| Autore |
Xia Wenshui
|
| Edizione | [1st ed.] |
| Pubbl/distr/stampa |
New York, : Nova Science Publishers, c2010
|
| Descrizione fisica |
1 online resource (78 p.)
|
| Disciplina |
612.3/9
|
| Altri autori (Persone) |
LiuPing
|
| Collana |
Environmental Science, Engineering and Technology
|
| Soggetto topico |
Chitosan - Metabolism
|
| ISBN |
1-61324-220-4
|
| Formato |
Materiale a stampa  |
| Livello bibliografico |
Monografia |
| Lingua di pubblicazione |
eng
|
| Nota di contenuto |
Intro -- CHITOSAN HYDROLYSIS BY NON-SPECIFIC ENZYMES -- CHITOSAN HYDROLYSIS BY NON-SPECIFIC ENZYMES -- CONTENTS -- ABSTRACT -- Chapter 1 INTRODUCTION -- Chapter 2 CHARACTERIZATION OF CHITOSAN HYDROLYSIS BY NONSPECIFIC ENZYMES -- 2.1. CHARACTERIZATION OFCHITOSAN HYDROLYSIS BY NON-SPECIFIC CELLULASES -- (1) Temperature and pH -- (2) Molecular Weight -- (3) Reduction of the Viscosity -- (4) Kinetic Parameters -- (5) Metal Ions -- 2.2. CHARACTERIZATION OF CHITOSAN HYDROLYSIS BY NON-SPECIFIC LIPASE -- 2.3. Characterization of Chitosan Hydrolysis by Non-Specific Papain -- Chapter 3 MECHANISM OF NON-SPECIFIC ENZYMES TOWARD CHITOSAN -- 3.1. PURIFICATION AND CHARACTERIZATION OF CHITOSANOLYTIC COMPONENTS FROM NON -SPECIFIC ENZYMES -- 3.1.1. Purification and Characterization of a Bifunctional Enzyme with Chitosanolytic and Cellulolytic Activity from Commercial Cellulase -- 3.1.2. Purification and Characterization of a Bifunctional Chitosanase with Chitinase Activity from Commercial Lipase -- 3.2. ACTION MODE ANALYSIS OF BIFUNCTIONAL ENZYMES ON CHITOSAN -- 3.2.1. Substrate Specificity -- 3.2.2. Chitosan and Chitooligosaccharide Hydrolysis Analysis by CCBE -- 3.2.3. TLC Analysis of COS Hydrolysis Products by the Purified CNBE -- Chapter 4 STRUCTURE AND FUNCTION ANALYSIS OF BIFUNCTIONAL ENZYMES WITH CHITOSANOLYTIC ACTIVITY -- 4.1. AMINO ACID COMPOSITION OF PURIFIED CCBE AND CNBE -- 4.2. STRUCTURE ANALYSIS OF PURIFIED CCBE AND CNBE -- 4.2.1. N-Terminal Sequencing Analysis of CNBE -- 4.2.2. Sequencing Analysis of the Purified CCBE by MALDI-TOF Mass -- 4.3. DETERMINATION OF THE ACTIVE SITES OF CCBE AND CNBE BY CHEMICAL MODIFICATION -- 4.3.1. The Essential Carboxyl Group Modification using EDC -- 4.3.2. Modification of Tryptophan Residues using NBS -- 4.3.3. Modification of Histidine Residue using DEPC.
4.3.4. Modification of Tyrosine, Threonine/Serine, Arginine, Methionine Residues Etc -- Chapter 5 IDENTIFICATION OF CCBE FROM T.VIRIDE BY MOLECULAR CLONING -- 5.1. DETERMINATION OF FERMENTATION CONDITION OF T.VIRIDE -- 5.2. CLONING AND SEQUENCE ANALYSIS OF CCBE GENE -- 5.3. EXPRESSION ANALYSIS OF CCBE SPLICING GENES IN PICHIA PATORIS -- ACKNOWLEDGMENTS -- REFERENCE -- INDEX -- Blank Page.
|
| Record Nr. | UNINA-9910816291903321 |
Info
Chitosan hydrolysis by non-specific enzymes [[electronic resource] /] / Wenshui Xia and Ping Liu
