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Biochemical methods : a practical approach / / Anusha Bhaskar
| Biochemical methods : a practical approach / / Anusha Bhaskar |
| Autore | Bhaskar Anusha |
| Pubbl/distr/stampa | Oxford, England : , : Alpha Science International Ltd., , 2014 |
| Descrizione fisica | 1 online resource (174 pages) : illustrations, tables |
| Disciplina | 572.078 |
| Soggetto topico | Biochemistry |
| Soggetto genere / forma | Electronic books. |
| ISBN | 1-78332-033-8 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910467300103321 |
Bhaskar Anusha
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| Oxford, England : , : Alpha Science International Ltd., , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Biochemical methods : a practical approach / / Anusha Bhaskar
| Biochemical methods : a practical approach / / Anusha Bhaskar |
| Autore | Bhaskar Anusha |
| Pubbl/distr/stampa | Oxford, England : , : Alpha Science International Ltd., , 2014 |
| Descrizione fisica | 1 online resource (174 pages) : illustrations, tables |
| Disciplina | 572.078 |
| Soggetto topico | Biochemistry |
| ISBN | 1-78332-033-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910794885403321 |
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Bhaskar Anusha
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||
| Oxford, England : , : Alpha Science International Ltd., , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biochemical methods : a practical approach / / Anusha Bhaskar
| Biochemical methods : a practical approach / / Anusha Bhaskar |
| Autore | Bhaskar Anusha |
| Pubbl/distr/stampa | Oxford, England : , : Alpha Science International Ltd., , 2014 |
| Descrizione fisica | 1 online resource (174 pages) : illustrations, tables |
| Disciplina | 572.078 |
| Soggetto topico | Biochemistry |
| ISBN | 1-78332-033-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910810147203321 |
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Bhaskar Anusha
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||
| Oxford, England : , : Alpha Science International Ltd., , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka
| Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka |
| Autore | Gerczei Timea |
| Pubbl/distr/stampa | Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 |
| Descrizione fisica | 1 online resource (186 pages) : illustrations, (some colour) |
| Disciplina | 572.078 |
| Soggetto topico |
Biochemistry
Molecular biology Drug resistance in microorganisms |
| ISBN |
1-5231-0070-2
3-11-042625-0 3-11-041133-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Preface -- Contents -- 1 Introducing the Bacterial Antibiotic Sensor Mini Project -- 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- 3 Designing Primers for Site-Directed Mutagenesis -- 4 Performing Site-Directed Mutagenesis -- 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- 11 Understanding the Importance of Buffers in Biological Systems -- 12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- List of Figures |
| Record Nr. | UNINA-9910132278903321 |
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Gerczei Timea
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||
| Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka
| Biochemistry laboratory manual for undergraduates : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka |
| Autore | Gerczei Timea |
| Pubbl/distr/stampa | Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 |
| Descrizione fisica | 1 online resource (186 pages) : illustrations, (some colour) |
| Disciplina | 572.078 |
| Soggetto topico |
Biochemistry
Molecular biology Drug resistance in microorganisms |
| ISBN |
1-5231-0070-2
3-11-042625-0 3-11-041133-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Preface -- Contents -- 1 Introducing the Bacterial Antibiotic Sensor Mini Project -- 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- 3 Designing Primers for Site-Directed Mutagenesis -- 4 Performing Site-Directed Mutagenesis -- 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- 11 Understanding the Importance of Buffers in Biological Systems -- 12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- List of Figures |
| Record Nr. | UNISA-996308751403316 |
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Gerczei Timea
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| Warsaw, [Poland] ; ; Berlin, [Germany] : , : De Gruyter Open, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
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College biochemistry . II [[electronic resource] /] / L. Indira, K. Nagaraju, Zameer Ahmed K
| College biochemistry . II [[electronic resource] /] / L. Indira, K. Nagaraju, Zameer Ahmed K |
| Autore | Indira L |
| Edizione | [Rev. ed.] |
| Pubbl/distr/stampa | Mumbai [India], : Himalaya Pub. House, 2010 |
| Descrizione fisica | 1 online resource (251 p.) |
| Disciplina | 572.078 |
| Altri autori (Persone) |
NagarajuK
KZameer Ahmed |
| Collana | College biochemistry |
| Soggetto topico | Biochemistry |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-282-81275-0
9786612812750 1-4416-7542-6 93-5043-140-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | COVER; Contents; Introduction; Hydrocarbons; Cycloalkanes; Arenes; Organic Halides and Organometallic Compounds; Alcochols; Phenols; Carbonyl Compounds; Gases; Solids; Chemical Equilibrium; Chemical Kinetics; Thermodynamics:Introduction and Terminology; Phase Rule; LABORATORY MANUAL |
| Record Nr. | UNINA-9910459940503321 |
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Indira L
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| Mumbai [India], : Himalaya Pub. House, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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College biochemistry . II [[electronic resource] /] / L. Indira, K. Nagaraju, Zameer Ahmed K
| College biochemistry . II [[electronic resource] /] / L. Indira, K. Nagaraju, Zameer Ahmed K |
| Autore | Indira L |
| Edizione | [Rev. ed.] |
| Pubbl/distr/stampa | Mumbai [India], : Himalaya Pub. House, 2010 |
| Descrizione fisica | 1 online resource (251 p.) |
| Disciplina | 572.078 |
| Altri autori (Persone) |
NagarajuK
KZameer Ahmed |
| Collana | College biochemistry |
| Soggetto topico | Biochemistry |
| ISBN |
1-282-81275-0
9786612812750 1-4416-7542-6 93-5043-140-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | COVER; Contents; Introduction; Hydrocarbons; Cycloalkanes; Arenes; Organic Halides and Organometallic Compounds; Alcochols; Phenols; Carbonyl Compounds; Gases; Solids; Chemical Equilibrium; Chemical Kinetics; Thermodynamics:Introduction and Terminology; Phase Rule; LABORATORY MANUAL |
| Record Nr. | UNINA-9910785658203321 |
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Indira L
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| Mumbai [India], : Himalaya Pub. House, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
A manual for biochemistry protocols [[electronic resource] /] / Markus R. Wenk, Aaron Zefrin Fernandis
| A manual for biochemistry protocols [[electronic resource] /] / Markus R. Wenk, Aaron Zefrin Fernandis |
| Autore | Wenk Markus R |
| Pubbl/distr/stampa | Singapore ; ; Hackensack, NJ, : World Scientific, c2007 |
| Descrizione fisica | 1 online resource |
| Disciplina |
572
572.078 |
| Altri autori (Persone) | FernandisAaron Zefrin |
| Collana | Manuals in biomedical research |
| Soggetto topico | Biochemistry |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-12115-0
9786611121150 981-270-831-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910450709003321 |
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Wenk Markus R
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| Singapore ; ; Hackensack, NJ, : World Scientific, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
A manual for biochemistry protocols [[electronic resource] /] / Markus R. Wenk, Aaron Zefrin Fernandis
| A manual for biochemistry protocols [[electronic resource] /] / Markus R. Wenk, Aaron Zefrin Fernandis |
| Autore | Wenk Markus R |
| Pubbl/distr/stampa | Singapore ; ; Hackensack, NJ, : World Scientific, c2007 |
| Descrizione fisica | 1 online resource |
| Disciplina |
572
572.078 |
| Altri autori (Persone) | FernandisAaron Zefrin |
| Collana | Manuals in biomedical research |
| Soggetto topico | Biochemistry |
| ISBN |
1-281-12115-0
9786611121150 981-270-831-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910784039003321 |
|
Wenk Markus R
|
||
| Singapore ; ; Hackensack, NJ, : World Scientific, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
A manual for biochemistry protocols / / Markus R. Wenk, Aaron Zefrin Fernandis
| A manual for biochemistry protocols / / Markus R. Wenk, Aaron Zefrin Fernandis |
| Autore | Wenk Markus R |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Singapore ; ; Hackensack, NJ, : World Scientific, c2007 |
| Descrizione fisica | 1 online resource |
| Disciplina |
572
572.078 |
| Altri autori (Persone) | FernandisAaron Zefrin |
| Collana | Manuals in biomedical research |
| Soggetto topico | Biochemistry |
| ISBN |
9786611121150
9781281121158 1281121150 9789812708311 9812708316 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Intro -- Contents -- Preface -- List of Figures -- List of Contributors -- A Protein Purification -- Summary -- A.1 Protein Precipitation -- A.2 Column Chromatography -- A.2.1 Ionic Exchange Chromatography -- A.2.2 Size-Exclusion Chromatography -- A.2.3 Affinity Chromatography -- A.2.4 Purification of Recombinant Proteins -- A.2.5 Commercially Prepacked Column Kits -- Notes -- B Protein Analysis -- B.1 Protein Estimation -- B.1.1 Absorbance Assays -- B.1.2 Colourimetric Assays -- B.1.3 Commercial Protein Estimation Kits -- B.2 Spectrometric Analysis -- B.3 SDS-PAGE -- B.4 Gel Staining -- B.4.1 Coomassie Blue Staining -- B.4.2 Silver Staining -- B.5 Western Blotting -- B.6 Immunoprecipitations -- Notes -- C Lipid Analysis -- C.1 Lipid Extraction -- C.1.1 Modified Bligh and Dyers Method for Phospholipid Extraction -- C.1.2 Folch Extraction -- C.1.3 Grey Method for Phosphatidylinositol Phosphate Extraction -- C.1.4 Modified Alex Brown Method for Phosphatidylinositol Phosphate Extraction -- C.1.5 Hexane Extraction for Neutral Lipids -- C.1.6 Glycolipid Extraction -- C.2 Thin Layer Chromatography -- Notes -- D Mammalian Cell Culture -- D.1 Primary Culture -- D.1.1 Primary Cell Isolation and Culturing -- D.1.2 Splenocyte Isolation -- D.1.3 Isolation of Peripheral Blood Lymphocytes -- D.2 Tissue Culture -- D.2.1 Adherent Cells -- D.2.2 Suspension Cells -- D.2.3 Cell Maintenance -- D.3 Cell Count -- D.4 Mammalian Transfection -- D.4.1 Calcium Phosphate Coprecipitation -- D.4.2 Electroporation Protocol -- D.4.3 Lipofectamine Transfection Protocol -- Notes -- E Microscopy -- E.1 Poly-L-Lysine Immobilisation -- E.2 Immunofluorescence and Confocal Microscopy -- E.3 Immunohistochemistry -- Notes -- F Assays -- F.1 Enzyme Assays -- F.1.1 Enzyme Kinetics: Basics -- F.1.2 Lipid Kinase Assay -- F.1.3 Protein Kinase Assay.
F.1.4 Protein Tyrosine Phosphatase Assay -- F.1.5 Alkaline Phosphatase Assay -- F.1.6 Caspase Assay -- F.2 Functional Assays -- F.2.1 Apoptosis Assay -- F.2.2 XTT Cell Proliferation Assay [(2,3-Bis (2-methoxy-4-nitro-5-sulfophenyl)- 2H-tetrazolium-5-carboxanilide)] -- F.2.3 Chemotaxis Assay -- F.2.4 Matrigel Invasion or Chemoinvasion Assay -- Notes -- G Autoradiography -- Summary -- Notes -- Suggested Readings -- Abbreviations -- Appendices -- Appendix 1 Safety Issues -- Appendix 2 Buffer Chart -- Appendix 3 Composition of Regular Buffers or Stock Solutions -- Index. |
| Record Nr. | UNINA-9910961783603321 |
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Wenk Markus R
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| Singapore ; ; Hackensack, NJ, : World Scientific, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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