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Flow cytometry with plant cells [[electronic resource] ] : analysis of genes, chromosomes and genomes / / edited by Jaroslav Doležel, Johann Greilhuber, and Jan Suda
| Flow cytometry with plant cells [[electronic resource] ] : analysis of genes, chromosomes and genomes / / edited by Jaroslav Doležel, Johann Greilhuber, and Jan Suda |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (481 p.) |
| Disciplina | 571.62 |
| Altri autori (Persone) |
DoleželJaroslav
GreilhuberJohann SudaJan |
| Soggetto topico |
Flow cytometry
Plant cells and tissues |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-92167-6
9786610921676 3-527-61092-8 3-527-61093-6 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Flow Cytometry with Plant Cells; Contents; Preface; List of Contributors; 1 Cytometry and Cytometers: Development and Growth; Overview; 1.1 Origins; 1.2 From Absorption to Fluorescence, from Imaging to Flow; 1.2.1 Early Microspectrophotometry and Image Cytometry; 1.2.2 Fluorescence Microscopy and the Fluorescent Antibody Technique; 1.2.3 Computers Meet Cytometers: The Birth of Analytical Flow Cytometry; 1.2.4 The Development of Cell Sorting; 1.3 The Growth of Multiparameter Flow Cytometry; 1.4 Bench-tops and Behemoths: Convergent Evolution; 1.5 Image Cytometry: New Beginnings?; References
2 Principles of Flow CytometryOverview; 2.1 Introduction; 2.2 A Brief History of Flow Cytometry; 2.3 Components of a Flow Cytometer; 2.3.1 Fluidics; 2.3.2 Optics; 2.3.3 Electronic Systems; 2.4 Flow Cytometric Informatics; 2.5 Spectral Compensation; 2.6 Cell Sorting; 2.7 Calibration Issues; 2.8 Conclusions; References; 3 Flow Cytometry with Plants: an Overview; Overview; 3.1 Introduction; 3.2 Fluorescence is a Fundamental Parameter; 3.3 Pushing Plants through the Flow Cytometer; 3.3.1 Difficulties with Plants and their Cells; 3.3.2 Protoplasts are somewhat ""Easier"" than Intact Cells 3.3.3 Going for Organelles3.4 Application of Flow Cytometry in Plants; 3.4.1 Microspores and Pollen; 3.4.2 Protoplasts; 3.4.2.1 Physiological Processes; 3.4.2.2 Secondary Metabolites; 3.4.2.3 Gene Expression; 3.4.2.4 Somatic Hybrids; 3.4.2.5 DNA Transfection; 3.4.3 Cell Nuclei; 3.4.3.1 Ploidy Levels; 3.4.3.2 Aneuploidy; 3.4.3.3 B Chromosomes; 3.4.3.4 Sex Chromosomes; 3.4.3.5 Cell Cycle and Endopolyploidy; 3.4.3.6 Reproductive Pathways; 3.4.3.7 Nuclear Genome Size; 3.4.3.8 DNA Base Content; 3.4.3.9 Chromatin Composition; 3.4.3.10 Sorting of Nuclei; 3.4.4 Mitotic Chromosomes; 3.4.5 Chloroplasts 3.4.6 Mitochondria3.4.7 Plant Pathogens; 3.4.8 Aquatic Flow Cytometry; 3.5 A Flow Cytometer in Every Laboratory?; 3.6 Conclusions and Future Trends; References; 4 Nuclear DNA Content Measurement; Overview; 4.1 Introduction; 4.2 Nuclear DNA Content: Words, Concepts and Symbols; 4.2.1 Replication-Division Phases; 4.2.2 Alternation of Nuclear Phases; 4.2.3 Generative Polyploidy Levels; 4.2.4 Somatic Polyploidy; 4.3 Units for Presenting DNA Amounts and their Conversion Factors; 4.4 Sample Preparation for Flow Cytometric DNA Measurement; 4.4.1 Selection of the Tissue; 4.4.2 Reagents and Solutions 4.4.2.1 Isolation Buffers and DNA Staining4.5 Standardization; 4.5.1 Types of Standardization; 4.5.2 Requirement of Internal Standardization - a Practical Test; 4.5.3 Choice of the Appropriate Standard Species; 4.5.3.1 Biological Similarity; 4.5.3.2 Genome Size; 4.5.3.3 Nature of the Standard; 4.5.3.4 Availability; 4.5.3.5 Cytological Homogeneity; 4.5.3.6 Accessibility; 4.5.3.7 Reliability of C-Values; 4.5.4 Studies on Plant Standards; 4.5.5 Suggested Standards; 4.6 Fluorescence Inhibitors and Coatings of Debris; 4.6.1 What are Fluorescence Inhibitors and Coatings of Debris? 4.6.2 Experiments with Tannic Acid |
| Record Nr. | UNINA-9910144559603321 |
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Flow cytometry with plant cells [[electronic resource] ] : analysis of genes, chromosomes and genomes / / edited by Jaroslav Doležel, Johann Greilhuber, and Jan Suda
| Flow cytometry with plant cells [[electronic resource] ] : analysis of genes, chromosomes and genomes / / edited by Jaroslav Doležel, Johann Greilhuber, and Jan Suda |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (481 p.) |
| Disciplina | 571.62 |
| Altri autori (Persone) |
DoleželJaroslav
GreilhuberJohann SudaJan |
| Soggetto topico |
Flow cytometry
Plant cells and tissues |
| ISBN |
1-280-92167-6
9786610921676 3-527-61092-8 3-527-61093-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Flow Cytometry with Plant Cells; Contents; Preface; List of Contributors; 1 Cytometry and Cytometers: Development and Growth; Overview; 1.1 Origins; 1.2 From Absorption to Fluorescence, from Imaging to Flow; 1.2.1 Early Microspectrophotometry and Image Cytometry; 1.2.2 Fluorescence Microscopy and the Fluorescent Antibody Technique; 1.2.3 Computers Meet Cytometers: The Birth of Analytical Flow Cytometry; 1.2.4 The Development of Cell Sorting; 1.3 The Growth of Multiparameter Flow Cytometry; 1.4 Bench-tops and Behemoths: Convergent Evolution; 1.5 Image Cytometry: New Beginnings?; References
2 Principles of Flow CytometryOverview; 2.1 Introduction; 2.2 A Brief History of Flow Cytometry; 2.3 Components of a Flow Cytometer; 2.3.1 Fluidics; 2.3.2 Optics; 2.3.3 Electronic Systems; 2.4 Flow Cytometric Informatics; 2.5 Spectral Compensation; 2.6 Cell Sorting; 2.7 Calibration Issues; 2.8 Conclusions; References; 3 Flow Cytometry with Plants: an Overview; Overview; 3.1 Introduction; 3.2 Fluorescence is a Fundamental Parameter; 3.3 Pushing Plants through the Flow Cytometer; 3.3.1 Difficulties with Plants and their Cells; 3.3.2 Protoplasts are somewhat ""Easier"" than Intact Cells 3.3.3 Going for Organelles3.4 Application of Flow Cytometry in Plants; 3.4.1 Microspores and Pollen; 3.4.2 Protoplasts; 3.4.2.1 Physiological Processes; 3.4.2.2 Secondary Metabolites; 3.4.2.3 Gene Expression; 3.4.2.4 Somatic Hybrids; 3.4.2.5 DNA Transfection; 3.4.3 Cell Nuclei; 3.4.3.1 Ploidy Levels; 3.4.3.2 Aneuploidy; 3.4.3.3 B Chromosomes; 3.4.3.4 Sex Chromosomes; 3.4.3.5 Cell Cycle and Endopolyploidy; 3.4.3.6 Reproductive Pathways; 3.4.3.7 Nuclear Genome Size; 3.4.3.8 DNA Base Content; 3.4.3.9 Chromatin Composition; 3.4.3.10 Sorting of Nuclei; 3.4.4 Mitotic Chromosomes; 3.4.5 Chloroplasts 3.4.6 Mitochondria3.4.7 Plant Pathogens; 3.4.8 Aquatic Flow Cytometry; 3.5 A Flow Cytometer in Every Laboratory?; 3.6 Conclusions and Future Trends; References; 4 Nuclear DNA Content Measurement; Overview; 4.1 Introduction; 4.2 Nuclear DNA Content: Words, Concepts and Symbols; 4.2.1 Replication-Division Phases; 4.2.2 Alternation of Nuclear Phases; 4.2.3 Generative Polyploidy Levels; 4.2.4 Somatic Polyploidy; 4.3 Units for Presenting DNA Amounts and their Conversion Factors; 4.4 Sample Preparation for Flow Cytometric DNA Measurement; 4.4.1 Selection of the Tissue; 4.4.2 Reagents and Solutions 4.4.2.1 Isolation Buffers and DNA Staining4.5 Standardization; 4.5.1 Types of Standardization; 4.5.2 Requirement of Internal Standardization - a Practical Test; 4.5.3 Choice of the Appropriate Standard Species; 4.5.3.1 Biological Similarity; 4.5.3.2 Genome Size; 4.5.3.3 Nature of the Standard; 4.5.3.4 Availability; 4.5.3.5 Cytological Homogeneity; 4.5.3.6 Accessibility; 4.5.3.7 Reliability of C-Values; 4.5.4 Studies on Plant Standards; 4.5.5 Suggested Standards; 4.6 Fluorescence Inhibitors and Coatings of Debris; 4.6.1 What are Fluorescence Inhibitors and Coatings of Debris? 4.6.2 Experiments with Tannic Acid |
| Record Nr. | UNINA-9910831048703321 |
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Flow cytometry with plant cells [[electronic resource] ] : analysis of genes, chromosomes and genomes / / edited by Jaroslav Doležel, Johann Greilhuber, and Jan Suda
| Flow cytometry with plant cells [[electronic resource] ] : analysis of genes, chromosomes and genomes / / edited by Jaroslav Doležel, Johann Greilhuber, and Jan Suda |
| Pubbl/distr/stampa | Weinheim, : Wiley-VCH |
| Descrizione fisica | 1 online resource (481 p.) |
| Disciplina | 571.62 |
| Altri autori (Persone) |
DoleželJaroslav
GreilhuberJohann SudaJan |
| Soggetto topico |
Flow cytometry
Plant cells and tissues |
| ISBN |
1-280-92167-6
9786610921676 3-527-61092-8 3-527-61093-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Flow Cytometry with Plant Cells; Contents; Preface; List of Contributors; 1 Cytometry and Cytometers: Development and Growth; Overview; 1.1 Origins; 1.2 From Absorption to Fluorescence, from Imaging to Flow; 1.2.1 Early Microspectrophotometry and Image Cytometry; 1.2.2 Fluorescence Microscopy and the Fluorescent Antibody Technique; 1.2.3 Computers Meet Cytometers: The Birth of Analytical Flow Cytometry; 1.2.4 The Development of Cell Sorting; 1.3 The Growth of Multiparameter Flow Cytometry; 1.4 Bench-tops and Behemoths: Convergent Evolution; 1.5 Image Cytometry: New Beginnings?; References
2 Principles of Flow CytometryOverview; 2.1 Introduction; 2.2 A Brief History of Flow Cytometry; 2.3 Components of a Flow Cytometer; 2.3.1 Fluidics; 2.3.2 Optics; 2.3.3 Electronic Systems; 2.4 Flow Cytometric Informatics; 2.5 Spectral Compensation; 2.6 Cell Sorting; 2.7 Calibration Issues; 2.8 Conclusions; References; 3 Flow Cytometry with Plants: an Overview; Overview; 3.1 Introduction; 3.2 Fluorescence is a Fundamental Parameter; 3.3 Pushing Plants through the Flow Cytometer; 3.3.1 Difficulties with Plants and their Cells; 3.3.2 Protoplasts are somewhat ""Easier"" than Intact Cells 3.3.3 Going for Organelles3.4 Application of Flow Cytometry in Plants; 3.4.1 Microspores and Pollen; 3.4.2 Protoplasts; 3.4.2.1 Physiological Processes; 3.4.2.2 Secondary Metabolites; 3.4.2.3 Gene Expression; 3.4.2.4 Somatic Hybrids; 3.4.2.5 DNA Transfection; 3.4.3 Cell Nuclei; 3.4.3.1 Ploidy Levels; 3.4.3.2 Aneuploidy; 3.4.3.3 B Chromosomes; 3.4.3.4 Sex Chromosomes; 3.4.3.5 Cell Cycle and Endopolyploidy; 3.4.3.6 Reproductive Pathways; 3.4.3.7 Nuclear Genome Size; 3.4.3.8 DNA Base Content; 3.4.3.9 Chromatin Composition; 3.4.3.10 Sorting of Nuclei; 3.4.4 Mitotic Chromosomes; 3.4.5 Chloroplasts 3.4.6 Mitochondria3.4.7 Plant Pathogens; 3.4.8 Aquatic Flow Cytometry; 3.5 A Flow Cytometer in Every Laboratory?; 3.6 Conclusions and Future Trends; References; 4 Nuclear DNA Content Measurement; Overview; 4.1 Introduction; 4.2 Nuclear DNA Content: Words, Concepts and Symbols; 4.2.1 Replication-Division Phases; 4.2.2 Alternation of Nuclear Phases; 4.2.3 Generative Polyploidy Levels; 4.2.4 Somatic Polyploidy; 4.3 Units for Presenting DNA Amounts and their Conversion Factors; 4.4 Sample Preparation for Flow Cytometric DNA Measurement; 4.4.1 Selection of the Tissue; 4.4.2 Reagents and Solutions 4.4.2.1 Isolation Buffers and DNA Staining4.5 Standardization; 4.5.1 Types of Standardization; 4.5.2 Requirement of Internal Standardization - a Practical Test; 4.5.3 Choice of the Appropriate Standard Species; 4.5.3.1 Biological Similarity; 4.5.3.2 Genome Size; 4.5.3.3 Nature of the Standard; 4.5.3.4 Availability; 4.5.3.5 Cytological Homogeneity; 4.5.3.6 Accessibility; 4.5.3.7 Reliability of C-Values; 4.5.4 Studies on Plant Standards; 4.5.5 Suggested Standards; 4.6 Fluorescence Inhibitors and Coatings of Debris; 4.6.1 What are Fluorescence Inhibitors and Coatings of Debris? 4.6.2 Experiments with Tannic Acid |
| Record Nr. | UNINA-9910841594503321 |
| Weinheim, : Wiley-VCH | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||