Info
- Utilizzare la checkbox di selezione a fianco di ciascun documento per attivare le funzionalità di stampa, invio email, download nei formati disponibili del (i) record.
Ultra-high performance liquid chromatography and its applications [[electronic resource] /] / edited by Quanyun Alan Xu
| Ultra-high performance liquid chromatography and its applications [[electronic resource] /] / edited by Quanyun Alan Xu |
| Pubbl/distr/stampa | Hoboken, N.J., : John Wiley & Sons Inc., 2013 |
| Descrizione fisica | 1 online resource (306 p.) |
| Disciplina | 543/.84 |
| Altri autori (Persone) | XuQuanyun A. <1961-> |
| Soggetto topico | Liquid chromatography |
| ISBN |
1-118-53398-4
1-118-53395-X 1-299-46494-7 1-118-53399-2 |
| Classificazione | TEC009010 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Ultra-High Performance Liquid Chromatography and Its Applications; Contents; Preface; Contributors; Chapter 1 UHPLC Method Development; 1.1 INTRODUCTION; 1.2 METHOD DEVELOPMENT; 1.2.1 Gather Sample Information and Define Method Goals; 1.2.2 Scout Columns and Mobile Phases; 1.2.3 Analyze Scouting Results and Select Separation Conditions; 1.2.4 Optimize the Method; 1.2.5 Validate the Method; 1.2.6 Phase-Appropriate Method Development; 1.2.7 Very High Pressure and Frictional Heating in UHPLC; 1.2.8 Relevance of Various Instrumentation to Method Development
1.2.9 Method Resolution and Speed Requirements1.2.10 Example 1.1; 1.2.11 Example 1.2; 1.3 CONVERSION OF AN HPLC METHOD TO UHPLC; 1.3.1 Select a UHPLC Column with the Same Chemistry; 1.3.2 Measure Dwell Volumes of HPLC and UHPLC Systems; 1.3.3 Calculate Injection Volume, Flow Rate, Holding Time, and Gradient Segment Time; 1.3.4 Perform Verification Experiment; 1.3.5 Validate the Method; 1.3.6 Example 1.3; 1.3.7 Example 1.4; 1.4 CONVERSION OF A UHPLC METHOD TO HPLC; 1.5 SUMMARY; REFERENCES; Chapter 2 Method Transfer Between HPLC and UHPLC Platforms; 2.1 INTRODUCTION 2.2 TRANSFERRING HPLC METHODS TO UHPLC2.2.1 Fundamental Physical Relationships; 2.2.2 Types of UHPLC Applications; 2.2.3 Additional Fundamental Considerations; 2.2.4 Transfer of Isocratic Methods from HPLC to UHPLC; 2.2.5 Cautions on Transferring Isocratic Methods from HPLC to UHPLC; 2.2.6 Transfer of Gradient Methods from HPLC to UHPLC; 2.2.7 Cautions on the Scaling of Gradient Methods from HPLC to UHPLC; 2.2.8 Case Studies and Recommendations; 2.2.9 Final Discussion of Practical Challenges; 2.2.10 Conclusions on the Transfer of Methods from HPLC to UHPLC 2.3 TRANSFERRING UHPLC METHODS TO HPLC PLATFORMS2.3.1 UHPLC to Traditional HPLC Columns; 2.3.2 UHPLC to Superficially Porous Particle HPLC Columns; 2.3.3 UHPLC to HPLC Method Transfer; 2.4 TRANSFERRING LC METHODS TO OTHER LABS; 2.4.1 UHPLC Instrument Issues Encountered During Method Transfers; 2.4.2 Back to LC Basics; REFERENCES; Chapter 3 Practical Aspects of Ultrahigh Performance Liquid Chromatography; 3.1 INTRODUCTION; 3.2 EFFECT OF EXTRA-COLUMN VOLUME ON PERFORMANCE OF SUB-2 lM PARTICLE-PACKED COLUMNS IN UHPLC; 3.2.1 Theoretical Considerations 3.2.2 Extra-Column Volumes and Column Volumes3.2.3 Effect of Extra-Column Volume on Performance; 3.3 COLUMN PRESSURE AND FRICTIONAL HEATING; 3.3.1 Column Pressure vs. Particle Size; 3.3.2 Phenomenon of Frictional Heating; 3.3.3 Effect of Frictional Heating on Retention Factor; 3.3.4 Factors Leading to Frictional Heating; 3.3.5 How to Resolve Thermal Gradient Issues; 3.3.6 Effect of Pressure on Mobile Phase Characteristics; 3.3.7 Effect of Pressure on Retention Factor with Minimal Frictional Heating; 3.4 METHOD TRANSFER BETWEEN UHPLC AND HPLC AND OTHER TIPS 3.4.1 Column Dimension and Particle Size |
| Record Nr. | UNINA-9910139018103321 |
| Hoboken, N.J., : John Wiley & Sons Inc., 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Ultra-high performance liquid chromatography and its applications / / edited by Quanyun Alan Xu
| Ultra-high performance liquid chromatography and its applications / / edited by Quanyun Alan Xu |
| Edizione | [1st ed.] |
| Pubbl/distr/stampa | Hoboken, N.J., : John Wiley & Sons Inc., 2013 |
| Descrizione fisica | 1 online resource (306 p.) |
| Disciplina | 543/.84 |
| Altri autori (Persone) | XuQuanyun A. <1961-> |
| Soggetto topico | Liquid chromatography |
| ISBN |
9781118533987
1118533984 9781118533956 111853395X 9781299464940 1299464947 9781118533994 1118533992 |
| Classificazione | TEC009010 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Ultra-High Performance Liquid Chromatography and Its Applications; Contents; Preface; Contributors; Chapter 1 UHPLC Method Development; 1.1 INTRODUCTION; 1.2 METHOD DEVELOPMENT; 1.2.1 Gather Sample Information and Define Method Goals; 1.2.2 Scout Columns and Mobile Phases; 1.2.3 Analyze Scouting Results and Select Separation Conditions; 1.2.4 Optimize the Method; 1.2.5 Validate the Method; 1.2.6 Phase-Appropriate Method Development; 1.2.7 Very High Pressure and Frictional Heating in UHPLC; 1.2.8 Relevance of Various Instrumentation to Method Development
1.2.9 Method Resolution and Speed Requirements1.2.10 Example 1.1; 1.2.11 Example 1.2; 1.3 CONVERSION OF AN HPLC METHOD TO UHPLC; 1.3.1 Select a UHPLC Column with the Same Chemistry; 1.3.2 Measure Dwell Volumes of HPLC and UHPLC Systems; 1.3.3 Calculate Injection Volume, Flow Rate, Holding Time, and Gradient Segment Time; 1.3.4 Perform Verification Experiment; 1.3.5 Validate the Method; 1.3.6 Example 1.3; 1.3.7 Example 1.4; 1.4 CONVERSION OF A UHPLC METHOD TO HPLC; 1.5 SUMMARY; REFERENCES; Chapter 2 Method Transfer Between HPLC and UHPLC Platforms; 2.1 INTRODUCTION 2.2 TRANSFERRING HPLC METHODS TO UHPLC2.2.1 Fundamental Physical Relationships; 2.2.2 Types of UHPLC Applications; 2.2.3 Additional Fundamental Considerations; 2.2.4 Transfer of Isocratic Methods from HPLC to UHPLC; 2.2.5 Cautions on Transferring Isocratic Methods from HPLC to UHPLC; 2.2.6 Transfer of Gradient Methods from HPLC to UHPLC; 2.2.7 Cautions on the Scaling of Gradient Methods from HPLC to UHPLC; 2.2.8 Case Studies and Recommendations; 2.2.9 Final Discussion of Practical Challenges; 2.2.10 Conclusions on the Transfer of Methods from HPLC to UHPLC 2.3 TRANSFERRING UHPLC METHODS TO HPLC PLATFORMS2.3.1 UHPLC to Traditional HPLC Columns; 2.3.2 UHPLC to Superficially Porous Particle HPLC Columns; 2.3.3 UHPLC to HPLC Method Transfer; 2.4 TRANSFERRING LC METHODS TO OTHER LABS; 2.4.1 UHPLC Instrument Issues Encountered During Method Transfers; 2.4.2 Back to LC Basics; REFERENCES; Chapter 3 Practical Aspects of Ultrahigh Performance Liquid Chromatography; 3.1 INTRODUCTION; 3.2 EFFECT OF EXTRA-COLUMN VOLUME ON PERFORMANCE OF SUB-2 lM PARTICLE-PACKED COLUMNS IN UHPLC; 3.2.1 Theoretical Considerations 3.2.2 Extra-Column Volumes and Column Volumes3.2.3 Effect of Extra-Column Volume on Performance; 3.3 COLUMN PRESSURE AND FRICTIONAL HEATING; 3.3.1 Column Pressure vs. Particle Size; 3.3.2 Phenomenon of Frictional Heating; 3.3.3 Effect of Frictional Heating on Retention Factor; 3.3.4 Factors Leading to Frictional Heating; 3.3.5 How to Resolve Thermal Gradient Issues; 3.3.6 Effect of Pressure on Mobile Phase Characteristics; 3.3.7 Effect of Pressure on Retention Factor with Minimal Frictional Heating; 3.4 METHOD TRANSFER BETWEEN UHPLC AND HPLC AND OTHER TIPS 3.4.1 Column Dimension and Particle Size |
| Record Nr. | UNINA-9910822370003321 |
| Hoboken, N.J., : John Wiley & Sons Inc., 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||