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Culture of animal cells : a manual of basic technique and specialized applications / / R. Ian Freshney
| Culture of animal cells : a manual of basic technique and specialized applications / / R. Ian Freshney |
| Autore | Freshney R. Ian |
| Edizione | [6th ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley-Blackwell, c2010 |
| Descrizione fisica | 1 online resource (766 p.) |
| Disciplina |
571.6/381
571.6381 |
| Soggetto topico |
Tissue culture
Cell culture |
| ISBN |
1-283-02488-8
9786613024886 0-470-64936-4 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Culture of Animal Cells; Contents; List of Figures; List of Color Plates; List of Protocols; Preface and Acknowledgements; Abbreviations; 1. Introduction; 1.1. Historical Background; 1.2. Advantages of Tissue Culture; 1.2.1. Control of the Environment; 1.2.2. Characterization and Homogeneity of Samples; 1.2.3. Economy, Scale, and Mechanization; 1.2.4. In vitro Modeling of In vivo Conditions; 1.3. Limitations; 1.3.1. Expertise; 1.3.2. Quantity; 1.3.3. Dedifferentiation and Selection; 1.3.4. Origin of Cells; 1.3.5. Instability; 1.4. Major Differences In vitro; 1.5. Types of Tissue Culture
2. Biology of Cultured Cells2.1. The Culture Environment; 2.2. Cell Adhesion; 2.2.1. Cell Adhesion Molecules; 2.2.2. Intercellular Junctions; 2.2.3. Extracellular Matrix; 2.2.4. Cytoskeleton; 2.2.5. Cell Motility; 2.3. Cell Proliferation; 2.3.1. Cell Cycle; 2.3.2. Control of Cell Proliferation; 2.4. Differentiation; 2.4.1. Maintenance of Differentiation; 2.4.2. Dedifferentiation; 2.5. Cell Signaling; 2.6. Energy Metabolism; 2.7. Origin of Cultured Cells; 2.7.1. Initiation of the Culture; 2.7.2. Evolution of Cell Lines; 2.7.3. Senescence 2.7.4. Transformation and the Development of Continuous Cell Lines3. Laboratory Design, Layout, and Equipment; 3.1. Layout, Furnishing, and Services; 3.1.1. Requirements; 3.1.2. Services; 3.1.3. Ventilation; 3.2. Layout; 3.2.1. Sterile Handling Area; 3.2.2. Laminar Flow; 3.2.3. Service Bench; 3.2.4. Quarantine and Containment; 3.2.5. Incubation; 3.2.6. Preparation Area; 3.2.7. Storage; 4. Equipment and Materials; 4.1. Requirements of a Tissue Culture Laboratory; 4.2. Aseptic Area; 4.2.1. Laminar-Flow Hood; 4.2.2. Service Carts; 4.2.3. Sterile Liquid Handling-Pipetting and Dispensing 4.2.4. Inverted Microscope4.2.5. CCD Camera and Monitor; 4.2.6. Dissecting Microscope; 4.2.7. Centrifuge; 4.2.8. Cell Counting; 4.3. Incubation and Culture; 4.3.1. Incubator; 4.3.2. Humid CO2 Incubator; 4.3.3. Temperature Recorder; 4.3.4. Roller Racks; 4.3.5. Magnetic Stirrer; 4.3.6. Culture Vessels; 4.4. Preparation and Sterilization; 4.4.1. Washup; 4.4.2. Preparation of Media and Reagents; 4.4.3. Sterilization; 4.5. Storage; 4.5.1. Consumables; 4.5.2. Refrigerators and Freezers; 4.5.3. Cryostorage Containers; 4.5.4. Controlled-Rate Freezer; 4.6. Supplementary Laboratory Equipment 4.6.1. Computers and Networks4.6.2. Upright Microscope; 4.6.3. Low-Temperature Freezer; 4.6.4. Confocal Microscope; 4.6.5. PCR Thermal Cycler; 4.7. Specialized Equipment; 4.7.1. Microinjection Facilities; 4.7.2. Colony Counter; 4.7.3. Centrifugal Elutriator; 4.7.4. Flow Cytometer; 5. Aseptic Technique; 5.1. Objectives of Aseptic Technique; 5.1.1. Risk of Contamination; 5.1.2. Maintaining Sterility; 5.2. Elements of Aseptic Environment; 5.2.1. Laminar Flow; 5.2.2. Quiet Area; 5.2.3. Work Surface; 5.2.4. Personal Hygiene; 5.2.5. Reagents and Media; 5.2.6. Cultures; 5.3. Sterile Handling 5.3.1. Swabbing |
| Record Nr. | UNINA-9910133601903321 |
Freshney R. Ian
|
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| Hoboken, N.J., : Wiley-Blackwell, c2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Culture of animal cells : a manual of basic technique / R. Ian Freshney
| Culture of animal cells : a manual of basic technique / R. Ian Freshney |
| Autore | Freshney, R. Ian |
| Edizione | [5th ed.] |
| Pubbl/distr/stampa | Hoboken, N. J. : Wiley-Liss, c2005 |
| Descrizione fisica | 642 p. : ill. (some col.) ; 29 cm |
| Disciplina | 571.6381 |
| Soggetto topico |
Tissue culture - Laboratory manuals
Cell culture - Laboratory manuals |
| ISBN | 0471453293 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991001451749707536 |
|
Freshney, R. Ian
|
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| Hoboken, N. J. : Wiley-Liss, c2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
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