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Flow cytometry [[electronic resource] ] : first principles / / Alice Longobardi Givan
| Flow cytometry [[electronic resource] ] : first principles / / Alice Longobardi Givan |
| Autore | Givan Alice Longobardi |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | New York, : Wiley-Liss, c2001 |
| Descrizione fisica | 1 online resource (292 p.) |
| Disciplina |
571.6/028
571.60282 |
| Soggetto topico |
Flow cytometry
Cytology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-118-68839-2
1-280-54171-7 9786610541713 0-471-45911-9 0-471-22394-8 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Contents; Preface; Acknowledgments (First Edition); Acknowledgments (Second Edition); 1 The Past as Prologue; 2 Setting the Scene; 3 Instrumentation: Into the Black Box; Illumination of the Stream; Centering Cells in the Illuminating Beam; Detection of Signals from Cells; Electronics; 4 Information: Harnessing the Data; Data Storage; Data Analysis; 5 Seeing the Light: Lasers, Fluorochromes, and Filters; General Theory; Lasers; Fluorochromes; Partitioning the Signal with Lenses, Filters, and Mirrors; Spectral Compensation
6 Cells from Without: Leukocytes, Surface Proteins, and the Strategy of GatingCells from Blood; Staining for Surface Markers; Controls; Quantitation; Sensitivity; The Strategy of Gating; Gating on Fluorescence; 7 Cells from Within: Intracellular Proteins; Methods for Permeabilizing Cells; Examples of Intracellular Staining; 8 Cells from Within: DNA in Life and Death; Fluorochromes for DNA Analysis; Ploidy; Cell Cycle Analysis; Two-Color Analysis for DNA and Another Parameter; Chromosomes; Apoptosis; Necrosis; 9 The Sorting of Cells; Sorting Theory; Characterization of Sorted Cells Alternative Methods for SortingThe Condition of Cells After Sorting; 10 Disease and Diagnosis: The Clinical Laboratory; The Hematology Laboratory; The Pathology Laboratory; Solid Organ Transplantation; Comments; 11 Out of the Mainstream: Research Frontiers; Functional Assays; The Aquatic Environment; Reporter Molecules; Microbiology; Molecular Biology; Multiplex Cytometry for Soluble Analytes; Reproductive Technology; 12 Flowing On: The Future; General References; Glossary; A; B; C; D; E; F; G; H; I; L; M; N; O; P; Q; R; S; T; V; W; Figure Credits; Index; A; B; C; D; E; F; G; H; I; J; K; L; M NO; P; Q; R; S; T; U; V; W; X; Y; Z |
| Record Nr. | UNINA-9910143186703321 |
Givan Alice Longobardi
|
||
| New York, : Wiley-Liss, c2001 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Flow cytometry [[electronic resource] ] : first principles / / Alice Longobardi Givan
| Flow cytometry [[electronic resource] ] : first principles / / Alice Longobardi Givan |
| Autore | Givan Alice Longobardi |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | New York, : Wiley-Liss, c2001 |
| Descrizione fisica | 1 online resource (292 p.) |
| Disciplina |
571.6/028
571.60282 |
| Soggetto topico |
Flow cytometry
Cytology |
| ISBN |
1-118-68839-2
1-280-54171-7 9786610541713 0-471-45911-9 0-471-22394-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Contents; Preface; Acknowledgments (First Edition); Acknowledgments (Second Edition); 1 The Past as Prologue; 2 Setting the Scene; 3 Instrumentation: Into the Black Box; Illumination of the Stream; Centering Cells in the Illuminating Beam; Detection of Signals from Cells; Electronics; 4 Information: Harnessing the Data; Data Storage; Data Analysis; 5 Seeing the Light: Lasers, Fluorochromes, and Filters; General Theory; Lasers; Fluorochromes; Partitioning the Signal with Lenses, Filters, and Mirrors; Spectral Compensation
6 Cells from Without: Leukocytes, Surface Proteins, and the Strategy of GatingCells from Blood; Staining for Surface Markers; Controls; Quantitation; Sensitivity; The Strategy of Gating; Gating on Fluorescence; 7 Cells from Within: Intracellular Proteins; Methods for Permeabilizing Cells; Examples of Intracellular Staining; 8 Cells from Within: DNA in Life and Death; Fluorochromes for DNA Analysis; Ploidy; Cell Cycle Analysis; Two-Color Analysis for DNA and Another Parameter; Chromosomes; Apoptosis; Necrosis; 9 The Sorting of Cells; Sorting Theory; Characterization of Sorted Cells Alternative Methods for SortingThe Condition of Cells After Sorting; 10 Disease and Diagnosis: The Clinical Laboratory; The Hematology Laboratory; The Pathology Laboratory; Solid Organ Transplantation; Comments; 11 Out of the Mainstream: Research Frontiers; Functional Assays; The Aquatic Environment; Reporter Molecules; Microbiology; Molecular Biology; Multiplex Cytometry for Soluble Analytes; Reproductive Technology; 12 Flowing On: The Future; General References; Glossary; A; B; C; D; E; F; G; H; I; L; M; N; O; P; Q; R; S; T; V; W; Figure Credits; Index; A; B; C; D; E; F; G; H; I; J; K; L; M NO; P; Q; R; S; T; U; V; W; X; Y; Z |
| Record Nr. | UNINA-9910678158303321 |
|
Givan Alice Longobardi
|
||
| New York, : Wiley-Liss, c2001 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||