters -- 5.2 Glycosidic-Bond Cleavage Techniques -- 5.2.1 Sulfuric Acid Hydrolysis -- 5.2.2 Hydrochloric Acid Hydrolysis.
5.2.3 Trifluoroacetic Acid Hydrolysis -- 5.2.4 Methanolysis -- 5.2.5 Formolysis -- 5.2.6 Acetolysis -- 5.2.7 Specific Degradations -- 5.3 Common Separation Techniques for Mono/Oligosaccharides -- 5.3.1 Thin-Layer Chromatography -- 5.3.2 Size-Exclusion Chromatography -- 5.3.3 High-Performance Liquid Chromatography -- 5.3.4 Porous Graphitized Carbon Chromatography -- 5.3.5 Anion/Cation-Exchange Chromatography -- 5.3.6 High-pH Anion-Exchange Chromatography -- 5.3.7 Affinity Chromatography -- 5.4 Detection Techniques for Liquid Chromatography -- 5.4.1 Detectors -- 5.4.2 Labeling of Glycans for Detection -- 5.4.2.1 Labeling by Reductive Amination -- 5.4.2.2 Labeling by Michael Addition with PMP -- 5.4.2.3 Permethylation of Glycans for Mass Spectrometry -- 5.5 Electrophoretic Separation of Carbohydrates -- 5.6 Gas-Liquid Chromatography -- References -- Chapter 6: Monosaccharide Composition Analysis -- 6.1 Introduction -- 6.2 Gas-Chromatographic Monosaccharide Analysis Using TMS Methyl Glycosides -- 6.3 Gas-Chromatographic Monosaccharide Analysis Using Alditol Acetates -- 6.4 Determination of d/l Configuration of Monosaccharides -- 6.5 Monosaccharide Analysis by HPLC Methods -- 6.5.1 High-pH Anion-Exchange Chromatography -- 6.5.2 Ultrahigh-Performance Liquid Chromatography -- 6.6 Determination of Carbohydrate Linkages by Methylation Analysis -- 6.6.1 Chemical Aspects -- 6.6.2 GLC-EI Mass Spectrometry of PMAAs -- 6.6.3 Glycosidic Linkage Determination by HPLC-MS/MS -- References -- Chapter 7: Carbohydrate Analysis of Glycoconjugates -- 7.1 Introduction -- 7.2 Analysis of Glycoproteins -- 7.2.1 A Typical Approach to the Analysis of a Glycoprotein -- 7.2.2 Chemical Release of N- and O-Linked Glycans -- 7.2.3 Enzymatic Release of N-Linked Glycans -- 7.2.3.1 Isolation of N-Glycans and de-N-Glycosylated Protein (Containing O-Glycans). 7.2.4 Release of O-Glycans by Reductive β-Elimination -- 7.3 Analysis of Glycopeptides -- 7.3.1 Preparation of Glycopeptides from Glycoproteins -- 7.3.2 Separation and Isolation of Glycopeptides -- 7.4 Analysis of Proteoglycans and Their Glycosaminoglycans -- 7.5 Analysis of Glycolipids -- 7.5.1 General Aspects -- 7.5.2 Thin-Layer Chromatography of Glycolipids -- 7.5.3 Carbohydrates of Glyco(Sphingo)Lipids -- 7.5.4 Carbohydrates of Glycosylphosphatidylinositol (GPI) Anchors -- 7.6 Analysis of Polysaccharides -- References -- Chapter 8: Structural Characterization of Released Glycans -- 8.1 Introduction -- 8.2 Analysis of N-Glycans -- 8.2.1 Fractionation of N-Glycans -- 8.2.2 Preparation of Glucose Oligomers Standard (Dextran Ladder) -- 8.2.3 Enzymatic Sequence Analysis of N-Glycans -- 8.3 Analysis of O-Glycans -- 8.3.1 General Aspects -- 8.3.2 Mucin Glycoproteins -- 8.3.3 Fractionation of O-Glycans -- References -- Chapter 9: Analysis of Sialic Acids -- 9.1 General Aspects -- 9.2 Characterization of Sialic Acid Residues -- 9.2.1 Colorimetric Determination -- 9.2.2 Gas-Liquid Chromatographic Determination -- 9.2.3 Release of Sialic Acids by Mild Acid Hydrolysis -- 9.2.4 Analysis of DMB-Labeled Sialic Acid -- 9.2.5 Release of Sialic Acids by Enzymatic Digestion -- 9.3 Mass Spectrometry of Sialic Acids -- 9.4 1H NMR Spectroscopy of Sialic Acids -- References -- Chapter 10: Carbohydrate Microarray Technology -- References -- Chapter 11: Analysis of Carbohydrates by Mass Spectrometry -- 11.1 General Aspects -- 11.2 Electron Impact Mass Spectrometry (EI-MS) -- 11.3 Mass Spectrometry of Glycans -- 11.3.1 General -- 11.3.2 Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS) -- 11.3.3 Electrospray Ionization-Mass Spectrometry (ESI-MS) -- 11.3.4 Tandem Mass Spectrometry -- 11.3.5 Mass Fragmentation of Oligosaccharides. References -- Chapter 12: Analysis of Carbohydrates by Nuclear Magnetic Resonance Spectroscopy -- 12.1 Introduction -- 12.2 Principle of NMR Spectroscopy -- 12.3 NMR Spectroscopy for Carbohydrates -- 12.3.1 Sample Preparation and Measuring -- 12.3.2 1H NMR Structural-Reporter-Group Concept -- 12.3.3 13C NMR Spectroscopy -- 12.4 NMR Spectroscopy for Glycans -- 12.4.1 Two-Dimensional NMR Spectroscopy -- 12.4.2 Spectral Interpretation and Assignments -- 12.5 NMR Spectroscopy of Polysaccharides -- 12.5.1 Summarized NMR Measuring Procedure -- References -- Chapter 13: Glycobioinformatics -- References -- Chapter 14: Concluding Remarks -- References -- Appendix A -- Appendix B -- Appendix C -- Glossary -- Index. |
Record Nr. | UNINA-9910506390003321 |
Gerwig Gerrit J. | ||
Cham, Switzerland : , : Springer, , [2021] | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Bioinformatics for glycobiology and glycomics [[electronic resource] ] : an introduction / / edited by Claus-Wilhelm von der Lieth, Thomas Lütteke, and Martin Frank |
Pubbl/distr/stampa | Chichester ; ; Hoboken, NJ, : Wiley-Blackwell, c2009 |
Descrizione fisica | 1 online resource (505 p.) |
Disciplina |
572.56
572.80285 |
Altri autori (Persone) |
LiethClaus-Wilhelm von der
LüttekeThomas FrankMartin <1963-> |
Soggetto topico |
Glycomics
Glycoconjugates - Research - Data processing Bioinformatics |
ISBN |
1-282-31317-7
9786612313172 0-470-02961-7 0-470-02960-9 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Bioinformatics for Glycobiologyand Glycomics; Contents; List of Contributors; Preface; Section 1: Introduction; 1. Glycobiology, Glycomics and (Bio)Informatics; Section 2: Carbohydrate Structures; 2. Introduction to Carbohydrate Structure and Diversity; 3. Digital Representations of Oligo- and Polysaccharides; 4. Evolutionary Considerations in Studying the Sialome: Sialic; Section 3: Carbohydrate-active Enzymes and Glycosylation; 5. Carbohydrate-active Enzymes Database: Principles and Classification of Glycosyltransferases; 6. Other Databases Providing Glycoenzyme Data
7. Bioinformatics Analysis of Glycan Structures from a Genomic Perspective8. Glycosylation of Proteins; 9. Prediction of Glycosylation Sites in Proteins; Section 4: Experimental Methods - Bioinformatic Requirements; 10. Experimental Methods for the Analysis of Glycans and Their Bioinformatics Requirements; 11. Analysis of N- and O-Glycans of Glycoproteins by HPLC Technology; 12. Glycomic Mass Spectrometric Analysis and Data Interpretation Tools; 13. Software Tools for Semi-automatic Interpretation of Mass Spectra of Glycans 14. Informatics Concepts to Decode Structure-Function Relationships of Glycosaminoglycans15. NMR Databases and Tools for Automatic Interpretation of Spectra of Carbohydrates; 16. Automatic Spectrum Interpretation Based on Increment Rules: CASPER; 17. Interpretation of 13C NMR Spectra by Artificial Neural Network Techniques (NeuroCarb); Section 5: 3D Structures of Complex Carbohydrates; 18. Conformational Analysis of Carbohydrates - A Historical Overview; 19. Predicting Carbohydrate 3D Structures Using Theoretical Methods 20. Synergy of Computational and Experimental Methods in Carbohydrate 3D Structure Determination and ValidationSection 6: Protein-Carbohydrate Interaction; 21. Structural Features of Lectins and Their Binding Sites; 22. Statistical Analysis of Protein-Carbohydrate Complexes Contained in the PDB; Section 7: Appendices; Appendix 1: List of Available Websites; Appendix 2: Glossary; Index; Color Plates; Cover Page |
Record Nr. | UNINA-9910139871403321 |
Chichester ; ; Hoboken, NJ, : Wiley-Blackwell, c2009 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Bioinformatics for glycobiology and glycomics [[electronic resource] ] : an introduction / / edited by Claus-Wilhelm von der Lieth, Thomas Lütteke, and Martin Frank |
Pubbl/distr/stampa | Chichester ; ; Hoboken, NJ, : Wiley-Blackwell, c2009 |
Descrizione fisica | 1 online resource (505 p.) |
Disciplina |
572.56
572.80285 |
Altri autori (Persone) |
LiethClaus-Wilhelm von der
LüttekeThomas FrankMartin <1963-> |
Soggetto topico |
Glycomics
Glycoconjugates - Research - Data processing Bioinformatics |
ISBN |
1-282-31317-7
9786612313172 0-470-02961-7 0-470-02960-9 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Bioinformatics for Glycobiologyand Glycomics; Contents; List of Contributors; Preface; Section 1: Introduction; 1. Glycobiology, Glycomics and (Bio)Informatics; Section 2: Carbohydrate Structures; 2. Introduction to Carbohydrate Structure and Diversity; 3. Digital Representations of Oligo- and Polysaccharides; 4. Evolutionary Considerations in Studying the Sialome: Sialic; Section 3: Carbohydrate-active Enzymes and Glycosylation; 5. Carbohydrate-active Enzymes Database: Principles and Classification of Glycosyltransferases; 6. Other Databases Providing Glycoenzyme Data
7. Bioinformatics Analysis of Glycan Structures from a Genomic Perspective8. Glycosylation of Proteins; 9. Prediction of Glycosylation Sites in Proteins; Section 4: Experimental Methods - Bioinformatic Requirements; 10. Experimental Methods for the Analysis of Glycans and Their Bioinformatics Requirements; 11. Analysis of N- and O-Glycans of Glycoproteins by HPLC Technology; 12. Glycomic Mass Spectrometric Analysis and Data Interpretation Tools; 13. Software Tools for Semi-automatic Interpretation of Mass Spectra of Glycans 14. Informatics Concepts to Decode Structure-Function Relationships of Glycosaminoglycans15. NMR Databases and Tools for Automatic Interpretation of Spectra of Carbohydrates; 16. Automatic Spectrum Interpretation Based on Increment Rules: CASPER; 17. Interpretation of 13C NMR Spectra by Artificial Neural Network Techniques (NeuroCarb); Section 5: 3D Structures of Complex Carbohydrates; 18. Conformational Analysis of Carbohydrates - A Historical Overview; 19. Predicting Carbohydrate 3D Structures Using Theoretical Methods 20. Synergy of Computational and Experimental Methods in Carbohydrate 3D Structure Determination and ValidationSection 6: Protein-Carbohydrate Interaction; 21. Structural Features of Lectins and Their Binding Sites; 22. Statistical Analysis of Protein-Carbohydrate Complexes Contained in the PDB; Section 7: Appendices; Appendix 1: List of Available Websites; Appendix 2: Glossary; Index; Color Plates; Cover Page |
Record Nr. | UNINA-9910830644903321 |
Chichester ; ; Hoboken, NJ, : Wiley-Blackwell, c2009 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Biosynthesis and biodegradation of cellulose / edited by Candace H. Haigler, Paul J. Weimer |
Pubbl/distr/stampa | New York : M. Dekker, c1991 |
Descrizione fisica | xi, 694 p. : ill. ; 24 cm |
Disciplina | 572.56 |
Altri autori (Persone) |
Haigler, Candace H.
Weimer, Paul J. |
Soggetto topico |
Cellulose - Biodegradation
Cellulose - Synthesis Microbial metabolism Cellulose Biodegradation Cellulose - biosynthesis Microbiology |
ISBN | 0824783875 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNISALENTO-991000746889707536 |
New York : M. Dekker, c1991 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. del Salento | ||
|
Boronic acids in saccharide recognition [[electronic resource] /] / Tony D. James, Marcus D. Phillips, Seiji Shinkai |
Autore | James Tony D |
Pubbl/distr/stampa | Cambridge, : RSC Pub., c2006 |
Descrizione fisica | 1 online resource (185 p.) |
Disciplina | 572.56 |
Altri autori (Persone) |
PhillipsMarcus D
ShinkaiSeiji |
Collana | Monographs in supramolecular chemistry |
Soggetto topico | Saccharides |
Soggetto genere / forma | Electronic books. |
ISBN |
1-84755-761-9
1-61583-351-X |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | ""Boronic Acids_UPDATED OFC COVER""; ""i_x""; ""001_167""; ""168_172""; ""173_174"" |
Record Nr. | UNINA-9910455962203321 |
James Tony D | ||
Cambridge, : RSC Pub., c2006 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Boronic acids in saccharide recognition [[electronic resource] /] / Tony D. James, Marcus D. Phillips, Seiji Shinkai |
Autore | James Tony D |
Pubbl/distr/stampa | Cambridge, : RSC Pub., c2006 |
Descrizione fisica | 1 online resource (185 p.) |
Disciplina | 572.56 |
Altri autori (Persone) |
PhillipsMarcus D
ShinkaiSeiji |
Collana | Monographs in supramolecular chemistry |
Soggetto topico | Saccharides |
ISBN |
1-84755-761-9
1-61583-351-X |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | ""Boronic Acids_UPDATED OFC COVER""; ""i_x""; ""001_167""; ""168_172""; ""173_174"" |
Record Nr. | UNINA-9910780735203321 |
James Tony D | ||
Cambridge, : RSC Pub., c2006 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Boronic acids in saccharide recognition / / Tony D. James, Marcus D. Phillips, Seiji Shinkai |
Autore | James Tony D |
Edizione | [2nd ed.] |
Pubbl/distr/stampa | Cambridge, : RSC Pub., c2006 |
Descrizione fisica | 1 online resource (185 p.) |
Disciplina | 572.56 |
Altri autori (Persone) |
PhillipsMarcus D
ShinkaiSeiji |
Collana |
ISSN
Monographs in supramolecular chemistry |
Soggetto topico | Saccharides |
ISBN |
1-84755-761-9
1-61583-351-X |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | ""Boronic Acids_UPDATED OFC COVER""; ""i_x""; ""001_167""; ""168_172""; ""173_174"" |
Record Nr. | UNINA-9910814524003321 |
James Tony D | ||
Cambridge, : RSC Pub., c2006 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Carbohydrate analysis : a practical approach / edited by M. F. Chaplin, J. F. Kennedy |
Pubbl/distr/stampa | Oxford ; Washington : IRL Press, c1986 |
Descrizione fisica | xv, 228 p. : ill. ; 23 cm |
Disciplina | 572.56 |
Altri autori (Persone) |
Chaplin, Martin F.
Kennedy, John F. |
Collana | The Practical approach series |
Soggetto topico | Carbohydrates - Analysis |
ISBN |
0947946446
0947946683 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNISALENTO-991000465449707536 |
Oxford ; Washington : IRL Press, c1986 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. del Salento | ||
|
Carbohydrate bioengineering [[electronic resource] ] : interdisciplinary approaches / / edited by Tuula T. Teeri ... [et al.] |
Pubbl/distr/stampa | Cambridge, U.K., : Royal Society of Chemistry, c2002 |
Descrizione fisica | 1 online resource (208 p.) |
Disciplina | 572.56 |
Altri autori (Persone) | TeeriTuula T |
Collana | Special publication |
Soggetto topico | Carbohydrates - Biotechnology |
Soggetto genere / forma | Electronic books. |
ISBN | 1-84755-032-0 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
BK9780854048267-FX001; 9780854048267txt; BK9780854048267-FP001; BK9780854048267-FP005; BK9780854048267-FP007; BK9780854048267-00001; BK9780854048267-00003; BK9780854048267-00009; BK9780854048267-00011; BK9780854048267-00019; BK9780854048267-00028; BK9780854048267-00034; BK9780854048267-00042; BK9780854048267-00049; BK9780854048267-00057; BK9780854048267-00065; BK9780854048267-00067; BK9780854048267-00076; BK9780854048267-00082; BK9780854048267-00087; BK9780854048267-00089; BK9780854048267-00099; BK9780854048267-00106; BK9780854048267-00113; BK9780854048267-00125; BK9780854048267-00127
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Record Nr. | UNINA-9910455255903321 |
Cambridge, U.K., : Royal Society of Chemistry, c2002 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Carbohydrate bioengineering [[electronic resource] ] : interdisciplinary approaches / / edited by Tuula T. Teeri ... [et al.] |
Pubbl/distr/stampa | Cambridge, U.K., : Royal Society of Chemistry, c2002 |
Descrizione fisica | 1 online resource (208 p.) |
Disciplina | 572.56 |
Altri autori (Persone) | TeeriTuula T |
Collana | Special publication |
Soggetto topico | Carbohydrates - Biotechnology |
ISBN | 1-84755-032-0 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
BK9780854048267-FX001; 9780854048267txt; BK9780854048267-FP001; BK9780854048267-FP005; BK9780854048267-FP007; BK9780854048267-00001; BK9780854048267-00003; BK9780854048267-00009; BK9780854048267-00011; BK9780854048267-00019; BK9780854048267-00028; BK9780854048267-00034; BK9780854048267-00042; BK9780854048267-00049; BK9780854048267-00057; BK9780854048267-00065; BK9780854048267-00067; BK9780854048267-00076; BK9780854048267-00082; BK9780854048267-00087; BK9780854048267-00089; BK9780854048267-00099; BK9780854048267-00106; BK9780854048267-00113; BK9780854048267-00125; BK9780854048267-00127
BK9780854048267-00135BK9780854048267-00143; BK9780854048267-00151; BK9780854048267-00153; BK9780854048267-00160; BK9780854048267-00169; BK9780854048267-00171; BK9780854048267-00178; BK9780854048267-00186; BK9780854048267-00194 |
Record Nr. | UNINA-9910778317503321 |
Cambridge, U.K., : Royal Society of Chemistry, c2002 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|