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Handbook of single molecule fluorescence spectroscopy [[electronic resource] /] / Chris Gell, David Brockwell, Alastair Smith
| Handbook of single molecule fluorescence spectroscopy [[electronic resource] /] / Chris Gell, David Brockwell, Alastair Smith |
| Autore | Gell Chris |
| Pubbl/distr/stampa | Oxford ; ; New York, : Oxford University Press, 2006 |
| Descrizione fisica | 1 online resource (279 p.) |
| Disciplina | 543/.56 |
| Altri autori (Persone) |
BrockwellDavid
SmithAlastair (D. Alastair) |
| Soggetto topico |
Fluorescence spectroscopy
Molecular spectroscopy |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-90302-3
0-19-152385-2 1-4294-9247-3 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Single molecule fluorescence techniques -- Single molecule fluorescence instrumentation -- Preparation of samples for single molecule fluorescence spectroscopy -- Fluorescence spectroscopy of freely diffusing single molecules : examples -- Fluorescence spectroscopy of immobilized single molecules : examples -- The outlook for single molecule fluorescence measurements. |
| Record Nr. | UNINA-9910450664303321 |
Gell Chris
|
||
| Oxford ; ; New York, : Oxford University Press, 2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Handbook of single molecule fluorescence spectroscopy [[electronic resource] /] / Chris Gell, David Brockwell, Alastair Smith
| Handbook of single molecule fluorescence spectroscopy [[electronic resource] /] / Chris Gell, David Brockwell, Alastair Smith |
| Autore | Gell Chris |
| Pubbl/distr/stampa | Oxford ; ; New York, : Oxford University Press, 2006 |
| Descrizione fisica | 1 online resource (279 p.) |
| Disciplina | 543/.56 |
| Altri autori (Persone) |
BrockwellDavid
SmithAlastair (D. Alastair) |
| Soggetto topico |
Fluorescence spectroscopy
Molecular spectroscopy |
| ISBN |
1-383-02487-1
1-280-90302-3 0-19-152385-2 1-4294-9247-3 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Single molecule fluorescence techniques -- Single molecule fluorescence instrumentation -- Preparation of samples for single molecule fluorescence spectroscopy -- Fluorescence spectroscopy of freely diffusing single molecules : examples -- Fluorescence spectroscopy of immobilized single molecules : examples -- The outlook for single molecule fluorescence measurements. |
| Record Nr. | UNINA-9910777055703321 |
|
Gell Chris
|
||
| Oxford ; ; New York, : Oxford University Press, 2006 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Metal-enhanced fluorescence [[electronic resource] /] / edited by Chris D. Geddes
| Metal-enhanced fluorescence [[electronic resource] /] / edited by Chris D. Geddes |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, c2010 |
| Descrizione fisica | 1 online resource (655 p.) |
| Disciplina | 543/.56 |
| Altri autori (Persone) | GeddesChris D |
| Soggetto topico |
Fluorescence spectroscopy
Nanoparticles Radioactive decay Plasmons (Physics) |
| ISBN |
1-282-65373-3
9786612653735 0-470-64279-3 0-470-64278-5 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
METAL-ENHANCED FLUORESCENCE; CONTENTS; Preface; Contributors; Metal-Enhanced Fluorescence: Progress Towards a Unified Plasmon-Fluorophore Description; Spectral Profile Modifications In Metal-Enhanced Fluorescence; The Role Of Plasmonic Engineering In Potential Surface-Enhanced Fluorescence; Importance of Spectral Overlap: Fluorescence Enhancement by Single Metal Nanoparticles; Near-IR Metal-Enhanced Fluorescence And Controlled Colloidal Aggregation; Optimisation Of Plasmonic Enhancement Of Fluorescence For Optical Biosensor Applications; Microwave-Accelerated Metal-Enhanced Fluorescence
Localized Surface Plasmon Coupled Fluorescence Fiber Optic Based BiosensingSurface Plasmon Enhanced Photochemistry; Metal-Enhanced Generation of Oxygen Rich Species; Synthesis Of Anisotropie Noble Metal Nanoparticles; Enhanced Fluorescence Detection Enabled By Zinc Oxide Nanomaterials; ZnO Platforms For Enhanced Directional Fluorescence Applications; E-Beam Lithography And Spontaneous Galvanic Displacement Reactions For Spatially Controlled MEF Applications; Metal-Enhanced Chemiluminescence; Enhanced Fluorescence From Gratings; Enhancing Fluorescence with Sub-Wavelength Metallic Apertures Enhanced Multi-Photon Excitation of Tryptophan-Silver ColloidPlasmon-enhanced radiative rates and applications to organic electronics; Fluorescent Quenching Gold Nanoparticles: Potential Biomedical Applications; Index |
| Record Nr. | UNINA-9910140557903321 |
| Hoboken, N.J., : Wiley, c2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Metal-enhanced fluorescence / / edited by Chris D. Geddes
| Metal-enhanced fluorescence / / edited by Chris D. Geddes |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : Wiley, c2010 |
| Descrizione fisica | 1 online resource (655 p.) |
| Disciplina | 543/.56 |
| Altri autori (Persone) | GeddesChris D |
| Soggetto topico |
Fluorescence spectroscopy
Nanoparticles Radioactive decay Plasmons (Physics) |
| ISBN |
9786612653735
9781282653733 1282653733 9780470642795 0470642793 9780470642788 0470642785 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
METAL-ENHANCED FLUORESCENCE; CONTENTS; Preface; Contributors; Metal-Enhanced Fluorescence: Progress Towards a Unified Plasmon-Fluorophore Description; Spectral Profile Modifications In Metal-Enhanced Fluorescence; The Role Of Plasmonic Engineering In Potential Surface-Enhanced Fluorescence; Importance of Spectral Overlap: Fluorescence Enhancement by Single Metal Nanoparticles; Near-IR Metal-Enhanced Fluorescence And Controlled Colloidal Aggregation; Optimisation Of Plasmonic Enhancement Of Fluorescence For Optical Biosensor Applications; Microwave-Accelerated Metal-Enhanced Fluorescence
Localized Surface Plasmon Coupled Fluorescence Fiber Optic Based BiosensingSurface Plasmon Enhanced Photochemistry; Metal-Enhanced Generation of Oxygen Rich Species; Synthesis Of Anisotropie Noble Metal Nanoparticles; Enhanced Fluorescence Detection Enabled By Zinc Oxide Nanomaterials; ZnO Platforms For Enhanced Directional Fluorescence Applications; E-Beam Lithography And Spontaneous Galvanic Displacement Reactions For Spatially Controlled MEF Applications; Metal-Enhanced Chemiluminescence; Enhanced Fluorescence From Gratings; Enhancing Fluorescence with Sub-Wavelength Metallic Apertures Enhanced Multi-Photon Excitation of Tryptophan-Silver ColloidPlasmon-enhanced radiative rates and applications to organic electronics; Fluorescent Quenching Gold Nanoparticles: Potential Biomedical Applications; Index |
| Record Nr. | UNINA-9910812202403321 |
| Hoboken, N.J., : Wiley, c2010 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani
| Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani |
| Autore | Albani Jihad René <1956-> |
| Pubbl/distr/stampa | Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 |
| Descrizione fisica | 1 online resource (270 p.) |
| Disciplina | 543/.56 |
| Soggetto topico | Fluorescence spectroscopy |
| ISBN |
1-281-31900-7
9786611319007 0-470-69205-7 0-470-69133-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 1.1 Introduction; 1.2 Characteristics of an Absorption Spectrum; 1.3 Beer-Lambert-Bouguer Law; 1.4 Effect of the Environment on Absorption Spectra; References; 2 Determination of the Calcofluor White Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 2.1 Introduction; 2.2 Biological Material Used; 2.2.1 Calcofluor White; 2.2.2 a1-Acid glycoprotein; 2.3 Experiments; 2.3.1 Absorption spectrum of Calcofluor free in PBS buffer
2.3.2 Determination of e. value of Calcofluor White free in PBS buffer 2.3.3 Determination of Calcofluor White e. value in the presence of a1-acid glycoprotein; 2.4 Solution; References; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 3.1 Objective of the Experiment; 3.2 Absorption Spectrum of NADH; 3.3 Absorption Spectrum of LDH; 3.4 Enzymatic Activity of LDH; 3.5 Kinetic Parameters; 3.6 Data and Results; 3.6.1 Determination of enzyme activity; 3.6.2 Determination of kinetic parameters; 3.7 Introduction to Kinetics and the Michaelis-Menten Equation; 3.7.1 Definitions 3.7.2 Reaction rates References; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 4.1 Introduction; 4.2 Solutions to be Prepared; 4.3 First-day Experiments; 4.3.1 Absorption spectrum of PNP; 4.3.2 Absorption of PNP as a function of pH; 4.3.3 Internal calibration of PNP; 4.3.4 Determination of B-galactosidase optimal pH; 4.3.5 Determination of B-galactosidase optimal temperature; 4.4 Second-day Experiments; 4.4.1 Kinetics of p-nitrophenyl-B-D-galactoside hydrolysis with B-galactosidase; 4.4.2 Determination of the B-galactosidase concentration in the test tube 4.5 Third-day Experiments 4.5.1 Determination of Km and Vmax of B-galactosidase; 4.5.2 Inhibiton of hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; 4.6 Fourth-day Experiments; 4.6.1 Effect of guanidine chloride concentration on B-galactosidase activity; 4.6.2 OD variation with guanidine chloride; 4.6.3 Mathematical derivation of Keq; 4.6.4 Definition of the standard Gibbs free energy, G.; 4.6.5 Relation between G. and G; 4.6.6 Relation between G. and Keq; 4.6.7 Effect of guanidine chloride on hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; References 5 Starch Hydrolysis by Amylase 5.1 Objectives; 5.2 Introduction; 5.3 Materials; 5.4 Procedures and Experiments; 5.4.1 Preparation of a 20 g lŠ1starch solution; 5.4.2 Calibration curve for starch concentration; 5.4.3 Calibration curve for sugar concentration; 5.4.4 Effect of pH; 5.4.5 Temperature effect; 5.4.6 Effect of heat treatment at 90.C; 5.4.7 Kinetics of starch hydrolysis; 5.4.8 Effect of inhibitor (CuCl2) on the amylase activity; 5.4.9 Effect of amylase concentration; 5.4.10 Complement experiments that can be performed; 5.4.11 Notes; References; 6 Determination of the pK of a Dye 6.1 Definition of pK |
| Record Nr. | UNINA-9910145261503321 |
|
Albani Jihad René <1956->
|
||
| Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani
| Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani |
| Autore | Albani Jihad René <1956-> |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 |
| Descrizione fisica | 1 online resource (270 p.) |
| Disciplina | 543/.56 |
| Soggetto topico | Fluorescence spectroscopy |
| ISBN |
1-281-31900-7
9786611319007 0-470-69205-7 0-470-69133-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 1.1 Introduction; 1.2 Characteristics of an Absorption Spectrum; 1.3 Beer-Lambert-Bouguer Law; 1.4 Effect of the Environment on Absorption Spectra; References; 2 Determination of the Calcofluor White Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 2.1 Introduction; 2.2 Biological Material Used; 2.2.1 Calcofluor White; 2.2.2 a1-Acid glycoprotein; 2.3 Experiments; 2.3.1 Absorption spectrum of Calcofluor free in PBS buffer
2.3.2 Determination of e. value of Calcofluor White free in PBS buffer 2.3.3 Determination of Calcofluor White e. value in the presence of a1-acid glycoprotein; 2.4 Solution; References; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 3.1 Objective of the Experiment; 3.2 Absorption Spectrum of NADH; 3.3 Absorption Spectrum of LDH; 3.4 Enzymatic Activity of LDH; 3.5 Kinetic Parameters; 3.6 Data and Results; 3.6.1 Determination of enzyme activity; 3.6.2 Determination of kinetic parameters; 3.7 Introduction to Kinetics and the Michaelis-Menten Equation; 3.7.1 Definitions 3.7.2 Reaction rates References; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 4.1 Introduction; 4.2 Solutions to be Prepared; 4.3 First-day Experiments; 4.3.1 Absorption spectrum of PNP; 4.3.2 Absorption of PNP as a function of pH; 4.3.3 Internal calibration of PNP; 4.3.4 Determination of B-galactosidase optimal pH; 4.3.5 Determination of B-galactosidase optimal temperature; 4.4 Second-day Experiments; 4.4.1 Kinetics of p-nitrophenyl-B-D-galactoside hydrolysis with B-galactosidase; 4.4.2 Determination of the B-galactosidase concentration in the test tube 4.5 Third-day Experiments 4.5.1 Determination of Km and Vmax of B-galactosidase; 4.5.2 Inhibiton of hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; 4.6 Fourth-day Experiments; 4.6.1 Effect of guanidine chloride concentration on B-galactosidase activity; 4.6.2 OD variation with guanidine chloride; 4.6.3 Mathematical derivation of Keq; 4.6.4 Definition of the standard Gibbs free energy, G.; 4.6.5 Relation between G. and G; 4.6.6 Relation between G. and Keq; 4.6.7 Effect of guanidine chloride on hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; References 5 Starch Hydrolysis by Amylase 5.1 Objectives; 5.2 Introduction; 5.3 Materials; 5.4 Procedures and Experiments; 5.4.1 Preparation of a 20 g lŠ1starch solution; 5.4.2 Calibration curve for starch concentration; 5.4.3 Calibration curve for sugar concentration; 5.4.4 Effect of pH; 5.4.5 Temperature effect; 5.4.6 Effect of heat treatment at 90.C; 5.4.7 Kinetics of starch hydrolysis; 5.4.8 Effect of inhibitor (CuCl2) on the amylase activity; 5.4.9 Effect of amylase concentration; 5.4.10 Complement experiments that can be performed; 5.4.11 Notes; References; 6 Determination of the pK of a Dye 6.1 Definition of pK |
| Record Nr. | UNISA-996218619003316 |
|
Albani Jihad René <1956->
|
||
| Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Principles and applications of fluorescence spectroscopy / / Jihad Rene Albani
| Principles and applications of fluorescence spectroscopy / / Jihad Rene Albani |
| Autore | Albani Jihad René <1956-> |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 |
| Descrizione fisica | 1 online resource (270 p.) |
| Disciplina | 543/.56 |
| Soggetto topico | Fluorescence spectroscopy |
| ISBN |
9786611319007
9781281319005 1281319007 9780470692059 0470692057 9780470691335 0470691336 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 1.1 Introduction; 1.2 Characteristics of an Absorption Spectrum; 1.3 Beer-Lambert-Bouguer Law; 1.4 Effect of the Environment on Absorption Spectra; References; 2 Determination of the Calcofluor White Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 2.1 Introduction; 2.2 Biological Material Used; 2.2.1 Calcofluor White; 2.2.2 a1-Acid glycoprotein; 2.3 Experiments; 2.3.1 Absorption spectrum of Calcofluor free in PBS buffer
2.3.2 Determination of e. value of Calcofluor White free in PBS buffer 2.3.3 Determination of Calcofluor White e. value in the presence of a1-acid glycoprotein; 2.4 Solution; References; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 3.1 Objective of the Experiment; 3.2 Absorption Spectrum of NADH; 3.3 Absorption Spectrum of LDH; 3.4 Enzymatic Activity of LDH; 3.5 Kinetic Parameters; 3.6 Data and Results; 3.6.1 Determination of enzyme activity; 3.6.2 Determination of kinetic parameters; 3.7 Introduction to Kinetics and the Michaelis-Menten Equation; 3.7.1 Definitions 3.7.2 Reaction rates References; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 4.1 Introduction; 4.2 Solutions to be Prepared; 4.3 First-day Experiments; 4.3.1 Absorption spectrum of PNP; 4.3.2 Absorption of PNP as a function of pH; 4.3.3 Internal calibration of PNP; 4.3.4 Determination of B-galactosidase optimal pH; 4.3.5 Determination of B-galactosidase optimal temperature; 4.4 Second-day Experiments; 4.4.1 Kinetics of p-nitrophenyl-B-D-galactoside hydrolysis with B-galactosidase; 4.4.2 Determination of the B-galactosidase concentration in the test tube 4.5 Third-day Experiments 4.5.1 Determination of Km and Vmax of B-galactosidase; 4.5.2 Inhibiton of hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; 4.6 Fourth-day Experiments; 4.6.1 Effect of guanidine chloride concentration on B-galactosidase activity; 4.6.2 OD variation with guanidine chloride; 4.6.3 Mathematical derivation of Keq; 4.6.4 Definition of the standard Gibbs free energy, G.; 4.6.5 Relation between G. and G; 4.6.6 Relation between G. and Keq; 4.6.7 Effect of guanidine chloride on hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; References 5 Starch Hydrolysis by Amylase 5.1 Objectives; 5.2 Introduction; 5.3 Materials; 5.4 Procedures and Experiments; 5.4.1 Preparation of a 20 g lŠ1starch solution; 5.4.2 Calibration curve for starch concentration; 5.4.3 Calibration curve for sugar concentration; 5.4.4 Effect of pH; 5.4.5 Temperature effect; 5.4.6 Effect of heat treatment at 90.C; 5.4.7 Kinetics of starch hydrolysis; 5.4.8 Effect of inhibitor (CuCl2) on the amylase activity; 5.4.9 Effect of amylase concentration; 5.4.10 Complement experiments that can be performed; 5.4.11 Notes; References; 6 Determination of the pK of a Dye 6.1 Definition of pK |
| Record Nr. | UNINA-9910824044703321 |
|
Albani Jihad René <1956->
|
||
| Oxford ; ; Ames, Iowa, : Blackwell Science, c2007 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Single molecule detection in solution : methods and applications
| Single molecule detection in solution : methods and applications |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Descrizione fisica | 1 online resource (377 pages) |
| Disciplina | 543/.56 |
| Soggetto topico |
Fluorescence spectroscopy
Raman spectroscopy Molecules Solution (Chemistry) Analytical Chemistry Chemistry Physical Sciences & Mathematics |
| ISBN |
1-280-55847-4
9786610558476 3-527-60080-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910146233103321 |
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Single molecule detection in solution : methods and applications
| Single molecule detection in solution : methods and applications |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Descrizione fisica | 1 online resource (377 pages) |
| Disciplina | 543/.56 |
| Soggetto topico |
Fluorescence spectroscopy
Raman spectroscopy Molecules Solution (Chemistry) Analytical Chemistry Chemistry Physical Sciences & Mathematics |
| ISBN |
1-280-55847-4
9786610558476 3-527-60080-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910830150003321 |
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Single molecule detection in solution : methods and applications
| Single molecule detection in solution : methods and applications |
| Pubbl/distr/stampa | [Place of publication not identified], : Wiley VCH, 2002 |
| Descrizione fisica | 1 online resource (377 pages) |
| Disciplina | 543/.56 |
| Soggetto topico |
Fluorescence spectroscopy
Raman spectroscopy Molecules Solution (Chemistry) Analytical Chemistry Chemistry Physical Sciences & Mathematics |
| ISBN |
1-280-55847-4
9786610558476 3-527-60080-9 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9911019401703321 |
| [Place of publication not identified], : Wiley VCH, 2002 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||