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Fluorescent and luminescent probes for biological activity [[electronic resource] ] : a practical guide to technology for quantitative real-time analysis / / edited by WT Mason
| Fluorescent and luminescent probes for biological activity [[electronic resource] ] : a practical guide to technology for quantitative real-time analysis / / edited by WT Mason |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | San Diego, Calif., : Academic Press, c1999 |
| Descrizione fisica | 1 online resource (697 p.) |
| Disciplina | 570/.28 |
| Altri autori (Persone) | MasonW. T |
| Collana | Biological techniques |
| Soggetto topico |
Fluorescent probes
Fluorescence |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-05700-2
9786611057008 0-08-053177-6 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide to Technology for Quantitative Real-Time Analysis; Copyright Page; Series Preface; Preface; Contributors; Contents; Part I: Introduction to Fluorescence Microscopy; Chapter One. Fluorescence Microscopy; 1.1 Introduction; 1.2 Microscope design; 1.3 Types of illumination; 1.4 Light sources; 1.5 Filters; 1.6 Objectives and eyepieces; References; Part II: Optical Probes and Their Applications; Chapter Two. Introduction to Fluorescent Probes: Properties, History and Applications; 2.1 Introduction
2.2 Nature of fluorescence and properties of fluorescent probes2.3 Historical developments; 2.4 Applications of fluorochromes in histology and microbiology; 2.5 Introduction of acridine orange into cell physiology, cytology and cytochemistry; 2.6 General applications of fluorescent probes; Acknowledgements; References; Chapter Three. Intracellular Ion Indicators; 3.1 Introduction; 3.2 General properties of intracellular ion indicators; 3.3 Examples of intracellular ion indicators; 3.4 Conclusions; Acknowledgements; References Chapter Four. Fluorescent Imaging of Nucleic Acids and Proteins in Gels4.1 Introduction; 4.2 General properties of fluorescent nucleic acid stains; 4.3 Examples of fluorescent nucleic acid gel stains; 4.4 General properties of fluorophore labels used to detect nucleic acids; 4.5 General properties of fluorescent protein gel stains; 4.6 Examples of fluorescent protein gel stains; 4.7 Protein labelling; 4.8 Conclusions; Acknowledgements; References; Part III: Using Optical Probes in Cells - Practicalities, Problems and Pitfalls Chapter Five. Introducing and Calibrating Fluorescent Probes in Cells and Organelles5.1 Introduction; 5.2 General principles of the loading process; 5.3 General principles of the calibration process; 5.4 Putting principles into practice; Acknowledgements; References; Chapter Six. Electroporation: A Method for Introduction of Non-permeable Molecular Probes; 6.1 Introduction; 6.2 Basic concept of electroporation; 6.3 Electric field generation and monitoring; 6.4 Polarization of the outer membrane; 6.5 Electropore formation and resealing; 6.6 Transmembrane transport 6.7 Practical considerations of electroporation6.8 Experimental evidence; 6.9 Summary; Acknowledgements; References; Chapter Seven. Imaging Reality: Understanding Maps of Physiological Cell Signals Measured by Fluorescence Microscopy and Digital Imaging; 7.1 Introduction; 7.2 Generic considerations for the use of fluorescent indicators; 7.3 Optimization of fluorescent light detection and background light correction; 7.4 3-D spatial maps of fluorescent signals; Acknowledgements; References; Chapter Eight. Fluorescent Probes in Practice - Potential Artifacts; 8.1 Introduction 8.2 Photobleaching |
| Record Nr. | UNINA-9910458576203321 |
| San Diego, Calif., : Academic Press, c1999 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Fluorescent and luminescent probes for biological activity [[electronic resource] ] : a practical guide to technology for quantitative real-time analysis / / edited by WT Mason
| Fluorescent and luminescent probes for biological activity [[electronic resource] ] : a practical guide to technology for quantitative real-time analysis / / edited by WT Mason |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | San Diego, Calif., : Academic Press, c1999 |
| Descrizione fisica | 1 online resource (697 p.) |
| Disciplina | 570/.28 |
| Altri autori (Persone) | MasonW. T |
| Collana | Biological techniques |
| Soggetto topico |
Fluorescent probes
Fluorescence |
| ISBN |
1-281-05700-2
9786611057008 0-08-053177-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide to Technology for Quantitative Real-Time Analysis; Copyright Page; Series Preface; Preface; Contributors; Contents; Part I: Introduction to Fluorescence Microscopy; Chapter One. Fluorescence Microscopy; 1.1 Introduction; 1.2 Microscope design; 1.3 Types of illumination; 1.4 Light sources; 1.5 Filters; 1.6 Objectives and eyepieces; References; Part II: Optical Probes and Their Applications; Chapter Two. Introduction to Fluorescent Probes: Properties, History and Applications; 2.1 Introduction
2.2 Nature of fluorescence and properties of fluorescent probes2.3 Historical developments; 2.4 Applications of fluorochromes in histology and microbiology; 2.5 Introduction of acridine orange into cell physiology, cytology and cytochemistry; 2.6 General applications of fluorescent probes; Acknowledgements; References; Chapter Three. Intracellular Ion Indicators; 3.1 Introduction; 3.2 General properties of intracellular ion indicators; 3.3 Examples of intracellular ion indicators; 3.4 Conclusions; Acknowledgements; References Chapter Four. Fluorescent Imaging of Nucleic Acids and Proteins in Gels4.1 Introduction; 4.2 General properties of fluorescent nucleic acid stains; 4.3 Examples of fluorescent nucleic acid gel stains; 4.4 General properties of fluorophore labels used to detect nucleic acids; 4.5 General properties of fluorescent protein gel stains; 4.6 Examples of fluorescent protein gel stains; 4.7 Protein labelling; 4.8 Conclusions; Acknowledgements; References; Part III: Using Optical Probes in Cells - Practicalities, Problems and Pitfalls Chapter Five. Introducing and Calibrating Fluorescent Probes in Cells and Organelles5.1 Introduction; 5.2 General principles of the loading process; 5.3 General principles of the calibration process; 5.4 Putting principles into practice; Acknowledgements; References; Chapter Six. Electroporation: A Method for Introduction of Non-permeable Molecular Probes; 6.1 Introduction; 6.2 Basic concept of electroporation; 6.3 Electric field generation and monitoring; 6.4 Polarization of the outer membrane; 6.5 Electropore formation and resealing; 6.6 Transmembrane transport 6.7 Practical considerations of electroporation6.8 Experimental evidence; 6.9 Summary; Acknowledgements; References; Chapter Seven. Imaging Reality: Understanding Maps of Physiological Cell Signals Measured by Fluorescence Microscopy and Digital Imaging; 7.1 Introduction; 7.2 Generic considerations for the use of fluorescent indicators; 7.3 Optimization of fluorescent light detection and background light correction; 7.4 3-D spatial maps of fluorescent signals; Acknowledgements; References; Chapter Eight. Fluorescent Probes in Practice - Potential Artifacts; 8.1 Introduction 8.2 Photobleaching |
| Record Nr. | UNINA-9910784529303321 |
| San Diego, Calif., : Academic Press, c1999 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Fluorescent and luminescent probes for biological activity : a practical guide to technology for quantitative real-time analysis / / edited by WT Mason
| Fluorescent and luminescent probes for biological activity : a practical guide to technology for quantitative real-time analysis / / edited by WT Mason |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | San Diego, Calif., : Academic Press, c1999 |
| Descrizione fisica | 1 online resource (697 p.) |
| Disciplina | 570/.28 |
| Altri autori (Persone) | MasonW. T |
| Collana | Biological techniques |
| Soggetto topico |
Fluorescent probes
Fluorescence |
| ISBN |
1-281-05700-2
9786611057008 0-08-053177-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide to Technology for Quantitative Real-Time Analysis; Copyright Page; Series Preface; Preface; Contributors; Contents; Part I: Introduction to Fluorescence Microscopy; Chapter One. Fluorescence Microscopy; 1.1 Introduction; 1.2 Microscope design; 1.3 Types of illumination; 1.4 Light sources; 1.5 Filters; 1.6 Objectives and eyepieces; References; Part II: Optical Probes and Their Applications; Chapter Two. Introduction to Fluorescent Probes: Properties, History and Applications; 2.1 Introduction
2.2 Nature of fluorescence and properties of fluorescent probes2.3 Historical developments; 2.4 Applications of fluorochromes in histology and microbiology; 2.5 Introduction of acridine orange into cell physiology, cytology and cytochemistry; 2.6 General applications of fluorescent probes; Acknowledgements; References; Chapter Three. Intracellular Ion Indicators; 3.1 Introduction; 3.2 General properties of intracellular ion indicators; 3.3 Examples of intracellular ion indicators; 3.4 Conclusions; Acknowledgements; References Chapter Four. Fluorescent Imaging of Nucleic Acids and Proteins in Gels4.1 Introduction; 4.2 General properties of fluorescent nucleic acid stains; 4.3 Examples of fluorescent nucleic acid gel stains; 4.4 General properties of fluorophore labels used to detect nucleic acids; 4.5 General properties of fluorescent protein gel stains; 4.6 Examples of fluorescent protein gel stains; 4.7 Protein labelling; 4.8 Conclusions; Acknowledgements; References; Part III: Using Optical Probes in Cells - Practicalities, Problems and Pitfalls Chapter Five. Introducing and Calibrating Fluorescent Probes in Cells and Organelles5.1 Introduction; 5.2 General principles of the loading process; 5.3 General principles of the calibration process; 5.4 Putting principles into practice; Acknowledgements; References; Chapter Six. Electroporation: A Method for Introduction of Non-permeable Molecular Probes; 6.1 Introduction; 6.2 Basic concept of electroporation; 6.3 Electric field generation and monitoring; 6.4 Polarization of the outer membrane; 6.5 Electropore formation and resealing; 6.6 Transmembrane transport 6.7 Practical considerations of electroporation6.8 Experimental evidence; 6.9 Summary; Acknowledgements; References; Chapter Seven. Imaging Reality: Understanding Maps of Physiological Cell Signals Measured by Fluorescence Microscopy and Digital Imaging; 7.1 Introduction; 7.2 Generic considerations for the use of fluorescent indicators; 7.3 Optimization of fluorescent light detection and background light correction; 7.4 3-D spatial maps of fluorescent signals; Acknowledgements; References; Chapter Eight. Fluorescent Probes in Practice - Potential Artifacts; 8.1 Introduction 8.2 Photobleaching |
| Record Nr. | UNINA-9910828815403321 |
| San Diego, Calif., : Academic Press, c1999 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
How science works [[electronic resource] ] : evaluating evidence in biology and medicine / / Stephen H. Jenkins
| How science works [[electronic resource] ] : evaluating evidence in biology and medicine / / Stephen H. Jenkins |
| Autore | Jenkins Stephen H |
| Pubbl/distr/stampa | Oxford ; ; New York, : Oxford University Press, 2004 |
| Descrizione fisica | 1 online resource (x, 227 p. ) : ill |
| Disciplina | 570/.28 |
| Soggetto topico |
Biology - Methodology
Medicine - Methodology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-50291-6
1-282-36743-9 0-19-803604-3 9786612367434 9786610502912 1-60256-706-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910450321403321 |
Jenkins Stephen H
|
||
| Oxford ; ; New York, : Oxford University Press, 2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
How science works [[electronic resource] ] : evaluating evidence in biology and medicine / / Stephen H. Jenkins
| How science works [[electronic resource] ] : evaluating evidence in biology and medicine / / Stephen H. Jenkins |
| Autore | Jenkins Stephen H |
| Pubbl/distr/stampa | Oxford ; ; New York, : Oxford University Press, 2004 |
| Descrizione fisica | 1 online resource (x, 227 p. ) : ill |
| Disciplina | 570/.28 |
| Soggetto topico |
Biology - Methodology
Medicine - Methodology |
| ISBN |
0-19-770133-7
1-280-50291-6 1-282-36743-9 0-19-803604-3 9786612367434 9786610502912 1-60256-706-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910783352903321 |
|
Jenkins Stephen H
|
||
| Oxford ; ; New York, : Oxford University Press, 2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
How science works [[electronic resource] ] : evaluating evidence in biology and medicine / / Stephen H. Jenkins
| How science works [[electronic resource] ] : evaluating evidence in biology and medicine / / Stephen H. Jenkins |
| Autore | Jenkins Stephen H |
| Pubbl/distr/stampa | Oxford ; ; New York, : Oxford University Press, 2004 |
| Descrizione fisica | 1 online resource (x, 227 p. ) : ill |
| Disciplina | 570/.28 |
| Soggetto topico |
Biology - Methodology
Medicine - Methodology |
| ISBN |
0-19-770133-7
1-280-50291-6 1-282-36743-9 0-19-803604-3 9786612367434 9786610502912 1-60256-706-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910813840803321 |
|
Jenkins Stephen H
|
||
| Oxford ; ; New York, : Oxford University Press, 2004 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Infrared and raman spectroscopy of biological materials / edited by Hans-Ulrich Gremlich, Bing Yan
| Infrared and raman spectroscopy of biological materials / edited by Hans-Ulrich Gremlich, Bing Yan |
| Pubbl/distr/stampa | New York : M. Dekker, c2001 |
| Descrizione fisica | ix, 581 p. : ill. (some col.) ; 24 cm |
| Disciplina | 570/.28 |
| Altri autori (Persone) |
Gremlich, Hans-Ulrich
Yan, Bing |
| Collana | Practical spectroscopy ; v. 24 |
| Soggetto topico |
Biology - Technique
Raman spectroscopy Infrared spectroscopy |
| ISBN | 0824704096 (alk. paper) |
| Classificazione |
LC QH324
53.0.64 53.9.2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991002012519707536 |
| New York : M. Dekker, c2001 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
| ||
Molecular imaging [[electronic resource] ] : FRET microscopy and spectroscopy / / edited by Ammasi Periasamy, Richard N. Day
| Molecular imaging [[electronic resource] ] : FRET microscopy and spectroscopy / / edited by Ammasi Periasamy, Richard N. Day |
| Pubbl/distr/stampa | Oxford ; ; New York, : Published for the American Physiological Society by Oxford University Press, 2005 |
| Descrizione fisica | 1 online resource (329 p.) |
| Disciplina | 570/.28 |
| Altri autori (Persone) |
PeriasamyAmmasi
DayRichard N |
| Collana | The American Physiological Society methods in physiology series |
| Soggetto topico |
Fluorescence microscopy
Fluorescence spectroscopy |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-03382-0
9786611033828 0-08-053687-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Imaging: FRET Microscopy and Spectroscopy; Copyright Page; Contents; Contributors; Chapter 1. Proteins and the Flow of Information in Cellular Function; Chapter 2. Basics of Fluorescence and FRET; Chapter 3. An Introduction to Filters and Mirrors for FRET; Chapter 4. FRET Imaging in the Wide-Field Microscope; Chapter 5. Confocal FRET Microscopy: Study of Clustered Distribution of Receptor-Ligand Complexes in Endocytic Membranes; Chapter 6. Multiphoton FRET Microscopy for Protein Localization in Tissue; Chapter 7. FRET Data Analysis: The Algorithm
Chapter 8. Photobleaching FRET MicroscopyChapter 9. Single-Molecule FRET; Chapter 10. FRET Measurements Using Multispectral Imaging; Chapter 11. Real-Time Fluorescence Lifetime Imaging and FRET Using Fast-Gated Image Intensifiers; Chapter 12. Streak Fluorescence Lifetime Imaging Microscopy: A Novel Technology for Quantitative FRET Imaging; Chapter 13. Time-Correlated Single Photon Counting Fluorescence Lifetime Imaging- FRET Microscopy for Protein Localization; Chapter 14. Bioluminescence Resonance Energy Transfer: Techniques and Potential Chapter 15. Quantifying Molecular Interactions with Fluorescence Correlation SpectroscopyChapter 16. Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Index |
| Record Nr. | UNINA-9910458576903321 |
| Oxford ; ; New York, : Published for the American Physiological Society by Oxford University Press, 2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular imaging [[electronic resource] ] : FRET microscopy and spectroscopy / / edited by Ammasi Periasamy, Richard N. Day
| Molecular imaging [[electronic resource] ] : FRET microscopy and spectroscopy / / edited by Ammasi Periasamy, Richard N. Day |
| Pubbl/distr/stampa | Oxford ; ; New York, : Published for the American Physiological Society by Oxford University Press, 2005 |
| Descrizione fisica | 1 online resource (329 p.) |
| Disciplina | 570/.28 |
| Altri autori (Persone) |
PeriasamyAmmasi
DayRichard N |
| Collana | The American Physiological Society methods in physiology series |
| Soggetto topico |
Fluorescence microscopy
Fluorescence spectroscopy |
| ISBN |
1-281-03382-0
9786611033828 0-08-053687-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Imaging: FRET Microscopy and Spectroscopy; Copyright Page; Contents; Contributors; Chapter 1. Proteins and the Flow of Information in Cellular Function; Chapter 2. Basics of Fluorescence and FRET; Chapter 3. An Introduction to Filters and Mirrors for FRET; Chapter 4. FRET Imaging in the Wide-Field Microscope; Chapter 5. Confocal FRET Microscopy: Study of Clustered Distribution of Receptor-Ligand Complexes in Endocytic Membranes; Chapter 6. Multiphoton FRET Microscopy for Protein Localization in Tissue; Chapter 7. FRET Data Analysis: The Algorithm
Chapter 8. Photobleaching FRET MicroscopyChapter 9. Single-Molecule FRET; Chapter 10. FRET Measurements Using Multispectral Imaging; Chapter 11. Real-Time Fluorescence Lifetime Imaging and FRET Using Fast-Gated Image Intensifiers; Chapter 12. Streak Fluorescence Lifetime Imaging Microscopy: A Novel Technology for Quantitative FRET Imaging; Chapter 13. Time-Correlated Single Photon Counting Fluorescence Lifetime Imaging- FRET Microscopy for Protein Localization; Chapter 14. Bioluminescence Resonance Energy Transfer: Techniques and Potential Chapter 15. Quantifying Molecular Interactions with Fluorescence Correlation SpectroscopyChapter 16. Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Index |
| Record Nr. | UNINA-9910784529103321 |
| Oxford ; ; New York, : Published for the American Physiological Society by Oxford University Press, 2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Molecular imaging : FRET microscopy and spectroscopy / / edited by Ammasi Periasamy, Richard N. Day
| Molecular imaging : FRET microscopy and spectroscopy / / edited by Ammasi Periasamy, Richard N. Day |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Oxford ; ; New York, : Published for the American Physiological Society by Oxford University Press, 2005 |
| Descrizione fisica | 1 online resource (329 p.) |
| Disciplina | 570/.28 |
| Altri autori (Persone) |
PeriasamyAmmasi
DayRichard N |
| Collana | The American Physiological Society methods in physiology series |
| Soggetto topico |
Fluorescence microscopy
Fluorescence spectroscopy |
| ISBN |
1-281-03382-0
9786611033828 0-08-053687-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Molecular Imaging: FRET Microscopy and Spectroscopy; Copyright Page; Contents; Contributors; Chapter 1. Proteins and the Flow of Information in Cellular Function; Chapter 2. Basics of Fluorescence and FRET; Chapter 3. An Introduction to Filters and Mirrors for FRET; Chapter 4. FRET Imaging in the Wide-Field Microscope; Chapter 5. Confocal FRET Microscopy: Study of Clustered Distribution of Receptor-Ligand Complexes in Endocytic Membranes; Chapter 6. Multiphoton FRET Microscopy for Protein Localization in Tissue; Chapter 7. FRET Data Analysis: The Algorithm
Chapter 8. Photobleaching FRET MicroscopyChapter 9. Single-Molecule FRET; Chapter 10. FRET Measurements Using Multispectral Imaging; Chapter 11. Real-Time Fluorescence Lifetime Imaging and FRET Using Fast-Gated Image Intensifiers; Chapter 12. Streak Fluorescence Lifetime Imaging Microscopy: A Novel Technology for Quantitative FRET Imaging; Chapter 13. Time-Correlated Single Photon Counting Fluorescence Lifetime Imaging- FRET Microscopy for Protein Localization; Chapter 14. Bioluminescence Resonance Energy Transfer: Techniques and Potential Chapter 15. Quantifying Molecular Interactions with Fluorescence Correlation SpectroscopyChapter 16. Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Index |
| Record Nr. | UNINA-9910817984903321 |
| Oxford ; ; New York, : Published for the American Physiological Society by Oxford University Press, 2005 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||