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Bioanalytical chemistry [[electronic resource] /] / Susan R. Mikkelsen, Eduardo Cortón
| Bioanalytical chemistry [[electronic resource] /] / Susan R. Mikkelsen, Eduardo Cortón |
| Autore | Mikkelsen Susan R. <1960-> |
| Pubbl/distr/stampa | Hoboken, N.J., : John Wiley & Sons, c2004 |
| Descrizione fisica | 1 online resource (381 p.) |
| Disciplina | 572/.36 |
| Altri autori (Persone) | CortónEduardo <1962-> |
| Soggetto topico | Analytical biochemistry |
| ISBN |
1-280-36794-6
9786610367948 0-470-23229-3 0-471-62386-5 0-471-62362-8 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
BIOANALYTICAL CHEMISTRY; CONTENTS; Preface; Acknowledgments; 1. Spectroscopic Methods for Matrix Characterization; 1.1 Introduction; 1.2 Total Protein; 1.2.1 Lowry Method; 1.2.2 Smith (BCA) Method; 1.2.3 Bradford Method; 1.2.4 Ninhydrin-Based Assay; 1.2.5 Other Protein Quantitation Methods; 1.3 Total DNA; 1.3.1 Diaminobenzoic Acid Method; 1.3.2 Diphenylamine Method; 1.3.3 Other Fluorometric Methods; 1.4 Total RNA; 1.5 Total Carbohydrate; 1.5.1 Ferricyanide Method; 1.5.2 Phenol-Sulfuric Acid Method; 1.5.3 2-Aminothiophenol Method; 1.5.4 Purpald Assay for Bacterial Polysaccharides
1.6 Free Fatty AcidsReferences; Problems; 2. Enzymes; 2.1 Introduction; 2.2 Enzyme Nomenclature; 2.3 Enzyme Commission Numbers; 2.4 Enzymes in Bioanalytical Chemistry; 2.5 Enzyme Kinetics; 2.5.1 Simple One-Substrate Enzyme Kinetics; 2.5.2 Experimental Determination of Michaelis-Menten Parameters; 2.5.2.1 Eadie-Hofstee Method; 2.5.2.2 Hanes Method; 2.5.2.3 Lineweaver-Burk Method; 2.5.2.4 Cornish-Bowden-Eisenthal Method; 2.5.3 Comparison of Methods for the Determination of K(m) Values; 2.5.4 One-Substrate, Two-Product Enzyme Kinetics; 2.5.5 Two-Substrate Enzyme Kinetics 2.5.6 Examples of Enzyme-Catalyzed Reactions and Their Treatment2.6 Enzyme Activators; 2.7 Enzyme Inhibitors; 2.7.1 Competitive Inhibition; 2.7.2 Noncompetitive Inhibition; 2.7.3 Uncompetitive Inhibition; 2.8 Enzyme Units and Concentrations; Suggested References; References; Problems; 3. Quantitation of Enzymes and Their Substrates; 3.1 Introduction; 3.2 Substrate Depletion or Product Accumulation; 3.3 Direct and Coupled Measurements; 3.4 Classification of Methods; 3.5 Instrumental Methods; 3.5.1 Optical Detection; 3.5.1.1 Absorbance; 3.5.1.2 Fluorescence; 3.5.1.3 Luminescence 3.5.1.4 Nephelometry3.5.2 Electrochemical Detection; 3.5.2.1 Amperometry; 3.5.2.2 Potentiometry; 3.5.2.3 Conductimetry; 3.5.3 Other Detection Methods; 3.5.3.1 Radiochemical; 3.5.3.2 Manometry; 3.5.3.3 Calorimetry; 3.6 Ultra-High-Throughput Assays (HTA); 3.7 Practical Considerations for Enzymatic Assays; Suggested References; References; Problems; 4. Immobilized Enzymes; 4.1 Introduction; 4.2 Immobilization Methods; 4.2.1 Nonpolymerizing Covalent Immobilization; 4.2.1.1 Controlled-Pore Glass; 4.2.1.2 Polysaccharides; 4.2.1.3 Polyacrylamide; 4.2.1.4 Acidic Supports; 4.2.1.5 Anhydride Groups 4.2.1.6 Thiol Groups4.2.2 Cross-Linking with Bifunctional Reagents; 4.2.3 Adsorption; 4.2.4 Entrapment; 4.2.5 Microencapsulation; 4.3 Properties of Immobilized Enzymes; 4.4 Immobilized Enzyme Reactors; 4.5 Theoretical Treatment of Packed-Bed Enzyme Reactors; Suggested References; References; Problems; 5. Antibodies; 5.1 Introduction; 5.2 Structural and Functional Properties of Antibodies; 5.3 Polyclonal and Monoclonal Antibodies; 5.4 Antibody-Antigen Interactions; 5.5 Analytical Applications of Secondary Antibody-Antigen Interactions; 5.5.1 Agglutination Reactions 5.5.2 Precipitation Reactions |
| Record Nr. | UNINA-9910780374003321 |
Mikkelsen Susan R. <1960->
|
||
| Hoboken, N.J., : John Wiley & Sons, c2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioanalytical chemistry / / Susan R. Mikkelsen, Eduardo Corton
| Bioanalytical chemistry / / Susan R. Mikkelsen, Eduardo Corton |
| Autore | Mikkelsen Susan R. <1960-> |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : John Wiley & Sons, c2004 |
| Descrizione fisica | 1 online resource (381 p.) |
| Disciplina | 572/.36 |
| Altri autori (Persone) | CortonEduardo <1962-> |
| Soggetto topico | Analytical biochemistry |
| ISBN |
1-280-36794-6
9786610367948 0-470-23229-3 0-471-62386-5 0-471-62362-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
BIOANALYTICAL CHEMISTRY; CONTENTS; Preface; Acknowledgments; 1. Spectroscopic Methods for Matrix Characterization; 1.1 Introduction; 1.2 Total Protein; 1.2.1 Lowry Method; 1.2.2 Smith (BCA) Method; 1.2.3 Bradford Method; 1.2.4 Ninhydrin-Based Assay; 1.2.5 Other Protein Quantitation Methods; 1.3 Total DNA; 1.3.1 Diaminobenzoic Acid Method; 1.3.2 Diphenylamine Method; 1.3.3 Other Fluorometric Methods; 1.4 Total RNA; 1.5 Total Carbohydrate; 1.5.1 Ferricyanide Method; 1.5.2 Phenol-Sulfuric Acid Method; 1.5.3 2-Aminothiophenol Method; 1.5.4 Purpald Assay for Bacterial Polysaccharides
1.6 Free Fatty AcidsReferences; Problems; 2. Enzymes; 2.1 Introduction; 2.2 Enzyme Nomenclature; 2.3 Enzyme Commission Numbers; 2.4 Enzymes in Bioanalytical Chemistry; 2.5 Enzyme Kinetics; 2.5.1 Simple One-Substrate Enzyme Kinetics; 2.5.2 Experimental Determination of Michaelis-Menten Parameters; 2.5.2.1 Eadie-Hofstee Method; 2.5.2.2 Hanes Method; 2.5.2.3 Lineweaver-Burk Method; 2.5.2.4 Cornish-Bowden-Eisenthal Method; 2.5.3 Comparison of Methods for the Determination of K(m) Values; 2.5.4 One-Substrate, Two-Product Enzyme Kinetics; 2.5.5 Two-Substrate Enzyme Kinetics 2.5.6 Examples of Enzyme-Catalyzed Reactions and Their Treatment2.6 Enzyme Activators; 2.7 Enzyme Inhibitors; 2.7.1 Competitive Inhibition; 2.7.2 Noncompetitive Inhibition; 2.7.3 Uncompetitive Inhibition; 2.8 Enzyme Units and Concentrations; Suggested References; References; Problems; 3. Quantitation of Enzymes and Their Substrates; 3.1 Introduction; 3.2 Substrate Depletion or Product Accumulation; 3.3 Direct and Coupled Measurements; 3.4 Classification of Methods; 3.5 Instrumental Methods; 3.5.1 Optical Detection; 3.5.1.1 Absorbance; 3.5.1.2 Fluorescence; 3.5.1.3 Luminescence 3.5.1.4 Nephelometry3.5.2 Electrochemical Detection; 3.5.2.1 Amperometry; 3.5.2.2 Potentiometry; 3.5.2.3 Conductimetry; 3.5.3 Other Detection Methods; 3.5.3.1 Radiochemical; 3.5.3.2 Manometry; 3.5.3.3 Calorimetry; 3.6 Ultra-High-Throughput Assays (HTA); 3.7 Practical Considerations for Enzymatic Assays; Suggested References; References; Problems; 4. Immobilized Enzymes; 4.1 Introduction; 4.2 Immobilization Methods; 4.2.1 Nonpolymerizing Covalent Immobilization; 4.2.1.1 Controlled-Pore Glass; 4.2.1.2 Polysaccharides; 4.2.1.3 Polyacrylamide; 4.2.1.4 Acidic Supports; 4.2.1.5 Anhydride Groups 4.2.1.6 Thiol Groups4.2.2 Cross-Linking with Bifunctional Reagents; 4.2.3 Adsorption; 4.2.4 Entrapment; 4.2.5 Microencapsulation; 4.3 Properties of Immobilized Enzymes; 4.4 Immobilized Enzyme Reactors; 4.5 Theoretical Treatment of Packed-Bed Enzyme Reactors; Suggested References; References; Problems; 5. Antibodies; 5.1 Introduction; 5.2 Structural and Functional Properties of Antibodies; 5.3 Polyclonal and Monoclonal Antibodies; 5.4 Antibody-Antigen Interactions; 5.5 Analytical Applications of Secondary Antibody-Antigen Interactions; 5.5.1 Agglutination Reactions 5.5.2 Precipitation Reactions |
| Record Nr. | UNINA-9910815439603321 |
|
Mikkelsen Susan R. <1960->
|
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| Hoboken, N.J., : John Wiley & Sons, c2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Electrospray and MALDI mass spectrometry [[electronic resource] ] : fundamentals, instrumentation, practicalities, and biological applications / / edited by Richard B. Cole
| Electrospray and MALDI mass spectrometry [[electronic resource] ] : fundamentals, instrumentation, practicalities, and biological applications / / edited by Richard B. Cole |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, c2010 |
| Descrizione fisica | 1 online resource (896 p.) |
| Disciplina | 572/.36 |
| Altri autori (Persone) | ColeRichard B. <1956-> |
| Soggetto topico |
Electrospray ionization mass spectrometry
Biomolecules - Analysis |
| ISBN |
1-118-21155-3
0-470-58890-X 1-282-72870-9 9786612728709 0-470-58889-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt. 1. Fundamentals of ES -- pt. 2. Fundamentals of MALDI -- pt. 3. ES and MALDI coupling to mass spectrometry instrumentation -- pt. 4. Practical aspects of ES and MALDI -- pt. 5. Biological applications of ES and MALDI. |
| Record Nr. | UNINA-9910140834503321 |
| Hoboken, N.J., : Wiley, c2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Electrospray and MALDI mass spectrometry : fundamentals, instrumentation, practicalities, and biological applications / / edited by Richard B. Cole
| Electrospray and MALDI mass spectrometry : fundamentals, instrumentation, practicalities, and biological applications / / edited by Richard B. Cole |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, c2010 |
| Descrizione fisica | 1 online resource (896 p.) |
| Disciplina | 572/.36 |
| Altri autori (Persone) | ColeRichard B. <1956-> |
| Soggetto topico |
Electrospray ionization mass spectrometry
Biomolecules - Analysis |
| ISBN |
1-118-21155-3
0-470-58890-X 1-282-72870-9 9786612728709 0-470-58889-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt. 1. Fundamentals of ES -- pt. 2. Fundamentals of MALDI -- pt. 3. ES and MALDI coupling to mass spectrometry instrumentation -- pt. 4. Practical aspects of ES and MALDI -- pt. 5. Biological applications of ES and MALDI. |
| Record Nr. | UNINA-9910809387603321 |
| Hoboken, N.J., : Wiley, c2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Introduction to mass spectrometry of biomolecules : theory and principles / / Marko Jovanovic
| Introduction to mass spectrometry of biomolecules : theory and principles / / Marko Jovanovic |
| Autore | Jovanović Marko <1976-> |
| Pubbl/distr/stampa | New York, [New York] : , : Nova Science Publishers, Inc., , 2016 |
| Descrizione fisica | 1 online resource (301 pages) |
| Disciplina | 572/.36 |
| Collana | Chemistry Research and Applications |
| Soggetto topico |
Mass spectrometry
Biomolecules - Analysis |
| ISBN | 1-63485-142-0 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910136567003321 |
|
Jovanović Marko <1976->
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||
| New York, [New York] : , : Nova Science Publishers, Inc., , 2016 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Mass spectrometry and stable isotopes in nutritional and pediatric research / / edited by Henk Schierbeek
| Mass spectrometry and stable isotopes in nutritional and pediatric research / / edited by Henk Schierbeek |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : John Wiley & Sons, Incorporated, , [2017] |
| Descrizione fisica | 1 online resource (432 pages) : illustrations (some color) |
| Disciplina | 572/.36 |
| Collana | Wiley series on mass spectrometry |
| Soggetto topico |
Biomolecules - Analysis
Mass spectrometry Nutrition - Research Pediatrics - Research |
| ISBN |
1-119-34118-3
1-119-34112-4 1-119-34124-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910162810303321 |
| Hoboken, New Jersey : , : John Wiley & Sons, Incorporated, , [2017] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Mass spectrometry and stable isotopes in nutritional and pediatric research / / edited by Henk Schierbeek
| Mass spectrometry and stable isotopes in nutritional and pediatric research / / edited by Henk Schierbeek |
| Pubbl/distr/stampa | Hoboken, New Jersey : , : John Wiley & Sons, Incorporated, , [2017] |
| Descrizione fisica | 1 online resource (432 pages) : illustrations (some color) |
| Disciplina | 572/.36 |
| Collana | Wiley series on mass spectrometry |
| Soggetto topico |
Biomolecules - Analysis
Mass spectrometry Nutrition - Research Pediatrics - Research |
| ISBN |
1-119-34118-3
1-119-34112-4 1-119-34124-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910820696903321 |
| Hoboken, New Jersey : , : John Wiley & Sons, Incorporated, , [2017] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Mass spectrometry for microbial proteomics [[electronic resource] /] / edited by Haroun Shah and Saheer Gharbia
| Mass spectrometry for microbial proteomics [[electronic resource] /] / edited by Haroun Shah and Saheer Gharbia |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, 2010 |
| Descrizione fisica | 1 online resource (535 p.) |
| Disciplina | 572/.36 |
| Altri autori (Persone) |
ShahHaroun N
GharbiaSaheer |
| Soggetto topico |
Microbial proteins - Spectra
Mass spectrometry Proteomics - Methodology |
| ISBN |
1-119-99192-7
1-282-65384-9 9786612653841 0-470-66549-1 0-470-66547-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt. 1. Microbial characterisation ; The transition from conventional methods to proteomics -- pt. 2. Proteomics tools and biomarker discovery -- pt. 3. Protein samples : preparation techniques -- pt. 4. Characterisation of microorganisms by pattern matching of mass spectral profiles and biomarker approaches requiring minimal sample preparation -- pt. 5. Targeted molecules and analysis of specific microorganisms -- pt. 6. Statistical analysis of 2D gels and analysis of mass spectral data -- pt. 7. DNA resequencing by MALDI-TOF-mass spectrometry and its application to traditional microbiological problems. |
| Record Nr. | UNINA-9910140555103321 |
| Hoboken, N.J., : Wiley, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Mass spectrometry for microbial proteomics / / edited by Haroun Shah and Saheer Gharbia
| Mass spectrometry for microbial proteomics / / edited by Haroun Shah and Saheer Gharbia |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, 2010 |
| Descrizione fisica | 1 online resource (535 p.) |
| Disciplina | 572/.36 |
| Altri autori (Persone) |
ShahHaroun N
GharbiaSaheer |
| Soggetto topico |
Microbial proteins - Spectra
Mass spectrometry Proteomics - Methodology |
| ISBN |
1-119-99192-7
1-282-65384-9 9786612653841 0-470-66549-1 0-470-66547-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | pt. 1. Microbial characterisation ; The transition from conventional methods to proteomics -- pt. 2. Proteomics tools and biomarker discovery -- pt. 3. Protein samples : preparation techniques -- pt. 4. Characterisation of microorganisms by pattern matching of mass spectral profiles and biomarker approaches requiring minimal sample preparation -- pt. 5. Targeted molecules and analysis of specific microorganisms -- pt. 6. Statistical analysis of 2D gels and analysis of mass spectral data -- pt. 7. DNA resequencing by MALDI-TOF-mass spectrometry and its application to traditional microbiological problems. |
| Record Nr. | UNINA-9910827526003321 |
| Hoboken, N.J., : Wiley, 2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Preparation of compounds labeled with tritium and carbon-14 [[electronic resource] /] / Rolf Voges, J. Richard Heys, Thomas Moenius
| Preparation of compounds labeled with tritium and carbon-14 [[electronic resource] /] / Rolf Voges, J. Richard Heys, Thomas Moenius |
| Autore | Voges R (Rolf) |
| Pubbl/distr/stampa | Chichester, United Kingdom, : Wiley, 2009 |
| Descrizione fisica | 1 online resource (684 p.) |
| Disciplina |
547.2
572.36 572/.36 |
| Altri autori (Persone) |
HeysJ. R (J. Richard)
MoeniusThomas |
| Soggetto topico |
Organic compounds - Synthesis
Radiolabeling Tritium Carbon - Isotopes |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-282-13816-2
9786612138164 0-470-74344-1 0-470-74343-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Preparation of Compounds Labeled with Tritium and Carbon-14; Contents; Preface; Glossary; Author Biographies; 1 Introduction; 1.1 Physical Properties of Tritium and Carbon-14; 1.2 Purification; 1.3 Analysis; 1.3.1 Chemical Identity; 1.3.2 Chemical (and Enantiomeric) Purity; 1.3.3 Radiochemical (and Radionuclidic) Purity; 1.3.4 Specific Activity; 1.3.5 Position of Label; 1.4 Stability and Storage of Compounds Labeled with Tritium or Carbon-14; 1.5 Specialist Techniques and Equipment; References; 2 Strategies for Target Preparation; 2.1 Formulating Target Specifications
2.2 Planning Radiotracer Preparations2.2.1 The Construction Strategy; 2.2.2 Reconstitution Strategies; 2.2.3 The Derivatization Strategy; 2.2.4 Selection of an Appropriate Strategy; 2.2.5 Case Studies of Strategy Development; References; 3 Preparation of Tritium-Labeled Compounds by Isotope Exchange Reactions; 3.1 Homogeneous Acid- or Base-Catalyzed Exchange; 3.1.1 Exchange without Added Acid or Base; 3.1.2 Exchange under Acidic Conditions; 3.1.3 Exchange under Basic Conditions; 3.2 Heterogeneous Catalysis with Tritium in Solvent; 3.2.1 Metals; 3.2.2 Other Catalysts 3.3 Heterogeneous Catalysis in Solution with Tritium Gas3.3.1 Metal Catalysts with Nonreducible Substrates in Aqueous Solution; 3.3.2 Metal Catalysts with Nonreducible Substrates in Organic Solvents; 3.3.3 Other Catalysts; 3.3.4 Metal Catalysts with Reducible Substrates; 3.4 Homogeneous Catalysis in Solution with Tritiated Water; 3.4.1 Exchange Catalyzed by Metal Salts; 3.4.2 Exchange Catalyzed by Organoruthenium Complexes; 3.4.3 Exchange Catalyzed by Iridium Dionates; 3.4.4 Exchange Catalyzed by Iridium Cyclopentadienides; 3.5 Homogeneous Catalysis with Tritium Gas; 3.5.1 Iridium Phosphines 3.5.2 Iridium Dionate Complexes3.5.3 Iridium Cyclopentadienide Complexes; 3.6 Solvent-Free Catalytic Exchange; 3.6.1 High-Temperature Solid-State Catalytic Isotope Exchange; 3.6.2 Thermal Tritium Atom Bombardment; 3.6.3 Other Radiation-Induced Labeling Methods; References; 4 Preparation of Tritium-Labeled Compounds by Chemical Synthesis; 4.1 Catalytic Tritiations; 4.1.1 Tritiation of Carbon-Carbon Multiple Bonds; 4.1.2 Tritiation of Carbon-Heteroatom Multiple Bonds; 4.1.3 Homogeneously Catalyzed Reactions; 4.2 Catalytic Tritiolyses; 4.2.1 Tritiodehalogenations 4.2.2 Tritiolyses of Benzylic N- and O-Functions4.2.3 Tritiodesulfurizations; 4.3 Tritide Reductions; 4.3.1 Sodium Borotritide (NaB3H4 ); 4.3.2 Sodium Cyanoborotritide (NaB3H3CN); 4.3.3 Sodium/Tetramethylammonium Triacetoxyborotritide [Na/NMe4B3H(OAc)3]; 4.3.4 Lithium Tritide (Li3H); 4.3.5 Lithium Borotritide (LiB3H4); 4.3.6 Lithium Triethylborotritide (LiEt3B3H, Li-Super-Tritide); 4.3.7 Lithium Tri-sec-Butylborotritide [Li(sec-Bu3)B3H, Li T-Selectride]; 4.3.8 Lithium [3H2]Boratabicyclo[3.3.1]nonane; 4.3.9 Tritiated Borane (THF-Complex) (B2 3H6; B3H3 .THF); 4.3.10 Tritiated Alkylboranes 4.3.11 Lithium Aluminum Tritide (LiAl3H4) |
| Record Nr. | UNINA-9910146144203321 |
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Voges R (Rolf)
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| Chichester, United Kingdom, : Wiley, 2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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