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Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 1 [[electronic resource] /] / edited by Gérald Thouand, Robert Marks
| Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 1 [[electronic resource] /] / edited by Gérald Thouand, Robert Marks |
| Edizione | [1st ed. 2014.] |
| Pubbl/distr/stampa | Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2014 |
| Descrizione fisica | 1 online resource (V, 206 p. 55 illus., 24 illus. in color.) |
| Disciplina | 572.4358 |
| Collana | Advances in Biochemical Engineering/Biotechnology |
| Soggetto topico |
Microbiology
Applied Microbiology |
| ISBN | 3-662-43385-0 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Intro -- Contents -- Part IFundamentals of Bioluminescence -- 1 Eco-Evo Bioluminescence on Land and in the Sea -- Abstract -- 1...Introduction -- 2...Function of Bioluminescence -- 2.1 Self-defense -- 2.2 Light Organ Lure -- 2.3 Intraspecific Communication -- 2.4 Camouflage -- 2.5 Searchlight -- 2.6 Functionless Bioluminescence -- 3...Terrestrial Taxa -- 3.1 On Land -- 3.2 In the Air -- 3.3 Underground: Why in Soil? -- 3.4 Fresh Water: Why so Rare? -- 4...Marine Taxa -- 4.1 Intertidal Zone -- 4.2 Coastal Water -- 4.2.1 Cypridinid Luciferin -- 4.3 Midwater: Why so Species-Rich? -- 4.3.1 Symbiotic Luminescence -- 4.3.2 Coelenterazine -- 4.4 Deep-Sea Benthos -- 5...Conclusion -- 6...Perspectives -- Acknowledgments -- References -- 2 Biochemistry and Genetics of Bacterial Bioluminescence -- Abstract -- 1...Introduction -- 2...Species of Luminous Bacteria -- 3...Biochemistry of Bacterial Luminescence -- 4...Bacterial lux Genes -- 5...Regulation of lux Operon Expression -- 6...Origin and Function of Luminescence in Bacteria -- 7...Outlook -- References -- Part IIApplications of Bioluminescence inEnvironment and Security -- 3 Application of Enzyme Bioluminescence in Ecology -- Abstract -- 1...Introduction -- 2...Principles of Bioluminescent Enzymatic Bioassays -- 2.1 The Bioassays Based on the Bacterial Coupled Enzyme System: NADH:FMN-Oxidoreductase-Luciferase -- 2.2 Enzymatic Reagents for Bioluminescent Analysis -- 2.2.1 Ways to Obtain Stable Enzyme Preparations -- 2.2.2 Design of the Reagent Enzymolum and the Procedure of Toxicological Bioassay with Increased Sensitivity to Pollutants -- Varying the Composition of the Immobilized Reagent -- Varying the Order of Components Added to the Reaction Mixture -- Incubating Enzymes in the Test Solutions -- Comparing Sensitivity of Soluble and Multicomponent Immobilized Coupled Enzyme System NADH:FMN-Oxidoreductase-Luciferase.
2.3 Biotesting Natural and Waste Waters Using the Soluble and Immobilized Coupled Enzyme System NADH: FMN-Oxidoreductase-Luciferase -- 2.4 The Reagent ''Enzymolum'' in Toxicological Bioassay of Air -- 2.5 Signal System of Enzymatic Assays: Methodological Aspects -- 2.6 How to Design New Bioluminescent Enzymatic Bioassays -- 2.7 Signal System of Enzymatic Methods for Toxicological Bioassay of Natural and Laboratory Aquatic Ecosystems -- 2.7.1 Dynamics of the Pond Ecosystem and the Microecosystem Components and the Bioassay Data -- 2.7.2 Bioluminescent Control for Blooming Water -- 2.8 Signal System of Enzymatic Assays for Toxicological Bioassay of Pesticides -- 3...Summary -- Acknowledgments -- References -- 4 Detection of Organic Compounds with Whole-Cell Bioluminescent Bioassays -- Abstract -- 1...Introduction -- 2...Detection of Organic Compounds Using Bacterial Bioluminescent Assays -- 2.1 Benzene, Toluene, Ethylbenzene, and Xylene -- 2.2 Polycyclic Aromatic Hydrocarbons -- 2.3 Alkane Aliphatic Hydrocarbons -- 2.4 Chlorinated Aliphatic Hydrocarbons -- 2.5 Biphenyl and Polychlorinated Biphenyls -- 2.6 Phenol and Derivatives -- 3...Evaluation of Organic Toxicant-Induced Health Risks Using Eukaryotic Cell-Based Bioluminescent Assays -- 3.1 Bioassays for Dioxin and Dioxin-Like Compounds -- 3.2 Bioassays for Hormonally Active Chemicals -- 3.3 Environmental Applications -- 4...Conclusions -- References -- Part IIIApplications of Bioluminescencein Agriculture and Bioprocess -- 5 Detection of Bacteria with Bioluminescent Reporter Bacteriophage -- Abstract -- 1...Introduction -- 2...Bioluminescence as Reporter System -- 3...Principles of Reporter Bacteriophage Construction and Use -- 4...Specific Bioluminescent Reporter Phages -- 4.1 Antibiotic Resistance -- 4.2 Combination of Bioluminescent Reporter Phages with Other Technologies -- 5...Summary, Conclusions, and Outlook. References -- Part IVApplications of Bioluminescencein Health -- 6 Application of Enzyme Bioluminescence for Medical Diagnostics -- Abstract -- 1...Introduction -- 2...Immunoassay: State-of-the-Art -- 2.1 Luciferases as Labels for Immunoassay -- 3...Nucleic Acid Hybridization Assay Based on Bioluminescent Reporters -- 3.1 Bioluminescent Assay of Infectious Agents -- 3.2 Bioluminescent Assay in Oncology Diagnostics -- 4...Multianalytical Bioluminescent Assay -- 5...Bioluminescent Binding Assay in Homogeneous Format -- 6...Summary, Conclusions, and Outlook -- References -- Index. |
| Record Nr. | UNINA-9910298658003321 |
| Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 2 [[electronic resource] /] / edited by Gérald Thouand, Robert Marks
| Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 2 [[electronic resource] /] / edited by Gérald Thouand, Robert Marks |
| Edizione | [1st ed. 2014.] |
| Pubbl/distr/stampa | Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2014 |
| Descrizione fisica | 1 online resource (VI, 224 p. 40 illus., 19 illus. in color.) : online resource |
| Disciplina | 572.4358 |
| Collana | Advances in Biochemical Engineering/Biotechnology |
| Soggetto topico |
Microbiology
Applied Microbiology |
| ISBN | 3-662-43619-1 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Intro -- Contents -- Part I Fundamentals of Bioluminescence -- 1 How Synthetic Biology Will Reconsider Natural Bioluminescence and Its Applications -- Abstract -- 1...Introduction -- 2...Applications -- 2.1 Illumination -- 2.2 Aesthetics -- 2.3 Communication with Humans -- 2.4 Communication with Other Engineered Systems -- 3...Feasibility of Bio-lighting -- 3.1 Photosynthetic Efficiency -- 3.2 Energy Output -- 3.3 Competing with Conventional Lighting -- 4...Bioluminescent Parts and Modules in Nature -- 4.1 Firefly Luminescence -- 4.2 Bacterial Bioluminescence -- 4.3 Other Natural Luminescence Systems -- 5...Selecting Chassis Organisms -- 6...Controlling Luminescence -- 7...Containment -- 8...Improving and Altering Luminescence -- 8.1 Alterations -- 9...Conclusions -- References -- 2 Optimization of the Firefly Luciferase Reaction for Analytical Purposes -- Abstract -- 1...Introduction -- 2...Pretreatment of Samples -- 2.1 Concentration of Bacteria -- 2.2 Dilution -- 2.3 Pretreatment to Remove Specific Pools of ATP -- 2.4 Resuscitation -- 3...Extraction of Intracellular ATP -- 4...Selection of Luciferase for Different Applications -- 4.1 Reporter Gene Assays -- 4.2 ATP Assays -- 5...Luciferin -- 5.1 d-Luciferin -- 5.2 Luciferin Analogues and Derivatives -- 6...Kinetics of the Luciferase Reaction -- 7...Optimization of Reagents -- 7.1 Optimization of Assays of ATP -- 7.2 Optimization of Assays of Luciferase -- 7.3 Optimization of Coupled Enzyme Assays -- 7.3.1 Endpoint Assays of Metabolites -- 7.3.2 Kinetic Assays of Metabolites -- 7.3.3 Kinetic Assays of ATP-Forming Enzymes -- 7.3.4 Kinetic Assays of ATP-Depleting Enzymes -- 8...Stabilization of Reagents -- 9...Calibration of Assays -- 10...Sources of Background and Ways to Reduce Them -- 11...Effect of Measuring Different Light Parameters/Read-Outs -- 12...Conclusions -- Acknowledgements -- References.
Part II Applications of Bioluminescencein Environment and Security -- 3 Evaluation of the Ecotoxicity of Pollutants with Bioluminescent Microorganisms -- Abstract -- 1...Introduction -- 2...Naturally Bioluminescent Microorganisms: Their Use in Environmental Monitoring -- 3...Recombinant Bioluminescent Microorganisms: Their Use in Environmental Monitoring -- 3.1 Specific Soil Bioreporters -- 3.2 Bioreporters Specially Designed to Assess Bioremediation Programs and Wastewater Toxicity Evaluation -- 3.3 Freshwater Bioreporters -- 3.4 Eukaryotic Bioreporters -- 3.5 Aequorin-Based Bioreporters -- 4...Relevance of QSAR Models and Hormesis in Ecotoxicology -- 4.1 Bioluminescent Microorganisms and QSAR Models -- 4.2 Hormetic Response of Bioluminescent Organisms and Its Implications in Ecotoxicity Testing -- 5...Use of Bioluminescent Microorganisms to Assess Mixture Toxicity: From Whole Toxicity Determination to Development of Conceptual Models to Study the Toxicity of Complex Mixtures of Pollutants -- 5.1 Whole Toxicity Assessment -- 5.2 Bioluminescent Microorganisms and Toxicology of Mixtures -- 6...Concluding Remarks -- References -- 4 Molecular Manipulations for Enhancing Luminescent Bioreporters Performance in the Detection of Toxic Chemicals -- Abstract -- Abstract -- 1...Introduction -- 2...Genetic Manipulations of the Host Strain and Expression System -- 3...Genetic Manipulations of the Sensing Element -- 4...Genetic Manipulations of lux Reporting Elements -- 5...Additional Genetic Manipulations and the Advent of Synthetic Biology -- 6...Summary -- References -- Part III Applications of Bioluminescencein Agriculture and Bioprocess -- 5 Biosensors, Antibiotics and Food -- Abstract -- 1...Introduction -- 2...Antibiotic Use and Associated Risks -- 3...Control of Antibiotic Use -- 4...Detection Methods of Antibiotic Residues in Food -- 4.1 Confirmatory Analysis of Antibiotic Residues. 4.2 Screening Methods for Antibiotic Residues -- 5...Biosensors for Detection of Antibiotics in Food -- 5.1 Whole-Cell Biosensors -- 5.2 Inducible Whole-Cell Biosensors for Antibiotic Detection -- 6...Conclusions -- References -- Part IV Applications of Bioluminescencein Health -- 6 Rapid In-vitro Testing for Chemotherapy Sensitivity in Leukaemia Patients -- Abstract -- 1...Introduction: Bioluminescent Biosensors for Drug Sensitivity -- 1.1 Bioluminescent Reporter Technology -- 1.2 Real-Time Monitoring of Anti-microbial Effects -- 1.3 Intracellular Anti-microbial Effects -- 2...The Clinical Need in Leukaemia -- 3...Construction of a Bacterial Biosensor for Chemotherapy Sensitivity Testing -- 3.1 Mode of Action of Target Drug -- 3.2 Construction of Cytarabine Sensitive E. Coli Host -- 3.3 Bioluminescent Biosensor Development -- 4...Validation of the Biosensor Assay EA -- 4.1 Validation of Ara-CTP Measurement -- 4.2 Assay Sensitivity and Specificity -- 4.3 Assay Reproducibility and Stability -- 5...Clinical Testing -- 6...Future Perspectives -- 6.1 Multi Drug Testing -- 6.2 Cytarabine Biosensor -- 6.3 Applications with Other Drugs -- 6.4 Additional Chemotherapy Biosensors -- 6.5 Rapid Testing in Other Cancers -- References -- Index. |
| Record Nr. | UNINA-9910298647303321 |
| Berlin, Heidelberg : , : Springer Berlin Heidelberg : , : Imprint : Springer, , 2014 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3 [[electronic resource] /] / edited by Gérald Thouand, Robert Marks
| Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3 [[electronic resource] /] / edited by Gérald Thouand, Robert Marks |
| Edizione | [1st ed. 2016.] |
| Pubbl/distr/stampa | Cham : , : Springer International Publishing : , : Imprint : Springer, , 2016 |
| Descrizione fisica | 1 online resource (VIII, 203 p. 59 illus., 51 illus. in color.) |
| Disciplina | 572.4358 |
| Collana | Advances in Biochemical Engineering/Biotechnology |
| Soggetto topico |
Microbiology
Genetic engineering Biochemical engineering Biochemistry Applied Microbiology Genetic Engineering Biochemical Engineering Animal Biochemistry |
| ISBN | 3-319-27407-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Lux and luc genes as reporter reactions : how to use them in molecular biology ? -- Measurement of bioluminescence intensity and spectrum: current physical techniques and principles -- Structure, Mechanism and Mutation of Bacterial Luciferase -- Detection of metals and organometallic compounds with microbial bioluminescent bioassays -- Bioluminescent microbial biosensors applied to on line detection of chemicals -- Let there be light! Bioluminescent imaging to study bacterial pathogenesis in live animals and plants -- Uses of the Photoprotein Aequorin in Biotechnology: Fundamental Properties and Genetic Engineering -- Whole-Cell Biosensors as Tools for the Detection of Quorum Sensing Molecules: Uses in Diagnostics and the Investigation of the Quorum Sensing Mechanism. |
| Record Nr. | UNINA-9910254053403321 |
| Cham : , : Springer International Publishing : , : Imprint : Springer, , 2016 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||