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Biofouling methods / / edited by Sergey Dobretsov, Jeremy C. Thomason, David N. Williams
Biofouling methods / / edited by Sergey Dobretsov, Jeremy C. Thomason, David N. Williams
Edizione [First edition.]
Pubbl/distr/stampa West Sussex, England : , : John Wiley & Sons, Ltd., , 2014
Descrizione fisica 1 online resource (411 p.)
Disciplina 628.9/6
Soggetto topico Fouling
Fouling organisms
ISBN 1-118-33611-9
1-118-33614-3
1-118-33613-5
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Biofouling Methods; Copyright; Contents; List of contributors; Introduction; Guide to methods; Part I Methods for Microfouling ; Chapter 1 Microscopy of biofilms; Section 1: Traditional light and epifluorescent microscopy; 1.1 Introduction; 1.2 Determination of bacterial abundance; 1.3 Catalyzed reporter deposition fluorescent in situ hybridization (CARD-FISH); 1.4 Suggestions, with examples, for data analysis and presentation; Acknowledgements; References; Chapter 2 Traditional and bulk methods for biofilms; Section 1: Traditional microbiological methods; 2.1 Introduction
2.2 Enrichment culture, isolation of microbes 2.3 Counting methods; 2.4 Troubleshooting hints and tips; References; Section 2 Bulk methods; 2.5 Introduction; 2.6 Measurement of biofilm thickness; 2.7 Biofilm dry weight determination; 2.8 Biofilm ATP content; 2.9 Troubleshooting hints and tips; Acknowledgements; References; Chapter 3 Biocide testing against microbes; Section 1: Testing biocides in solution: flow cytometry for planktonic stages; 3.1 Introduction; 3.2 Method introductions; 3.3 Pros and cons; 3.4 Materials and equipment; 3.5 Methods; 3.6 Troubleshooting hints and tips
3.7 Suggestions References; Section 2 Biocide testing using single and multispecies biofilms; 3.8 Introduction; 3.9 Questions to answer when applying biocides; 3.10 Laboratory methods for testing biocide effect; 3.11 Field methods for testing biocide effect; 3.12 Troubleshooting hints and tips; Acknowledgements; References; Chapter 4 Molecular methods for biofilms; Section 1: Isolation of nucleic acids; 4.1 Introduction; 4.2 Materials; 4.3 Isolation of DNA from a biofilm; 4.4 Troubleshooting hints and tips; References; Section 2 PCR and DNA sequencing
4.5 PCR and DNA sequencing: General introduction 4.6 PCR; 4.7 Microbial marker genes - 16S; 4.8 DNA sequencing; 4.9 454 16S amplicon pyrotag sequencing; 4.10 Protocol 1: DNA extraction using the Qiagen DNeasy Plant Mini Kit; 4.11 Protocol 2: Full-length 16S PCR using the Qiagen Multiplex Kit; 4.12 Protocol 3: Analysis of full-length 16S genes; 4.13 Protocol 4: 16S amplicon PCR for 454 sequencing using the Qiagen Multiplex Kit; 4.14 Protocol 5: Trimming and filtering of 454 16S pyrotag sequencing; 4.15 Protocol 6: Taxon-based analyses; 4.16 Protocol 7: Phylogeny-based analyses; References
Section 3 Community comparison by genetic fingerprinting techniques 4.17 Introduction; 4.18 History and principles of the methods; 4.19 Advantages and limitations of fingerprinting techniques; 4.20 Materials and equipment; 4.21 Suggestions for data analysis and presentation; 4.22 Troubleshooting hints and tips; Acknowledgements; References; Section 4 Metagenomics; 4.23 Introduction and brief summary of methods; 4.24 Overview of metagenomics methods; 4.25 Method introduction; 4.26 Overview of DNA handling for BAC library construction; 4.27 BAC and Fosmid library construction
4.28 Library handling, archiving, and databasing
Record Nr. UNINA-9910132186803321
West Sussex, England : , : John Wiley & Sons, Ltd., , 2014
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Biofouling methods / / edited by Sergey Dobretsov, Jeremy C. Thomason, David N. Williams
Biofouling methods / / edited by Sergey Dobretsov, Jeremy C. Thomason, David N. Williams
Edizione [First edition.]
Pubbl/distr/stampa West Sussex, England : , : John Wiley & Sons, Ltd., , 2014
Descrizione fisica 1 online resource (411 p.)
Disciplina 628.9/6
Soggetto topico Fouling
Fouling organisms
ISBN 1-118-33611-9
1-118-33614-3
1-118-33613-5
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Biofouling Methods; Copyright; Contents; List of contributors; Introduction; Guide to methods; Part I Methods for Microfouling ; Chapter 1 Microscopy of biofilms; Section 1: Traditional light and epifluorescent microscopy; 1.1 Introduction; 1.2 Determination of bacterial abundance; 1.3 Catalyzed reporter deposition fluorescent in situ hybridization (CARD-FISH); 1.4 Suggestions, with examples, for data analysis and presentation; Acknowledgements; References; Chapter 2 Traditional and bulk methods for biofilms; Section 1: Traditional microbiological methods; 2.1 Introduction
2.2 Enrichment culture, isolation of microbes 2.3 Counting methods; 2.4 Troubleshooting hints and tips; References; Section 2 Bulk methods; 2.5 Introduction; 2.6 Measurement of biofilm thickness; 2.7 Biofilm dry weight determination; 2.8 Biofilm ATP content; 2.9 Troubleshooting hints and tips; Acknowledgements; References; Chapter 3 Biocide testing against microbes; Section 1: Testing biocides in solution: flow cytometry for planktonic stages; 3.1 Introduction; 3.2 Method introductions; 3.3 Pros and cons; 3.4 Materials and equipment; 3.5 Methods; 3.6 Troubleshooting hints and tips
3.7 Suggestions References; Section 2 Biocide testing using single and multispecies biofilms; 3.8 Introduction; 3.9 Questions to answer when applying biocides; 3.10 Laboratory methods for testing biocide effect; 3.11 Field methods for testing biocide effect; 3.12 Troubleshooting hints and tips; Acknowledgements; References; Chapter 4 Molecular methods for biofilms; Section 1: Isolation of nucleic acids; 4.1 Introduction; 4.2 Materials; 4.3 Isolation of DNA from a biofilm; 4.4 Troubleshooting hints and tips; References; Section 2 PCR and DNA sequencing
4.5 PCR and DNA sequencing: General introduction 4.6 PCR; 4.7 Microbial marker genes - 16S; 4.8 DNA sequencing; 4.9 454 16S amplicon pyrotag sequencing; 4.10 Protocol 1: DNA extraction using the Qiagen DNeasy Plant Mini Kit; 4.11 Protocol 2: Full-length 16S PCR using the Qiagen Multiplex Kit; 4.12 Protocol 3: Analysis of full-length 16S genes; 4.13 Protocol 4: 16S amplicon PCR for 454 sequencing using the Qiagen Multiplex Kit; 4.14 Protocol 5: Trimming and filtering of 454 16S pyrotag sequencing; 4.15 Protocol 6: Taxon-based analyses; 4.16 Protocol 7: Phylogeny-based analyses; References
Section 3 Community comparison by genetic fingerprinting techniques 4.17 Introduction; 4.18 History and principles of the methods; 4.19 Advantages and limitations of fingerprinting techniques; 4.20 Materials and equipment; 4.21 Suggestions for data analysis and presentation; 4.22 Troubleshooting hints and tips; Acknowledgements; References; Section 4 Metagenomics; 4.23 Introduction and brief summary of methods; 4.24 Overview of metagenomics methods; 4.25 Method introduction; 4.26 Overview of DNA handling for BAC library construction; 4.27 BAC and Fosmid library construction
4.28 Library handling, archiving, and databasing
Record Nr. UNINA-9910827657503321
West Sussex, England : , : John Wiley & Sons, Ltd., , 2014
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui