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A laboratory manual of analytical methods of protein chemistry (including polypeptides) / editors P. Alexander, R.J. Block
| A laboratory manual of analytical methods of protein chemistry (including polypeptides) / editors P. Alexander, R.J. Block |
| Pubbl/distr/stampa | London [etc.], : Pergamon Press |
| Descrizione fisica | 5 volumi : ill. ; 24 cm |
| Disciplina | 547.75046 |
| Soggetto non controllato | Proteine - Analisi - Manuali |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | 1.: The separation and isolation of proteins 2.: The composition, structure and reactivity of proteins 3.: Determination of the size and shape of protein molecules 4.: / eds. P. Alexander, H.P. Lundgren 5.: / eds. P. Alexander, H.P. Lundgren |
| Record Nr. | UNINA-9910853798803321 |
| London [etc.], : Pergamon Press | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Crystallography made crystal clear : a guide for users of macromolecular models / Gale Rhodes
| Crystallography made crystal clear : a guide for users of macromolecular models / Gale Rhodes |
| Autore | Rhodes, Gale |
| Edizione | [2nd. ed.] |
| Pubbl/distr/stampa | San Diego : Academic Press, c2000 |
| Descrizione fisica | xix, 269 p., [16] p. of col. plates : ill. ; 23 cm |
| Disciplina | 547.75046 |
| Soggetto topico |
X-ray crystallography
Macromolecules - Structure Proteins - Structure |
| ISBN | 0125870728 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991001926979707536 |
Rhodes, Gale
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| San Diego : Academic Press, c2000 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
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Microcharacterization of proteins / / R. Kellner, F. Lottspeich, H. E. Meyer
| Microcharacterization of proteins / / R. Kellner, F. Lottspeich, H. E. Meyer |
| Autore | Kellner R. |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Weinheim, Germany : , : WILEY-VCH, , 1999 |
| Descrizione fisica | 1 online resource (349 p.) |
| Disciplina |
547.75046
572.65 |
| Soggetto topico |
Proteins - Analysis - Methodology
Peptides - Analysis - Methodology Amino acids - Analysis - Methodology |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-84250-8
9786611842505 3-527-61396-X 3-527-61397-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Microcharacterization of Proteins; Contents; Section I: Overview; I.1 Microcharacterization of Proteins; 1 General Aspects; 2 From a Cell to a Protein Sequence; 3 Genome and Proteome; Section II: Microseparation Techniques and Sample Preparation; II.1 Microseparation Techniques I: High Resolution Gel-Electrophoretic Techniques : Qualitative, Quantitative and Micropreparative Applications; 1 Introduction; 2 Theory; 3 Media and Equipment; 3.1 Media; 3.2 Equipment; 4 Gel Electrophoretic Methods; 4.1 Disc Electrophoresis; 4.2 Gradient Gel Electrophoresis; 4.3 Additives in Electrophoresis
4.4 SDS Electrophoresis4.5 Isoelectric Focusing; 4.6 Two-Dimensional Electrophoresis (2 -DE); 5 References; II.2 Microseparation Techniques II: Gel Electrophoresis for Sample Preparation in Protein Chemistry; 1 Introduction; 2 Denaturing Techniques; 2.1 Commonly Used SDS-Polyacrylamide Gel Electrophoresis Techniques for Protein Separation; 2.2 Blue-SDS-PAGE for Recovery of Membrane Proteins from Gels; 2.3 Electroelution of Proteins after Blue-SDS-PAGE; 2.4 Electroblotting of Blue and Colorless SDS Gels; 2.5 Isoelectric Focusing in the Presence of Urea; 3 Native Techniques 3.1 Colorless-Native-PAGE3.2 Blue-Native-PAGE; 3.3 Native Isoelectric Focusing; 4 References; II.3 Microseparation Techniques III: Electroblotting; 1 Introduction; 2 Electroblotting; 2.1 Polyacrylamide Gel Electrophoresis; 2.2 Blot Systems; 2.2.1 Tank Blotting; 2.2.2 Semidiy Blotting; 2.3 Blotting Parameters; 2.3.1 The Blotting Process; 2.3.2 Transfer Buffers; 2.3.3 Addition of SDS; 2.3.4 Addition of Methanol; 2.3.5 Influence of Protein Concentration; 3 Blotting Membranes; 4 References; II.4 Microseparation Techniques IV: Analysis of Peptides and Proteins by Capillary Electrophoresis 1 Introduction2 Theory; 2.1 Capillary Isotachophoresis; 2.2 Capillary Zone Electrophoresis; 2.3 Electroosmotic Flow; 3 Instrumentation; 3.1 Injection; 3.2 Detection; 4 Applications; 4.1 Peptide Separations; 4.2 Protein Separations; 5 References; II.5 Microseparation Techniques V: High Performance Liquid Chromatography; 1 Introduction; 2 Principle of HPLC; 3 Getting Started; 3.1 Solvents; 3.2 Pump; 3.3 Gradient; 3.4 Pre-Column Split; 3.5 Sample Preparation; 3.6 Injector; 3.7 Tubings; 3.8 In-Line Filter, Guard Column; 3.9 Column; 3.10 Detection; 3.11 Fractionation; 4 Applications; 5 References II.6 Sample Preparation I: Removal of Salts and Detergents1 Introduction; 2 Removal of Salts or Polar Components; 2.1 Protein-Binding Membranes; 2.2 High Performance Liquid Chromatography; 2.3 Desalting on Microcolumns; 3 Removal of Detergents and Apolar Contaminants; 3.1 Detergents; 3.2 Protein-binding Membranes; 3.3 Precipitation; 3.4 SDS-PAGE; 3.5 Concentrating Gels; 4 "Golden Rules" for Protein and Peptide Handling; 5 References; II.7 Sample Preparation II: Chemical and Enzymatic Fragmentation of Proteins; 1 Strategy; 2 Denaturation, Reduction and Alkylation; 3 Enzymatic Fragmentation 3.1 Enzymes |
| Record Nr. | UNINA-9910144009803321 |
|
Kellner R.
|
||
| Weinheim, Germany : , : WILEY-VCH, , 1999 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Microcharacterization of proteins / / R. Kellner, F. Lottspeich, H. E. Meyer
| Microcharacterization of proteins / / R. Kellner, F. Lottspeich, H. E. Meyer |
| Autore | Kellner R. |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Weinheim, Germany : , : WILEY-VCH, , 1999 |
| Descrizione fisica | 1 online resource (349 p.) |
| Disciplina |
547.75046
572.65 |
| Soggetto topico |
Proteins - Analysis - Methodology
Peptides - Analysis - Methodology Amino acids - Analysis - Methodology |
| ISBN |
1-281-84250-8
9786611842505 3-527-61396-X 3-527-61397-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Microcharacterization of Proteins; Contents; Section I: Overview; I.1 Microcharacterization of Proteins; 1 General Aspects; 2 From a Cell to a Protein Sequence; 3 Genome and Proteome; Section II: Microseparation Techniques and Sample Preparation; II.1 Microseparation Techniques I: High Resolution Gel-Electrophoretic Techniques : Qualitative, Quantitative and Micropreparative Applications; 1 Introduction; 2 Theory; 3 Media and Equipment; 3.1 Media; 3.2 Equipment; 4 Gel Electrophoretic Methods; 4.1 Disc Electrophoresis; 4.2 Gradient Gel Electrophoresis; 4.3 Additives in Electrophoresis
4.4 SDS Electrophoresis4.5 Isoelectric Focusing; 4.6 Two-Dimensional Electrophoresis (2 -DE); 5 References; II.2 Microseparation Techniques II: Gel Electrophoresis for Sample Preparation in Protein Chemistry; 1 Introduction; 2 Denaturing Techniques; 2.1 Commonly Used SDS-Polyacrylamide Gel Electrophoresis Techniques for Protein Separation; 2.2 Blue-SDS-PAGE for Recovery of Membrane Proteins from Gels; 2.3 Electroelution of Proteins after Blue-SDS-PAGE; 2.4 Electroblotting of Blue and Colorless SDS Gels; 2.5 Isoelectric Focusing in the Presence of Urea; 3 Native Techniques 3.1 Colorless-Native-PAGE3.2 Blue-Native-PAGE; 3.3 Native Isoelectric Focusing; 4 References; II.3 Microseparation Techniques III: Electroblotting; 1 Introduction; 2 Electroblotting; 2.1 Polyacrylamide Gel Electrophoresis; 2.2 Blot Systems; 2.2.1 Tank Blotting; 2.2.2 Semidiy Blotting; 2.3 Blotting Parameters; 2.3.1 The Blotting Process; 2.3.2 Transfer Buffers; 2.3.3 Addition of SDS; 2.3.4 Addition of Methanol; 2.3.5 Influence of Protein Concentration; 3 Blotting Membranes; 4 References; II.4 Microseparation Techniques IV: Analysis of Peptides and Proteins by Capillary Electrophoresis 1 Introduction2 Theory; 2.1 Capillary Isotachophoresis; 2.2 Capillary Zone Electrophoresis; 2.3 Electroosmotic Flow; 3 Instrumentation; 3.1 Injection; 3.2 Detection; 4 Applications; 4.1 Peptide Separations; 4.2 Protein Separations; 5 References; II.5 Microseparation Techniques V: High Performance Liquid Chromatography; 1 Introduction; 2 Principle of HPLC; 3 Getting Started; 3.1 Solvents; 3.2 Pump; 3.3 Gradient; 3.4 Pre-Column Split; 3.5 Sample Preparation; 3.6 Injector; 3.7 Tubings; 3.8 In-Line Filter, Guard Column; 3.9 Column; 3.10 Detection; 3.11 Fractionation; 4 Applications; 5 References II.6 Sample Preparation I: Removal of Salts and Detergents1 Introduction; 2 Removal of Salts or Polar Components; 2.1 Protein-Binding Membranes; 2.2 High Performance Liquid Chromatography; 2.3 Desalting on Microcolumns; 3 Removal of Detergents and Apolar Contaminants; 3.1 Detergents; 3.2 Protein-binding Membranes; 3.3 Precipitation; 3.4 SDS-PAGE; 3.5 Concentrating Gels; 4 "Golden Rules" for Protein and Peptide Handling; 5 References; II.7 Sample Preparation II: Chemical and Enzymatic Fragmentation of Proteins; 1 Strategy; 2 Denaturation, Reduction and Alkylation; 3 Enzymatic Fragmentation 3.1 Enzymes |
| Record Nr. | UNINA-9910830665603321 |
|
Kellner R.
|
||
| Weinheim, Germany : , : WILEY-VCH, , 1999 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Microcharacterization of proteins [[electronic resource] /] / R. Kellner, F. Lottspeich, H.E. Meyer
| Microcharacterization of proteins [[electronic resource] /] / R. Kellner, F. Lottspeich, H.E. Meyer |
| Autore | Kellner R (Roland) |
| Pubbl/distr/stampa | Weinheim, : VCH, c1994 |
| Descrizione fisica | 1 online resource (288 p.) |
| Disciplina |
547.75046
572/.65 |
| Altri autori (Persone) |
LottspeichF <1947-> (Friedrich)
MeyerH. E (Helmut E.) |
| Soggetto topico |
Proteins
Proteins - Analysis |
| ISBN |
1-281-75875-2
9786611758752 3-527-61571-7 3-527-61570-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Microcharacterization of Proteins; Contents; Section I: Overview; 1.1 Microcharacterization of Proteins; 1 General Aspects; 2 From a Cell to a Protein Sequence; 3 FutureTrends; Section II: Sample Preparation; II.1 Chemical and Enzymatic Fragmentation of Proteins; 1 Strategy; 2 Denaturation, Reduction and Alkylation; 3 Enzymatic Fragmentation; 3.1 Enzymes; 3.2 Practical Considerations; 4 Chemical Fragmentation; 4.1 Cyanogen Bromide Cleavage; 4.2 Partial Acid Hydrolysis; 4.3 Hydroxylamine Cleavage of Asn-Gly Bonds; 4.4 Cleavage at Tryptophan; 4.5 Cleavage at Cysteine; 5 References
II.2 Microseparation Techniques I: High Performance Liquid Chromatography1 Introduction; 2 Getting Started; 2.1 Solvents; 2.2 Pump; 2.3 Pre-Column Split; 2.4 Sample Preparation; 2.5 Injector; 2.6 Tubings; 2.7 In-Line Filter, Guard Column; 2.8 Analytical Column; 2.9 Elution; 2.10 Detection; 2.11 Fractionation; 3 Applications; 4 References; II.3 Microseparation Techniques II: Analysis of Peptides and Proteins by Capillary Electrophoresis; 1 Introduction; 2 Theory; 2.1 Capillary Isotachophoresis; 2.2 Capillary Zone Electrophoresis; 2.3 Electroosmotic Flow; 3 Instrumentation; 3.1 Injection 3.2 Detection4 Applications; 4.1 Peptide Separations; 4.2 Protein Separations; 5 References; II.4 Microseparation Techniques III: Gel Electrophoresis for Sample Preparation in Protein Chemistry; 1 Introduction; 2 Denaturing Techniques; 2.1Commonly Used SDS-Polyacrylamide Gel Electrophoresis Techniques for Protein Separation; 2.2 Blue-SDS-PAGE for Quantitative Protein Recovery from Gels; 2.3 Electroelution of Proteins After Blue-SDS-PAGE; 2.4 Electroblotting of Blue and Colourless SDS Gels; 2.5 Isoelectric Focusing in the Presence of Urea; 3 Native Techniques; 3.1 Colourless-Native-PAGE 3.2 Blue-Native-PAGE3.3 Native Isoelectric Focusing; 4 References; II.5 Microseparation Techniques IV: Electroblotting; 1 Introduction; 2 Electroblotting; 2.1 Polyacrylamide Gel Electrophoresis; 2.2 Blot Systems; 2.2.1 Tank Blotting; 2.2.2 Semidry Blotting; 2.3 Blotting Parameters; 2.3.1 The Blotting Process; 2.3.2 Transfer Buffers; 2.3.3 Addition of SDS; 2.3.4 Addition of Methanol; 2.3.5 Influence of Protein Concentration; 3 Blotting Membranes; 4 References; Section III: Amino Acid Analysis; III.1 Amino Acid Analysis; 1 Introduction; 2 Sample Preparation; 2.1 Peptides and Proteins 2.1.1 Enzymatic Hydrolysis2.1.2 Acid Hydrolysis; 2.1.3 Alkaline Hydrolysis; 2.2 Free Amino Acids; 3 Derivatization; 3.1 Post-Column Derivatization; 3.1.1 Ninhydrin; 3.1.2 Orthophthaldialdehyde; 3.1.3 Fluorescamine; 3.2 Pre-Column Derivatization; 3.2.1 Phenylisothiocyanate; 3.2.2 Orthophthaldialdehyde; 3.2.3 Fluorenylmethyl Chloroformate; 3.2.4 Dabsyl Chloride; 3.2.5 Dansyl Chloride; 3.2.6 Chiral Reagents; 4 Data Evaluation; 5 Instrumentation; 6 Discussion; 7 References; Section IV. Protein Sequence Analysis; IV. 1 The Edman Degradation; 1 The Edman Chemistry 1.1 Coupling, Cleavage and Conversion |
| Record Nr. | UNISA-996197616503316 |
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Kellner R (Roland)
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| Weinheim, : VCH, c1994 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
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