A manual of applied techniques for biological electron microscopy / Michael J. Dykstra |
Autore | Dykstra, Michael J. |
Pubbl/distr/stampa | New York ; London : Plenum, 1993 |
Descrizione fisica | XIII, 257 p. : ill. ; 23 cm. |
Disciplina | 570.2825(Microscopia elettronica) |
ISBN | 03-06-44449-6 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNICAMPANIA-SUN0064387 |
Dykstra, Michael J. | ||
New York ; London : Plenum, 1993 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Vanvitelli | ||
|
A manual of applied techniques for biological electron microscopy / Michael J. Dykstra |
Autore | Dykstra, Michael J. |
Pubbl/distr/stampa | New York ; London, : Plenum, 1993 |
Descrizione fisica | XIII, 257 p. : ill. ; 23 cm |
Disciplina | 570.2825(Microscopia elettronica) |
ISBN | 03-06-44449-6 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNICAMPANIA-VAN0064387 |
Dykstra, Michael J. | ||
New York ; London, : Plenum, 1993 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Vanvitelli | ||
|
Biological electron microscopy : theory, techniques, and troubleshooting / Michael J. Dykstra |
Autore | Dykstra, Michael J. |
Pubbl/distr/stampa | New York : Plenum, 1992 |
Descrizione fisica | xviii, 360 p. ; 24 cm. |
Disciplina | 570.2825(Microscopia elettronica) |
ISBN | 03-06-44277-9 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNICAMPANIA-SUN0018503 |
Dykstra, Michael J. | ||
New York : Plenum, 1992 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Vanvitelli | ||
|
Biological electron microscopy : theory, techniques, and troubleshooting / Michael J. Dykstra |
Autore | Dykstra, Michael J. |
Pubbl/distr/stampa | New York, : Plenum, 1992 |
Descrizione fisica | xviii, 360 p. ; 24 cm. |
Disciplina | 570.2825(Microscopia elettronica) |
ISBN | 03-06-44277-9 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Record Nr. | UNICAMPANIA-VAN0018503 |
Dykstra, Michael J. | ||
New York, : Plenum, 1992 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Vanvitelli | ||
|
Biological low-voltage scanning electron microscopy [[electronic resource] /] / edited by Heide Schatten, James B. Pawley |
Edizione | [1st ed. 2008.] |
Pubbl/distr/stampa | New York, : Springer, c2008 |
Descrizione fisica | 1 online resource (328 p.) |
Disciplina | 570.2825 |
Altri autori (Persone) |
SchattenHeide
PawleyJames B |
Soggetto topico |
Low-voltage scanning electron microscopy
Field emission cathodes Scanning electron microscopes |
Soggetto genere / forma | Electronic books. |
ISBN |
1-281-13990-4
9786611139902 0-387-72972-0 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | The Early Development of the Scanning Electron Microscope -- LVSEM for Biology -- The Aberration-Corrected SEM -- Noise and Its Effects on the Low-Voltage SEM -- High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols -- Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM -- Low kV and Video-Rate, Beam-Tilt Stereo for Viewing Live-Time Experiments in the SEM -- Cryo-SEM of Chemically Fixed Animal Cells -- High-Resolution and Low-Voltage SEM of Plant Cells -- High-Resolution Cryoscanning Electron Microscopy of Biological Samples -- Developments in Instrumentation for Microanalysis in Low-Voltage Scanning Electron Microscopy. |
Record Nr. | UNINA-9910451694003321 |
New York, : Springer, c2008 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Biological low-voltage scanning electron microscopy [[electronic resource] /] / edited by Heide Schatten, James B. Pawley |
Edizione | [1st ed. 2008.] |
Pubbl/distr/stampa | New York, : Springer, c2008 |
Descrizione fisica | 1 online resource (328 p.) |
Disciplina | 570.2825 |
Altri autori (Persone) |
SchattenHeide
PawleyJames B |
Soggetto topico |
Low-voltage scanning electron microscopy
Field emission cathodes Scanning electron microscopes |
ISBN |
1-281-13990-4
9786611139902 0-387-72972-0 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | The Early Development of the Scanning Electron Microscope -- LVSEM for Biology -- The Aberration-Corrected SEM -- Noise and Its Effects on the Low-Voltage SEM -- High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols -- Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM -- Low kV and Video-Rate, Beam-Tilt Stereo for Viewing Live-Time Experiments in the SEM -- Cryo-SEM of Chemically Fixed Animal Cells -- High-Resolution and Low-Voltage SEM of Plant Cells -- High-Resolution Cryoscanning Electron Microscopy of Biological Samples -- Developments in Instrumentation for Microanalysis in Low-Voltage Scanning Electron Microscopy. |
Record Nr. | UNINA-9910782096403321 |
New York, : Springer, c2008 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Biological low-voltage scanning electron microscopy [[electronic resource] /] / edited by Heide Schatten, James B. Pawley |
Edizione | [1st ed. 2008.] |
Pubbl/distr/stampa | New York, : Springer, c2008 |
Descrizione fisica | 1 online resource (328 p.) |
Disciplina | 570.2825 |
Altri autori (Persone) |
SchattenHeide
PawleyJames B |
Soggetto topico |
Low-voltage scanning electron microscopy
Field emission cathodes Scanning electron microscopes |
ISBN |
1-281-13990-4
9786611139902 0-387-72972-0 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto | The Early Development of the Scanning Electron Microscope -- LVSEM for Biology -- The Aberration-Corrected SEM -- Noise and Its Effects on the Low-Voltage SEM -- High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols -- Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM -- Low kV and Video-Rate, Beam-Tilt Stereo for Viewing Live-Time Experiments in the SEM -- Cryo-SEM of Chemically Fixed Animal Cells -- High-Resolution and Low-Voltage SEM of Plant Cells -- High-Resolution Cryoscanning Electron Microscopy of Biological Samples -- Developments in Instrumentation for Microanalysis in Low-Voltage Scanning Electron Microscopy. |
Record Nr. | UNINA-9910811183403321 |
New York, : Springer, c2008 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Biomedical electron microscopy [[electronic resource] ] : illustrated methods and interpretations / / Arvid B. Maunsbach, Björn A. Afzelius |
Autore | Maunsbach Arvid Bernhard |
Pubbl/distr/stampa | San Diego, CA, : Academic Press, c1999 |
Descrizione fisica | 1 online resource (569 p.) |
Disciplina |
502.825
570.2825 570/.28/25 21 |
Altri autori (Persone) | AfzeliusBjörn |
Soggetto topico |
Electron microscopy - Technique
Medical microscopy - Technique |
Soggetto genere / forma | Electronic books. |
ISBN |
1-281-27948-X
9786611279486 0-08-052809-0 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Front Cover; BIOMEDICAL ELECTRON MICROSCOPY; Copright Page; FOREWORD; PREFACE; ACKNOWLEDGMENTS; CHAPTER 1. MICROGRAPH INTERPRETATION; 1. Classical Preparation Method; 2. Low Temperature Approach; 3. A Common Test Specimen; 4. Detection of Objects; 5. Identification of Artifacts; 6. Analysis of Geometry; 7. Biological Identification; 8. Biological Diversity; 9. Analysis of Dynamics: Endocytosis; 10. Analysis of Dynamics: Synthesis; 11. Comparison of Methods; 12. Variations in Magnifications; 13. Interpretation Difficulties; 14. Diagnostic Pathology; CHAPTER 2. FIXATIVES
1. Osmium Tetroxide and Glutaraldehyde at Low Magnification2. Osmium Tetroxide and Glutaraldehyde at High Magnification; 3. Glutaraldehyde Concentration: Perfusion Fixation; 4. Glutaraldehyde Concentration: Immersion Fixation; 5. Long Fixation Times; 6. Formaldehyde-Glutaraldehyde Combinations; 7. Potassium Permanganate, Picric Acid, and Ruthenium Red; 8. Lead Salts and Tannic Acid; 9. Uranyl Acetate Postfixation; 10. Tannic Acid-Uranyl Acetate Variations; 11. Osmium Tetroxide-Potassium Ferrocyanide; 12. Osmium Tetroxide Artifacts; 13. Glutaraldehyde Artifacts; CHAPTER 3. FIXATIVE VEHICLE 1. Absence and Presence of Buffer2. Comparison of Buffers; 3. Osmolality of Perfusion Fixatives; 4. Effects of Osmolality on Cell Shape; 5. Effects of Osmolality on Cell Organelles; 6. Adjustment of Osmolality with Sucrose; 7. Colloid Osmotic Pressure: Low Magnification; 8. Colloid Osmotic Pressure: High Magnification; 9. Phosphate Buffer Precipitate; CHAPTER 4. FIXATIVE APPLICATION; 1. Perfusion-Fixation versus Immersion-Fixation; 2. Perfusion-Fixation with Pressure Control; 3. Fixation by Dripping in Vivo; 4. Immersion-Fixation; 5. Variability within the Tissue 6. Unsuccessful Perfusion-Fixation7. Superficial Tissue Damage; 8. Early Postmortal Changes; 9. Late Postmortal Changes; 10. Influence of Biopsy Method; 11. Microwave Treatment; CHAPTER 5. DEHYDRATION AND EMBEDDING; 1. Stepwise versus Direct Dehydration; 2. Prolonged Dehydration in Ethanol; 3. Prolonged Dehydration in Acetone; 4. Inert Dehydration; 5. Choice of Intermediate Solvent; 6. Epon, Araldite, and Vestopal: Unstained Sections; 7. Epon, Araldite, and Vestopal: Stained Sections; 8. Different Brands of Epoxy Resins; 9. Spurr and LR White; 10. Embedding of Isolated Cells CHAPTER 6. FREEZING AND LOW-TEMPERATURE EMBEDDING1. Plunge Freezing; 2. Contact Freezing of Unfixed Tissue; 3. Contact Freezing of Fixed Tissue; 4. High-Pressure Freezing; 5. Freeze-Substitution in Methanol/Uranyl Acetate; 6. Freeze-Substitution in Osmium Tetroxide Acetone; 7. Progressive Lowering of Temperature Embedding in Lowicryl; CHAPTER 7. SUPPORT FILMS; 1. Surface Topography; 2. Stability of Film or Section; 3. Holey Films; 4. Thick and Thin Support Films; 5. Folds in Support Film; 6. Defects in Formvar Films; 7. Common Contaminants; 8. Volatile Contamination; CHAPTER 8. ULTRAMICROTOMY 1. Correlation of Light and Electron Microscopy |
Record Nr. | UNINA-9910457984003321 |
Maunsbach Arvid Bernhard | ||
San Diego, CA, : Academic Press, c1999 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Biomedical electron microscopy [[electronic resource] ] : illustrated methods and interpretations / / Arvid B. Maunsbach, Björn A. Afzelius |
Autore | Maunsbach Arvid Bernhard |
Pubbl/distr/stampa | San Diego, CA, : Academic Press, c1999 |
Descrizione fisica | 1 online resource (569 p.) |
Disciplina |
502.825
570.2825 570/.28/25 21 |
Altri autori (Persone) | AfzeliusBjörn |
Soggetto topico |
Electron microscopy - Technique
Medical microscopy - Technique |
ISBN |
1-281-27948-X
9786611279486 0-08-052809-0 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Front Cover; BIOMEDICAL ELECTRON MICROSCOPY; Copright Page; FOREWORD; PREFACE; ACKNOWLEDGMENTS; CHAPTER 1. MICROGRAPH INTERPRETATION; 1. Classical Preparation Method; 2. Low Temperature Approach; 3. A Common Test Specimen; 4. Detection of Objects; 5. Identification of Artifacts; 6. Analysis of Geometry; 7. Biological Identification; 8. Biological Diversity; 9. Analysis of Dynamics: Endocytosis; 10. Analysis of Dynamics: Synthesis; 11. Comparison of Methods; 12. Variations in Magnifications; 13. Interpretation Difficulties; 14. Diagnostic Pathology; CHAPTER 2. FIXATIVES
1. Osmium Tetroxide and Glutaraldehyde at Low Magnification2. Osmium Tetroxide and Glutaraldehyde at High Magnification; 3. Glutaraldehyde Concentration: Perfusion Fixation; 4. Glutaraldehyde Concentration: Immersion Fixation; 5. Long Fixation Times; 6. Formaldehyde-Glutaraldehyde Combinations; 7. Potassium Permanganate, Picric Acid, and Ruthenium Red; 8. Lead Salts and Tannic Acid; 9. Uranyl Acetate Postfixation; 10. Tannic Acid-Uranyl Acetate Variations; 11. Osmium Tetroxide-Potassium Ferrocyanide; 12. Osmium Tetroxide Artifacts; 13. Glutaraldehyde Artifacts; CHAPTER 3. FIXATIVE VEHICLE 1. Absence and Presence of Buffer2. Comparison of Buffers; 3. Osmolality of Perfusion Fixatives; 4. Effects of Osmolality on Cell Shape; 5. Effects of Osmolality on Cell Organelles; 6. Adjustment of Osmolality with Sucrose; 7. Colloid Osmotic Pressure: Low Magnification; 8. Colloid Osmotic Pressure: High Magnification; 9. Phosphate Buffer Precipitate; CHAPTER 4. FIXATIVE APPLICATION; 1. Perfusion-Fixation versus Immersion-Fixation; 2. Perfusion-Fixation with Pressure Control; 3. Fixation by Dripping in Vivo; 4. Immersion-Fixation; 5. Variability within the Tissue 6. Unsuccessful Perfusion-Fixation7. Superficial Tissue Damage; 8. Early Postmortal Changes; 9. Late Postmortal Changes; 10. Influence of Biopsy Method; 11. Microwave Treatment; CHAPTER 5. DEHYDRATION AND EMBEDDING; 1. Stepwise versus Direct Dehydration; 2. Prolonged Dehydration in Ethanol; 3. Prolonged Dehydration in Acetone; 4. Inert Dehydration; 5. Choice of Intermediate Solvent; 6. Epon, Araldite, and Vestopal: Unstained Sections; 7. Epon, Araldite, and Vestopal: Stained Sections; 8. Different Brands of Epoxy Resins; 9. Spurr and LR White; 10. Embedding of Isolated Cells CHAPTER 6. FREEZING AND LOW-TEMPERATURE EMBEDDING1. Plunge Freezing; 2. Contact Freezing of Unfixed Tissue; 3. Contact Freezing of Fixed Tissue; 4. High-Pressure Freezing; 5. Freeze-Substitution in Methanol/Uranyl Acetate; 6. Freeze-Substitution in Osmium Tetroxide Acetone; 7. Progressive Lowering of Temperature Embedding in Lowicryl; CHAPTER 7. SUPPORT FILMS; 1. Surface Topography; 2. Stability of Film or Section; 3. Holey Films; 4. Thick and Thin Support Films; 5. Folds in Support Film; 6. Defects in Formvar Films; 7. Common Contaminants; 8. Volatile Contamination; CHAPTER 8. ULTRAMICROTOMY 1. Correlation of Light and Electron Microscopy |
Record Nr. | UNINA-9910784528503321 |
Maunsbach Arvid Bernhard | ||
San Diego, CA, : Academic Press, c1999 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|
Biomedical electron microscopy [[electronic resource] ] : illustrated methods and interpretations / / Arvid B. Maunsbach, Björn A. Afzelius |
Autore | Maunsbach Arvid Bernhard |
Edizione | [1st ed.] |
Pubbl/distr/stampa | San Diego, CA, : Academic Press, c1999 |
Descrizione fisica | 1 online resource (569 p.) |
Disciplina |
502.825
570.2825 570/.28/25 21 |
Altri autori (Persone) | AfzeliusBjörn |
Soggetto topico |
Electron microscopy - Technique
Medical microscopy - Technique |
ISBN |
1-281-27948-X
9786611279486 0-08-052809-0 |
Formato | Materiale a stampa |
Livello bibliografico | Monografia |
Lingua di pubblicazione | eng |
Nota di contenuto |
Front Cover; BIOMEDICAL ELECTRON MICROSCOPY; Copright Page; FOREWORD; PREFACE; ACKNOWLEDGMENTS; CHAPTER 1. MICROGRAPH INTERPRETATION; 1. Classical Preparation Method; 2. Low Temperature Approach; 3. A Common Test Specimen; 4. Detection of Objects; 5. Identification of Artifacts; 6. Analysis of Geometry; 7. Biological Identification; 8. Biological Diversity; 9. Analysis of Dynamics: Endocytosis; 10. Analysis of Dynamics: Synthesis; 11. Comparison of Methods; 12. Variations in Magnifications; 13. Interpretation Difficulties; 14. Diagnostic Pathology; CHAPTER 2. FIXATIVES
1. Osmium Tetroxide and Glutaraldehyde at Low Magnification2. Osmium Tetroxide and Glutaraldehyde at High Magnification; 3. Glutaraldehyde Concentration: Perfusion Fixation; 4. Glutaraldehyde Concentration: Immersion Fixation; 5. Long Fixation Times; 6. Formaldehyde-Glutaraldehyde Combinations; 7. Potassium Permanganate, Picric Acid, and Ruthenium Red; 8. Lead Salts and Tannic Acid; 9. Uranyl Acetate Postfixation; 10. Tannic Acid-Uranyl Acetate Variations; 11. Osmium Tetroxide-Potassium Ferrocyanide; 12. Osmium Tetroxide Artifacts; 13. Glutaraldehyde Artifacts; CHAPTER 3. FIXATIVE VEHICLE 1. Absence and Presence of Buffer2. Comparison of Buffers; 3. Osmolality of Perfusion Fixatives; 4. Effects of Osmolality on Cell Shape; 5. Effects of Osmolality on Cell Organelles; 6. Adjustment of Osmolality with Sucrose; 7. Colloid Osmotic Pressure: Low Magnification; 8. Colloid Osmotic Pressure: High Magnification; 9. Phosphate Buffer Precipitate; CHAPTER 4. FIXATIVE APPLICATION; 1. Perfusion-Fixation versus Immersion-Fixation; 2. Perfusion-Fixation with Pressure Control; 3. Fixation by Dripping in Vivo; 4. Immersion-Fixation; 5. Variability within the Tissue 6. Unsuccessful Perfusion-Fixation7. Superficial Tissue Damage; 8. Early Postmortal Changes; 9. Late Postmortal Changes; 10. Influence of Biopsy Method; 11. Microwave Treatment; CHAPTER 5. DEHYDRATION AND EMBEDDING; 1. Stepwise versus Direct Dehydration; 2. Prolonged Dehydration in Ethanol; 3. Prolonged Dehydration in Acetone; 4. Inert Dehydration; 5. Choice of Intermediate Solvent; 6. Epon, Araldite, and Vestopal: Unstained Sections; 7. Epon, Araldite, and Vestopal: Stained Sections; 8. Different Brands of Epoxy Resins; 9. Spurr and LR White; 10. Embedding of Isolated Cells CHAPTER 6. FREEZING AND LOW-TEMPERATURE EMBEDDING1. Plunge Freezing; 2. Contact Freezing of Unfixed Tissue; 3. Contact Freezing of Fixed Tissue; 4. High-Pressure Freezing; 5. Freeze-Substitution in Methanol/Uranyl Acetate; 6. Freeze-Substitution in Osmium Tetroxide Acetone; 7. Progressive Lowering of Temperature Embedding in Lowicryl; CHAPTER 7. SUPPORT FILMS; 1. Surface Topography; 2. Stability of Film or Section; 3. Holey Films; 4. Thick and Thin Support Films; 5. Folds in Support Film; 6. Defects in Formvar Films; 7. Common Contaminants; 8. Volatile Contamination; CHAPTER 8. ULTRAMICROTOMY 1. Correlation of Light and Electron Microscopy |
Record Nr. | UNINA-9910828688003321 |
Maunsbach Arvid Bernhard | ||
San Diego, CA, : Academic Press, c1999 | ||
Materiale a stampa | ||
Lo trovi qui: Univ. Federico II | ||
|