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Bioelectrochemistry [[electronic resource] ] : fundamentals, experimental techniques and applications / / edited by P.N. Bartlett
Bioelectrochemistry [[electronic resource] ] : fundamentals, experimental techniques and applications / / edited by P.N. Bartlett
Pubbl/distr/stampa Chichester, England ; ; Hoboken, NJ, : John Wiley & Sons, c2008
Descrizione fisica 1 online resource (496 p.)
Disciplina 572.437
572/.437
Altri autori (Persone) BartlettPhilip N <1956-> (Philip Nigel)
Soggetto topico Bioelectrochemistry
Soggetto genere / forma Electronic books.
ISBN 1-282-34318-1
9786612343186
0-470-75384-6
0-470-75383-8
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Bioelectrochemistry Fundamentals, Experimental Techniques and Applications; Contents; List of Contributors; Preface; 1 Bioenergetics and Biological Electron Transport; 1.1 Introduction; 1.2 Biological Cells; 1.3 Chemiosmosis; 1.3.1 The Proton Motive Force; 1.3.2 The Synthesis of ATP; 1.4 Electron Transport Chains; 1.4.1 The Mitochondrion; 1.4.2 The NADH-CoQ Reductase Complex; 1.4.3 The Succinate-CoQ Reductase Complex; 1.4.4 The CoQH 2 -Cytc Reductase Complex; 1.4.5 The Cytc Oxidase Complex; 1.4.6 Electron Transport Chains in Bacteria; 1.4.7 Electron Transfer in Photosynthesis
1.4.8 Photosystem II1.4.9 Cytochromebf Complex; 1.4.10 Photosystem I; 1.4.11 Bacterial Photosynthesis; 1.5 Redox Components; 1.5.1 Quinones; 1.5.2 Flavins; 1.5.3 NAD(P)H; 1.5.4 Hemes; 1.5.5 Iron-Sulfur Clusters; 1.5.6 Copper Centres; 1.6 Governing Principles; 1.6.1 Spatial Separation; 1.6.2 Energetics: Redox Potentials; 1.6.3 Kinetics: Electron Transfer Rate Constants; 1.6.4 Size of Proteins; 1.6.5 One-Electron and Two-Electron Couples; 1.7 ATP Synthase; 1.8 Conclusion; References; 2 Electrochemistry of Redox Enzymes; 2.1 Introduction; 2.1.1 Historical Perspective
2.1.2 Examples of Soluble Mediators2.1.3 Development of Protein-Film Voltammetry and Direct Enzyme Electrochemistry; 2.2 Mediated Enzyme Electrochemistry; 2.2.1 Electron Mediation; 2.2.2 Wiring with Redox Metallopolymer Hydrogels; 2.2.3 Wiring with Conducting Polymers; 2.2.4 NAD(P) /NAD(P)H Dependent Enzymes; 2.2.5 Regeneration of NAD(P)H from NAD(P); 2.2.6 Regeneration of NAD(P) from NAD(P)H; 2.3 Direct Electron Transfer between Electrodes and Enzymes; 2.3.1 Enzymes in Solution; 2.3.2 Enzyme-Film Voltammetry: Basic Theory; 2.3.3 Adsorbed and Coadsorbed Enzyme Monolayers
2.3.4 Self-Assembled Monolayers and Covalently Attached Enzymes2.3.5 Enzymes on Carbon Nanotube Electrodes; 2.3.6 Enzymes in Lipid Bilayer Films; 2.3.7 Polyion Films and Layer-by-Layer Methods; 2.4 Outlook for the Future; Acknowledgements; References; 3 Biological Membranes and Membrane Mimics; 3.1 Introduction; 3.2 Membrane Structure and Composition; 3.2.1 Membrane Structure; 3.2.2 Membrane Lipids; 3.2.3 Membrane Proteins; 3.3 Models of Membrane Structure; 3.3.1 Lipid Monolayers; 3.3.2 Bilayer Lipid Membranes (BLM); 3.3.3 Supported Bilayer Lipid Membranes; 3.3.4 Liposomes
3.4 Ordering, Conformation and Molecular Dynamics of Lipid Bilayers3.4.1 Structural Parameters of Lipid Bilayers Measured by X-ray Diffraction; 3.4.2 Interactions between Bilayers; 3.4.3 Dynamics and Order Parameters of Bilayers Determined by EPR and NMR Spectroscopy and by Optical Spectroscopy Methods; 3.5 Phase Transitions of Lipid Bilayers; 3.5.1 Lyotropic and Thermotropic Transitions; 3.5.2 Thermodynamics of Phase Transitions; 3.5.3 Trans-Gauche Isomerization; 3.5.4 Order Parameter; 3.5.5 Cooperativity of Transition; 3.5.6 Theory of Phase Transitions
3.6 Mechanical Properties of Lipid Bilayers
Record Nr. UNINA-9910146268503321
Chichester, England ; ; Hoboken, NJ, : John Wiley & Sons, c2008
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Bioelectrochemistry [[electronic resource] ] : fundamentals, experimental techniques and applications / / edited by P.N. Bartlett
Bioelectrochemistry [[electronic resource] ] : fundamentals, experimental techniques and applications / / edited by P.N. Bartlett
Pubbl/distr/stampa Chichester, England ; ; Hoboken, NJ, : John Wiley & Sons, c2008
Descrizione fisica 1 online resource (496 p.)
Disciplina 572.437
572/.437
Altri autori (Persone) BartlettPhilip N <1956-> (Philip Nigel)
Soggetto topico Bioelectrochemistry
ISBN 1-282-34318-1
9786612343186
0-470-75384-6
0-470-75383-8
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Bioelectrochemistry Fundamentals, Experimental Techniques and Applications; Contents; List of Contributors; Preface; 1 Bioenergetics and Biological Electron Transport; 1.1 Introduction; 1.2 Biological Cells; 1.3 Chemiosmosis; 1.3.1 The Proton Motive Force; 1.3.2 The Synthesis of ATP; 1.4 Electron Transport Chains; 1.4.1 The Mitochondrion; 1.4.2 The NADH-CoQ Reductase Complex; 1.4.3 The Succinate-CoQ Reductase Complex; 1.4.4 The CoQH 2 -Cytc Reductase Complex; 1.4.5 The Cytc Oxidase Complex; 1.4.6 Electron Transport Chains in Bacteria; 1.4.7 Electron Transfer in Photosynthesis
1.4.8 Photosystem II1.4.9 Cytochromebf Complex; 1.4.10 Photosystem I; 1.4.11 Bacterial Photosynthesis; 1.5 Redox Components; 1.5.1 Quinones; 1.5.2 Flavins; 1.5.3 NAD(P)H; 1.5.4 Hemes; 1.5.5 Iron-Sulfur Clusters; 1.5.6 Copper Centres; 1.6 Governing Principles; 1.6.1 Spatial Separation; 1.6.2 Energetics: Redox Potentials; 1.6.3 Kinetics: Electron Transfer Rate Constants; 1.6.4 Size of Proteins; 1.6.5 One-Electron and Two-Electron Couples; 1.7 ATP Synthase; 1.8 Conclusion; References; 2 Electrochemistry of Redox Enzymes; 2.1 Introduction; 2.1.1 Historical Perspective
2.1.2 Examples of Soluble Mediators2.1.3 Development of Protein-Film Voltammetry and Direct Enzyme Electrochemistry; 2.2 Mediated Enzyme Electrochemistry; 2.2.1 Electron Mediation; 2.2.2 Wiring with Redox Metallopolymer Hydrogels; 2.2.3 Wiring with Conducting Polymers; 2.2.4 NAD(P) /NAD(P)H Dependent Enzymes; 2.2.5 Regeneration of NAD(P)H from NAD(P); 2.2.6 Regeneration of NAD(P) from NAD(P)H; 2.3 Direct Electron Transfer between Electrodes and Enzymes; 2.3.1 Enzymes in Solution; 2.3.2 Enzyme-Film Voltammetry: Basic Theory; 2.3.3 Adsorbed and Coadsorbed Enzyme Monolayers
2.3.4 Self-Assembled Monolayers and Covalently Attached Enzymes2.3.5 Enzymes on Carbon Nanotube Electrodes; 2.3.6 Enzymes in Lipid Bilayer Films; 2.3.7 Polyion Films and Layer-by-Layer Methods; 2.4 Outlook for the Future; Acknowledgements; References; 3 Biological Membranes and Membrane Mimics; 3.1 Introduction; 3.2 Membrane Structure and Composition; 3.2.1 Membrane Structure; 3.2.2 Membrane Lipids; 3.2.3 Membrane Proteins; 3.3 Models of Membrane Structure; 3.3.1 Lipid Monolayers; 3.3.2 Bilayer Lipid Membranes (BLM); 3.3.3 Supported Bilayer Lipid Membranes; 3.3.4 Liposomes
3.4 Ordering, Conformation and Molecular Dynamics of Lipid Bilayers3.4.1 Structural Parameters of Lipid Bilayers Measured by X-ray Diffraction; 3.4.2 Interactions between Bilayers; 3.4.3 Dynamics and Order Parameters of Bilayers Determined by EPR and NMR Spectroscopy and by Optical Spectroscopy Methods; 3.5 Phase Transitions of Lipid Bilayers; 3.5.1 Lyotropic and Thermotropic Transitions; 3.5.2 Thermodynamics of Phase Transitions; 3.5.3 Trans-Gauche Isomerization; 3.5.4 Order Parameter; 3.5.5 Cooperativity of Transition; 3.5.6 Theory of Phase Transitions
3.6 Mechanical Properties of Lipid Bilayers
Record Nr. UNINA-9910830066403321
Chichester, England ; ; Hoboken, NJ, : John Wiley & Sons, c2008
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Bioelectrochemistry : fundamentals, experimental techniques and applications / / edited by P.N. Bartlett
Bioelectrochemistry : fundamentals, experimental techniques and applications / / edited by P.N. Bartlett
Pubbl/distr/stampa Chichester, England ; ; Hoboken, NJ, : John Wiley & Sons, c2008
Descrizione fisica 1 online resource (496 p.)
Disciplina 572/.437
Altri autori (Persone) BartlettPhilip N <1956-> (Philip Nigel)
Soggetto topico Bioelectrochemistry
ISBN 1-282-34318-1
9786612343186
0-470-75384-6
0-470-75383-8
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Bioelectrochemistry Fundamentals, Experimental Techniques and Applications; Contents; List of Contributors; Preface; 1 Bioenergetics and Biological Electron Transport; 1.1 Introduction; 1.2 Biological Cells; 1.3 Chemiosmosis; 1.3.1 The Proton Motive Force; 1.3.2 The Synthesis of ATP; 1.4 Electron Transport Chains; 1.4.1 The Mitochondrion; 1.4.2 The NADH-CoQ Reductase Complex; 1.4.3 The Succinate-CoQ Reductase Complex; 1.4.4 The CoQH 2 -Cytc Reductase Complex; 1.4.5 The Cytc Oxidase Complex; 1.4.6 Electron Transport Chains in Bacteria; 1.4.7 Electron Transfer in Photosynthesis
1.4.8 Photosystem II1.4.9 Cytochromebf Complex; 1.4.10 Photosystem I; 1.4.11 Bacterial Photosynthesis; 1.5 Redox Components; 1.5.1 Quinones; 1.5.2 Flavins; 1.5.3 NAD(P)H; 1.5.4 Hemes; 1.5.5 Iron-Sulfur Clusters; 1.5.6 Copper Centres; 1.6 Governing Principles; 1.6.1 Spatial Separation; 1.6.2 Energetics: Redox Potentials; 1.6.3 Kinetics: Electron Transfer Rate Constants; 1.6.4 Size of Proteins; 1.6.5 One-Electron and Two-Electron Couples; 1.7 ATP Synthase; 1.8 Conclusion; References; 2 Electrochemistry of Redox Enzymes; 2.1 Introduction; 2.1.1 Historical Perspective
2.1.2 Examples of Soluble Mediators2.1.3 Development of Protein-Film Voltammetry and Direct Enzyme Electrochemistry; 2.2 Mediated Enzyme Electrochemistry; 2.2.1 Electron Mediation; 2.2.2 Wiring with Redox Metallopolymer Hydrogels; 2.2.3 Wiring with Conducting Polymers; 2.2.4 NAD(P) /NAD(P)H Dependent Enzymes; 2.2.5 Regeneration of NAD(P)H from NAD(P); 2.2.6 Regeneration of NAD(P) from NAD(P)H; 2.3 Direct Electron Transfer between Electrodes and Enzymes; 2.3.1 Enzymes in Solution; 2.3.2 Enzyme-Film Voltammetry: Basic Theory; 2.3.3 Adsorbed and Coadsorbed Enzyme Monolayers
2.3.4 Self-Assembled Monolayers and Covalently Attached Enzymes2.3.5 Enzymes on Carbon Nanotube Electrodes; 2.3.6 Enzymes in Lipid Bilayer Films; 2.3.7 Polyion Films and Layer-by-Layer Methods; 2.4 Outlook for the Future; Acknowledgements; References; 3 Biological Membranes and Membrane Mimics; 3.1 Introduction; 3.2 Membrane Structure and Composition; 3.2.1 Membrane Structure; 3.2.2 Membrane Lipids; 3.2.3 Membrane Proteins; 3.3 Models of Membrane Structure; 3.3.1 Lipid Monolayers; 3.3.2 Bilayer Lipid Membranes (BLM); 3.3.3 Supported Bilayer Lipid Membranes; 3.3.4 Liposomes
3.4 Ordering, Conformation and Molecular Dynamics of Lipid Bilayers3.4.1 Structural Parameters of Lipid Bilayers Measured by X-ray Diffraction; 3.4.2 Interactions between Bilayers; 3.4.3 Dynamics and Order Parameters of Bilayers Determined by EPR and NMR Spectroscopy and by Optical Spectroscopy Methods; 3.5 Phase Transitions of Lipid Bilayers; 3.5.1 Lyotropic and Thermotropic Transitions; 3.5.2 Thermodynamics of Phase Transitions; 3.5.3 Trans-Gauche Isomerization; 3.5.4 Order Parameter; 3.5.5 Cooperativity of Transition; 3.5.6 Theory of Phase Transitions
3.6 Mechanical Properties of Lipid Bilayers
Record Nr. UNINA-9910876694703321
Chichester, England ; ; Hoboken, NJ, : John Wiley & Sons, c2008
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Introductory bioelectronics [[electronic resource] ] : for engineers and physical scientists / / Ronald Pethig, Stewart Smith
Introductory bioelectronics [[electronic resource] ] : for engineers and physical scientists / / Ronald Pethig, Stewart Smith
Autore Pethig Ronald
Pubbl/distr/stampa Chichester, West Sussex ; ; Hoboken, : John Wiley & Sons, 2012
Descrizione fisica xvi, 441 p. : ill
Disciplina 572/.437
Altri autori (Persone) SmithStewart <1975->
Soggetto topico Bioelectronics
Molecular electronics
ISBN 1-118-44339-X
9786613905451
1-283-59300-9
1-118-44329-2
1-118-44328-4
Classificazione 464
572/.437
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910795956303321
Pethig Ronald  
Chichester, West Sussex ; ; Hoboken, : John Wiley & Sons, 2012
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
JACC Clinical Electrophysiology
JACC Clinical Electrophysiology
Pubbl/distr/stampa [New York, N.Y.] : , : Elsevier Inc., , [2015]-
Descrizione fisica 1 online resource
Disciplina 572/.437
Soggetto topico Heart - Electric properties
Electrophysiology
Cardiovascular Diseases
Electrophysiologic Techniques, Cardiac
Soggetto genere / forma Periodical
Periodicals.
ISSN 2405-5018
Formato Materiale a stampa
Livello bibliografico Periodico
Lingua di pubblicazione eng
Altri titoli varianti Clinical Electrophysiology
Journal of the American College of Cardiology
J.A.C.C
Record Nr. UNINA-9910384450303321
[New York, N.Y.] : , : Elsevier Inc., , [2015]-
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Patch clamping [[electronic resource] ] : an introductory guide to patch clamp electrophysiology / / Areles Molleman
Patch clamping [[electronic resource] ] : an introductory guide to patch clamp electrophysiology / / Areles Molleman
Autore Molleman Areles
Pubbl/distr/stampa New York, : J. Wiley, c2003
Descrizione fisica 1 online resource (187 p.)
Disciplina 571.64
572/.437
Soggetto topico Patch-clamp techniques (Electrophysiology)
Soggetto genere / forma Electronic books.
ISBN 1-280-27051-9
9786610270514
0-470-85651-3
0-470-85652-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Patch Clamping; Contents; Preface; 1 Introduction; 1.1 Patch Clamping and its Context; 2 Basic Theoretical Principles; 2.1 Introduction to Membrane Biology; 2.1.1 The plasma membrane and its ionic environment; 2.1.2 Electrochemical gradients and the Nernst equation; 2.1.3 Maintenance of ion gradients and the membrane potential; 2.1.4 Ion channels; 2.2 Electrical Properties of the Cell Membrane; 2.2.1 Driving force and membrane resistance; 2.2.2 Membrane capacitance; 2.2.3 Consequences of membrane capacitance; 2.2.4 An electronic model of the plasma membrane
2.3 Recording Modes and their Equivalent Circuits2.3.1 The basics of equivalent circuits; 2.3.2 Intracellular recording; 2.3.3 Voltage clamp and current clamp; 2.3.4 Introduction to patch clamp configurations; 2.3.5 The equivalent circuit for the cell-attached patch configuration; 2.3.6 The equivalent circuit for the whole-cell configuration; 2.3.7 The equivalent circuit for the excised patch configurations; 3 Requirements; 3.1 The Platform; 3.1.1 Stability: vibrations and drift; 3.1.2 Where in the building should the set-up be placed?; 3.1.3 Anti-vibration tables; 3.2 Mechanics and Optics
3.2.1 The microscope3.2.2 Micromanipulators; 3.2.3 Pipette pressure; 3.2.4 Baths and superfusion systems; 3.3 Electrodes and Micropipettes; 3.3.1 Solid-liquid junction potentials and polarisation; 3.3.2 The bath electrode; 3.3.3 Micropipettes; 3.3.4 Liquid junction potentials; 3.4 Electronics; 3.4.1 External noise and Faraday cages; 3.4.2 Patch clamp amplifiers; 3.4.3 Noise prevention and signal conditioning; 3.4.4 Data acquisition and digitisation; 3.4.5 Computers and software; 4 The Practice of Patch Clamping; 4.1 Preparing the Experiment and Making a Seal; 4.1.1 Setting up
4.1.2 Bringing the pipette near the preparation4.1.3 Making the seal; 4.2 Whole-cell Modes; 4.2.1 Conventional whole-cell recording; 4.2.2 Perforated patch recording; 4.3 Single-channel Modes; 4.3.1 General notes; 4.3.2 Cell-attached patch; 4.3.3 Excised patches; 5 Whole-cell Protocols and Data Analysis; 5.1 Standard Cellular Parameters; 5.2 Voltage-activated Currents; 5.2.1 Introduction to pulse protocols; 5.2.2 Signal conditioning and positive/negative subtraction; 5.2.3 Space clamp artefacts; 5.2.4 Isolation of a homogeneous population of channels
5.2.5 Current-voltage relationships and reversal potential5.2.6 Determination of relative permeabilities; 5.2.7 Activation and inactivation studies; 5.3 Non-voltage-activated Currents; 5.3.1 Introduction to continuous recording; 5.3.2 Determination of reversal potential using voltage ramps; 6 Single-channel Protocols and Data Analysis; 6.1 General Single-channel Practice and Analysis; 6.1.1 Practical notes; 6.1.2 Amplitude analysis; 6.1.3 Event detection; 6.1.4 Dwell time analysis; 6.2 Continuous Recording of Single Channels; 6.2.1 Data acquisition; 6.2.2 Spontaneous activity
6.2.3 Receptor-induced activity
Record Nr. UNINA-9910142497103321
Molleman Areles  
New York, : J. Wiley, c2003
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Patch clamping [[electronic resource] ] : an introductory guide to patch clamp electrophysiology / / Areles Molleman
Patch clamping [[electronic resource] ] : an introductory guide to patch clamp electrophysiology / / Areles Molleman
Autore Molleman Areles
Pubbl/distr/stampa New York, : J. Wiley, c2003
Descrizione fisica 1 online resource (187 p.)
Disciplina 571.64
572/.437
Soggetto topico Patch-clamp techniques (Electrophysiology)
ISBN 1-280-27051-9
9786610270514
0-470-85651-3
0-470-85652-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Patch Clamping; Contents; Preface; 1 Introduction; 1.1 Patch Clamping and its Context; 2 Basic Theoretical Principles; 2.1 Introduction to Membrane Biology; 2.1.1 The plasma membrane and its ionic environment; 2.1.2 Electrochemical gradients and the Nernst equation; 2.1.3 Maintenance of ion gradients and the membrane potential; 2.1.4 Ion channels; 2.2 Electrical Properties of the Cell Membrane; 2.2.1 Driving force and membrane resistance; 2.2.2 Membrane capacitance; 2.2.3 Consequences of membrane capacitance; 2.2.4 An electronic model of the plasma membrane
2.3 Recording Modes and their Equivalent Circuits2.3.1 The basics of equivalent circuits; 2.3.2 Intracellular recording; 2.3.3 Voltage clamp and current clamp; 2.3.4 Introduction to patch clamp configurations; 2.3.5 The equivalent circuit for the cell-attached patch configuration; 2.3.6 The equivalent circuit for the whole-cell configuration; 2.3.7 The equivalent circuit for the excised patch configurations; 3 Requirements; 3.1 The Platform; 3.1.1 Stability: vibrations and drift; 3.1.2 Where in the building should the set-up be placed?; 3.1.3 Anti-vibration tables; 3.2 Mechanics and Optics
3.2.1 The microscope3.2.2 Micromanipulators; 3.2.3 Pipette pressure; 3.2.4 Baths and superfusion systems; 3.3 Electrodes and Micropipettes; 3.3.1 Solid-liquid junction potentials and polarisation; 3.3.2 The bath electrode; 3.3.3 Micropipettes; 3.3.4 Liquid junction potentials; 3.4 Electronics; 3.4.1 External noise and Faraday cages; 3.4.2 Patch clamp amplifiers; 3.4.3 Noise prevention and signal conditioning; 3.4.4 Data acquisition and digitisation; 3.4.5 Computers and software; 4 The Practice of Patch Clamping; 4.1 Preparing the Experiment and Making a Seal; 4.1.1 Setting up
4.1.2 Bringing the pipette near the preparation4.1.3 Making the seal; 4.2 Whole-cell Modes; 4.2.1 Conventional whole-cell recording; 4.2.2 Perforated patch recording; 4.3 Single-channel Modes; 4.3.1 General notes; 4.3.2 Cell-attached patch; 4.3.3 Excised patches; 5 Whole-cell Protocols and Data Analysis; 5.1 Standard Cellular Parameters; 5.2 Voltage-activated Currents; 5.2.1 Introduction to pulse protocols; 5.2.2 Signal conditioning and positive/negative subtraction; 5.2.3 Space clamp artefacts; 5.2.4 Isolation of a homogeneous population of channels
5.2.5 Current-voltage relationships and reversal potential5.2.6 Determination of relative permeabilities; 5.2.7 Activation and inactivation studies; 5.3 Non-voltage-activated Currents; 5.3.1 Introduction to continuous recording; 5.3.2 Determination of reversal potential using voltage ramps; 6 Single-channel Protocols and Data Analysis; 6.1 General Single-channel Practice and Analysis; 6.1.1 Practical notes; 6.1.2 Amplitude analysis; 6.1.3 Event detection; 6.1.4 Dwell time analysis; 6.2 Continuous Recording of Single Channels; 6.2.1 Data acquisition; 6.2.2 Spontaneous activity
6.2.3 Receptor-induced activity
Record Nr. UNINA-9910830071403321
Molleman Areles  
New York, : J. Wiley, c2003
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Patch clamping : an introductory guide to patch clamp electrophysiology / / Areles Molleman
Patch clamping : an introductory guide to patch clamp electrophysiology / / Areles Molleman
Autore Molleman Areles
Pubbl/distr/stampa New York, : J. Wiley, c2003
Descrizione fisica 1 online resource (187 p.)
Disciplina 572/.437
Soggetto topico Patch-clamp techniques (Electrophysiology)
ISBN 1-280-27051-9
9786610270514
0-470-85651-3
0-470-85652-1
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Patch Clamping; Contents; Preface; 1 Introduction; 1.1 Patch Clamping and its Context; 2 Basic Theoretical Principles; 2.1 Introduction to Membrane Biology; 2.1.1 The plasma membrane and its ionic environment; 2.1.2 Electrochemical gradients and the Nernst equation; 2.1.3 Maintenance of ion gradients and the membrane potential; 2.1.4 Ion channels; 2.2 Electrical Properties of the Cell Membrane; 2.2.1 Driving force and membrane resistance; 2.2.2 Membrane capacitance; 2.2.3 Consequences of membrane capacitance; 2.2.4 An electronic model of the plasma membrane
2.3 Recording Modes and their Equivalent Circuits2.3.1 The basics of equivalent circuits; 2.3.2 Intracellular recording; 2.3.3 Voltage clamp and current clamp; 2.3.4 Introduction to patch clamp configurations; 2.3.5 The equivalent circuit for the cell-attached patch configuration; 2.3.6 The equivalent circuit for the whole-cell configuration; 2.3.7 The equivalent circuit for the excised patch configurations; 3 Requirements; 3.1 The Platform; 3.1.1 Stability: vibrations and drift; 3.1.2 Where in the building should the set-up be placed?; 3.1.3 Anti-vibration tables; 3.2 Mechanics and Optics
3.2.1 The microscope3.2.2 Micromanipulators; 3.2.3 Pipette pressure; 3.2.4 Baths and superfusion systems; 3.3 Electrodes and Micropipettes; 3.3.1 Solid-liquid junction potentials and polarisation; 3.3.2 The bath electrode; 3.3.3 Micropipettes; 3.3.4 Liquid junction potentials; 3.4 Electronics; 3.4.1 External noise and Faraday cages; 3.4.2 Patch clamp amplifiers; 3.4.3 Noise prevention and signal conditioning; 3.4.4 Data acquisition and digitisation; 3.4.5 Computers and software; 4 The Practice of Patch Clamping; 4.1 Preparing the Experiment and Making a Seal; 4.1.1 Setting up
4.1.2 Bringing the pipette near the preparation4.1.3 Making the seal; 4.2 Whole-cell Modes; 4.2.1 Conventional whole-cell recording; 4.2.2 Perforated patch recording; 4.3 Single-channel Modes; 4.3.1 General notes; 4.3.2 Cell-attached patch; 4.3.3 Excised patches; 5 Whole-cell Protocols and Data Analysis; 5.1 Standard Cellular Parameters; 5.2 Voltage-activated Currents; 5.2.1 Introduction to pulse protocols; 5.2.2 Signal conditioning and positive/negative subtraction; 5.2.3 Space clamp artefacts; 5.2.4 Isolation of a homogeneous population of channels
5.2.5 Current-voltage relationships and reversal potential5.2.6 Determination of relative permeabilities; 5.2.7 Activation and inactivation studies; 5.3 Non-voltage-activated Currents; 5.3.1 Introduction to continuous recording; 5.3.2 Determination of reversal potential using voltage ramps; 6 Single-channel Protocols and Data Analysis; 6.1 General Single-channel Practice and Analysis; 6.1.1 Practical notes; 6.1.2 Amplitude analysis; 6.1.3 Event detection; 6.1.4 Dwell time analysis; 6.2 Continuous Recording of Single Channels; 6.2.1 Data acquisition; 6.2.2 Spontaneous activity
6.2.3 Receptor-induced activity
Record Nr. UNINA-9910876688703321
Molleman Areles  
New York, : J. Wiley, c2003
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui