top

  Info

  • Utilizzare la checkbox di selezione a fianco di ciascun documento per attivare le funzionalità di stampa, invio email, download nei formati disponibili del (i) record.

  Info

  • Utilizzare questo link per rimuovere la selezione effettuata.

Biblioteca

MARC Lista (tabellare)
Handbook of HPLC / / editor, Danilo Corradini
Handbook of HPLC / / editor, Danilo Corradini
Edizione [2nd ed.]
Pubbl/distr/stampa Boca Raton : , : Taylor & Francis, , 2010
Descrizione fisica XIX, 695 p : il ; 26 cm
Disciplina 543/.84
Altri autori (Persone) CorradiniDanilo
Collana Chromatographic science series
Soggetto topico High performance liquid chromatography
Liquid chromatography
Soggetto genere / forma Electronic books.
ISBN 0-429-11858-9
1-4200-1694-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto part PART I Fundamentals -- chapter 1 Monolithic Stationary Phases in HPLC -- chapter 2 Bonded Stationary Phases -- chapter 3 Micro-HPLC -- chapter 4 Two-DimensionalComprehensive Liquid Chromatography -- chapter 5 Gradient Elution Mode -- chapter 6 Capillary Electromigration Techniques -- chapter 7 HPLC Detectors -- chapter 8 LC-MS Interfaces: State of theArt and Emerging Techniques -- chapter 9 Control and Effectsof Temperature in Analytical HPLC -- chapter 10 Nonlinear Liquid Chromatography -- chapter 11 Displacement Chromatographyin the Separation andCharacterization ofProteins and Peptides -- chapter 12 Field-Flow Fractionation -- chapter 13 Affinity Chromatography -- chapter 14 Ion Chromatography: Modesfor Metal Ions Analysis -- chapter 15 Retention Modelsfor Ions in HPLC -- chapter 16 Polymer HPLC -- part PART II Applications -- chapter 17 HPLC in ChiralPharmaceutical Analysis -- chapter 18 HPLC in Environmental Analysis -- chapter 19 HPLC in Food Analysis -- chapter 20 HPLC in Forensic Sciences.
Record Nr. UNINA-9910458858003321
Boca Raton : , : Taylor & Francis, , 2010
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Handbook of HPLC / / editor, Danilo Corradini
Handbook of HPLC / / editor, Danilo Corradini
Edizione [2nd ed.]
Pubbl/distr/stampa Boca Raton : , : Taylor & Francis, , 2010
Descrizione fisica XIX, 695 p : il ; 26 cm
Disciplina 543/.84
Altri autori (Persone) CorradiniDanilo
Collana Chromatographic science series
Soggetto topico High performance liquid chromatography
Liquid chromatography
ISBN 0-429-11858-9
1-4200-1694-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto part PART I Fundamentals -- chapter 1 Monolithic Stationary Phases in HPLC -- chapter 2 Bonded Stationary Phases -- chapter 3 Micro-HPLC -- chapter 4 Two-DimensionalComprehensive Liquid Chromatography -- chapter 5 Gradient Elution Mode -- chapter 6 Capillary Electromigration Techniques -- chapter 7 HPLC Detectors -- chapter 8 LC-MS Interfaces: State of theArt and Emerging Techniques -- chapter 9 Control and Effectsof Temperature in Analytical HPLC -- chapter 10 Nonlinear Liquid Chromatography -- chapter 11 Displacement Chromatographyin the Separation andCharacterization ofProteins and Peptides -- chapter 12 Field-Flow Fractionation -- chapter 13 Affinity Chromatography -- chapter 14 Ion Chromatography: Modesfor Metal Ions Analysis -- chapter 15 Retention Modelsfor Ions in HPLC -- chapter 16 Polymer HPLC -- part PART II Applications -- chapter 17 HPLC in ChiralPharmaceutical Analysis -- chapter 18 HPLC in Environmental Analysis -- chapter 19 HPLC in Food Analysis -- chapter 20 HPLC in Forensic Sciences.
Record Nr. UNINA-9910785026303321
Boca Raton : , : Taylor & Francis, , 2010
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Handbook of HPLC / / editor, Danilo Corradini
Handbook of HPLC / / editor, Danilo Corradini
Edizione [2nd ed.]
Pubbl/distr/stampa Boca Raton : , : Taylor & Francis, , 2010
Descrizione fisica XIX, 695 p : il ; 26 cm
Disciplina 543/.84
Altri autori (Persone) CorradiniDanilo
Collana Chromatographic science series
Soggetto topico High performance liquid chromatography
Liquid chromatography
ISBN 0-429-11858-9
1-4200-1694-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto part PART I Fundamentals -- chapter 1 Monolithic Stationary Phases in HPLC -- chapter 2 Bonded Stationary Phases -- chapter 3 Micro-HPLC -- chapter 4 Two-DimensionalComprehensive Liquid Chromatography -- chapter 5 Gradient Elution Mode -- chapter 6 Capillary Electromigration Techniques -- chapter 7 HPLC Detectors -- chapter 8 LC-MS Interfaces: State of theArt and Emerging Techniques -- chapter 9 Control and Effectsof Temperature in Analytical HPLC -- chapter 10 Nonlinear Liquid Chromatography -- chapter 11 Displacement Chromatographyin the Separation andCharacterization ofProteins and Peptides -- chapter 12 Field-Flow Fractionation -- chapter 13 Affinity Chromatography -- chapter 14 Ion Chromatography: Modesfor Metal Ions Analysis -- chapter 15 Retention Modelsfor Ions in HPLC -- chapter 16 Polymer HPLC -- part PART II Applications -- chapter 17 HPLC in ChiralPharmaceutical Analysis -- chapter 18 HPLC in Environmental Analysis -- chapter 19 HPLC in Food Analysis -- chapter 20 HPLC in Forensic Sciences.
Record Nr. UNINA-9910799965603321
Boca Raton : , : Taylor & Francis, , 2010
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Handbook of HPLC / / editor, Danilo Corradini
Handbook of HPLC / / editor, Danilo Corradini
Edizione [2nd ed.]
Pubbl/distr/stampa Boca Raton, FL, : Taylor & Francis, 2010
Descrizione fisica XIX, 695 p : il ; 26 cm
Disciplina 543/.84
Altri autori (Persone) CorradiniDanilo
Collana Chromatographic science series
Soggetto topico High performance liquid chromatography
Liquid chromatography
ISBN 0-429-11858-9
1-4200-1694-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto part PART I Fundamentals -- chapter 1 Monolithic Stationary Phases in HPLC -- chapter 2 Bonded Stationary Phases -- chapter 3 Micro-HPLC -- chapter 4 Two-DimensionalComprehensive Liquid Chromatography -- chapter 5 Gradient Elution Mode -- chapter 6 Capillary Electromigration Techniques -- chapter 7 HPLC Detectors -- chapter 8 LC-MS Interfaces: State of theArt and Emerging Techniques -- chapter 9 Control and Effectsof Temperature in Analytical HPLC -- chapter 10 Nonlinear Liquid Chromatography -- chapter 11 Displacement Chromatographyin the Separation andCharacterization ofProteins and Peptides -- chapter 12 Field-Flow Fractionation -- chapter 13 Affinity Chromatography -- chapter 14 Ion Chromatography: Modesfor Metal Ions Analysis -- chapter 15 Retention Modelsfor Ions in HPLC -- chapter 16 Polymer HPLC -- part PART II Applications -- chapter 17 HPLC in ChiralPharmaceutical Analysis -- chapter 18 HPLC in Environmental Analysis -- chapter 19 HPLC in Food Analysis -- chapter 20 HPLC in Forensic Sciences.
Record Nr. UNINA-9910825469803321
Boca Raton, FL, : Taylor & Francis, 2010
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
High-performance gradient elution : the practical application of the linear-solvent-strength model / / Lloyd R. Snyder, John W. Dolan
High-performance gradient elution : the practical application of the linear-solvent-strength model / / Lloyd R. Snyder, John W. Dolan
Autore Snyder Lloyd R
Pubbl/distr/stampa Hoboken, NJ, : John Wiley, c2007
Descrizione fisica 1 online resource (491 p.)
Disciplina 543/.84
Altri autori (Persone) DolanJohn W
Soggetto topico High performance liquid chromatography
ISBN 1-280-72143-X
9786610721436
0-470-05552-9
0-470-05551-0
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto HIGH-PERFORMANCE GRADIENT ELUTION; CONTENTS; PREFACE; GLOSSARY OF SYMBOLS AND TERMS; 1 INTRODUCTION; 1.1 The "General Elution Problem" and the Need for Gradient Elution; 1.2 Other Reasons for the Use of Gradient Elution; 1.3 Gradient Shape; 1.4 Similarity of Isocratic and Gradient Elution; 1.4.1 Gradient and Isocratic Elution Compared; 1.4.2 The Linear-Solvent-Strength Model; 1.5 Computer Simulation; 1.6 Sample Classification; 1.6.1 Sample Compounds of Related Structure ("Regular Samples"); 1.6.2 Sample Compounds of Unrelated Structure ("Irregular" Samples); 2 GRADIENT ELUTION FUNDAMENTALS
2.1 Isocratic Separation2.1.1 Retention; 2.1.2 Peak Width and Plate Number; 2.1.3 Resolution; 2.1.4 Role of Separation Conditions; 2.1.4.1 Optimizing Retention [Term a of Equation (2.7)]; 2.1.4.2 Optimizing Selectivity α [Term b of Equation (2.7)]; 2.1.4.3 Optimizing the Column Plate Number N [Term c of Equation (2.7)]; 2.2 Gradient Separation; 2.2.1 Retention; 2.2.1.1 Gradient and Isocratic Separation Compared for "Corresponding" Conditions; 2.2.2 Peak Width; 2.2.3 Resolution; 2.2.3.1 Resolution as a Function of Values of S for Two Adjacent Peaks ("Irregular" Samples)
2.2.3.2 Using Gradient Elution to Predict Isocratic Separation2.2.4 Sample Complexity and Peak Capacity; 2.3 Effect of Gradient Conditions on Separation; 2.3.1 Gradient Steepness b: Change in Gradient Time; 2.3.2 Gradient Steepness b: Change in Column Length or Diameter; 2.3.3 Gradient Steepness b: Change in Flow Rate; 2.3.4 Gradient Range Δø: Change in Initial Percentage B (ø(0)); 2.3.5 Gradient Range Δø: Change in Final %B (ø(f)); 2.3.6 Effect of a Gradient Delay; 2.3.6.1 Equipment Dwell Volume; 2.3.7 Effect of Gradient Shape (Nonlinear Gradients)
2.3.8 Overview of the Effect of Gradient Conditions on the Chromatogram2.4 Related Topics; 2.4.1 Nonideal Retention in Gradient Elution; 2.4.2 Gradient Elution Misconceptions; 3 METHOD DEVELOPMENT; 3.1 A Systematic Approach to Method Development; 3.1.1 Separation Goals (Step 1 of Fig. 3.1); 3.1.2 Nature of the Sample (Step 2 of Fig. 3.1); 3.1.3 Initial Experimental Conditions; 3.1.4 Repeatable Results; 3.1.5 Computer Simulation: Yes or No?; 3.1.6 Sample Preparation (Pretreatment); 3.2 Initial Experiments; 3.2.1 Interpreting the Initial Chromatogram (Step 3 of Fig. 3.1)
3.2.1.1 "Trimming" a Gradient Chromatogram3.2.1.2 Possible Problems; 3.3 Developing a Gradient Separation: Resolution versus Conditions; 3.3.1 Optimizing Gradient Retention k* (Step 4 of Fig. 3.1); 3.3.2 Optimizing Gradient Selectivity α* (Step 5 of Fig. 3.1); 3.3.3 Optimizing the Gradient Range (Step 6 of Fig. 3.1); 3.3.3.1 Changes in Selectivity as a Result of Change in k*; 3.3.4 Segmented (Nonlinear) Gradients (Step 6 of Fig. 3.1 Continued); 3.3.5 Optimizing the Column Plate Number N* (Step 7 of Fig. 3.1); 3.3.6 Column Equilibration Between Successive Sample Injections
3.3.7 Fast Separations
Record Nr. UNINA-9910143567103321
Snyder Lloyd R  
Hoboken, NJ, : John Wiley, c2007
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
HPLC, a practical user's guide [[electronic resource] /] / Marvin C. McMaster
HPLC, a practical user's guide [[electronic resource] /] / Marvin C. McMaster
Autore McMaster Marvin C
Edizione [2nd ed.]
Pubbl/distr/stampa Hoboken, N.J., : Wiley-Interscience, c2007
Descrizione fisica 1 online resource (254 p.)
Disciplina 543.84
543/.84
615.1901
Soggetto topico High performance liquid chromatography
ISBN 1-280-72157-X
9786610721573
0-470-07908-8
0-470-07909-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto HPLC; CONTENTS; PREFACE; I HPLC PRIMER; 1 Advantages and Disadvantages of HPLC; 1.1 How It Works; 1.1.1 A Separation Model of the Column; 1.1.2 Basic Hardware: A Quick, First Look; 1.1.3 Use of Solvent Gradients; 1.1.4 Ranges of Compounds; 1.2 Other Ways to Make My Separation; 1.2.1 FPLC-Fast Protein Liquid Chromatography; 1.2.2 LC-Traditional Liquid Chromatography; 1.2.3 GLC-Gas Liquid Chromatography; 1.2.4 SFC-Supercritical Fluid Chromatography; 1.2.5 TLC-Thin Layer Chromatography; 1.2.6 EP-Electrophoresis; 1.2.7 CZE-Capillary Zone Electrophoresis; 2 Selecting an HPLC System
2.1 Characteristic Systems2.1.1 Finding a Fit: Detectors and Data Processing; 2.1.2 System Models: Gradient Versus Isocratic; 2.1.3 Vendor Selection; 2.1.4 Brand Names and Clones; 2.1.5 Hardware-Service-Support; 2.2 System Cost Estimates; 2.2.1 Type I System-QC Isocratic (Cost: 10-15,000); 2.2.2 Type II System-Research Gradient (Cost: 20-25,000); 2.2.3 Type III System-Automated Clinical (Cost: 25-35,000); 2.2.4 Type IV System-Automated Methods (Cost: 30-50,000); 2.3 Columns; 2.3.1 Sizes: Analytical and Preparative; 2.3.2 Separating Modes: Selecting Only What You Need
2.3.3 Tips on Column Use3 Running Your Chromatograph; 3.1 Set-up and Start-up; 3.1.1 Hardware Plumbing 101: Tubing and Fittings; 3.1.2 Connecting Components; 3.1.3 Solvent Clean-up; 3.1.4 Water Purity Test; 3.1.5 Start-up System Flushing; 3.1.6 Column Preparation and Equilibration; 3.2 Sample Preparation and Column Calibration; 3.2.1 Sample Clean-up; 3.2.2 Plate Counts; 3.3 Your First Chromatogram; 3.3.1 Reproducible Injection Techniques; 3.3.2 Simple Scouting for a Mobile Phase; 3.3.3 Examining the Chromatogram; 3.3.4 Basic Calculations of Results; II HPLC OPTIMIZATION; 4 Separation Models
4.1 Partition4.1.1 Separation Parameters; 4.1.2 Efficiency Factor; 4.1.3 Separation (Chemistry) Factor; 4.2 Ion Exchange Chromatography; 4.3 Size Exclusion Chromatography; 4.4 Affinity Chromatography; 5 Column Preparation; 5.1 Column Variations; 5.2 Packing Materials and Hardware; 5.3 Column Selection; 6 Column Aging, Diagnosis, and Healing; 6.1 Packing Degrading-Bonded-Phase Loss; 6.2 Dissolved Packing Material-End Voids; 6.3 Bound Material; 6.4 Pressure Increases; 6.5 Column Channeling-Center-Voids; 6.6 Normal Phase, Ion Exchange, and Size Columns; 6.7 Zirconium and Polymer Columns
7 Partition Chromatography Modifications7.1 Reverse-Phase and Hybrid Silica; 7.1.1 Ionization Suppression; 7.1.2 Ion Pairing; 7.1.3 Organic Modifiers; 7.1.4 Chelation; 7.2 Acidic Phase Silica; 7.3 Reverse-Phase Zirconium; 7.4 Partition Mode Selection; 8 "Nonpartition" Chromatography; 8.1 Ion Exchange; 8.1.1 Cationic:Weak and Strong; 8.1.2 Anionic:Weak and Strong; 8.2 Size Exclusion; 8.2.1 Organic Soluble Samples; 8.2.2 Hydrophilic Protein Separation; 8.3 Affinity Chromatography; 8.3.1 Column Packing Modification; 8.3.2 Chelation and Optically Active Columns; 9 Hardware Specifics
9.1 System Protection
Record Nr. UNINA-9910141139203321
McMaster Marvin C  
Hoboken, N.J., : Wiley-Interscience, c2007
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
HPLC, a practical user's guide [[electronic resource] /] / Marvin C. McMaster
HPLC, a practical user's guide [[electronic resource] /] / Marvin C. McMaster
Autore McMaster Marvin C
Edizione [2nd ed.]
Pubbl/distr/stampa Hoboken, N.J., : Wiley-Interscience, c2007
Descrizione fisica 1 online resource (254 p.)
Disciplina 543.84
543/.84
615.1901
Soggetto topico High performance liquid chromatography
ISBN 1-280-72157-X
9786610721573
0-470-07908-8
0-470-07909-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto HPLC; CONTENTS; PREFACE; I HPLC PRIMER; 1 Advantages and Disadvantages of HPLC; 1.1 How It Works; 1.1.1 A Separation Model of the Column; 1.1.2 Basic Hardware: A Quick, First Look; 1.1.3 Use of Solvent Gradients; 1.1.4 Ranges of Compounds; 1.2 Other Ways to Make My Separation; 1.2.1 FPLC-Fast Protein Liquid Chromatography; 1.2.2 LC-Traditional Liquid Chromatography; 1.2.3 GLC-Gas Liquid Chromatography; 1.2.4 SFC-Supercritical Fluid Chromatography; 1.2.5 TLC-Thin Layer Chromatography; 1.2.6 EP-Electrophoresis; 1.2.7 CZE-Capillary Zone Electrophoresis; 2 Selecting an HPLC System
2.1 Characteristic Systems2.1.1 Finding a Fit: Detectors and Data Processing; 2.1.2 System Models: Gradient Versus Isocratic; 2.1.3 Vendor Selection; 2.1.4 Brand Names and Clones; 2.1.5 Hardware-Service-Support; 2.2 System Cost Estimates; 2.2.1 Type I System-QC Isocratic (Cost: 10-15,000); 2.2.2 Type II System-Research Gradient (Cost: 20-25,000); 2.2.3 Type III System-Automated Clinical (Cost: 25-35,000); 2.2.4 Type IV System-Automated Methods (Cost: 30-50,000); 2.3 Columns; 2.3.1 Sizes: Analytical and Preparative; 2.3.2 Separating Modes: Selecting Only What You Need
2.3.3 Tips on Column Use3 Running Your Chromatograph; 3.1 Set-up and Start-up; 3.1.1 Hardware Plumbing 101: Tubing and Fittings; 3.1.2 Connecting Components; 3.1.3 Solvent Clean-up; 3.1.4 Water Purity Test; 3.1.5 Start-up System Flushing; 3.1.6 Column Preparation and Equilibration; 3.2 Sample Preparation and Column Calibration; 3.2.1 Sample Clean-up; 3.2.2 Plate Counts; 3.3 Your First Chromatogram; 3.3.1 Reproducible Injection Techniques; 3.3.2 Simple Scouting for a Mobile Phase; 3.3.3 Examining the Chromatogram; 3.3.4 Basic Calculations of Results; II HPLC OPTIMIZATION; 4 Separation Models
4.1 Partition4.1.1 Separation Parameters; 4.1.2 Efficiency Factor; 4.1.3 Separation (Chemistry) Factor; 4.2 Ion Exchange Chromatography; 4.3 Size Exclusion Chromatography; 4.4 Affinity Chromatography; 5 Column Preparation; 5.1 Column Variations; 5.2 Packing Materials and Hardware; 5.3 Column Selection; 6 Column Aging, Diagnosis, and Healing; 6.1 Packing Degrading-Bonded-Phase Loss; 6.2 Dissolved Packing Material-End Voids; 6.3 Bound Material; 6.4 Pressure Increases; 6.5 Column Channeling-Center-Voids; 6.6 Normal Phase, Ion Exchange, and Size Columns; 6.7 Zirconium and Polymer Columns
7 Partition Chromatography Modifications7.1 Reverse-Phase and Hybrid Silica; 7.1.1 Ionization Suppression; 7.1.2 Ion Pairing; 7.1.3 Organic Modifiers; 7.1.4 Chelation; 7.2 Acidic Phase Silica; 7.3 Reverse-Phase Zirconium; 7.4 Partition Mode Selection; 8 "Nonpartition" Chromatography; 8.1 Ion Exchange; 8.1.1 Cationic:Weak and Strong; 8.1.2 Anionic:Weak and Strong; 8.2 Size Exclusion; 8.2.1 Organic Soluble Samples; 8.2.2 Hydrophilic Protein Separation; 8.3 Affinity Chromatography; 8.3.1 Column Packing Modification; 8.3.2 Chelation and Optically Active Columns; 9 Hardware Specifics
9.1 System Protection
Record Nr. UNINA-9910830870903321
McMaster Marvin C  
Hoboken, N.J., : Wiley-Interscience, c2007
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
HPLC, a practical user's guide / / Marvin C. McMaster
HPLC, a practical user's guide / / Marvin C. McMaster
Autore McMaster Marvin C
Edizione [2nd ed.]
Pubbl/distr/stampa Hoboken, N.J., : Wiley-Interscience, c2007
Descrizione fisica 1 online resource (254 p.)
Disciplina 543/.84
Soggetto topico High performance liquid chromatography
ISBN 1-280-72157-X
9786610721573
0-470-07908-8
0-470-07909-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto HPLC; CONTENTS; PREFACE; I HPLC PRIMER; 1 Advantages and Disadvantages of HPLC; 1.1 How It Works; 1.1.1 A Separation Model of the Column; 1.1.2 Basic Hardware: A Quick, First Look; 1.1.3 Use of Solvent Gradients; 1.1.4 Ranges of Compounds; 1.2 Other Ways to Make My Separation; 1.2.1 FPLC-Fast Protein Liquid Chromatography; 1.2.2 LC-Traditional Liquid Chromatography; 1.2.3 GLC-Gas Liquid Chromatography; 1.2.4 SFC-Supercritical Fluid Chromatography; 1.2.5 TLC-Thin Layer Chromatography; 1.2.6 EP-Electrophoresis; 1.2.7 CZE-Capillary Zone Electrophoresis; 2 Selecting an HPLC System
2.1 Characteristic Systems2.1.1 Finding a Fit: Detectors and Data Processing; 2.1.2 System Models: Gradient Versus Isocratic; 2.1.3 Vendor Selection; 2.1.4 Brand Names and Clones; 2.1.5 Hardware-Service-Support; 2.2 System Cost Estimates; 2.2.1 Type I System-QC Isocratic (Cost: 10-15,000); 2.2.2 Type II System-Research Gradient (Cost: 20-25,000); 2.2.3 Type III System-Automated Clinical (Cost: 25-35,000); 2.2.4 Type IV System-Automated Methods (Cost: 30-50,000); 2.3 Columns; 2.3.1 Sizes: Analytical and Preparative; 2.3.2 Separating Modes: Selecting Only What You Need
2.3.3 Tips on Column Use3 Running Your Chromatograph; 3.1 Set-up and Start-up; 3.1.1 Hardware Plumbing 101: Tubing and Fittings; 3.1.2 Connecting Components; 3.1.3 Solvent Clean-up; 3.1.4 Water Purity Test; 3.1.5 Start-up System Flushing; 3.1.6 Column Preparation and Equilibration; 3.2 Sample Preparation and Column Calibration; 3.2.1 Sample Clean-up; 3.2.2 Plate Counts; 3.3 Your First Chromatogram; 3.3.1 Reproducible Injection Techniques; 3.3.2 Simple Scouting for a Mobile Phase; 3.3.3 Examining the Chromatogram; 3.3.4 Basic Calculations of Results; II HPLC OPTIMIZATION; 4 Separation Models
4.1 Partition4.1.1 Separation Parameters; 4.1.2 Efficiency Factor; 4.1.3 Separation (Chemistry) Factor; 4.2 Ion Exchange Chromatography; 4.3 Size Exclusion Chromatography; 4.4 Affinity Chromatography; 5 Column Preparation; 5.1 Column Variations; 5.2 Packing Materials and Hardware; 5.3 Column Selection; 6 Column Aging, Diagnosis, and Healing; 6.1 Packing Degrading-Bonded-Phase Loss; 6.2 Dissolved Packing Material-End Voids; 6.3 Bound Material; 6.4 Pressure Increases; 6.5 Column Channeling-Center-Voids; 6.6 Normal Phase, Ion Exchange, and Size Columns; 6.7 Zirconium and Polymer Columns
7 Partition Chromatography Modifications7.1 Reverse-Phase and Hybrid Silica; 7.1.1 Ionization Suppression; 7.1.2 Ion Pairing; 7.1.3 Organic Modifiers; 7.1.4 Chelation; 7.2 Acidic Phase Silica; 7.3 Reverse-Phase Zirconium; 7.4 Partition Mode Selection; 8 "Nonpartition" Chromatography; 8.1 Ion Exchange; 8.1.1 Cationic:Weak and Strong; 8.1.2 Anionic:Weak and Strong; 8.2 Size Exclusion; 8.2.1 Organic Soluble Samples; 8.2.2 Hydrophilic Protein Separation; 8.3 Affinity Chromatography; 8.3.1 Column Packing Modification; 8.3.2 Chelation and Optically Active Columns; 9 Hardware Specifics
9.1 System Protection
Record Nr. UNINA-9910877504003321
McMaster Marvin C  
Hoboken, N.J., : Wiley-Interscience, c2007
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Hydrophilic interaction chromatography [[electronic resource] ] : a guide for practitioners / / edited by Bernard A. Olsen, Brian W. Pack
Hydrophilic interaction chromatography [[electronic resource] ] : a guide for practitioners / / edited by Bernard A. Olsen, Brian W. Pack
Pubbl/distr/stampa Hoboken, N.J., : John Wiley & Sons, Inc., 2013
Descrizione fisica 1 online resource (337 p.)
Disciplina 543/.84
Altri autori (Persone) OlsenBernard A. <1953->
PackBrian W. <1970->
Collana Chemical analysis : a series of monographs on analytical chemistry and its applications
Soggetto topico Hydrophilic interaction liquid chromatography
ISBN 1-118-49524-1
1-283-97811-3
1-118-49521-7
1-118-49523-3
Classificazione SCI013010
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Machine generated contents note: Chapter 1. Separation Mechanisms in Hydrophilic Interaction Chromatography 1.1 Introduction 1.2 Historical Background. Recognition of the contribution of partition, ion exchange and reversed-phase interactions to the retention process 1.3 Recent studies on the contributory mechanisms to HILIC retention 1.3.1 Overview 1.3.2 Contribution of adsorption and partition to HILIC separations 1.3.3 Further studies on the contribution of ionic retention in HILIC 1.3.3.1 Introduction 1.3.3.2 Mobile phase considerations for the separation of ionogenic compounds 1.3.3.3. Ionisation state of the column as a function of pH 1.3.3.4 Quantitation of ionic retention effects on different columns 1.3.4 Reversed-phase retention on bare silica 1.3.5 Electrostatic Repulsion Hydrophilic Interaction Chromatography (ERLIC)- a new separation mode in HILIC. 1.4 Conclusions Chapter 2. Stationary Phases for HILIC 2.1 Introduction 2.2 HILIC stationary phases 2.2.1 Underivatized silica 2.2.1.1 Totally porous silica particles 2.2.1.2 Superficially porous (core shell) silica particles 2.2.1.3 Monolithic silica 2.2.1.4 Ethylene Bridged Hybrids (BEH) 2.2.2 Derivatized silica 2.2.2.1 Neutral derivatized silica 2.2.2.2 Zwitterionic derivatized silica 2.2.2.3 Positively charged derivatized silica 2.2.2.4 Negatively charged derivatized silica 2.2.3 Non-silica phases 2.2.3.1 Amino phases 2.2.3.2 Sulfonated S-DVB phases 2.3 Commercial HILIC phases 2.3.1 Efficiency comparison 2.3.2 Retention and selectivity comparisons 2.4 Conclusions Chapter 3. HILIC Method Development 3.1 Introduction 3.2 General method development considerations 3.2.1 Method objectives 3.2.2 Sample consideration 3.2.3 Systematic method development 3.3 Method development strategies 3.3.1 Systematic approach to column screening 3.3.2 Optimization of method parameters 3.3.2.1 Final column selection 3.3.2.2 Organic solvents 3.3.2.3 Mobile phase pH 3.3.2.4 Buffer types and concentration 3.3.2.5 Column temperature 3.3.2.6 Sample solvents 3.4 Detection for HILIC methods 3.4.1 Mass Spectrometry detector (MS) 3.4.2 Charged aerosol detector (CAD) 3.5 Conclusions Chapter 4. Pharmaceutical Applications of Hydrophilic Interaction Chromatography 4.1 Introduction 4.1.1 Definition of the problem 4.1.2 Selection of conditions 4.1.3 Validation of the method 4.1.4 General references 4.2 Determination of Counterions 4.2.1 Salt selection and options for counterion determination 4.2.2 Specific counterion analysis 4.2.3 Counterion screening with gradient elution 4.2.4 Suitable reference standards for counterion analysis 4.3 Main Component Methods 4.3.1 Potency/assay methods 4.3.2 Equipment cleaning verification assays 4.3.3 Dissolution methods 4.4 Determination of Impurities 4.4.1 Impurity screening and orthogonal separations 4.4.2 Impurity identification 4.4.3 Specific impurity determination 4.4.3.1 Pyrimidines, purines, nucleosides 4.4.3.2 Hydrazines with ethanol as weak solvent 4.4.3.3 Neutral and charged polar impurities in a drug substance 4.4.3.4 Polar basic compounds and impurities 4.4.4 Statistical design of experiments (DOE) for optimization 4.5 Excipients 4.5.1 Parenteral and solution formulations 4.5.2 Tablets, capsules and inhalation products 4.5.3 Sugars 4.5.4 Stabilizers and antioxidants 4.6 Chiral Applications 4.6.1 Chiral selectors and HILIC 4.6.1.1 Cyclodextrins 4.6.1.2 Macrocyclic antibiotics 4.6.1.3 Chiral crown ethers 4.6.1.4 Cyclofructans 4.6.2 Conclusions for chiral separations 4.7 Conclusions Chapter 5. Hydrophilic Interaction Chromatography (HILIC) for Drug Discovery 5.1 Drug Discovery Model 5.2 HILIC Applications for in vitro Biology 5.2.1 Biological screening and hit finding 5.2.1.1 Target selection and assay validation 5.2.1.2 High-throughput screening 5.2.2 New drug discovery strategies 5.3 HILIC Applications and Advances for Discovery Chemistry 5.3.1 Lead identification 5.3.2 Lead optimization 5.3.2.1 ADME profile 5.3.2.2 Biopharmaceutics 5.3.2.3 Chiral purity 5.3.3 Candidate selection 5.4 Practical Considerations 5.5 Conclusions Chapter 6. Advances in Hydrophilic Interaction Chromatography (HILIC) for Biochemical Applications 6.1 Introduction 6.2 Carbohydrates 6.2.1 Mono- and disaccharides 6.2.2 Oligosaccharides and polysaccharides 6.2.3 Glycans 6.2.3.1 Glycan and glycopeptide analysis 6.2.3.2 HILIC for sample enrichment 6.3 Nucleobases and Nucleosides 6.4 Oligonucleotides 6.5 Amino Acids and Peptides 6.6 Proteins 6.7 Phospholipids 6.8 Conclusions Chapter 7. HILIC-MS for Targeted Metabolomics and Small Molecule Bioanalysis 7.1 Introduction 7.2 The role of HILIC-MS in targeted metabolomics versus other LC modes 7.3 Strategies for method development based on retention behavior of targeted metabolites on HILIC stationary phases 7.3.1 Retention behavior of metabolites on HILIC stationary phases 7.3.2 Robustness, mobile phase compositions, and matrix effects 7.4 Summary Chapter 8. HILIC for Food, Environmental, and Other Applications 8.1 Introduction 8.2 Food applications for HILIC 8.2.1 Review of HILIC analytical methods for food analysis 8.2.1.1 Sample preparation in HILIC methods applied to food matrices 8.2.1.2 HILIC methods applied to food matrices: chromatographic parameters and detection 8.2.2 Selected detailed examples of HILIC applications in food analysis 8.2.2.1 Melamine (MEL) and cyanuric acid (CYA) 8.2.2.2 Water soluble vitamins 8.2.2.3 Seafood and other toxins 8.3 Environmental and other applications of HILIC 8.3.1 Review of environmental applications based on the stages of method development 8.3.2 Selected detailed examples of environmental and other HILIC applications 8.3.2.1 Metals and their related organic compounds 8.3.2.2 Pharmaceutical compounds in aqueous environmental samples 8.3.2.3 Other applications 8.4 Conclusions Chapter 9. Theory and Practice of Two-Dimensional Liquid Chromatography Separations Involving the HILIC Mode of Separation 9.1 Fundamentals of multi-dimensional liquid chromatography 9.1.1 Scope 9.1.2 Potential advantages of two-dimensional separations over conventional separations 9.1.3 Modes of 2D separation 9.1.3.1 Offline fraction transfer 9.1.3.2 Online fraction transfer 9.1.3.3 Conceptual comparison of different 2D separation modes 9.1.4 Undersampling 9.1.5 Orthogonality 9.2 Complementarity of HILIC selectivity to other separation modes 9.3 Instrumentation and Experimental Considerations 9.3.1 Online versus offline 2DLC 9.3.1.1 Offline 2DLC 9.3.1.2 Online 2DLC 9.3.2 Dealing with solvent incompatibility 9.3.2.1 Partial mobile phase evaporation 9.3.2.2 Consideration of fraction transfer volume relative to the second dimension column volume 9.3.2.3 On-column focusing 9.3.3 Fast Separations 9.3.3.1 General considerations for fast LC separations 9.3.3.2 Fast HILIC separations 9.4 Applications 9.5 The future of HILIC separations in 2DLC.
Record Nr. UNINA-9910141530703321
Hoboken, N.J., : John Wiley & Sons, Inc., 2013
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Hydrophilic interaction chromatography : a guide for practitioners / / edited by Bernard A. Olsen, Brian W. Pack
Hydrophilic interaction chromatography : a guide for practitioners / / edited by Bernard A. Olsen, Brian W. Pack
Edizione [1st ed.]
Pubbl/distr/stampa Hoboken, N.J., : John Wiley & Sons, Inc., 2013
Descrizione fisica 1 online resource (337 p.)
Disciplina 543/.84
Altri autori (Persone) OlsenBernard A. <1953->
PackBrian W. <1970->
Collana Chemical analysis : a series of monographs on analytical chemistry and its applications
Soggetto topico Hydrophilic interaction liquid chromatography
ISBN 1-118-49524-1
1-283-97811-3
1-118-49521-7
1-118-49523-3
Classificazione SCI013010
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Machine generated contents note: Chapter 1. Separation Mechanisms in Hydrophilic Interaction Chromatography 1.1 Introduction 1.2 Historical Background. Recognition of the contribution of partition, ion exchange and reversed-phase interactions to the retention process 1.3 Recent studies on the contributory mechanisms to HILIC retention 1.3.1 Overview 1.3.2 Contribution of adsorption and partition to HILIC separations 1.3.3 Further studies on the contribution of ionic retention in HILIC 1.3.3.1 Introduction 1.3.3.2 Mobile phase considerations for the separation of ionogenic compounds 1.3.3.3. Ionisation state of the column as a function of pH 1.3.3.4 Quantitation of ionic retention effects on different columns 1.3.4 Reversed-phase retention on bare silica 1.3.5 Electrostatic Repulsion Hydrophilic Interaction Chromatography (ERLIC)- a new separation mode in HILIC. 1.4 Conclusions Chapter 2. Stationary Phases for HILIC 2.1 Introduction 2.2 HILIC stationary phases 2.2.1 Underivatized silica 2.2.1.1 Totally porous silica particles 2.2.1.2 Superficially porous (core shell) silica particles 2.2.1.3 Monolithic silica 2.2.1.4 Ethylene Bridged Hybrids (BEH) 2.2.2 Derivatized silica 2.2.2.1 Neutral derivatized silica 2.2.2.2 Zwitterionic derivatized silica 2.2.2.3 Positively charged derivatized silica 2.2.2.4 Negatively charged derivatized silica 2.2.3 Non-silica phases 2.2.3.1 Amino phases 2.2.3.2 Sulfonated S-DVB phases 2.3 Commercial HILIC phases 2.3.1 Efficiency comparison 2.3.2 Retention and selectivity comparisons 2.4 Conclusions Chapter 3. HILIC Method Development 3.1 Introduction 3.2 General method development considerations 3.2.1 Method objectives 3.2.2 Sample consideration 3.2.3 Systematic method development 3.3 Method development strategies 3.3.1 Systematic approach to column screening 3.3.2 Optimization of method parameters 3.3.2.1 Final column selection 3.3.2.2 Organic solvents 3.3.2.3 Mobile phase pH 3.3.2.4 Buffer types and concentration 3.3.2.5 Column temperature 3.3.2.6 Sample solvents 3.4 Detection for HILIC methods 3.4.1 Mass Spectrometry detector (MS) 3.4.2 Charged aerosol detector (CAD) 3.5 Conclusions Chapter 4. Pharmaceutical Applications of Hydrophilic Interaction Chromatography 4.1 Introduction 4.1.1 Definition of the problem 4.1.2 Selection of conditions 4.1.3 Validation of the method 4.1.4 General references 4.2 Determination of Counterions 4.2.1 Salt selection and options for counterion determination 4.2.2 Specific counterion analysis 4.2.3 Counterion screening with gradient elution 4.2.4 Suitable reference standards for counterion analysis 4.3 Main Component Methods 4.3.1 Potency/assay methods 4.3.2 Equipment cleaning verification assays 4.3.3 Dissolution methods 4.4 Determination of Impurities 4.4.1 Impurity screening and orthogonal separations 4.4.2 Impurity identification 4.4.3 Specific impurity determination 4.4.3.1 Pyrimidines, purines, nucleosides 4.4.3.2 Hydrazines with ethanol as weak solvent 4.4.3.3 Neutral and charged polar impurities in a drug substance 4.4.3.4 Polar basic compounds and impurities 4.4.4 Statistical design of experiments (DOE) for optimization 4.5 Excipients 4.5.1 Parenteral and solution formulations 4.5.2 Tablets, capsules and inhalation products 4.5.3 Sugars 4.5.4 Stabilizers and antioxidants 4.6 Chiral Applications 4.6.1 Chiral selectors and HILIC 4.6.1.1 Cyclodextrins 4.6.1.2 Macrocyclic antibiotics 4.6.1.3 Chiral crown ethers 4.6.1.4 Cyclofructans 4.6.2 Conclusions for chiral separations 4.7 Conclusions Chapter 5. Hydrophilic Interaction Chromatography (HILIC) for Drug Discovery 5.1 Drug Discovery Model 5.2 HILIC Applications for in vitro Biology 5.2.1 Biological screening and hit finding 5.2.1.1 Target selection and assay validation 5.2.1.2 High-throughput screening 5.2.2 New drug discovery strategies 5.3 HILIC Applications and Advances for Discovery Chemistry 5.3.1 Lead identification 5.3.2 Lead optimization 5.3.2.1 ADME profile 5.3.2.2 Biopharmaceutics 5.3.2.3 Chiral purity 5.3.3 Candidate selection 5.4 Practical Considerations 5.5 Conclusions Chapter 6. Advances in Hydrophilic Interaction Chromatography (HILIC) for Biochemical Applications 6.1 Introduction 6.2 Carbohydrates 6.2.1 Mono- and disaccharides 6.2.2 Oligosaccharides and polysaccharides 6.2.3 Glycans 6.2.3.1 Glycan and glycopeptide analysis 6.2.3.2 HILIC for sample enrichment 6.3 Nucleobases and Nucleosides 6.4 Oligonucleotides 6.5 Amino Acids and Peptides 6.6 Proteins 6.7 Phospholipids 6.8 Conclusions Chapter 7. HILIC-MS for Targeted Metabolomics and Small Molecule Bioanalysis 7.1 Introduction 7.2 The role of HILIC-MS in targeted metabolomics versus other LC modes 7.3 Strategies for method development based on retention behavior of targeted metabolites on HILIC stationary phases 7.3.1 Retention behavior of metabolites on HILIC stationary phases 7.3.2 Robustness, mobile phase compositions, and matrix effects 7.4 Summary Chapter 8. HILIC for Food, Environmental, and Other Applications 8.1 Introduction 8.2 Food applications for HILIC 8.2.1 Review of HILIC analytical methods for food analysis 8.2.1.1 Sample preparation in HILIC methods applied to food matrices 8.2.1.2 HILIC methods applied to food matrices: chromatographic parameters and detection 8.2.2 Selected detailed examples of HILIC applications in food analysis 8.2.2.1 Melamine (MEL) and cyanuric acid (CYA) 8.2.2.2 Water soluble vitamins 8.2.2.3 Seafood and other toxins 8.3 Environmental and other applications of HILIC 8.3.1 Review of environmental applications based on the stages of method development 8.3.2 Selected detailed examples of environmental and other HILIC applications 8.3.2.1 Metals and their related organic compounds 8.3.2.2 Pharmaceutical compounds in aqueous environmental samples 8.3.2.3 Other applications 8.4 Conclusions Chapter 9. Theory and Practice of Two-Dimensional Liquid Chromatography Separations Involving the HILIC Mode of Separation 9.1 Fundamentals of multi-dimensional liquid chromatography 9.1.1 Scope 9.1.2 Potential advantages of two-dimensional separations over conventional separations 9.1.3 Modes of 2D separation 9.1.3.1 Offline fraction transfer 9.1.3.2 Online fraction transfer 9.1.3.3 Conceptual comparison of different 2D separation modes 9.1.4 Undersampling 9.1.5 Orthogonality 9.2 Complementarity of HILIC selectivity to other separation modes 9.3 Instrumentation and Experimental Considerations 9.3.1 Online versus offline 2DLC 9.3.1.1 Offline 2DLC 9.3.1.2 Online 2DLC 9.3.2 Dealing with solvent incompatibility 9.3.2.1 Partial mobile phase evaporation 9.3.2.2 Consideration of fraction transfer volume relative to the second dimension column volume 9.3.2.3 On-column focusing 9.3.3 Fast Separations 9.3.3.1 General considerations for fast LC separations 9.3.3.2 Fast HILIC separations 9.4 Applications 9.5 The future of HILIC separations in 2DLC.
Record Nr. UNINA-9910810162203321
Hoboken, N.J., : John Wiley & Sons, Inc., 2013
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui