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Handbook of single molecule fluorescence spectroscopy [[electronic resource] /] / Chris Gell, David Brockwell, Alastair Smith
Handbook of single molecule fluorescence spectroscopy [[electronic resource] /] / Chris Gell, David Brockwell, Alastair Smith
Autore Gell Chris
Pubbl/distr/stampa Oxford ; ; New York, : Oxford University Press, 2006
Descrizione fisica 1 online resource (279 p.)
Disciplina 543/.56
Altri autori (Persone) BrockwellDavid
SmithAlastair (D. Alastair)
Soggetto topico Fluorescence spectroscopy
Molecular spectroscopy
Soggetto genere / forma Electronic books.
ISBN 1-280-90302-3
0-19-152385-2
1-4294-9247-3
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Single molecule fluorescence techniques -- Single molecule fluorescence instrumentation -- Preparation of samples for single molecule fluorescence spectroscopy -- Fluorescence spectroscopy of freely diffusing single molecules : examples -- Fluorescence spectroscopy of immobilized single molecules : examples -- The outlook for single molecule fluorescence measurements.
Record Nr. UNINA-9910450664303321
Gell Chris  
Oxford ; ; New York, : Oxford University Press, 2006
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Handbook of single molecule fluorescence spectroscopy [[electronic resource] /] / Chris Gell, David Brockwell, Alastair Smith
Handbook of single molecule fluorescence spectroscopy [[electronic resource] /] / Chris Gell, David Brockwell, Alastair Smith
Autore Gell Chris
Pubbl/distr/stampa Oxford ; ; New York, : Oxford University Press, 2006
Descrizione fisica 1 online resource (279 p.)
Disciplina 543/.56
Altri autori (Persone) BrockwellDavid
SmithAlastair (D. Alastair)
Soggetto topico Fluorescence spectroscopy
Molecular spectroscopy
ISBN 1-383-02487-1
1-280-90302-3
0-19-152385-2
1-4294-9247-3
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Single molecule fluorescence techniques -- Single molecule fluorescence instrumentation -- Preparation of samples for single molecule fluorescence spectroscopy -- Fluorescence spectroscopy of freely diffusing single molecules : examples -- Fluorescence spectroscopy of immobilized single molecules : examples -- The outlook for single molecule fluorescence measurements.
Record Nr. UNINA-9910777055703321
Gell Chris  
Oxford ; ; New York, : Oxford University Press, 2006
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Handbook of single molecule fluorescence spectroscopy / / Chris Gell, David Brockwell, Alastair Smith
Handbook of single molecule fluorescence spectroscopy / / Chris Gell, David Brockwell, Alastair Smith
Autore Gell Chris
Edizione [1st ed.]
Pubbl/distr/stampa Oxford ; ; New York, : Oxford University Press, 2006
Descrizione fisica 1 online resource (279 p.)
Disciplina 543/.56
Altri autori (Persone) BrockwellDavid
SmithAlastair (D. Alastair)
Collana Oxford scholarship online
Soggetto topico Fluorescence spectroscopy
Molecular spectroscopy
ISBN 1-383-02487-1
1-280-90302-3
0-19-152385-2
1-4294-9247-3
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Single molecule fluorescence techniques -- Single molecule fluorescence instrumentation -- Preparation of samples for single molecule fluorescence spectroscopy -- Fluorescence spectroscopy of freely diffusing single molecules : examples -- Fluorescence spectroscopy of immobilized single molecules : examples -- The outlook for single molecule fluorescence measurements.
Record Nr. UNINA-9910818995103321
Gell Chris  
Oxford ; ; New York, : Oxford University Press, 2006
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Metal-enhanced fluorescence [[electronic resource] /] / edited by Chris D. Geddes
Metal-enhanced fluorescence [[electronic resource] /] / edited by Chris D. Geddes
Pubbl/distr/stampa Hoboken, N.J., : Wiley, c2010
Descrizione fisica 1 online resource (655 p.)
Disciplina 543/.56
Altri autori (Persone) GeddesChris D
Soggetto topico Fluorescence spectroscopy
Nanoparticles
Radioactive decay
Plasmons (Physics)
ISBN 1-282-65373-3
9786612653735
0-470-64279-3
0-470-64278-5
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto METAL-ENHANCED FLUORESCENCE; CONTENTS; Preface; Contributors; Metal-Enhanced Fluorescence: Progress Towards a Unified Plasmon-Fluorophore Description; Spectral Profile Modifications In Metal-Enhanced Fluorescence; The Role Of Plasmonic Engineering In Potential Surface-Enhanced Fluorescence; Importance of Spectral Overlap: Fluorescence Enhancement by Single Metal Nanoparticles; Near-IR Metal-Enhanced Fluorescence And Controlled Colloidal Aggregation; Optimisation Of Plasmonic Enhancement Of Fluorescence For Optical Biosensor Applications; Microwave-Accelerated Metal-Enhanced Fluorescence
Localized Surface Plasmon Coupled Fluorescence Fiber Optic Based BiosensingSurface Plasmon Enhanced Photochemistry; Metal-Enhanced Generation of Oxygen Rich Species; Synthesis Of Anisotropie Noble Metal Nanoparticles; Enhanced Fluorescence Detection Enabled By Zinc Oxide Nanomaterials; ZnO Platforms For Enhanced Directional Fluorescence Applications; E-Beam Lithography And Spontaneous Galvanic Displacement Reactions For Spatially Controlled MEF Applications; Metal-Enhanced Chemiluminescence; Enhanced Fluorescence From Gratings; Enhancing Fluorescence with Sub-Wavelength Metallic Apertures
Enhanced Multi-Photon Excitation of Tryptophan-Silver ColloidPlasmon-enhanced radiative rates and applications to organic electronics; Fluorescent Quenching Gold Nanoparticles: Potential Biomedical Applications; Index
Record Nr. UNINA-9910140557903321
Hoboken, N.J., : Wiley, c2010
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Metal-enhanced fluorescence / / edited by Chris D. Geddes
Metal-enhanced fluorescence / / edited by Chris D. Geddes
Edizione [1st ed.]
Pubbl/distr/stampa Hoboken, N.J., : Wiley, c2010
Descrizione fisica 1 online resource (655 p.)
Disciplina 543/.56
Altri autori (Persone) GeddesChris D
Soggetto topico Fluorescence spectroscopy
Nanoparticles
Radioactive decay
Plasmons (Physics)
ISBN 1-282-65373-3
9786612653735
0-470-64279-3
0-470-64278-5
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto METAL-ENHANCED FLUORESCENCE; CONTENTS; Preface; Contributors; Metal-Enhanced Fluorescence: Progress Towards a Unified Plasmon-Fluorophore Description; Spectral Profile Modifications In Metal-Enhanced Fluorescence; The Role Of Plasmonic Engineering In Potential Surface-Enhanced Fluorescence; Importance of Spectral Overlap: Fluorescence Enhancement by Single Metal Nanoparticles; Near-IR Metal-Enhanced Fluorescence And Controlled Colloidal Aggregation; Optimisation Of Plasmonic Enhancement Of Fluorescence For Optical Biosensor Applications; Microwave-Accelerated Metal-Enhanced Fluorescence
Localized Surface Plasmon Coupled Fluorescence Fiber Optic Based BiosensingSurface Plasmon Enhanced Photochemistry; Metal-Enhanced Generation of Oxygen Rich Species; Synthesis Of Anisotropie Noble Metal Nanoparticles; Enhanced Fluorescence Detection Enabled By Zinc Oxide Nanomaterials; ZnO Platforms For Enhanced Directional Fluorescence Applications; E-Beam Lithography And Spontaneous Galvanic Displacement Reactions For Spatially Controlled MEF Applications; Metal-Enhanced Chemiluminescence; Enhanced Fluorescence From Gratings; Enhancing Fluorescence with Sub-Wavelength Metallic Apertures
Enhanced Multi-Photon Excitation of Tryptophan-Silver ColloidPlasmon-enhanced radiative rates and applications to organic electronics; Fluorescent Quenching Gold Nanoparticles: Potential Biomedical Applications; Index
Record Nr. UNINA-9910812202403321
Hoboken, N.J., : Wiley, c2010
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani
Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani
Autore Albani Jihad René <1956->
Pubbl/distr/stampa Oxford ; ; Ames, Iowa, : Blackwell Science, c2007
Descrizione fisica 1 online resource (270 p.)
Disciplina 543/.56
Soggetto topico Fluorescence spectroscopy
ISBN 1-281-31900-7
9786611319007
0-470-69205-7
0-470-69133-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 1.1 Introduction; 1.2 Characteristics of an Absorption Spectrum; 1.3 Beer-Lambert-Bouguer Law; 1.4 Effect of the Environment on Absorption Spectra; References; 2 Determination of the Calcofluor White Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 2.1 Introduction; 2.2 Biological Material Used; 2.2.1 Calcofluor White; 2.2.2 a1-Acid glycoprotein; 2.3 Experiments; 2.3.1 Absorption spectrum of Calcofluor free in PBS buffer
2.3.2 Determination of e. value of Calcofluor White free in PBS buffer 2.3.3 Determination of Calcofluor White e. value in the presence of a1-acid glycoprotein; 2.4 Solution; References; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 3.1 Objective of the Experiment; 3.2 Absorption Spectrum of NADH; 3.3 Absorption Spectrum of LDH; 3.4 Enzymatic Activity of LDH; 3.5 Kinetic Parameters; 3.6 Data and Results; 3.6.1 Determination of enzyme activity; 3.6.2 Determination of kinetic parameters; 3.7 Introduction to Kinetics and the Michaelis-Menten Equation; 3.7.1 Definitions
3.7.2 Reaction rates References; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 4.1 Introduction; 4.2 Solutions to be Prepared; 4.3 First-day Experiments; 4.3.1 Absorption spectrum of PNP; 4.3.2 Absorption of PNP as a function of pH; 4.3.3 Internal calibration of PNP; 4.3.4 Determination of B-galactosidase optimal pH; 4.3.5 Determination of B-galactosidase optimal temperature; 4.4 Second-day Experiments; 4.4.1 Kinetics of p-nitrophenyl-B-D-galactoside hydrolysis with B-galactosidase; 4.4.2 Determination of the B-galactosidase concentration in the test tube
4.5 Third-day Experiments 4.5.1 Determination of Km and Vmax of B-galactosidase; 4.5.2 Inhibiton of hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; 4.6 Fourth-day Experiments; 4.6.1 Effect of guanidine chloride concentration on B-galactosidase activity; 4.6.2 OD variation with guanidine chloride; 4.6.3 Mathematical derivation of Keq; 4.6.4 Definition of the standard Gibbs free energy, G.; 4.6.5 Relation between G. and G; 4.6.6 Relation between G. and Keq; 4.6.7 Effect of guanidine chloride on hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; References
5 Starch Hydrolysis by Amylase 5.1 Objectives; 5.2 Introduction; 5.3 Materials; 5.4 Procedures and Experiments; 5.4.1 Preparation of a 20 g lŠ1starch solution; 5.4.2 Calibration curve for starch concentration; 5.4.3 Calibration curve for sugar concentration; 5.4.4 Effect of pH; 5.4.5 Temperature effect; 5.4.6 Effect of heat treatment at 90.C; 5.4.7 Kinetics of starch hydrolysis; 5.4.8 Effect of inhibitor (CuCl2) on the amylase activity; 5.4.9 Effect of amylase concentration; 5.4.10 Complement experiments that can be performed; 5.4.11 Notes; References; 6 Determination of the pK of a Dye
6.1 Definition of pK
Record Nr. UNINA-9910145261503321
Albani Jihad René <1956->  
Oxford ; ; Ames, Iowa, : Blackwell Science, c2007
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani
Principles and applications of fluorescence spectroscopy [[electronic resource] /] / Jihad René Albani
Autore Albani Jihad René <1956->
Edizione [1st ed.]
Pubbl/distr/stampa Oxford ; ; Ames, Iowa, : Blackwell Science, c2007
Descrizione fisica 1 online resource (270 p.)
Disciplina 543/.56
Soggetto topico Fluorescence spectroscopy
ISBN 1-281-31900-7
9786611319007
0-470-69205-7
0-470-69133-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 1.1 Introduction; 1.2 Characteristics of an Absorption Spectrum; 1.3 Beer-Lambert-Bouguer Law; 1.4 Effect of the Environment on Absorption Spectra; References; 2 Determination of the Calcofluor White Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 2.1 Introduction; 2.2 Biological Material Used; 2.2.1 Calcofluor White; 2.2.2 a1-Acid glycoprotein; 2.3 Experiments; 2.3.1 Absorption spectrum of Calcofluor free in PBS buffer
2.3.2 Determination of e. value of Calcofluor White free in PBS buffer 2.3.3 Determination of Calcofluor White e. value in the presence of a1-acid glycoprotein; 2.4 Solution; References; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 3.1 Objective of the Experiment; 3.2 Absorption Spectrum of NADH; 3.3 Absorption Spectrum of LDH; 3.4 Enzymatic Activity of LDH; 3.5 Kinetic Parameters; 3.6 Data and Results; 3.6.1 Determination of enzyme activity; 3.6.2 Determination of kinetic parameters; 3.7 Introduction to Kinetics and the Michaelis-Menten Equation; 3.7.1 Definitions
3.7.2 Reaction rates References; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 4.1 Introduction; 4.2 Solutions to be Prepared; 4.3 First-day Experiments; 4.3.1 Absorption spectrum of PNP; 4.3.2 Absorption of PNP as a function of pH; 4.3.3 Internal calibration of PNP; 4.3.4 Determination of B-galactosidase optimal pH; 4.3.5 Determination of B-galactosidase optimal temperature; 4.4 Second-day Experiments; 4.4.1 Kinetics of p-nitrophenyl-B-D-galactoside hydrolysis with B-galactosidase; 4.4.2 Determination of the B-galactosidase concentration in the test tube
4.5 Third-day Experiments 4.5.1 Determination of Km and Vmax of B-galactosidase; 4.5.2 Inhibiton of hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; 4.6 Fourth-day Experiments; 4.6.1 Effect of guanidine chloride concentration on B-galactosidase activity; 4.6.2 OD variation with guanidine chloride; 4.6.3 Mathematical derivation of Keq; 4.6.4 Definition of the standard Gibbs free energy, G.; 4.6.5 Relation between G. and G; 4.6.6 Relation between G. and Keq; 4.6.7 Effect of guanidine chloride on hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; References
5 Starch Hydrolysis by Amylase 5.1 Objectives; 5.2 Introduction; 5.3 Materials; 5.4 Procedures and Experiments; 5.4.1 Preparation of a 20 g lŠ1starch solution; 5.4.2 Calibration curve for starch concentration; 5.4.3 Calibration curve for sugar concentration; 5.4.4 Effect of pH; 5.4.5 Temperature effect; 5.4.6 Effect of heat treatment at 90.C; 5.4.7 Kinetics of starch hydrolysis; 5.4.8 Effect of inhibitor (CuCl2) on the amylase activity; 5.4.9 Effect of amylase concentration; 5.4.10 Complement experiments that can be performed; 5.4.11 Notes; References; 6 Determination of the pK of a Dye
6.1 Definition of pK
Record Nr. UNISA-996218619003316
Albani Jihad René <1956->  
Oxford ; ; Ames, Iowa, : Blackwell Science, c2007
Materiale a stampa
Lo trovi qui: Univ. di Salerno
Opac: Controlla la disponibilità qui
Principles and applications of fluorescence spectroscopy / / Jihad Rene Albani
Principles and applications of fluorescence spectroscopy / / Jihad Rene Albani
Autore Albani Jihad Rene <1956->
Edizione [1st ed.]
Pubbl/distr/stampa Oxford ; ; Ames, Iowa, : Blackwell Science, c2007
Descrizione fisica 1 online resource (270 p.)
Disciplina 543/.56
Soggetto topico Fluorescence spectroscopy
ISBN 1-281-31900-7
9786611319007
0-470-69205-7
0-470-69133-6
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 1.1 Introduction; 1.2 Characteristics of an Absorption Spectrum; 1.3 Beer-Lambert-Bouguer Law; 1.4 Effect of the Environment on Absorption Spectra; References; 2 Determination of the Calcofluor White Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 2.1 Introduction; 2.2 Biological Material Used; 2.2.1 Calcofluor White; 2.2.2 a1-Acid glycoprotein; 2.3 Experiments; 2.3.1 Absorption spectrum of Calcofluor free in PBS buffer
2.3.2 Determination of e. value of Calcofluor White free in PBS buffer 2.3.3 Determination of Calcofluor White e. value in the presence of a1-acid glycoprotein; 2.4 Solution; References; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 3.1 Objective of the Experiment; 3.2 Absorption Spectrum of NADH; 3.3 Absorption Spectrum of LDH; 3.4 Enzymatic Activity of LDH; 3.5 Kinetic Parameters; 3.6 Data and Results; 3.6.1 Determination of enzyme activity; 3.6.2 Determination of kinetic parameters; 3.7 Introduction to Kinetics and the Michaelis-Menten Equation; 3.7.1 Definitions
3.7.2 Reaction rates References; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 4.1 Introduction; 4.2 Solutions to be Prepared; 4.3 First-day Experiments; 4.3.1 Absorption spectrum of PNP; 4.3.2 Absorption of PNP as a function of pH; 4.3.3 Internal calibration of PNP; 4.3.4 Determination of B-galactosidase optimal pH; 4.3.5 Determination of B-galactosidase optimal temperature; 4.4 Second-day Experiments; 4.4.1 Kinetics of p-nitrophenyl-B-D-galactoside hydrolysis with B-galactosidase; 4.4.2 Determination of the B-galactosidase concentration in the test tube
4.5 Third-day Experiments 4.5.1 Determination of Km and Vmax of B-galactosidase; 4.5.2 Inhibiton of hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; 4.6 Fourth-day Experiments; 4.6.1 Effect of guanidine chloride concentration on B-galactosidase activity; 4.6.2 OD variation with guanidine chloride; 4.6.3 Mathematical derivation of Keq; 4.6.4 Definition of the standard Gibbs free energy, G.; 4.6.5 Relation between G. and G; 4.6.6 Relation between G. and Keq; 4.6.7 Effect of guanidine chloride on hydrolysis kinetics of p-nitrophenyl-B-D-galactoside; References
5 Starch Hydrolysis by Amylase 5.1 Objectives; 5.2 Introduction; 5.3 Materials; 5.4 Procedures and Experiments; 5.4.1 Preparation of a 20 g lŠ1starch solution; 5.4.2 Calibration curve for starch concentration; 5.4.3 Calibration curve for sugar concentration; 5.4.4 Effect of pH; 5.4.5 Temperature effect; 5.4.6 Effect of heat treatment at 90.C; 5.4.7 Kinetics of starch hydrolysis; 5.4.8 Effect of inhibitor (CuCl2) on the amylase activity; 5.4.9 Effect of amylase concentration; 5.4.10 Complement experiments that can be performed; 5.4.11 Notes; References; 6 Determination of the pK of a Dye
6.1 Definition of pK
Record Nr. UNINA-9910824044703321
Albani Jihad Rene <1956->  
Oxford ; ; Ames, Iowa, : Blackwell Science, c2007
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Single molecule detection in solution : methods and applications
Single molecule detection in solution : methods and applications
Pubbl/distr/stampa [Place of publication not identified], : Wiley VCH, 2002
Descrizione fisica 1 online resource (377 pages)
Disciplina 543/.56
Soggetto topico Fluorescence spectroscopy
Raman spectroscopy
Molecules
Solution (Chemistry)
Analytical Chemistry
Chemistry
Physical Sciences & Mathematics
ISBN 1-280-55847-4
9786610558476
3-527-60080-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910146233103321
[Place of publication not identified], : Wiley VCH, 2002
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Single molecule detection in solution : methods and applications
Single molecule detection in solution : methods and applications
Pubbl/distr/stampa [Place of publication not identified], : Wiley VCH, 2002
Descrizione fisica 1 online resource (377 pages)
Disciplina 543/.56
Soggetto topico Fluorescence spectroscopy
Raman spectroscopy
Molecules
Solution (Chemistry)
Analytical Chemistry
Chemistry
Physical Sciences & Mathematics
ISBN 1-280-55847-4
9786610558476
3-527-60080-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Record Nr. UNINA-9910830150003321
[Place of publication not identified], : Wiley VCH, 2002
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui